Gli1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gli1
Entrez Gene ID	140589
Full Name	GLI family zinc finger 1
Synonyms	Gli
General protein information	Preferred Names GLI family zinc finger 1 Names zinc finger protein GLI1 GLI-Kruppel family member GLI1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22
Summary	mouse homolog is a zinc finger transcription factor and oncogene; involved in development [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594631.1	XP_017450120.1	zinc finger protein GLI1 isoform X1
NM_001191910.1	NP_001178839.1	zinc finger protein GLI1
XM_006241442.3	XP_006241504.1	zinc finger protein GLI1 isoform X1
XM_008765375.2	XP_008763597.1	zinc finger protein GLI1 isoform X1
XM_006241443.3	XP_006241505.1	zinc finger protein GLI1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GLI1	XP_003313606.1
Canis lupus familiaris (dog)	GLI1	XP_538247.2
Homo sapiens (human)	GLI1	NP_005260.1
Mus musculus (house mouse)	Gli1	NP_034426.2
	GLI1	XP_001116072.1
Bos taurus (cattle)	GLI1	NP_001092470.1
Rattus norvegicus (Norway rat)	Gli1	NP_001178839.1

Gene Ontology
Bibliography

Related articles in PubMed

miR-873 suppresses H9C2 cardiomyocyte proliferation by targeting GLI1.
Zhang JS, Zhao Y, Lv Y, Liu PY, Ruan JX, Sun YL, Gong TX, Wan N, Qiu GR
Gene626426-432(2017 Aug)

Sonic Hedgehog Signaling Affected by Promoter Hypermethylation Induces Aberrant Gli2 Expression in Spina Bifida.
Lu XL, Wang L, Chang SY, Shangguan SF, Wang Z, Wu LH, Zou JZ, Xiao P, Li R, Bao YH, Qiu ZY, Zhang T
Molecular neurobiology53(8)5413-24(2016 Oct)

The Effect of SHH-Gli Signaling Pathway on the Synovial Fibroblast Proliferation in Rheumatoid Arthritis.
Qin S, Sun D, Li H, Li X, Pan W, Yan C, Tang R, Liu X
Inflammation39(2)503-12(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that SHH-Gli pathway is involved in the pathogenesis of Rheumatoid arthritis, and blocking SHH-Gli pathway inhibits Rheumatoid arthritis-synovial fibroblast cell proliferation and increases cell apoptosis, which may shed light on developing new ideas for Rheumatoid arthritis treatment.
Title: The Effect of SHH-Gli Signaling Pathway on the Synovial Fibroblast Proliferation in Rheumatoid Arthritis.

This study characterizes, for the first time, the transcriptional response of cardiomyocytes to Shh and establishes a critical role for Smo coupling to Gi in Hh signaling in the normal and ischemic myocardium
Title: Gi proteins mediate activation of the canonical hedgehog pathway in the myocardium.

The expression of Shh, Smo and Gli1 at the protein and mRNA level in hepatocytes of rats with chronic fluorosis can be increased by fluoride and may be inhibited by cyclopamine.
Title: Protein and mRNA expression of Shh, Smo and Gli1 and inhibition by cyclopamine in hepatocytes of rats with chronic fluorosis.

The present results suggested that inhibition of Shh signaling pathway exacerbated rat ischemic damage caused by pMCAO, which may be correlated with down-regulated expression of Gli1, Ptch1 and SOD1.
Title: Inhibition of sonic hedgehog signaling aggravates brain damage associated with the down-regulation of Gli1, Ptch1 and SOD1 expression in acute ischemic stroke.

Shh, Ptch1, and Gli1 were expressed in rat pulmonary microvascular endothelial cells and their expression decreased when cells were treated by lipopolysaccharide.
Title: Protection from lipopolysaccharide-induced pulmonary microvascular endothelial cell injury by activation of hedgehog signaling pathway.

The following Gli1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gli1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12602	NM_001191910.1 Latest version!	Rattus norvegicus GLI family zinc finger 1 (Gli1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
ORa12602	XM_006241443.3 Latest version!	Rattus norvegicus GLI family zinc finger 1 (Gli1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
ORa12602	XM_017594631.1 Latest version!	Rattus norvegicus GLI family zinc finger 1 (Gli1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
ORa12602	XM_008765375.2 Latest version!	Rattus norvegicus GLI family zinc finger 1 (Gli1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
ORa12602	XM_006241442.3 Latest version!	Rattus norvegicus GLI family zinc finger 1 (Gli1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gli1 ( NM_001191910.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178839.1 Rattus norvegicus GLI family zinc finger 1 (Gli1), mRNA.

Price & Availability↑

CloneID	ORa12602
Clone ID Related Accession (Same CDS sequence)	XM_017594631.1 , NM_001191910.1 , XM_006241442.3 , XM_008765375.2 , XM_006241443.3
Accession Version	NM_001191910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein GLI1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC114111.6. On Jul 17, 2010 this sequence version replaced XM_345832.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTTCAATC CAATGACTTC ACCACAAGTC AATAGCTATG GTGAGCCATG CTGTCTCCGA 
CCCCTCCCCA GCCAAGGGGT CCCCAGCATG GGAACAGAAG GACTTTCTGG TCTGCCCTTT 
TGCCACCAAG CCAATTTTAT GTCAGGGTCC CAGGGTTATG GGTCTGCCAG AGAGACCAGC 
AGCTGCACTG AGGGGTCACT CTTTCCTCCT CCTCGGAGTT CAGTCAAACT GACTAAGAAG 
CGGGCTCTTT CCATCTCGCC TCTCTCTGAC GCCAGCCTCG ACCTGCAAAC CGTAATCCGT 
ACCTCACCCA GCTCCCTCGT GGCTTTCATC AACTCTCGTT GTACATCTCC AGGCGGTTCC 
TACGGCCATC TCTCCATTGG CACCATGAGC CCTTCTTTAG GATTCCCACC TCAGATGAGT 
CACCAGAAAG GAACTTCACC TCCCTATGGA GTCCAGCCCT GTGTTCCACA TGATTCTACT 
CAGGGTTCAA TGATGCTTCA CCCCCAGTCC CGAGGACCAC GTGCAACCTG CCAGCTGAAG 
TCAGAGCTGG ACATCATGGT TGGCAAGTGC CCAGAGGAAC CTTTGGAAGG GGACATGTCT 
AGCCCCAACT CCACGAGCAC ACAGGATCAC CTGCTGGGGA TGCTGGATGG ACGGGAGGAC 
CTGGAGAGAG AGGAGAAACC TGAGCCTGAG TCTGTGTATG AGACGGACTG CCGCTGGGAT 
GGTTGCAGCC AGGAGTTCGA TTCCCAAGAA CAGCTGGTGC ACCACATCAA CAGTGAGCAC 
ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGAG GCTGCTCCAG GGAGCTGAGG 
CCCTTCAAGG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA 
CACAAGTGCA CGTTTGAAGG CTGTCGGAAG TCCTATTCAC GCCTCGAAAA CCTGAAGACA 
CACCTTCGGT CGCACACCGG TGAGAAGCCT TATATGTGTG AGCAGGAAGG CTGCAGCAAG 
GCCTTCAGCA ACGCCAGTGA CCGTGCCAAG CACCAGAATA GGACCCACTC CAATGAGAAG 
CCATATGTGT GCAAACTCCC CGGCTGCACC AAGCGCTACA CAGATCCCAG CTCGCTCCGC 
AAACATGTCA AGACTGTGCA CGGTCCGGAT GCCCACGTGA CCAAACGACA TCGAGGGGAT 
GGCCCCTTAC CACGGGCACA GCCCCTCTCC ACAGTGGAGC CCAAGCGAGA AAGGGAAGGA 
GGATCCGGCA GGGAAGAGAG CAGACTGACT GTGCCGGAGG GCACCATGCC TCAGCAGAGC 
CCTGGAGCAC AATCCTCCTG CAGCAGTGAC CACTCCCCAG CAGGCAGCGC GGCCAACACG 
GACAGCGGCG TGGAGATGGC CGGCAACGCA GGGGGCAGCA CTGAGGACTT GTCCAGCTTG 
GATGAAGGAC CTTGCGTTGC GGCTACCGGA CTCTCCACCC TTCGCCGCCT TGAGAACCTC 
AGGCTGGATC AGCTGCATCA GCTCCGGCCA ATAGGGTCCC GGGGTCTCAA ACTGCCCAGC 
TTAACCCACG CTGGCGCACC TGTGTCTCGA CGTCTGGGCC CACCGGTCTC CCTTGACCGC 
CGCAGCAGCA GCTCCAGCAG CATTAGCTCC GCGTACACAG TCAGCCGCCG GTCCTCGCTG 
GCATCCCCGT TCCCGCCGGG ATCCCCACCA GAGAACGGGG CGTCGTCACT ACCTGGCCTC 
ACACCTGCCC AGCACTACAT GCTCCGGGCA AGATATGCTT CAGCCAGGGG GAGTGGCACC 
CCACCCACTG CAGCACACAG CCTGGATCGG ATCGGGGGTC TTCCTGCACC TCCTTGGAGA 
AGCCGAGCTG AGTACCCAGG ATACAACCCC AATGCAGGGG TCACGAGGAG GGCCAGTGAC 
CCAGCCCGGG CTGCTGACCA CCCGGCTCCA GCTAGAGTAC AACGGTTCAA GAGCTTGGGG 
TGTGTACACA CGCCTCCTAG TGTGGCAACA GGACGGAACT TCGATCCCCA CCTCCCTACC 
TCTGTCTATT CGCCACAGCC CCCCAGCATC ACCGAAAATG TTGCTATGGA TACTAGAGGG 
CTACAGGGGG AGCTAGAGGT TGGAACTTCC ATGATGGGCA ATGATCTGAA CCCATACATG 
GACTTTTCTT CTGCTGACAC TCTGGGATAT GGGGGGCCTG AGGGGACGGC GGCTGAGTCT 
TATGGATCTA AGGGTCCAGG TTCCCTGCCT CTTGGGCCTG GTCCACCAAC CAACTATGGC 
CCTGGCCACT GTGCCCAGCA GGTCTCCTAT CCTGATCCCA CCCCAGAAAC ATGGGGTGAG 
TTTCCTTCAC ATGCTGGGGT GTACCCTGGC ACTAAGGCTC TAGGTACAGC CTATAACCAG 
TGTCCTCGAC TTGAGCATTA TGGACAAGTG CAAGTAAAAC CAGAACAAGG GTGCCCAGTG 
GGGTCTGACT CCACCGGGTT GGCACCCTGC CTCAATGCCC ACCCCAGTGA AGGGTCCCCA 
GACCCACAGC CTCTGTTTTC ACATCACTCC CAGCTCCCTC CACCCCAGTA TCTCCAGTCA 
GGTCCCTACC CTCAGCCTCC CCATGACTGT CTCTCGTTAG AATCCAGGCC TGGCCTCAAT 
TTCAACCCCT CCTCTCATTC CACAGGACAG CTCAAAGCTC AGCTCCTGTG TAATTACGTT 
CAGTCGCAGC AGGAATTGTT GTGGGAGGGC AGAAGCCGGG GAGGGATCCC CAACCAGGAA 
CTTCCATATC AGAGCCCCAA GTTTCTGGGA GGTTCTCAAG TTAGTCAGAG CCCCACCAAG 
GTCCCAGCAG CAGCAGCAGC AGCTACGTTT GGATCTGGCT TTGCATCACC TGCTTTGGCC 
AATCACAAAT CAGTCTCCTA TCCTGCCCCT TCACCGTGCC ATGAAACTTT CACCATGGGA 
GTAAACAGGC CTCCCCATAG GCCAGCAGCA CCACCCCGGC TTCTGCCCCC GCTACCACCT 
TGCTATGGGC CCCTCAAGGT GGGAGATGCC AACCCCAGCT GTGGGCATCC TGAAGTGGGC 
AGGTTAGGAG CAGGCCCTGC CTTGTACCCT CCCCCTGAAG GGCAGGTGTG TAACCCTCTG 
GACTCTCTTG ACCTGGACAA CACTCAGCTG GACTTTGTGG CTATCCTAGA TGAAGCTCAA 
GGGCTGAGCC CTCCTCCTTC CCACGAGCAA GGGGACAGCT CTAAAAACAC CCCACCTCCC 
TCCGGGCCCC CCAACATGGC AGTCGGTAAC ATGAGTGTCT TACTGGGGTC TCTGCCTGGA 
GAGACGCAGT TCCTCAACTC TAGTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178839.1
CDS	1..3330
Translation	MFNPMTSPQVNSYGEPCCLRPLPSQGVPSMGTEGLSGLPFCHQANFMSGSQGYGSARETSSCTEGSLFPPPRSSVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPPQMSHQKGTSPPYGVQPCVPHDSTQGSMMLHPQSRGPRATCQLKSELDIMVGKCPEEPLEGDMSSPNSTSTQDHLLGMLDGREDLEREEKPEPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEQEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAQPLSTVEPKREREGGSGREESRLTVPEGTMPQQSPGAQSSCSSDHSPAGSAANTDSGVEMAGNAGGSTEDLSSLDEGPCVAATGLSTLRRLENLRLDQLHQLRPIGSRGLKLPSLTHAGAPVSRRLGPPVSLDRRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSLPGLTPAQHYMLRARYASARGSGTPPTAAHSLDRIGGLPAPPWRSRAEYPGYNPNAGVTRRASDPARAADHPAPARVQRFKSLGCVHTPPSVATGRNFDPHLPTSVYSPQPPSITENVAMDTRGLQGELEVGTSMMGNDLNPYMDFSSADTLGYGGPEGTAAESYGSKGPGSLPLGPGPPTNYGPGHCAQQVSYPDPTPETWGEFPSHAGVYPGTKALGTAYNQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGSPDPQPLFSHHSQLPPPQYLQSGPYPQPPHDCLSLESRPGLNFNPSSHSTGQLKAQLLCNYVQSQQELLWEGRSRGGIPNQELPYQSPKFLGGSQVSQSPTKVPAAAAAATFGSGFASPALANHKSVSYPAPSPCHETFTMGVNRPPHRPAAPPRLLPPLPPCYGPLKVGDANPSCGHPEVGRLGAGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEAQGLSPPPSHEQGDSSKNTPPPSGPPNMAVGNMSVLLGSLPGETQFLNSSA

Target ORF information:

RefSeq Version	NM_001191910.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GLI family zinc finger 1 (Gli1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191910.1

ORF Insert Sequence:

ATGTTCAATC CAATGACTTC ACCACAAGTC AATAGCTATG GTGAGCCATG CTGTCTCCGA 
CCCCTCCCCA GCCAAGGGGT CCCCAGCATG GGAACAGAAG GACTTTCTGG TCTGCCCTTT 
TGCCACCAAG CCAATTTTAT GTCAGGGTCC CAGGGTTATG GGTCTGCCAG AGAGACCAGC 
AGCTGCACTG AGGGGTCACT CTTTCCTCCT CCTCGGAGTT CAGTCAAACT GACTAAGAAG 
CGGGCTCTTT CCATCTCGCC TCTCTCTGAC GCCAGCCTCG ACCTGCAAAC CGTAATCCGT 
ACCTCACCCA GCTCCCTCGT GGCTTTCATC AACTCTCGTT GTACATCTCC AGGCGGTTCC 
TACGGCCATC TCTCCATTGG CACCATGAGC CCTTCTTTAG GATTCCCACC TCAGATGAGT 
CACCAGAAAG GAACTTCACC TCCCTATGGA GTCCAGCCCT GTGTTCCACA TGATTCTACT 
CAGGGTTCAA TGATGCTTCA CCCCCAGTCC CGAGGACCAC GTGCAACCTG CCAGCTGAAG 
TCAGAGCTGG ACATCATGGT TGGCAAGTGC CCAGAGGAAC CTTTGGAAGG GGACATGTCT 
AGCCCCAACT CCACGAGCAC ACAGGATCAC CTGCTGGGGA TGCTGGATGG ACGGGAGGAC 
CTGGAGAGAG AGGAGAAACC TGAGCCTGAG TCTGTGTATG AGACGGACTG CCGCTGGGAT 
GGTTGCAGCC AGGAGTTCGA TTCCCAAGAA CAGCTGGTGC ACCACATCAA CAGTGAGCAC 
ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGAG GCTGCTCCAG GGAGCTGAGG 
CCCTTCAAGG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA 
CACAAGTGCA CGTTTGAAGG CTGTCGGAAG TCCTATTCAC GCCTCGAAAA CCTGAAGACA 
CACCTTCGGT CGCACACCGG TGAGAAGCCT TATATGTGTG AGCAGGAAGG CTGCAGCAAG 
GCCTTCAGCA ACGCCAGTGA CCGTGCCAAG CACCAGAATA GGACCCACTC CAATGAGAAG 
CCATATGTGT GCAAACTCCC CGGCTGCACC AAGCGCTACA CAGATCCCAG CTCGCTCCGC 
AAACATGTCA AGACTGTGCA CGGTCCGGAT GCCCACGTGA CCAAACGACA TCGAGGGGAT 
GGCCCCTTAC CACGGGCACA GCCCCTCTCC ACAGTGGAGC CCAAGCGAGA AAGGGAAGGA 
GGATCCGGCA GGGAAGAGAG CAGACTGACT GTGCCGGAGG GCACCATGCC TCAGCAGAGC 
CCTGGAGCAC AATCCTCCTG CAGCAGTGAC CACTCCCCAG CAGGCAGCGC GGCCAACACG 
GACAGCGGCG TGGAGATGGC CGGCAACGCA GGGGGCAGCA CTGAGGACTT GTCCAGCTTG 
GATGAAGGAC CTTGCGTTGC GGCTACCGGA CTCTCCACCC TTCGCCGCCT TGAGAACCTC 
AGGCTGGATC AGCTGCATCA GCTCCGGCCA ATAGGGTCCC GGGGTCTCAA ACTGCCCAGC 
TTAACCCACG CTGGCGCACC TGTGTCTCGA CGTCTGGGCC CACCGGTCTC CCTTGACCGC 
CGCAGCAGCA GCTCCAGCAG CATTAGCTCC GCGTACACAG TCAGCCGCCG GTCCTCGCTG 
GCATCCCCGT TCCCGCCGGG ATCCCCACCA GAGAACGGGG CGTCGTCACT ACCTGGCCTC 
ACACCTGCCC AGCACTACAT GCTCCGGGCA AGATATGCTT CAGCCAGGGG GAGTGGCACC 
CCACCCACTG CAGCACACAG CCTGGATCGG ATCGGGGGTC TTCCTGCACC TCCTTGGAGA 
AGCCGAGCTG AGTACCCAGG ATACAACCCC AATGCAGGGG TCACGAGGAG GGCCAGTGAC 
CCAGCCCGGG CTGCTGACCA CCCGGCTCCA GCTAGAGTAC AACGGTTCAA GAGCTTGGGG 
TGTGTACACA CGCCTCCTAG TGTGGCAACA GGACGGAACT TCGATCCCCA CCTCCCTACC 
TCTGTCTATT CGCCACAGCC CCCCAGCATC ACCGAAAATG TTGCTATGGA TACTAGAGGG 
CTACAGGGGG AGCTAGAGGT TGGAACTTCC ATGATGGGCA ATGATCTGAA CCCATACATG 
GACTTTTCTT CTGCTGACAC TCTGGGATAT GGGGGGCCTG AGGGGACGGC GGCTGAGTCT 
TATGGATCTA AGGGTCCAGG TTCCCTGCCT CTTGGGCCTG GTCCACCAAC CAACTATGGC 
CCTGGCCACT GTGCCCAGCA GGTCTCCTAT CCTGATCCCA CCCCAGAAAC ATGGGGTGAG 
TTTCCTTCAC ATGCTGGGGT GTACCCTGGC ACTAAGGCTC TAGGTACAGC CTATAACCAG 
TGTCCTCGAC TTGAGCATTA TGGACAAGTG CAAGTAAAAC CAGAACAAGG GTGCCCAGTG 
GGGTCTGACT CCACCGGGTT GGCACCCTGC CTCAATGCCC ACCCCAGTGA AGGGTCCCCA 
GACCCACAGC CTCTGTTTTC ACATCACTCC CAGCTCCCTC CACCCCAGTA TCTCCAGTCA 
GGTCCCTACC CTCAGCCTCC CCATGACTGT CTCTCGTTAG AATCCAGGCC TGGCCTCAAT 
TTCAACCCCT CCTCTCATTC CACAGGACAG CTCAAAGCTC AGCTCCTGTG TAATTACGTT 
CAGTCGCAGC AGGAATTGTT GTGGGAGGGC AGAAGCCGGG GAGGGATCCC CAACCAGGAA 
CTTCCATATC AGAGCCCCAA GTTTCTGGGA GGTTCTCAAG TTAGTCAGAG CCCCACCAAG 
GTCCCAGCAG CAGCAGCAGC AGCTACGTTT GGATCTGGCT TTGCATCACC TGCTTTGGCC 
AATCACAAAT CAGTCTCCTA TCCTGCCCCT TCACCGTGCC ATGAAACTTT CACCATGGGA 
GTAAACAGGC CTCCCCATAG GCCAGCAGCA CCACCCCGGC TTCTGCCCCC GCTACCACCT 
TGCTATGGGC CCCTCAAGGT GGGAGATGCC AACCCCAGCT GTGGGCATCC TGAAGTGGGC 
AGGTTAGGAG CAGGCCCTGC CTTGTACCCT CCCCCTGAAG GGCAGGTGTG TAACCCTCTG 
GACTCTCTTG ACCTGGACAA CACTCAGCTG GACTTTGTGG CTATCCTAGA TGAAGCTCAA 
GGGCTGAGCC CTCCTCCTTC CCACGAGCAA GGGGACAGCT CTAAAAACAC CCCACCTCCC 
TCCGGGCCCC CCAACATGGC AGTCGGTAAC ATGAGTGTCT TACTGGGGTC TCTGCCTGGA 
GAGACGCAGT TCCTCAACTC TAGTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli1 ( XM_017594631.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450120.1 Rattus norvegicus GLI family zinc finger 1 (Gli1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa12602
Clone ID Related Accession (Same CDS sequence)	XM_017594631.1 , NM_001191910.1 , XM_006241442.3 , XM_008765375.2 , XM_006241443.3
Accession Version	XM_017594631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein GLI1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAATC CAATGACTTC ACCACAAGTC AATAGCTATG GTGAGCCATG CTGTCTCCGA 
CCCCTCCCCA GCCAAGGGGT CCCCAGCATG GGAACAGAAG GACTTTCTGG TCTGCCCTTT 
TGCCACCAAG CCAATTTTAT GTCAGGGTCC CAGGGTTATG GGTCTGCCAG AGAGACCAGC 
AGCTGCACTG AGGGGTCACT CTTTCCTCCT CCTCGGAGTT CAGTCAAACT GACTAAGAAG 
CGGGCTCTTT CCATCTCGCC TCTCTCTGAC GCCAGCCTCG ACCTGCAAAC CGTAATCCGT 
ACCTCACCCA GCTCCCTCGT GGCTTTCATC AACTCTCGTT GTACATCTCC AGGCGGTTCC 
TACGGCCATC TCTCCATTGG CACCATGAGC CCTTCTTTAG GATTCCCACC TCAGATGAGT 
CACCAGAAAG GAACTTCACC TCCCTATGGA GTCCAGCCCT GTGTTCCACA TGATTCTACT 
CAGGGTTCAA TGATGCTTCA CCCCCAGTCC CGAGGACCAC GTGCAACCTG CCAGCTGAAG 
TCAGAGCTGG ACATCATGGT TGGCAAGTGC CCAGAGGAAC CTTTGGAAGG GGACATGTCT 
AGCCCCAACT CCACGAGCAC ACAGGATCAC CTGCTGGGGA TGCTGGATGG ACGGGAGGAC 
CTGGAGAGAG AGGAGAAACC TGAGCCTGAG TCTGTGTATG AGACGGACTG CCGCTGGGAT 
GGTTGCAGCC AGGAGTTCGA TTCCCAAGAA CAGCTGGTGC ACCACATCAA CAGTGAGCAC 
ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGAG GCTGCTCCAG GGAGCTGAGG 
CCCTTCAAGG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA 
CACAAGTGCA CGTTTGAAGG CTGTCGGAAG TCCTATTCAC GCCTCGAAAA CCTGAAGACA 
CACCTTCGGT CGCACACCGG TGAGAAGCCT TATATGTGTG AGCAGGAAGG CTGCAGCAAG 
GCCTTCAGCA ACGCCAGTGA CCGTGCCAAG CACCAGAATA GGACCCACTC CAATGAGAAG 
CCATATGTGT GCAAACTCCC CGGCTGCACC AAGCGCTACA CAGATCCCAG CTCGCTCCGC 
AAACATGTCA AGACTGTGCA CGGTCCGGAT GCCCACGTGA CCAAACGACA TCGAGGGGAT 
GGCCCCTTAC CACGGGCACA GCCCCTCTCC ACAGTGGAGC CCAAGCGAGA AAGGGAAGGA 
GGATCCGGCA GGGAAGAGAG CAGACTGACT GTGCCGGAGG GCACCATGCC TCAGCAGAGC 
CCTGGAGCAC AATCCTCCTG CAGCAGTGAC CACTCCCCAG CAGGCAGCGC GGCCAACACG 
GACAGCGGCG TGGAGATGGC CGGCAACGCA GGGGGCAGCA CTGAGGACTT GTCCAGCTTG 
GATGAAGGAC CTTGCGTTGC GGCTACCGGA CTCTCCACCC TTCGCCGCCT TGAGAACCTC 
AGGCTGGATC AGCTGCATCA GCTCCGGCCA ATAGGGTCCC GGGGTCTCAA ACTGCCCAGC 
TTAACCCACG CTGGCGCACC TGTGTCTCGA CGTCTGGGCC CACCGGTCTC CCTTGACCGC 
CGCAGCAGCA GCTCCAGCAG CATTAGCTCC GCGTACACAG TCAGCCGCCG GTCCTCGCTG 
GCATCCCCGT TCCCGCCGGG ATCCCCACCA GAGAACGGGG CGTCGTCACT ACCTGGCCTC 
ACACCTGCCC AGCACTACAT GCTCCGGGCA AGATATGCTT CAGCCAGGGG GAGTGGCACC 
CCACCCACTG CAGCACACAG CCTGGATCGG ATCGGGGGTC TTCCTGCACC TCCTTGGAGA 
AGCCGAGCTG AGTACCCAGG ATACAACCCC AATGCAGGGG TCACGAGGAG GGCCAGTGAC 
CCAGCCCGGG CTGCTGACCA CCCGGCTCCA GCTAGAGTAC AACGGTTCAA GAGCTTGGGG 
TGTGTACACA CGCCTCCTAG TGTGGCAACA GGACGGAACT TCGATCCCCA CCTCCCTACC 
TCTGTCTATT CGCCACAGCC CCCCAGCATC ACCGAAAATG TTGCTATGGA TACTAGAGGG 
CTACAGGGGG AGCTAGAGGT TGGAACTTCC ATGATGGGCA ATGATCTGAA CCCATACATG 
GACTTTTCTT CTGCTGACAC TCTGGGATAT GGGGGGCCTG AGGGGACGGC GGCTGAGTCT 
TATGGATCTA AGGGTCCAGG TTCCCTGCCT CTTGGGCCTG GTCCACCAAC CAACTATGGC 
CCTGGCCACT GTGCCCAGCA GGTCTCCTAT CCTGATCCCA CCCCAGAAAC ATGGGGTGAG 
TTTCCTTCAC ATGCTGGGGT GTACCCTGGC ACTAAGGCTC TAGGTACAGC CTATAACCAG 
TGTCCTCGAC TTGAGCATTA TGGACAAGTG CAAGTAAAAC CAGAACAAGG GTGCCCAGTG 
GGGTCTGACT CCACCGGGTT GGCACCCTGC CTCAATGCCC ACCCCAGTGA AGGGTCCCCA 
GACCCACAGC CTCTGTTTTC ACATCACTCC CAGCTCCCTC CACCCCAGTA TCTCCAGTCA 
GGTCCCTACC CTCAGCCTCC CCATGACTGT CTCTCGTTAG AATCCAGGCC TGGCCTCAAT 
TTCAACCCCT CCTCTCATTC CACAGGACAG CTCAAAGCTC AGCTCCTGTG TAATTACGTT 
CAGTCGCAGC AGGAATTGTT GTGGGAGGGC AGAAGCCGGG GAGGGATCCC CAACCAGGAA 
CTTCCATATC AGAGCCCCAA GTTTCTGGGA GGTTCTCAAG TTAGTCAGAG CCCCACCAAG 
GTCCCAGCAG CAGCAGCAGC AGCTACGTTT GGATCTGGCT TTGCATCACC TGCTTTGGCC 
AATCACAAAT CAGTCTCCTA TCCTGCCCCT TCACCGTGCC ATGAAACTTT CACCATGGGA 
GTAAACAGGC CTCCCCATAG GCCAGCAGCA CCACCCCGGC TTCTGCCCCC GCTACCACCT 
TGCTATGGGC CCCTCAAGGT GGGAGATGCC AACCCCAGCT GTGGGCATCC TGAAGTGGGC 
AGGTTAGGAG CAGGCCCTGC CTTGTACCCT CCCCCTGAAG GGCAGGTGTG TAACCCTCTG 
GACTCTCTTG ACCTGGACAA CACTCAGCTG GACTTTGTGG CTATCCTAGA TGAAGCTCAA 
GGGCTGAGCC CTCCTCCTTC CCACGAGCAA GGGGACAGCT CTAAAAACAC CCCACCTCCC 
TCCGGGCCCC CCAACATGGC AGTCGGTAAC ATGAGTGTCT TACTGGGGTC TCTGCCTGGA 
GAGACGCAGT TCCTCAACTC TAGTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450120.1
CDS	375..3704
Translation	MFNPMTSPQVNSYGEPCCLRPLPSQGVPSMGTEGLSGLPFCHQANFMSGSQGYGSARETSSCTEGSLFPPPRSSVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPPQMSHQKGTSPPYGVQPCVPHDSTQGSMMLHPQSRGPRATCQLKSELDIMVGKCPEEPLEGDMSSPNSTSTQDHLLGMLDGREDLEREEKPEPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEQEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAQPLSTVEPKREREGGSGREESRLTVPEGTMPQQSPGAQSSCSSDHSPAGSAANTDSGVEMAGNAGGSTEDLSSLDEGPCVAATGLSTLRRLENLRLDQLHQLRPIGSRGLKLPSLTHAGAPVSRRLGPPVSLDRRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSLPGLTPAQHYMLRARYASARGSGTPPTAAHSLDRIGGLPAPPWRSRAEYPGYNPNAGVTRRASDPARAADHPAPARVQRFKSLGCVHTPPSVATGRNFDPHLPTSVYSPQPPSITENVAMDTRGLQGELEVGTSMMGNDLNPYMDFSSADTLGYGGPEGTAAESYGSKGPGSLPLGPGPPTNYGPGHCAQQVSYPDPTPETWGEFPSHAGVYPGTKALGTAYNQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGSPDPQPLFSHHSQLPPPQYLQSGPYPQPPHDCLSLESRPGLNFNPSSHSTGQLKAQLLCNYVQSQQELLWEGRSRGGIPNQELPYQSPKFLGGSQVSQSPTKVPAAAAAATFGSGFASPALANHKSVSYPAPSPCHETFTMGVNRPPHRPAAPPRLLPPLPPCYGPLKVGDANPSCGHPEVGRLGAGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEAQGLSPPPSHEQGDSSKNTPPPSGPPNMAVGNMSVLLGSLPGETQFLNSSA

Target ORF information:

RefSeq Version	XM_017594631.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GLI family zinc finger 1 (Gli1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594631.1

ORF Insert Sequence:

ATGTTCAATC CAATGACTTC ACCACAAGTC AATAGCTATG GTGAGCCATG CTGTCTCCGA 
CCCCTCCCCA GCCAAGGGGT CCCCAGCATG GGAACAGAAG GACTTTCTGG TCTGCCCTTT 
TGCCACCAAG CCAATTTTAT GTCAGGGTCC CAGGGTTATG GGTCTGCCAG AGAGACCAGC 
AGCTGCACTG AGGGGTCACT CTTTCCTCCT CCTCGGAGTT CAGTCAAACT GACTAAGAAG 
CGGGCTCTTT CCATCTCGCC TCTCTCTGAC GCCAGCCTCG ACCTGCAAAC CGTAATCCGT 
ACCTCACCCA GCTCCCTCGT GGCTTTCATC AACTCTCGTT GTACATCTCC AGGCGGTTCC 
TACGGCCATC TCTCCATTGG CACCATGAGC CCTTCTTTAG GATTCCCACC TCAGATGAGT 
CACCAGAAAG GAACTTCACC TCCCTATGGA GTCCAGCCCT GTGTTCCACA TGATTCTACT 
CAGGGTTCAA TGATGCTTCA CCCCCAGTCC CGAGGACCAC GTGCAACCTG CCAGCTGAAG 
TCAGAGCTGG ACATCATGGT TGGCAAGTGC CCAGAGGAAC CTTTGGAAGG GGACATGTCT 
AGCCCCAACT CCACGAGCAC ACAGGATCAC CTGCTGGGGA TGCTGGATGG ACGGGAGGAC 
CTGGAGAGAG AGGAGAAACC TGAGCCTGAG TCTGTGTATG AGACGGACTG CCGCTGGGAT 
GGTTGCAGCC AGGAGTTCGA TTCCCAAGAA CAGCTGGTGC ACCACATCAA CAGTGAGCAC 
ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGAG GCTGCTCCAG GGAGCTGAGG 
CCCTTCAAGG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA 
CACAAGTGCA CGTTTGAAGG CTGTCGGAAG TCCTATTCAC GCCTCGAAAA CCTGAAGACA 
CACCTTCGGT CGCACACCGG TGAGAAGCCT TATATGTGTG AGCAGGAAGG CTGCAGCAAG 
GCCTTCAGCA ACGCCAGTGA CCGTGCCAAG CACCAGAATA GGACCCACTC CAATGAGAAG 
CCATATGTGT GCAAACTCCC CGGCTGCACC AAGCGCTACA CAGATCCCAG CTCGCTCCGC 
AAACATGTCA AGACTGTGCA CGGTCCGGAT GCCCACGTGA CCAAACGACA TCGAGGGGAT 
GGCCCCTTAC CACGGGCACA GCCCCTCTCC ACAGTGGAGC CCAAGCGAGA AAGGGAAGGA 
GGATCCGGCA GGGAAGAGAG CAGACTGACT GTGCCGGAGG GCACCATGCC TCAGCAGAGC 
CCTGGAGCAC AATCCTCCTG CAGCAGTGAC CACTCCCCAG CAGGCAGCGC GGCCAACACG 
GACAGCGGCG TGGAGATGGC CGGCAACGCA GGGGGCAGCA CTGAGGACTT GTCCAGCTTG 
GATGAAGGAC CTTGCGTTGC GGCTACCGGA CTCTCCACCC TTCGCCGCCT TGAGAACCTC 
AGGCTGGATC AGCTGCATCA GCTCCGGCCA ATAGGGTCCC GGGGTCTCAA ACTGCCCAGC 
TTAACCCACG CTGGCGCACC TGTGTCTCGA CGTCTGGGCC CACCGGTCTC CCTTGACCGC 
CGCAGCAGCA GCTCCAGCAG CATTAGCTCC GCGTACACAG TCAGCCGCCG GTCCTCGCTG 
GCATCCCCGT TCCCGCCGGG ATCCCCACCA GAGAACGGGG CGTCGTCACT ACCTGGCCTC 
ACACCTGCCC AGCACTACAT GCTCCGGGCA AGATATGCTT CAGCCAGGGG GAGTGGCACC 
CCACCCACTG CAGCACACAG CCTGGATCGG ATCGGGGGTC TTCCTGCACC TCCTTGGAGA 
AGCCGAGCTG AGTACCCAGG ATACAACCCC AATGCAGGGG TCACGAGGAG GGCCAGTGAC 
CCAGCCCGGG CTGCTGACCA CCCGGCTCCA GCTAGAGTAC AACGGTTCAA GAGCTTGGGG 
TGTGTACACA CGCCTCCTAG TGTGGCAACA GGACGGAACT TCGATCCCCA CCTCCCTACC 
TCTGTCTATT CGCCACAGCC CCCCAGCATC ACCGAAAATG TTGCTATGGA TACTAGAGGG 
CTACAGGGGG AGCTAGAGGT TGGAACTTCC ATGATGGGCA ATGATCTGAA CCCATACATG 
GACTTTTCTT CTGCTGACAC TCTGGGATAT GGGGGGCCTG AGGGGACGGC GGCTGAGTCT 
TATGGATCTA AGGGTCCAGG TTCCCTGCCT CTTGGGCCTG GTCCACCAAC CAACTATGGC 
CCTGGCCACT GTGCCCAGCA GGTCTCCTAT CCTGATCCCA CCCCAGAAAC ATGGGGTGAG 
TTTCCTTCAC ATGCTGGGGT GTACCCTGGC ACTAAGGCTC TAGGTACAGC CTATAACCAG 
TGTCCTCGAC TTGAGCATTA TGGACAAGTG CAAGTAAAAC CAGAACAAGG GTGCCCAGTG 
GGGTCTGACT CCACCGGGTT GGCACCCTGC CTCAATGCCC ACCCCAGTGA AGGGTCCCCA 
GACCCACAGC CTCTGTTTTC ACATCACTCC CAGCTCCCTC CACCCCAGTA TCTCCAGTCA 
GGTCCCTACC CTCAGCCTCC CCATGACTGT CTCTCGTTAG AATCCAGGCC TGGCCTCAAT 
TTCAACCCCT CCTCTCATTC CACAGGACAG CTCAAAGCTC AGCTCCTGTG TAATTACGTT 
CAGTCGCAGC AGGAATTGTT GTGGGAGGGC AGAAGCCGGG GAGGGATCCC CAACCAGGAA 
CTTCCATATC AGAGCCCCAA GTTTCTGGGA GGTTCTCAAG TTAGTCAGAG CCCCACCAAG 
GTCCCAGCAG CAGCAGCAGC AGCTACGTTT GGATCTGGCT TTGCATCACC TGCTTTGGCC 
AATCACAAAT CAGTCTCCTA TCCTGCCCCT TCACCGTGCC ATGAAACTTT CACCATGGGA 
GTAAACAGGC CTCCCCATAG GCCAGCAGCA CCACCCCGGC TTCTGCCCCC GCTACCACCT 
TGCTATGGGC CCCTCAAGGT GGGAGATGCC AACCCCAGCT GTGGGCATCC TGAAGTGGGC 
AGGTTAGGAG CAGGCCCTGC CTTGTACCCT CCCCCTGAAG GGCAGGTGTG TAACCCTCTG 
GACTCTCTTG ACCTGGACAA CACTCAGCTG GACTTTGTGG CTATCCTAGA TGAAGCTCAA 
GGGCTGAGCC CTCCTCCTTC CCACGAGCAA GGGGACAGCT CTAAAAACAC CCCACCTCCC 
TCCGGGCCCC CCAACATGGC AGTCGGTAAC ATGAGTGTCT TACTGGGGTC TCTGCCTGGA 
GAGACGCAGT TCCTCAACTC TAGTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli1 ( XM_006241442.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241504.1 Rattus norvegicus GLI family zinc finger 1 (Gli1), transcript variant X1, mRNA.

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CloneID	ORa12602
Clone ID Related Accession (Same CDS sequence)	XM_017594631.1 , NM_001191910.1 , XM_006241442.3 , XM_008765375.2 , XM_006241443.3
Accession Version	XM_006241442.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein GLI1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAATC CAATGACTTC ACCACAAGTC AATAGCTATG GTGAGCCATG CTGTCTCCGA 
CCCCTCCCCA GCCAAGGGGT CCCCAGCATG GGAACAGAAG GACTTTCTGG TCTGCCCTTT 
TGCCACCAAG CCAATTTTAT GTCAGGGTCC CAGGGTTATG GGTCTGCCAG AGAGACCAGC 
AGCTGCACTG AGGGGTCACT CTTTCCTCCT CCTCGGAGTT CAGTCAAACT GACTAAGAAG 
CGGGCTCTTT CCATCTCGCC TCTCTCTGAC GCCAGCCTCG ACCTGCAAAC CGTAATCCGT 
ACCTCACCCA GCTCCCTCGT GGCTTTCATC AACTCTCGTT GTACATCTCC AGGCGGTTCC 
TACGGCCATC TCTCCATTGG CACCATGAGC CCTTCTTTAG GATTCCCACC TCAGATGAGT 
CACCAGAAAG GAACTTCACC TCCCTATGGA GTCCAGCCCT GTGTTCCACA TGATTCTACT 
CAGGGTTCAA TGATGCTTCA CCCCCAGTCC CGAGGACCAC GTGCAACCTG CCAGCTGAAG 
TCAGAGCTGG ACATCATGGT TGGCAAGTGC CCAGAGGAAC CTTTGGAAGG GGACATGTCT 
AGCCCCAACT CCACGAGCAC ACAGGATCAC CTGCTGGGGA TGCTGGATGG ACGGGAGGAC 
CTGGAGAGAG AGGAGAAACC TGAGCCTGAG TCTGTGTATG AGACGGACTG CCGCTGGGAT 
GGTTGCAGCC AGGAGTTCGA TTCCCAAGAA CAGCTGGTGC ACCACATCAA CAGTGAGCAC 
ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGAG GCTGCTCCAG GGAGCTGAGG 
CCCTTCAAGG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA 
CACAAGTGCA CGTTTGAAGG CTGTCGGAAG TCCTATTCAC GCCTCGAAAA CCTGAAGACA 
CACCTTCGGT CGCACACCGG TGAGAAGCCT TATATGTGTG AGCAGGAAGG CTGCAGCAAG 
GCCTTCAGCA ACGCCAGTGA CCGTGCCAAG CACCAGAATA GGACCCACTC CAATGAGAAG 
CCATATGTGT GCAAACTCCC CGGCTGCACC AAGCGCTACA CAGATCCCAG CTCGCTCCGC 
AAACATGTCA AGACTGTGCA CGGTCCGGAT GCCCACGTGA CCAAACGACA TCGAGGGGAT 
GGCCCCTTAC CACGGGCACA GCCCCTCTCC ACAGTGGAGC CCAAGCGAGA AAGGGAAGGA 
GGATCCGGCA GGGAAGAGAG CAGACTGACT GTGCCGGAGG GCACCATGCC TCAGCAGAGC 
CCTGGAGCAC AATCCTCCTG CAGCAGTGAC CACTCCCCAG CAGGCAGCGC GGCCAACACG 
GACAGCGGCG TGGAGATGGC CGGCAACGCA GGGGGCAGCA CTGAGGACTT GTCCAGCTTG 
GATGAAGGAC CTTGCGTTGC GGCTACCGGA CTCTCCACCC TTCGCCGCCT TGAGAACCTC 
AGGCTGGATC AGCTGCATCA GCTCCGGCCA ATAGGGTCCC GGGGTCTCAA ACTGCCCAGC 
TTAACCCACG CTGGCGCACC TGTGTCTCGA CGTCTGGGCC CACCGGTCTC CCTTGACCGC 
CGCAGCAGCA GCTCCAGCAG CATTAGCTCC GCGTACACAG TCAGCCGCCG GTCCTCGCTG 
GCATCCCCGT TCCCGCCGGG ATCCCCACCA GAGAACGGGG CGTCGTCACT ACCTGGCCTC 
ACACCTGCCC AGCACTACAT GCTCCGGGCA AGATATGCTT CAGCCAGGGG GAGTGGCACC 
CCACCCACTG CAGCACACAG CCTGGATCGG ATCGGGGGTC TTCCTGCACC TCCTTGGAGA 
AGCCGAGCTG AGTACCCAGG ATACAACCCC AATGCAGGGG TCACGAGGAG GGCCAGTGAC 
CCAGCCCGGG CTGCTGACCA CCCGGCTCCA GCTAGAGTAC AACGGTTCAA GAGCTTGGGG 
TGTGTACACA CGCCTCCTAG TGTGGCAACA GGACGGAACT TCGATCCCCA CCTCCCTACC 
TCTGTCTATT CGCCACAGCC CCCCAGCATC ACCGAAAATG TTGCTATGGA TACTAGAGGG 
CTACAGGGGG AGCTAGAGGT TGGAACTTCC ATGATGGGCA ATGATCTGAA CCCATACATG 
GACTTTTCTT CTGCTGACAC TCTGGGATAT GGGGGGCCTG AGGGGACGGC GGCTGAGTCT 
TATGGATCTA AGGGTCCAGG TTCCCTGCCT CTTGGGCCTG GTCCACCAAC CAACTATGGC 
CCTGGCCACT GTGCCCAGCA GGTCTCCTAT CCTGATCCCA CCCCAGAAAC ATGGGGTGAG 
TTTCCTTCAC ATGCTGGGGT GTACCCTGGC ACTAAGGCTC TAGGTACAGC CTATAACCAG 
TGTCCTCGAC TTGAGCATTA TGGACAAGTG CAAGTAAAAC CAGAACAAGG GTGCCCAGTG 
GGGTCTGACT CCACCGGGTT GGCACCCTGC CTCAATGCCC ACCCCAGTGA AGGGTCCCCA 
GACCCACAGC CTCTGTTTTC ACATCACTCC CAGCTCCCTC CACCCCAGTA TCTCCAGTCA 
GGTCCCTACC CTCAGCCTCC CCATGACTGT CTCTCGTTAG AATCCAGGCC TGGCCTCAAT 
TTCAACCCCT CCTCTCATTC CACAGGACAG CTCAAAGCTC AGCTCCTGTG TAATTACGTT 
CAGTCGCAGC AGGAATTGTT GTGGGAGGGC AGAAGCCGGG GAGGGATCCC CAACCAGGAA 
CTTCCATATC AGAGCCCCAA GTTTCTGGGA GGTTCTCAAG TTAGTCAGAG CCCCACCAAG 
GTCCCAGCAG CAGCAGCAGC AGCTACGTTT GGATCTGGCT TTGCATCACC TGCTTTGGCC 
AATCACAAAT CAGTCTCCTA TCCTGCCCCT TCACCGTGCC ATGAAACTTT CACCATGGGA 
GTAAACAGGC CTCCCCATAG GCCAGCAGCA CCACCCCGGC TTCTGCCCCC GCTACCACCT 
TGCTATGGGC CCCTCAAGGT GGGAGATGCC AACCCCAGCT GTGGGCATCC TGAAGTGGGC 
AGGTTAGGAG CAGGCCCTGC CTTGTACCCT CCCCCTGAAG GGCAGGTGTG TAACCCTCTG 
GACTCTCTTG ACCTGGACAA CACTCAGCTG GACTTTGTGG CTATCCTAGA TGAAGCTCAA 
GGGCTGAGCC CTCCTCCTTC CCACGAGCAA GGGGACAGCT CTAAAAACAC CCCACCTCCC 
TCCGGGCCCC CCAACATGGC AGTCGGTAAC ATGAGTGTCT TACTGGGGTC TCTGCCTGGA 
GAGACGCAGT TCCTCAACTC TAGTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241504.1
CDS	274..3603
Translation	MFNPMTSPQVNSYGEPCCLRPLPSQGVPSMGTEGLSGLPFCHQANFMSGSQGYGSARETSSCTEGSLFPPPRSSVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPPQMSHQKGTSPPYGVQPCVPHDSTQGSMMLHPQSRGPRATCQLKSELDIMVGKCPEEPLEGDMSSPNSTSTQDHLLGMLDGREDLEREEKPEPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEQEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAQPLSTVEPKREREGGSGREESRLTVPEGTMPQQSPGAQSSCSSDHSPAGSAANTDSGVEMAGNAGGSTEDLSSLDEGPCVAATGLSTLRRLENLRLDQLHQLRPIGSRGLKLPSLTHAGAPVSRRLGPPVSLDRRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSLPGLTPAQHYMLRARYASARGSGTPPTAAHSLDRIGGLPAPPWRSRAEYPGYNPNAGVTRRASDPARAADHPAPARVQRFKSLGCVHTPPSVATGRNFDPHLPTSVYSPQPPSITENVAMDTRGLQGELEVGTSMMGNDLNPYMDFSSADTLGYGGPEGTAAESYGSKGPGSLPLGPGPPTNYGPGHCAQQVSYPDPTPETWGEFPSHAGVYPGTKALGTAYNQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGSPDPQPLFSHHSQLPPPQYLQSGPYPQPPHDCLSLESRPGLNFNPSSHSTGQLKAQLLCNYVQSQQELLWEGRSRGGIPNQELPYQSPKFLGGSQVSQSPTKVPAAAAAATFGSGFASPALANHKSVSYPAPSPCHETFTMGVNRPPHRPAAPPRLLPPLPPCYGPLKVGDANPSCGHPEVGRLGAGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEAQGLSPPPSHEQGDSSKNTPPPSGPPNMAVGNMSVLLGSLPGETQFLNSSA

Target ORF information:

RefSeq Version	XM_006241442.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GLI family zinc finger 1 (Gli1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241442.3

ORF Insert Sequence:

ATGTTCAATC CAATGACTTC ACCACAAGTC AATAGCTATG GTGAGCCATG CTGTCTCCGA 
CCCCTCCCCA GCCAAGGGGT CCCCAGCATG GGAACAGAAG GACTTTCTGG TCTGCCCTTT 
TGCCACCAAG CCAATTTTAT GTCAGGGTCC CAGGGTTATG GGTCTGCCAG AGAGACCAGC 
AGCTGCACTG AGGGGTCACT CTTTCCTCCT CCTCGGAGTT CAGTCAAACT GACTAAGAAG 
CGGGCTCTTT CCATCTCGCC TCTCTCTGAC GCCAGCCTCG ACCTGCAAAC CGTAATCCGT 
ACCTCACCCA GCTCCCTCGT GGCTTTCATC AACTCTCGTT GTACATCTCC AGGCGGTTCC 
TACGGCCATC TCTCCATTGG CACCATGAGC CCTTCTTTAG GATTCCCACC TCAGATGAGT 
CACCAGAAAG GAACTTCACC TCCCTATGGA GTCCAGCCCT GTGTTCCACA TGATTCTACT 
CAGGGTTCAA TGATGCTTCA CCCCCAGTCC CGAGGACCAC GTGCAACCTG CCAGCTGAAG 
TCAGAGCTGG ACATCATGGT TGGCAAGTGC CCAGAGGAAC CTTTGGAAGG GGACATGTCT 
AGCCCCAACT CCACGAGCAC ACAGGATCAC CTGCTGGGGA TGCTGGATGG ACGGGAGGAC 
CTGGAGAGAG AGGAGAAACC TGAGCCTGAG TCTGTGTATG AGACGGACTG CCGCTGGGAT 
GGTTGCAGCC AGGAGTTCGA TTCCCAAGAA CAGCTGGTGC ACCACATCAA CAGTGAGCAC 
ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGAG GCTGCTCCAG GGAGCTGAGG 
CCCTTCAAGG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA 
CACAAGTGCA CGTTTGAAGG CTGTCGGAAG TCCTATTCAC GCCTCGAAAA CCTGAAGACA 
CACCTTCGGT CGCACACCGG TGAGAAGCCT TATATGTGTG AGCAGGAAGG CTGCAGCAAG 
GCCTTCAGCA ACGCCAGTGA CCGTGCCAAG CACCAGAATA GGACCCACTC CAATGAGAAG 
CCATATGTGT GCAAACTCCC CGGCTGCACC AAGCGCTACA CAGATCCCAG CTCGCTCCGC 
AAACATGTCA AGACTGTGCA CGGTCCGGAT GCCCACGTGA CCAAACGACA TCGAGGGGAT 
GGCCCCTTAC CACGGGCACA GCCCCTCTCC ACAGTGGAGC CCAAGCGAGA AAGGGAAGGA 
GGATCCGGCA GGGAAGAGAG CAGACTGACT GTGCCGGAGG GCACCATGCC TCAGCAGAGC 
CCTGGAGCAC AATCCTCCTG CAGCAGTGAC CACTCCCCAG CAGGCAGCGC GGCCAACACG 
GACAGCGGCG TGGAGATGGC CGGCAACGCA GGGGGCAGCA CTGAGGACTT GTCCAGCTTG 
GATGAAGGAC CTTGCGTTGC GGCTACCGGA CTCTCCACCC TTCGCCGCCT TGAGAACCTC 
AGGCTGGATC AGCTGCATCA GCTCCGGCCA ATAGGGTCCC GGGGTCTCAA ACTGCCCAGC 
TTAACCCACG CTGGCGCACC TGTGTCTCGA CGTCTGGGCC CACCGGTCTC CCTTGACCGC 
CGCAGCAGCA GCTCCAGCAG CATTAGCTCC GCGTACACAG TCAGCCGCCG GTCCTCGCTG 
GCATCCCCGT TCCCGCCGGG ATCCCCACCA GAGAACGGGG CGTCGTCACT ACCTGGCCTC 
ACACCTGCCC AGCACTACAT GCTCCGGGCA AGATATGCTT CAGCCAGGGG GAGTGGCACC 
CCACCCACTG CAGCACACAG CCTGGATCGG ATCGGGGGTC TTCCTGCACC TCCTTGGAGA 
AGCCGAGCTG AGTACCCAGG ATACAACCCC AATGCAGGGG TCACGAGGAG GGCCAGTGAC 
CCAGCCCGGG CTGCTGACCA CCCGGCTCCA GCTAGAGTAC AACGGTTCAA GAGCTTGGGG 
TGTGTACACA CGCCTCCTAG TGTGGCAACA GGACGGAACT TCGATCCCCA CCTCCCTACC 
TCTGTCTATT CGCCACAGCC CCCCAGCATC ACCGAAAATG TTGCTATGGA TACTAGAGGG 
CTACAGGGGG AGCTAGAGGT TGGAACTTCC ATGATGGGCA ATGATCTGAA CCCATACATG 
GACTTTTCTT CTGCTGACAC TCTGGGATAT GGGGGGCCTG AGGGGACGGC GGCTGAGTCT 
TATGGATCTA AGGGTCCAGG TTCCCTGCCT CTTGGGCCTG GTCCACCAAC CAACTATGGC 
CCTGGCCACT GTGCCCAGCA GGTCTCCTAT CCTGATCCCA CCCCAGAAAC ATGGGGTGAG 
TTTCCTTCAC ATGCTGGGGT GTACCCTGGC ACTAAGGCTC TAGGTACAGC CTATAACCAG 
TGTCCTCGAC TTGAGCATTA TGGACAAGTG CAAGTAAAAC CAGAACAAGG GTGCCCAGTG 
GGGTCTGACT CCACCGGGTT GGCACCCTGC CTCAATGCCC ACCCCAGTGA AGGGTCCCCA 
GACCCACAGC CTCTGTTTTC ACATCACTCC CAGCTCCCTC CACCCCAGTA TCTCCAGTCA 
GGTCCCTACC CTCAGCCTCC CCATGACTGT CTCTCGTTAG AATCCAGGCC TGGCCTCAAT 
TTCAACCCCT CCTCTCATTC CACAGGACAG CTCAAAGCTC AGCTCCTGTG TAATTACGTT 
CAGTCGCAGC AGGAATTGTT GTGGGAGGGC AGAAGCCGGG GAGGGATCCC CAACCAGGAA 
CTTCCATATC AGAGCCCCAA GTTTCTGGGA GGTTCTCAAG TTAGTCAGAG CCCCACCAAG 
GTCCCAGCAG CAGCAGCAGC AGCTACGTTT GGATCTGGCT TTGCATCACC TGCTTTGGCC 
AATCACAAAT CAGTCTCCTA TCCTGCCCCT TCACCGTGCC ATGAAACTTT CACCATGGGA 
GTAAACAGGC CTCCCCATAG GCCAGCAGCA CCACCCCGGC TTCTGCCCCC GCTACCACCT 
TGCTATGGGC CCCTCAAGGT GGGAGATGCC AACCCCAGCT GTGGGCATCC TGAAGTGGGC 
AGGTTAGGAG CAGGCCCTGC CTTGTACCCT CCCCCTGAAG GGCAGGTGTG TAACCCTCTG 
GACTCTCTTG ACCTGGACAA CACTCAGCTG GACTTTGTGG CTATCCTAGA TGAAGCTCAA 
GGGCTGAGCC CTCCTCCTTC CCACGAGCAA GGGGACAGCT CTAAAAACAC CCCACCTCCC 
TCCGGGCCCC CCAACATGGC AGTCGGTAAC ATGAGTGTCT TACTGGGGTC TCTGCCTGGA 
GAGACGCAGT TCCTCAACTC TAGTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli1 ( XM_008765375.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763597.1 Rattus norvegicus GLI family zinc finger 1 (Gli1), transcript variant X3, mRNA.

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CloneID	ORa12602
Clone ID Related Accession (Same CDS sequence)	XM_017594631.1 , NM_001191910.1 , XM_006241442.3 , XM_008765375.2 , XM_006241443.3
Accession Version	XM_008765375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein GLI1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAATC CAATGACTTC ACCACAAGTC AATAGCTATG GTGAGCCATG CTGTCTCCGA 
CCCCTCCCCA GCCAAGGGGT CCCCAGCATG GGAACAGAAG GACTTTCTGG TCTGCCCTTT 
TGCCACCAAG CCAATTTTAT GTCAGGGTCC CAGGGTTATG GGTCTGCCAG AGAGACCAGC 
AGCTGCACTG AGGGGTCACT CTTTCCTCCT CCTCGGAGTT CAGTCAAACT GACTAAGAAG 
CGGGCTCTTT CCATCTCGCC TCTCTCTGAC GCCAGCCTCG ACCTGCAAAC CGTAATCCGT 
ACCTCACCCA GCTCCCTCGT GGCTTTCATC AACTCTCGTT GTACATCTCC AGGCGGTTCC 
TACGGCCATC TCTCCATTGG CACCATGAGC CCTTCTTTAG GATTCCCACC TCAGATGAGT 
CACCAGAAAG GAACTTCACC TCCCTATGGA GTCCAGCCCT GTGTTCCACA TGATTCTACT 
CAGGGTTCAA TGATGCTTCA CCCCCAGTCC CGAGGACCAC GTGCAACCTG CCAGCTGAAG 
TCAGAGCTGG ACATCATGGT TGGCAAGTGC CCAGAGGAAC CTTTGGAAGG GGACATGTCT 
AGCCCCAACT CCACGAGCAC ACAGGATCAC CTGCTGGGGA TGCTGGATGG ACGGGAGGAC 
CTGGAGAGAG AGGAGAAACC TGAGCCTGAG TCTGTGTATG AGACGGACTG CCGCTGGGAT 
GGTTGCAGCC AGGAGTTCGA TTCCCAAGAA CAGCTGGTGC ACCACATCAA CAGTGAGCAC 
ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGAG GCTGCTCCAG GGAGCTGAGG 
CCCTTCAAGG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA 
CACAAGTGCA CGTTTGAAGG CTGTCGGAAG TCCTATTCAC GCCTCGAAAA CCTGAAGACA 
CACCTTCGGT CGCACACCGG TGAGAAGCCT TATATGTGTG AGCAGGAAGG CTGCAGCAAG 
GCCTTCAGCA ACGCCAGTGA CCGTGCCAAG CACCAGAATA GGACCCACTC CAATGAGAAG 
CCATATGTGT GCAAACTCCC CGGCTGCACC AAGCGCTACA CAGATCCCAG CTCGCTCCGC 
AAACATGTCA AGACTGTGCA CGGTCCGGAT GCCCACGTGA CCAAACGACA TCGAGGGGAT 
GGCCCCTTAC CACGGGCACA GCCCCTCTCC ACAGTGGAGC CCAAGCGAGA AAGGGAAGGA 
GGATCCGGCA GGGAAGAGAG CAGACTGACT GTGCCGGAGG GCACCATGCC TCAGCAGAGC 
CCTGGAGCAC AATCCTCCTG CAGCAGTGAC CACTCCCCAG CAGGCAGCGC GGCCAACACG 
GACAGCGGCG TGGAGATGGC CGGCAACGCA GGGGGCAGCA CTGAGGACTT GTCCAGCTTG 
GATGAAGGAC CTTGCGTTGC GGCTACCGGA CTCTCCACCC TTCGCCGCCT TGAGAACCTC 
AGGCTGGATC AGCTGCATCA GCTCCGGCCA ATAGGGTCCC GGGGTCTCAA ACTGCCCAGC 
TTAACCCACG CTGGCGCACC TGTGTCTCGA CGTCTGGGCC CACCGGTCTC CCTTGACCGC 
CGCAGCAGCA GCTCCAGCAG CATTAGCTCC GCGTACACAG TCAGCCGCCG GTCCTCGCTG 
GCATCCCCGT TCCCGCCGGG ATCCCCACCA GAGAACGGGG CGTCGTCACT ACCTGGCCTC 
ACACCTGCCC AGCACTACAT GCTCCGGGCA AGATATGCTT CAGCCAGGGG GAGTGGCACC 
CCACCCACTG CAGCACACAG CCTGGATCGG ATCGGGGGTC TTCCTGCACC TCCTTGGAGA 
AGCCGAGCTG AGTACCCAGG ATACAACCCC AATGCAGGGG TCACGAGGAG GGCCAGTGAC 
CCAGCCCGGG CTGCTGACCA CCCGGCTCCA GCTAGAGTAC AACGGTTCAA GAGCTTGGGG 
TGTGTACACA CGCCTCCTAG TGTGGCAACA GGACGGAACT TCGATCCCCA CCTCCCTACC 
TCTGTCTATT CGCCACAGCC CCCCAGCATC ACCGAAAATG TTGCTATGGA TACTAGAGGG 
CTACAGGGGG AGCTAGAGGT TGGAACTTCC ATGATGGGCA ATGATCTGAA CCCATACATG 
GACTTTTCTT CTGCTGACAC TCTGGGATAT GGGGGGCCTG AGGGGACGGC GGCTGAGTCT 
TATGGATCTA AGGGTCCAGG TTCCCTGCCT CTTGGGCCTG GTCCACCAAC CAACTATGGC 
CCTGGCCACT GTGCCCAGCA GGTCTCCTAT CCTGATCCCA CCCCAGAAAC ATGGGGTGAG 
TTTCCTTCAC ATGCTGGGGT GTACCCTGGC ACTAAGGCTC TAGGTACAGC CTATAACCAG 
TGTCCTCGAC TTGAGCATTA TGGACAAGTG CAAGTAAAAC CAGAACAAGG GTGCCCAGTG 
GGGTCTGACT CCACCGGGTT GGCACCCTGC CTCAATGCCC ACCCCAGTGA AGGGTCCCCA 
GACCCACAGC CTCTGTTTTC ACATCACTCC CAGCTCCCTC CACCCCAGTA TCTCCAGTCA 
GGTCCCTACC CTCAGCCTCC CCATGACTGT CTCTCGTTAG AATCCAGGCC TGGCCTCAAT 
TTCAACCCCT CCTCTCATTC CACAGGACAG CTCAAAGCTC AGCTCCTGTG TAATTACGTT 
CAGTCGCAGC AGGAATTGTT GTGGGAGGGC AGAAGCCGGG GAGGGATCCC CAACCAGGAA 
CTTCCATATC AGAGCCCCAA GTTTCTGGGA GGTTCTCAAG TTAGTCAGAG CCCCACCAAG 
GTCCCAGCAG CAGCAGCAGC AGCTACGTTT GGATCTGGCT TTGCATCACC TGCTTTGGCC 
AATCACAAAT CAGTCTCCTA TCCTGCCCCT TCACCGTGCC ATGAAACTTT CACCATGGGA 
GTAAACAGGC CTCCCCATAG GCCAGCAGCA CCACCCCGGC TTCTGCCCCC GCTACCACCT 
TGCTATGGGC CCCTCAAGGT GGGAGATGCC AACCCCAGCT GTGGGCATCC TGAAGTGGGC 
AGGTTAGGAG CAGGCCCTGC CTTGTACCCT CCCCCTGAAG GGCAGGTGTG TAACCCTCTG 
GACTCTCTTG ACCTGGACAA CACTCAGCTG GACTTTGTGG CTATCCTAGA TGAAGCTCAA 
GGGCTGAGCC CTCCTCCTTC CCACGAGCAA GGGGACAGCT CTAAAAACAC CCCACCTCCC 
TCCGGGCCCC CCAACATGGC AGTCGGTAAC ATGAGTGTCT TACTGGGGTC TCTGCCTGGA 
GAGACGCAGT TCCTCAACTC TAGTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763597.1
CDS	222..3551
Translation	MFNPMTSPQVNSYGEPCCLRPLPSQGVPSMGTEGLSGLPFCHQANFMSGSQGYGSARETSSCTEGSLFPPPRSSVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPPQMSHQKGTSPPYGVQPCVPHDSTQGSMMLHPQSRGPRATCQLKSELDIMVGKCPEEPLEGDMSSPNSTSTQDHLLGMLDGREDLEREEKPEPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEQEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAQPLSTVEPKREREGGSGREESRLTVPEGTMPQQSPGAQSSCSSDHSPAGSAANTDSGVEMAGNAGGSTEDLSSLDEGPCVAATGLSTLRRLENLRLDQLHQLRPIGSRGLKLPSLTHAGAPVSRRLGPPVSLDRRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSLPGLTPAQHYMLRARYASARGSGTPPTAAHSLDRIGGLPAPPWRSRAEYPGYNPNAGVTRRASDPARAADHPAPARVQRFKSLGCVHTPPSVATGRNFDPHLPTSVYSPQPPSITENVAMDTRGLQGELEVGTSMMGNDLNPYMDFSSADTLGYGGPEGTAAESYGSKGPGSLPLGPGPPTNYGPGHCAQQVSYPDPTPETWGEFPSHAGVYPGTKALGTAYNQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGSPDPQPLFSHHSQLPPPQYLQSGPYPQPPHDCLSLESRPGLNFNPSSHSTGQLKAQLLCNYVQSQQELLWEGRSRGGIPNQELPYQSPKFLGGSQVSQSPTKVPAAAAAATFGSGFASPALANHKSVSYPAPSPCHETFTMGVNRPPHRPAAPPRLLPPLPPCYGPLKVGDANPSCGHPEVGRLGAGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEAQGLSPPPSHEQGDSSKNTPPPSGPPNMAVGNMSVLLGSLPGETQFLNSSA

Target ORF information:

RefSeq Version	XM_008765375.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GLI family zinc finger 1 (Gli1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765375.2

ORF Insert Sequence:

ATGTTCAATC CAATGACTTC ACCACAAGTC AATAGCTATG GTGAGCCATG CTGTCTCCGA 
CCCCTCCCCA GCCAAGGGGT CCCCAGCATG GGAACAGAAG GACTTTCTGG TCTGCCCTTT 
TGCCACCAAG CCAATTTTAT GTCAGGGTCC CAGGGTTATG GGTCTGCCAG AGAGACCAGC 
AGCTGCACTG AGGGGTCACT CTTTCCTCCT CCTCGGAGTT CAGTCAAACT GACTAAGAAG 
CGGGCTCTTT CCATCTCGCC TCTCTCTGAC GCCAGCCTCG ACCTGCAAAC CGTAATCCGT 
ACCTCACCCA GCTCCCTCGT GGCTTTCATC AACTCTCGTT GTACATCTCC AGGCGGTTCC 
TACGGCCATC TCTCCATTGG CACCATGAGC CCTTCTTTAG GATTCCCACC TCAGATGAGT 
CACCAGAAAG GAACTTCACC TCCCTATGGA GTCCAGCCCT GTGTTCCACA TGATTCTACT 
CAGGGTTCAA TGATGCTTCA CCCCCAGTCC CGAGGACCAC GTGCAACCTG CCAGCTGAAG 
TCAGAGCTGG ACATCATGGT TGGCAAGTGC CCAGAGGAAC CTTTGGAAGG GGACATGTCT 
AGCCCCAACT CCACGAGCAC ACAGGATCAC CTGCTGGGGA TGCTGGATGG ACGGGAGGAC 
CTGGAGAGAG AGGAGAAACC TGAGCCTGAG TCTGTGTATG AGACGGACTG CCGCTGGGAT 
GGTTGCAGCC AGGAGTTCGA TTCCCAAGAA CAGCTGGTGC ACCACATCAA CAGTGAGCAC 
ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGAG GCTGCTCCAG GGAGCTGAGG 
CCCTTCAAGG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA 
CACAAGTGCA CGTTTGAAGG CTGTCGGAAG TCCTATTCAC GCCTCGAAAA CCTGAAGACA 
CACCTTCGGT CGCACACCGG TGAGAAGCCT TATATGTGTG AGCAGGAAGG CTGCAGCAAG 
GCCTTCAGCA ACGCCAGTGA CCGTGCCAAG CACCAGAATA GGACCCACTC CAATGAGAAG 
CCATATGTGT GCAAACTCCC CGGCTGCACC AAGCGCTACA CAGATCCCAG CTCGCTCCGC 
AAACATGTCA AGACTGTGCA CGGTCCGGAT GCCCACGTGA CCAAACGACA TCGAGGGGAT 
GGCCCCTTAC CACGGGCACA GCCCCTCTCC ACAGTGGAGC CCAAGCGAGA AAGGGAAGGA 
GGATCCGGCA GGGAAGAGAG CAGACTGACT GTGCCGGAGG GCACCATGCC TCAGCAGAGC 
CCTGGAGCAC AATCCTCCTG CAGCAGTGAC CACTCCCCAG CAGGCAGCGC GGCCAACACG 
GACAGCGGCG TGGAGATGGC CGGCAACGCA GGGGGCAGCA CTGAGGACTT GTCCAGCTTG 
GATGAAGGAC CTTGCGTTGC GGCTACCGGA CTCTCCACCC TTCGCCGCCT TGAGAACCTC 
AGGCTGGATC AGCTGCATCA GCTCCGGCCA ATAGGGTCCC GGGGTCTCAA ACTGCCCAGC 
TTAACCCACG CTGGCGCACC TGTGTCTCGA CGTCTGGGCC CACCGGTCTC CCTTGACCGC 
CGCAGCAGCA GCTCCAGCAG CATTAGCTCC GCGTACACAG TCAGCCGCCG GTCCTCGCTG 
GCATCCCCGT TCCCGCCGGG ATCCCCACCA GAGAACGGGG CGTCGTCACT ACCTGGCCTC 
ACACCTGCCC AGCACTACAT GCTCCGGGCA AGATATGCTT CAGCCAGGGG GAGTGGCACC 
CCACCCACTG CAGCACACAG CCTGGATCGG ATCGGGGGTC TTCCTGCACC TCCTTGGAGA 
AGCCGAGCTG AGTACCCAGG ATACAACCCC AATGCAGGGG TCACGAGGAG GGCCAGTGAC 
CCAGCCCGGG CTGCTGACCA CCCGGCTCCA GCTAGAGTAC AACGGTTCAA GAGCTTGGGG 
TGTGTACACA CGCCTCCTAG TGTGGCAACA GGACGGAACT TCGATCCCCA CCTCCCTACC 
TCTGTCTATT CGCCACAGCC CCCCAGCATC ACCGAAAATG TTGCTATGGA TACTAGAGGG 
CTACAGGGGG AGCTAGAGGT TGGAACTTCC ATGATGGGCA ATGATCTGAA CCCATACATG 
GACTTTTCTT CTGCTGACAC TCTGGGATAT GGGGGGCCTG AGGGGACGGC GGCTGAGTCT 
TATGGATCTA AGGGTCCAGG TTCCCTGCCT CTTGGGCCTG GTCCACCAAC CAACTATGGC 
CCTGGCCACT GTGCCCAGCA GGTCTCCTAT CCTGATCCCA CCCCAGAAAC ATGGGGTGAG 
TTTCCTTCAC ATGCTGGGGT GTACCCTGGC ACTAAGGCTC TAGGTACAGC CTATAACCAG 
TGTCCTCGAC TTGAGCATTA TGGACAAGTG CAAGTAAAAC CAGAACAAGG GTGCCCAGTG 
GGGTCTGACT CCACCGGGTT GGCACCCTGC CTCAATGCCC ACCCCAGTGA AGGGTCCCCA 
GACCCACAGC CTCTGTTTTC ACATCACTCC CAGCTCCCTC CACCCCAGTA TCTCCAGTCA 
GGTCCCTACC CTCAGCCTCC CCATGACTGT CTCTCGTTAG AATCCAGGCC TGGCCTCAAT 
TTCAACCCCT CCTCTCATTC CACAGGACAG CTCAAAGCTC AGCTCCTGTG TAATTACGTT 
CAGTCGCAGC AGGAATTGTT GTGGGAGGGC AGAAGCCGGG GAGGGATCCC CAACCAGGAA 
CTTCCATATC AGAGCCCCAA GTTTCTGGGA GGTTCTCAAG TTAGTCAGAG CCCCACCAAG 
GTCCCAGCAG CAGCAGCAGC AGCTACGTTT GGATCTGGCT TTGCATCACC TGCTTTGGCC 
AATCACAAAT CAGTCTCCTA TCCTGCCCCT TCACCGTGCC ATGAAACTTT CACCATGGGA 
GTAAACAGGC CTCCCCATAG GCCAGCAGCA CCACCCCGGC TTCTGCCCCC GCTACCACCT 
TGCTATGGGC CCCTCAAGGT GGGAGATGCC AACCCCAGCT GTGGGCATCC TGAAGTGGGC 
AGGTTAGGAG CAGGCCCTGC CTTGTACCCT CCCCCTGAAG GGCAGGTGTG TAACCCTCTG 
GACTCTCTTG ACCTGGACAA CACTCAGCTG GACTTTGTGG CTATCCTAGA TGAAGCTCAA 
GGGCTGAGCC CTCCTCCTTC CCACGAGCAA GGGGACAGCT CTAAAAACAC CCCACCTCCC 
TCCGGGCCCC CCAACATGGC AGTCGGTAAC ATGAGTGTCT TACTGGGGTC TCTGCCTGGA 
GAGACGCAGT TCCTCAACTC TAGTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli1 ( XM_006241443.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241505.1 Rattus norvegicus GLI family zinc finger 1 (Gli1), transcript variant X4, mRNA.

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CloneID	ORa12602
Clone ID Related Accession (Same CDS sequence)	XM_017594631.1 , NM_001191910.1 , XM_006241442.3 , XM_008765375.2 , XM_006241443.3
Accession Version	XM_006241443.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein GLI1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241443.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAATC CAATGACTTC ACCACAAGTC AATAGCTATG GTGAGCCATG CTGTCTCCGA 
CCCCTCCCCA GCCAAGGGGT CCCCAGCATG GGAACAGAAG GACTTTCTGG TCTGCCCTTT 
TGCCACCAAG CCAATTTTAT GTCAGGGTCC CAGGGTTATG GGTCTGCCAG AGAGACCAGC 
AGCTGCACTG AGGGGTCACT CTTTCCTCCT CCTCGGAGTT CAGTCAAACT GACTAAGAAG 
CGGGCTCTTT CCATCTCGCC TCTCTCTGAC GCCAGCCTCG ACCTGCAAAC CGTAATCCGT 
ACCTCACCCA GCTCCCTCGT GGCTTTCATC AACTCTCGTT GTACATCTCC AGGCGGTTCC 
TACGGCCATC TCTCCATTGG CACCATGAGC CCTTCTTTAG GATTCCCACC TCAGATGAGT 
CACCAGAAAG GAACTTCACC TCCCTATGGA GTCCAGCCCT GTGTTCCACA TGATTCTACT 
CAGGGTTCAA TGATGCTTCA CCCCCAGTCC CGAGGACCAC GTGCAACCTG CCAGCTGAAG 
TCAGAGCTGG ACATCATGGT TGGCAAGTGC CCAGAGGAAC CTTTGGAAGG GGACATGTCT 
AGCCCCAACT CCACGAGCAC ACAGGATCAC CTGCTGGGGA TGCTGGATGG ACGGGAGGAC 
CTGGAGAGAG AGGAGAAACC TGAGCCTGAG TCTGTGTATG AGACGGACTG CCGCTGGGAT 
GGTTGCAGCC AGGAGTTCGA TTCCCAAGAA CAGCTGGTGC ACCACATCAA CAGTGAGCAC 
ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGAG GCTGCTCCAG GGAGCTGAGG 
CCCTTCAAGG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA 
CACAAGTGCA CGTTTGAAGG CTGTCGGAAG TCCTATTCAC GCCTCGAAAA CCTGAAGACA 
CACCTTCGGT CGCACACCGG TGAGAAGCCT TATATGTGTG AGCAGGAAGG CTGCAGCAAG 
GCCTTCAGCA ACGCCAGTGA CCGTGCCAAG CACCAGAATA GGACCCACTC CAATGAGAAG 
CCATATGTGT GCAAACTCCC CGGCTGCACC AAGCGCTACA CAGATCCCAG CTCGCTCCGC 
AAACATGTCA AGACTGTGCA CGGTCCGGAT GCCCACGTGA CCAAACGACA TCGAGGGGAT 
GGCCCCTTAC CACGGGCACA GCCCCTCTCC ACAGTGGAGC CCAAGCGAGA AAGGGAAGGA 
GGATCCGGCA GGGAAGAGAG CAGACTGACT GTGCCGGAGG GCACCATGCC TCAGCAGAGC 
CCTGGAGCAC AATCCTCCTG CAGCAGTGAC CACTCCCCAG CAGGCAGCGC GGCCAACACG 
GACAGCGGCG TGGAGATGGC CGGCAACGCA GGGGGCAGCA CTGAGGACTT GTCCAGCTTG 
GATGAAGGAC CTTGCGTTGC GGCTACCGGA CTCTCCACCC TTCGCCGCCT TGAGAACCTC 
AGGCTGGATC AGCTGCATCA GCTCCGGCCA ATAGGGTCCC GGGGTCTCAA ACTGCCCAGC 
TTAACCCACG CTGGCGCACC TGTGTCTCGA CGTCTGGGCC CACCGGTCTC CCTTGACCGC 
CGCAGCAGCA GCTCCAGCAG CATTAGCTCC GCGTACACAG TCAGCCGCCG GTCCTCGCTG 
GCATCCCCGT TCCCGCCGGG ATCCCCACCA GAGAACGGGG CGTCGTCACT ACCTGGCCTC 
ACACCTGCCC AGCACTACAT GCTCCGGGCA AGATATGCTT CAGCCAGGGG GAGTGGCACC 
CCACCCACTG CAGCACACAG CCTGGATCGG ATCGGGGGTC TTCCTGCACC TCCTTGGAGA 
AGCCGAGCTG AGTACCCAGG ATACAACCCC AATGCAGGGG TCACGAGGAG GGCCAGTGAC 
CCAGCCCGGG CTGCTGACCA CCCGGCTCCA GCTAGAGTAC AACGGTTCAA GAGCTTGGGG 
TGTGTACACA CGCCTCCTAG TGTGGCAACA GGACGGAACT TCGATCCCCA CCTCCCTACC 
TCTGTCTATT CGCCACAGCC CCCCAGCATC ACCGAAAATG TTGCTATGGA TACTAGAGGG 
CTACAGGGGG AGCTAGAGGT TGGAACTTCC ATGATGGGCA ATGATCTGAA CCCATACATG 
GACTTTTCTT CTGCTGACAC TCTGGGATAT GGGGGGCCTG AGGGGACGGC GGCTGAGTCT 
TATGGATCTA AGGGTCCAGG TTCCCTGCCT CTTGGGCCTG GTCCACCAAC CAACTATGGC 
CCTGGCCACT GTGCCCAGCA GGTCTCCTAT CCTGATCCCA CCCCAGAAAC ATGGGGTGAG 
TTTCCTTCAC ATGCTGGGGT GTACCCTGGC ACTAAGGCTC TAGGTACAGC CTATAACCAG 
TGTCCTCGAC TTGAGCATTA TGGACAAGTG CAAGTAAAAC CAGAACAAGG GTGCCCAGTG 
GGGTCTGACT CCACCGGGTT GGCACCCTGC CTCAATGCCC ACCCCAGTGA AGGGTCCCCA 
GACCCACAGC CTCTGTTTTC ACATCACTCC CAGCTCCCTC CACCCCAGTA TCTCCAGTCA 
GGTCCCTACC CTCAGCCTCC CCATGACTGT CTCTCGTTAG AATCCAGGCC TGGCCTCAAT 
TTCAACCCCT CCTCTCATTC CACAGGACAG CTCAAAGCTC AGCTCCTGTG TAATTACGTT 
CAGTCGCAGC AGGAATTGTT GTGGGAGGGC AGAAGCCGGG GAGGGATCCC CAACCAGGAA 
CTTCCATATC AGAGCCCCAA GTTTCTGGGA GGTTCTCAAG TTAGTCAGAG CCCCACCAAG 
GTCCCAGCAG CAGCAGCAGC AGCTACGTTT GGATCTGGCT TTGCATCACC TGCTTTGGCC 
AATCACAAAT CAGTCTCCTA TCCTGCCCCT TCACCGTGCC ATGAAACTTT CACCATGGGA 
GTAAACAGGC CTCCCCATAG GCCAGCAGCA CCACCCCGGC TTCTGCCCCC GCTACCACCT 
TGCTATGGGC CCCTCAAGGT GGGAGATGCC AACCCCAGCT GTGGGCATCC TGAAGTGGGC 
AGGTTAGGAG CAGGCCCTGC CTTGTACCCT CCCCCTGAAG GGCAGGTGTG TAACCCTCTG 
GACTCTCTTG ACCTGGACAA CACTCAGCTG GACTTTGTGG CTATCCTAGA TGAAGCTCAA 
GGGCTGAGCC CTCCTCCTTC CCACGAGCAA GGGGACAGCT CTAAAAACAC CCCACCTCCC 
TCCGGGCCCC CCAACATGGC AGTCGGTAAC ATGAGTGTCT TACTGGGGTC TCTGCCTGGA 
GAGACGCAGT TCCTCAACTC TAGTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241505.1
CDS	157..3486
Translation	MFNPMTSPQVNSYGEPCCLRPLPSQGVPSMGTEGLSGLPFCHQANFMSGSQGYGSARETSSCTEGSLFPPPRSSVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPPQMSHQKGTSPPYGVQPCVPHDSTQGSMMLHPQSRGPRATCQLKSELDIMVGKCPEEPLEGDMSSPNSTSTQDHLLGMLDGREDLEREEKPEPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEQEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAQPLSTVEPKREREGGSGREESRLTVPEGTMPQQSPGAQSSCSSDHSPAGSAANTDSGVEMAGNAGGSTEDLSSLDEGPCVAATGLSTLRRLENLRLDQLHQLRPIGSRGLKLPSLTHAGAPVSRRLGPPVSLDRRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSLPGLTPAQHYMLRARYASARGSGTPPTAAHSLDRIGGLPAPPWRSRAEYPGYNPNAGVTRRASDPARAADHPAPARVQRFKSLGCVHTPPSVATGRNFDPHLPTSVYSPQPPSITENVAMDTRGLQGELEVGTSMMGNDLNPYMDFSSADTLGYGGPEGTAAESYGSKGPGSLPLGPGPPTNYGPGHCAQQVSYPDPTPETWGEFPSHAGVYPGTKALGTAYNQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGSPDPQPLFSHHSQLPPPQYLQSGPYPQPPHDCLSLESRPGLNFNPSSHSTGQLKAQLLCNYVQSQQELLWEGRSRGGIPNQELPYQSPKFLGGSQVSQSPTKVPAAAAAATFGSGFASPALANHKSVSYPAPSPCHETFTMGVNRPPHRPAAPPRLLPPLPPCYGPLKVGDANPSCGHPEVGRLGAGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEAQGLSPPPSHEQGDSSKNTPPPSGPPNMAVGNMSVLLGSLPGETQFLNSSA

Target ORF information:

RefSeq Version	XM_006241443.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GLI family zinc finger 1 (Gli1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241443.3

ORF Insert Sequence:

ATGTTCAATC CAATGACTTC ACCACAAGTC AATAGCTATG GTGAGCCATG CTGTCTCCGA 
CCCCTCCCCA GCCAAGGGGT CCCCAGCATG GGAACAGAAG GACTTTCTGG TCTGCCCTTT 
TGCCACCAAG CCAATTTTAT GTCAGGGTCC CAGGGTTATG GGTCTGCCAG AGAGACCAGC 
AGCTGCACTG AGGGGTCACT CTTTCCTCCT CCTCGGAGTT CAGTCAAACT GACTAAGAAG 
CGGGCTCTTT CCATCTCGCC TCTCTCTGAC GCCAGCCTCG ACCTGCAAAC CGTAATCCGT 
ACCTCACCCA GCTCCCTCGT GGCTTTCATC AACTCTCGTT GTACATCTCC AGGCGGTTCC 
TACGGCCATC TCTCCATTGG CACCATGAGC CCTTCTTTAG GATTCCCACC TCAGATGAGT 
CACCAGAAAG GAACTTCACC TCCCTATGGA GTCCAGCCCT GTGTTCCACA TGATTCTACT 
CAGGGTTCAA TGATGCTTCA CCCCCAGTCC CGAGGACCAC GTGCAACCTG CCAGCTGAAG 
TCAGAGCTGG ACATCATGGT TGGCAAGTGC CCAGAGGAAC CTTTGGAAGG GGACATGTCT 
AGCCCCAACT CCACGAGCAC ACAGGATCAC CTGCTGGGGA TGCTGGATGG ACGGGAGGAC 
CTGGAGAGAG AGGAGAAACC TGAGCCTGAG TCTGTGTATG AGACGGACTG CCGCTGGGAT 
GGTTGCAGCC AGGAGTTCGA TTCCCAAGAA CAGCTGGTGC ACCACATCAA CAGTGAGCAC 
ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGAG GCTGCTCCAG GGAGCTGAGG 
CCCTTCAAGG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA 
CACAAGTGCA CGTTTGAAGG CTGTCGGAAG TCCTATTCAC GCCTCGAAAA CCTGAAGACA 
CACCTTCGGT CGCACACCGG TGAGAAGCCT TATATGTGTG AGCAGGAAGG CTGCAGCAAG 
GCCTTCAGCA ACGCCAGTGA CCGTGCCAAG CACCAGAATA GGACCCACTC CAATGAGAAG 
CCATATGTGT GCAAACTCCC CGGCTGCACC AAGCGCTACA CAGATCCCAG CTCGCTCCGC 
AAACATGTCA AGACTGTGCA CGGTCCGGAT GCCCACGTGA CCAAACGACA TCGAGGGGAT 
GGCCCCTTAC CACGGGCACA GCCCCTCTCC ACAGTGGAGC CCAAGCGAGA AAGGGAAGGA 
GGATCCGGCA GGGAAGAGAG CAGACTGACT GTGCCGGAGG GCACCATGCC TCAGCAGAGC 
CCTGGAGCAC AATCCTCCTG CAGCAGTGAC CACTCCCCAG CAGGCAGCGC GGCCAACACG 
GACAGCGGCG TGGAGATGGC CGGCAACGCA GGGGGCAGCA CTGAGGACTT GTCCAGCTTG 
GATGAAGGAC CTTGCGTTGC GGCTACCGGA CTCTCCACCC TTCGCCGCCT TGAGAACCTC 
AGGCTGGATC AGCTGCATCA GCTCCGGCCA ATAGGGTCCC GGGGTCTCAA ACTGCCCAGC 
TTAACCCACG CTGGCGCACC TGTGTCTCGA CGTCTGGGCC CACCGGTCTC CCTTGACCGC 
CGCAGCAGCA GCTCCAGCAG CATTAGCTCC GCGTACACAG TCAGCCGCCG GTCCTCGCTG 
GCATCCCCGT TCCCGCCGGG ATCCCCACCA GAGAACGGGG CGTCGTCACT ACCTGGCCTC 
ACACCTGCCC AGCACTACAT GCTCCGGGCA AGATATGCTT CAGCCAGGGG GAGTGGCACC 
CCACCCACTG CAGCACACAG CCTGGATCGG ATCGGGGGTC TTCCTGCACC TCCTTGGAGA 
AGCCGAGCTG AGTACCCAGG ATACAACCCC AATGCAGGGG TCACGAGGAG GGCCAGTGAC 
CCAGCCCGGG CTGCTGACCA CCCGGCTCCA GCTAGAGTAC AACGGTTCAA GAGCTTGGGG 
TGTGTACACA CGCCTCCTAG TGTGGCAACA GGACGGAACT TCGATCCCCA CCTCCCTACC 
TCTGTCTATT CGCCACAGCC CCCCAGCATC ACCGAAAATG TTGCTATGGA TACTAGAGGG 
CTACAGGGGG AGCTAGAGGT TGGAACTTCC ATGATGGGCA ATGATCTGAA CCCATACATG 
GACTTTTCTT CTGCTGACAC TCTGGGATAT GGGGGGCCTG AGGGGACGGC GGCTGAGTCT 
TATGGATCTA AGGGTCCAGG TTCCCTGCCT CTTGGGCCTG GTCCACCAAC CAACTATGGC 
CCTGGCCACT GTGCCCAGCA GGTCTCCTAT CCTGATCCCA CCCCAGAAAC ATGGGGTGAG 
TTTCCTTCAC ATGCTGGGGT GTACCCTGGC ACTAAGGCTC TAGGTACAGC CTATAACCAG 
TGTCCTCGAC TTGAGCATTA TGGACAAGTG CAAGTAAAAC CAGAACAAGG GTGCCCAGTG 
GGGTCTGACT CCACCGGGTT GGCACCCTGC CTCAATGCCC ACCCCAGTGA AGGGTCCCCA 
GACCCACAGC CTCTGTTTTC ACATCACTCC CAGCTCCCTC CACCCCAGTA TCTCCAGTCA 
GGTCCCTACC CTCAGCCTCC CCATGACTGT CTCTCGTTAG AATCCAGGCC TGGCCTCAAT 
TTCAACCCCT CCTCTCATTC CACAGGACAG CTCAAAGCTC AGCTCCTGTG TAATTACGTT 
CAGTCGCAGC AGGAATTGTT GTGGGAGGGC AGAAGCCGGG GAGGGATCCC CAACCAGGAA 
CTTCCATATC AGAGCCCCAA GTTTCTGGGA GGTTCTCAAG TTAGTCAGAG CCCCACCAAG 
GTCCCAGCAG CAGCAGCAGC AGCTACGTTT GGATCTGGCT TTGCATCACC TGCTTTGGCC 
AATCACAAAT CAGTCTCCTA TCCTGCCCCT TCACCGTGCC ATGAAACTTT CACCATGGGA 
GTAAACAGGC CTCCCCATAG GCCAGCAGCA CCACCCCGGC TTCTGCCCCC GCTACCACCT 
TGCTATGGGC CCCTCAAGGT GGGAGATGCC AACCCCAGCT GTGGGCATCC TGAAGTGGGC 
AGGTTAGGAG CAGGCCCTGC CTTGTACCCT CCCCCTGAAG GGCAGGTGTG TAACCCTCTG 
GACTCTCTTG ACCTGGACAA CACTCAGCTG GACTTTGTGG CTATCCTAGA TGAAGCTCAA 
GGGCTGAGCC CTCCTCCTTC CCACGAGCAA GGGGACAGCT CTAAAAACAC CCCACCTCCC 
TCCGGGCCCC CCAACATGGC AGTCGGTAAC ATGAGTGTCT TACTGGGGTC TCTGCCTGGA 
GAGACGCAGT TCCTCAACTC TAGTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR-873 suppresses H9C2 cardiomyocyte proliferation by targeting GLI1.
Gene626426-432(2017 Aug)
Zhang JS,Zhao Y,Lv Y,Liu PY,Ruan JX,Sun YL,Gong TX,Wan N,Qiu GR

Sonic Hedgehog Signaling Affected by Promoter Hypermethylation Induces Aberrant Gli2 Expression in Spina Bifida.
Molecular neurobiology53(8)5413-24(2016 Oct)
Lu XL,Wang L,Chang SY,Shangguan SF,Wang Z,Wu LH,Zou JZ,Xiao P,Li R,Bao YH,Qiu ZY,Zhang T

The Effect of SHH-Gli Signaling Pathway on the Synovial Fibroblast Proliferation in Rheumatoid Arthritis.
Inflammation39(2)503-12(2016 Apr)
Qin S,Sun D,Li H,Li X,Pan W,Yan C,Tang R,Liu X

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Gli1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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