Wnk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Wnk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wnk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa60691 XM_017592384.1
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa60692 XM_017592385.1
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa60693 XM_017592386.1
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa60694 XM_017592387.1
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa60695 XM_017592388.1
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa60696 XM_017592389.1
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa60697 XM_017592390.1
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa60698 XM_017592391.1
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa60699 XM_017592392.1
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ORa60700 XM_017592393.1
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ORa05838 NM_001002823.2
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
ORa08841 NM_001199095.1
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
ORa10663 NM_053794.2
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
ORa43975 XM_008763201.2
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
ORa43977 XM_008763203.2
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
ORa43979 XM_008763205.2
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
ORa43981 XM_008763207.2
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
ORa43985 XM_008763211.2
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
ORa43986 XM_008763212.2
Latest version!
Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa60691
    Clone ID Related Accession (Same CDS sequence) XM_017592384.1
    Accession Version XM_017592384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG AAGTTTAACT GTGCATCTGA GCAGGTGACA
    TTCAAACCTG GTGGCAGGAG GACGCGATTT CTGAGTACGC CCTGCTTGGC TCTTTGGAAG
    ATGGTGAAAA AAGTCTGTCC TTGCAACCAG CTCTGTATGT GCCCTCCAGC TGAACCAAAA
    TCAGGGACCA GCAGCACAAA CACCGTTGGA GGAACAGTGA GTAGCCAGGC TGCCCAGGCT
    CAGCCTCCTG CCATGACTTC CAGTAGGAAG GGCACATTCA CAGATGACCT GCACAAGTTG
    GTAGACAACT GGGCCCGAGA TGCCATGAAT CTTTCAGGCA GGAGAGGCAG CAAAGGACAC
    ATGAATTATG AGGGCCCTGG AATGGCAAGG AAGTTCTCTG CTCCTGGGCA GCTGTGCATT
    TCCATGACCT CAAACATGGG TGGCTCTACC CCCATCTCTG CAGCATCTGC TACCTCTCTA
    GGTCACTTCA CCAAGTCCAT GTGCCCCCCA CAGCAGTATG GTTTTCCAGC TGCCCCATTC
    GGCACTCAGT GGAGTGGAAC AGGTGGTCCA GCACCACAGC CACTTGGTCA GTTCCAACCT
    GTAGGAACCA CATCCTTGCA AAATTTCAAC ATCAGCAATT TGCAGAAGTC CATCAGCAAC
    CCCCCAGGTT CCAACCTACG GACCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447873.1
    CDS232..7521
    Translation

    Target ORF information:

    RefSeq Version XM_017592384.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG AAGTTTAACT GTGCATCTGA GCAGGTGACA
    TTCAAACCTG GTGGCAGGAG GACGCGATTT CTGAGTACGC CCTGCTTGGC TCTTTGGAAG
    ATGGTGAAAA AAGTCTGTCC TTGCAACCAG CTCTGTATGT GCCCTCCAGC TGAACCAAAA
    TCAGGGACCA GCAGCACAAA CACCGTTGGA GGAACAGTGA GTAGCCAGGC TGCCCAGGCT
    CAGCCTCCTG CCATGACTTC CAGTAGGAAG GGCACATTCA CAGATGACCT GCACAAGTTG
    GTAGACAACT GGGCCCGAGA TGCCATGAAT CTTTCAGGCA GGAGAGGCAG CAAAGGACAC
    ATGAATTATG AGGGCCCTGG AATGGCAAGG AAGTTCTCTG CTCCTGGGCA GCTGTGCATT
    TCCATGACCT CAAACATGGG TGGCTCTACC CCCATCTCTG CAGCATCTGC TACCTCTCTA
    GGTCACTTCA CCAAGTCCAT GTGCCCCCCA CAGCAGTATG GTTTTCCAGC TGCCCCATTC
    GGCACTCAGT GGAGTGGAAC AGGTGGTCCA GCACCACAGC CACTTGGTCA GTTCCAACCT
    GTAGGAACCA CATCCTTGCA AAATTTCAAC ATCAGCAATT TGCAGAAGTC CATCAGCAAC
    CCCCCAGGTT CCAACCTACG GACCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60692
    Clone ID Related Accession (Same CDS sequence) XM_017592385.1
    Accession Version XM_017592385.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGGGAATAC AGCCGACTGT ACCTCCTCAA CAGGCAGTAC AGTATTCACT TCCACAGGCA
    GCGTCTTCCA GTGAAGGCAC TGTCCAGCCA GTGAGTCAGC CTCAAGTGTC AGCTGGAACA
    CAGCTTCCAG TTTCCCAGAT AGTACCAACT GTCCAAGGTG AACCTCACAT CCCAGTTTCA
    ACACAACCCT CAGTTGTTCC AGTCCACTCT GGTGCTCATT TCCTTCCTAT GGGACAGCCA
    ATCCCTACTT CTTTACTCCC ACAGTATCCT GTCTCTCAAA TTTCCATATC GACTCCTCAT
    GTGTCCACGG CGCAAACAGG TTTCTCATCT GTTCCTATAA CAATGGCTGC TGGCATTAAT
    CAGCCTCTGC TCACCTTGGC TTCATCTGCT ACAGCATCTT CAATCCCAGG GGCATCAACT
    GTAGTTCCTA ATCAGCTTCC AACCCTTCTG CAGCCTGTGA ATCAGCTGCA GAGTCAGGTT
    CACCCACAGC TCCTACAGCC AACAACAGTT CAGTCCATGG GATTACCAGC TAACCTTGGA
    CAAGCTGCTG AGTGTCCACT TCCCCCTGGA GATGTTCTGT ACCAGGGCTT CCCATCTCGA
    CTGCCACCAC AATACCCAGG AGATTCAAAT ATTGCTCCCT CTTCCAACGT GGCTTCTGTT
    TGCATCCATT CTACAGTCCT AGCCCCTCCT CCTATGCCAA CAGAAGCATT GGCCACCCAA
    GGTTACTTTC CTACAGTAGT GCAGCCTTAT GTGGAATCAA CTCCTTTGGT TCCTATGGGC
    AGTGTAGGAG GCCAGGTTCA AGTGTCCCAA CCAGCAGTGA GTTTATCGCA ACAACCCCCC
    ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTAGTACAC AGGGAGTCTC TCAGGCTGCT
    CCTCCAGAAC AAACTCCAAT AACACAGTCA CAACCTACTC AACCTGTTCC ATTGGTTTCC
    TCTGTGGACA GTGCCCATTC AGATGTTGCT TCTGGTATGA GTGATGGCAA TGAGAATGCT
    CCTTCATCCA GCGGGAGGCA TGAAGGGAGG ACGACAAAAC GTCATTATCG AAAATCTGTA
    AGAAGTCGTT CTCGTCATGA AAAGACTTCT CGCCCGAAAC TGAGGATTTT AAATGTTTCA
    AATAAAGGAG ACCGAGTAGT AGAATGTCAA TTGGAGACTC ATAATCGGAA AATGGTTACA
    TTTAAATTTG ACCTAGATGG TGACAACCCT GAAGAGATAG CAACAATTAT GGTGAACAAT
    GACTTTATTC TAGCCATAGA GAGAGAGTCT TTTGTGGCTC AAGTGCGGGA AATTATTGAA
    AAGGCTGATG AAATGTTAAG CGAGGATGTC AGTGTGGAGC CTGAGGGTGA CCAGGGATTG
    GAGAGTCTAC AAGGAAAGGA TGACTATGGC TTTCCAGGTT CTCAGAAATT AGAAGGAGAG
    TTCAAGCAAC CAATTGCTGT ATCTTCCATG CCACAACAAA TAGGTGTTCC TACCAGTTCT
    TTAACACAAG TTGTTCATTC TGCTGGAAGA CGATTTATAG TGAGTCCAGT TCCAGAAAGT
    CGATTACGAG AATCAAAAAT TTTCACCAGT GAAATACCAG ATCCAGTTGC GGCCTCTACA
    TCTCAGGGCC CTGGAATGAA CTTATCTCAC TCCGCTTCAT CCCTTAGTCT CCAGCAGGCT
    TTTTCTGAAC TGAAACATGG TCAGATGACT GAAGGACCCA ATACAGCACC TCCAAATTTT
    AATCACCCAG GACCAACATT TTCTCCCTTT TTGACTAGCA TTGCTGGTGT CCAAACTGTA
    GCAGCATCCA CACCATCAGT ATCAGTCCCT ATAACAAGCA GCCCTCTTAA TGACATTTCC
    ACATCAGTGA TGCAGTCAGA GGGTGCACTG CCCACTGACA AAGGCATTGG GGGAGTTACC
    ACCAGCACAG GTGTGGTAGC TTCAGGTGGT CTCACCACAT TGTCTGTGTC TGAGACACCA
    ACACTTTCCA GTGCAGTTTC AAGTTCTACA GCGCCTGCAG TTGTCACAGT GTCTACAACA
    TCCCAACCAG TTCAGGCTTC CACATCTGGG AGCATTGCTT CTAGTACAGG CAGTTTTCCT
    TCTGGAACAT TTTCCACAAC TACAGGTACC ACAGTGAGTA GTGTAGCTGT CCCAAATGCT
    AAGCCTCCAA CTGTATTACT GCAACAGGTA GCAGGTAATA CTGCTGGGGT AGCCATAGTG
    ACATCAGTTT CTACTACCAC TCCATTCCCA GCCATGGCTT CACAGCCATC CCTTCCACTT
    GGTAGCAGCA CATCTGCTCC TACACTAGCT GAAACAGTGG TGGTTAGTGC ACACTCACTA
    GACAAGGCAT CTCACAGTAG TACAGCAGGA TTGGGTTTGT CCTTTTGCGC ACCGTCCTCT
    TCTTCCTCCT CTGGAACAGC AGTGTCTAGT TCTGTTTCTC AGCCTGGTAT TGTGCATCCT
    TTGGTCATCT CATCAGCTAT AGCTTCTACA CCTGTCCTCC CACAACCAGC AGTACCTACT
    TCTACACCTT TATTACCCCA AGTACCTAAT ATCCCACCCT TGGTACAACC TGTTGCTAAT
    GTGCCTGCTG TACAGCAAAC ACTCATTCAT AGTCAGCCTC AACCAGCTCT ACTTCCCAAC
    CAGCCTCACA CACACTGTCC CGAAATGGAT GCTGATACCC AGTCCAAAGC TCCTGGGATC
    GATGATATAA AGACTCTGGA GGAAAAGCTG AGATCGCTCT TTAGTGAGCA TAGCTCATCT
    GGAACTCAGC ATGCTTCTGT CTCCCTGGAG ACACCCTTAG TAGTAGAGAC TGTCACACCG
    GGCATCCCTA CCACTGCTGT AGCACCAAGT AAACTCATGA CTTCCACTAC AAGCACTTGC
    TTGCCACCAA CCAATTTACC CTTAGGAACA GCTGGCATGC CAGTTATGCC AGTGGGCACA
    CCTGGTCAAG TTTCTACCCC AGGCACACAT GCCTCAGCCC CAGCCAGCAC CGCAACAGGA
    GCAAAACCTG GAACAACTCC CCCAAAGCCA TCTTTAACTA AGACTGTGGT GCCGCCAGTG
    GGTACTGAGC TTTCGGCTGG TACTGTACCC TGTGAGCAGC TGCCACCTTT TCCCGGACCG
    TCTCTAATTC AGACCCAGCA ACCCCTAGAA GATCTTGATG CTCAGTTGAG AAGAACACTT
    AGTCCAGAGA CTATTCCAGT GACACCCGCA GTTGGTCCTT TGTCTACGAT GTCTTCAACG
    GCAGTTACAG AAGCAGGAAG TCAACCTCAG AAGGATGGCA CAGAAGTTCA TGTCACAGCA
    AGTAGCTCAG GAGCTGGTGT TGTTAAGATG GGGAGATTTC AGGTTTCAGT TACAATGGAT
    GATGCCCAGA AAGAGCGTAA AAATAGGTCA GAAGATACAA AGTCGGTTCA TTTTGAATCT
    AGTACATCAG AGTCATCAGT TCTTTCAAGT AGTAGTCCAG AGAGTACCCT GGTAAAGCCA
    GAGCCTAATG GAATTACTGT TTCTGGCATC TCCTTAGATG TGCCAGACAG TACCCATAGA
    ACACCGACCC CAGAAGCAAA GTCAGAAACA GGACAGCCTA CCAAGGTTGG ACGTTTTCAG
    GTGACAACTA CAGCCAACAA AGTGGGTCGT TTCTCTGTTT CAAGAACTGA GGACAAGGTC
    ACAGAGCTCA AAAAAGAGGG ACCAGTGACC TCTCCTTTCA GAGATTCAGA ACAAACTGTT
    ATTCCTGCTG CGATACCAAA GAAGGAGAAG CCTGAGCTGG CAGAACCTTC CCATCTGAAT
    GGGCCATCTT CAGACTTGGA GGCTGCCTTT CTAAGTAGGG GTGGAGAGGA TGGATCAGGT
    AGTCCTCACT CTCCCCCTCA CCTGTGTTCA AAGAGCCTTC CCATCCAGAC TCTAAGTCAA
    AGCCTTAGTA ATTCATTCAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC CGACATTGAA
    GATGAAGACT TAAGGTTAGA ACTGCGACGA CTACGGGAAA AACATCTTAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTT
    CCCCCTGCTG TCATTATTCC CCCAGCTGCT CCTCTGTCAG GGAGAAGAAG GAGGCCTACT
    AAAAGCAAAG GCAGCAAGTC CAGTCGCAGC AGCTCATTGG GCAACAAAAG CCCTCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGGAACT TCAGTCTTAA ACCCCCAACA GACCCTCCAT
    CCCCCAGGCA ATACCCCAGA GACTGGACAC AACCAGCTGT TACAGCCTCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTT ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG CTGTGCGAAG TTTAACTGTG CATCTGAGCA GGTGACATTC
    AAACCTGGTG GCAGGAGGAC GCGATTTCTG AGTACGCCCT GCTTGGCTCT TTGGAAGATG
    GTGAAAAAAG TCTGTCCTTG CAACCAGCTC TGTATGTGCC CTCCAGCTGA ACCAAAATCA
    GGGACCAGCA GCACAAACAC CGTTGGAGGA ACAGTGAGTA GCCAGGCTGC CCAGGCTCAG
    CCTCCTGCCA TGACTTCCAG TAGGAAGGGC ACATTCACAG ATGACCTGCA CAAGTTGGTA
    GACAACTGGG CCCGAGATGC CATGAATCTT TCAGGCAGGA GAGGCAGCAA AGGACACATG
    AATTATGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCTC CTGGGCAGCT GTGCATTTCC
    ATGACCTCAA ACATGGGTGG CTCTACCCCC ATCTCTGCAG CATCTGCTAC CTCTCTAGGT
    CACTTCACCA AGTCCATGTG CCCCCCACAG CAGTATGGTT TTCCAGCTGC CCCATTCGGC
    ACTCAGTGGA GTGGAACAGG TGGTCCAGCA CCACAGCCAC TTGGTCAGTT CCAACCTGTA
    GGAACCACAT CCTTGCAAAA TTTCAACATC AGCAATTTGC AGAAGTCCAT CAGCAACCCC
    CCAGGTTCCA ACCTACGGAC CACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447874.1
    CDS232..7518
    Translation

    Target ORF information:

    RefSeq Version XM_017592385.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592385.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGGGAATAC AGCCGACTGT ACCTCCTCAA CAGGCAGTAC AGTATTCACT TCCACAGGCA
    GCGTCTTCCA GTGAAGGCAC TGTCCAGCCA GTGAGTCAGC CTCAAGTGTC AGCTGGAACA
    CAGCTTCCAG TTTCCCAGAT AGTACCAACT GTCCAAGGTG AACCTCACAT CCCAGTTTCA
    ACACAACCCT CAGTTGTTCC AGTCCACTCT GGTGCTCATT TCCTTCCTAT GGGACAGCCA
    ATCCCTACTT CTTTACTCCC ACAGTATCCT GTCTCTCAAA TTTCCATATC GACTCCTCAT
    GTGTCCACGG CGCAAACAGG TTTCTCATCT GTTCCTATAA CAATGGCTGC TGGCATTAAT
    CAGCCTCTGC TCACCTTGGC TTCATCTGCT ACAGCATCTT CAATCCCAGG GGCATCAACT
    GTAGTTCCTA ATCAGCTTCC AACCCTTCTG CAGCCTGTGA ATCAGCTGCA GAGTCAGGTT
    CACCCACAGC TCCTACAGCC AACAACAGTT CAGTCCATGG GATTACCAGC TAACCTTGGA
    CAAGCTGCTG AGTGTCCACT TCCCCCTGGA GATGTTCTGT ACCAGGGCTT CCCATCTCGA
    CTGCCACCAC AATACCCAGG AGATTCAAAT ATTGCTCCCT CTTCCAACGT GGCTTCTGTT
    TGCATCCATT CTACAGTCCT AGCCCCTCCT CCTATGCCAA CAGAAGCATT GGCCACCCAA
    GGTTACTTTC CTACAGTAGT GCAGCCTTAT GTGGAATCAA CTCCTTTGGT TCCTATGGGC
    AGTGTAGGAG GCCAGGTTCA AGTGTCCCAA CCAGCAGTGA GTTTATCGCA ACAACCCCCC
    ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTAGTACAC AGGGAGTCTC TCAGGCTGCT
    CCTCCAGAAC AAACTCCAAT AACACAGTCA CAACCTACTC AACCTGTTCC ATTGGTTTCC
    TCTGTGGACA GTGCCCATTC AGATGTTGCT TCTGGTATGA GTGATGGCAA TGAGAATGCT
    CCTTCATCCA GCGGGAGGCA TGAAGGGAGG ACGACAAAAC GTCATTATCG AAAATCTGTA
    AGAAGTCGTT CTCGTCATGA AAAGACTTCT CGCCCGAAAC TGAGGATTTT AAATGTTTCA
    AATAAAGGAG ACCGAGTAGT AGAATGTCAA TTGGAGACTC ATAATCGGAA AATGGTTACA
    TTTAAATTTG ACCTAGATGG TGACAACCCT GAAGAGATAG CAACAATTAT GGTGAACAAT
    GACTTTATTC TAGCCATAGA GAGAGAGTCT TTTGTGGCTC AAGTGCGGGA AATTATTGAA
    AAGGCTGATG AAATGTTAAG CGAGGATGTC AGTGTGGAGC CTGAGGGTGA CCAGGGATTG
    GAGAGTCTAC AAGGAAAGGA TGACTATGGC TTTCCAGGTT CTCAGAAATT AGAAGGAGAG
    TTCAAGCAAC CAATTGCTGT ATCTTCCATG CCACAACAAA TAGGTGTTCC TACCAGTTCT
    TTAACACAAG TTGTTCATTC TGCTGGAAGA CGATTTATAG TGAGTCCAGT TCCAGAAAGT
    CGATTACGAG AATCAAAAAT TTTCACCAGT GAAATACCAG ATCCAGTTGC GGCCTCTACA
    TCTCAGGGCC CTGGAATGAA CTTATCTCAC TCCGCTTCAT CCCTTAGTCT CCAGCAGGCT
    TTTTCTGAAC TGAAACATGG TCAGATGACT GAAGGACCCA ATACAGCACC TCCAAATTTT
    AATCACCCAG GACCAACATT TTCTCCCTTT TTGACTAGCA TTGCTGGTGT CCAAACTGTA
    GCAGCATCCA CACCATCAGT ATCAGTCCCT ATAACAAGCA GCCCTCTTAA TGACATTTCC
    ACATCAGTGA TGCAGTCAGA GGGTGCACTG CCCACTGACA AAGGCATTGG GGGAGTTACC
    ACCAGCACAG GTGTGGTAGC TTCAGGTGGT CTCACCACAT TGTCTGTGTC TGAGACACCA
    ACACTTTCCA GTGCAGTTTC AAGTTCTACA GCGCCTGCAG TTGTCACAGT GTCTACAACA
    TCCCAACCAG TTCAGGCTTC CACATCTGGG AGCATTGCTT CTAGTACAGG CAGTTTTCCT
    TCTGGAACAT TTTCCACAAC TACAGGTACC ACAGTGAGTA GTGTAGCTGT CCCAAATGCT
    AAGCCTCCAA CTGTATTACT GCAACAGGTA GCAGGTAATA CTGCTGGGGT AGCCATAGTG
    ACATCAGTTT CTACTACCAC TCCATTCCCA GCCATGGCTT CACAGCCATC CCTTCCACTT
    GGTAGCAGCA CATCTGCTCC TACACTAGCT GAAACAGTGG TGGTTAGTGC ACACTCACTA
    GACAAGGCAT CTCACAGTAG TACAGCAGGA TTGGGTTTGT CCTTTTGCGC ACCGTCCTCT
    TCTTCCTCCT CTGGAACAGC AGTGTCTAGT TCTGTTTCTC AGCCTGGTAT TGTGCATCCT
    TTGGTCATCT CATCAGCTAT AGCTTCTACA CCTGTCCTCC CACAACCAGC AGTACCTACT
    TCTACACCTT TATTACCCCA AGTACCTAAT ATCCCACCCT TGGTACAACC TGTTGCTAAT
    GTGCCTGCTG TACAGCAAAC ACTCATTCAT AGTCAGCCTC AACCAGCTCT ACTTCCCAAC
    CAGCCTCACA CACACTGTCC CGAAATGGAT GCTGATACCC AGTCCAAAGC TCCTGGGATC
    GATGATATAA AGACTCTGGA GGAAAAGCTG AGATCGCTCT TTAGTGAGCA TAGCTCATCT
    GGAACTCAGC ATGCTTCTGT CTCCCTGGAG ACACCCTTAG TAGTAGAGAC TGTCACACCG
    GGCATCCCTA CCACTGCTGT AGCACCAAGT AAACTCATGA CTTCCACTAC AAGCACTTGC
    TTGCCACCAA CCAATTTACC CTTAGGAACA GCTGGCATGC CAGTTATGCC AGTGGGCACA
    CCTGGTCAAG TTTCTACCCC AGGCACACAT GCCTCAGCCC CAGCCAGCAC CGCAACAGGA
    GCAAAACCTG GAACAACTCC CCCAAAGCCA TCTTTAACTA AGACTGTGGT GCCGCCAGTG
    GGTACTGAGC TTTCGGCTGG TACTGTACCC TGTGAGCAGC TGCCACCTTT TCCCGGACCG
    TCTCTAATTC AGACCCAGCA ACCCCTAGAA GATCTTGATG CTCAGTTGAG AAGAACACTT
    AGTCCAGAGA CTATTCCAGT GACACCCGCA GTTGGTCCTT TGTCTACGAT GTCTTCAACG
    GCAGTTACAG AAGCAGGAAG TCAACCTCAG AAGGATGGCA CAGAAGTTCA TGTCACAGCA
    AGTAGCTCAG GAGCTGGTGT TGTTAAGATG GGGAGATTTC AGGTTTCAGT TACAATGGAT
    GATGCCCAGA AAGAGCGTAA AAATAGGTCA GAAGATACAA AGTCGGTTCA TTTTGAATCT
    AGTACATCAG AGTCATCAGT TCTTTCAAGT AGTAGTCCAG AGAGTACCCT GGTAAAGCCA
    GAGCCTAATG GAATTACTGT TTCTGGCATC TCCTTAGATG TGCCAGACAG TACCCATAGA
    ACACCGACCC CAGAAGCAAA GTCAGAAACA GGACAGCCTA CCAAGGTTGG ACGTTTTCAG
    GTGACAACTA CAGCCAACAA AGTGGGTCGT TTCTCTGTTT CAAGAACTGA GGACAAGGTC
    ACAGAGCTCA AAAAAGAGGG ACCAGTGACC TCTCCTTTCA GAGATTCAGA ACAAACTGTT
    ATTCCTGCTG CGATACCAAA GAAGGAGAAG CCTGAGCTGG CAGAACCTTC CCATCTGAAT
    GGGCCATCTT CAGACTTGGA GGCTGCCTTT CTAAGTAGGG GTGGAGAGGA TGGATCAGGT
    AGTCCTCACT CTCCCCCTCA CCTGTGTTCA AAGAGCCTTC CCATCCAGAC TCTAAGTCAA
    AGCCTTAGTA ATTCATTCAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC CGACATTGAA
    GATGAAGACT TAAGGTTAGA ACTGCGACGA CTACGGGAAA AACATCTTAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTT
    CCCCCTGCTG TCATTATTCC CCCAGCTGCT CCTCTGTCAG GGAGAAGAAG GAGGCCTACT
    AAAAGCAAAG GCAGCAAGTC CAGTCGCAGC AGCTCATTGG GCAACAAAAG CCCTCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGGAACT TCAGTCTTAA ACCCCCAACA GACCCTCCAT
    CCCCCAGGCA ATACCCCAGA GACTGGACAC AACCAGCTGT TACAGCCTCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTT ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG CTGTGCGAAG TTTAACTGTG CATCTGAGCA GGTGACATTC
    AAACCTGGTG GCAGGAGGAC GCGATTTCTG AGTACGCCCT GCTTGGCTCT TTGGAAGATG
    GTGAAAAAAG TCTGTCCTTG CAACCAGCTC TGTATGTGCC CTCCAGCTGA ACCAAAATCA
    GGGACCAGCA GCACAAACAC CGTTGGAGGA ACAGTGAGTA GCCAGGCTGC CCAGGCTCAG
    CCTCCTGCCA TGACTTCCAG TAGGAAGGGC ACATTCACAG ATGACCTGCA CAAGTTGGTA
    GACAACTGGG CCCGAGATGC CATGAATCTT TCAGGCAGGA GAGGCAGCAA AGGACACATG
    AATTATGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCTC CTGGGCAGCT GTGCATTTCC
    ATGACCTCAA ACATGGGTGG CTCTACCCCC ATCTCTGCAG CATCTGCTAC CTCTCTAGGT
    CACTTCACCA AGTCCATGTG CCCCCCACAG CAGTATGGTT TTCCAGCTGC CCCATTCGGC
    ACTCAGTGGA GTGGAACAGG TGGTCCAGCA CCACAGCCAC TTGGTCAGTT CCAACCTGTA
    GGAACCACAT CCTTGCAAAA TTTCAACATC AGCAATTTGC AGAAGTCCAT CAGCAACCCC
    CCAGGTTCCA ACCTACGGAC CACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60693
    Clone ID Related Accession (Same CDS sequence) XM_017592386.1
    Accession Version XM_017592386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG AAGTTTAACT GTGCATCTGA GCAGGTGACA
    TTCAAACCTG GTGGCAGGAG GACGCGATTT CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT
    TGCAACCAGC TCTGTATGTG CCCTCCAGCT GAACCAAAAT CAGGGACCAG CAGCACAAAC
    ACCGTTGGAG GAACAGTGAG TAGCCAGGCT GCCCAGGCTC AGCCTCCTGC CATGACTTCC
    AGTAGGAAGG GCACATTCAC AGATGACCTG CACAAGTTGG TAGACAACTG GGCCCGAGAT
    GCCATGAATC TTTCAGGCAG GAGAGGCAGC AAAGGACACA TGAATTATGA GGGCCCTGGA
    ATGGCAAGGA AGTTCTCTGC TCCTGGGCAG CTGTGCATTT CCATGACCTC AAACATGGGT
    GGCTCTACCC CCATCTCTGC AGCATCTGCT ACCTCTCTAG GTCACTTCAC CAAGTCCATG
    TGCCCCCCAC AGCAGTATGG TTTTCCAGCT GCCCCATTCG GCACTCAGTG GAGTGGAACA
    GGTGGTCCAG CACCACAGCC ACTTGGTCAG TTCCAACCTG TAGGAACCAC ATCCTTGCAA
    AATTTCAACA TCAGCAATTT GCAGAAGTCC ATCAGCAACC CCCCAGGTTC CAACCTACGG
    ACCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447875.1
    CDS232..7500
    Translation

    Target ORF information:

    RefSeq Version XM_017592386.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG AAGTTTAACT GTGCATCTGA GCAGGTGACA
    TTCAAACCTG GTGGCAGGAG GACGCGATTT CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT
    TGCAACCAGC TCTGTATGTG CCCTCCAGCT GAACCAAAAT CAGGGACCAG CAGCACAAAC
    ACCGTTGGAG GAACAGTGAG TAGCCAGGCT GCCCAGGCTC AGCCTCCTGC CATGACTTCC
    AGTAGGAAGG GCACATTCAC AGATGACCTG CACAAGTTGG TAGACAACTG GGCCCGAGAT
    GCCATGAATC TTTCAGGCAG GAGAGGCAGC AAAGGACACA TGAATTATGA GGGCCCTGGA
    ATGGCAAGGA AGTTCTCTGC TCCTGGGCAG CTGTGCATTT CCATGACCTC AAACATGGGT
    GGCTCTACCC CCATCTCTGC AGCATCTGCT ACCTCTCTAG GTCACTTCAC CAAGTCCATG
    TGCCCCCCAC AGCAGTATGG TTTTCCAGCT GCCCCATTCG GCACTCAGTG GAGTGGAACA
    GGTGGTCCAG CACCACAGCC ACTTGGTCAG TTCCAACCTG TAGGAACCAC ATCCTTGCAA
    AATTTCAACA TCAGCAATTT GCAGAAGTCC ATCAGCAACC CCCCAGGTTC CAACCTACGG
    ACCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60694
    Clone ID Related Accession (Same CDS sequence) XM_017592387.1
    Accession Version XM_017592387.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGCCTTT GTCTACGATG TCTTCAACGG CAGTTACAGA AGCAGGAAGT
    CAACCTCAGA AGGATGGCAC AGAAGTTCAT GTCACAGCAA GTAGCTCAGG AGCTGGTGTT
    GTTAAGATGG GGAGATTTCA GGTTTCAGTT ACAATGGATG ATGCCCAGAA AGAGCGTAAA
    AATAGGTCAG AAGATACAAA GTCGGTTCAT TTTGAATCTA GTACATCAGA GTCATCAGTT
    CTTTCAAGTA GTAGTCCAGA GAGTACCCTG GTAAAGCCAG AGCCTAATGG AATTACTGTT
    TCTGGCATCT CCTTAGATGT GCCAGACAGT ACCCATAGAA CACCGACCCC AGAAGCAAAG
    TCAGAAACAG GACAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCCAACAAA
    GTGGGTCGTT TCTCTGTTTC AAGAACTGAG GACAAGGTCA CAGAGCTCAA AAAAGAGGGA
    CCAGTGACCT CTCCTTTCAG AGATTCAGAA CAAACTGTTA TTCCTGCTGC GATACCAAAG
    AAGGAGAAGC CTGAGCTGGC AGAACCTTCC CATCTGAATG GGCCATCTTC AGACTTGGAG
    GCTGCCTTTC TAAGTAGGGG TGGAGAGGAT GGATCAGGTA GTCCTCACTC TCCCCCTCAC
    CTGTGTTCAA AGAGCCTTCC CATCCAGACT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC
    TCCTCTTACA TGAGTAGTGA CAATGAGTCC GACATTGAAG ATGAAGACTT AAGGTTAGAA
    CTGCGACGAC TACGGGAAAA ACATCTTAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACCAAACTG GGCAAGGTTC CCCCTGCTGT CATTATTCCC
    CCAGCTGCTC CTCTGTCAGG GAGAAGAAGG AGGCCTACTA AAAGCAAAGG CAGCAAGTCC
    AGTCGCAGCA GCTCATTGGG CAACAAAAGC CCTCAGCTTT CAGGTAACCT GTCTGGTCAG
    AGTGGAACTT CAGTCTTAAA CCCCCAACAG ACCCTCCATC CCCCAGGCAA TACCCCAGAG
    ACTGGACACA ACCAGCTGTT ACAGCCTCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAT
    TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTACCAAGCC TTTCTGCTCC AGGTCAAGGC
    TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACG
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC
    AACCAGCTCT GTATGTGCCC TCCAGCTGAA CCAAAATCAG GGACCAGCAG CACAAACACC
    GTTGGAGGAA CAGTGAGTAG CCAGGCTGCC CAGGCTCAGC CTCCTGCCAT GACTTCCAGT
    AGGAAGGGCA CATTCACAGA TGACCTGCAC AAGTTGGTAG ACAACTGGGC CCGAGATGCC
    ATGAATCTTT CAGGCAGGAG AGGCAGCAAA GGACACATGA ATTATGAGGG CCCTGGAATG
    GCAAGGAAGT TCTCTGCTCC TGGGCAGCTG TGCATTTCCA TGACCTCAAA CATGGGTGGC
    TCTACCCCCA TCTCTGCAGC ATCTGCTACC TCTCTAGGTC ACTTCACCAA GTCCATGTGC
    CCCCCACAGC AGTATGGTTT TCCAGCTGCC CCATTCGGCA CTCAGTGGAG TGGAACAGGT
    GGTCCAGCAC CACAGCCACT TGGTCAGTTC CAACCTGTAG GAACCACATC CTTGCAAAAT
    TTCAACATCA GCAATTTGCA GAAGTCCATC AGCAACCCCC CAGGTTCCAA CCTACGGACC
    ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447876.1
    CDS232..7437
    Translation

    Target ORF information:

    RefSeq Version XM_017592387.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592387.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGCCTTT GTCTACGATG TCTTCAACGG CAGTTACAGA AGCAGGAAGT
    CAACCTCAGA AGGATGGCAC AGAAGTTCAT GTCACAGCAA GTAGCTCAGG AGCTGGTGTT
    GTTAAGATGG GGAGATTTCA GGTTTCAGTT ACAATGGATG ATGCCCAGAA AGAGCGTAAA
    AATAGGTCAG AAGATACAAA GTCGGTTCAT TTTGAATCTA GTACATCAGA GTCATCAGTT
    CTTTCAAGTA GTAGTCCAGA GAGTACCCTG GTAAAGCCAG AGCCTAATGG AATTACTGTT
    TCTGGCATCT CCTTAGATGT GCCAGACAGT ACCCATAGAA CACCGACCCC AGAAGCAAAG
    TCAGAAACAG GACAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCCAACAAA
    GTGGGTCGTT TCTCTGTTTC AAGAACTGAG GACAAGGTCA CAGAGCTCAA AAAAGAGGGA
    CCAGTGACCT CTCCTTTCAG AGATTCAGAA CAAACTGTTA TTCCTGCTGC GATACCAAAG
    AAGGAGAAGC CTGAGCTGGC AGAACCTTCC CATCTGAATG GGCCATCTTC AGACTTGGAG
    GCTGCCTTTC TAAGTAGGGG TGGAGAGGAT GGATCAGGTA GTCCTCACTC TCCCCCTCAC
    CTGTGTTCAA AGAGCCTTCC CATCCAGACT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC
    TCCTCTTACA TGAGTAGTGA CAATGAGTCC GACATTGAAG ATGAAGACTT AAGGTTAGAA
    CTGCGACGAC TACGGGAAAA ACATCTTAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACCAAACTG GGCAAGGTTC CCCCTGCTGT CATTATTCCC
    CCAGCTGCTC CTCTGTCAGG GAGAAGAAGG AGGCCTACTA AAAGCAAAGG CAGCAAGTCC
    AGTCGCAGCA GCTCATTGGG CAACAAAAGC CCTCAGCTTT CAGGTAACCT GTCTGGTCAG
    AGTGGAACTT CAGTCTTAAA CCCCCAACAG ACCCTCCATC CCCCAGGCAA TACCCCAGAG
    ACTGGACACA ACCAGCTGTT ACAGCCTCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAT
    TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTACCAAGCC TTTCTGCTCC AGGTCAAGGC
    TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACG
    CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC
    AACCAGCTCT GTATGTGCCC TCCAGCTGAA CCAAAATCAG GGACCAGCAG CACAAACACC
    GTTGGAGGAA CAGTGAGTAG CCAGGCTGCC CAGGCTCAGC CTCCTGCCAT GACTTCCAGT
    AGGAAGGGCA CATTCACAGA TGACCTGCAC AAGTTGGTAG ACAACTGGGC CCGAGATGCC
    ATGAATCTTT CAGGCAGGAG AGGCAGCAAA GGACACATGA ATTATGAGGG CCCTGGAATG
    GCAAGGAAGT TCTCTGCTCC TGGGCAGCTG TGCATTTCCA TGACCTCAAA CATGGGTGGC
    TCTACCCCCA TCTCTGCAGC ATCTGCTACC TCTCTAGGTC ACTTCACCAA GTCCATGTGC
    CCCCCACAGC AGTATGGTTT TCCAGCTGCC CCATTCGGCA CTCAGTGGAG TGGAACAGGT
    GGTCCAGCAC CACAGCCACT TGGTCAGTTC CAACCTGTAG GAACCACATC CTTGCAAAAT
    TTCAACATCA GCAATTTGCA GAAGTCCATC AGCAACCCCC CAGGTTCCAA CCTACGGACC
    ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60695
    Clone ID Related Accession (Same CDS sequence) XM_017592388.1
    Accession Version XM_017592388.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGGAAGATG GTGAAAAAAG TCTGTCCTTG CAACCAGCTC
    TGTATGTGCC CTCCAGCTGA ACCAAAATCA GGGACCAGCA GCACAAACAC CGTTGGAGGA
    ACAGTGAGTA GCCAGGCTGC CCAGGCTCAG CCTCCTGCCA TGACTTCCAG TAGGAAGGGC
    ACATTCACAG ATGACCTGCA CAAGTTGGTA GACAACTGGG CCCGAGATGC CATGAATCTT
    TCAGGCAGGA GAGGCAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGGAAG
    TTCTCTGCTC CTGGGCAGCT GTGCATTTCC ATGACCTCAA ACATGGGTGG CTCTACCCCC
    ATCTCTGCAG CATCTGCTAC CTCTCTAGGT CACTTCACCA AGTCCATGTG CCCCCCACAG
    CAGTATGGTT TTCCAGCTGC CCCATTCGGC ACTCAGTGGA GTGGAACAGG TGGTCCAGCA
    CCACAGCCAC TTGGTCAGTT CCAACCTGTA GGAACCACAT CCTTGCAAAA TTTCAACATC
    AGCAATTTGC AGAAGTCCAT CAGCAACCCC CCAGGTTCCA ACCTACGGAC CACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447877.1
    CDS232..7428
    Translation

    Target ORF information:

    RefSeq Version XM_017592388.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592388.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGGAAGATG GTGAAAAAAG TCTGTCCTTG CAACCAGCTC
    TGTATGTGCC CTCCAGCTGA ACCAAAATCA GGGACCAGCA GCACAAACAC CGTTGGAGGA
    ACAGTGAGTA GCCAGGCTGC CCAGGCTCAG CCTCCTGCCA TGACTTCCAG TAGGAAGGGC
    ACATTCACAG ATGACCTGCA CAAGTTGGTA GACAACTGGG CCCGAGATGC CATGAATCTT
    TCAGGCAGGA GAGGCAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGGAAG
    TTCTCTGCTC CTGGGCAGCT GTGCATTTCC ATGACCTCAA ACATGGGTGG CTCTACCCCC
    ATCTCTGCAG CATCTGCTAC CTCTCTAGGT CACTTCACCA AGTCCATGTG CCCCCCACAG
    CAGTATGGTT TTCCAGCTGC CCCATTCGGC ACTCAGTGGA GTGGAACAGG TGGTCCAGCA
    CCACAGCCAC TTGGTCAGTT CCAACCTGTA GGAACCACAT CCTTGCAAAA TTTCAACATC
    AGCAATTTGC AGAAGTCCAT CAGCAACCCC CCAGGTTCCA ACCTACGGAC CACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60696
    Clone ID Related Accession (Same CDS sequence) XM_017592389.1
    Accession Version XM_017592389.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG AAGTTTAACT GTGCATCTGA GCAGGTGACA
    TTCAAACCTG GTGGCAGGAG GACGCGATTT CTGAGTACGC CCTGCTTGGC TCTTTGGAAG
    ATGGTGAAAA AAGTCTGTCC TTGCAACCAG CTCTGTATGT GCCCTCCAGC TGAACCAAAA
    TCAGGGACCA GCAGCACAAA CACCGTTGGA GGAACAGTGA GTAGCCAGGC TGCCCAGGCT
    CAGCCTCCTG CCATGACTTC CAGTAGGAAG GGCACATTCA CAGATGACCT GCACAAGTTG
    GTAGACAACT GGGCCCGAGA TGCCATGAAT CTTTCAGGCA GGAGAGGCAG CAAAGGACAC
    ATGAATTATG AGGTGAGTCT CTCCTTTTCC AACCTGCCAG ACTACCAACA CACAGATGTG
    TCAGTTTATA GCTGGATTTA TTGTTTCTGG GAACCTTTAG TAGCAGTTGC AAGGATGAAA
    GTGGTACTTT CCCCTCAGGA AAAAAAAGGC AAAGCAGCTG TTAGGAGGCA GGGAAATTAT
    GGCAAACGGC TGTCAGTCAA CAGTTCTGTA AGCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447878.1
    CDS232..7410
    Translation

    Target ORF information:

    RefSeq Version XM_017592389.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592389.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG AAGTTTAACT GTGCATCTGA GCAGGTGACA
    TTCAAACCTG GTGGCAGGAG GACGCGATTT CTGAGTACGC CCTGCTTGGC TCTTTGGAAG
    ATGGTGAAAA AAGTCTGTCC TTGCAACCAG CTCTGTATGT GCCCTCCAGC TGAACCAAAA
    TCAGGGACCA GCAGCACAAA CACCGTTGGA GGAACAGTGA GTAGCCAGGC TGCCCAGGCT
    CAGCCTCCTG CCATGACTTC CAGTAGGAAG GGCACATTCA CAGATGACCT GCACAAGTTG
    GTAGACAACT GGGCCCGAGA TGCCATGAAT CTTTCAGGCA GGAGAGGCAG CAAAGGACAC
    ATGAATTATG AGGTGAGTCT CTCCTTTTCC AACCTGCCAG ACTACCAACA CACAGATGTG
    TCAGTTTATA GCTGGATTTA TTGTTTCTGG GAACCTTTAG TAGCAGTTGC AAGGATGAAA
    GTGGTACTTT CCCCTCAGGA AAAAAAAGGC AAAGCAGCTG TTAGGAGGCA GGGAAATTAT
    GGCAAACGGC TGTCAGTCAA CAGTTCTGTA AGCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60697
    Clone ID Related Accession (Same CDS sequence) XM_017592390.1
    Accession Version XM_017592390.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7122bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GCCTTTGTCT
    ACGATGTCTT CAACGGCAGT TACAGAAGCA GGAAGTCAAC CTCAGAAGGA TGGCACAGAA
    GTTCATGTCA CAGCAAGTAG CTCAGGAGCT GGTGTTGTTA AGATGGGGAG ATTTCAGGTT
    TCAGTTACAA TGGATGATGC CCAGAAAGAG CGTAAAAATA GGTCAGAAGA TACAAAGTCG
    GTTCATTTTG AATCTAGTAC ATCAGAGTCA TCAGTTCTTT CAAGTAGTAG TCCAGAGAGT
    ACCCTGGTAA AGCCAGAGCC TAATGGAATT ACTGTTTCTG GCATCTCCTT AGATGTGCCA
    GACAGTACCC ATAGAACACC GACCCCAGAA GCAAAGTCAG AAACAGGACA GCCTACCAAG
    GTTGGACGTT TTCAGGTGAC AACTACAGCC AACAAAGTGG GTCGTTTCTC TGTTTCAAGA
    ACTGAGGACA AGGTCACAGA GCTCAAAAAA GAGGGACCAG TGACCTCTCC TTTCAGAGAT
    TCAGAACAAA CTGTTATTCC TGCTGCGATA CCAAAGAAGG AGAAGCCTGA GCTGGCAGAA
    CCTTCCCATC TGAATGGGCC ATCTTCAGAC TTGGAGGCTG CCTTTCTAAG TAGGGGTGGA
    GAGGATGGAT CAGGTAGTCC TCACTCTCCC CCTCACCTGT GTTCAAAGAG CCTTCCCATC
    CAGACTCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT
    GAGTCCGACA TTGAAGATGA AGACTTAAGG TTAGAACTGC GACGACTACG GGAAAAACAT
    CTTAAAGAGA TTCAGGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC
    AAACTGGGCA AGGTTCCCCC TGCTGTCATT ATTCCCCCAG CTGCTCCTCT GTCAGGGAGA
    AGAAGGAGGC CTACTAAAAG CAAAGGCAGC AAGTCCAGTC GCAGCAGCTC ATTGGGCAAC
    AAAAGCCCTC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG GAACTTCAGT CTTAAACCCC
    CAACAGACCC TCCATCCCCC AGGCAATACC CCAGAGACTG GACACAACCA GCTGTTACAG
    CCTCTTAAGC CATCTCCCTC CAGTGACAAC CTCTATTCAG CCTTTACCAG TGATGGTGCC
    ATTTCAGTAC CAAGCCTTTC TGCTCCAGGT CAAGGCTGTG CGAAGTTTAA CTGTGCATCT
    GAGCAGGTGA CATTCAAACC TGGTGGCAGG AGGACGCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAT GTGCCCTCCA
    GCTGAACCAA AATCAGGGAC CAGCAGCACA AACACCGTTG GAGGAACAGT GAGTAGCCAG
    GCTGCCCAGG CTCAGCCTCC TGCCATGACT TCCAGTAGGA AGGGCACATT CACAGATGAC
    CTGCACAAGT TGGTAGACAA CTGGGCCCGA GATGCCATGA ATCTTTCAGG CAGGAGAGGC
    AGCAAAGGAC ACATGAATTA TGAGGGCCCT GGAATGGCAA GGAAGTTCTC TGCTCCTGGG
    CAGCTGTGCA TTTCCATGAC CTCAAACATG GGTGGCTCTA CCCCCATCTC TGCAGCATCT
    GCTACCTCTC TAGGTCACTT CACCAAGTCC ATGTGCCCCC CACAGCAGTA TGGTTTTCCA
    GCTGCCCCAT TCGGCACTCA GTGGAGTGGA ACAGGTGGTC CAGCACCACA GCCACTTGGT
    CAGTTCCAAC CTGTAGGAAC CACATCCTTG CAAAATTTCA ACATCAGCAA TTTGCAGAAG
    TCCATCAGCA ACCCCCCAGG TTCCAACCTA CGGACCACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447879.1
    CDS232..7353
    Translation

    Target ORF information:

    RefSeq Version XM_017592390.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592390.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GCCTTTGTCT
    ACGATGTCTT CAACGGCAGT TACAGAAGCA GGAAGTCAAC CTCAGAAGGA TGGCACAGAA
    GTTCATGTCA CAGCAAGTAG CTCAGGAGCT GGTGTTGTTA AGATGGGGAG ATTTCAGGTT
    TCAGTTACAA TGGATGATGC CCAGAAAGAG CGTAAAAATA GGTCAGAAGA TACAAAGTCG
    GTTCATTTTG AATCTAGTAC ATCAGAGTCA TCAGTTCTTT CAAGTAGTAG TCCAGAGAGT
    ACCCTGGTAA AGCCAGAGCC TAATGGAATT ACTGTTTCTG GCATCTCCTT AGATGTGCCA
    GACAGTACCC ATAGAACACC GACCCCAGAA GCAAAGTCAG AAACAGGACA GCCTACCAAG
    GTTGGACGTT TTCAGGTGAC AACTACAGCC AACAAAGTGG GTCGTTTCTC TGTTTCAAGA
    ACTGAGGACA AGGTCACAGA GCTCAAAAAA GAGGGACCAG TGACCTCTCC TTTCAGAGAT
    TCAGAACAAA CTGTTATTCC TGCTGCGATA CCAAAGAAGG AGAAGCCTGA GCTGGCAGAA
    CCTTCCCATC TGAATGGGCC ATCTTCAGAC TTGGAGGCTG CCTTTCTAAG TAGGGGTGGA
    GAGGATGGAT CAGGTAGTCC TCACTCTCCC CCTCACCTGT GTTCAAAGAG CCTTCCCATC
    CAGACTCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT
    GAGTCCGACA TTGAAGATGA AGACTTAAGG TTAGAACTGC GACGACTACG GGAAAAACAT
    CTTAAAGAGA TTCAGGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC
    AAACTGGGCA AGGTTCCCCC TGCTGTCATT ATTCCCCCAG CTGCTCCTCT GTCAGGGAGA
    AGAAGGAGGC CTACTAAAAG CAAAGGCAGC AAGTCCAGTC GCAGCAGCTC ATTGGGCAAC
    AAAAGCCCTC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG GAACTTCAGT CTTAAACCCC
    CAACAGACCC TCCATCCCCC AGGCAATACC CCAGAGACTG GACACAACCA GCTGTTACAG
    CCTCTTAAGC CATCTCCCTC CAGTGACAAC CTCTATTCAG CCTTTACCAG TGATGGTGCC
    ATTTCAGTAC CAAGCCTTTC TGCTCCAGGT CAAGGCTGTG CGAAGTTTAA CTGTGCATCT
    GAGCAGGTGA CATTCAAACC TGGTGGCAGG AGGACGCGAT TTCTGAGTAC GCCCTGCTTG
    GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAT GTGCCCTCCA
    GCTGAACCAA AATCAGGGAC CAGCAGCACA AACACCGTTG GAGGAACAGT GAGTAGCCAG
    GCTGCCCAGG CTCAGCCTCC TGCCATGACT TCCAGTAGGA AGGGCACATT CACAGATGAC
    CTGCACAAGT TGGTAGACAA CTGGGCCCGA GATGCCATGA ATCTTTCAGG CAGGAGAGGC
    AGCAAAGGAC ACATGAATTA TGAGGGCCCT GGAATGGCAA GGAAGTTCTC TGCTCCTGGG
    CAGCTGTGCA TTTCCATGAC CTCAAACATG GGTGGCTCTA CCCCCATCTC TGCAGCATCT
    GCTACCTCTC TAGGTCACTT CACCAAGTCC ATGTGCCCCC CACAGCAGTA TGGTTTTCCA
    GCTGCCCCAT TCGGCACTCA GTGGAGTGGA ACAGGTGGTC CAGCACCACA GCCACTTGGT
    CAGTTCCAAC CTGTAGGAAC CACATCCTTG CAAAATTTCA ACATCAGCAA TTTGCAGAAG
    TCCATCAGCA ACCCCCCAGG TTCCAACCTA CGGACCACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60698
    Clone ID Related Accession (Same CDS sequence) XM_017592391.1
    Accession Version XM_017592391.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGAG TAGTACACAG
    GGAGTCTCTC AGGCTGCTCC TCCAGAACAA ACTCCAATAA CACAGTCACA ACCTACTCAA
    CCTGTTCCAT TGGTTTCCTC TGTGGACAGT GCCCATTCAG ATGTTGCTTC TGGTATGAGT
    GATGGCAATG AGAATGCTCC TTCATCCAGC GGGAGGCATG AAGGGAGGAC GACAAAACGT
    CATTATCGAA AATCTGTAAG AAGTCGTTCT CGTCATGAAA AGACTTCTCG CCCGAAACTG
    AGGATTTTAA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT GGAGACTCAT
    AATCGGAAAA TGGTTACATT TAAATTTGAC CTAGATGGTG ACAACCCTGA AGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCCATAGAGA GAGAGTCTTT TGTGGCTCAA
    GTGCGGGAAA TTATTGAAAA GGCTGATGAA ATGTTAAGCG AGGATGTCAG TGTGGAGCCT
    GAGGGTGACC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TCCAGGTTCT
    CAGAAATTAG AAGGAGAGTT CAAGCAACCA ATTGCTGTAT CTTCCATGCC ACAACAAATA
    GGTGTTCCTA CCAGTTCTTT AACACAAGTT GTTCATTCTG CTGGAAGACG ATTTATAGTG
    AGTCCAGTTC CAGAAAGTCG ATTACGAGAA TCAAAAATTT TCACCAGTGA AATACCAGAT
    CCAGTTGCGG CCTCTACATC TCAGGGCCCT GGAATGAACT TATCTCACTC CGCTTCATCC
    CTTAGTCTCC AGCAGGCTTT TTCTGAACTG AAACATGGTC AGATGACTGA AGGACCCAAT
    ACAGCACCTC CAAATTTTAA TCACCCAGGA CCAACATTTT CTCCCTTTTT GACTAGCATT
    GCTGGTGTCC AAACTGTAGC AGCATCCACA CCATCAGTAT CAGTCCCTAT AACAAGCAGC
    CCTCTTAATG ACATTTCCAC ATCAGTGATG CAGTCAGAGG GTGCACTGCC CACTGACAAA
    GGCATTGGGG GAGTTACCAC CAGCACAGGT GTGGTAGCTT CAGGTGGTCT CACCACATTG
    TCTGTGTCTG AGACACCAAC ACTTTCCAGT GCAGTTTCAA GTTCTACAGC GCCTGCAGTT
    GTCACAGTGT CTACAACATC CCAACCAGTT CAGGCTTCCA CATCTGGGAG CATTGCTTCT
    AGTACAGGCA GTTTTCCTTC TGGAACATTT TCCACAACTA CAGGTACCAC AGTGAGTAGT
    GTAGCTGTCC CAAATGCTAA GCCTCCAACT GTATTACTGC AACAGGTAGC AGGTAATACT
    GCTGGGGTAG CCATAGTGAC ATCAGTTTCT ACTACCACTC CATTCCCAGC CATGGCTTCA
    CAGCCATCCC TTCCACTTGG TAGCAGCACA TCTGCTCCTA CACTAGCTGA AACAGTGGTG
    GTTAGTGCAC ACTCACTAGA CAAGGCATCT CACAGTAGTA CAGCAGGATT GGGTTTGTCC
    TTTTGCGCAC CGTCCTCTTC TTCCTCCTCT GGAACAGCAG TGTCTAGTTC TGTTTCTCAG
    CCTGGTATTG TGCATCCTTT GGTCATCTCA TCAGCTATAG CTTCTACACC TGTCCTCCCA
    CAACCAGCAG TACCTACTTC TACACCTTTA TTACCCCAAG TACCTAATAT CCCACCCTTG
    GTACAACCTG TTGCTAATGT GCCTGCTGTA CAGCAAACAC TCATTCATAG TCAGCCTCAA
    CCAGCTCTAC TTCCCAACCA GCCTCACACA CACTGTCCCG AAATGGATGC TGATACCCAG
    TCCAAAGCTC CTGGGATCGA TGATATAAAG ACTCTGGAGG AAAAGCTGAG ATCGCTCTTT
    AGTGAGCATA GCTCATCTGG AACTCAGCAT GCTTCTGTCT CCCTGGAGAC ACCCTTAGTA
    GTAGAGACTG TCACACCGGG CATCCCTACC ACTGCTGTAG CACCAAGTAA ACTCATGACT
    TCCACTACAA GCACTTGCTT GCCACCAACC AATTTACCCT TAGGAACAGC TGGCATGCCA
    GTTATGCCAG TGGGCACACC TGGTCAAGTT TCTACCCCAG GCACACATGC CTCAGCCCCA
    GCCAGCACCG CAACAGGAGC AAAACCTGGA ACAACTCCCC CAAAGCCATC TTTAACTAAG
    ACTGTGGTGC CGCCAGTGGG TACTGAGCTT TCGGCTGGTA CTGTACCCTG TGAGCAGCTG
    CCACCTTTTC CCGGACCGTC TCTAATTCAG ACCCAGCAAC CCCTAGAAGA TCTTGATGCT
    CAGTTGAGAA GAACACTTAG TCCAGAGACT ATTCCAGTGA CACCCGCAGT TGGTCCTTTG
    TCTACGATGT CTTCAACGGC AGTTACAGAA GCAGGAAGTC AACCTCAGAA GGATGGCACA
    GAAGTTCATG TCACAGCAAG TAGCTCAGGA GCTGGTGTTG TTAAGATGGG GAGATTTCAG
    GTTTCAGTTA CAATGGATGA TGCCCAGAAA GAGCGTAAAA ATAGGTCAGA AGATACAAAG
    TCGGTTCATT TTGAATCTAG TACATCAGAG TCATCAGTTC TTTCAAGTAG TAGTCCAGAG
    AGTACCCTGG TAAAGCCAGA GCCTAATGGA ATTACTGTTT CTGGCATCTC CTTAGATGTG
    CCAGACAGTA CCCATAGAAC ACCGACCCCA GAAGCAAAGT CAGAAACAGG ACAGCCTACC
    AAGGTTGGAC GTTTTCAGGT GACAACTACA GCCAACAAAG TGGGTCGTTT CTCTGTTTCA
    AGAACTGAGG ACAAGGTCAC AGAGCTCAAA AAAGAGGGAC CAGTGACCTC TCCTTTCAGA
    GATTCAGAAC AAACTGTTAT TCCTGCTGCG ATACCAAAGA AGGAGAAGCC TGAGCTGGCA
    GAACCTTCCC ATCTGAATGG GCCATCTTCA GACTTGGAGG CTGCCTTTCT AAGTAGGGGT
    GGAGAGGATG GATCAGGTAG TCCTCACTCT CCCCCTCACC TGTGTTCAAA GAGCCTTCCC
    ATCCAGACTC TAAGTCAAAG CCTTAGTAAT TCATTCAACT CCTCTTACAT GAGTAGTGAC
    AATGAGTCCG ACATTGAAGA TGAAGACTTA AGGTTAGAAC TGCGACGACT ACGGGAAAAA
    CATCTTAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT
    ACCAAACTGG GCAAGGTTCC CCCTGCTGTC ATTATTCCCC CAGCTGCTCC TCTGTCAGGG
    AGAAGAAGGA GGCCTACTAA AAGCAAAGGC AGCAAGTCCA GTCGCAGCAG CTCATTGGGC
    AACAAAAGCC CTCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGGAACTTC AGTCTTAAAC
    CCCCAACAGA CCCTCCATCC CCCAGGCAAT ACCCCAGAGA CTGGACACAA CCAGCTGTTA
    CAGCCTCTTA AGCCATCTCC CTCCAGTGAC AACCTCTATT CAGCCTTTAC CAGTGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCTCCA GGTCAAGGCT GTGCGAAGTT TAACTGTGCA
    TCTGAGCAGG TGACATTCAA ACCTGGTGGC AGGAGGACGC GATTTCTGAG TACGCCCTGC
    TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TATGTGCCCT
    CCAGCTGAAC CAAAATCAGG GACCAGCAGC ACAAACACCG TTGGAGGAAC AGTGAGTAGC
    CAGGCTGCCC AGGCTCAGCC TCCTGCCATG ACTTCCAGTA GGAAGGGCAC ATTCACAGAT
    GACCTGCACA AGTTGGTAGA CAACTGGGCC CGAGATGCCA TGAATCTTTC AGGCAGGAGA
    GGCAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCTCCT
    GGGCAGCTGT GCATTTCCAT GACCTCAAAC ATGGGTGGCT CTACCCCCAT CTCTGCAGCA
    TCTGCTACCT CTCTAGGTCA CTTCACCAAG TCCATGTGCC CCCCACAGCA GTATGGTTTT
    CCAGCTGCCC CATTCGGCAC TCAGTGGAGT GGAACAGGTG GTCCAGCACC ACAGCCACTT
    GGTCAGTTCC AACCTGTAGG AACCACATCC TTGCAAAATT TCAACATCAG CAATTTGCAG
    AAGTCCATCA GCAACCCCCC AGGTTCCAAC CTACGGACCA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447880.1
    CDS232..7236
    Translation

    Target ORF information:

    RefSeq Version XM_017592391.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592391.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGAG TAGTACACAG
    GGAGTCTCTC AGGCTGCTCC TCCAGAACAA ACTCCAATAA CACAGTCACA ACCTACTCAA
    CCTGTTCCAT TGGTTTCCTC TGTGGACAGT GCCCATTCAG ATGTTGCTTC TGGTATGAGT
    GATGGCAATG AGAATGCTCC TTCATCCAGC GGGAGGCATG AAGGGAGGAC GACAAAACGT
    CATTATCGAA AATCTGTAAG AAGTCGTTCT CGTCATGAAA AGACTTCTCG CCCGAAACTG
    AGGATTTTAA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT GGAGACTCAT
    AATCGGAAAA TGGTTACATT TAAATTTGAC CTAGATGGTG ACAACCCTGA AGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCCATAGAGA GAGAGTCTTT TGTGGCTCAA
    GTGCGGGAAA TTATTGAAAA GGCTGATGAA ATGTTAAGCG AGGATGTCAG TGTGGAGCCT
    GAGGGTGACC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TCCAGGTTCT
    CAGAAATTAG AAGGAGAGTT CAAGCAACCA ATTGCTGTAT CTTCCATGCC ACAACAAATA
    GGTGTTCCTA CCAGTTCTTT AACACAAGTT GTTCATTCTG CTGGAAGACG ATTTATAGTG
    AGTCCAGTTC CAGAAAGTCG ATTACGAGAA TCAAAAATTT TCACCAGTGA AATACCAGAT
    CCAGTTGCGG CCTCTACATC TCAGGGCCCT GGAATGAACT TATCTCACTC CGCTTCATCC
    CTTAGTCTCC AGCAGGCTTT TTCTGAACTG AAACATGGTC AGATGACTGA AGGACCCAAT
    ACAGCACCTC CAAATTTTAA TCACCCAGGA CCAACATTTT CTCCCTTTTT GACTAGCATT
    GCTGGTGTCC AAACTGTAGC AGCATCCACA CCATCAGTAT CAGTCCCTAT AACAAGCAGC
    CCTCTTAATG ACATTTCCAC ATCAGTGATG CAGTCAGAGG GTGCACTGCC CACTGACAAA
    GGCATTGGGG GAGTTACCAC CAGCACAGGT GTGGTAGCTT CAGGTGGTCT CACCACATTG
    TCTGTGTCTG AGACACCAAC ACTTTCCAGT GCAGTTTCAA GTTCTACAGC GCCTGCAGTT
    GTCACAGTGT CTACAACATC CCAACCAGTT CAGGCTTCCA CATCTGGGAG CATTGCTTCT
    AGTACAGGCA GTTTTCCTTC TGGAACATTT TCCACAACTA CAGGTACCAC AGTGAGTAGT
    GTAGCTGTCC CAAATGCTAA GCCTCCAACT GTATTACTGC AACAGGTAGC AGGTAATACT
    GCTGGGGTAG CCATAGTGAC ATCAGTTTCT ACTACCACTC CATTCCCAGC CATGGCTTCA
    CAGCCATCCC TTCCACTTGG TAGCAGCACA TCTGCTCCTA CACTAGCTGA AACAGTGGTG
    GTTAGTGCAC ACTCACTAGA CAAGGCATCT CACAGTAGTA CAGCAGGATT GGGTTTGTCC
    TTTTGCGCAC CGTCCTCTTC TTCCTCCTCT GGAACAGCAG TGTCTAGTTC TGTTTCTCAG
    CCTGGTATTG TGCATCCTTT GGTCATCTCA TCAGCTATAG CTTCTACACC TGTCCTCCCA
    CAACCAGCAG TACCTACTTC TACACCTTTA TTACCCCAAG TACCTAATAT CCCACCCTTG
    GTACAACCTG TTGCTAATGT GCCTGCTGTA CAGCAAACAC TCATTCATAG TCAGCCTCAA
    CCAGCTCTAC TTCCCAACCA GCCTCACACA CACTGTCCCG AAATGGATGC TGATACCCAG
    TCCAAAGCTC CTGGGATCGA TGATATAAAG ACTCTGGAGG AAAAGCTGAG ATCGCTCTTT
    AGTGAGCATA GCTCATCTGG AACTCAGCAT GCTTCTGTCT CCCTGGAGAC ACCCTTAGTA
    GTAGAGACTG TCACACCGGG CATCCCTACC ACTGCTGTAG CACCAAGTAA ACTCATGACT
    TCCACTACAA GCACTTGCTT GCCACCAACC AATTTACCCT TAGGAACAGC TGGCATGCCA
    GTTATGCCAG TGGGCACACC TGGTCAAGTT TCTACCCCAG GCACACATGC CTCAGCCCCA
    GCCAGCACCG CAACAGGAGC AAAACCTGGA ACAACTCCCC CAAAGCCATC TTTAACTAAG
    ACTGTGGTGC CGCCAGTGGG TACTGAGCTT TCGGCTGGTA CTGTACCCTG TGAGCAGCTG
    CCACCTTTTC CCGGACCGTC TCTAATTCAG ACCCAGCAAC CCCTAGAAGA TCTTGATGCT
    CAGTTGAGAA GAACACTTAG TCCAGAGACT ATTCCAGTGA CACCCGCAGT TGGTCCTTTG
    TCTACGATGT CTTCAACGGC AGTTACAGAA GCAGGAAGTC AACCTCAGAA GGATGGCACA
    GAAGTTCATG TCACAGCAAG TAGCTCAGGA GCTGGTGTTG TTAAGATGGG GAGATTTCAG
    GTTTCAGTTA CAATGGATGA TGCCCAGAAA GAGCGTAAAA ATAGGTCAGA AGATACAAAG
    TCGGTTCATT TTGAATCTAG TACATCAGAG TCATCAGTTC TTTCAAGTAG TAGTCCAGAG
    AGTACCCTGG TAAAGCCAGA GCCTAATGGA ATTACTGTTT CTGGCATCTC CTTAGATGTG
    CCAGACAGTA CCCATAGAAC ACCGACCCCA GAAGCAAAGT CAGAAACAGG ACAGCCTACC
    AAGGTTGGAC GTTTTCAGGT GACAACTACA GCCAACAAAG TGGGTCGTTT CTCTGTTTCA
    AGAACTGAGG ACAAGGTCAC AGAGCTCAAA AAAGAGGGAC CAGTGACCTC TCCTTTCAGA
    GATTCAGAAC AAACTGTTAT TCCTGCTGCG ATACCAAAGA AGGAGAAGCC TGAGCTGGCA
    GAACCTTCCC ATCTGAATGG GCCATCTTCA GACTTGGAGG CTGCCTTTCT AAGTAGGGGT
    GGAGAGGATG GATCAGGTAG TCCTCACTCT CCCCCTCACC TGTGTTCAAA GAGCCTTCCC
    ATCCAGACTC TAAGTCAAAG CCTTAGTAAT TCATTCAACT CCTCTTACAT GAGTAGTGAC
    AATGAGTCCG ACATTGAAGA TGAAGACTTA AGGTTAGAAC TGCGACGACT ACGGGAAAAA
    CATCTTAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT
    ACCAAACTGG GCAAGGTTCC CCCTGCTGTC ATTATTCCCC CAGCTGCTCC TCTGTCAGGG
    AGAAGAAGGA GGCCTACTAA AAGCAAAGGC AGCAAGTCCA GTCGCAGCAG CTCATTGGGC
    AACAAAAGCC CTCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGGAACTTC AGTCTTAAAC
    CCCCAACAGA CCCTCCATCC CCCAGGCAAT ACCCCAGAGA CTGGACACAA CCAGCTGTTA
    CAGCCTCTTA AGCCATCTCC CTCCAGTGAC AACCTCTATT CAGCCTTTAC CAGTGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCTCCA GGTCAAGGCT GTGCGAAGTT TAACTGTGCA
    TCTGAGCAGG TGACATTCAA ACCTGGTGGC AGGAGGACGC GATTTCTGAG TACGCCCTGC
    TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TATGTGCCCT
    CCAGCTGAAC CAAAATCAGG GACCAGCAGC ACAAACACCG TTGGAGGAAC AGTGAGTAGC
    CAGGCTGCCC AGGCTCAGCC TCCTGCCATG ACTTCCAGTA GGAAGGGCAC ATTCACAGAT
    GACCTGCACA AGTTGGTAGA CAACTGGGCC CGAGATGCCA TGAATCTTTC AGGCAGGAGA
    GGCAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCTCCT
    GGGCAGCTGT GCATTTCCAT GACCTCAAAC ATGGGTGGCT CTACCCCCAT CTCTGCAGCA
    TCTGCTACCT CTCTAGGTCA CTTCACCAAG TCCATGTGCC CCCCACAGCA GTATGGTTTT
    CCAGCTGCCC CATTCGGCAC TCAGTGGAGT GGAACAGGTG GTCCAGCACC ACAGCCACTT
    GGTCAGTTCC AACCTGTAGG AACCACATCC TTGCAAAATT TCAACATCAG CAATTTGCAG
    AAGTCCATCA GCAACCCCCC AGGTTCCAAC CTACGGACCA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60699
    Clone ID Related Accession (Same CDS sequence) XM_017592392.1
    Accession Version XM_017592392.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
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    3121
    3181
    3241
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    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGGGCT TCCCATCTCG ACTGCCACCA CAATACCCAG GAGATTCAAA TATTGCTCCC
    TCTTCCAACG TGGCTTCTGT TTGCATCCAT TCTACAGTCC TAGCCCCTCC TCCTATGCCA
    ACAGAAGCAT TGGCCACCCA AGGTTACTTT CCTACAGTAG TGCAGCCTTA TGTGGAATCA
    ACTCCTTTGG TTCCTATGGG CAGTGTAGGA GGCCAGGTTC AAGTGTCCCA ACCAGCAGTG
    AGTTTATCGC AACAACCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTAGTACA
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAACCTGTTC CATTGGTTTC CTCTGTGGAC AGTGCCCATT CAGATGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCTTCATCC AGCGGGAGGC ATGAAGGGAG GACGACAAAA
    CGTCATTATC GAAAATCTGT AAGAAGTCGT TCTCGTCATG AAAAGACTTC TCGCCCGAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTGGAGACT
    CATAATCGGA AAATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCCATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GCGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTA CAAGGAAAGG ATGACTATGG CTTTCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACACAA GTTGTTCATT CTGCTGGAAG ACGATTTATA
    GTGAGTCCAG TTCCAGAAAG TCGATTACGA GAATCAAAAA TTTTCACCAG TGAAATACCA
    GATCCAGTTG CGGCCTCTAC ATCTCAGGGC CCTGGAATGA ACTTATCTCA CTCCGCTTCA
    TCCCTTAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACCCA GGACCAACAT TTTCTCCCTT TTTGACTAGC
    ATTGCTGGTG TCCAAACTGT AGCAGCATCC ACACCATCAG TATCAGTCCC TATAACAAGC
    AGCCCTCTTA ATGACATTTC CACATCAGTG ATGCAGTCAG AGGGTGCACT GCCCACTGAC
    AAAGGCATTG GGGGAGTTAC CACCAGCACA GGTGTGGTAG CTTCAGGTGG TCTCACCACA
    TTGTCTGTGT CTGAGACACC AACACTTTCC AGTGCAGTTT CAAGTTCTAC AGCGCCTGCA
    GTTGTCACAG TGTCTACAAC ATCCCAACCA GTTCAGGCTT CCACATCTGG GAGCATTGCT
    TCTAGTACAG GCAGTTTTCC TTCTGGAACA TTTTCCACAA CTACAGGTAC CACAGTGAGT
    AGTGTAGCTG TCCCAAATGC TAAGCCTCCA ACTGTATTAC TGCAACAGGT AGCAGGTAAT
    ACTGCTGGGG TAGCCATAGT GACATCAGTT TCTACTACCA CTCCATTCCC AGCCATGGCT
    TCACAGCCAT CCCTTCCACT TGGTAGCAGC ACATCTGCTC CTACACTAGC TGAAACAGTG
    GTGGTTAGTG CACACTCACT AGACAAGGCA TCTCACAGTA GTACAGCAGG ATTGGGTTTG
    TCCTTTTGCG CACCGTCCTC TTCTTCCTCC TCTGGAACAG CAGTGTCTAG TTCTGTTTCT
    CAGCCTGGTA TTGTGCATCC TTTGGTCATC TCATCAGCTA TAGCTTCTAC ACCTGTCCTC
    CCACAACCAG CAGTACCTAC TTCTACACCT TTATTACCCC AAGTACCTAA TATCCCACCC
    TTGGTACAAC CTGTTGCTAA TGTGCCTGCT GTACAGCAAA CACTCATTCA TAGTCAGCCT
    CAACCAGCTC TACTTCCCAA CCAGCCTCAC ACACACTGTC CCGAAATGGA TGCTGATACC
    CAGTCCAAAG CTCCTGGGAT CGATGATATA AAGACTCTGG AGGAAAAGCT GAGATCGCTC
    TTTAGTGAGC ATAGCTCATC TGGAACTCAG CATGCTTCTG TCTCCCTGGA GACACCCTTA
    GTAGTAGAGA CTGTCACACC GGGCATCCCT ACCACTGCTG TAGCACCAAG TAAACTCATG
    ACTTCCACTA CAAGCACTTG CTTGCCACCA ACCAATTTAC CCTTAGGAAC AGCTGGCATG
    CCAGTTATGC CAGTGGGCAC ACCTGGTCAA GTTTCTACCC CAGGCACACA TGCCTCAGCC
    CCAGCCAGCA CCGCAACAGG AGCAAAACCT GGAACAACTC CCCCAAAGCC ATCTTTAACT
    AAGACTGTGG TGCCGCCAGT GGGTACTGAG CTTTCGGCTG GTACTGTACC CTGTGAGCAG
    CTGCCACCTT TTCCCGGACC GTCTCTAATT CAGACCCAGC AACCCCTAGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTCCAG TGACACCCGC AGTTGGTCCT
    TTGTCTACGA TGTCTTCAAC GGCAGTTACA GAAGCAGGAA GTCAACCTCA GAAGGATGGC
    ACAGAAGTTC ATGTCACAGC AAGTAGCTCA GGAGCTGGTG TTGTTAAGAT GGGGAGATTT
    CAGGTTTCAG TTACAATGGA TGATGCCCAG AAAGAGCGTA AAAATAGGTC AGAAGATACA
    AAGTCGGTTC ATTTTGAATC TAGTACATCA GAGTCATCAG TTCTTTCAAG TAGTAGTCCA
    GAGAGTACCC TGGTAAAGCC AGAGCCTAAT GGAATTACTG TTTCTGGCAT CTCCTTAGAT
    GTGCCAGACA GTACCCATAG AACACCGACC CCAGAAGCAA AGTCAGAAAC AGGACAGCCT
    ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCCAACA AAGTGGGTCG TTTCTCTGTT
    TCAAGAACTG AGGACAAGGT CACAGAGCTC AAAAAAGAGG GACCAGTGAC CTCTCCTTTC
    AGAGATTCAG AACAAACTGT TATTCCTGCT GCGATACCAA AGAAGGAGAA GCCTGAGCTG
    GCAGAACCTT CCCATCTGAA TGGGCCATCT TCAGACTTGG AGGCTGCCTT TCTAAGTAGG
    GGTGGAGAGG ATGGATCAGG TAGTCCTCAC TCTCCCCCTC ACCTGTGTTC AAAGAGCCTT
    CCCATCCAGA CTCTAAGTCA AAGCCTTAGT AATTCATTCA ACTCCTCTTA CATGAGTAGT
    GACAATGAGT CCGACATTGA AGATGAAGAC TTAAGGTTAG AACTGCGACG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG
    TATACCAAAC TGGGCAAGGT TCCCCCTGCT GTCATTATTC CCCCAGCTGC TCCTCTGTCA
    GGGAGAAGAA GGAGGCCTAC TAAAAGCAAA GGCAGCAAGT CCAGTCGCAG CAGCTCATTG
    GGCAACAAAA GCCCTCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGGAAC TTCAGTCTTA
    AACCCCCAAC AGACCCTCCA TCCCCCAGGC AATACCCCAG AGACTGGACA CAACCAGCTG
    TTACAGCCTC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT TACCAGTGAT
    GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GCTGTGCGAA GTTTAACTGT
    GCATCTGAGC AGGTGACATT CAAACCTGGT GGCAGGAGGA CGCGATTTCT GAGTACGCCC
    TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA GTCTGTCCTT GCAACCAGCT CTGTATGTGC
    CCTCCAGCTG AACCAAAATC AGGGACCAGC AGCACAAACA CCGTTGGAGG AACAGTGAGT
    AGCCAGGCTG CCCAGGCTCA GCCTCCTGCC ATGACTTCCA GTAGGAAGGG CACATTCACA
    GATGACCTGC ACAAGTTGGT AGACAACTGG GCCCGAGATG CCATGAATCT TTCAGGCAGG
    AGAGGCAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCT
    CCTGGGCAGC TGTGCATTTC CATGACCTCA AACATGGGTG GCTCTACCCC CATCTCTGCA
    GCATCTGCTA CCTCTCTAGG TCACTTCACC AAGTCCATGT GCCCCCCACA GCAGTATGGT
    TTTCCAGCTG CCCCATTCGG CACTCAGTGG AGTGGAACAG GTGGTCCAGC ACCACAGCCA
    CTTGGTCAGT TCCAACCTGT AGGAACCACA TCCTTGCAAA ATTTCAACAT CAGCAATTTG
    CAGAAGTCCA TCAGCAACCC CCCAGGTTCC AACCTACGGA CCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447881.1
    CDS232..7059
    Translation

    Target ORF information:

    RefSeq Version XM_017592392.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592392.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGGGCT TCCCATCTCG ACTGCCACCA CAATACCCAG GAGATTCAAA TATTGCTCCC
    TCTTCCAACG TGGCTTCTGT TTGCATCCAT TCTACAGTCC TAGCCCCTCC TCCTATGCCA
    ACAGAAGCAT TGGCCACCCA AGGTTACTTT CCTACAGTAG TGCAGCCTTA TGTGGAATCA
    ACTCCTTTGG TTCCTATGGG CAGTGTAGGA GGCCAGGTTC AAGTGTCCCA ACCAGCAGTG
    AGTTTATCGC AACAACCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTAGTACA
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAACCTGTTC CATTGGTTTC CTCTGTGGAC AGTGCCCATT CAGATGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCTTCATCC AGCGGGAGGC ATGAAGGGAG GACGACAAAA
    CGTCATTATC GAAAATCTGT AAGAAGTCGT TCTCGTCATG AAAAGACTTC TCGCCCGAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTGGAGACT
    CATAATCGGA AAATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCCATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GCGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTA CAAGGAAAGG ATGACTATGG CTTTCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACACAA GTTGTTCATT CTGCTGGAAG ACGATTTATA
    GTGAGTCCAG TTCCAGAAAG TCGATTACGA GAATCAAAAA TTTTCACCAG TGAAATACCA
    GATCCAGTTG CGGCCTCTAC ATCTCAGGGC CCTGGAATGA ACTTATCTCA CTCCGCTTCA
    TCCCTTAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACCCA GGACCAACAT TTTCTCCCTT TTTGACTAGC
    ATTGCTGGTG TCCAAACTGT AGCAGCATCC ACACCATCAG TATCAGTCCC TATAACAAGC
    AGCCCTCTTA ATGACATTTC CACATCAGTG ATGCAGTCAG AGGGTGCACT GCCCACTGAC
    AAAGGCATTG GGGGAGTTAC CACCAGCACA GGTGTGGTAG CTTCAGGTGG TCTCACCACA
    TTGTCTGTGT CTGAGACACC AACACTTTCC AGTGCAGTTT CAAGTTCTAC AGCGCCTGCA
    GTTGTCACAG TGTCTACAAC ATCCCAACCA GTTCAGGCTT CCACATCTGG GAGCATTGCT
    TCTAGTACAG GCAGTTTTCC TTCTGGAACA TTTTCCACAA CTACAGGTAC CACAGTGAGT
    AGTGTAGCTG TCCCAAATGC TAAGCCTCCA ACTGTATTAC TGCAACAGGT AGCAGGTAAT
    ACTGCTGGGG TAGCCATAGT GACATCAGTT TCTACTACCA CTCCATTCCC AGCCATGGCT
    TCACAGCCAT CCCTTCCACT TGGTAGCAGC ACATCTGCTC CTACACTAGC TGAAACAGTG
    GTGGTTAGTG CACACTCACT AGACAAGGCA TCTCACAGTA GTACAGCAGG ATTGGGTTTG
    TCCTTTTGCG CACCGTCCTC TTCTTCCTCC TCTGGAACAG CAGTGTCTAG TTCTGTTTCT
    CAGCCTGGTA TTGTGCATCC TTTGGTCATC TCATCAGCTA TAGCTTCTAC ACCTGTCCTC
    CCACAACCAG CAGTACCTAC TTCTACACCT TTATTACCCC AAGTACCTAA TATCCCACCC
    TTGGTACAAC CTGTTGCTAA TGTGCCTGCT GTACAGCAAA CACTCATTCA TAGTCAGCCT
    CAACCAGCTC TACTTCCCAA CCAGCCTCAC ACACACTGTC CCGAAATGGA TGCTGATACC
    CAGTCCAAAG CTCCTGGGAT CGATGATATA AAGACTCTGG AGGAAAAGCT GAGATCGCTC
    TTTAGTGAGC ATAGCTCATC TGGAACTCAG CATGCTTCTG TCTCCCTGGA GACACCCTTA
    GTAGTAGAGA CTGTCACACC GGGCATCCCT ACCACTGCTG TAGCACCAAG TAAACTCATG
    ACTTCCACTA CAAGCACTTG CTTGCCACCA ACCAATTTAC CCTTAGGAAC AGCTGGCATG
    CCAGTTATGC CAGTGGGCAC ACCTGGTCAA GTTTCTACCC CAGGCACACA TGCCTCAGCC
    CCAGCCAGCA CCGCAACAGG AGCAAAACCT GGAACAACTC CCCCAAAGCC ATCTTTAACT
    AAGACTGTGG TGCCGCCAGT GGGTACTGAG CTTTCGGCTG GTACTGTACC CTGTGAGCAG
    CTGCCACCTT TTCCCGGACC GTCTCTAATT CAGACCCAGC AACCCCTAGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTCCAG TGACACCCGC AGTTGGTCCT
    TTGTCTACGA TGTCTTCAAC GGCAGTTACA GAAGCAGGAA GTCAACCTCA GAAGGATGGC
    ACAGAAGTTC ATGTCACAGC AAGTAGCTCA GGAGCTGGTG TTGTTAAGAT GGGGAGATTT
    CAGGTTTCAG TTACAATGGA TGATGCCCAG AAAGAGCGTA AAAATAGGTC AGAAGATACA
    AAGTCGGTTC ATTTTGAATC TAGTACATCA GAGTCATCAG TTCTTTCAAG TAGTAGTCCA
    GAGAGTACCC TGGTAAAGCC AGAGCCTAAT GGAATTACTG TTTCTGGCAT CTCCTTAGAT
    GTGCCAGACA GTACCCATAG AACACCGACC CCAGAAGCAA AGTCAGAAAC AGGACAGCCT
    ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCCAACA AAGTGGGTCG TTTCTCTGTT
    TCAAGAACTG AGGACAAGGT CACAGAGCTC AAAAAAGAGG GACCAGTGAC CTCTCCTTTC
    AGAGATTCAG AACAAACTGT TATTCCTGCT GCGATACCAA AGAAGGAGAA GCCTGAGCTG
    GCAGAACCTT CCCATCTGAA TGGGCCATCT TCAGACTTGG AGGCTGCCTT TCTAAGTAGG
    GGTGGAGAGG ATGGATCAGG TAGTCCTCAC TCTCCCCCTC ACCTGTGTTC AAAGAGCCTT
    CCCATCCAGA CTCTAAGTCA AAGCCTTAGT AATTCATTCA ACTCCTCTTA CATGAGTAGT
    GACAATGAGT CCGACATTGA AGATGAAGAC TTAAGGTTAG AACTGCGACG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG
    TATACCAAAC TGGGCAAGGT TCCCCCTGCT GTCATTATTC CCCCAGCTGC TCCTCTGTCA
    GGGAGAAGAA GGAGGCCTAC TAAAAGCAAA GGCAGCAAGT CCAGTCGCAG CAGCTCATTG
    GGCAACAAAA GCCCTCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGGAAC TTCAGTCTTA
    AACCCCCAAC AGACCCTCCA TCCCCCAGGC AATACCCCAG AGACTGGACA CAACCAGCTG
    TTACAGCCTC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT TACCAGTGAT
    GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GCTGTGCGAA GTTTAACTGT
    GCATCTGAGC AGGTGACATT CAAACCTGGT GGCAGGAGGA CGCGATTTCT GAGTACGCCC
    TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA GTCTGTCCTT GCAACCAGCT CTGTATGTGC
    CCTCCAGCTG AACCAAAATC AGGGACCAGC AGCACAAACA CCGTTGGAGG AACAGTGAGT
    AGCCAGGCTG CCCAGGCTCA GCCTCCTGCC ATGACTTCCA GTAGGAAGGG CACATTCACA
    GATGACCTGC ACAAGTTGGT AGACAACTGG GCCCGAGATG CCATGAATCT TTCAGGCAGG
    AGAGGCAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCT
    CCTGGGCAGC TGTGCATTTC CATGACCTCA AACATGGGTG GCTCTACCCC CATCTCTGCA
    GCATCTGCTA CCTCTCTAGG TCACTTCACC AAGTCCATGT GCCCCCCACA GCAGTATGGT
    TTTCCAGCTG CCCCATTCGG CACTCAGTGG AGTGGAACAG GTGGTCCAGC ACCACAGCCA
    CTTGGTCAGT TCCAACCTGT AGGAACCACA TCCTTGCAAA ATTTCAACAT CAGCAATTTG
    CAGAAGTCCA TCAGCAACCC CCCAGGTTCC AACCTACGGA CCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60700
    Clone ID Related Accession (Same CDS sequence) XM_017592393.1
    Accession Version XM_017592393.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGAGTA GTACACAGGG AGTCTCTCAG GCTGCTCCTC CAGAACAAAC TCCAATAACA
    CAGTCACAAC CTACTCAACC TGTTCCATTG GTTTCCTCTG TGGACAGTGC CCATTCAGAT
    GTTGCTTCTG GTATGAGTGA TGGCAATGAG AATGCTCCTT CATCCAGCGG GAGGCATGAA
    GGGAGGACGA CAAAACGTCA TTATCGAAAA TCTGTAAGAA GTCGTTCTCG TCATGAAAAG
    ACTTCTCGCC CGAAACTGAG GATTTTAAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTGG AGACTCATAA TCGGAAAATG GTTACATTTA AATTTGACCT AGATGGTGAC
    AACCCTGAAG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC CATAGAGAGA
    GAGTCTTTTG TGGCTCAAGT GCGGGAAATT ATTGAAAAGG CTGATGAAAT GTTAAGCGAG
    GATGTCAGTG TGGAGCCTGA GGGTGACCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC
    TATGGCTTTC CAGGTTCTCA GAAATTAGAA GGAGAGTTCA AGCAACCAAT TGCTGTATCT
    TCCATGCCAC AACAAATAGG TGTTCCTACC AGTTCTTTAA CACAAGTTGT TCATTCTGCT
    GGAAGACGAT TTATAGTGAG TCCAGTTCCA GAAAGTCGAT TACGAGAATC AAAAATTTTC
    ACCAGTGAAA TACCAGATCC AGTTGCGGCC TCTACATCTC AGGGCCCTGG AATGAACTTA
    TCTCACTCCG CTTCATCCCT TAGTCTCCAG CAGGCTTTTT CTGAACTGAA ACATGGTCAG
    ATGACTGAAG GACCCAATAC AGCACCTCCA AATTTTAATC ACCCAGGACC AACATTTTCT
    CCCTTTTTGA CTAGCATTGC TGGTGTCCAA ACTGTAGCAG CATCCACACC ATCAGTATCA
    GTCCCTATAA CAAGCAGCCC TCTTAATGAC ATTTCCACAT CAGTGATGCA GTCAGAGGGT
    GCACTGCCCA CTGACAAAGG CATTGGGGGA GTTACCACCA GCACAGGTGT GGTAGCTTCA
    GGTGGTCTCA CCACATTGTC TGTGTCTGAG ACACCAACAC TTTCCAGTGC AGTTTCAAGT
    TCTACAGCGC CTGCAGTTGT CACAGTGTCT ACAACATCCC AACCAGTTCA GGCTTCCACA
    TCTGGGAGCA TTGCTTCTAG TACAGGCAGT TTTCCTTCTG GAACATTTTC CACAACTACA
    GGTACCACAG TGAGTAGTGT AGCTGTCCCA AATGCTAAGC CTCCAACTGT ATTACTGCAA
    CAGGTAGCAG GTAATACTGC TGGGGTAGCC ATAGTGACAT CAGTTTCTAC TACCACTCCA
    TTCCCAGCCA TGGCTTCACA GCCATCCCTT CCACTTGGTA GCAGCACATC TGCTCCTACA
    CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCACTAGACA AGGCATCTCA CAGTAGTACA
    GCAGGATTGG GTTTGTCCTT TTGCGCACCG TCCTCTTCTT CCTCCTCTGG AACAGCAGTG
    TCTAGTTCTG TTTCTCAGCC TGGTATTGTG CATCCTTTGG TCATCTCATC AGCTATAGCT
    TCTACACCTG TCCTCCCACA ACCAGCAGTA CCTACTTCTA CACCTTTATT ACCCCAAGTA
    CCTAATATCC CACCCTTGGT ACAACCTGTT GCTAATGTGC CTGCTGTACA GCAAACACTC
    ATTCATAGTC AGCCTCAACC AGCTCTACTT CCCAACCAGC CTCACACACA CTGTCCCGAA
    ATGGATGCTG ATACCCAGTC CAAAGCTCCT GGGATCGATG ATATAAAGAC TCTGGAGGAA
    AAGCTGAGAT CGCTCTTTAG TGAGCATAGC TCATCTGGAA CTCAGCATGC TTCTGTCTCC
    CTGGAGACAC CCTTAGTAGT AGAGACTGTC ACACCGGGCA TCCCTACCAC TGCTGTAGCA
    CCAAGTAAAC TCATGACTTC CACTACAAGC ACTTGCTTGC CACCAACCAA TTTACCCTTA
    GGAACAGCTG GCATGCCAGT TATGCCAGTG GGCACACCTG GTCAAGTTTC TACCCCAGGC
    ACACATGCCT CAGCCCCAGC CAGCACCGCA ACAGGAGCAA AACCTGGAAC AACTCCCCCA
    AAGCCATCTT TAACTAAGAC TGTGGTGCCG CCAGTGGGTA CTGAGCTTTC GGCTGGTACT
    GTACCCTGTG AGCAGCTGCC ACCTTTTCCC GGACCGTCTC TAATTCAGAC CCAGCAACCC
    CTAGAAGATC TTGATGCTCA GTTGAGAAGA ACACTTAGTC CAGAGACTAT TCCAGTGACA
    CCCGCAGTTG GTCCTTTGTC TACGATGTCT TCAACGGCAG TTACAGAAGC AGGAAGTCAA
    CCTCAGAAGG ATGGCACAGA AGTTCATGTC ACAGCAAGTA GCTCAGGAGC TGGTGTTGTT
    AAGATGGGGA GATTTCAGGT TTCAGTTACA ATGGATGATG CCCAGAAAGA GCGTAAAAAT
    AGGTCAGAAG ATACAAAGTC GGTTCATTTT GAATCTAGTA CATCAGAGTC ATCAGTTCTT
    TCAAGTAGTA GTCCAGAGAG TACCCTGGTA AAGCCAGAGC CTAATGGAAT TACTGTTTCT
    GGCATCTCCT TAGATGTGCC AGACAGTACC CATAGAACAC CGACCCCAGA AGCAAAGTCA
    GAAACAGGAC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC CAACAAAGTG
    GGTCGTTTCT CTGTTTCAAG AACTGAGGAC AAGGTCACAG AGCTCAAAAA AGAGGGACCA
    GTGACCTCTC CTTTCAGAGA TTCAGAACAA ACTGTTATTC CTGCTGCGAT ACCAAAGAAG
    GAGAAGCCTG AGCTGGCAGA ACCTTCCCAT CTGAATGGGC CATCTTCAGA CTTGGAGGCT
    GCCTTTCTAA GTAGGGGTGG AGAGGATGGA TCAGGTAGTC CTCACTCTCC CCCTCACCTG
    TGTTCAAAGA GCCTTCCCAT CCAGACTCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC
    TCTTACATGA GTAGTGACAA TGAGTCCGAC ATTGAAGATG AAGACTTAAG GTTAGAACTG
    CGACGACTAC GGGAAAAACA TCTTAAAGAG ATTCAGGACC TGCAGAGTCG CCAGAAGCAT
    GAAATTGAAT CTTTGTATAC CAAACTGGGC AAGGTTCCCC CTGCTGTCAT TATTCCCCCA
    GCTGCTCCTC TGTCAGGGAG AAGAAGGAGG CCTACTAAAA GCAAAGGCAG CAAGTCCAGT
    CGCAGCAGCT CATTGGGCAA CAAAAGCCCT CAGCTTTCAG GTAACCTGTC TGGTCAGAGT
    GGAACTTCAG TCTTAAACCC CCAACAGACC CTCCATCCCC CAGGCAATAC CCCAGAGACT
    GGACACAACC AGCTGTTACA GCCTCTTAAG CCATCTCCCT CCAGTGACAA CCTCTATTCA
    GCCTTTACCA GTGATGGTGC CATTTCAGTA CCAAGCCTTT CTGCTCCAGG TCAAGGCTGT
    GCGAAGTTTA ACTGTGCATC TGAGCAGGTG ACATTCAAAC CTGGTGGCAG GAGGACGCGA
    TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC
    CAGCTCTGTA TGTGCCCTCC AGCTGAACCA AAATCAGGGA CCAGCAGCAC AAACACCGTT
    GGAGGAACAG TGAGTAGCCA GGCTGCCCAG GCTCAGCCTC CTGCCATGAC TTCCAGTAGG
    AAGGGCACAT TCACAGATGA CCTGCACAAG TTGGTAGACA ACTGGGCCCG AGATGCCATG
    AATCTTTCAG GCAGGAGAGG CAGCAAAGGA CACATGAATT ATGAGGGCCC TGGAATGGCA
    AGGAAGTTCT CTGCTCCTGG GCAGCTGTGC ATTTCCATGA CCTCAAACAT GGGTGGCTCT
    ACCCCCATCT CTGCAGCATC TGCTACCTCT CTAGGTCACT TCACCAAGTC CATGTGCCCC
    CCACAGCAGT ATGGTTTTCC AGCTGCCCCA TTCGGCACTC AGTGGAGTGG AACAGGTGGT
    CCAGCACCAC AGCCACTTGG TCAGTTCCAA CCTGTAGGAA CCACATCCTT GCAAAATTTC
    AACATCAGCA ATTTGCAGAA GTCCATCAGC AACCCCCCAG GTTCCAACCT ACGGACCACC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017447882.1
    CDS232..6774
    Translation

    Target ORF information:

    RefSeq Version XM_017592393.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017592393.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGAGTA GTACACAGGG AGTCTCTCAG GCTGCTCCTC CAGAACAAAC TCCAATAACA
    CAGTCACAAC CTACTCAACC TGTTCCATTG GTTTCCTCTG TGGACAGTGC CCATTCAGAT
    GTTGCTTCTG GTATGAGTGA TGGCAATGAG AATGCTCCTT CATCCAGCGG GAGGCATGAA
    GGGAGGACGA CAAAACGTCA TTATCGAAAA TCTGTAAGAA GTCGTTCTCG TCATGAAAAG
    ACTTCTCGCC CGAAACTGAG GATTTTAAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTGG AGACTCATAA TCGGAAAATG GTTACATTTA AATTTGACCT AGATGGTGAC
    AACCCTGAAG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC CATAGAGAGA
    GAGTCTTTTG TGGCTCAAGT GCGGGAAATT ATTGAAAAGG CTGATGAAAT GTTAAGCGAG
    GATGTCAGTG TGGAGCCTGA GGGTGACCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC
    TATGGCTTTC CAGGTTCTCA GAAATTAGAA GGAGAGTTCA AGCAACCAAT TGCTGTATCT
    TCCATGCCAC AACAAATAGG TGTTCCTACC AGTTCTTTAA CACAAGTTGT TCATTCTGCT
    GGAAGACGAT TTATAGTGAG TCCAGTTCCA GAAAGTCGAT TACGAGAATC AAAAATTTTC
    ACCAGTGAAA TACCAGATCC AGTTGCGGCC TCTACATCTC AGGGCCCTGG AATGAACTTA
    TCTCACTCCG CTTCATCCCT TAGTCTCCAG CAGGCTTTTT CTGAACTGAA ACATGGTCAG
    ATGACTGAAG GACCCAATAC AGCACCTCCA AATTTTAATC ACCCAGGACC AACATTTTCT
    CCCTTTTTGA CTAGCATTGC TGGTGTCCAA ACTGTAGCAG CATCCACACC ATCAGTATCA
    GTCCCTATAA CAAGCAGCCC TCTTAATGAC ATTTCCACAT CAGTGATGCA GTCAGAGGGT
    GCACTGCCCA CTGACAAAGG CATTGGGGGA GTTACCACCA GCACAGGTGT GGTAGCTTCA
    GGTGGTCTCA CCACATTGTC TGTGTCTGAG ACACCAACAC TTTCCAGTGC AGTTTCAAGT
    TCTACAGCGC CTGCAGTTGT CACAGTGTCT ACAACATCCC AACCAGTTCA GGCTTCCACA
    TCTGGGAGCA TTGCTTCTAG TACAGGCAGT TTTCCTTCTG GAACATTTTC CACAACTACA
    GGTACCACAG TGAGTAGTGT AGCTGTCCCA AATGCTAAGC CTCCAACTGT ATTACTGCAA
    CAGGTAGCAG GTAATACTGC TGGGGTAGCC ATAGTGACAT CAGTTTCTAC TACCACTCCA
    TTCCCAGCCA TGGCTTCACA GCCATCCCTT CCACTTGGTA GCAGCACATC TGCTCCTACA
    CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCACTAGACA AGGCATCTCA CAGTAGTACA
    GCAGGATTGG GTTTGTCCTT TTGCGCACCG TCCTCTTCTT CCTCCTCTGG AACAGCAGTG
    TCTAGTTCTG TTTCTCAGCC TGGTATTGTG CATCCTTTGG TCATCTCATC AGCTATAGCT
    TCTACACCTG TCCTCCCACA ACCAGCAGTA CCTACTTCTA CACCTTTATT ACCCCAAGTA
    CCTAATATCC CACCCTTGGT ACAACCTGTT GCTAATGTGC CTGCTGTACA GCAAACACTC
    ATTCATAGTC AGCCTCAACC AGCTCTACTT CCCAACCAGC CTCACACACA CTGTCCCGAA
    ATGGATGCTG ATACCCAGTC CAAAGCTCCT GGGATCGATG ATATAAAGAC TCTGGAGGAA
    AAGCTGAGAT CGCTCTTTAG TGAGCATAGC TCATCTGGAA CTCAGCATGC TTCTGTCTCC
    CTGGAGACAC CCTTAGTAGT AGAGACTGTC ACACCGGGCA TCCCTACCAC TGCTGTAGCA
    CCAAGTAAAC TCATGACTTC CACTACAAGC ACTTGCTTGC CACCAACCAA TTTACCCTTA
    GGAACAGCTG GCATGCCAGT TATGCCAGTG GGCACACCTG GTCAAGTTTC TACCCCAGGC
    ACACATGCCT CAGCCCCAGC CAGCACCGCA ACAGGAGCAA AACCTGGAAC AACTCCCCCA
    AAGCCATCTT TAACTAAGAC TGTGGTGCCG CCAGTGGGTA CTGAGCTTTC GGCTGGTACT
    GTACCCTGTG AGCAGCTGCC ACCTTTTCCC GGACCGTCTC TAATTCAGAC CCAGCAACCC
    CTAGAAGATC TTGATGCTCA GTTGAGAAGA ACACTTAGTC CAGAGACTAT TCCAGTGACA
    CCCGCAGTTG GTCCTTTGTC TACGATGTCT TCAACGGCAG TTACAGAAGC AGGAAGTCAA
    CCTCAGAAGG ATGGCACAGA AGTTCATGTC ACAGCAAGTA GCTCAGGAGC TGGTGTTGTT
    AAGATGGGGA GATTTCAGGT TTCAGTTACA ATGGATGATG CCCAGAAAGA GCGTAAAAAT
    AGGTCAGAAG ATACAAAGTC GGTTCATTTT GAATCTAGTA CATCAGAGTC ATCAGTTCTT
    TCAAGTAGTA GTCCAGAGAG TACCCTGGTA AAGCCAGAGC CTAATGGAAT TACTGTTTCT
    GGCATCTCCT TAGATGTGCC AGACAGTACC CATAGAACAC CGACCCCAGA AGCAAAGTCA
    GAAACAGGAC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC CAACAAAGTG
    GGTCGTTTCT CTGTTTCAAG AACTGAGGAC AAGGTCACAG AGCTCAAAAA AGAGGGACCA
    GTGACCTCTC CTTTCAGAGA TTCAGAACAA ACTGTTATTC CTGCTGCGAT ACCAAAGAAG
    GAGAAGCCTG AGCTGGCAGA ACCTTCCCAT CTGAATGGGC CATCTTCAGA CTTGGAGGCT
    GCCTTTCTAA GTAGGGGTGG AGAGGATGGA TCAGGTAGTC CTCACTCTCC CCCTCACCTG
    TGTTCAAAGA GCCTTCCCAT CCAGACTCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC
    TCTTACATGA GTAGTGACAA TGAGTCCGAC ATTGAAGATG AAGACTTAAG GTTAGAACTG
    CGACGACTAC GGGAAAAACA TCTTAAAGAG ATTCAGGACC TGCAGAGTCG CCAGAAGCAT
    GAAATTGAAT CTTTGTATAC CAAACTGGGC AAGGTTCCCC CTGCTGTCAT TATTCCCCCA
    GCTGCTCCTC TGTCAGGGAG AAGAAGGAGG CCTACTAAAA GCAAAGGCAG CAAGTCCAGT
    CGCAGCAGCT CATTGGGCAA CAAAAGCCCT CAGCTTTCAG GTAACCTGTC TGGTCAGAGT
    GGAACTTCAG TCTTAAACCC CCAACAGACC CTCCATCCCC CAGGCAATAC CCCAGAGACT
    GGACACAACC AGCTGTTACA GCCTCTTAAG CCATCTCCCT CCAGTGACAA CCTCTATTCA
    GCCTTTACCA GTGATGGTGC CATTTCAGTA CCAAGCCTTT CTGCTCCAGG TCAAGGCTGT
    GCGAAGTTTA ACTGTGCATC TGAGCAGGTG ACATTCAAAC CTGGTGGCAG GAGGACGCGA
    TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC
    CAGCTCTGTA TGTGCCCTCC AGCTGAACCA AAATCAGGGA CCAGCAGCAC AAACACCGTT
    GGAGGAACAG TGAGTAGCCA GGCTGCCCAG GCTCAGCCTC CTGCCATGAC TTCCAGTAGG
    AAGGGCACAT TCACAGATGA CCTGCACAAG TTGGTAGACA ACTGGGCCCG AGATGCCATG
    AATCTTTCAG GCAGGAGAGG CAGCAAAGGA CACATGAATT ATGAGGGCCC TGGAATGGCA
    AGGAAGTTCT CTGCTCCTGG GCAGCTGTGC ATTTCCATGA CCTCAAACAT GGGTGGCTCT
    ACCCCCATCT CTGCAGCATC TGCTACCTCT CTAGGTCACT TCACCAAGTC CATGTGCCCC
    CCACAGCAGT ATGGTTTTCC AGCTGCCCCA TTCGGCACTC AGTGGAGTGG AACAGGTGGT
    CCAGCACCAC AGCCACTTGG TCAGTTCCAA CCTGTAGGAA CCACATCCTT GCAAAATTTC
    AACATCAGCA ATTTGCAGAA GTCCATCAGC AACCCCCCAG GTTCCAACCT ACGGACCACC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa05838
    Clone ID Related Accession (Same CDS sequence) NM_001002823.2
    Accession Version NM_001002823.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF227741.1, AC106932.5 and AC106348.5. On Nov 20, 2010 this sequence version replaced NM_001002823.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This variant represents the exon combination of the dorsal root ganglia and sciatic nerve variant inferred from the mouse sequence described in Figure 2F of PubMed ID 18521183. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGCCTCAGTC CGTGGCGCAT
    CCGTGTGGGG GGACCCCAAC ATACCCAGAA TCACAGATAT TTTTCCCAAC TATTCATGAA
    CGTCCAGTTT CTTTTTCACC ACCTCCTACC TGTCCACCGA AAGTAGCCAT TTCCCAGCGA
    CGTAAGAGTA CCTCCTTCCT GGAAGCTCAA ACTCGCCACT TCCAACCCCT GCTGAGGACT
    GTTGGCCAAA ATCATCTTCC ACCTGGTGGC AGCCCAACTA ACTGGACACC AGAGGCCATA
    GTTATGTTGG GTACTACAGC CAATAGAGTA AATAGAGAGC TATGTGAGAT GCAGGTCCAA
    CCTGTGTTTG AGACAACCCA GATTTACAGT GACTATAGAC CTGGACTAGT ACTGGCAGAA
    GAAGCTCACT ATTTTATTCC TCAGGAAACA GTATATCTAG CTGGGGTGCA TTACCAGGCC
    CATGCGGCAG GACAGTATGA GGGTATTTCA TACAACTCAC CAGTACTGTC AAGTCCTATG
    AAACAGATAA CTGAACAGAA GCCAGTGCCT GGGTGCCCTG CCTCAAGTTC TGTCTTTGAA
    TTTCCTTCTG GACAGGCTTT CCTAGTAGGA CACCTTCAGA ATTTAAGATT AGATTCTGGA
    CCAAGTCCAG CATCACCCCT GTCTAGTATT TCTGCGCCTA ACAGTACAGA TGCTACACAT
    TTGAAATTTC ACCCTGTCTT TGTACCTCAT TCTGCACCAG CTGTGTTAAC TCATAGCAAT
    GAGAACAGAA GCAACTGTGT ATTTGAATTT CATGCTCAAA CACCAAGTTC CTCTTCAGGA
    GAAGGAGGTG GGATTTTACC TCAACGTGTT TACCGAAATC GACAGGTTGC AGTGGACTCA
    AGTCAGGAAG AACTGTCTCC TCAGTCAGTT GGATTACACT GCCACCTGCA GCCTGTTACT
    GAAGAACAGC GTAATAACCA TACCCCAGAA TTGACCATTT CTGTGGTAGA ACCTATGGGA
    CAAAACTGGC CAGTAGGAAG CCCAGAATAT TCCAGTGATT CCTCTCAAAT TACTTCTTCA
    GATATCAGTG ATTTTCAATC ACCTCCCCCT ACAGGGGGAA CAGCTGCACC TTTTGGCTCT
    GACGTCTCAT TGCCCTATAT TCGCCTGCCT CAGACAGTGT TACAAGAATC CCCACTATTC
    TTCTGTTTCC CCCAAGGAAC CACATCTCAG CAGGTCTTAT CTGCTTCATA TTCTTCAGGA
    GGATCTGCAC TCCATCCACA GGCACAGGGG CAGAACCAGG GCCAGCCATC AAGTAGTTTA
    GCAGGGGTTC TATCTTCACA ACCCGTCCAA CATCCTCAGC AGCAGGGAAT ACAGCCGACT
    GTACCTCCTC AACAGGCAGT ACAGTATTCA CTTCCACAGG CAGCGTCTTC CAGTGAAGGC
    ACTGTCCAGC CAGTGAGTCA GCCTCAAGTG TCAGCTGGAA CACAGGGCTT CCCATCTCGA
    CTGCCACCAC AATACCCAGG AGATTCAAAT ATTGCTCCCT CTTCCAACGT GGCTTCTGTT
    TGCATCCATT CTACAGTCCT AGCCCCTCCT CCTATGCCAA CAGAAGCATT GGCCACCCAA
    GGTTACTTTC CTACAGTAGT GCAGCCTTAT GTGGAATCAA CTCCTTTGGT TCCTATGGGC
    AGTGTAGGAG GCCAGGTTCA AGTGTCCCAA CCAGCAGTGA GTTTATCGCA ACAACCCCCC
    ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTAGTACAC AGGGAGTCTC TCAGGCTGCT
    CCTCCAGAAC AAACTCCAAT AACACAGTCA CAACCTACTC AACCTGTTCC ATTGGTTTCC
    TCTGTGGACA GTGCCCATTC AGATGTTGCT TCTGGTATGA GTGATGGCAA TGAGAATGCT
    CCTTCATCCA GCGGGAGGCA TGAAGGGAGG ACGACAAAAC GTCATTATCG AAAATCTGTA
    AGAAGTCGTT CTCGTCATGA AAAGACTTCT CGCCCGAAAC TGAGGATTTT AAATGTTTCA
    AATAAAGGAG ACCGAGTAGT AGAATGTCAA TTGGAGACTC ATAATCGGAA AATGGTTACA
    TTTAAATTTG ACCTAGATGG TGACAACCCT GAAGAGATAG CAACAATTAT GGTGAACAAT
    GACTTTATTC TAGCCATAGA GAGAGAGTCT TTTGTGGCTC AAGTGCGGGA AATTATTGAA
    AAGGCTGATG AAATGTTAAG CGAGGATGTC AGTGTGGAGC CTGAGGGTGA CCAGGGATTG
    GAGAGTCTAC AAGGAAAGGA TGACTATGGC TTTCCAGGTT CTCAGAAATT AGAAGGAGAG
    TTCAAGCAAC CAATTGCTGT ATCTTCCATG CCACAACAAA TAGGTGTTCC TACCAGTTCT
    TTAACACAAG TTGTTCATTC TGCTGGAAGA CGATTTATAG TGAGTCCAGT TCCAGAAAGT
    CGATTACGAG AATCAAAAAT TTTCACCAGT GAAATACCAG ATCCAGTTGC GGCCTCTACA
    TCTCAGGGCC CTGGAATGAA CTTATCTCAC TCCGCTTCAT CCCTTAGTCT CCAGCAGGCT
    TTTTCTGAAC TGAAACATGG TCAGATGACT GAAGGACCCA ATACAGCACC TCCAAATTTT
    AATCACCCAG GACCAACATT TTCTCCCTTT TTGACTAGCA TTGCTGGTGT CCAAACTGTA
    GCAGCATCCA CACCATCAGT ATCAGTCCCT ATAACAAGCA GCCCTCTTAA TGACATTTCC
    ACATCAGTGA TGCAGTCAGA GGGTGCACTG CCCACTGACA AAGGCATTGG GGGAGTTACC
    ACCAGCACAG GTGTGGTAGC TTCAGGTGGT CTCACCACAT TGTCTGTGTC TGAGACACCA
    ACACTTTCCA GTGCAGTTTC AAGTTCTACA GCGCCTGCAG TTGTCACAGT GTCTACAACA
    TCCCAACCAG TTCAGGCTTC CACATCTGGG AGCATTGCTT CTAGTACAGG CAGTTTTCCT
    TCTGGAACAT TTTCCACAAC TACAGGTACC ACAGTGAGTA GTGTAGCTGT CCCAAATGCT
    AAGCCTCCAA CTGTATTACT GCAACAGGTA GCAGGTAATA CTGCTGGGGT AGCCATAGTG
    ACATCAGTTT CTACTACCAC TCCATTCCCA GCCATGGCTT CACAGCCATC CCTTCCACTT
    GGTAGCAGCA CATCTGCTCC TACACTAGCT GAAACAGTGG TGGTTAGTGC ACACTCACTA
    GACAAGGCAT CTCACAGTAG TACAGCAGGA TTGGGTTTGT CCTTTTGCGC ACCGTCCTCT
    TCTTCCTCCT CTGGAACAGC AGTGTCTAGT TCTGTTTCTC AGCCTGGTAT TGTGCATCCT
    TTGGTCATCT CATCAGCTAT AGCTTCTACA CCTGTCCTCC CACAACCAGC AGTACCTACT
    TCTACACCTT TATTACCCCA AGTACCTAAT ATCCCACCCT TGGTACAACC TGTTGCTAAT
    GTGCCTGCTG TACAGCAAAC ACTCATTCAT AGTCAGCCTC AACCAGCTCT ACTTCCCAAC
    CAGCCTCACA CACACTGTCC CGAAATGGAT GCTGATACCC AGTCCAAAGC TCCTGGGATC
    GATGATATAA AGACTCTGGA GGAAAAGCTG AGATCGCTCT TTAGTGAGCA TAGCTCATCT
    GGAACTCAGC ATGCTTCTGT CTCCCTGGAG ACACCCTTAG TAGTAGAGAC TGTCACACCG
    GGCATCCCTA CCACTGCTGT AGCACCAAGT AAACTCATGA CTTCCACTAC AAGCACTTGC
    TTGCCACCAA CCAATTTACC CTTAGGAACA GCTGGCATGC CAGTTATGCC AGTGGGCACA
    CCTGGTCAAG TTTCTACCCC AGGCACACAT GCCTCAGCCC CAGCCAGCAC CGCAACAGGA
    GCAAAACCTG GAACAACTCC CCCAAAGCCA TCTTTAACTA AGACTGTGGT GCCGCCAGTG
    GGTACTGAGC TTTCGGCTGG TACTGTACCC TGTGAGCAGC TGCCACCTTT TCCCGGACCG
    TCTCTAATTC AGACCCAGCA ACCCCTAGAA GATCTTGATG CTCAGTTGAG AAGAACACTT
    AGTCCAGAGA CTATTCCAGT GACACCCGCA GTTGGTCCTT TGTCTACGAT GTCTTCAACG
    GCAGTTACAG AAGCAGGAAG TCAACCTCAG AAGGATGGCA CAGAAGTTCA TGTCACAGCA
    AGTAGCTCAG GAGCTGGTGT TGTTAAGATG GGGAGATTTC AGGTTTCAGT TACAATGGAT
    GATGCCCAGA AAGAGCGTAA AAATAGGTCA GAAGATACAA AGTCGGTTCA TTTTGAATCT
    AGTACATCAG AGTCATCAGT TCTTTCAAGT AGTAGTCCAG AGAGTACCCT GGTAAAGCCA
    GAGCCTAATG GAATTACTGT TTCTGGCATC TCCTTAGATG TGCCAGACAG TACCCATAGA
    ACACCGACCC CAGAAGCAAA GTCAGAAACA GGACAGCCTA CCAAGGTTGG ACGTTTTCAG
    GTGACAACTA CAGCCAACAA AGTGGGTCGT TTCTCTGTTT CAAGAACTGA GGACAAGGTC
    ACAGAGCTCA AAAAAGAGGG ACCAGTGACC TCTCCTTTCA GAGATTCAGA ACAAACTGTT
    ATTCCTGCTG CGATACCAAA GAAGGAGAAG CCTGAGCTGG CAGAACCTTC CCATCTGAAT
    GGGCCATCTT CAGACTTGGA GGCTGCCTTT CTAAGTAGGG GTGGAGAGGA TGGATCAGGT
    AGTCCTCACT CTCCCCCTCA CCTGTGTTCA AAGAGCCTTC CCATCCAGAC TCTAAGTCAA
    AGCCTTAGTA ATTCATTCAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC CGACATTGAA
    GATGAAGACT TAAGGTTAGA ACTGCGACGA CTACGGGAAA AACATCTTAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTT
    CCCCCTGCTG TCATTATTCC CCCAGCTGCT CCTCTGTCAG GGAGAAGAAG GAGGCCTACT
    AAAAGCAAAG GCAGCAAGTC CAGTCGCAGC AGCTCATTGG GCAACAAAAG CCCTCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGGAACT TCAGTCTTAA ACCCCCAACA GACCCTCCAT
    CCCCCAGGCA ATACCCCAGA GACTGGACAC AACCAGCTGT TACAGCCTCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTT ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG GACCAGCAGC ACAAACACCG TTGGAGGAAC AGTGAGTAGC
    CAGGCTGCCC AGGCTCAGCC TCCTGCCATG ACTTCCAGTA GGAAGGGCAC ATTCACAGAT
    GACCTGCACA AGTTGGTAGA CAACTGGGCC CGAGATGCCA TGAATCTTTC AGGCAGGAGA
    GGCAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCTCCT
    GGGCAGCTGT GCATTTCCAT GACCTCAAAC ATGGGTGGCT CTACCCCCAT CTCTGCAGCA
    TCTGCTACCT CTCTAGGTCA CTTCACCAAG TCCATGTGCC CCCCACAGCA GTATGGTTTT
    CCAGCTGCCC CATTCGGCAC TCAGTGGAGT GGAACAGGTG GTCCAGCACC ACAGCCACTT
    GGTCAGTTCC AACCTGTAGG AACCACATCC TTGCAAAATT TCAACATCAG CAATTTGCAG
    AAGTCCATCA GCAACCCCCC AGGTTCCAAC CTACGGACCA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001002823.2
    CDS166..8070
    Translation

    Target ORF information:

    RefSeq Version NM_001002823.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001002823.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGCCTCAGTC CGTGGCGCAT
    CCGTGTGGGG GGACCCCAAC ATACCCAGAA TCACAGATAT TTTTCCCAAC TATTCATGAA
    CGTCCAGTTT CTTTTTCACC ACCTCCTACC TGTCCACCGA AAGTAGCCAT TTCCCAGCGA
    CGTAAGAGTA CCTCCTTCCT GGAAGCTCAA ACTCGCCACT TCCAACCCCT GCTGAGGACT
    GTTGGCCAAA ATCATCTTCC ACCTGGTGGC AGCCCAACTA ACTGGACACC AGAGGCCATA
    GTTATGTTGG GTACTACAGC CAATAGAGTA AATAGAGAGC TATGTGAGAT GCAGGTCCAA
    CCTGTGTTTG AGACAACCCA GATTTACAGT GACTATAGAC CTGGACTAGT ACTGGCAGAA
    GAAGCTCACT ATTTTATTCC TCAGGAAACA GTATATCTAG CTGGGGTGCA TTACCAGGCC
    CATGCGGCAG GACAGTATGA GGGTATTTCA TACAACTCAC CAGTACTGTC AAGTCCTATG
    AAACAGATAA CTGAACAGAA GCCAGTGCCT GGGTGCCCTG CCTCAAGTTC TGTCTTTGAA
    TTTCCTTCTG GACAGGCTTT CCTAGTAGGA CACCTTCAGA ATTTAAGATT AGATTCTGGA
    CCAAGTCCAG CATCACCCCT GTCTAGTATT TCTGCGCCTA ACAGTACAGA TGCTACACAT
    TTGAAATTTC ACCCTGTCTT TGTACCTCAT TCTGCACCAG CTGTGTTAAC TCATAGCAAT
    GAGAACAGAA GCAACTGTGT ATTTGAATTT CATGCTCAAA CACCAAGTTC CTCTTCAGGA
    GAAGGAGGTG GGATTTTACC TCAACGTGTT TACCGAAATC GACAGGTTGC AGTGGACTCA
    AGTCAGGAAG AACTGTCTCC TCAGTCAGTT GGATTACACT GCCACCTGCA GCCTGTTACT
    GAAGAACAGC GTAATAACCA TACCCCAGAA TTGACCATTT CTGTGGTAGA ACCTATGGGA
    CAAAACTGGC CAGTAGGAAG CCCAGAATAT TCCAGTGATT CCTCTCAAAT TACTTCTTCA
    GATATCAGTG ATTTTCAATC ACCTCCCCCT ACAGGGGGAA CAGCTGCACC TTTTGGCTCT
    GACGTCTCAT TGCCCTATAT TCGCCTGCCT CAGACAGTGT TACAAGAATC CCCACTATTC
    TTCTGTTTCC CCCAAGGAAC CACATCTCAG CAGGTCTTAT CTGCTTCATA TTCTTCAGGA
    GGATCTGCAC TCCATCCACA GGCACAGGGG CAGAACCAGG GCCAGCCATC AAGTAGTTTA
    GCAGGGGTTC TATCTTCACA ACCCGTCCAA CATCCTCAGC AGCAGGGAAT ACAGCCGACT
    GTACCTCCTC AACAGGCAGT ACAGTATTCA CTTCCACAGG CAGCGTCTTC CAGTGAAGGC
    ACTGTCCAGC CAGTGAGTCA GCCTCAAGTG TCAGCTGGAA CACAGGGCTT CCCATCTCGA
    CTGCCACCAC AATACCCAGG AGATTCAAAT ATTGCTCCCT CTTCCAACGT GGCTTCTGTT
    TGCATCCATT CTACAGTCCT AGCCCCTCCT CCTATGCCAA CAGAAGCATT GGCCACCCAA
    GGTTACTTTC CTACAGTAGT GCAGCCTTAT GTGGAATCAA CTCCTTTGGT TCCTATGGGC
    AGTGTAGGAG GCCAGGTTCA AGTGTCCCAA CCAGCAGTGA GTTTATCGCA ACAACCCCCC
    ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTAGTACAC AGGGAGTCTC TCAGGCTGCT
    CCTCCAGAAC AAACTCCAAT AACACAGTCA CAACCTACTC AACCTGTTCC ATTGGTTTCC
    TCTGTGGACA GTGCCCATTC AGATGTTGCT TCTGGTATGA GTGATGGCAA TGAGAATGCT
    CCTTCATCCA GCGGGAGGCA TGAAGGGAGG ACGACAAAAC GTCATTATCG AAAATCTGTA
    AGAAGTCGTT CTCGTCATGA AAAGACTTCT CGCCCGAAAC TGAGGATTTT AAATGTTTCA
    AATAAAGGAG ACCGAGTAGT AGAATGTCAA TTGGAGACTC ATAATCGGAA AATGGTTACA
    TTTAAATTTG ACCTAGATGG TGACAACCCT GAAGAGATAG CAACAATTAT GGTGAACAAT
    GACTTTATTC TAGCCATAGA GAGAGAGTCT TTTGTGGCTC AAGTGCGGGA AATTATTGAA
    AAGGCTGATG AAATGTTAAG CGAGGATGTC AGTGTGGAGC CTGAGGGTGA CCAGGGATTG
    GAGAGTCTAC AAGGAAAGGA TGACTATGGC TTTCCAGGTT CTCAGAAATT AGAAGGAGAG
    TTCAAGCAAC CAATTGCTGT ATCTTCCATG CCACAACAAA TAGGTGTTCC TACCAGTTCT
    TTAACACAAG TTGTTCATTC TGCTGGAAGA CGATTTATAG TGAGTCCAGT TCCAGAAAGT
    CGATTACGAG AATCAAAAAT TTTCACCAGT GAAATACCAG ATCCAGTTGC GGCCTCTACA
    TCTCAGGGCC CTGGAATGAA CTTATCTCAC TCCGCTTCAT CCCTTAGTCT CCAGCAGGCT
    TTTTCTGAAC TGAAACATGG TCAGATGACT GAAGGACCCA ATACAGCACC TCCAAATTTT
    AATCACCCAG GACCAACATT TTCTCCCTTT TTGACTAGCA TTGCTGGTGT CCAAACTGTA
    GCAGCATCCA CACCATCAGT ATCAGTCCCT ATAACAAGCA GCCCTCTTAA TGACATTTCC
    ACATCAGTGA TGCAGTCAGA GGGTGCACTG CCCACTGACA AAGGCATTGG GGGAGTTACC
    ACCAGCACAG GTGTGGTAGC TTCAGGTGGT CTCACCACAT TGTCTGTGTC TGAGACACCA
    ACACTTTCCA GTGCAGTTTC AAGTTCTACA GCGCCTGCAG TTGTCACAGT GTCTACAACA
    TCCCAACCAG TTCAGGCTTC CACATCTGGG AGCATTGCTT CTAGTACAGG CAGTTTTCCT
    TCTGGAACAT TTTCCACAAC TACAGGTACC ACAGTGAGTA GTGTAGCTGT CCCAAATGCT
    AAGCCTCCAA CTGTATTACT GCAACAGGTA GCAGGTAATA CTGCTGGGGT AGCCATAGTG
    ACATCAGTTT CTACTACCAC TCCATTCCCA GCCATGGCTT CACAGCCATC CCTTCCACTT
    GGTAGCAGCA CATCTGCTCC TACACTAGCT GAAACAGTGG TGGTTAGTGC ACACTCACTA
    GACAAGGCAT CTCACAGTAG TACAGCAGGA TTGGGTTTGT CCTTTTGCGC ACCGTCCTCT
    TCTTCCTCCT CTGGAACAGC AGTGTCTAGT TCTGTTTCTC AGCCTGGTAT TGTGCATCCT
    TTGGTCATCT CATCAGCTAT AGCTTCTACA CCTGTCCTCC CACAACCAGC AGTACCTACT
    TCTACACCTT TATTACCCCA AGTACCTAAT ATCCCACCCT TGGTACAACC TGTTGCTAAT
    GTGCCTGCTG TACAGCAAAC ACTCATTCAT AGTCAGCCTC AACCAGCTCT ACTTCCCAAC
    CAGCCTCACA CACACTGTCC CGAAATGGAT GCTGATACCC AGTCCAAAGC TCCTGGGATC
    GATGATATAA AGACTCTGGA GGAAAAGCTG AGATCGCTCT TTAGTGAGCA TAGCTCATCT
    GGAACTCAGC ATGCTTCTGT CTCCCTGGAG ACACCCTTAG TAGTAGAGAC TGTCACACCG
    GGCATCCCTA CCACTGCTGT AGCACCAAGT AAACTCATGA CTTCCACTAC AAGCACTTGC
    TTGCCACCAA CCAATTTACC CTTAGGAACA GCTGGCATGC CAGTTATGCC AGTGGGCACA
    CCTGGTCAAG TTTCTACCCC AGGCACACAT GCCTCAGCCC CAGCCAGCAC CGCAACAGGA
    GCAAAACCTG GAACAACTCC CCCAAAGCCA TCTTTAACTA AGACTGTGGT GCCGCCAGTG
    GGTACTGAGC TTTCGGCTGG TACTGTACCC TGTGAGCAGC TGCCACCTTT TCCCGGACCG
    TCTCTAATTC AGACCCAGCA ACCCCTAGAA GATCTTGATG CTCAGTTGAG AAGAACACTT
    AGTCCAGAGA CTATTCCAGT GACACCCGCA GTTGGTCCTT TGTCTACGAT GTCTTCAACG
    GCAGTTACAG AAGCAGGAAG TCAACCTCAG AAGGATGGCA CAGAAGTTCA TGTCACAGCA
    AGTAGCTCAG GAGCTGGTGT TGTTAAGATG GGGAGATTTC AGGTTTCAGT TACAATGGAT
    GATGCCCAGA AAGAGCGTAA AAATAGGTCA GAAGATACAA AGTCGGTTCA TTTTGAATCT
    AGTACATCAG AGTCATCAGT TCTTTCAAGT AGTAGTCCAG AGAGTACCCT GGTAAAGCCA
    GAGCCTAATG GAATTACTGT TTCTGGCATC TCCTTAGATG TGCCAGACAG TACCCATAGA
    ACACCGACCC CAGAAGCAAA GTCAGAAACA GGACAGCCTA CCAAGGTTGG ACGTTTTCAG
    GTGACAACTA CAGCCAACAA AGTGGGTCGT TTCTCTGTTT CAAGAACTGA GGACAAGGTC
    ACAGAGCTCA AAAAAGAGGG ACCAGTGACC TCTCCTTTCA GAGATTCAGA ACAAACTGTT
    ATTCCTGCTG CGATACCAAA GAAGGAGAAG CCTGAGCTGG CAGAACCTTC CCATCTGAAT
    GGGCCATCTT CAGACTTGGA GGCTGCCTTT CTAAGTAGGG GTGGAGAGGA TGGATCAGGT
    AGTCCTCACT CTCCCCCTCA CCTGTGTTCA AAGAGCCTTC CCATCCAGAC TCTAAGTCAA
    AGCCTTAGTA ATTCATTCAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC CGACATTGAA
    GATGAAGACT TAAGGTTAGA ACTGCGACGA CTACGGGAAA AACATCTTAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTT
    CCCCCTGCTG TCATTATTCC CCCAGCTGCT CCTCTGTCAG GGAGAAGAAG GAGGCCTACT
    AAAAGCAAAG GCAGCAAGTC CAGTCGCAGC AGCTCATTGG GCAACAAAAG CCCTCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGGAACT TCAGTCTTAA ACCCCCAACA GACCCTCCAT
    CCCCCAGGCA ATACCCCAGA GACTGGACAC AACCAGCTGT TACAGCCTCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTT ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG GACCAGCAGC ACAAACACCG TTGGAGGAAC AGTGAGTAGC
    CAGGCTGCCC AGGCTCAGCC TCCTGCCATG ACTTCCAGTA GGAAGGGCAC ATTCACAGAT
    GACCTGCACA AGTTGGTAGA CAACTGGGCC CGAGATGCCA TGAATCTTTC AGGCAGGAGA
    GGCAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCTCCT
    GGGCAGCTGT GCATTTCCAT GACCTCAAAC ATGGGTGGCT CTACCCCCAT CTCTGCAGCA
    TCTGCTACCT CTCTAGGTCA CTTCACCAAG TCCATGTGCC CCCCACAGCA GTATGGTTTT
    CCAGCTGCCC CATTCGGCAC TCAGTGGAGT GGAACAGGTG GTCCAGCACC ACAGCCACTT
    GGTCAGTTCC AACCTGTAGG AACCACATCC TTGCAAAATT TCAACATCAG CAATTTGCAG
    AAGTCCATCA GCAACCCCCC AGGTTCCAAC CTACGGACCA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa08841
    Clone ID Related Accession (Same CDS sequence) NM_001199095.1
    Accession Version NM_001199095.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF227741.1, AC106932.5 and AC106348.5. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame compared to variant 1. This variant represents the exon combination of the brain and spinal cord variant inferred from the mouse sequence described in Figure 2F of PubMed ID 18521183. This variant encodes isoform 2, which is shorter than isoform 1 but has the same N- and C-termini. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGCCTCGTCG TGGCCGTAGC
    ATGTCGGTTT GTGTTCCCCA TCTTTCTGCT GTTCCCTCTC TATCCCGCAT CTCTCCCAGT
    GCTCCTTCCA CCCCACCACC AGTGCTGTCT GCACCTCTTT GTCCTTCCCT CCTTCGGAGT
    GCCCCTGAGG AAACTTTTGC GGAAAAACTC TCTAAAGCAT TGGAGAGTGT CCTGCCTATG
    CACTCTGCCT CCCAGCGCAA GCACCGACGC TCCAGCCTGC CTTCCCTCTT TGTCACTACT
    CCTCAGTCCG TGGCGCATCC GTGTGGGGGG ACCCCAACAT ACCCAGAATC ACAGATATTT
    TTCCCAACTA TTCATGAACG TCCAGTTTCT TTTTCACCAC CTCCTACCTG TCCACCGAAA
    GTAGCCATTT CCCAGCGACG TAAGAGTACC TCCTTCCTGG AAGCTCAAAC TCGCCACTTC
    CAACCCCTGC TGAGGACTGT TGGCCAAAAT CATCTTCCAC CTGGTGGCAG CCCAACTAAC
    TGGACACCAG AGGCCATAGT TATGTTGGGT ACTACAGCCA ATAGAGTAAA TAGAGAGCTA
    TGTGAGATGC AGGTCCAACC TGTGTTTGAG ACAACCCAGA TTTACAGTGA CTATAGACCT
    GGACTAGTAC TGGCAGAAGA AGCTCACTAT TTTATTCCTC AGGAAACAGT ATATCTAGCT
    GGGGTGCATT ACCAGGCCCA TGCGGCAGGA CAGTATGAGG GTATTTCATA CAACTCACCA
    GTACTGTCAA GTCCTATGAA ACAGATAACT GAACAGAAGC CAGTGCCTGG GTGCCCTGCC
    TCAAGTTCTG TCTTTGAATT TCCTTCTGGA CAGGCTTTCC TAGTAGGACA CCTTCAGAAT
    TTAAGATTAG ATTCTGGACC AAGTCCAGCA TCACCCCTGT CTAGTATTTC TGCGCCTAAC
    AGTACAGATG CTACACATTT GAAATTTCAC CCTGTCTTTG TACCTCATTC TGCACCAGCT
    GTGTTAACTC ATAGCAATGA GAACAGAAGC AACTGTGTAT TTGAATTTCA TGCTCAAACA
    CCAAGTTCCT CTTCAGGAGA AGGAGGTGGG ATTTTACCTC AACGTGTTTA CCGAAATCGA
    CAGGTTGCAG TGGACTCAAG TCAGGAAGAA CTGTCTCCTC AGTCAGTTGG ATTACACTGC
    CACCTGCAGC CTGTTACTGA AGAACAGCGT AATAACCATA CCCCAGAATT GACCATTTCT
    GTGGTAGAAC CTATGGGACA AAACTGGCCA GTAGGAAGCC CAGAATATTC CAGTGATTCC
    TCTCAAATTA CTTCTTCAGA TATCAGTGAT TTTCAATCAC CTCCCCCTAC AGGGGGAACA
    GCTGCACCTT TTGGCTCTGA CGTCTCATTG CCCTATATTC GCCTGCCTCA GACAGTGTTA
    CAAGAATCCC CACTATTCTT CTGTTTCCCC CAAGGAACCA CATCTCAGCA GGTCTTATCT
    GCTTCATATT CTTCAGGAGG ATCTGCACTC CATCCACAGG CACAGGGGCA GAACCAGGGC
    CAGCCATCAA GTAGTTTAGC AGGGGTTCTA TCTTCACAAC CCGTCCAACA TCCTCAGCAG
    CAGGGAATAC AGCCGACTGT ACCTCCTCAA CAGGCAGTAC AGTATTCACT TCCACAGGCA
    GCGTCTTCCA GTGAAGGCAC TGTCCAGCCA GTGAGTCAGC CTCAAGTGTC AGCTGGAACA
    CAGAGTAGTA CACAGGGAGT CTCTCAGGCT GCTCCTCCAG AACAAACTCC AATAACACAG
    TCACAACCTA CTCAACCTGT TCCATTGGTT TCCTCTGTGG ACAGTGCCCA TTCAGATGTT
    GCTTCTGGTA TGAGTGATGG CAATGAGAAT GCTCCTTCAT CCAGCGGGAG GCATGAAGGG
    AGGACGACAA AACGTCATTA TCGAAAATCT GTAAGAAGTC GTTCTCGTCA TGAAAAGACT
    TCTCGCCCGA AACTGAGGAT TTTAAATGTT TCAAATAAAG GAGACCGAGT AGTAGAATGT
    CAATTGGAGA CTCATAATCG GAAAATGGTT ACATTTAAAT TTGACCTAGA TGGTGACAAC
    CCTGAAGAGA TAGCAACAAT TATGGTGAAC AATGACTTTA TTCTAGCCAT AGAGAGAGAG
    TCTTTTGTGG CTCAAGTGCG GGAAATTATT GAAAAGGCTG ATGAAATGTT AAGCGAGGAT
    GTCAGTGTGG AGCCTGAGGG TGACCAGGGA TTGGAGAGTC TACAAGGAAA GGATGACTAT
    GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA GAGTTCAAGC AACCAATTGC TGTATCTTCC
    ATGCCACAAC AAATAGGTGT TCCTACCAGT TCTTTAACAC AAGTTGTTCA TTCTGCTGGA
    AGACGATTTA TAGTGAGTCC AGTTCCAGAA AGTCGATTAC GAGAATCAAA AATTTTCACC
    AGTGAAATAC CAGATCCAGT TGCGGCCTCT ACATCTCAGG GCCCTGGAAT GAACTTATCT
    CACTCCGCTT CATCCCTTAG TCTCCAGCAG GCTTTTTCTG AACTGAAACA TGGTCAGATG
    ACTGAAGGAC CCAATACAGC ACCTCCAAAT TTTAATCACC CAGGACCAAC ATTTTCTCCC
    TTTTTGACTA GCATTGCTGG TGTCCAAACT GTAGCAGCAT CCACACCATC AGTATCAGTC
    CCTATAACAA GCAGCCCTCT TAATGACATT TCCACATCAG TGATGCAGTC AGAGGGTGCA
    CTGCCCACTG ACAAAGGCAT TGGGGGAGTT ACCACCAGCA CAGGTGTGGT AGCTTCAGGT
    GGTCTCACCA CATTGTCTGT GTCTGAGACA CCAACACTTT CCAGTGCAGT TTCAAGTTCT
    ACAGCGCCTG CAGTTGTCAC AGTGTCTACA ACATCCCAAC CAGTTCAGGC TTCCACATCT
    GGGAGCATTG CTTCTAGTAC AGGCAGTTTT CCTTCTGGAA CATTTTCCAC AACTACAGGT
    ACCACAGTGA GTAGTGTAGC TGTCCCAAAT GCTAAGCCTC CAACTGTATT ACTGCAACAG
    GTAGCAGGTA ATACTGCTGG GGTAGCCATA GTGACATCAG TTTCTACTAC CACTCCATTC
    CCAGCCATGG CTTCACAGCC ATCCCTTCCA CTTGGTAGCA GCACATCTGC TCCTACACTA
    GCTGAAACAG TGGTGGTTAG TGCACACTCA CTAGACAAGG CATCTCACAG TAGTACAGCA
    GGATTGGGTT TGTCCTTTTG CGCACCGTCC TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT
    AGTTCTGTTT CTCAGCCTGG TATTGTGCAT CCTTTGGTCA TCTCATCAGC TATAGCTTCT
    ACACCTGTCC TCCCACAACC AGCAGTACCT ACTTCTACAC CTTTATTACC CCAAGTACCT
    AATATCCCAC CCTTGGTACA ACCTGTTGCT AATGTGCCTG CTGTACAGCA AACACTCATT
    CATAGTCAGC CTCAACCAGC TCTACTTCCC AACCAGCCTC ACACACACTG TCCCGAAATG
    GATGCTGATA CCCAGTCCAA AGCTCCTGGG ATCGATGATA TAAAGACTCT GGAGGAAAAG
    CTGAGATCGC TCTTTAGTGA GCATAGCTCA TCTGGAACTC AGCATGCTTC TGTCTCCCTG
    GAGACACCCT TAGTAGTAGA GACTGTCACA CCGGGCATCC CTACCACTGC TGTAGCACCA
    AGTAAACTCA TGACTTCCAC TACAAGCACT TGCTTGCCAC CAACCAATTT ACCCTTAGGA
    ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC ACACCTGGTC AAGTTTCTAC CCCAGGCACA
    CATGCCTCAG CCCCAGCCAG CACCGCAACA GGAGCAAAAC CTGGAACAAC TCCCCCAAAG
    CCATCTTTAA CTAAGACTGT GGTGCCGCCA GTGGGTACTG AGCTTTCGGC TGGTACTGTA
    CCCTGTGAGC AGCTGCCACC TTTTCCCGGA CCGTCTCTAA TTCAGACCCA GCAACCCCTA
    GAAGATCTTG ATGCTCAGTT GAGAAGAACA CTTAGTCCAG AGACTATTCC AGTGACACCC
    GCAGTTGGTC CTTTGTCTAC GATGTCTTCA ACGGCAGTTA CAGAAGCAGG AAGTCAACCT
    CAGAAGGATG GCACAGAAGT TCATGTCACA GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG
    ATGGGGAGAT TTCAGGTTTC AGTTACAATG GATGATGCCC AGAAAGAGCG TAAAAATAGG
    TCAGAAGATA CAAAGTCGGT TCATTTTGAA TCTAGTACAT CAGAGTCATC AGTTCTTTCA
    AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG CCAGAGCCTA ATGGAATTAC TGTTTCTGGC
    ATCTCCTTAG ATGTGCCAGA CAGTACCCAT AGAACACCGA CCCCAGAAGC AAAGTCAGAA
    ACAGGACAGC CTACCAAGGT TGGACGTTTT CAGGTGACAA CTACAGCCAA CAAAGTGGGT
    CGTTTCTCTG TTTCAAGAAC TGAGGACAAG GTCACAGAGC TCAAAAAAGA GGGACCAGTG
    ACCTCTCCTT TCAGAGATTC AGAACAAACT GTTATTCCTG CTGCGATACC AAAGAAGGAG
    AAGCCTGAGC TGGCAGAACC TTCCCATCTG AATGGGCCAT CTTCAGACTT GGAGGCTGCC
    TTTCTAAGTA GGGGTGGAGA GGATGGATCA GGTAGTCCTC ACTCTCCCCC TCACCTGTGT
    TCAAAGAGCC TTCCCATCCA GACTCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCCGACATT GAAGATGAAG ACTTAAGGTT AGAACTGCGA
    CGACTACGGG AAAAACATCT TAAAGAGATT CAGGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGCAAG GTTCCCCCTG CTGTCATTAT TCCCCCAGCT
    GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC
    AGCAGCTCAT TGGGCAACAA AAGCCCTCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA
    ACTTCAGTCT TAAACCCCCA ACAGACCCTC CATCCCCCAG GCAATACCCC AGAGACTGGA
    CACAACCAGC TGTTACAGCC TCTTAAGCCA TCTCCCTCCA GTGACAACCT CTATTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTACCA AGCCTTTCTG CTCCAGGTCA AGGGACCAGC
    AGCACAAACA CCGTTGGAGG AACAGTGAGT AGCCAGGCTG CCCAGGCTCA GCCTCCTGCC
    ATGACTTCCA GTAGGAAGGG CACATTCACA GATGACCTGC ACAAGTTGGT AGACAACTGG
    GCCCGAGATG CCATGAATCT TTCAGGCAGG AGAGGCAGCA AAGGACACAT GAATTATGAG
    GGCCCTGGAA TGGCAAGGAA GTTCTCTGCT CCTGGGCAGC TGTGCATTTC CATGACCTCA
    AACATGGGTG GCTCTACCCC CATCTCTGCA GCATCTGCTA CCTCTCTAGG TCACTTCACC
    AAGTCCATGT GCCCCCCACA GCAGTATGGT TTTCCAGCTG CCCCATTCGG CACTCAGTGG
    AGTGGAACAG GTGGTCCAGC ACCACAGCCA CTTGGTCAGT TCCAACCTGT AGGAACCACA
    TCCTTGCAAA ATTTCAACAT CAGCAATTTG CAGAAGTCCA TCAGCAACCC CCCAGGTTCC
    AACCTACGGA CCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186024.1
    CDS166..8043
    Translation

    Target ORF information:

    RefSeq Version NM_001199095.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199095.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGCCTCGTCG TGGCCGTAGC
    ATGTCGGTTT GTGTTCCCCA TCTTTCTGCT GTTCCCTCTC TATCCCGCAT CTCTCCCAGT
    GCTCCTTCCA CCCCACCACC AGTGCTGTCT GCACCTCTTT GTCCTTCCCT CCTTCGGAGT
    GCCCCTGAGG AAACTTTTGC GGAAAAACTC TCTAAAGCAT TGGAGAGTGT CCTGCCTATG
    CACTCTGCCT CCCAGCGCAA GCACCGACGC TCCAGCCTGC CTTCCCTCTT TGTCACTACT
    CCTCAGTCCG TGGCGCATCC GTGTGGGGGG ACCCCAACAT ACCCAGAATC ACAGATATTT
    TTCCCAACTA TTCATGAACG TCCAGTTTCT TTTTCACCAC CTCCTACCTG TCCACCGAAA
    GTAGCCATTT CCCAGCGACG TAAGAGTACC TCCTTCCTGG AAGCTCAAAC TCGCCACTTC
    CAACCCCTGC TGAGGACTGT TGGCCAAAAT CATCTTCCAC CTGGTGGCAG CCCAACTAAC
    TGGACACCAG AGGCCATAGT TATGTTGGGT ACTACAGCCA ATAGAGTAAA TAGAGAGCTA
    TGTGAGATGC AGGTCCAACC TGTGTTTGAG ACAACCCAGA TTTACAGTGA CTATAGACCT
    GGACTAGTAC TGGCAGAAGA AGCTCACTAT TTTATTCCTC AGGAAACAGT ATATCTAGCT
    GGGGTGCATT ACCAGGCCCA TGCGGCAGGA CAGTATGAGG GTATTTCATA CAACTCACCA
    GTACTGTCAA GTCCTATGAA ACAGATAACT GAACAGAAGC CAGTGCCTGG GTGCCCTGCC
    TCAAGTTCTG TCTTTGAATT TCCTTCTGGA CAGGCTTTCC TAGTAGGACA CCTTCAGAAT
    TTAAGATTAG ATTCTGGACC AAGTCCAGCA TCACCCCTGT CTAGTATTTC TGCGCCTAAC
    AGTACAGATG CTACACATTT GAAATTTCAC CCTGTCTTTG TACCTCATTC TGCACCAGCT
    GTGTTAACTC ATAGCAATGA GAACAGAAGC AACTGTGTAT TTGAATTTCA TGCTCAAACA
    CCAAGTTCCT CTTCAGGAGA AGGAGGTGGG ATTTTACCTC AACGTGTTTA CCGAAATCGA
    CAGGTTGCAG TGGACTCAAG TCAGGAAGAA CTGTCTCCTC AGTCAGTTGG ATTACACTGC
    CACCTGCAGC CTGTTACTGA AGAACAGCGT AATAACCATA CCCCAGAATT GACCATTTCT
    GTGGTAGAAC CTATGGGACA AAACTGGCCA GTAGGAAGCC CAGAATATTC CAGTGATTCC
    TCTCAAATTA CTTCTTCAGA TATCAGTGAT TTTCAATCAC CTCCCCCTAC AGGGGGAACA
    GCTGCACCTT TTGGCTCTGA CGTCTCATTG CCCTATATTC GCCTGCCTCA GACAGTGTTA
    CAAGAATCCC CACTATTCTT CTGTTTCCCC CAAGGAACCA CATCTCAGCA GGTCTTATCT
    GCTTCATATT CTTCAGGAGG ATCTGCACTC CATCCACAGG CACAGGGGCA GAACCAGGGC
    CAGCCATCAA GTAGTTTAGC AGGGGTTCTA TCTTCACAAC CCGTCCAACA TCCTCAGCAG
    CAGGGAATAC AGCCGACTGT ACCTCCTCAA CAGGCAGTAC AGTATTCACT TCCACAGGCA
    GCGTCTTCCA GTGAAGGCAC TGTCCAGCCA GTGAGTCAGC CTCAAGTGTC AGCTGGAACA
    CAGAGTAGTA CACAGGGAGT CTCTCAGGCT GCTCCTCCAG AACAAACTCC AATAACACAG
    TCACAACCTA CTCAACCTGT TCCATTGGTT TCCTCTGTGG ACAGTGCCCA TTCAGATGTT
    GCTTCTGGTA TGAGTGATGG CAATGAGAAT GCTCCTTCAT CCAGCGGGAG GCATGAAGGG
    AGGACGACAA AACGTCATTA TCGAAAATCT GTAAGAAGTC GTTCTCGTCA TGAAAAGACT
    TCTCGCCCGA AACTGAGGAT TTTAAATGTT TCAAATAAAG GAGACCGAGT AGTAGAATGT
    CAATTGGAGA CTCATAATCG GAAAATGGTT ACATTTAAAT TTGACCTAGA TGGTGACAAC
    CCTGAAGAGA TAGCAACAAT TATGGTGAAC AATGACTTTA TTCTAGCCAT AGAGAGAGAG
    TCTTTTGTGG CTCAAGTGCG GGAAATTATT GAAAAGGCTG ATGAAATGTT AAGCGAGGAT
    GTCAGTGTGG AGCCTGAGGG TGACCAGGGA TTGGAGAGTC TACAAGGAAA GGATGACTAT
    GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA GAGTTCAAGC AACCAATTGC TGTATCTTCC
    ATGCCACAAC AAATAGGTGT TCCTACCAGT TCTTTAACAC AAGTTGTTCA TTCTGCTGGA
    AGACGATTTA TAGTGAGTCC AGTTCCAGAA AGTCGATTAC GAGAATCAAA AATTTTCACC
    AGTGAAATAC CAGATCCAGT TGCGGCCTCT ACATCTCAGG GCCCTGGAAT GAACTTATCT
    CACTCCGCTT CATCCCTTAG TCTCCAGCAG GCTTTTTCTG AACTGAAACA TGGTCAGATG
    ACTGAAGGAC CCAATACAGC ACCTCCAAAT TTTAATCACC CAGGACCAAC ATTTTCTCCC
    TTTTTGACTA GCATTGCTGG TGTCCAAACT GTAGCAGCAT CCACACCATC AGTATCAGTC
    CCTATAACAA GCAGCCCTCT TAATGACATT TCCACATCAG TGATGCAGTC AGAGGGTGCA
    CTGCCCACTG ACAAAGGCAT TGGGGGAGTT ACCACCAGCA CAGGTGTGGT AGCTTCAGGT
    GGTCTCACCA CATTGTCTGT GTCTGAGACA CCAACACTTT CCAGTGCAGT TTCAAGTTCT
    ACAGCGCCTG CAGTTGTCAC AGTGTCTACA ACATCCCAAC CAGTTCAGGC TTCCACATCT
    GGGAGCATTG CTTCTAGTAC AGGCAGTTTT CCTTCTGGAA CATTTTCCAC AACTACAGGT
    ACCACAGTGA GTAGTGTAGC TGTCCCAAAT GCTAAGCCTC CAACTGTATT ACTGCAACAG
    GTAGCAGGTA ATACTGCTGG GGTAGCCATA GTGACATCAG TTTCTACTAC CACTCCATTC
    CCAGCCATGG CTTCACAGCC ATCCCTTCCA CTTGGTAGCA GCACATCTGC TCCTACACTA
    GCTGAAACAG TGGTGGTTAG TGCACACTCA CTAGACAAGG CATCTCACAG TAGTACAGCA
    GGATTGGGTT TGTCCTTTTG CGCACCGTCC TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT
    AGTTCTGTTT CTCAGCCTGG TATTGTGCAT CCTTTGGTCA TCTCATCAGC TATAGCTTCT
    ACACCTGTCC TCCCACAACC AGCAGTACCT ACTTCTACAC CTTTATTACC CCAAGTACCT
    AATATCCCAC CCTTGGTACA ACCTGTTGCT AATGTGCCTG CTGTACAGCA AACACTCATT
    CATAGTCAGC CTCAACCAGC TCTACTTCCC AACCAGCCTC ACACACACTG TCCCGAAATG
    GATGCTGATA CCCAGTCCAA AGCTCCTGGG ATCGATGATA TAAAGACTCT GGAGGAAAAG
    CTGAGATCGC TCTTTAGTGA GCATAGCTCA TCTGGAACTC AGCATGCTTC TGTCTCCCTG
    GAGACACCCT TAGTAGTAGA GACTGTCACA CCGGGCATCC CTACCACTGC TGTAGCACCA
    AGTAAACTCA TGACTTCCAC TACAAGCACT TGCTTGCCAC CAACCAATTT ACCCTTAGGA
    ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC ACACCTGGTC AAGTTTCTAC CCCAGGCACA
    CATGCCTCAG CCCCAGCCAG CACCGCAACA GGAGCAAAAC CTGGAACAAC TCCCCCAAAG
    CCATCTTTAA CTAAGACTGT GGTGCCGCCA GTGGGTACTG AGCTTTCGGC TGGTACTGTA
    CCCTGTGAGC AGCTGCCACC TTTTCCCGGA CCGTCTCTAA TTCAGACCCA GCAACCCCTA
    GAAGATCTTG ATGCTCAGTT GAGAAGAACA CTTAGTCCAG AGACTATTCC AGTGACACCC
    GCAGTTGGTC CTTTGTCTAC GATGTCTTCA ACGGCAGTTA CAGAAGCAGG AAGTCAACCT
    CAGAAGGATG GCACAGAAGT TCATGTCACA GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG
    ATGGGGAGAT TTCAGGTTTC AGTTACAATG GATGATGCCC AGAAAGAGCG TAAAAATAGG
    TCAGAAGATA CAAAGTCGGT TCATTTTGAA TCTAGTACAT CAGAGTCATC AGTTCTTTCA
    AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG CCAGAGCCTA ATGGAATTAC TGTTTCTGGC
    ATCTCCTTAG ATGTGCCAGA CAGTACCCAT AGAACACCGA CCCCAGAAGC AAAGTCAGAA
    ACAGGACAGC CTACCAAGGT TGGACGTTTT CAGGTGACAA CTACAGCCAA CAAAGTGGGT
    CGTTTCTCTG TTTCAAGAAC TGAGGACAAG GTCACAGAGC TCAAAAAAGA GGGACCAGTG
    ACCTCTCCTT TCAGAGATTC AGAACAAACT GTTATTCCTG CTGCGATACC AAAGAAGGAG
    AAGCCTGAGC TGGCAGAACC TTCCCATCTG AATGGGCCAT CTTCAGACTT GGAGGCTGCC
    TTTCTAAGTA GGGGTGGAGA GGATGGATCA GGTAGTCCTC ACTCTCCCCC TCACCTGTGT
    TCAAAGAGCC TTCCCATCCA GACTCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT
    TACATGAGTA GTGACAATGA GTCCGACATT GAAGATGAAG ACTTAAGGTT AGAACTGCGA
    CGACTACGGG AAAAACATCT TAAAGAGATT CAGGACCTGC AGAGTCGCCA GAAGCATGAA
    ATTGAATCTT TGTATACCAA ACTGGGCAAG GTTCCCCCTG CTGTCATTAT TCCCCCAGCT
    GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC
    AGCAGCTCAT TGGGCAACAA AAGCCCTCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA
    ACTTCAGTCT TAAACCCCCA ACAGACCCTC CATCCCCCAG GCAATACCCC AGAGACTGGA
    CACAACCAGC TGTTACAGCC TCTTAAGCCA TCTCCCTCCA GTGACAACCT CTATTCAGCC
    TTTACCAGTG ATGGTGCCAT TTCAGTACCA AGCCTTTCTG CTCCAGGTCA AGGGACCAGC
    AGCACAAACA CCGTTGGAGG AACAGTGAGT AGCCAGGCTG CCCAGGCTCA GCCTCCTGCC
    ATGACTTCCA GTAGGAAGGG CACATTCACA GATGACCTGC ACAAGTTGGT AGACAACTGG
    GCCCGAGATG CCATGAATCT TTCAGGCAGG AGAGGCAGCA AAGGACACAT GAATTATGAG
    GGCCCTGGAA TGGCAAGGAA GTTCTCTGCT CCTGGGCAGC TGTGCATTTC CATGACCTCA
    AACATGGGTG GCTCTACCCC CATCTCTGCA GCATCTGCTA CCTCTCTAGG TCACTTCACC
    AAGTCCATGT GCCCCCCACA GCAGTATGGT TTTCCAGCTG CCCCATTCGG CACTCAGTGG
    AGTGGAACAG GTGGTCCAGC ACCACAGCCA CTTGGTCAGT TCCAACCTGT AGGAACCACA
    TCCTTGCAAA ATTTCAACAT CAGCAATTTG CAGAAGTCCA TCAGCAACCC CCCAGGTTCC
    AACCTACGGA CCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa10663
    Clone ID Related Accession (Same CDS sequence) NM_053794.2
    Accession Version NM_053794.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF227741.1, AC106932.5 and AC106348.5. On Nov 20, 2010 this sequence version replaced NM_053794.1. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame compared to variant 1. The resulting protein (isoform 3) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF227741.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGAGTA GTACACAGGG AGTCTCTCAG GCTGCTCCTC CAGAACAAAC TCCAATAACA
    CAGTCACAAC CTACTCAACC TGTTCCATTG GTTTCCTCTG TGGACAGTGC CCATTCAGAT
    GTTGCTTCTG GTATGAGTGA TGGCAATGAG AATGCTCCTT CATCCAGCGG GAGGCATGAA
    GGGAGGACGA CAAAACGTCA TTATCGAAAA TCTGTAAGAA GTCGTTCTCG TCATGAAAAG
    ACTTCTCGCC CGAAACTGAG GATTTTAAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTGG AGACTCATAA TCGGAAAATG GTTACATTTA AATTTGACCT AGATGGTGAC
    AACCCTGAAG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC CATAGAGAGA
    GAGTCTTTTG TGGCTCAAGT GCGGGAAATT ATTGAAAAGG CTGATGAAAT GTTAAGCGAG
    GATGTCAGTG TGGAGCCTGA GGGTGACCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC
    TATGGCTTTC CAGGTTCTCA GAAATTAGAA GGAGAGTTCA AGCAACCAAT TGCTGTATCT
    TCCATGCCAC AACAAATAGG TGTTCCTACC AGTTCTTTAA CACAAGTTGT TCATTCTGCT
    GGAAGACGAT TTATAGTGAG TCCAGTTCCA GAAAGTCGAT TACGAGAATC AAAAATTTTC
    ACCAGTGAAA TACCAGATCC AGTTGCGGCC TCTACATCTC AGGGCCCTGG AATGAACTTA
    TCTCACTCCG CTTCATCCCT TAGTCTCCAG CAGGCTTTTT CTGAACTGAA ACATGGTCAG
    ATGACTGAAG GACCCAATAC AGCACCTCCA AATTTTAATC ACCCAGGACC AACATTTTCT
    CCCTTTTTGA CTAGCATTGC TGGTGTCCAA ACTGTAGCAG CATCCACACC ATCAGTATCA
    GTCCCTATAA CAAGCAGCCC TCTTAATGAC ATTTCCACAT CAGTGATGCA GTCAGAGGGT
    GCACTGCCCA CTGACAAAGG CATTGGGGGA GTTACCACCA GCACAGGTGT GGTAGCTTCA
    GGTGGTCTCA CCACATTGTC TGTGTCTGAG ACACCAACAC TTTCCAGTGC AGTTTCAAGT
    TCTACAGCGC CTGCAGTTGT CACAGTGTCT ACAACATCCC AACCAGTTCA GGCTTCCACA
    TCTGGGAGCA TTGCTTCTAG TACAGGCAGT TTTCCTTCTG GAACATTTTC CACAACTACA
    GGTACCACAG TGAGTAGTGT AGCTGTCCCA AATGCTAAGC CTCCAACTGT ATTACTGCAA
    CAGGTAGCAG GTAATACTGC TGGGGTAGCC ATAGTGACAT CAGTTTCTAC TACCACTCCA
    TTCCCAGCCA TGGCTTCACA GCCATCCCTT CCACTTGGTA GCAGCACATC TGCTCCTACA
    CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCACTAGACA AGGCATCTCA CAGTAGTACA
    GCAGGATTGG GTTTGTCCTT TTGCGCACCG TCCTCTTCTT CCTCCTCTGG AACAGCAGTG
    TCTAGTTCTG TTTCTCAGCC TGGTATTGTG CATCCTTTGG TCATCTCATC AGCTATAGCT
    TCTACACCTG TCCTCCCACA ACCAGCAGTA CCTACTTCTA CACCTTTATT ACCCCAAGTA
    CCTAATATCC CACCCTTGGT ACAACCTGTT GCTAATGTGC CTGCTGTACA GCAAACACTC
    ATTCATAGTC AGCCTCAACC AGCTCTACTT CCCAACCAGC CTCACACACA CTGTCCCGAA
    ATGGATGCTG ATACCCAGTC CAAAGCTCCT GGGATCGATG ATATAAAGAC TCTGGAGGAA
    AAGCTGAGAT CGCTCTTTAG TGAGCATAGC TCATCTGGAA CTCAGCATGC TTCTGTCTCC
    CTGGAGACAC CCTTAGTAGT AGAGACTGTC ACACCGGGCA TCCCTACCAC TGCTGTAGCA
    CCAAGTAAAC TCATGACTTC CACTACAAGC ACTTGCTTGC CACCAACCAA TTTACCCTTA
    GGAACAGCTG GCATGCCAGT TATGCCAGTG GGCACACCTG GTCAAGTTTC TACCCCAGGC
    ACACATGCCT CAGCCCCAGC CAGCACCGCA ACAGGAGCAA AACCTGGAAC AACTCCCCCA
    AAGCCATCTT TAACTAAGAC TGTGGTGCCG CCAGTGGGTA CTGAGCTTTC GGCTGGTACT
    GTACCCTGTG AGCAGCTGCC ACCTTTTCCC GGACCGTCTC TAATTCAGAC CCAGCAACCC
    CTAGAAGATC TTGATGCTCA GTTGAGAAGA ACACTTAGTC CAGAGACTAT TCCAGTGACA
    CCCGCAGTTG GTCCTTTGTC TACGATGTCT TCAACGGCAG TTACAGAAGC AGGAAGTCAA
    CCTCAGAAGG ATGGCACAGA AGTTCATGTC ACAGCAAGTA GCTCAGGAGC TGGTGTTGTT
    AAGATGGGGA GATTTCAGGT TTCAGTTACA ATGGATGATG CCCAGAAAGA GCGTAAAAAT
    AGGTCAGAAG ATACAAAGTC GGTTCATTTT GAATCTAGTA CATCAGAGTC ATCAGTTCTT
    TCAAGTAGTA GTCCAGAGAG TACCCTGGTA AAGCCAGAGC CTAATGGAAT TACTGTTTCT
    GGCATCTCCT TAGATGTGCC AGACAGTACC CATAGAACAC CGACCCCAGA AGCAAAGTCA
    GAAACAGGAC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC CAACAAAGTG
    GGTCGTTTCT CTGTTTCAAG AACTGAGGAC AAGGTCACAG AGCTCAAAAA AGAGGGACCA
    GTGACCTCTC CTTTCAGAGA TTCAGAACAA ACTGTTATTC CTGCTGCGAT ACCAAAGAAG
    GAGAAGCCTG AGCTGGCAGA ACCTTCCCAT CTGAATGGGC CATCTTCAGA CTTGGAGGCT
    GCCTTTCTAA GTAGGGGTGG AGAGGATGGA TCAGGTAGTC CTCACTCTCC CCCTCACCTG
    TGTTCAAAGA GCCTTCCCAT CCAGACTCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC
    TCTTACATGA GTAGTGACAA TGAGTCCGAC ATTGAAGATG AAGACTTAAG GTTAGAACTG
    CGACGACTAC GGGAAAAACA TCTTAAAGAG ATTCAGGACC TGCAGAGTCG CCAGAAGCAT
    GAAATTGAAT CTTTGTATAC CAAACTGGGC AAGGTTCCCC CTGCTGTCAT TATTCCCCCA
    GCTGCTCCTC TGTCAGGGAG AAGAAGGAGG CCTACTAAAA GCAAAGGCAG CAAGTCCAGT
    CGCAGCAGCT CATTGGGCAA CAAAAGCCCT CAGCTTTCAG GTAACCTGTC TGGTCAGAGT
    GGAACTTCAG TCTTAAACCC CCAACAGACC CTCCATCCCC CAGGCAATAC CCCAGAGACT
    GGACACAACC AGCTGTTACA GCCTCTTAAG CCATCTCCCT CCAGTGACAA CCTCTATTCA
    GCCTTTACCA GTGATGGTGC CATTTCAGTA CCAAGCCTTT CTGCTCCAGG TCAAGGGACC
    AGCAGCACAA ACACCGTTGG AGGAACAGTG AGTAGCCAGG CTGCCCAGGC TCAGCCTCCT
    GCCATGACTT CCAGTAGGAA GGGCACATTC ACAGATGACC TGCACAAGTT GGTAGACAAC
    TGGGCCCGAG ATGCCATGAA TCTTTCAGGC AGGAGAGGCA GCAAAGGACA CATGAATTAT
    GAGGGCCCTG GAATGGCAAG GAAGTTCTCT GCTCCTGGGC AGCTGTGCAT TTCCATGACC
    TCAAACATGG GTGGCTCTAC CCCCATCTCT GCAGCATCTG CTACCTCTCT AGGTCACTTC
    ACCAAGTCCA TGTGCCCCCC ACAGCAGTAT GGTTTTCCAG CTGCCCCATT CGGCACTCAG
    TGGAGTGGAA CAGGTGGTCC AGCACCACAG CCACTTGGTC AGTTCCAACC TGTAGGAACC
    ACATCCTTGC AAAATTTCAA CATCAGCAAT TTGCAGAAGT CCATCAGCAA CCCCCCAGGT
    TCCAACCTAC GGACCACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_446246.2
    CDS166..6546
    Translation

    Target ORF information:

    RefSeq Version NM_053794.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053794.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGAGTA GTACACAGGG AGTCTCTCAG GCTGCTCCTC CAGAACAAAC TCCAATAACA
    CAGTCACAAC CTACTCAACC TGTTCCATTG GTTTCCTCTG TGGACAGTGC CCATTCAGAT
    GTTGCTTCTG GTATGAGTGA TGGCAATGAG AATGCTCCTT CATCCAGCGG GAGGCATGAA
    GGGAGGACGA CAAAACGTCA TTATCGAAAA TCTGTAAGAA GTCGTTCTCG TCATGAAAAG
    ACTTCTCGCC CGAAACTGAG GATTTTAAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTGG AGACTCATAA TCGGAAAATG GTTACATTTA AATTTGACCT AGATGGTGAC
    AACCCTGAAG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC CATAGAGAGA
    GAGTCTTTTG TGGCTCAAGT GCGGGAAATT ATTGAAAAGG CTGATGAAAT GTTAAGCGAG
    GATGTCAGTG TGGAGCCTGA GGGTGACCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC
    TATGGCTTTC CAGGTTCTCA GAAATTAGAA GGAGAGTTCA AGCAACCAAT TGCTGTATCT
    TCCATGCCAC AACAAATAGG TGTTCCTACC AGTTCTTTAA CACAAGTTGT TCATTCTGCT
    GGAAGACGAT TTATAGTGAG TCCAGTTCCA GAAAGTCGAT TACGAGAATC AAAAATTTTC
    ACCAGTGAAA TACCAGATCC AGTTGCGGCC TCTACATCTC AGGGCCCTGG AATGAACTTA
    TCTCACTCCG CTTCATCCCT TAGTCTCCAG CAGGCTTTTT CTGAACTGAA ACATGGTCAG
    ATGACTGAAG GACCCAATAC AGCACCTCCA AATTTTAATC ACCCAGGACC AACATTTTCT
    CCCTTTTTGA CTAGCATTGC TGGTGTCCAA ACTGTAGCAG CATCCACACC ATCAGTATCA
    GTCCCTATAA CAAGCAGCCC TCTTAATGAC ATTTCCACAT CAGTGATGCA GTCAGAGGGT
    GCACTGCCCA CTGACAAAGG CATTGGGGGA GTTACCACCA GCACAGGTGT GGTAGCTTCA
    GGTGGTCTCA CCACATTGTC TGTGTCTGAG ACACCAACAC TTTCCAGTGC AGTTTCAAGT
    TCTACAGCGC CTGCAGTTGT CACAGTGTCT ACAACATCCC AACCAGTTCA GGCTTCCACA
    TCTGGGAGCA TTGCTTCTAG TACAGGCAGT TTTCCTTCTG GAACATTTTC CACAACTACA
    GGTACCACAG TGAGTAGTGT AGCTGTCCCA AATGCTAAGC CTCCAACTGT ATTACTGCAA
    CAGGTAGCAG GTAATACTGC TGGGGTAGCC ATAGTGACAT CAGTTTCTAC TACCACTCCA
    TTCCCAGCCA TGGCTTCACA GCCATCCCTT CCACTTGGTA GCAGCACATC TGCTCCTACA
    CTAGCTGAAA CAGTGGTGGT TAGTGCACAC TCACTAGACA AGGCATCTCA CAGTAGTACA
    GCAGGATTGG GTTTGTCCTT TTGCGCACCG TCCTCTTCTT CCTCCTCTGG AACAGCAGTG
    TCTAGTTCTG TTTCTCAGCC TGGTATTGTG CATCCTTTGG TCATCTCATC AGCTATAGCT
    TCTACACCTG TCCTCCCACA ACCAGCAGTA CCTACTTCTA CACCTTTATT ACCCCAAGTA
    CCTAATATCC CACCCTTGGT ACAACCTGTT GCTAATGTGC CTGCTGTACA GCAAACACTC
    ATTCATAGTC AGCCTCAACC AGCTCTACTT CCCAACCAGC CTCACACACA CTGTCCCGAA
    ATGGATGCTG ATACCCAGTC CAAAGCTCCT GGGATCGATG ATATAAAGAC TCTGGAGGAA
    AAGCTGAGAT CGCTCTTTAG TGAGCATAGC TCATCTGGAA CTCAGCATGC TTCTGTCTCC
    CTGGAGACAC CCTTAGTAGT AGAGACTGTC ACACCGGGCA TCCCTACCAC TGCTGTAGCA
    CCAAGTAAAC TCATGACTTC CACTACAAGC ACTTGCTTGC CACCAACCAA TTTACCCTTA
    GGAACAGCTG GCATGCCAGT TATGCCAGTG GGCACACCTG GTCAAGTTTC TACCCCAGGC
    ACACATGCCT CAGCCCCAGC CAGCACCGCA ACAGGAGCAA AACCTGGAAC AACTCCCCCA
    AAGCCATCTT TAACTAAGAC TGTGGTGCCG CCAGTGGGTA CTGAGCTTTC GGCTGGTACT
    GTACCCTGTG AGCAGCTGCC ACCTTTTCCC GGACCGTCTC TAATTCAGAC CCAGCAACCC
    CTAGAAGATC TTGATGCTCA GTTGAGAAGA ACACTTAGTC CAGAGACTAT TCCAGTGACA
    CCCGCAGTTG GTCCTTTGTC TACGATGTCT TCAACGGCAG TTACAGAAGC AGGAAGTCAA
    CCTCAGAAGG ATGGCACAGA AGTTCATGTC ACAGCAAGTA GCTCAGGAGC TGGTGTTGTT
    AAGATGGGGA GATTTCAGGT TTCAGTTACA ATGGATGATG CCCAGAAAGA GCGTAAAAAT
    AGGTCAGAAG ATACAAAGTC GGTTCATTTT GAATCTAGTA CATCAGAGTC ATCAGTTCTT
    TCAAGTAGTA GTCCAGAGAG TACCCTGGTA AAGCCAGAGC CTAATGGAAT TACTGTTTCT
    GGCATCTCCT TAGATGTGCC AGACAGTACC CATAGAACAC CGACCCCAGA AGCAAAGTCA
    GAAACAGGAC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC CAACAAAGTG
    GGTCGTTTCT CTGTTTCAAG AACTGAGGAC AAGGTCACAG AGCTCAAAAA AGAGGGACCA
    GTGACCTCTC CTTTCAGAGA TTCAGAACAA ACTGTTATTC CTGCTGCGAT ACCAAAGAAG
    GAGAAGCCTG AGCTGGCAGA ACCTTCCCAT CTGAATGGGC CATCTTCAGA CTTGGAGGCT
    GCCTTTCTAA GTAGGGGTGG AGAGGATGGA TCAGGTAGTC CTCACTCTCC CCCTCACCTG
    TGTTCAAAGA GCCTTCCCAT CCAGACTCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC
    TCTTACATGA GTAGTGACAA TGAGTCCGAC ATTGAAGATG AAGACTTAAG GTTAGAACTG
    CGACGACTAC GGGAAAAACA TCTTAAAGAG ATTCAGGACC TGCAGAGTCG CCAGAAGCAT
    GAAATTGAAT CTTTGTATAC CAAACTGGGC AAGGTTCCCC CTGCTGTCAT TATTCCCCCA
    GCTGCTCCTC TGTCAGGGAG AAGAAGGAGG CCTACTAAAA GCAAAGGCAG CAAGTCCAGT
    CGCAGCAGCT CATTGGGCAA CAAAAGCCCT CAGCTTTCAG GTAACCTGTC TGGTCAGAGT
    GGAACTTCAG TCTTAAACCC CCAACAGACC CTCCATCCCC CAGGCAATAC CCCAGAGACT
    GGACACAACC AGCTGTTACA GCCTCTTAAG CCATCTCCCT CCAGTGACAA CCTCTATTCA
    GCCTTTACCA GTGATGGTGC CATTTCAGTA CCAAGCCTTT CTGCTCCAGG TCAAGGGACC
    AGCAGCACAA ACACCGTTGG AGGAACAGTG AGTAGCCAGG CTGCCCAGGC TCAGCCTCCT
    GCCATGACTT CCAGTAGGAA GGGCACATTC ACAGATGACC TGCACAAGTT GGTAGACAAC
    TGGGCCCGAG ATGCCATGAA TCTTTCAGGC AGGAGAGGCA GCAAAGGACA CATGAATTAT
    GAGGGCCCTG GAATGGCAAG GAAGTTCTCT GCTCCTGGGC AGCTGTGCAT TTCCATGACC
    TCAAACATGG GTGGCTCTAC CCCCATCTCT GCAGCATCTG CTACCTCTCT AGGTCACTTC
    ACCAAGTCCA TGTGCCCCCC ACAGCAGTAT GGTTTTCCAG CTGCCCCATT CGGCACTCAG
    TGGAGTGGAA CAGGTGGTCC AGCACCACAG CCACTTGGTC AGTTCCAACC TGTAGGAACC
    ACATCCTTGC AAAATTTCAA CATCAGCAAT TTGCAGAAGT CCATCAGCAA CCCCCCAGGT
    TCCAACCTAC GGACCACCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43975
    Clone ID Related Accession (Same CDS sequence) XM_008763201.2
    Accession Version XM_008763201.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7263bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763201.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG AAGTTTAACT GTGCATCTGA GCAGGTGACA
    TTCAAACCTG GTGGCAGGAG GACGCGATTT CTGAGTACGC CCTGCTTGGC TCTTTGGAAG
    ATGGTGAAAA AAGTCTGTCC TTGCAACCAG CTCTGTAGGA CCAGCAGCAC AAACACCGTT
    GGAGGAACAG TGAGTAGCCA GGCTGCCCAG GCTCAGCCTC CTGCCATGAC TTCCAGTAGG
    AAGGGCACAT TCACAGATGA CCTGCACAAG TTGGTAGACA ACTGGGCCCG AGATGCCATG
    AATCTTTCAG GCAGGAGAGG CAGCAAAGGA CACATGAATT ATGAGGGCCC TGGAATGGCA
    AGGAAGTTCT CTGCTCCTGG GCAGCTGTGC ATTTCCATGA CCTCAAACAT GGGTGGCTCT
    ACCCCCATCT CTGCAGCATC TGCTACCTCT CTAGGTCACT TCACCAAGTC CATGTGCCCC
    CCACAGCAGT ATGGTTTTCC AGCTGCCCCA TTCGGCACTC AGTGGAGTGG AACAGGTGGT
    CCAGCACCAC AGCCACTTGG TCAGTTCCAA CCTGTAGGAA CCACATCCTT GCAAAATTTC
    AACATCAGCA ATTTGCAGAA GTCCATCAGC AACCCCCCAG GTTCCAACCT ACGGACCACC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008761423.1
    CDS232..7494
    Translation

    Target ORF information:

    RefSeq Version XM_008763201.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008763201.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG AAGTTTAACT GTGCATCTGA GCAGGTGACA
    TTCAAACCTG GTGGCAGGAG GACGCGATTT CTGAGTACGC CCTGCTTGGC TCTTTGGAAG
    ATGGTGAAAA AAGTCTGTCC TTGCAACCAG CTCTGTAGGA CCAGCAGCAC AAACACCGTT
    GGAGGAACAG TGAGTAGCCA GGCTGCCCAG GCTCAGCCTC CTGCCATGAC TTCCAGTAGG
    AAGGGCACAT TCACAGATGA CCTGCACAAG TTGGTAGACA ACTGGGCCCG AGATGCCATG
    AATCTTTCAG GCAGGAGAGG CAGCAAAGGA CACATGAATT ATGAGGGCCC TGGAATGGCA
    AGGAAGTTCT CTGCTCCTGG GCAGCTGTGC ATTTCCATGA CCTCAAACAT GGGTGGCTCT
    ACCCCCATCT CTGCAGCATC TGCTACCTCT CTAGGTCACT TCACCAAGTC CATGTGCCCC
    CCACAGCAGT ATGGTTTTCC AGCTGCCCCA TTCGGCACTC AGTGGAGTGG AACAGGTGGT
    CCAGCACCAC AGCCACTTGG TCAGTTCCAA CCTGTAGGAA CCACATCCTT GCAAAATTTC
    AACATCAGCA ATTTGCAGAA GTCCATCAGC AACCCCCCAG GTTCCAACCT ACGGACCACC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43977
    Clone ID Related Accession (Same CDS sequence) XM_008763203.2
    Accession Version XM_008763203.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763203.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG AAGTTTAACT GTGCATCTGA GCAGGTGACA
    TTCAAACCTG GTGGCAGGAG GACGCGATTT CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT
    TGCAACCAGC TCTGTAGGAC CAGCAGCACA AACACCGTTG GAGGAACAGT GAGTAGCCAG
    GCTGCCCAGG CTCAGCCTCC TGCCATGACT TCCAGTAGGA AGGGCACATT CACAGATGAC
    CTGCACAAGT TGGTAGACAA CTGGGCCCGA GATGCCATGA ATCTTTCAGG CAGGAGAGGC
    AGCAAAGGAC ACATGAATTA TGAGGGCCCT GGAATGGCAA GGAAGTTCTC TGCTCCTGGG
    CAGCTGTGCA TTTCCATGAC CTCAAACATG GGTGGCTCTA CCCCCATCTC TGCAGCATCT
    GCTACCTCTC TAGGTCACTT CACCAAGTCC ATGTGCCCCC CACAGCAGTA TGGTTTTCCA
    GCTGCCCCAT TCGGCACTCA GTGGAGTGGA ACAGGTGGTC CAGCACCACA GCCACTTGGT
    CAGTTCCAAC CTGTAGGAAC CACATCCTTG CAAAATTTCA ACATCAGCAA TTTGCAGAAG
    TCCATCAGCA ACCCCCCAGG TTCCAACCTA CGGACCACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008761425.1
    CDS232..7473
    Translation

    Target ORF information:

    RefSeq Version XM_008763203.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008763203.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG AAGTTTAACT GTGCATCTGA GCAGGTGACA
    TTCAAACCTG GTGGCAGGAG GACGCGATTT CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT
    TGCAACCAGC TCTGTAGGAC CAGCAGCACA AACACCGTTG GAGGAACAGT GAGTAGCCAG
    GCTGCCCAGG CTCAGCCTCC TGCCATGACT TCCAGTAGGA AGGGCACATT CACAGATGAC
    CTGCACAAGT TGGTAGACAA CTGGGCCCGA GATGCCATGA ATCTTTCAGG CAGGAGAGGC
    AGCAAAGGAC ACATGAATTA TGAGGGCCCT GGAATGGCAA GGAAGTTCTC TGCTCCTGGG
    CAGCTGTGCA TTTCCATGAC CTCAAACATG GGTGGCTCTA CCCCCATCTC TGCAGCATCT
    GCTACCTCTC TAGGTCACTT CACCAAGTCC ATGTGCCCCC CACAGCAGTA TGGTTTTCCA
    GCTGCCCCAT TCGGCACTCA GTGGAGTGGA ACAGGTGGTC CAGCACCACA GCCACTTGGT
    CAGTTCCAAC CTGTAGGAAC CACATCCTTG CAAAATTTCA ACATCAGCAA TTTGCAGAAG
    TCCATCAGCA ACCCCCCAGG TTCCAACCTA CGGACCACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43979
    Clone ID Related Accession (Same CDS sequence) XM_008763205.2
    Accession Version XM_008763205.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763205.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGGAAGATG GTGAAAAAAG TCTGTCCTTG CAACCAGCTC
    TGTAGGACCA GCAGCACAAA CACCGTTGGA GGAACAGTGA GTAGCCAGGC TGCCCAGGCT
    CAGCCTCCTG CCATGACTTC CAGTAGGAAG GGCACATTCA CAGATGACCT GCACAAGTTG
    GTAGACAACT GGGCCCGAGA TGCCATGAAT CTTTCAGGCA GGAGAGGCAG CAAAGGACAC
    ATGAATTATG AGGGCCCTGG AATGGCAAGG AAGTTCTCTG CTCCTGGGCA GCTGTGCATT
    TCCATGACCT CAAACATGGG TGGCTCTACC CCCATCTCTG CAGCATCTGC TACCTCTCTA
    GGTCACTTCA CCAAGTCCAT GTGCCCCCCA CAGCAGTATG GTTTTCCAGC TGCCCCATTC
    GGCACTCAGT GGAGTGGAAC AGGTGGTCCA GCACCACAGC CACTTGGTCA GTTCCAACCT
    GTAGGAACCA CATCCTTGCA AAATTTCAAC ATCAGCAATT TGCAGAAGTC CATCAGCAAC
    CCCCCAGGTT CCAACCTACG GACCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008761427.1
    CDS232..7401
    Translation

    Target ORF information:

    RefSeq Version XM_008763205.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008763205.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGGAAGATG GTGAAAAAAG TCTGTCCTTG CAACCAGCTC
    TGTAGGACCA GCAGCACAAA CACCGTTGGA GGAACAGTGA GTAGCCAGGC TGCCCAGGCT
    CAGCCTCCTG CCATGACTTC CAGTAGGAAG GGCACATTCA CAGATGACCT GCACAAGTTG
    GTAGACAACT GGGCCCGAGA TGCCATGAAT CTTTCAGGCA GGAGAGGCAG CAAAGGACAC
    ATGAATTATG AGGGCCCTGG AATGGCAAGG AAGTTCTCTG CTCCTGGGCA GCTGTGCATT
    TCCATGACCT CAAACATGGG TGGCTCTACC CCCATCTCTG CAGCATCTGC TACCTCTCTA
    GGTCACTTCA CCAAGTCCAT GTGCCCCCCA CAGCAGTATG GTTTTCCAGC TGCCCCATTC
    GGCACTCAGT GGAGTGGAAC AGGTGGTCCA GCACCACAGC CACTTGGTCA GTTCCAACCT
    GTAGGAACCA CATCCTTGCA AAATTTCAAC ATCAGCAATT TGCAGAAGTC CATCAGCAAC
    CCCCCAGGTT CCAACCTACG GACCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43981
    Clone ID Related Accession (Same CDS sequence) XM_008763207.2
    Accession Version XM_008763207.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763207.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGGACCAGC AGCACAAACA CCGTTGGAGG AACAGTGAGT
    AGCCAGGCTG CCCAGGCTCA GCCTCCTGCC ATGACTTCCA GTAGGAAGGG CACATTCACA
    GATGACCTGC ACAAGTTGGT AGACAACTGG GCCCGAGATG CCATGAATCT TTCAGGCAGG
    AGAGGCAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCT
    CCTGGGCAGC TGTGCATTTC CATGACCTCA AACATGGGTG GCTCTACCCC CATCTCTGCA
    GCATCTGCTA CCTCTCTAGG TCACTTCACC AAGTCCATGT GCCCCCCACA GCAGTATGGT
    TTTCCAGCTG CCCCATTCGG CACTCAGTGG AGTGGAACAG GTGGTCCAGC ACCACAGCCA
    CTTGGTCAGT TCCAACCTGT AGGAACCACA TCCTTGCAAA ATTTCAACAT CAGCAATTTG
    CAGAAGTCCA TCAGCAACCC CCCAGGTTCC AACCTACGGA CCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008761429.1
    CDS232..7359
    Translation

    Target ORF information:

    RefSeq Version XM_008763207.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008763207.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGCTTC CAGTTTCCCA GATAGTACCA ACTGTCCAAG GTGAACCTCA CATCCCAGTT
    TCAACACAAC CCTCAGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC TATGGGACAG
    CCAATCCCTA CTTCTTTACT CCCACAGTAT CCTGTCTCTC AAATTTCCAT ATCGACTCCT
    CATGTGTCCA CGGCGCAAAC AGGTTTCTCA TCTGTTCCTA TAACAATGGC TGCTGGCATT
    AATCAGCCTC TGCTCACCTT GGCTTCATCT GCTACAGCAT CTTCAATCCC AGGGGCATCA
    ACTGTAGTTC CTAATCAGCT TCCAACCCTT CTGCAGCCTG TGAATCAGCT GCAGAGTCAG
    GTTCACCCAC AGCTCCTACA GCCAACAACA GTTCAGTCCA TGGGATTACC AGCTAACCTT
    GGACAAGCTG CTGAGTGTCC ACTTCCCCCT GGAGATGTTC TGTACCAGGG CTTCCCATCT
    CGACTGCCAC CACAATACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTAGCCCCT CCTCCTATGC CAACAGAAGC ATTGGCCACC
    CAAGGTTACT TTCCTACAGT AGTGCAGCCT TATGTGGAAT CAACTCCTTT GGTTCCTATG
    GGCAGTGTAG GAGGCCAGGT TCAAGTGTCC CAACCAGCAG TGAGTTTATC GCAACAACCC
    CCCACTACAT CCTCCCAGCA AGCAGTTTTG GAGAGTAGTA CACAGGGAGT CTCTCAGGCT
    GCTCCTCCAG AACAAACTCC AATAACACAG TCACAACCTA CTCAACCTGT TCCATTGGTT
    TCCTCTGTGG ACAGTGCCCA TTCAGATGTT GCTTCTGGTA TGAGTGATGG CAATGAGAAT
    GCTCCTTCAT CCAGCGGGAG GCATGAAGGG AGGACGACAA AACGTCATTA TCGAAAATCT
    GTAAGAAGTC GTTCTCGTCA TGAAAAGACT TCTCGCCCGA AACTGAGGAT TTTAAATGTT
    TCAAATAAAG GAGACCGAGT AGTAGAATGT CAATTGGAGA CTCATAATCG GAAAATGGTT
    ACATTTAAAT TTGACCTAGA TGGTGACAAC CCTGAAGAGA TAGCAACAAT TATGGTGAAC
    AATGACTTTA TTCTAGCCAT AGAGAGAGAG TCTTTTGTGG CTCAAGTGCG GGAAATTATT
    GAAAAGGCTG ATGAAATGTT AAGCGAGGAT GTCAGTGTGG AGCCTGAGGG TGACCAGGGA
    TTGGAGAGTC TACAAGGAAA GGATGACTAT GGCTTTCCAG GTTCTCAGAA ATTAGAAGGA
    GAGTTCAAGC AACCAATTGC TGTATCTTCC ATGCCACAAC AAATAGGTGT TCCTACCAGT
    TCTTTAACAC AAGTTGTTCA TTCTGCTGGA AGACGATTTA TAGTGAGTCC AGTTCCAGAA
    AGTCGATTAC GAGAATCAAA AATTTTCACC AGTGAAATAC CAGATCCAGT TGCGGCCTCT
    ACATCTCAGG GCCCTGGAAT GAACTTATCT CACTCCGCTT CATCCCTTAG TCTCCAGCAG
    GCTTTTTCTG AACTGAAACA TGGTCAGATG ACTGAAGGAC CCAATACAGC ACCTCCAAAT
    TTTAATCACC CAGGACCAAC ATTTTCTCCC TTTTTGACTA GCATTGCTGG TGTCCAAACT
    GTAGCAGCAT CCACACCATC AGTATCAGTC CCTATAACAA GCAGCCCTCT TAATGACATT
    TCCACATCAG TGATGCAGTC AGAGGGTGCA CTGCCCACTG ACAAAGGCAT TGGGGGAGTT
    ACCACCAGCA CAGGTGTGGT AGCTTCAGGT GGTCTCACCA CATTGTCTGT GTCTGAGACA
    CCAACACTTT CCAGTGCAGT TTCAAGTTCT ACAGCGCCTG CAGTTGTCAC AGTGTCTACA
    ACATCCCAAC CAGTTCAGGC TTCCACATCT GGGAGCATTG CTTCTAGTAC AGGCAGTTTT
    CCTTCTGGAA CATTTTCCAC AACTACAGGT ACCACAGTGA GTAGTGTAGC TGTCCCAAAT
    GCTAAGCCTC CAACTGTATT ACTGCAACAG GTAGCAGGTA ATACTGCTGG GGTAGCCATA
    GTGACATCAG TTTCTACTAC CACTCCATTC CCAGCCATGG CTTCACAGCC ATCCCTTCCA
    CTTGGTAGCA GCACATCTGC TCCTACACTA GCTGAAACAG TGGTGGTTAG TGCACACTCA
    CTAGACAAGG CATCTCACAG TAGTACAGCA GGATTGGGTT TGTCCTTTTG CGCACCGTCC
    TCTTCTTCCT CCTCTGGAAC AGCAGTGTCT AGTTCTGTTT CTCAGCCTGG TATTGTGCAT
    CCTTTGGTCA TCTCATCAGC TATAGCTTCT ACACCTGTCC TCCCACAACC AGCAGTACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AATATCCCAC CCTTGGTACA ACCTGTTGCT
    AATGTGCCTG CTGTACAGCA AACACTCATT CATAGTCAGC CTCAACCAGC TCTACTTCCC
    AACCAGCCTC ACACACACTG TCCCGAAATG GATGCTGATA CCCAGTCCAA AGCTCCTGGG
    ATCGATGATA TAAAGACTCT GGAGGAAAAG CTGAGATCGC TCTTTAGTGA GCATAGCTCA
    TCTGGAACTC AGCATGCTTC TGTCTCCCTG GAGACACCCT TAGTAGTAGA GACTGTCACA
    CCGGGCATCC CTACCACTGC TGTAGCACCA AGTAAACTCA TGACTTCCAC TACAAGCACT
    TGCTTGCCAC CAACCAATTT ACCCTTAGGA ACAGCTGGCA TGCCAGTTAT GCCAGTGGGC
    ACACCTGGTC AAGTTTCTAC CCCAGGCACA CATGCCTCAG CCCCAGCCAG CACCGCAACA
    GGAGCAAAAC CTGGAACAAC TCCCCCAAAG CCATCTTTAA CTAAGACTGT GGTGCCGCCA
    GTGGGTACTG AGCTTTCGGC TGGTACTGTA CCCTGTGAGC AGCTGCCACC TTTTCCCGGA
    CCGTCTCTAA TTCAGACCCA GCAACCCCTA GAAGATCTTG ATGCTCAGTT GAGAAGAACA
    CTTAGTCCAG AGACTATTCC AGTGACACCC GCAGTTGGTC CTTTGTCTAC GATGTCTTCA
    ACGGCAGTTA CAGAAGCAGG AAGTCAACCT CAGAAGGATG GCACAGAAGT TCATGTCACA
    GCAAGTAGCT CAGGAGCTGG TGTTGTTAAG ATGGGGAGAT TTCAGGTTTC AGTTACAATG
    GATGATGCCC AGAAAGAGCG TAAAAATAGG TCAGAAGATA CAAAGTCGGT TCATTTTGAA
    TCTAGTACAT CAGAGTCATC AGTTCTTTCA AGTAGTAGTC CAGAGAGTAC CCTGGTAAAG
    CCAGAGCCTA ATGGAATTAC TGTTTCTGGC ATCTCCTTAG ATGTGCCAGA CAGTACCCAT
    AGAACACCGA CCCCAGAAGC AAAGTCAGAA ACAGGACAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCCAA CAAAGTGGGT CGTTTCTCTG TTTCAAGAAC TGAGGACAAG
    GTCACAGAGC TCAAAAAAGA GGGACCAGTG ACCTCTCCTT TCAGAGATTC AGAACAAACT
    GTTATTCCTG CTGCGATACC AAAGAAGGAG AAGCCTGAGC TGGCAGAACC TTCCCATCTG
    AATGGGCCAT CTTCAGACTT GGAGGCTGCC TTTCTAAGTA GGGGTGGAGA GGATGGATCA
    GGTAGTCCTC ACTCTCCCCC TCACCTGTGT TCAAAGAGCC TTCCCATCCA GACTCTAAGT
    CAAAGCCTTA GTAATTCATT CAACTCCTCT TACATGAGTA GTGACAATGA GTCCGACATT
    GAAGATGAAG ACTTAAGGTT AGAACTGCGA CGACTACGGG AAAAACATCT TAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTTCCCCCTG CTGTCATTAT TCCCCCAGCT GCTCCTCTGT CAGGGAGAAG AAGGAGGCCT
    ACTAAAAGCA AAGGCAGCAA GTCCAGTCGC AGCAGCTCAT TGGGCAACAA AAGCCCTCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGGA ACTTCAGTCT TAAACCCCCA ACAGACCCTC
    CATCCCCCAG GCAATACCCC AGAGACTGGA CACAACCAGC TGTTACAGCC TCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTTACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGGACCAGC AGCACAAACA CCGTTGGAGG AACAGTGAGT
    AGCCAGGCTG CCCAGGCTCA GCCTCCTGCC ATGACTTCCA GTAGGAAGGG CACATTCACA
    GATGACCTGC ACAAGTTGGT AGACAACTGG GCCCGAGATG CCATGAATCT TTCAGGCAGG
    AGAGGCAGCA AAGGACACAT GAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCT
    CCTGGGCAGC TGTGCATTTC CATGACCTCA AACATGGGTG GCTCTACCCC CATCTCTGCA
    GCATCTGCTA CCTCTCTAGG TCACTTCACC AAGTCCATGT GCCCCCCACA GCAGTATGGT
    TTTCCAGCTG CCCCATTCGG CACTCAGTGG AGTGGAACAG GTGGTCCAGC ACCACAGCCA
    CTTGGTCAGT TCCAACCTGT AGGAACCACA TCCTTGCAAA ATTTCAACAT CAGCAATTTG
    CAGAAGTCCA TCAGCAACCC CCCAGGTTCC AACCTACGGA CCACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43985
    Clone ID Related Accession (Same CDS sequence) XM_008763211.2
    Accession Version XM_008763211.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763211.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGGGCT TCCCATCTCG ACTGCCACCA CAATACCCAG GAGATTCAAA TATTGCTCCC
    TCTTCCAACG TGGCTTCTGT TTGCATCCAT TCTACAGTCC TAGCCCCTCC TCCTATGCCA
    ACAGAAGCAT TGGCCACCCA AGGTTACTTT CCTACAGTAG TGCAGCCTTA TGTGGAATCA
    ACTCCTTTGG TTCCTATGGG CAGTGTAGGA GGCCAGGTTC AAGTGTCCCA ACCAGCAGTG
    AGTTTATCGC AACAACCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTAGTACA
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAACCTGTTC CATTGGTTTC CTCTGTGGAC AGTGCCCATT CAGATGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCTTCATCC AGCGGGAGGC ATGAAGGGAG GACGACAAAA
    CGTCATTATC GAAAATCTGT AAGAAGTCGT TCTCGTCATG AAAAGACTTC TCGCCCGAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTGGAGACT
    CATAATCGGA AAATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCCATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GCGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTA CAAGGAAAGG ATGACTATGG CTTTCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACACAA GTTGTTCATT CTGCTGGAAG ACGATTTATA
    GTGAGTCCAG TTCCAGAAAG TCGATTACGA GAATCAAAAA TTTTCACCAG TGAAATACCA
    GATCCAGTTG CGGCCTCTAC ATCTCAGGGC CCTGGAATGA ACTTATCTCA CTCCGCTTCA
    TCCCTTAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACCCA GGACCAACAT TTTCTCCCTT TTTGACTAGC
    ATTGCTGGTG TCCAAACTGT AGCAGCATCC ACACCATCAG TATCAGTCCC TATAACAAGC
    AGCCCTCTTA ATGACATTTC CACATCAGTG ATGCAGTCAG AGGGTGCACT GCCCACTGAC
    AAAGGCATTG GGGGAGTTAC CACCAGCACA GGTGTGGTAG CTTCAGGTGG TCTCACCACA
    TTGTCTGTGT CTGAGACACC AACACTTTCC AGTGCAGTTT CAAGTTCTAC AGCGCCTGCA
    GTTGTCACAG TGTCTACAAC ATCCCAACCA GTTCAGGCTT CCACATCTGG GAGCATTGCT
    TCTAGTACAG GCAGTTTTCC TTCTGGAACA TTTTCCACAA CTACAGGTAC CACAGTGAGT
    AGTGTAGCTG TCCCAAATGC TAAGCCTCCA ACTGTATTAC TGCAACAGGT AGCAGGTAAT
    ACTGCTGGGG TAGCCATAGT GACATCAGTT TCTACTACCA CTCCATTCCC AGCCATGGCT
    TCACAGCCAT CCCTTCCACT TGGTAGCAGC ACATCTGCTC CTACACTAGC TGAAACAGTG
    GTGGTTAGTG CACACTCACT AGACAAGGCA TCTCACAGTA GTACAGCAGG ATTGGGTTTG
    TCCTTTTGCG CACCGTCCTC TTCTTCCTCC TCTGGAACAG CAGTGTCTAG TTCTGTTTCT
    CAGCCTGGTA TTGTGCATCC TTTGGTCATC TCATCAGCTA TAGCTTCTAC ACCTGTCCTC
    CCACAACCAG CAGTACCTAC TTCTACACCT TTATTACCCC AAGTACCTAA TATCCCACCC
    TTGGTACAAC CTGTTGCTAA TGTGCCTGCT GTACAGCAAA CACTCATTCA TAGTCAGCCT
    CAACCAGCTC TACTTCCCAA CCAGCCTCAC ACACACTGTC CCGAAATGGA TGCTGATACC
    CAGTCCAAAG CTCCTGGGAT CGATGATATA AAGACTCTGG AGGAAAAGCT GAGATCGCTC
    TTTAGTGAGC ATAGCTCATC TGGAACTCAG CATGCTTCTG TCTCCCTGGA GACACCCTTA
    GTAGTAGAGA CTGTCACACC GGGCATCCCT ACCACTGCTG TAGCACCAAG TAAACTCATG
    ACTTCCACTA CAAGCACTTG CTTGCCACCA ACCAATTTAC CCTTAGGAAC AGCTGGCATG
    CCAGTTATGC CAGTGGGCAC ACCTGGTCAA GTTTCTACCC CAGGCACACA TGCCTCAGCC
    CCAGCCAGCA CCGCAACAGG AGCAAAACCT GGAACAACTC CCCCAAAGCC ATCTTTAACT
    AAGACTGTGG TGCCGCCAGT GGGTACTGAG CTTTCGGCTG GTACTGTACC CTGTGAGCAG
    CTGCCACCTT TTCCCGGACC GTCTCTAATT CAGACCCAGC AACCCCTAGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTCCAG TGACACCCGC AGTTGGTCCT
    TTGTCTACGA TGTCTTCAAC GGCAGTTACA GAAGCAGGAA GTCAACCTCA GAAGGATGGC
    ACAGAAGTTC ATGTCACAGC AAGTAGCTCA GGAGCTGGTG TTGTTAAGAT GGGGAGATTT
    CAGGTTTCAG TTACAATGGA TGATGCCCAG AAAGAGCGTA AAAATAGGTC AGAAGATACA
    AAGTCGGTTC ATTTTGAATC TAGTACATCA GAGTCATCAG TTCTTTCAAG TAGTAGTCCA
    GAGAGTACCC TGGTAAAGCC AGAGCCTAAT GGAATTACTG TTTCTGGCAT CTCCTTAGAT
    GTGCCAGACA GTACCCATAG AACACCGACC CCAGAAGCAA AGTCAGAAAC AGGACAGCCT
    ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCCAACA AAGTGGGTCG TTTCTCTGTT
    TCAAGAACTG AGGACAAGGT CACAGAGCTC AAAAAAGAGG GACCAGTGAC CTCTCCTTTC
    AGAGATTCAG AACAAACTGT TATTCCTGCT GCGATACCAA AGAAGGAGAA GCCTGAGCTG
    GCAGAACCTT CCCATCTGAA TGGGCCATCT TCAGACTTGG AGGCTGCCTT TCTAAGTAGG
    GGTGGAGAGG ATGGATCAGG TAGTCCTCAC TCTCCCCCTC ACCTGTGTTC AAAGAGCCTT
    CCCATCCAGA CTCTAAGTCA AAGCCTTAGT AATTCATTCA ACTCCTCTTA CATGAGTAGT
    GACAATGAGT CCGACATTGA AGATGAAGAC TTAAGGTTAG AACTGCGACG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG
    TATACCAAAC TGGGCAAGGT TCCCCCTGCT GTCATTATTC CCCCAGCTGC TCCTCTGTCA
    GGGAGAAGAA GGAGGCCTAC TAAAAGCAAA GGCAGCAAGT CCAGTCGCAG CAGCTCATTG
    GGCAACAAAA GCCCTCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGGAAC TTCAGTCTTA
    AACCCCCAAC AGACCCTCCA TCCCCCAGGC AATACCCCAG AGACTGGACA CAACCAGCTG
    TTACAGCCTC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT TACCAGTGAT
    GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GGACCAGCAG CACAAACACC
    GTTGGAGGAA CAGTGAGTAG CCAGGCTGCC CAGGCTCAGC CTCCTGCCAT GACTTCCAGT
    AGGAAGGGCA CATTCACAGA TGACCTGCAC AAGTTGGTAG ACAACTGGGC CCGAGATGCC
    ATGAATCTTT CAGGCAGGAG AGGCAGCAAA GGACACATGA ATTATGAGGG CCCTGGAATG
    GCAAGGAAGT TCTCTGCTCC TGGGCAGCTG TGCATTTCCA TGACCTCAAA CATGGGTGGC
    TCTACCCCCA TCTCTGCAGC ATCTGCTACC TCTCTAGGTC ACTTCACCAA GTCCATGTGC
    CCCCCACAGC AGTATGGTTT TCCAGCTGCC CCATTCGGCA CTCAGTGGAG TGGAACAGGT
    GGTCCAGCAC CACAGCCACT TGGTCAGTTC CAACCTGTAG GAACCACATC CTTGCAAAAT
    TTCAACATCA GCAATTTGCA GAAGTCCATC AGCAACCCCC CAGGTTCCAA CCTACGGACC
    ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008761433.1
    CDS232..6897
    Translation

    Target ORF information:

    RefSeq Version XM_008763211.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008763211.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGGGCT TCCCATCTCG ACTGCCACCA CAATACCCAG GAGATTCAAA TATTGCTCCC
    TCTTCCAACG TGGCTTCTGT TTGCATCCAT TCTACAGTCC TAGCCCCTCC TCCTATGCCA
    ACAGAAGCAT TGGCCACCCA AGGTTACTTT CCTACAGTAG TGCAGCCTTA TGTGGAATCA
    ACTCCTTTGG TTCCTATGGG CAGTGTAGGA GGCCAGGTTC AAGTGTCCCA ACCAGCAGTG
    AGTTTATCGC AACAACCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTAGTACA
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAACCTGTTC CATTGGTTTC CTCTGTGGAC AGTGCCCATT CAGATGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCTTCATCC AGCGGGAGGC ATGAAGGGAG GACGACAAAA
    CGTCATTATC GAAAATCTGT AAGAAGTCGT TCTCGTCATG AAAAGACTTC TCGCCCGAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTGGAGACT
    CATAATCGGA AAATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCCATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GCGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTA CAAGGAAAGG ATGACTATGG CTTTCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACACAA GTTGTTCATT CTGCTGGAAG ACGATTTATA
    GTGAGTCCAG TTCCAGAAAG TCGATTACGA GAATCAAAAA TTTTCACCAG TGAAATACCA
    GATCCAGTTG CGGCCTCTAC ATCTCAGGGC CCTGGAATGA ACTTATCTCA CTCCGCTTCA
    TCCCTTAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACCCA GGACCAACAT TTTCTCCCTT TTTGACTAGC
    ATTGCTGGTG TCCAAACTGT AGCAGCATCC ACACCATCAG TATCAGTCCC TATAACAAGC
    AGCCCTCTTA ATGACATTTC CACATCAGTG ATGCAGTCAG AGGGTGCACT GCCCACTGAC
    AAAGGCATTG GGGGAGTTAC CACCAGCACA GGTGTGGTAG CTTCAGGTGG TCTCACCACA
    TTGTCTGTGT CTGAGACACC AACACTTTCC AGTGCAGTTT CAAGTTCTAC AGCGCCTGCA
    GTTGTCACAG TGTCTACAAC ATCCCAACCA GTTCAGGCTT CCACATCTGG GAGCATTGCT
    TCTAGTACAG GCAGTTTTCC TTCTGGAACA TTTTCCACAA CTACAGGTAC CACAGTGAGT
    AGTGTAGCTG TCCCAAATGC TAAGCCTCCA ACTGTATTAC TGCAACAGGT AGCAGGTAAT
    ACTGCTGGGG TAGCCATAGT GACATCAGTT TCTACTACCA CTCCATTCCC AGCCATGGCT
    TCACAGCCAT CCCTTCCACT TGGTAGCAGC ACATCTGCTC CTACACTAGC TGAAACAGTG
    GTGGTTAGTG CACACTCACT AGACAAGGCA TCTCACAGTA GTACAGCAGG ATTGGGTTTG
    TCCTTTTGCG CACCGTCCTC TTCTTCCTCC TCTGGAACAG CAGTGTCTAG TTCTGTTTCT
    CAGCCTGGTA TTGTGCATCC TTTGGTCATC TCATCAGCTA TAGCTTCTAC ACCTGTCCTC
    CCACAACCAG CAGTACCTAC TTCTACACCT TTATTACCCC AAGTACCTAA TATCCCACCC
    TTGGTACAAC CTGTTGCTAA TGTGCCTGCT GTACAGCAAA CACTCATTCA TAGTCAGCCT
    CAACCAGCTC TACTTCCCAA CCAGCCTCAC ACACACTGTC CCGAAATGGA TGCTGATACC
    CAGTCCAAAG CTCCTGGGAT CGATGATATA AAGACTCTGG AGGAAAAGCT GAGATCGCTC
    TTTAGTGAGC ATAGCTCATC TGGAACTCAG CATGCTTCTG TCTCCCTGGA GACACCCTTA
    GTAGTAGAGA CTGTCACACC GGGCATCCCT ACCACTGCTG TAGCACCAAG TAAACTCATG
    ACTTCCACTA CAAGCACTTG CTTGCCACCA ACCAATTTAC CCTTAGGAAC AGCTGGCATG
    CCAGTTATGC CAGTGGGCAC ACCTGGTCAA GTTTCTACCC CAGGCACACA TGCCTCAGCC
    CCAGCCAGCA CCGCAACAGG AGCAAAACCT GGAACAACTC CCCCAAAGCC ATCTTTAACT
    AAGACTGTGG TGCCGCCAGT GGGTACTGAG CTTTCGGCTG GTACTGTACC CTGTGAGCAG
    CTGCCACCTT TTCCCGGACC GTCTCTAATT CAGACCCAGC AACCCCTAGA AGATCTTGAT
    GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTCCAG TGACACCCGC AGTTGGTCCT
    TTGTCTACGA TGTCTTCAAC GGCAGTTACA GAAGCAGGAA GTCAACCTCA GAAGGATGGC
    ACAGAAGTTC ATGTCACAGC AAGTAGCTCA GGAGCTGGTG TTGTTAAGAT GGGGAGATTT
    CAGGTTTCAG TTACAATGGA TGATGCCCAG AAAGAGCGTA AAAATAGGTC AGAAGATACA
    AAGTCGGTTC ATTTTGAATC TAGTACATCA GAGTCATCAG TTCTTTCAAG TAGTAGTCCA
    GAGAGTACCC TGGTAAAGCC AGAGCCTAAT GGAATTACTG TTTCTGGCAT CTCCTTAGAT
    GTGCCAGACA GTACCCATAG AACACCGACC CCAGAAGCAA AGTCAGAAAC AGGACAGCCT
    ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCCAACA AAGTGGGTCG TTTCTCTGTT
    TCAAGAACTG AGGACAAGGT CACAGAGCTC AAAAAAGAGG GACCAGTGAC CTCTCCTTTC
    AGAGATTCAG AACAAACTGT TATTCCTGCT GCGATACCAA AGAAGGAGAA GCCTGAGCTG
    GCAGAACCTT CCCATCTGAA TGGGCCATCT TCAGACTTGG AGGCTGCCTT TCTAAGTAGG
    GGTGGAGAGG ATGGATCAGG TAGTCCTCAC TCTCCCCCTC ACCTGTGTTC AAAGAGCCTT
    CCCATCCAGA CTCTAAGTCA AAGCCTTAGT AATTCATTCA ACTCCTCTTA CATGAGTAGT
    GACAATGAGT CCGACATTGA AGATGAAGAC TTAAGGTTAG AACTGCGACG ACTACGGGAA
    AAACATCTTA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG
    TATACCAAAC TGGGCAAGGT TCCCCCTGCT GTCATTATTC CCCCAGCTGC TCCTCTGTCA
    GGGAGAAGAA GGAGGCCTAC TAAAAGCAAA GGCAGCAAGT CCAGTCGCAG CAGCTCATTG
    GGCAACAAAA GCCCTCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGGAAC TTCAGTCTTA
    AACCCCCAAC AGACCCTCCA TCCCCCAGGC AATACCCCAG AGACTGGACA CAACCAGCTG
    TTACAGCCTC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT TACCAGTGAT
    GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GGACCAGCAG CACAAACACC
    GTTGGAGGAA CAGTGAGTAG CCAGGCTGCC CAGGCTCAGC CTCCTGCCAT GACTTCCAGT
    AGGAAGGGCA CATTCACAGA TGACCTGCAC AAGTTGGTAG ACAACTGGGC CCGAGATGCC
    ATGAATCTTT CAGGCAGGAG AGGCAGCAAA GGACACATGA ATTATGAGGG CCCTGGAATG
    GCAAGGAAGT TCTCTGCTCC TGGGCAGCTG TGCATTTCCA TGACCTCAAA CATGGGTGGC
    TCTACCCCCA TCTCTGCAGC ATCTGCTACC TCTCTAGGTC ACTTCACCAA GTCCATGTGC
    CCCCCACAGC AGTATGGTTT TCCAGCTGCC CCATTCGGCA CTCAGTGGAG TGGAACAGGT
    GGTCCAGCAC CACAGCCACT TGGTCAGTTC CAACCTGTAG GAACCACATC CTTGCAAAAT
    TTCAACATCA GCAATTTGCA GAAGTCCATC AGCAACCCCC CAGGTTCCAA CCTACGGACC
    ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43986
    Clone ID Related Accession (Same CDS sequence) XM_008763212.2
    Accession Version XM_008763212.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product serine/threonine-protein kinase WNK1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763212.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGGGCT TCCCATCTCG ACTGCCACCA CAATACCCAG GAGATTCAAA TATTGCTCCC
    TCTTCCAACG TGGCTTCTGT TTGCATCCAT TCTACAGTCC TAGCCCCTCC TCCTATGCCA
    ACAGAAGCAT TGGCCACCCA AGGTTACTTT CCTACAGTAG TGCAGCCTTA TGTGGAATCA
    ACTCCTTTGG TTCCTATGGG CAGTGTAGGA GGCCAGGTTC AAGTGTCCCA ACCAGCAGTG
    AGTTTATCGC AACAACCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTAGTACA
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAACCTGTTC CATTGGTTTC CTCTGTGGAC AGTGCCCATT CAGATGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCTTCATCC AGCGGGAGGC ATGAAGGGAG GACGACAAAA
    CGTCATTATC GAAAATCTGT AAGAAGTCGT TCTCGTCATG AAAAGACTTC TCGCCCGAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTGGAGACT
    CATAATCGGA AAATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCCATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GCGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTA CAAGGAAAGG ATGACTATGG CTTTCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACACAA GTTGTTCATT CTGCTGGAAG ACGATTTATA
    GTGAGTCCAG TTCCAGAAAG TCGATTACGA GAATCAAAAA TTTTCACCAG TGAAATACCA
    GATCCAGTTG CGGCCTCTAC ATCTCAGGGC CCTGGAATGA ACTTATCTCA CTCCGCTTCA
    TCCCTTAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACCCA GGACCAACAT TTTCTCCCTT TTTGACTAGC
    ATTGCTGGTG TCCAAACTGT AGCAGCATCC ACACCATCAG TATCAGTCCC TATAACAAGC
    AGCCCTCTTA ATGACATTTC CACATCAGTG ATGCAGTCAG AGGGTGCACT GCCCACTGAC
    AAAGGCATTG GGGGAGTTAC CACCAGCACA GGTGTGGTAG CTTCAGGTGG TCTCACCACA
    TTGTCTGTGT CTGAGACACC AACACTTTCC AGTGCAGTTT CAAGTTCTAC AGCGCCTGCA
    GTTGTCACAG TGTCTACAAC ATCCCAACCA GTTCAGGCTT CCACATCTGG GAGCATTGCT
    TCTAGTACAG GCAGTTTTCC TTCTGGAACA TTTTCCACAA CTACAGGTAC CACAGTGAGT
    AGTGTAGCTG TCCCAAATGC TAAGCCTCCA ACTGTATTAC TGCAACAGGT AGCAGGTAAT
    ACTGCTGGGG TAGCCATAGT GACATCAGTT TCTACTACCA CTCCATTCCC AGCCATGGCT
    TCACAGCCAT CCCTTCCACT TGGTAGCAGC ACATCTGCTC CTACACTAGC TGAAACAGTG
    GTGGTTAGTG CACACTCACT AGACAAGGCA TCTCACAGTA GTACAGCAGG ATTGGGTTTG
    TCCTTTTGCG CACCGTCCTC TTCTTCCTCC TCTGGAACAG CAGTGTCTAG TTCTGTTTCT
    CAGCCTGGTA TTGTGCATCC TTTGGTCATC TCATCAGCTA TAGCTTCTAC ACCTGTCCTC
    CCACAACCAG CAGTACCTAC TTCTACACCT TTATTACCCC AAGTACCTAA TATCCCACCC
    TTGGTACAAC CTGTTGCTAA TGTGCCTGCT GTACAGCAAA CACTCATTCA TAGTCAGCCT
    CAACCAGCTC TACTTCCCAA CCAGCCTCAC ACACACTGTC CCGAAATGGA TGCTGATACC
    CAGTCCAAAG CTCCTGGGAT CGATGATATA AAGACTCTGG AGGAAAAGCT GAGATCGCTC
    TTTAGTGAGC ATAGCTCATC TGGAACTCAG CATGCTTCTG TCTCCCTGGA GACACCCTTA
    GTAGTAGAGA CTGTCACACC GGGCATCCCT ACCACTGCTG TAGCACCAAG TAAACTCATG
    ACTTCCACTA CAAGCACTTG CTTGCCACCA ACCAATTTAC CCTTAGGAAC AGCTGGCATG
    CCAGTTATGC CAGTGGGCAC ACCTGGTCAA GTTTCTACCC CAGGCACACA TGCCTCAGCC
    CCAGCCAGCA CCGCAACAGG AGCAAAACCT GGAACAACTC CCCCAAAGCC ATCTTTAACT
    AAGACTGTGG TGCCGCCAGT GGGTACTGAG CTTTCGGCTG GTACTGTACC CTGTGAGCAG
    CTGCCACCTT TTCCCGGACC GTCTCTAATT CAGCCTTTGT CTACGATGTC TTCAACGGCA
    GTTACAGAAG CAGGAAGTCA ACCTCAGAAG GATGGCACAG AAGTTCATGT CACAGCAAGT
    AGCTCAGGAG CTGGTGTTGT TAAGATGGGG AGATTTCAGG TTTCAGTTAC AATGGATGAT
    GCCCAGAAAG AGCGTAAAAA TAGGTCAGAA GATACAAAGT CGGTTCATTT TGAATCTAGT
    ACATCAGAGT CATCAGTTCT TTCAAGTAGT AGTCCAGAGA GTACCCTGGT AAAGCCAGAG
    CCTAATGGAA TTACTGTTTC TGGCATCTCC TTAGATGTGC CAGACAGTAC CCATAGAACA
    CCGACCCCAG AAGCAAAGTC AGAAACAGGA CAGCCTACCA AGGTTGGACG TTTTCAGGTG
    ACAACTACAG CCAACAAAGT GGGTCGTTTC TCTGTTTCAA GAACTGAGGA CAAGGTCACA
    GAGCTCAAAA AAGAGGGACC AGTGACCTCT CCTTTCAGAG ATTCAGAACA AACTGTTATT
    CCTGCTGCGA TACCAAAGAA GGAGAAGCCT GAGCTGGCAG AACCTTCCCA TCTGAATGGG
    CCATCTTCAG ACTTGGAGGC TGCCTTTCTA AGTAGGGGTG GAGAGGATGG ATCAGGTAGT
    CCTCACTCTC CCCCTCACCT GTGTTCAAAG AGCCTTCCCA TCCAGACTCT AAGTCAAAGC
    CTTAGTAATT CATTCAACTC CTCTTACATG AGTAGTGACA ATGAGTCCGA CATTGAAGAT
    GAAGACTTAA GGTTAGAACT GCGACGACTA CGGGAAAAAC ATCTTAAAGA GATTCAGGAC
    CTGCAGAGTC GCCAGAAGCA TGAAATTGAA TCTTTGTATA CCAAACTGGG CAAGGTTCCC
    CCTGCTGTCA TTATTCCCCC AGCTGCTCCT CTGTCAGGGA GAAGAAGGAG GCCTACTAAA
    AGCAAAGGCA GCAAGTCCAG TCGCAGCAGC TCATTGGGCA ACAAAAGCCC TCAGCTTTCA
    GGTAACCTGT CTGGTCAGAG TGGAACTTCA GTCTTAAACC CCCAACAGAC CCTCCATCCC
    CCAGGCAATA CCCCAGAGAC TGGACACAAC CAGCTGTTAC AGCCTCTTAA GCCATCTCCC
    TCCAGTGACA ACCTCTATTC AGCCTTTACC AGTGATGGTG CCATTTCAGT ACCAAGCCTT
    TCTGCTCCAG GTCAAGGGAC CAGCAGCACA AACACCGTTG GAGGAACAGT GAGTAGCCAG
    GCTGCCCAGG CTCAGCCTCC TGCCATGACT TCCAGTAGGA AGGGCACATT CACAGATGAC
    CTGCACAAGT TGGTAGACAA CTGGGCCCGA GATGCCATGA ATCTTTCAGG CAGGAGAGGC
    AGCAAAGGAC ACATGAATTA TGAGGGCCCT GGAATGGCAA GGAAGTTCTC TGCTCCTGGG
    CAGCTGTGCA TTTCCATGAC CTCAAACATG GGTGGCTCTA CCCCCATCTC TGCAGCATCT
    GCTACCTCTC TAGGTCACTT CACCAAGTCC ATGTGCCCCC CACAGCAGTA TGGTTTTCCA
    GCTGCCCCAT TCGGCACTCA GTGGAGTGGA ACAGGTGGTC CAGCACCACA GCCACTTGGT
    CAGTTCCAAC CTGTAGGAAC CACATCCTTG CAAAATTTCA ACATCAGCAA TTTGCAGAAG
    TCCATCAGCA ACCCCCCAGG TTCCAACCTA CGGACCACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008761434.1
    CDS232..6813
    Translation

    Target ORF information:

    RefSeq Version XM_008763212.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus WNK lysine deficient protein kinase 1 (Wnk1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008763212.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGTCTGACG GCACCGCAGA GAAGCAGAGC GGCACTCCTG GCTTCCTTTC GCCTCCGGCT 
    CCAGTCCCCA AGAATGGCTC AAGCTCCGAT TCTTCCGTGG GCGAGAAACT GGGGGCCGCG
    GTCGCCGACT CCGGAATTGG CAGGACAGAG GAATACCGGC GCCGCCGGCA CACTATGGAC
    AAGGACAGTC GTGGGGCGGC CGCCACCACT ACCCCCACGG AGCACCGATT TTTCCGCCGA
    AGCGTCATCT GCGACTCCAA TGCCACGGCG TTGGAGCTGC CGGGCCTCCC TCTCTCTATC
    CCTCAACCTA GTGTACCCGC AGTGGTGCCT CAGAGTGCTC CACCCGAGCC TCACAGAGAG
    GAAACTTTGA CGGCCACCGT TGCTTCCCAG GTGTCTCAGC AACCCTCGGC TGCTGCCTCC
    CCTGGGGAAC AAGCCGTTGT AGGCTCGGCC ACCGCGACCG TCCCCAGCAG TACCAGCAAA
    GACCGCCCGG TGTCCCAGCC CAGCCTCGTG GGTAGCAAAG AGGAGCCGCC GCCGTCCAGA
    AGTGGAAGTG GCAGCGGTGG TGCCAGTGCC AAAGAGCCGC AGGAGGAAAG GAACCAGCAA
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCAGTGGGAA TGTCCAATGA CGGTCGCTTT
    CTCAAATTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACCGAAACCA CTGTGGAAGT CGCCTGGTGT GAATTGCAGG ATCGAAAGTT AACAAAATCT
    GAAAGGCAGA GATTCAAAGA AGAAGCTGAA ATGCTAAAGG GTCTTCAACA TCCTAACATT
    GTTCGATTCT ATGATTCCTG GGAATCCACA GTAAAAGGGA AAAAATGCAT TGTTTTAGTG
    ACTGAACTAA TGACATCTGG AACACTTAAA ACGTACTTAA AAAGGTTTAA AGTGATGAAA
    ATCAAAGTTT TAAGAAGCTG GTGTCGGCAA ATCCTTAAAG GACTTCAATT TCTCCATACA
    CGAACTCCCC CGATTATTCA CCGGGATCTT AAATGTGACA ACATCTTTAT CACTGGTCCT
    ACCGGCTCAG TCAAGATCGG AGACCTTGGT CTGGCAACTC TAAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTTATG GCTCCTGAGA TGTATGAGGA GAAGTACGAT
    GAATCCGTTG ATGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC GTCTGAATAT
    CCATACTCAG AGTGCCAAAA TGCTGCTCAG ATCTACCGTC GAGTGACCAG TGGAGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGTATC
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTTTTCCAG
    GAGGAAACAG GGGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA GATAGCTATC
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGACAATGAA
    GCTATTGAGT TTTCCTTTGA CTTGGAGAGG GATGTACCAG AAGATGTTGC TCAAGAAATG
    GTCGAGTCTG GTTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAT
    AGGGTATCAT TAATTAAGAG AAAACGAGAG CAGCGGCAGT TGGTTCGAGA AGAACAAGAA
    AAAAGAAAAC AAGAAGAGAG CAGTTTCAAA CAGCAGAATG AACAACAAGC AAGTGTTTCC
    CAGGCAGGAA TCCAGCCACT CTCTGTTGCT AGCACTGGCA TACCCACTGC TCCCACCACA
    TCCGCTTCAG TTTCTACTCA AGTAGAGCCT GAAGAACCCG AGGCAGATCA GCATCAACAG
    CTGCAGTATC AGCAACCTAG TATATCTGTG TTGTCTGATG GAACAGTTGA CAGTGGTCAA
    GGATCATCTG TATTCACAGA ATCCAGAGTG AGCAGCCAAC AGACTGTTTC CTATGGCTCC
    CAACATGAGC AGGCACATTC TATTGGCACA GCACCAGGAC ATACAGTTTC TAGTATCCAA
    GCACAATCTC AGCCCCATGG GGTGTATCCA CCCTCAAGTA TGGCACAGGG GCAGAACCAG
    GGCCAGCCAT CAAGTAGTTT AGCAGGGGTT CTATCTTCAC AACCCGTCCA ACATCCTCAG
    CAGCAGGGAA TACAGCCGAC TGTACCTCCT CAACAGGCAG TACAGTATTC ACTTCCACAG
    GCAGCGTCTT CCAGTGAAGG CACTGTCCAG CCAGTGAGTC AGCCTCAAGT GTCAGCTGGA
    ACACAGGGCT TCCCATCTCG ACTGCCACCA CAATACCCAG GAGATTCAAA TATTGCTCCC
    TCTTCCAACG TGGCTTCTGT TTGCATCCAT TCTACAGTCC TAGCCCCTCC TCCTATGCCA
    ACAGAAGCAT TGGCCACCCA AGGTTACTTT CCTACAGTAG TGCAGCCTTA TGTGGAATCA
    ACTCCTTTGG TTCCTATGGG CAGTGTAGGA GGCCAGGTTC AAGTGTCCCA ACCAGCAGTG
    AGTTTATCGC AACAACCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTAGTACA
    CAGGGAGTCT CTCAGGCTGC TCCTCCAGAA CAAACTCCAA TAACACAGTC ACAACCTACT
    CAACCTGTTC CATTGGTTTC CTCTGTGGAC AGTGCCCATT CAGATGTTGC TTCTGGTATG
    AGTGATGGCA ATGAGAATGC TCCTTCATCC AGCGGGAGGC ATGAAGGGAG GACGACAAAA
    CGTCATTATC GAAAATCTGT AAGAAGTCGT TCTCGTCATG AAAAGACTTC TCGCCCGAAA
    CTGAGGATTT TAAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ATTGGAGACT
    CATAATCGGA AAATGGTTAC ATTTAAATTT GACCTAGATG GTGACAACCC TGAAGAGATA
    GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCCATAG AGAGAGAGTC TTTTGTGGCT
    CAAGTGCGGG AAATTATTGA AAAGGCTGAT GAAATGTTAA GCGAGGATGT CAGTGTGGAG
    CCTGAGGGTG ACCAGGGATT GGAGAGTCTA CAAGGAAAGG ATGACTATGG CTTTCCAGGT
    TCTCAGAAAT TAGAAGGAGA GTTCAAGCAA CCAATTGCTG TATCTTCCAT GCCACAACAA
    ATAGGTGTTC CTACCAGTTC TTTAACACAA GTTGTTCATT CTGCTGGAAG ACGATTTATA
    GTGAGTCCAG TTCCAGAAAG TCGATTACGA GAATCAAAAA TTTTCACCAG TGAAATACCA
    GATCCAGTTG CGGCCTCTAC ATCTCAGGGC CCTGGAATGA ACTTATCTCA CTCCGCTTCA
    TCCCTTAGTC TCCAGCAGGC TTTTTCTGAA CTGAAACATG GTCAGATGAC TGAAGGACCC
    AATACAGCAC CTCCAAATTT TAATCACCCA GGACCAACAT TTTCTCCCTT TTTGACTAGC
    ATTGCTGGTG TCCAAACTGT AGCAGCATCC ACACCATCAG TATCAGTCCC TATAACAAGC
    AGCCCTCTTA ATGACATTTC CACATCAGTG ATGCAGTCAG AGGGTGCACT GCCCACTGAC
    AAAGGCATTG GGGGAGTTAC CACCAGCACA GGTGTGGTAG CTTCAGGTGG TCTCACCACA
    TTGTCTGTGT CTGAGACACC AACACTTTCC AGTGCAGTTT CAAGTTCTAC AGCGCCTGCA
    GTTGTCACAG TGTCTACAAC ATCCCAACCA GTTCAGGCTT CCACATCTGG GAGCATTGCT
    TCTAGTACAG GCAGTTTTCC TTCTGGAACA TTTTCCACAA CTACAGGTAC CACAGTGAGT
    AGTGTAGCTG TCCCAAATGC TAAGCCTCCA ACTGTATTAC TGCAACAGGT AGCAGGTAAT
    ACTGCTGGGG TAGCCATAGT GACATCAGTT TCTACTACCA CTCCATTCCC AGCCATGGCT
    TCACAGCCAT CCCTTCCACT TGGTAGCAGC ACATCTGCTC CTACACTAGC TGAAACAGTG
    GTGGTTAGTG CACACTCACT AGACAAGGCA TCTCACAGTA GTACAGCAGG ATTGGGTTTG
    TCCTTTTGCG CACCGTCCTC TTCTTCCTCC TCTGGAACAG CAGTGTCTAG TTCTGTTTCT
    CAGCCTGGTA TTGTGCATCC TTTGGTCATC TCATCAGCTA TAGCTTCTAC ACCTGTCCTC
    CCACAACCAG CAGTACCTAC TTCTACACCT TTATTACCCC AAGTACCTAA TATCCCACCC
    TTGGTACAAC CTGTTGCTAA TGTGCCTGCT GTACAGCAAA CACTCATTCA TAGTCAGCCT
    CAACCAGCTC TACTTCCCAA CCAGCCTCAC ACACACTGTC CCGAAATGGA TGCTGATACC
    CAGTCCAAAG CTCCTGGGAT CGATGATATA AAGACTCTGG AGGAAAAGCT GAGATCGCTC
    TTTAGTGAGC ATAGCTCATC TGGAACTCAG CATGCTTCTG TCTCCCTGGA GACACCCTTA
    GTAGTAGAGA CTGTCACACC GGGCATCCCT ACCACTGCTG TAGCACCAAG TAAACTCATG
    ACTTCCACTA CAAGCACTTG CTTGCCACCA ACCAATTTAC CCTTAGGAAC AGCTGGCATG
    CCAGTTATGC CAGTGGGCAC ACCTGGTCAA GTTTCTACCC CAGGCACACA TGCCTCAGCC
    CCAGCCAGCA CCGCAACAGG AGCAAAACCT GGAACAACTC CCCCAAAGCC ATCTTTAACT
    AAGACTGTGG TGCCGCCAGT GGGTACTGAG CTTTCGGCTG GTACTGTACC CTGTGAGCAG
    CTGCCACCTT TTCCCGGACC GTCTCTAATT CAGCCTTTGT CTACGATGTC TTCAACGGCA
    GTTACAGAAG CAGGAAGTCA ACCTCAGAAG GATGGCACAG AAGTTCATGT CACAGCAAGT
    AGCTCAGGAG CTGGTGTTGT TAAGATGGGG AGATTTCAGG TTTCAGTTAC AATGGATGAT
    GCCCAGAAAG AGCGTAAAAA TAGGTCAGAA GATACAAAGT CGGTTCATTT TGAATCTAGT
    ACATCAGAGT CATCAGTTCT TTCAAGTAGT AGTCCAGAGA GTACCCTGGT AAAGCCAGAG
    CCTAATGGAA TTACTGTTTC TGGCATCTCC TTAGATGTGC CAGACAGTAC CCATAGAACA
    CCGACCCCAG AAGCAAAGTC AGAAACAGGA CAGCCTACCA AGGTTGGACG TTTTCAGGTG
    ACAACTACAG CCAACAAAGT GGGTCGTTTC TCTGTTTCAA GAACTGAGGA CAAGGTCACA
    GAGCTCAAAA AAGAGGGACC AGTGACCTCT CCTTTCAGAG ATTCAGAACA AACTGTTATT
    CCTGCTGCGA TACCAAAGAA GGAGAAGCCT GAGCTGGCAG AACCTTCCCA TCTGAATGGG
    CCATCTTCAG ACTTGGAGGC TGCCTTTCTA AGTAGGGGTG GAGAGGATGG ATCAGGTAGT
    CCTCACTCTC CCCCTCACCT GTGTTCAAAG AGCCTTCCCA TCCAGACTCT AAGTCAAAGC
    CTTAGTAATT CATTCAACTC CTCTTACATG AGTAGTGACA ATGAGTCCGA CATTGAAGAT
    GAAGACTTAA GGTTAGAACT GCGACGACTA CGGGAAAAAC ATCTTAAAGA GATTCAGGAC
    CTGCAGAGTC GCCAGAAGCA TGAAATTGAA TCTTTGTATA CCAAACTGGG CAAGGTTCCC
    CCTGCTGTCA TTATTCCCCC AGCTGCTCCT CTGTCAGGGA GAAGAAGGAG GCCTACTAAA
    AGCAAAGGCA GCAAGTCCAG TCGCAGCAGC TCATTGGGCA ACAAAAGCCC TCAGCTTTCA
    GGTAACCTGT CTGGTCAGAG TGGAACTTCA GTCTTAAACC CCCAACAGAC CCTCCATCCC
    CCAGGCAATA CCCCAGAGAC TGGACACAAC CAGCTGTTAC AGCCTCTTAA GCCATCTCCC
    TCCAGTGACA ACCTCTATTC AGCCTTTACC AGTGATGGTG CCATTTCAGT ACCAAGCCTT
    TCTGCTCCAG GTCAAGGGAC CAGCAGCACA AACACCGTTG GAGGAACAGT GAGTAGCCAG
    GCTGCCCAGG CTCAGCCTCC TGCCATGACT TCCAGTAGGA AGGGCACATT CACAGATGAC
    CTGCACAAGT TGGTAGACAA CTGGGCCCGA GATGCCATGA ATCTTTCAGG CAGGAGAGGC
    AGCAAAGGAC ACATGAATTA TGAGGGCCCT GGAATGGCAA GGAAGTTCTC TGCTCCTGGG
    CAGCTGTGCA TTTCCATGAC CTCAAACATG GGTGGCTCTA CCCCCATCTC TGCAGCATCT
    GCTACCTCTC TAGGTCACTT CACCAAGTCC ATGTGCCCCC CACAGCAGTA TGGTTTTCCA
    GCTGCCCCAT TCGGCACTCA GTGGAGTGGA ACAGGTGGTC CAGCACCACA GCCACTTGGT
    CAGTTCCAAC CTGTAGGAAC CACATCCTTG CAAAATTTCA ACATCAGCAA TTTGCAGAAG
    TCCATCAGCA ACCCCCCAGG TTCCAACCTA CGGACCACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.