WNK1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011521002.2	XP_011519304.1	serine/threonine-protein kinase WNK1 isoform X6
NM_018979.3	NP_061852.3	serine/threonine-protein kinase WNK1 isoform 1
NM_213655.4	NP_998820.3	serine/threonine-protein kinase WNK1 isoform 3
NM_001184985.1	NP_001171914.1	serine/threonine-protein kinase WNK1 isoform 4
XM_011521003.2	XP_011519305.1	serine/threonine-protein kinase WNK1 isoform X7
XM_011521005.2	XP_011519307.1	serine/threonine-protein kinase WNK1 isoform X9
XM_017019836.1	XP_016875325.1	serine/threonine-protein kinase WNK1 isoform X15
XM_017019839.1	XP_016875328.1	serine/threonine-protein kinase WNK1 isoform X20
NM_014823.2	NP_055638.2	serine/threonine-protein kinase WNK1 isoform 2
XM_011521004.2	XP_011519306.1	serine/threonine-protein kinase WNK1 isoform X8
XM_017019837.1	XP_016875326.1	serine/threonine-protein kinase WNK1 isoform X16
XM_017019838.1	XP_016875327.1	serine/threonine-protein kinase WNK1 isoform X17
XM_011521009.2	XP_011519311.1	serine/threonine-protein kinase WNK1 isoform X19
XM_011520997.2	XP_011519299.1	serine/threonine-protein kinase WNK1 isoform X1
XM_011520997.3	XP_011519299.1	serine/threonine-protein kinase WNK1 isoform X1
XM_011520998.2	XP_011519300.1	serine/threonine-protein kinase WNK1 isoform X2
XM_011520999.2	XP_011519301.1	serine/threonine-protein kinase WNK1 isoform X3
XM_011521000.2	XP_011519302.1	serine/threonine-protein kinase WNK1 isoform X4
XM_011521001.2	XP_011519303.1	serine/threonine-protein kinase WNK1 isoform X5
XM_006719003.2	XP_006719066.1	serine/threonine-protein kinase WNK1 isoform X10
XM_011521006.2	XP_011519308.1	serine/threonine-protein kinase WNK1 isoform X11
XM_017019835.1	XP_016875324.1	serine/threonine-protein kinase WNK1 isoform X14
XM_011521008.2	XP_011519310.1	serine/threonine-protein kinase WNK1 isoform X18
NM_014823.3	NP_055638.2	serine/threonine-protein kinase WNK1 isoform 2
XM_011521007.2	XP_011519309.1	serine/threonine-protein kinase WNK1 isoform X12
XM_017019834.1	XP_016875323.1	serine/threonine-protein kinase WNK1 isoform X13
NM_018979.4	NP_061852.3	serine/threonine-protein kinase WNK1 isoform 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu84074	XM_017019834.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu84075	XM_017019835.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu84076	XM_017019836.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu84077	XM_017019837.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu84078	XM_017019838.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu84079	XM_017019839.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu28095	NM_213655.4 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28140	NM_001184985.1 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28145	NM_014823.3 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28188	NM_018979.4 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu46009	XM_006719003.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu71618	XM_011521002.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu71619	XM_011521003.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu71620	XM_011521004.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu71621	XM_011521005.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu71622	XM_011521006.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu71623	XM_011521007.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu71624	XM_011521008.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu71625	XM_011521009.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu71613	XM_011520997.3 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu71614	XM_011520998.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu71615	XM_011520999.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu71616	XM_011521000.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu71617	XM_011521001.2 Latest version!	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu71613	XM_011520997.2	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu28145	NM_014823.2	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu28188	NM_018979.3	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
Hide Old Accession Versions >>

WNK1 ( XM_017019834.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875323.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu84074
Clone ID Related Accession (Same CDS sequence)	XM_017019834.1
Accession Version	XM_017019834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG GGCTTCCCAC CTCGACTGCC ACCACAGTAC 
CCAGGAGATT CAAATATTGC TCCCTCTTCC AACGTGGCTT CTGTTTGCAT CCATTCTACA 
GTCCTATCCC CTCCCATGCC GACAGAAGTA CTGGCTACAC CTGGGTACTT TCCCACAGTG 
GTGCAGCCTT ATGTGGAATC AAATCTTTTA GTTCCTATGG GTGGTGTAGG AGGACAGGTT 
CAAGTGTCCC AGCCAGGAGG GAGTTTAGCA CAAGCCCCCA CTACATCCTC CCAGCAAGCA 
GTTTTGGAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA 
CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA 
GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA 
ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA 
TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC 
AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA 
GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC 
TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT 
TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG 
GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC 
CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG 
TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA 
ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA 
GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA 
CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG 
GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT 
TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA 
AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC 
ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA 
GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA 
GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC 
ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT 
ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA 
GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA 
GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA 
CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT 
TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC 
TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT 
ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC 
AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT 
TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA 
CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC 
AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG 
GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG 
GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG 
AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT 
TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA 
AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG 
CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC 
CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG 
TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA 
GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA 
CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA 
AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG 
GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT 
CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT 
AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA 
GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG 
AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT 
CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA 
TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT 
CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA 
GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC 
TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA 
GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG 
AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC 
ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC 
GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGCTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875323.1
CDS	969..7655
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_017019834.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019834.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG GGCTTCCCAC CTCGACTGCC ACCACAGTAC 
CCAGGAGATT CAAATATTGC TCCCTCTTCC AACGTGGCTT CTGTTTGCAT CCATTCTACA 
GTCCTATCCC CTCCCATGCC GACAGAAGTA CTGGCTACAC CTGGGTACTT TCCCACAGTG 
GTGCAGCCTT ATGTGGAATC AAATCTTTTA GTTCCTATGG GTGGTGTAGG AGGACAGGTT 
CAAGTGTCCC AGCCAGGAGG GAGTTTAGCA CAAGCCCCCA CTACATCCTC CCAGCAAGCA 
GTTTTGGAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA 
CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA 
GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA 
ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA 
TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC 
AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA 
GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC 
TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT 
TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG 
GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC 
CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG 
TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA 
ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA 
GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA 
CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG 
GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT 
TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA 
AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC 
ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA 
GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA 
GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC 
ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT 
ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA 
GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA 
GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA 
CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT 
TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC 
TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT 
ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC 
AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT 
TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA 
CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC 
AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG 
GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG 
GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG 
AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT 
TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA 
AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG 
CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC 
CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG 
TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA 
GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA 
CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA 
AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG 
GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT 
CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT 
AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA 
GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG 
AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT 
CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA 
TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT 
CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA 
GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC 
TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA 
GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG 
AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC 
ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC 
GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGCTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_017019835.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875324.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.

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CloneID	OHu84075
Clone ID Related Accession (Same CDS sequence)	XM_017019835.1
Accession Version	XM_017019835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGG GCTTCCCACC TCGACTGCCA CCACAGTACC CAGGAGATTC AAATATTGCT 
CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTATCCCC TCCCATGCCG 
ACAGAAGTAC TGGCTACACC TGGGTACTTT CCCACAGTGG TGCAGCCTTA TGTGGAATCA 
AATCTTTTAG TTCCTATGGG TGGTGTAGGA GGACAGGTTC AAGTGTCCCA GCCAGGAGGG 
AGTTTAGCAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG TTTTGGAGAG TACTCAGGGA 
GTCTCTCAGG TTGCTCCTGC AGAGCCAGTT GCAGTAGCAC AGACCCAAGC TACCCAGCCG 
ACCACTTTGG CTTCCTCTGT AGACAGTGCA CATTCAGATG TTGCTTCAGG TATGAGTGAT 
GGCAATGAGA ACGTCCCATC TTCCAGTGGA AGGCATGAAG GAAGAACTAC AAAACGGCAT 
TACCGAAAAT CTGTAAGGAG TCGCTCTCGA CATGAAAAAA CTTCACGCCC AAAATTAAGA 
ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAATTAGA GACTCATAAT 
AGGAAAATGG TTACATTCAA ATTTGACCTA GATGGTGACA ACCCCGAGGA GATAGCAACA 
ATTATGGTGA ACAATGACTT TATTCTAGCA ATAGAGAGAG AGTCGTTTGT GGATCAAGTG 
CGAGAAATTA TTGAAAAAGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG 
GGTGATCAGG GATTGGAGAG TCTACAAGGA AAGGATGACT ATGGCTTTTC AGGTTCTCAG 
AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCGTCTT CCATGCCACA GCAAATAGGC 
ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCGG GAAGGCGGTT TATAGTGAGT 
CCTGTGCCAG AAAGCCGATT ACGAGAATCA AAAGTTTTCC CCAGTGAAAT AACAGATACA 
GTTGCTGCCT CTACAGCTCA GAGCCCTGGA ATGAACTTGT CTCACTCTGC ATCATCCCTT 
AGTCTACAAC AGGCCTTTTC TGAACTTAGA CGTGCCCAAA TGACAGAAGG ACCCAACACA 
GCACCTCCAA ACTTTAGTCA TACAGGACCA ACATTTCCAG TAGTACCTCC TTTCTTAAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGCA GCCACAGCAC CAGTCCCTGC AACAAGCAGC 
CCTCCTAATG ACATTTCCAC ATCAGTAATT CAGTCTGAGG TTACAGTGCC CACTGAAGAG 
GGGATTGCTG GAGTTGCCAC CAGCACAGGT GTGGTAACTT CAGGTGGTCT CCCCATACCA 
CCTGTGTCTG AATCACCAGT ACTTTCCAGC GTAGTTTCAA GTATCACAAT ACCTGCAGTT 
GTCTCAATAT CTACTACATC CCCGTCACTT CAAGTCCCCA CATCCACATC TGAGATCGTT 
GTTTCTAGTA CAGCACTGTA TCCTTCAGTA ACAGTTTCAG CAACTTCAGC CTCTGCAGGG 
GGCAGTACTG CTACCCCAGG TCCTAAGCCT CCAGCTGTAG TATCTCAGCA GGCAGCAGGC 
AGCACTACTG TGGGAGCCAC ATTAACATCA GTTTCTACCA CCACTTCATT CCCAAGCACA 
GCTTCACAGC TGTGCATTCA GCTTAGCAGC AGTACTTCTA CTCCTACTTT AGCTGAAACC 
GTGGTAGTTA GCGCACACTC ACTAGATAAG ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTCTCCCTCT CTGCACCATC TTCCTCTTCC TCTCCTGGAG CAGGAGTGTC TAGTTATATT 
TCTCAGCCTG GTGGGCTGCA TCCTTTGGTC ATTCCATCAG TGATAGCTTC TACTCCTATT 
CTTCCCCAAG CAGCAGGACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAGTATCCCA 
CCCTTGGTAC AGCCTGTTGC CAATGTGCCT GCTGTACAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTGCTTCC CAACCAGCCC CATACTCATT GTCCTGAAGT AGATTCTGAT 
ACACAACCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAAGAAAA GCTGCGGTCT 
CTGTTCAGTG AACACAGCTC ATCTGGAGCT CAGCATGCCT CTGTCTCACT GGAGACCTCA 
CTAGTCATAG AGAGCACTGT CACACCAGGC ATCCCAACTA CTGCTGTTGC ACCAAGCAAA 
CTCCTGACTT CTACCACAAG TACTTGCTTA CCACCAACCA ATTTACCACT AGGAACAGTT 
GCTTTGCCAG TTACACCAGT GGTCACACCT GGGCAAGTTT CTACCCCAGT CAGCACTACT 
ACATCAGGAG TGAAACCTGG AACTGCTCCC TCCAAGCCAC CTCTAACTAA GGCTCCGGTG 
CTGCCAGTGG GTACTGAACT TCCAGCAGGT ACTCTACCCA GCGAGCAGCT GCCACCTTTT 
CCAGGACCTT CTCTAACCCA GTCCCAGCAA CCTCTAGAGG ATCTTGATGC TCAATTGAGA 
AGAACACTTA GTCCAGAGAT GATCACAGTG ACTTCTGCGG TTGGTCCTGT GTCCATGGCG 
GCTCCAACAG CAATCACAGA AGCAGGAACA CAGCCTCAGA AGGGTGTTTC TCAAGTCAAA 
GAAGGCCCTG TCCTAGCAAC TAGTTCAGGA GCTGGTGTTT TTAAGATGGG ACGATTTCAG 
GTTTCTGTTG CAGCAGACGG TGCCCAGAAA GAGGGTAAAA ATAAGTCAGA AGATGCAAAG 
TCTGTTCATT TTGAATCCAG CACCTCAGAG TCCTCAGTGC TATCAAGTAG TAGTCCAGAG 
AGTACCTTGG TGAAACCAGA GCCGAATGGC ATAACCATCC CTGGTATCTC TTCAGATGTG 
CCAGAGAGTG CCCACAAAAC TACTGCCTCA GAGGCAAAGT CAGACACTGG GCAGCCTACC 
AAGGTTGGAC GTTTTCAGGT GACAACTACA GCAAACAAAG TGGGTCGTTT CTCTGTATCA 
AAAACTGAGG ACAAGATCAC TGACACAAAG AAAGAAGGAC CAGTGGCATC TCCTCCTTTT 
ATGGATTTGG AACAAGCTGT TCTTCCTGCT GTGATACCAA AGAAAGAGAA GCCTGAACTG 
TCAGAGCCTT CACATCTAAA TGGGCCGTCT TCTGACCCGG AGGCCGCTTT TTTAAGTAGG 
GATGTGGATG ATGGTTCCGG TAGTCCACAC TCGCCCCATC AGCTGAGCTC AAAGAGCCTT 
CCTAGCCAGA ATCTAAGTCA AAGCCTTAGT AATTCATTTA ACTCCTCTTA CATGAGTAGC 
GACAATGAGT CAGATATCGA AGATGAAGAC TTAAAGTTAG AGCTGCGACG ACTACGAGAT 
AAACATCTCA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG 
TATACCAAAC TGGGCAAGGT GCCCCCTGCT GTTATTATTC CCCCAGCTGC TCCCCTTTCA 
GGGAGAAGAC GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGTCGAAG CAGTTCCTTG 
GGGAATAAAA GCCCCCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGCAGC TTCAGTCTTG 
CACCCCCAGC AGACCCTCCA CCCTCCTGGC AACATCCCAG AGTCCGGGCA GAATCAGCTG 
TTACAGCCCC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT CACCAGTGAT 
GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GAACCAGCAG CACAAACACT 
GTTGGGGCAA CAGTGAACAG CCAAGCCGCC CAAGCTCAGC CTCCTGCCAT GACGTCCAGC 
AGGAAGGGCA CATTCACAGA TGACTTGCAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC 
ATGAATCTCT CAGGCAGGAG AGGAAGCAAA GGGCACATGA ATTACGAGGG CCCTGGAATG 
GCAAGGAAGT TCTCTGCACC TGGGCAACTG TGCATCTCCA TGACCTCGAA CCTGGGTGGC 
TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC 
CCCCCACAGC AGTATGGCTT TCCAGCTACC CCATTTGGCG CTCAATGGAG TGGGACGGGT 
GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTGCAGAAT 
TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGCTCCAA CCTGCGGACC 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875324.1
CDS	969..7574
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_017019835.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019835.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGG GCTTCCCACC TCGACTGCCA CCACAGTACC CAGGAGATTC AAATATTGCT 
CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTATCCCC TCCCATGCCG 
ACAGAAGTAC TGGCTACACC TGGGTACTTT CCCACAGTGG TGCAGCCTTA TGTGGAATCA 
AATCTTTTAG TTCCTATGGG TGGTGTAGGA GGACAGGTTC AAGTGTCCCA GCCAGGAGGG 
AGTTTAGCAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG TTTTGGAGAG TACTCAGGGA 
GTCTCTCAGG TTGCTCCTGC AGAGCCAGTT GCAGTAGCAC AGACCCAAGC TACCCAGCCG 
ACCACTTTGG CTTCCTCTGT AGACAGTGCA CATTCAGATG TTGCTTCAGG TATGAGTGAT 
GGCAATGAGA ACGTCCCATC TTCCAGTGGA AGGCATGAAG GAAGAACTAC AAAACGGCAT 
TACCGAAAAT CTGTAAGGAG TCGCTCTCGA CATGAAAAAA CTTCACGCCC AAAATTAAGA 
ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAATTAGA GACTCATAAT 
AGGAAAATGG TTACATTCAA ATTTGACCTA GATGGTGACA ACCCCGAGGA GATAGCAACA 
ATTATGGTGA ACAATGACTT TATTCTAGCA ATAGAGAGAG AGTCGTTTGT GGATCAAGTG 
CGAGAAATTA TTGAAAAAGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG 
GGTGATCAGG GATTGGAGAG TCTACAAGGA AAGGATGACT ATGGCTTTTC AGGTTCTCAG 
AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCGTCTT CCATGCCACA GCAAATAGGC 
ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCGG GAAGGCGGTT TATAGTGAGT 
CCTGTGCCAG AAAGCCGATT ACGAGAATCA AAAGTTTTCC CCAGTGAAAT AACAGATACA 
GTTGCTGCCT CTACAGCTCA GAGCCCTGGA ATGAACTTGT CTCACTCTGC ATCATCCCTT 
AGTCTACAAC AGGCCTTTTC TGAACTTAGA CGTGCCCAAA TGACAGAAGG ACCCAACACA 
GCACCTCCAA ACTTTAGTCA TACAGGACCA ACATTTCCAG TAGTACCTCC TTTCTTAAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGCA GCCACAGCAC CAGTCCCTGC AACAAGCAGC 
CCTCCTAATG ACATTTCCAC ATCAGTAATT CAGTCTGAGG TTACAGTGCC CACTGAAGAG 
GGGATTGCTG GAGTTGCCAC CAGCACAGGT GTGGTAACTT CAGGTGGTCT CCCCATACCA 
CCTGTGTCTG AATCACCAGT ACTTTCCAGC GTAGTTTCAA GTATCACAAT ACCTGCAGTT 
GTCTCAATAT CTACTACATC CCCGTCACTT CAAGTCCCCA CATCCACATC TGAGATCGTT 
GTTTCTAGTA CAGCACTGTA TCCTTCAGTA ACAGTTTCAG CAACTTCAGC CTCTGCAGGG 
GGCAGTACTG CTACCCCAGG TCCTAAGCCT CCAGCTGTAG TATCTCAGCA GGCAGCAGGC 
AGCACTACTG TGGGAGCCAC ATTAACATCA GTTTCTACCA CCACTTCATT CCCAAGCACA 
GCTTCACAGC TGTGCATTCA GCTTAGCAGC AGTACTTCTA CTCCTACTTT AGCTGAAACC 
GTGGTAGTTA GCGCACACTC ACTAGATAAG ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTCTCCCTCT CTGCACCATC TTCCTCTTCC TCTCCTGGAG CAGGAGTGTC TAGTTATATT 
TCTCAGCCTG GTGGGCTGCA TCCTTTGGTC ATTCCATCAG TGATAGCTTC TACTCCTATT 
CTTCCCCAAG CAGCAGGACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAGTATCCCA 
CCCTTGGTAC AGCCTGTTGC CAATGTGCCT GCTGTACAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTGCTTCC CAACCAGCCC CATACTCATT GTCCTGAAGT AGATTCTGAT 
ACACAACCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAAGAAAA GCTGCGGTCT 
CTGTTCAGTG AACACAGCTC ATCTGGAGCT CAGCATGCCT CTGTCTCACT GGAGACCTCA 
CTAGTCATAG AGAGCACTGT CACACCAGGC ATCCCAACTA CTGCTGTTGC ACCAAGCAAA 
CTCCTGACTT CTACCACAAG TACTTGCTTA CCACCAACCA ATTTACCACT AGGAACAGTT 
GCTTTGCCAG TTACACCAGT GGTCACACCT GGGCAAGTTT CTACCCCAGT CAGCACTACT 
ACATCAGGAG TGAAACCTGG AACTGCTCCC TCCAAGCCAC CTCTAACTAA GGCTCCGGTG 
CTGCCAGTGG GTACTGAACT TCCAGCAGGT ACTCTACCCA GCGAGCAGCT GCCACCTTTT 
CCAGGACCTT CTCTAACCCA GTCCCAGCAA CCTCTAGAGG ATCTTGATGC TCAATTGAGA 
AGAACACTTA GTCCAGAGAT GATCACAGTG ACTTCTGCGG TTGGTCCTGT GTCCATGGCG 
GCTCCAACAG CAATCACAGA AGCAGGAACA CAGCCTCAGA AGGGTGTTTC TCAAGTCAAA 
GAAGGCCCTG TCCTAGCAAC TAGTTCAGGA GCTGGTGTTT TTAAGATGGG ACGATTTCAG 
GTTTCTGTTG CAGCAGACGG TGCCCAGAAA GAGGGTAAAA ATAAGTCAGA AGATGCAAAG 
TCTGTTCATT TTGAATCCAG CACCTCAGAG TCCTCAGTGC TATCAAGTAG TAGTCCAGAG 
AGTACCTTGG TGAAACCAGA GCCGAATGGC ATAACCATCC CTGGTATCTC TTCAGATGTG 
CCAGAGAGTG CCCACAAAAC TACTGCCTCA GAGGCAAAGT CAGACACTGG GCAGCCTACC 
AAGGTTGGAC GTTTTCAGGT GACAACTACA GCAAACAAAG TGGGTCGTTT CTCTGTATCA 
AAAACTGAGG ACAAGATCAC TGACACAAAG AAAGAAGGAC CAGTGGCATC TCCTCCTTTT 
ATGGATTTGG AACAAGCTGT TCTTCCTGCT GTGATACCAA AGAAAGAGAA GCCTGAACTG 
TCAGAGCCTT CACATCTAAA TGGGCCGTCT TCTGACCCGG AGGCCGCTTT TTTAAGTAGG 
GATGTGGATG ATGGTTCCGG TAGTCCACAC TCGCCCCATC AGCTGAGCTC AAAGAGCCTT 
CCTAGCCAGA ATCTAAGTCA AAGCCTTAGT AATTCATTTA ACTCCTCTTA CATGAGTAGC 
GACAATGAGT CAGATATCGA AGATGAAGAC TTAAAGTTAG AGCTGCGACG ACTACGAGAT 
AAACATCTCA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG 
TATACCAAAC TGGGCAAGGT GCCCCCTGCT GTTATTATTC CCCCAGCTGC TCCCCTTTCA 
GGGAGAAGAC GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGTCGAAG CAGTTCCTTG 
GGGAATAAAA GCCCCCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGCAGC TTCAGTCTTG 
CACCCCCAGC AGACCCTCCA CCCTCCTGGC AACATCCCAG AGTCCGGGCA GAATCAGCTG 
TTACAGCCCC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT CACCAGTGAT 
GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GAACCAGCAG CACAAACACT 
GTTGGGGCAA CAGTGAACAG CCAAGCCGCC CAAGCTCAGC CTCCTGCCAT GACGTCCAGC 
AGGAAGGGCA CATTCACAGA TGACTTGCAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC 
ATGAATCTCT CAGGCAGGAG AGGAAGCAAA GGGCACATGA ATTACGAGGG CCCTGGAATG 
GCAAGGAAGT TCTCTGCACC TGGGCAACTG TGCATCTCCA TGACCTCGAA CCTGGGTGGC 
TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC 
CCCCCACAGC AGTATGGCTT TCCAGCTACC CCATTTGGCG CTCAATGGAG TGGGACGGGT 
GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTGCAGAAT 
TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGCTCCAA CCTGCGGACC 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_017019836.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875325.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.

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CloneID	OHu84076
Clone ID Related Accession (Same CDS sequence)	XM_017019836.1
Accession Version	XM_017019836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC 
CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT 
ACTGCACAGC CAGTGAGTCA GCCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA 
AAACAGGGCT TCCCACCTCG ACTGCCACCA CAGTACCCAG GAGATTCAAA TATTGCTCCC 
TCTTCCAACG TGGCTTCTGT TTGCATCCAT TCTACAGTCC TATCCCCTCC CATGCCGACA 
GAAGTACTGG CTACACCTGG GTACTTTCCC ACAGTGGTGC AGCCTTATGT GGAATCAAAT 
CTTTTAGTTC CTATGGGTGG TGTAGGAGGA CAGGTTCAAG TGTCCCAGCC AGGAGGGAGT 
TTAGCACAAG CCCCCACTAC ATCCTCCCAG CAAGCAGTTT TGGAGAGTAC TCAGGGAGTC 
TCTCAGGTTG CTCCTGCAGA GCCAGTTGCA GTAGCACAGA CCCAAGCTAC CCAGCCGACC 
ACTTTGGCTT CCTCTGTAGA CAGTGCACAT TCAGATGTTG CTTCAGGTAT GAGTGATGGC 
AATGAGAACG TCCCATCTTC CAGTGGAAGG CATGAAGGAA GAACTACAAA ACGGCATTAC 
CGAAAATCTG TAAGGAGTCG CTCTCGACAT GAAAAAACTT CACGCCCAAA ATTAAGAATT 
TTGAATGTTT CAAATAAAGG AGACCGAGTA GTAGAATGTC AATTAGAGAC TCATAATAGG 
AAAATGGTTA CATTCAAATT TGACCTAGAT GGTGACAACC CCGAGGAGAT AGCAACAATT 
ATGGTGAACA ATGACTTTAT TCTAGCAATA GAGAGAGAGT CGTTTGTGGA TCAAGTGCGA 
GAAATTATTG AAAAAGCTGA TGAAATGCTC AGTGAGGATG TCAGTGTGGA ACCAGAGGGT 
GATCAGGGAT TGGAGAGTCT ACAAGGAAAG GATGACTATG GCTTTTCAGG TTCTCAGAAA 
TTGGAAGGAG AGTTCAAACA ACCAATTCCT GCGTCTTCCA TGCCACAGCA AATAGGCATT 
CCTACCAGTT CTTTAACTCA AGTTGTTCAT TCTGCGGGAA GGCGGTTTAT AGTGAGTCCT 
GTGCCAGAAA GCCGATTACG AGAATCAAAA GTTTTCCCCA GTGAAATAAC AGATACAGTT 
GCTGCCTCTA CAGCTCAGAG CCCTGGAATG AACTTGTCTC ACTCTGCATC ATCCCTTAGT 
CTACAACAGG CCTTTTCTGA ACTTAGACGT GCCCAAATGA CAGAAGGACC CAACACAGCA 
CCTCCAAACT TTAGTCATAC AGGACCAACA TTTCCAGTAG TACCTCCTTT CTTAAGTAGC 
ATTGCTGGAG TCCCAACCAC AGCAGCAGCC ACAGCACCAG TCCCTGCAAC AAGCAGCCCT 
CCTAATGACA TTTCCACATC AGTAATTCAG TCTGAGGTTA CAGTGCCCAC TGAAGAGGGG 
ATTGCTGGAG TTGCCACCAG CACAGGTGTG GTAACTTCAG GTGGTCTCCC CATACCACCT 
GTGTCTGAAT CACCAGTACT TTCCAGCGTA GTTTCAAGTA TCACAATACC TGCAGTTGTC 
TCAATATCTA CTACATCCCC GTCACTTCAA GTCCCCACAT CCACATCTGA GATCGTTGTT 
TCTAGTACAG CACTGTATCC TTCAGTAACA GTTTCAGCAA CTTCAGCCTC TGCAGGGGGC 
AGTACTGCTA CCCCAGGTCC TAAGCCTCCA GCTGTAGTAT CTCAGCAGGC AGCAGGCAGC 
ACTACTGTGG GAGCCACATT AACATCAGTT TCTACCACCA CTTCATTCCC AAGCACAGCT 
TCACAGCTGT GCATTCAGCT TAGCAGCAGT ACTTCTACTC CTACTTTAGC TGAAACCGTG 
GTAGTTAGCG CACACTCACT AGATAAGACA TCTCATAGCA GTACAACTGG ATTGGCTTTC 
TCCCTCTCTG CACCATCTTC CTCTTCCTCT CCTGGAGCAG GAGTGTCTAG TTATATTTCT 
CAGCCTGGTG GGCTGCATCC TTTGGTCATT CCATCAGTGA TAGCTTCTAC TCCTATTCTT 
CCCCAAGCAG CAGGACCTAC TTCTACACCT TTATTACCCC AAGTACCTAG TATCCCACCC 
TTGGTACAGC CTGTTGCCAA TGTGCCTGCT GTACAGCAGA CACTAATTCA TAGTCAGCCT 
CAACCAGCTT TGCTTCCCAA CCAGCCCCAT ACTCATTGTC CTGAAGTAGA TTCTGATACA 
CAACCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AAGAAAAGCT GCGGTCTCTG 
TTCAGTGAAC ACAGCTCATC TGGAGCTCAG CATGCCTCTG TCTCACTGGA GACCTCACTA 
GTCATAGAGA GCACTGTCAC ACCAGGCATC CCAACTACTG CTGTTGCACC AAGCAAACTC 
CTGACTTCTA CCACAAGTAC TTGCTTACCA CCAACCAATT TACCACTAGG AACAGTTGCT 
TTGCCAGTTA CACCAGTGGT CACACCTGGG CAAGTTTCTA CCCCAGTCAG CACTACTACA 
TCAGGAGTGA AACCTGGAAC TGCTCCCTCC AAGCCACCTC TAACTAAGGC TCCGGTGCTG 
CCAGTGGGTA CTGAACTTCC AGCAGGTACT CTACCCAGCG AGCAGCTGCC ACCTTTTCCA 
GGACCTTCTC TAACCCAGTC CCAGCAACCT CTAGAGGATC TTGATGCTCA ATTGAGAAGA 
ACACTTAGTC CAGAGATGAT CACAGTGACT TCTGCGGTTG GTCCTGTGTC CATGGCGGCT 
CCAACAGCAA TCACAGAAGC AGGAACACAG CCTCAGAAGG GTGTTTCTCA AGTCAAAGAA 
GGCCCTGTCC TAGCAACTAG TTCAGGAGCT GGTGTTTTTA AGATGGGACG ATTTCAGGTT 
TCTGTTGCAG CAGACGGTGC CCAGAAAGAG GGTAAAAATA AGTCAGAAGA TGCAAAGTCT 
GTTCATTTTG AATCCAGCAC CTCAGAGTCC TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT 
ACCTTGGTGA AACCAGAGCC GAATGGCATA ACCATCCCTG GTATCTCTTC AGATGTGCCA 
GAGAGTGCCC ACAAAACTAC TGCCTCAGAG GCAAAGTCAG ACACTGGGCA GCCTACCAAG 
GTTGGACGTT TTCAGGTGAC AACTACAGCA AACAAAGTGG GTCGTTTCTC TGTATCAAAA 
ACTGAGGACA AGATCACTGA CACAAAGAAA GAAGGACCAG TGGCATCTCC TCCTTTTATG 
GATTTGGAAC AAGCTGTTCT TCCTGCTGTG ATACCAAAGA AAGAGAAGCC TGAACTGTCA 
GAGCCTTCAC ATCTAAATGG GCCGTCTTCT GACCCGGAGG CCGCTTTTTT AAGTAGGGAT 
GTGGATGATG GTTCCGGTAG TCCACACTCG CCCCATCAGC TGAGCTCAAA GAGCCTTCCT 
AGCCAGAATC TAAGTCAAAG CCTTAGTAAT TCATTTAACT CCTCTTACAT GAGTAGCGAC 
AATGAGTCAG ATATCGAAGA TGAAGACTTA AAGTTAGAGC TGCGACGACT ACGAGATAAA 
CATCTCAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT 
ACCAAACTGG GCAAGGTGCC CCCTGCTGTT ATTATTCCCC CAGCTGCTCC CCTTTCAGGG 
AGAAGACGAC GACCCACTAA AAGCAAAGGC AGCAAATCTA GTCGAAGCAG TTCCTTGGGG 
AATAAAAGCC CCCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGCAGCTTC AGTCTTGCAC 
CCCCAGCAGA CCCTCCACCC TCCTGGCAAC ATCCCAGAGT CCGGGCAGAA TCAGCTGTTA 
CAGCCCCTTA AGCCATCTCC CTCCAGTGAC AACCTCTATT CAGCCTTCAC CAGTGATGGT 
GCCATTTCAG TACCAAGCCT TTCTGCTCCA GGTCAAGGAA CCAGCAGCAC AAACACTGTT 
GGGGCAACAG TGAACAGCCA AGCCGCCCAA GCTCAGCCTC CTGCCATGAC GTCCAGCAGG 
AAGGGCACAT TCACAGATGA CTTGCACAAG TTGGTAGACA ATTGGGCCCG AGATGCCATG 
AATCTCTCAG GCAGGAGAGG AAGCAAAGGG CACATGAATT ACGAGGGCCC TGGAATGGCA 
AGGAAGTTCT CTGCACCTGG GCAACTGTGC ATCTCCATGA CCTCGAACCT GGGTGGCTCT 
GCCCCCATCT CTGCAGCATC AGCTACCTCT CTAGGTCACT TCACCAAGTC TATGTGCCCC 
CCACAGCAGT ATGGCTTTCC AGCTACCCCA TTTGGCGCTC AATGGAGTGG GACGGGTGGC 
CCAGCACCAC AGCCACTTGG CCAGTTCCAA CCTGTGGGAA CTGCCTCCTT GCAGAATTTC 
AACATCAGCA ATTTGCAGAA ATCCATCAGC AACCCCCCAG GCTCCAACCT GCGGACCACT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875325.1
CDS	969..7571
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_017019836.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019836.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC 
CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT 
ACTGCACAGC CAGTGAGTCA GCCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA 
AAACAGGGCT TCCCACCTCG ACTGCCACCA CAGTACCCAG GAGATTCAAA TATTGCTCCC 
TCTTCCAACG TGGCTTCTGT TTGCATCCAT TCTACAGTCC TATCCCCTCC CATGCCGACA 
GAAGTACTGG CTACACCTGG GTACTTTCCC ACAGTGGTGC AGCCTTATGT GGAATCAAAT 
CTTTTAGTTC CTATGGGTGG TGTAGGAGGA CAGGTTCAAG TGTCCCAGCC AGGAGGGAGT 
TTAGCACAAG CCCCCACTAC ATCCTCCCAG CAAGCAGTTT TGGAGAGTAC TCAGGGAGTC 
TCTCAGGTTG CTCCTGCAGA GCCAGTTGCA GTAGCACAGA CCCAAGCTAC CCAGCCGACC 
ACTTTGGCTT CCTCTGTAGA CAGTGCACAT TCAGATGTTG CTTCAGGTAT GAGTGATGGC 
AATGAGAACG TCCCATCTTC CAGTGGAAGG CATGAAGGAA GAACTACAAA ACGGCATTAC 
CGAAAATCTG TAAGGAGTCG CTCTCGACAT GAAAAAACTT CACGCCCAAA ATTAAGAATT 
TTGAATGTTT CAAATAAAGG AGACCGAGTA GTAGAATGTC AATTAGAGAC TCATAATAGG 
AAAATGGTTA CATTCAAATT TGACCTAGAT GGTGACAACC CCGAGGAGAT AGCAACAATT 
ATGGTGAACA ATGACTTTAT TCTAGCAATA GAGAGAGAGT CGTTTGTGGA TCAAGTGCGA 
GAAATTATTG AAAAAGCTGA TGAAATGCTC AGTGAGGATG TCAGTGTGGA ACCAGAGGGT 
GATCAGGGAT TGGAGAGTCT ACAAGGAAAG GATGACTATG GCTTTTCAGG TTCTCAGAAA 
TTGGAAGGAG AGTTCAAACA ACCAATTCCT GCGTCTTCCA TGCCACAGCA AATAGGCATT 
CCTACCAGTT CTTTAACTCA AGTTGTTCAT TCTGCGGGAA GGCGGTTTAT AGTGAGTCCT 
GTGCCAGAAA GCCGATTACG AGAATCAAAA GTTTTCCCCA GTGAAATAAC AGATACAGTT 
GCTGCCTCTA CAGCTCAGAG CCCTGGAATG AACTTGTCTC ACTCTGCATC ATCCCTTAGT 
CTACAACAGG CCTTTTCTGA ACTTAGACGT GCCCAAATGA CAGAAGGACC CAACACAGCA 
CCTCCAAACT TTAGTCATAC AGGACCAACA TTTCCAGTAG TACCTCCTTT CTTAAGTAGC 
ATTGCTGGAG TCCCAACCAC AGCAGCAGCC ACAGCACCAG TCCCTGCAAC AAGCAGCCCT 
CCTAATGACA TTTCCACATC AGTAATTCAG TCTGAGGTTA CAGTGCCCAC TGAAGAGGGG 
ATTGCTGGAG TTGCCACCAG CACAGGTGTG GTAACTTCAG GTGGTCTCCC CATACCACCT 
GTGTCTGAAT CACCAGTACT TTCCAGCGTA GTTTCAAGTA TCACAATACC TGCAGTTGTC 
TCAATATCTA CTACATCCCC GTCACTTCAA GTCCCCACAT CCACATCTGA GATCGTTGTT 
TCTAGTACAG CACTGTATCC TTCAGTAACA GTTTCAGCAA CTTCAGCCTC TGCAGGGGGC 
AGTACTGCTA CCCCAGGTCC TAAGCCTCCA GCTGTAGTAT CTCAGCAGGC AGCAGGCAGC 
ACTACTGTGG GAGCCACATT AACATCAGTT TCTACCACCA CTTCATTCCC AAGCACAGCT 
TCACAGCTGT GCATTCAGCT TAGCAGCAGT ACTTCTACTC CTACTTTAGC TGAAACCGTG 
GTAGTTAGCG CACACTCACT AGATAAGACA TCTCATAGCA GTACAACTGG ATTGGCTTTC 
TCCCTCTCTG CACCATCTTC CTCTTCCTCT CCTGGAGCAG GAGTGTCTAG TTATATTTCT 
CAGCCTGGTG GGCTGCATCC TTTGGTCATT CCATCAGTGA TAGCTTCTAC TCCTATTCTT 
CCCCAAGCAG CAGGACCTAC TTCTACACCT TTATTACCCC AAGTACCTAG TATCCCACCC 
TTGGTACAGC CTGTTGCCAA TGTGCCTGCT GTACAGCAGA CACTAATTCA TAGTCAGCCT 
CAACCAGCTT TGCTTCCCAA CCAGCCCCAT ACTCATTGTC CTGAAGTAGA TTCTGATACA 
CAACCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AAGAAAAGCT GCGGTCTCTG 
TTCAGTGAAC ACAGCTCATC TGGAGCTCAG CATGCCTCTG TCTCACTGGA GACCTCACTA 
GTCATAGAGA GCACTGTCAC ACCAGGCATC CCAACTACTG CTGTTGCACC AAGCAAACTC 
CTGACTTCTA CCACAAGTAC TTGCTTACCA CCAACCAATT TACCACTAGG AACAGTTGCT 
TTGCCAGTTA CACCAGTGGT CACACCTGGG CAAGTTTCTA CCCCAGTCAG CACTACTACA 
TCAGGAGTGA AACCTGGAAC TGCTCCCTCC AAGCCACCTC TAACTAAGGC TCCGGTGCTG 
CCAGTGGGTA CTGAACTTCC AGCAGGTACT CTACCCAGCG AGCAGCTGCC ACCTTTTCCA 
GGACCTTCTC TAACCCAGTC CCAGCAACCT CTAGAGGATC TTGATGCTCA ATTGAGAAGA 
ACACTTAGTC CAGAGATGAT CACAGTGACT TCTGCGGTTG GTCCTGTGTC CATGGCGGCT 
CCAACAGCAA TCACAGAAGC AGGAACACAG CCTCAGAAGG GTGTTTCTCA AGTCAAAGAA 
GGCCCTGTCC TAGCAACTAG TTCAGGAGCT GGTGTTTTTA AGATGGGACG ATTTCAGGTT 
TCTGTTGCAG CAGACGGTGC CCAGAAAGAG GGTAAAAATA AGTCAGAAGA TGCAAAGTCT 
GTTCATTTTG AATCCAGCAC CTCAGAGTCC TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT 
ACCTTGGTGA AACCAGAGCC GAATGGCATA ACCATCCCTG GTATCTCTTC AGATGTGCCA 
GAGAGTGCCC ACAAAACTAC TGCCTCAGAG GCAAAGTCAG ACACTGGGCA GCCTACCAAG 
GTTGGACGTT TTCAGGTGAC AACTACAGCA AACAAAGTGG GTCGTTTCTC TGTATCAAAA 
ACTGAGGACA AGATCACTGA CACAAAGAAA GAAGGACCAG TGGCATCTCC TCCTTTTATG 
GATTTGGAAC AAGCTGTTCT TCCTGCTGTG ATACCAAAGA AAGAGAAGCC TGAACTGTCA 
GAGCCTTCAC ATCTAAATGG GCCGTCTTCT GACCCGGAGG CCGCTTTTTT AAGTAGGGAT 
GTGGATGATG GTTCCGGTAG TCCACACTCG CCCCATCAGC TGAGCTCAAA GAGCCTTCCT 
AGCCAGAATC TAAGTCAAAG CCTTAGTAAT TCATTTAACT CCTCTTACAT GAGTAGCGAC 
AATGAGTCAG ATATCGAAGA TGAAGACTTA AAGTTAGAGC TGCGACGACT ACGAGATAAA 
CATCTCAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT 
ACCAAACTGG GCAAGGTGCC CCCTGCTGTT ATTATTCCCC CAGCTGCTCC CCTTTCAGGG 
AGAAGACGAC GACCCACTAA AAGCAAAGGC AGCAAATCTA GTCGAAGCAG TTCCTTGGGG 
AATAAAAGCC CCCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGCAGCTTC AGTCTTGCAC 
CCCCAGCAGA CCCTCCACCC TCCTGGCAAC ATCCCAGAGT CCGGGCAGAA TCAGCTGTTA 
CAGCCCCTTA AGCCATCTCC CTCCAGTGAC AACCTCTATT CAGCCTTCAC CAGTGATGGT 
GCCATTTCAG TACCAAGCCT TTCTGCTCCA GGTCAAGGAA CCAGCAGCAC AAACACTGTT 
GGGGCAACAG TGAACAGCCA AGCCGCCCAA GCTCAGCCTC CTGCCATGAC GTCCAGCAGG 
AAGGGCACAT TCACAGATGA CTTGCACAAG TTGGTAGACA ATTGGGCCCG AGATGCCATG 
AATCTCTCAG GCAGGAGAGG AAGCAAAGGG CACATGAATT ACGAGGGCCC TGGAATGGCA 
AGGAAGTTCT CTGCACCTGG GCAACTGTGC ATCTCCATGA CCTCGAACCT GGGTGGCTCT 
GCCCCCATCT CTGCAGCATC AGCTACCTCT CTAGGTCACT TCACCAAGTC TATGTGCCCC 
CCACAGCAGT ATGGCTTTCC AGCTACCCCA TTTGGCGCTC AATGGAGTGG GACGGGTGGC 
CCAGCACCAC AGCCACTTGG CCAGTTCCAA CCTGTGGGAA CTGCCTCCTT GCAGAATTTC 
AACATCAGCA ATTTGCAGAA ATCCATCAGC AACCCCCCAG GCTCCAACCT GCGGACCACT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_017019837.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875326.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.

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CloneID	OHu84077
Clone ID Related Accession (Same CDS sequence)	XM_017019837.1
Accession Version	XM_017019837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAGCC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGAGTACTC AGGGAGTCTC TCAGGTTGCT 
CCTGCAGAGC CAGTTGCAGT AGCACAGACC CAAGCTACCC AGCCGACCAC TTTGGCTTCC 
TCTGTAGACA GTGCACATTC AGATGTTGCT TCAGGTATGA GTGATGGCAA TGAGAACGTC 
CCATCTTCCA GTGGAAGGCA TGAAGGAAGA ACTACAAAAC GGCATTACCG AAAATCTGTA 
AGGAGTCGCT CTCGACATGA AAAAACTTCA CGCCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA TTAGAGACTC ATAATAGGAA AATGGTTACA 
TTCAAATTTG ACCTAGATGG TGACAACCCC GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATAGA GAGAGAGTCG TTTGTGGATC AAGTGCGAGA AATTATTGAA 
AAAGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA TCAGGGATTG 
GAGAGTCTAC AAGGAAAGGA TGACTATGGC TTTTCAGGTT CTCAGAAATT GGAAGGAGAG 
TTCAAACAAC CAATTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCGGGAAGG CGGTTTATAG TGAGTCCTGT GCCAGAAAGC 
CGATTACGAG AATCAAAAGT TTTCCCCAGT GAAATAACAG ATACAGTTGC TGCCTCTACA 
GCTCAGAGCC CTGGAATGAA CTTGTCTCAC TCTGCATCAT CCCTTAGTCT ACAACAGGCC 
TTTTCTGAAC TTAGACGTGC CCAAATGACA GAAGGACCCA ACACAGCACC TCCAAACTTT 
AGTCATACAG GACCAACATT TCCAGTAGTA CCTCCTTTCT TAAGTAGCAT TGCTGGAGTC 
CCAACCACAG CAGCAGCCAC AGCACCAGTC CCTGCAACAA GCAGCCCTCC TAATGACATT 
TCCACATCAG TAATTCAGTC TGAGGTTACA GTGCCCACTG AAGAGGGGAT TGCTGGAGTT 
GCCACCAGCA CAGGTGTGGT AACTTCAGGT GGTCTCCCCA TACCACCTGT GTCTGAATCA 
CCAGTACTTT CCAGCGTAGT TTCAAGTATC ACAATACCTG CAGTTGTCTC AATATCTACT 
ACATCCCCGT CACTTCAAGT CCCCACATCC ACATCTGAGA TCGTTGTTTC TAGTACAGCA 
CTGTATCCTT CAGTAACAGT TTCAGCAACT TCAGCCTCTG CAGGGGGCAG TACTGCTACC 
CCAGGTCCTA AGCCTCCAGC TGTAGTATCT CAGCAGGCAG CAGGCAGCAC TACTGTGGGA 
GCCACATTAA CATCAGTTTC TACCACCACT TCATTCCCAA GCACAGCTTC ACAGCTGTGC 
ATTCAGCTTA GCAGCAGTAC TTCTACTCCT ACTTTAGCTG AAACCGTGGT AGTTAGCGCA 
CACTCACTAG ATAAGACATC TCATAGCAGT ACAACTGGAT TGGCTTTCTC CCTCTCTGCA 
CCATCTTCCT CTTCCTCTCC TGGAGCAGGA GTGTCTAGTT ATATTTCTCA GCCTGGTGGG 
CTGCATCCTT TGGTCATTCC ATCAGTGATA GCTTCTACTC CTATTCTTCC CCAAGCAGCA 
GGACCTACTT CTACACCTTT ATTACCCCAA GTACCTAGTA TCCCACCCTT GGTACAGCCT 
GTTGCCAATG TGCCTGCTGT ACAGCAGACA CTAATTCATA GTCAGCCTCA ACCAGCTTTG 
CTTCCCAACC AGCCCCATAC TCATTGTCCT GAAGTAGATT CTGATACACA ACCCAAAGCT 
CCTGGAATTG ATGACATAAA GACTCTAGAA GAAAAGCTGC GGTCTCTGTT CAGTGAACAC 
AGCTCATCTG GAGCTCAGCA TGCCTCTGTC TCACTGGAGA CCTCACTAGT CATAGAGAGC 
ACTGTCACAC CAGGCATCCC AACTACTGCT GTTGCACCAA GCAAACTCCT GACTTCTACC 
ACAAGTACTT GCTTACCACC AACCAATTTA CCACTAGGAA CAGTTGCTTT GCCAGTTACA 
CCAGTGGTCA CACCTGGGCA AGTTTCTACC CCAGTCAGCA CTACTACATC AGGAGTGAAA 
CCTGGAACTG CTCCCTCCAA GCCACCTCTA ACTAAGGCTC CGGTGCTGCC AGTGGGTACT 
GAACTTCCAG CAGGTACTCT ACCCAGCGAG CAGCTGCCAC CTTTTCCAGG ACCTTCTCTA 
ACCCAGTCCC AGCAACCTCT AGAGGATCTT GATGCTCAAT TGAGAAGAAC ACTTAGTCCA 
GAGATGATCA CAGTGACTTC TGCGGTTGGT CCTGTGTCCA TGGCGGCTCC AACAGCAATC 
ACAGAAGCAG GAACACAGCC TCAGAAGGGT GTTTCTCAAG TCAAAGAAGG CCCTGTCCTA 
GCAACTAGTT CAGGAGCTGG TGTTTTTAAG ATGGGACGAT TTCAGGTTTC TGTTGCAGCA 
GACGGTGCCC AGAAAGAGGG TAAAAATAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACCT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CTTGGTGAAA 
CCAGAGCCGA ATGGCATAAC CATCCCTGGT ATCTCTTCAG ATGTGCCAGA GAGTGCCCAC 
AAAACTACTG CCTCAGAGGC AAAGTCAGAC ACTGGGCAGC CTACCAAGGT TGGACGTTTT 
CAGGTGACAA CTACAGCAAA CAAAGTGGGT CGTTTCTCTG TATCAAAAAC TGAGGACAAG 
ATCACTGACA CAAAGAAAGA AGGACCAGTG GCATCTCCTC CTTTTATGGA TTTGGAACAA 
GCTGTTCTTC CTGCTGTGAT ACCAAAGAAA GAGAAGCCTG AACTGTCAGA GCCTTCACAT 
CTAAATGGGC CGTCTTCTGA CCCGGAGGCC GCTTTTTTAA GTAGGGATGT GGATGATGGT 
TCCGGTAGTC CACACTCGCC CCATCAGCTG AGCTCAAAGA GCCTTCCTAG CCAGAATCTA 
AGTCAAAGCC TTAGTAATTC ATTTAACTCC TCTTACATGA GTAGCGACAA TGAGTCAGAT 
ATCGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGATAAACA TCTCAAAGAG 
ATTCAGGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC CAAACTGGGC 
AAGGTGCCCC CTGCTGTTAT TATTCCCCCA GCTGCTCCCC TTTCAGGGAG AAGACGACGA 
CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGAAGCAGTT CCTTGGGGAA TAAAAGCCCC 
CAGCTTTCAG GTAACCTGTC TGGTCAGAGT GCAGCTTCAG TCTTGCACCC CCAGCAGACC 
CTCCACCCTC CTGGCAACAT CCCAGAGTCC GGGCAGAATC AGCTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTCTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG TCAAGGAACC AGCAGCACAA ACACTGTTGG GGCAACAGTG 
AACAGCCAAG CCGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACATTC 
ACAGATGACT TGCACAAGTT GGTAGACAAT TGGGCCCGAG ATGCCATGAA TCTCTCAGGC 
AGGAGAGGAA GCAAAGGGCA CATGAATTAC GAGGGCCCTG GAATGGCAAG GAAGTTCTCT 
GCACCTGGGC AACTGTGCAT CTCCATGACC TCGAACCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAT 
GGCTTTCCAG CTACCCCATT TGGCGCTCAA TGGAGTGGGA CGGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACT GCCTCCTTGC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGC TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875326.1
CDS	969..7379
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_017019837.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019837.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAGCC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGAGTACTC AGGGAGTCTC TCAGGTTGCT 
CCTGCAGAGC CAGTTGCAGT AGCACAGACC CAAGCTACCC AGCCGACCAC TTTGGCTTCC 
TCTGTAGACA GTGCACATTC AGATGTTGCT TCAGGTATGA GTGATGGCAA TGAGAACGTC 
CCATCTTCCA GTGGAAGGCA TGAAGGAAGA ACTACAAAAC GGCATTACCG AAAATCTGTA 
AGGAGTCGCT CTCGACATGA AAAAACTTCA CGCCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA TTAGAGACTC ATAATAGGAA AATGGTTACA 
TTCAAATTTG ACCTAGATGG TGACAACCCC GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATAGA GAGAGAGTCG TTTGTGGATC AAGTGCGAGA AATTATTGAA 
AAAGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA TCAGGGATTG 
GAGAGTCTAC AAGGAAAGGA TGACTATGGC TTTTCAGGTT CTCAGAAATT GGAAGGAGAG 
TTCAAACAAC CAATTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCGGGAAGG CGGTTTATAG TGAGTCCTGT GCCAGAAAGC 
CGATTACGAG AATCAAAAGT TTTCCCCAGT GAAATAACAG ATACAGTTGC TGCCTCTACA 
GCTCAGAGCC CTGGAATGAA CTTGTCTCAC TCTGCATCAT CCCTTAGTCT ACAACAGGCC 
TTTTCTGAAC TTAGACGTGC CCAAATGACA GAAGGACCCA ACACAGCACC TCCAAACTTT 
AGTCATACAG GACCAACATT TCCAGTAGTA CCTCCTTTCT TAAGTAGCAT TGCTGGAGTC 
CCAACCACAG CAGCAGCCAC AGCACCAGTC CCTGCAACAA GCAGCCCTCC TAATGACATT 
TCCACATCAG TAATTCAGTC TGAGGTTACA GTGCCCACTG AAGAGGGGAT TGCTGGAGTT 
GCCACCAGCA CAGGTGTGGT AACTTCAGGT GGTCTCCCCA TACCACCTGT GTCTGAATCA 
CCAGTACTTT CCAGCGTAGT TTCAAGTATC ACAATACCTG CAGTTGTCTC AATATCTACT 
ACATCCCCGT CACTTCAAGT CCCCACATCC ACATCTGAGA TCGTTGTTTC TAGTACAGCA 
CTGTATCCTT CAGTAACAGT TTCAGCAACT TCAGCCTCTG CAGGGGGCAG TACTGCTACC 
CCAGGTCCTA AGCCTCCAGC TGTAGTATCT CAGCAGGCAG CAGGCAGCAC TACTGTGGGA 
GCCACATTAA CATCAGTTTC TACCACCACT TCATTCCCAA GCACAGCTTC ACAGCTGTGC 
ATTCAGCTTA GCAGCAGTAC TTCTACTCCT ACTTTAGCTG AAACCGTGGT AGTTAGCGCA 
CACTCACTAG ATAAGACATC TCATAGCAGT ACAACTGGAT TGGCTTTCTC CCTCTCTGCA 
CCATCTTCCT CTTCCTCTCC TGGAGCAGGA GTGTCTAGTT ATATTTCTCA GCCTGGTGGG 
CTGCATCCTT TGGTCATTCC ATCAGTGATA GCTTCTACTC CTATTCTTCC CCAAGCAGCA 
GGACCTACTT CTACACCTTT ATTACCCCAA GTACCTAGTA TCCCACCCTT GGTACAGCCT 
GTTGCCAATG TGCCTGCTGT ACAGCAGACA CTAATTCATA GTCAGCCTCA ACCAGCTTTG 
CTTCCCAACC AGCCCCATAC TCATTGTCCT GAAGTAGATT CTGATACACA ACCCAAAGCT 
CCTGGAATTG ATGACATAAA GACTCTAGAA GAAAAGCTGC GGTCTCTGTT CAGTGAACAC 
AGCTCATCTG GAGCTCAGCA TGCCTCTGTC TCACTGGAGA CCTCACTAGT CATAGAGAGC 
ACTGTCACAC CAGGCATCCC AACTACTGCT GTTGCACCAA GCAAACTCCT GACTTCTACC 
ACAAGTACTT GCTTACCACC AACCAATTTA CCACTAGGAA CAGTTGCTTT GCCAGTTACA 
CCAGTGGTCA CACCTGGGCA AGTTTCTACC CCAGTCAGCA CTACTACATC AGGAGTGAAA 
CCTGGAACTG CTCCCTCCAA GCCACCTCTA ACTAAGGCTC CGGTGCTGCC AGTGGGTACT 
GAACTTCCAG CAGGTACTCT ACCCAGCGAG CAGCTGCCAC CTTTTCCAGG ACCTTCTCTA 
ACCCAGTCCC AGCAACCTCT AGAGGATCTT GATGCTCAAT TGAGAAGAAC ACTTAGTCCA 
GAGATGATCA CAGTGACTTC TGCGGTTGGT CCTGTGTCCA TGGCGGCTCC AACAGCAATC 
ACAGAAGCAG GAACACAGCC TCAGAAGGGT GTTTCTCAAG TCAAAGAAGG CCCTGTCCTA 
GCAACTAGTT CAGGAGCTGG TGTTTTTAAG ATGGGACGAT TTCAGGTTTC TGTTGCAGCA 
GACGGTGCCC AGAAAGAGGG TAAAAATAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACCT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CTTGGTGAAA 
CCAGAGCCGA ATGGCATAAC CATCCCTGGT ATCTCTTCAG ATGTGCCAGA GAGTGCCCAC 
AAAACTACTG CCTCAGAGGC AAAGTCAGAC ACTGGGCAGC CTACCAAGGT TGGACGTTTT 
CAGGTGACAA CTACAGCAAA CAAAGTGGGT CGTTTCTCTG TATCAAAAAC TGAGGACAAG 
ATCACTGACA CAAAGAAAGA AGGACCAGTG GCATCTCCTC CTTTTATGGA TTTGGAACAA 
GCTGTTCTTC CTGCTGTGAT ACCAAAGAAA GAGAAGCCTG AACTGTCAGA GCCTTCACAT 
CTAAATGGGC CGTCTTCTGA CCCGGAGGCC GCTTTTTTAA GTAGGGATGT GGATGATGGT 
TCCGGTAGTC CACACTCGCC CCATCAGCTG AGCTCAAAGA GCCTTCCTAG CCAGAATCTA 
AGTCAAAGCC TTAGTAATTC ATTTAACTCC TCTTACATGA GTAGCGACAA TGAGTCAGAT 
ATCGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGATAAACA TCTCAAAGAG 
ATTCAGGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC CAAACTGGGC 
AAGGTGCCCC CTGCTGTTAT TATTCCCCCA GCTGCTCCCC TTTCAGGGAG AAGACGACGA 
CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGAAGCAGTT CCTTGGGGAA TAAAAGCCCC 
CAGCTTTCAG GTAACCTGTC TGGTCAGAGT GCAGCTTCAG TCTTGCACCC CCAGCAGACC 
CTCCACCCTC CTGGCAACAT CCCAGAGTCC GGGCAGAATC AGCTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTCTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG TCAAGGAACC AGCAGCACAA ACACTGTTGG GGCAACAGTG 
AACAGCCAAG CCGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACATTC 
ACAGATGACT TGCACAAGTT GGTAGACAAT TGGGCCCGAG ATGCCATGAA TCTCTCAGGC 
AGGAGAGGAA GCAAAGGGCA CATGAATTAC GAGGGCCCTG GAATGGCAAG GAAGTTCTCT 
GCACCTGGGC AACTGTGCAT CTCCATGACC TCGAACCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAT 
GGCTTTCCAG CTACCCCATT TGGCGCTCAA TGGAGTGGGA CGGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACT GCCTCCTTGC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGC TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_017019838.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875327.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.

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CloneID	OHu84078
Clone ID Related Accession (Same CDS sequence)	XM_017019838.1
Accession Version	XM_017019838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT 
GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT 
GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA 
GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA 
ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC 
GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA 
GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA 
ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG 
GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC 
ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT 
GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA 
AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC 
GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT 
CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT 
CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG 
GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC 
ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG 
CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC 
CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA 
TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGTCA AGGAACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC 
AGCCAAGCCG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACA 
GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG 
AGAGGAAGCA AAGGGCACAT GAATTACGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA 
CCTGGGCAAC TGTGCATCTC CATGACCTCG AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC 
TTTCCAGCTA CCCCATTTGG CGCTCAATGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875327.1
CDS	969..7376
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_017019838.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019838.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT 
GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT 
GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA 
GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA 
ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC 
GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA 
GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA 
ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG 
GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC 
ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT 
GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA 
AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC 
GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT 
CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT 
CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG 
GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC 
ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG 
CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC 
CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA 
TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGTCA AGGAACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC 
AGCCAAGCCG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACA 
GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG 
AGAGGAAGCA AAGGGCACAT GAATTACGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA 
CCTGGGCAAC TGTGCATCTC CATGACCTCG AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC 
TTTCCAGCTA CCCCATTTGG CGCTCAATGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_017019839.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875328.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA.

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CloneID	OHu84079
Clone ID Related Accession (Same CDS sequence)	XM_017019839.1
Accession Version	XM_017019839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA 
CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA 
GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA 
ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA 
TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC 
AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA 
GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC 
TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT 
TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG 
GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC 
CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG 
TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA 
ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA 
GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA 
CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG 
GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT 
TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA 
AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC 
ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA 
GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA 
GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC 
ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT 
ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA 
GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA 
GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA 
CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT 
TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC 
TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT 
ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC 
AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT 
TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA 
CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC 
AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG 
GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG 
GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG 
AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT 
TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA 
AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG 
CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC 
CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG 
TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA 
GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA 
CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA 
AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG 
GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT 
CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT 
AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA 
GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG 
AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT 
CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA 
TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGAAC CAGCAGCACA 
AACACTGTTG GGGCAACAGT GAACAGCCAA GCCGCCCAAG CTCAGCCTCC TGCCATGACG 
TCCAGCAGGA AGGGCACATT CACAGATGAC TTGCACAAGT TGGTAGACAA TTGGGCCCGA 
GATGCCATGA ATCTCTCAGG CAGGAGAGGA AGCAAAGGGC ACATGAATTA CGAGGGCCCT 
GGAATGGCAA GGAAGTTCTC TGCACCTGGG CAACTGTGCA TCTCCATGAC CTCGAACCTG 
GGTGGCTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA TGGCTTTCCA GCTACCCCAT TTGGCGCTCA ATGGAGTGGG 
ACGGGTGGCC CAGCACCACA GCCACTTGGC CAGTTCCAAC CTGTGGGAAC TGCCTCCTTG 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG CTCCAACCTG 
CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875328.1
CDS	969..7100
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_017019839.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019839.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA 
CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA 
GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA 
ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA 
TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC 
AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA 
GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC 
TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT 
TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG 
GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC 
CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG 
TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA 
ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA 
GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA 
CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG 
GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT 
TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA 
AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC 
ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA 
GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA 
GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC 
ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT 
ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA 
GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA 
GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA 
CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT 
TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC 
TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT 
ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC 
AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT 
TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA 
CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC 
AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG 
GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG 
GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG 
AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT 
TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA 
AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG 
CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC 
CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG 
TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA 
GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA 
CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA 
AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG 
GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT 
CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT 
AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA 
GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG 
AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT 
CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA 
TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGAAC CAGCAGCACA 
AACACTGTTG GGGCAACAGT GAACAGCCAA GCCGCCCAAG CTCAGCCTCC TGCCATGACG 
TCCAGCAGGA AGGGCACATT CACAGATGAC TTGCACAAGT TGGTAGACAA TTGGGCCCGA 
GATGCCATGA ATCTCTCAGG CAGGAGAGGA AGCAAAGGGC ACATGAATTA CGAGGGCCCT 
GGAATGGCAA GGAAGTTCTC TGCACCTGGG CAACTGTGCA TCTCCATGAC CTCGAACCTG 
GGTGGCTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA TGGCTTTCCA GCTACCCCAT TTGGCGCTCA ATGGAGTGGG 
ACGGGTGGCC CAGCACCACA GCCACTTGGC CAGTTCCAAC CTGTGGGAAC TGCCTCCTTG 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG CTCCAACCTG 
CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( NM_213655.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_998820.3 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 3, mRNA.

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CloneID	OHu28095
Clone ID Related Accession (Same CDS sequence)	NM_213655.4
Accession Version	NM_213655.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004765.2 and AC004803.11. This sequence is a reference standard in the RefSeqGene project. On Jul 17, 2010 this sequence version replaced NM_213655.3. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame compared to variant 1. This variant represents the exon combination of the brain and spinal cord variant described in Figure 2F of PubMed ID 18521183. This variant encodes isoform 3, which is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. The combination of alternatively spliced exons within the coding region is inferred based on experimental evidence reported in Figures 2F and 3 from PubMed ID 18521183. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2156670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCGTCGTGG CCGTAGCATG 
TCGGTTTGTG TTCCCATCTT TCTGCTGTTG CCTCTGTGTC CCGCATCTCT CCCAGTGCTC 
TTCCACCCCA CCGCCAGTAC TGTCTGCACC TCTTTCTCCT TCCCTCCTCC GGACTGCCCC 
GAGGAAACTT TTGCCGAAAA GCTTTCTAAA GCATTGGAGA GTGTCCTGCC TATGCACTCT 
GCCTCTCAGC GCAAGCACCG ACGCTCCAGC CTGCCTTCCC TCTTTGTCAG TACTCCTCAG 
TCCATGGCGC ATCCGTGTGG GGGGACCCCA ACATACCCAG AATCACAGAT ATTTTTCCCA 
ACTATTCATG AACGTCCAGT TTCTTTTTCA CCACCTCCCA CCTGCCCACC GAAAGTAGCC 
ATTTCCCAGC GGCGTAAGAG CACCTCCTTC CTGGAAGCCC AAACTCACCA CTTCCAACCC 
CTGCTGAGGA CTGTTGGCCA AAGTCTTCTT CCACCTGGTG GCAGCCCAAC TAACTGGACA 
CCAGAGGCCG TAGTTATGTT GGGTACTACA GCCAGTAGAG TAACTGGAGA GTCATGTGAG 
ATACAGGTCC ATCCTATGTT TGAACCATCT CAAGTTTACA GTGACTATAG ACCTGGACTA 
GTACTTCCAG AAGAAGCTCA CTATTTTATT CCTCAGGAAG CAGTGTATGT AGCTGGGGTA 
CATTACCAGG CCCGGGTGGC AGAACAGTAT GAGGGCATTC CATACAACTC ATCAGTACTG 
TCAAGTCCTA TGAAACAGAT ACCTGAACAG AAGCCAGTAC AAGGGGGCCC TACTTCAAGT 
TCTGTCTTTG AATTTCCATC TGGACAGGCT TTCCTGGTAG GACACCTTCA GAATCTAAGA 
TTAGATTCTG GATTGGGTCC GGGATCTCCC CTCTCTAGTA TTTCTGCACC TATCAGTACA 
GATGCTACAC GTTTGAAATT TCACCCTGTC TTTGTTCCTC ATTCTGCGCC TGCTGTGTTA 
ACTCATAACA ATGAGAGCAG AAGCAACTGT GTATTTGAAT TTCATGTTCA CACACCAAGC 
TCCTCTTCAG GAGAAGGAGG TGGAATTTTA CCTCAGCGTG TTTACCGAAA TCGGCAGGTT 
GCAGTGGACT TGAATCAAGA AGAACTGCCT CCTCAATCAG TTGGATTACA TGGCTACTTG 
CAGCCTGTGA CTGAAGAAAA GCATAATTAC CATGCCCCAG AATTGACCGT TTCTGTGGTA 
GAGCCTATCG GACAGAACTG GCCAATAGGA AGCCCAGAAT ATTCCAGTGA TTCCTCACAA 
ATCACTTCTT CAGACCCCAG TGATTTTCAG TCACCTCCCC CTACAGGGGG AGCAGCTGCA 
CCTTTTGGCT CTGACGTCTC AATGCCCTTT ATCCATCTGC CTCAGACAGT GTTACAAGAA 
TCCCCACTTT TCTTCTGTTT CCCCCAAGGA ACCACATCTC AGCAGGTCTT AACTGCCTCA 
TTTTCTTCAG GAGGATCTGC ACTTCATCCA CAGGCACAGG GGCAGAGCCA GGGTCAGCCA 
TCCTCAAGTA GCTTAACAGG GGTTTCATCT TCCCAACCCA TACAACATCC TCAGCAGCAG 
CAGGGAATAC AGCAGACAGC CCCTCCTCAA CAGACAGTGC AGTATTCACT TTCACAGACA 
TCAACCTCCA GTGAGGCCAC TACTGCACAG CCAGTGAGTC AGCCTCAAGC TCCACAAGTC 
TTGCCTCAAG TATCAGCTGG AAAACAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTGCA 
GAGCCAGTTG CAGTAGCACA GACCCAAGCT ACCCAGCCGA CCACTTTGGC TTCCTCTGTA 
GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA CGTCCCATCT 
TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT 
CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA 
GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA 
TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT 
ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGATCAGGG ATTGGAGAGT 
CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA 
CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT 
CAAGTTGTTC ATTCTGCGGG AAGGCGGTTT ATAGTGAGTC CTGTGCCAGA AAGCCGATTA 
CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACAGCTCAG 
AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT 
GAACTTAGAC GTGCCCAAAT GACAGAAGGA CCCAACACAG CACCTCCAAA CTTTAGTCAT 
ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC 
ACAGCAGCAG CCACAGCACC AGTCCCTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA 
TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC 
AGCACAGGTG TGGTAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA 
CTTTCCAGCG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC 
CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGATCGTTG TTTCTAGTAC AGCACTGTAT 
CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT 
CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA 
TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTGCATTCAG 
CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA 
CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT 
TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTATATTT CTCAGCCTGG TGGGCTGCAT 
CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT 
ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA GCCTGTTGCC 
AATGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC 
AACCAGCCCC ATACTCATTG TCCTGAAGTA GATTCTGATA CACAACCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TGTTCAGTGA ACACAGCTCA 
TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC 
ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TACCACAAGT 
ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG 
GTCACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGAGT GAAACCTGGA 
ACTGCTCCCT CCAAGCCACC TCTAACTAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT 
CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG 
TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATG 
ATCACAGTGA CTTCTGCGGT TGGTCCTGTG TCCATGGCGG CTCCAACAGC AATCACAGAA 
GCAGGAACAC AGCCTCAGAA GGGTGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT 
AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998820.3
CDS	644..8548
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVPRRGRSMSVCVPIFLLLPLCPASLPVLFHPTASTVCTSFSFPPPDCPEETFAEKLSKALESVLPMHSASQRKHRRSSLPSLFVSTPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	NM_213655.4
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213655.4

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCGTCGTGG CCGTAGCATG 
TCGGTTTGTG TTCCCATCTT TCTGCTGTTG CCTCTGTGTC CCGCATCTCT CCCAGTGCTC 
TTCCACCCCA CCGCCAGTAC TGTCTGCACC TCTTTCTCCT TCCCTCCTCC GGACTGCCCC 
GAGGAAACTT TTGCCGAAAA GCTTTCTAAA GCATTGGAGA GTGTCCTGCC TATGCACTCT 
GCCTCTCAGC GCAAGCACCG ACGCTCCAGC CTGCCTTCCC TCTTTGTCAG TACTCCTCAG 
TCCATGGCGC ATCCGTGTGG GGGGACCCCA ACATACCCAG AATCACAGAT ATTTTTCCCA 
ACTATTCATG AACGTCCAGT TTCTTTTTCA CCACCTCCCA CCTGCCCACC GAAAGTAGCC 
ATTTCCCAGC GGCGTAAGAG CACCTCCTTC CTGGAAGCCC AAACTCACCA CTTCCAACCC 
CTGCTGAGGA CTGTTGGCCA AAGTCTTCTT CCACCTGGTG GCAGCCCAAC TAACTGGACA 
CCAGAGGCCG TAGTTATGTT GGGTACTACA GCCAGTAGAG TAACTGGAGA GTCATGTGAG 
ATACAGGTCC ATCCTATGTT TGAACCATCT CAAGTTTACA GTGACTATAG ACCTGGACTA 
GTACTTCCAG AAGAAGCTCA CTATTTTATT CCTCAGGAAG CAGTGTATGT AGCTGGGGTA 
CATTACCAGG CCCGGGTGGC AGAACAGTAT GAGGGCATTC CATACAACTC ATCAGTACTG 
TCAAGTCCTA TGAAACAGAT ACCTGAACAG AAGCCAGTAC AAGGGGGCCC TACTTCAAGT 
TCTGTCTTTG AATTTCCATC TGGACAGGCT TTCCTGGTAG GACACCTTCA GAATCTAAGA 
TTAGATTCTG GATTGGGTCC GGGATCTCCC CTCTCTAGTA TTTCTGCACC TATCAGTACA 
GATGCTACAC GTTTGAAATT TCACCCTGTC TTTGTTCCTC ATTCTGCGCC TGCTGTGTTA 
ACTCATAACA ATGAGAGCAG AAGCAACTGT GTATTTGAAT TTCATGTTCA CACACCAAGC 
TCCTCTTCAG GAGAAGGAGG TGGAATTTTA CCTCAGCGTG TTTACCGAAA TCGGCAGGTT 
GCAGTGGACT TGAATCAAGA AGAACTGCCT CCTCAATCAG TTGGATTACA TGGCTACTTG 
CAGCCTGTGA CTGAAGAAAA GCATAATTAC CATGCCCCAG AATTGACCGT TTCTGTGGTA 
GAGCCTATCG GACAGAACTG GCCAATAGGA AGCCCAGAAT ATTCCAGTGA TTCCTCACAA 
ATCACTTCTT CAGACCCCAG TGATTTTCAG TCACCTCCCC CTACAGGGGG AGCAGCTGCA 
CCTTTTGGCT CTGACGTCTC AATGCCCTTT ATCCATCTGC CTCAGACAGT GTTACAAGAA 
TCCCCACTTT TCTTCTGTTT CCCCCAAGGA ACCACATCTC AGCAGGTCTT AACTGCCTCA 
TTTTCTTCAG GAGGATCTGC ACTTCATCCA CAGGCACAGG GGCAGAGCCA GGGTCAGCCA 
TCCTCAAGTA GCTTAACAGG GGTTTCATCT TCCCAACCCA TACAACATCC TCAGCAGCAG 
CAGGGAATAC AGCAGACAGC CCCTCCTCAA CAGACAGTGC AGTATTCACT TTCACAGACA 
TCAACCTCCA GTGAGGCCAC TACTGCACAG CCAGTGAGTC AGCCTCAAGC TCCACAAGTC 
TTGCCTCAAG TATCAGCTGG AAAACAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTGCA 
GAGCCAGTTG CAGTAGCACA GACCCAAGCT ACCCAGCCGA CCACTTTGGC TTCCTCTGTA 
GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA CGTCCCATCT 
TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT 
CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA 
GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA 
TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT 
ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGATCAGGG ATTGGAGAGT 
CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA 
CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT 
CAAGTTGTTC ATTCTGCGGG AAGGCGGTTT ATAGTGAGTC CTGTGCCAGA AAGCCGATTA 
CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACAGCTCAG 
AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT 
GAACTTAGAC GTGCCCAAAT GACAGAAGGA CCCAACACAG CACCTCCAAA CTTTAGTCAT 
ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC 
ACAGCAGCAG CCACAGCACC AGTCCCTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA 
TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC 
AGCACAGGTG TGGTAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA 
CTTTCCAGCG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC 
CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGATCGTTG TTTCTAGTAC AGCACTGTAT 
CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT 
CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA 
TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTGCATTCAG 
CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA 
CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT 
TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTATATTT CTCAGCCTGG TGGGCTGCAT 
CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT 
ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA GCCTGTTGCC 
AATGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC 
AACCAGCCCC ATACTCATTG TCCTGAAGTA GATTCTGATA CACAACCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TGTTCAGTGA ACACAGCTCA 
TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC 
ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TACCACAAGT 
ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG 
GTCACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGAGT GAAACCTGGA 
ACTGCTCCCT CCAAGCCACC TCTAACTAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT 
CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG 
TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATG 
ATCACAGTGA CTTCTGCGGT TGGTCCTGTG TCCATGGCGG CTCCAACAGC AATCACAGAA 
GCAGGAACAC AGCCTCAGAA GGGTGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT 
AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( NM_001184985.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171914.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 4, mRNA.

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CloneID	OHu28140
Clone ID Related Accession (Same CDS sequence)	NM_001184985.1
Accession Version	NM_001184985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004765.2 and AC004803.11. Transcript Variant: This variant (4) has multiple differences in the coding region but maintains the reading frame compared to variant 1. This variant represents the exon combination of the dorsal root ganglia and sciatic nerve variant described in Figure 2F of PubMed ID 18521183. This variant encodes isoform 4, which is longer than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. The combination of alternatively spliced exons within the coding region is inferred based on experimental evidence reported in Figures 2F and 3 from PubMed ID 18521183. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCAACGTGGC TTCTGTTTGC ATCCATTCTA CAGTCCTATC CCCTCCCATG 
CCGACAGAAG TACTGGCTAC ACCTGGGTAC TTTCCCACAG TGGTGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTAT GGGTGGTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GGGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG 
CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC 
GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT 
ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG 
GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC 
CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC 
TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG 
CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT 
GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC 
ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA 
ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171914.1
CDS	644..8572
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	NM_001184985.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184985.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCAACGTGGC TTCTGTTTGC ATCCATTCTA CAGTCCTATC CCCTCCCATG 
CCGACAGAAG TACTGGCTAC ACCTGGGTAC TTTCCCACAG TGGTGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTAT GGGTGGTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GGGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG 
CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC 
GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT 
ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG 
GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC 
CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC 
TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG 
CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT 
GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC 
ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA 
ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( NM_014823.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055638.2 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 2, mRNA.

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CloneID	OHu28145
Clone ID Related Accession (Same CDS sequence)	NM_014823.2 , NM_014823.3
Accession Version	NM_014823.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004765.2, AJ296290.1, CD629562.1, AB002342.2, AC004803.11, BX644268.1, DA720959.1, BC141881.1 and AI074334.1. On May 27, 2010 this sequence version replaced NM_014823.1. Transcript Variant: This variant (2) uses two alternative splice sites and lacks two exons in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB002342.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT 
GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT 
GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA 
GAAGCAGGAA CACAGCCTCA GAAGGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT 
AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055638.2
CDS	644..7048
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	NM_014823.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014823.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT 
GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT 
GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA 
GAAGCAGGAA CACAGCCTCA GAAGGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT 
AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( NM_014823.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055638.2 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 2, mRNA.

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CloneID	OHu28145
Clone ID Related Accession (Same CDS sequence)	NM_014823.2 , NM_014823.3
Accession Version	NM_014823.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004765.2, AJ296290.1, CD629562.1, AB002342.2, AC004803.11, BX644268.1, DA720959.1, BC141881.1 and AI074334.1. On May 31, 2019 this sequence version replaced NM_014823.2. Transcript Variant: This variant (2) uses two alternative splice sites and lacks two exons in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB002342.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT 
GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT 
GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA 
GAAGCAGGAA CACAGCCTCA GAAGGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT 
AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055638.2
CDS	988..7392
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	NM_014823.3
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014823.3

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT 
GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT 
GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA 
GAAGCAGGAA CACAGCCTCA GAAGGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT 
AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( NM_018979.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_061852.3 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 1, mRNA.

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CloneID	OHu28188
Clone ID Related Accession (Same CDS sequence)	NM_018979.3 , NM_018979.4
Accession Version	NM_018979.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004765.2, AJ296290.1, CD629562.1, AB002342.2, BX644268.1, DA720959.1, AC004803.11, BC141881.1 and AI074334.1. This sequence is a reference standard in the RefSeqGene project. On May 27, 2010 this sequence version replaced NM_018979.2. Transcript Variant: This variant (1) encodes the most common isoform (1), as indicated in PubMed ID 18521183. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ296290.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAGCC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTC CCTTGCTCCC TCAGTACCCT 
GTCTCTCAGA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCAACGTGGC TTCTGTTTGC ATCCATTCTA CAGTCCTATC CCCTCCCATG 
CCGACAGAAG TACTGGCTAC ACCTGGGTAC TTTCCCACAG TGGTGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTAT GGGTGGTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GGGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG 
CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC 
GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT 
ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG 
GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC 
CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC 
TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG 
CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT 
GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC 
ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA 
ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061852.3
CDS	644..7792
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	NM_018979.3
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018979.3

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAGCC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTC CCTTGCTCCC TCAGTACCCT 
GTCTCTCAGA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCAACGTGGC TTCTGTTTGC ATCCATTCTA CAGTCCTATC CCCTCCCATG 
CCGACAGAAG TACTGGCTAC ACCTGGGTAC TTTCCCACAG TGGTGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTAT GGGTGGTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GGGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG 
CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC 
GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT 
ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG 
GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC 
CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC 
TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG 
CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT 
GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC 
ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA 
ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( NM_018979.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_061852.3 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 1, mRNA.

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CloneID	OHu28188
Clone ID Related Accession (Same CDS sequence)	NM_018979.3 , NM_018979.4
Accession Version	NM_018979.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004765.2, AJ296290.1, CD629562.1, AB002342.2, BX644268.1, DA720959.1, AC004803.11, BC141881.1 and AI074334.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_018979.3. Transcript Variant: This variant (1) encodes the most common isoform (1), as indicated in PubMed ID 18521183. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ296290.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000315939.11/ ENSP00000313059.6 RefSeq Select criteria :: based on expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAGCC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTC CCTTGCTCCC TCAGTACCCT 
GTCTCTCAGA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCAACGTGGC TTCTGTTTGC ATCCATTCTA CAGTCCTATC CCCTCCCATG 
CCGACAGAAG TACTGGCTAC ACCTGGGTAC TTTCCCACAG TGGTGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTAT GGGTGGTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GGGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG 
CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC 
GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT 
ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG 
GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC 
CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC 
TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG 
CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT 
GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC 
ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA 
ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061852.3
CDS	988..8136
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	NM_018979.4
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018979.4

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAGCC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTC CCTTGCTCCC TCAGTACCCT 
GTCTCTCAGA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCAACGTGGC TTCTGTTTGC ATCCATTCTA CAGTCCTATC CCCTCCCATG 
CCGACAGAAG TACTGGCTAC ACCTGGGTAC TTTCCCACAG TGGTGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTAT GGGTGGTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GGGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG 
CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC 
GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT 
ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG 
GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC 
CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC 
TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG 
CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT 
GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC 
ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA 
ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_006719003.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719066.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

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CloneID	OHu46009
Clone ID Related Accession (Same CDS sequence)	XM_006719003.2
Accession Version	XM_006719003.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG CTTCCAGTTT CCCAGCCAGT ACCAACTATC 
CAAGGCGAAC CTCAGATCCC AGTTGCGACA CAACCCTCGG TTGTTCCAGT CCACTCTGGT 
GCTCATTTCC TTCCAGTGGG ACAGCCGCTC CCTACTCCCT TGCTCCCTCA GTACCCTGTC 
TCTCAGATTC CCATATCAAC TCCTCATGTG TCTACGGCTC AGACAGGTTT CTCATCCCTT 
CCCATCACAA TGGCAGCTGG CATTACTCAG CCTCTGCTCA CGTTGGCTTC ATCTGCTACA 
ACAGCTGCGA TCCCGGGGGT ATCAACTGTG GTTCCTAGTC AGCTTCCAAC CCTTCTGCAG 
CCTGTGACTC AGCTGCCAAG TCAGGTTCAC CCACAGCTCC TACAACCAGC AGTTCAGTCC 
ATGGGAATAC CAGCTAACCT TGGACAAGCT GCTGAGGTTC CACTTTCCTC TGGAGATGTT 
CTGTACCAGG GCTTCCCACC TCGACTGCCA CCACAGTACC CAGGAGATTC AAATATTGCT 
CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTATCCCC TCCCATGCCG 
ACAGAAGTAC TGGCTACACC TGGGTACTTT CCCACAGTGG TGCAGCCTTA TGTGGAATCA 
AATCTTTTAG TTCCTATGGG TGGTGTAGGA GGACAGGTTC AAGTGTCCCA GCCAGGAGGG 
AGTTTAGCAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG TTTTGGAGAG TACTCAGGGA 
GTCTCTCAGG TTGCTCCTGC AGAGCCAGTT GCAGTAGCAC AGACCCAAGC TACCCAGCCG 
ACCACTTTGG CTTCCTCTGT AGACAGTGCA CATTCAGATG TTGCTTCAGG TATGAGTGAT 
GGCAATGAGA ACGTCCCATC TTCCAGTGGA AGGCATGAAG GAAGAACTAC AAAACGGCAT 
TACCGAAAAT CTGTAAGGAG TCGCTCTCGA CATGAAAAAA CTTCACGCCC AAAATTAAGA 
ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAATTAGA GACTCATAAT 
AGGAAAATGG TTACATTCAA ATTTGACCTA GATGGTGACA ACCCCGAGGA GATAGCAACA 
ATTATGGTGA ACAATGACTT TATTCTAGCA ATAGAGAGAG AGTCGTTTGT GGATCAAGTG 
CGAGAAATTA TTGAAAAAGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG 
GGTGATCAGG GATTGGAGAG TCTACAAGGA AAGGATGACT ATGGCTTTTC AGGTTCTCAG 
AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCGTCTT CCATGCCACA GCAAATAGGC 
ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCGG GAAGGCGGTT TATAGTGAGT 
CCTGTGCCAG AAAGCCGATT ACGAGAATCA AAAGTTTTCC CCAGTGAAAT AACAGATACA 
GTTGCTGCCT CTACAGCTCA GAGCCCTGGA ATGAACTTGT CTCACTCTGC ATCATCCCTT 
AGTCTACAAC AGGCCTTTTC TGAACTTAGA CGTGCCCAAA TGACAGAAGG ACCCAACACA 
GCACCTCCAA ACTTTAGTCA TACAGGACCA ACATTTCCAG TAGTACCTCC TTTCTTAAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGCA GCCACAGCAC CAGTCCCTGC AACAAGCAGC 
CCTCCTAATG ACATTTCCAC ATCAGTAATT CAGTCTGAGG TTACAGTGCC CACTGAAGAG 
GGGATTGCTG GAGTTGCCAC CAGCACAGGT GTGGTAACTT CAGGTGGTCT CCCCATACCA 
CCTGTGTCTG AATCACCAGT ACTTTCCAGC GTAGTTTCAA GTATCACAAT ACCTGCAGTT 
GTCTCAATAT CTACTACATC CCCGTCACTT CAAGTCCCCA CATCCACATC TGAGATCGTT 
GTTTCTAGTA CAGCACTGTA TCCTTCAGTA ACAGTTTCAG CAACTTCAGC CTCTGCAGGG 
GGCAGTACTG CTACCCCAGG TCCTAAGCCT CCAGCTGTAG TATCTCAGCA GGCAGCAGGC 
AGCACTACTG TGGGAGCCAC ATTAACATCA GTTTCTACCA CCACTTCATT CCCAAGCACA 
GCTTCACAGC TGTGCATTCA GCTTAGCAGC AGTACTTCTA CTCCTACTTT AGCTGAAACC 
GTGGTAGTTA GCGCACACTC ACTAGATAAG ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTCTCCCTCT CTGCACCATC TTCCTCTTCC TCTCCTGGAG CAGGAGTGTC TAGTTATATT 
TCTCAGCCTG GTGGGCTGCA TCCTTTGGTC ATTCCATCAG TGATAGCTTC TACTCCTATT 
CTTCCCCAAG CAGCAGGACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAGTATCCCA 
CCCTTGGTAC AGCCTGTTGC CAATGTGCCT GCTGTACAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTGCTTCC CAACCAGCCC CATACTCATT GTCCTGAAGT AGATTCTGAT 
ACACAACCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAAGAAAA GCTGCGGTCT 
CTGTTCAGTG AACACAGCTC ATCTGGAGCT CAGCATGCCT CTGTCTCACT GGAGACCTCA 
CTAGTCATAG AGAGCACTGT CACACCAGGC ATCCCAACTA CTGCTGTTGC ACCAAGCAAA 
CTCCTGACTT CTACCACAAG TACTTGCTTA CCACCAACCA ATTTACCACT AGGAACAGTT 
GCTTTGCCAG TTACACCAGT GGTCACACCT GGGCAAGTTT CTACCCCAGT CAGCACTACT 
ACATCAGGAG TGAAACCTGG AACTGCTCCC TCCAAGCCAC CTCTAACTAA GGCTCCGGTG 
CTGCCAGTGG GTACTGAACT TCCAGCAGGT ACTCTACCCA GCGAGCAGCT GCCACCTTTT 
CCAGGACCTT CTCTAACCCA GTCCCAGCAA CCTCTAGAGG ATCTTGATGC TCAATTGAGA 
AGAACACTTA GTCCAGAGAT GATCACAGTG ACTTCTGCGG TTGGTCCTGT GTCCATGGCG 
GCTCCAACAG CAATCACAGA AGCAGGAACA CAGCCTCAGA AGGGTGTTTC TCAAGTCAAA 
GAAGGCCCTG TCCTAGCAAC TAGTTCAGGA GCTGGTGTTT TTAAGATGGG ACGATTTCAG 
GTTTCTGTTG CAGCAGACGG TGCCCAGAAA GAGGGTAAAA ATAAGTCAGA AGATGCAAAG 
TCTGTTCATT TTGAATCCAG CACCTCAGAG TCCTCAGTGC TATCAAGTAG TAGTCCAGAG 
AGTACCTTGG TGAAACCAGA GCCGAATGGC ATAACCATCC CTGGTATCTC TTCAGATGTG 
CCAGAGAGTG CCCACAAAAC TACTGCCTCA GAGGCAAAGT CAGACACTGG GCAGCCTACC 
AAGGTTGGAC GTTTTCAGGT GACAACTACA GCAAACAAAG TGGGTCGTTT CTCTGTATCA 
AAAACTGAGG ACAAGATCAC TGACACAAAG AAAGAAGGAC CAGTGGCATC TCCTCCTTTT 
ATGGATTTGG AACAAGCTGT TCTTCCTGCT GTGATACCAA AGAAAGAGAA GCCTGAACTG 
TCAGAGCCTT CACATCTAAA TGGGCCGTCT TCTGACCCGG AGGCCGCTTT TTTAAGTAGG 
GATGTGGATG ATGGTTCCGG TAGTCCACAC TCGCCCCATC AGCTGAGCTC AAAGAGCCTT 
CCTAGCCAGA ATCTAAGTCA AAGCCTTAGT AATTCATTTA ACTCCTCTTA CATGAGTAGC 
GACAATGAGT CAGATATCGA AGATGAAGAC TTAAAGTTAG AGCTGCGACG ACTACGAGAT 
AAACATCTCA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG 
TATACCAAAC TGGGCAAGGT GCCCCCTGCT GTTATTATTC CCCCAGCTGC TCCCCTTTCA 
GGGAGAAGAC GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGTCGAAG CAGTTCCTTG 
GGGAATAAAA GCCCCCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGCAGC TTCAGTCTTG 
CACCCCCAGC AGACCCTCCA CCCTCCTGGC AACATCCCAG AGTCCGGGCA GAATCAGCTG 
TTACAGCCCC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT CACCAGTGAT 
GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GAACCAGCAG CACAAACACT 
GTTGGGGCAA CAGTGAACAG CCAAGCCGCC CAAGCTCAGC CTCCTGCCAT GACGTCCAGC 
AGGAAGGGCA CATTCACAGA TGACTTGCAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC 
ATGAATCTCT CAGGCAGGAG AGGAAGCAAA GGGCACATGA ATTACGAGGG CCCTGGAATG 
GCAAGGAAGT TCTCTGCACC TGGGCAACTG TGCATCTCCA TGACCTCGAA CCTGGGTGGC 
TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC 
CCCCCACAGC AGTATGGCTT TCCAGCTACC CCATTTGGCG CTCAATGGAG TGGGACGGGT 
GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTGCAGAAT 
TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGCTCCAA CCTGCGGACC 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719066.1
CDS	969..8114
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_006719003.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719003.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG CTTCCAGTTT CCCAGCCAGT ACCAACTATC 
CAAGGCGAAC CTCAGATCCC AGTTGCGACA CAACCCTCGG TTGTTCCAGT CCACTCTGGT 
GCTCATTTCC TTCCAGTGGG ACAGCCGCTC CCTACTCCCT TGCTCCCTCA GTACCCTGTC 
TCTCAGATTC CCATATCAAC TCCTCATGTG TCTACGGCTC AGACAGGTTT CTCATCCCTT 
CCCATCACAA TGGCAGCTGG CATTACTCAG CCTCTGCTCA CGTTGGCTTC ATCTGCTACA 
ACAGCTGCGA TCCCGGGGGT ATCAACTGTG GTTCCTAGTC AGCTTCCAAC CCTTCTGCAG 
CCTGTGACTC AGCTGCCAAG TCAGGTTCAC CCACAGCTCC TACAACCAGC AGTTCAGTCC 
ATGGGAATAC CAGCTAACCT TGGACAAGCT GCTGAGGTTC CACTTTCCTC TGGAGATGTT 
CTGTACCAGG GCTTCCCACC TCGACTGCCA CCACAGTACC CAGGAGATTC AAATATTGCT 
CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTATCCCC TCCCATGCCG 
ACAGAAGTAC TGGCTACACC TGGGTACTTT CCCACAGTGG TGCAGCCTTA TGTGGAATCA 
AATCTTTTAG TTCCTATGGG TGGTGTAGGA GGACAGGTTC AAGTGTCCCA GCCAGGAGGG 
AGTTTAGCAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG TTTTGGAGAG TACTCAGGGA 
GTCTCTCAGG TTGCTCCTGC AGAGCCAGTT GCAGTAGCAC AGACCCAAGC TACCCAGCCG 
ACCACTTTGG CTTCCTCTGT AGACAGTGCA CATTCAGATG TTGCTTCAGG TATGAGTGAT 
GGCAATGAGA ACGTCCCATC TTCCAGTGGA AGGCATGAAG GAAGAACTAC AAAACGGCAT 
TACCGAAAAT CTGTAAGGAG TCGCTCTCGA CATGAAAAAA CTTCACGCCC AAAATTAAGA 
ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAATTAGA GACTCATAAT 
AGGAAAATGG TTACATTCAA ATTTGACCTA GATGGTGACA ACCCCGAGGA GATAGCAACA 
ATTATGGTGA ACAATGACTT TATTCTAGCA ATAGAGAGAG AGTCGTTTGT GGATCAAGTG 
CGAGAAATTA TTGAAAAAGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG 
GGTGATCAGG GATTGGAGAG TCTACAAGGA AAGGATGACT ATGGCTTTTC AGGTTCTCAG 
AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCGTCTT CCATGCCACA GCAAATAGGC 
ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCGG GAAGGCGGTT TATAGTGAGT 
CCTGTGCCAG AAAGCCGATT ACGAGAATCA AAAGTTTTCC CCAGTGAAAT AACAGATACA 
GTTGCTGCCT CTACAGCTCA GAGCCCTGGA ATGAACTTGT CTCACTCTGC ATCATCCCTT 
AGTCTACAAC AGGCCTTTTC TGAACTTAGA CGTGCCCAAA TGACAGAAGG ACCCAACACA 
GCACCTCCAA ACTTTAGTCA TACAGGACCA ACATTTCCAG TAGTACCTCC TTTCTTAAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGCA GCCACAGCAC CAGTCCCTGC AACAAGCAGC 
CCTCCTAATG ACATTTCCAC ATCAGTAATT CAGTCTGAGG TTACAGTGCC CACTGAAGAG 
GGGATTGCTG GAGTTGCCAC CAGCACAGGT GTGGTAACTT CAGGTGGTCT CCCCATACCA 
CCTGTGTCTG AATCACCAGT ACTTTCCAGC GTAGTTTCAA GTATCACAAT ACCTGCAGTT 
GTCTCAATAT CTACTACATC CCCGTCACTT CAAGTCCCCA CATCCACATC TGAGATCGTT 
GTTTCTAGTA CAGCACTGTA TCCTTCAGTA ACAGTTTCAG CAACTTCAGC CTCTGCAGGG 
GGCAGTACTG CTACCCCAGG TCCTAAGCCT CCAGCTGTAG TATCTCAGCA GGCAGCAGGC 
AGCACTACTG TGGGAGCCAC ATTAACATCA GTTTCTACCA CCACTTCATT CCCAAGCACA 
GCTTCACAGC TGTGCATTCA GCTTAGCAGC AGTACTTCTA CTCCTACTTT AGCTGAAACC 
GTGGTAGTTA GCGCACACTC ACTAGATAAG ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTCTCCCTCT CTGCACCATC TTCCTCTTCC TCTCCTGGAG CAGGAGTGTC TAGTTATATT 
TCTCAGCCTG GTGGGCTGCA TCCTTTGGTC ATTCCATCAG TGATAGCTTC TACTCCTATT 
CTTCCCCAAG CAGCAGGACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAGTATCCCA 
CCCTTGGTAC AGCCTGTTGC CAATGTGCCT GCTGTACAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTGCTTCC CAACCAGCCC CATACTCATT GTCCTGAAGT AGATTCTGAT 
ACACAACCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAAGAAAA GCTGCGGTCT 
CTGTTCAGTG AACACAGCTC ATCTGGAGCT CAGCATGCCT CTGTCTCACT GGAGACCTCA 
CTAGTCATAG AGAGCACTGT CACACCAGGC ATCCCAACTA CTGCTGTTGC ACCAAGCAAA 
CTCCTGACTT CTACCACAAG TACTTGCTTA CCACCAACCA ATTTACCACT AGGAACAGTT 
GCTTTGCCAG TTACACCAGT GGTCACACCT GGGCAAGTTT CTACCCCAGT CAGCACTACT 
ACATCAGGAG TGAAACCTGG AACTGCTCCC TCCAAGCCAC CTCTAACTAA GGCTCCGGTG 
CTGCCAGTGG GTACTGAACT TCCAGCAGGT ACTCTACCCA GCGAGCAGCT GCCACCTTTT 
CCAGGACCTT CTCTAACCCA GTCCCAGCAA CCTCTAGAGG ATCTTGATGC TCAATTGAGA 
AGAACACTTA GTCCAGAGAT GATCACAGTG ACTTCTGCGG TTGGTCCTGT GTCCATGGCG 
GCTCCAACAG CAATCACAGA AGCAGGAACA CAGCCTCAGA AGGGTGTTTC TCAAGTCAAA 
GAAGGCCCTG TCCTAGCAAC TAGTTCAGGA GCTGGTGTTT TTAAGATGGG ACGATTTCAG 
GTTTCTGTTG CAGCAGACGG TGCCCAGAAA GAGGGTAAAA ATAAGTCAGA AGATGCAAAG 
TCTGTTCATT TTGAATCCAG CACCTCAGAG TCCTCAGTGC TATCAAGTAG TAGTCCAGAG 
AGTACCTTGG TGAAACCAGA GCCGAATGGC ATAACCATCC CTGGTATCTC TTCAGATGTG 
CCAGAGAGTG CCCACAAAAC TACTGCCTCA GAGGCAAAGT CAGACACTGG GCAGCCTACC 
AAGGTTGGAC GTTTTCAGGT GACAACTACA GCAAACAAAG TGGGTCGTTT CTCTGTATCA 
AAAACTGAGG ACAAGATCAC TGACACAAAG AAAGAAGGAC CAGTGGCATC TCCTCCTTTT 
ATGGATTTGG AACAAGCTGT TCTTCCTGCT GTGATACCAA AGAAAGAGAA GCCTGAACTG 
TCAGAGCCTT CACATCTAAA TGGGCCGTCT TCTGACCCGG AGGCCGCTTT TTTAAGTAGG 
GATGTGGATG ATGGTTCCGG TAGTCCACAC TCGCCCCATC AGCTGAGCTC AAAGAGCCTT 
CCTAGCCAGA ATCTAAGTCA AAGCCTTAGT AATTCATTTA ACTCCTCTTA CATGAGTAGC 
GACAATGAGT CAGATATCGA AGATGAAGAC TTAAAGTTAG AGCTGCGACG ACTACGAGAT 
AAACATCTCA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG 
TATACCAAAC TGGGCAAGGT GCCCCCTGCT GTTATTATTC CCCCAGCTGC TCCCCTTTCA 
GGGAGAAGAC GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGTCGAAG CAGTTCCTTG 
GGGAATAAAA GCCCCCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGCAGC TTCAGTCTTG 
CACCCCCAGC AGACCCTCCA CCCTCCTGGC AACATCCCAG AGTCCGGGCA GAATCAGCTG 
TTACAGCCCC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT CACCAGTGAT 
GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GAACCAGCAG CACAAACACT 
GTTGGGGCAA CAGTGAACAG CCAAGCCGCC CAAGCTCAGC CTCCTGCCAT GACGTCCAGC 
AGGAAGGGCA CATTCACAGA TGACTTGCAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC 
ATGAATCTCT CAGGCAGGAG AGGAAGCAAA GGGCACATGA ATTACGAGGG CCCTGGAATG 
GCAAGGAAGT TCTCTGCACC TGGGCAACTG TGCATCTCCA TGACCTCGAA CCTGGGTGGC 
TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC 
CCCCCACAGC AGTATGGCTT TCCAGCTACC CCATTTGGCG CTCAATGGAG TGGGACGGGT 
GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTGCAGAAT 
TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGCTCCAA CCTGCGGACC 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011521002.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519304.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

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CloneID	OHu71618
Clone ID Related Accession (Same CDS sequence)	XM_011521002.2
Accession Version	XM_011521002.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521002.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGG GCTTCCCACC TCGACTGCCA CCACAGTACC CAGGAGATTC AAATATTGCT 
CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTATCCCC TCCCATGCCG 
ACAGAAGTAC TGGCTACACC TGGGTACTTT CCCACAGTGG TGCAGCCTTA TGTGGAATCA 
AATCTTTTAG TTCCTATGGG TGGTGTAGGA GGACAGGTTC AAGTGTCCCA GCCAGGAGGG 
AGTTTAGCAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG TTTTGGAGAG TACTCAGGGA 
GTCTCTCAGG TTGCTCCTGC AGAGCCAGTT GCAGTAGCAC AGACCCAAGC TACCCAGCCG 
ACCACTTTGG CTTCCTCTGT AGACAGTGCA CATTCAGATG TTGCTTCAGG TATGAGTGAT 
GGCAATGAGA ACGTCCCATC TTCCAGTGGA AGGCATGAAG GAAGAACTAC AAAACGGCAT 
TACCGAAAAT CTGTAAGGAG TCGCTCTCGA CATGAAAAAA CTTCACGCCC AAAATTAAGA 
ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAATTAGA GACTCATAAT 
AGGAAAATGG TTACATTCAA ATTTGACCTA GATGGTGACA ACCCCGAGGA GATAGCAACA 
ATTATGGTGA ACAATGACTT TATTCTAGCA ATAGAGAGAG AGTCGTTTGT GGATCAAGTG 
CGAGAAATTA TTGAAAAAGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG 
GGTGATCAGG GATTGGAGAG TCTACAAGGA AAGGATGACT ATGGCTTTTC AGGTTCTCAG 
AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCGTCTT CCATGCCACA GCAAATAGGC 
ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCGG GAAGGCGGTT TATAGTGAGT 
CCTGTGCCAG AAAGCCGATT ACGAGAATCA AAAGTTTTCC CCAGTGAAAT AACAGATACA 
GTTGCTGCCT CTACAGCTCA GAGCCCTGGA ATGAACTTGT CTCACTCTGC ATCATCCCTT 
AGTCTACAAC AGGCCTTTTC TGAACTTAGA CGTGCCCAAA TGACAGAAGG ACCCAACACA 
GCACCTCCAA ACTTTAGTCA TACAGGACCA ACATTTCCAG TAGTACCTCC TTTCTTAAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGCA GCCACAGCAC CAGTCCCTGC AACAAGCAGC 
CCTCCTAATG ACATTTCCAC ATCAGTAATT CAGTCTGAGG TTACAGTGCC CACTGAAGAG 
GGGATTGCTG GAGTTGCCAC CAGCACAGGT GTGGTAACTT CAGGTGGTCT CCCCATACCA 
CCTGTGTCTG AATCACCAGT ACTTTCCAGC GTAGTTTCAA GTATCACAAT ACCTGCAGTT 
GTCTCAATAT CTACTACATC CCCGTCACTT CAAGTCCCCA CATCCACATC TGAGATCGTT 
GTTTCTAGTA CAGCACTGTA TCCTTCAGTA ACAGTTTCAG CAACTTCAGC CTCTGCAGGG 
GGCAGTACTG CTACCCCAGG TCCTAAGCCT CCAGCTGTAG TATCTCAGCA GGCAGCAGGC 
AGCACTACTG TGGGAGCCAC ATTAACATCA GTTTCTACCA CCACTTCATT CCCAAGCACA 
GCTTCACAGC TGTGCATTCA GCTTAGCAGC AGTACTTCTA CTCCTACTTT AGCTGAAACC 
GTGGTAGTTA GCGCACACTC ACTAGATAAG ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTCTCCCTCT CTGCACCATC TTCCTCTTCC TCTCCTGGAG CAGGAGTGTC TAGTTATATT 
TCTCAGCCTG GTGGGCTGCA TCCTTTGGTC ATTCCATCAG TGATAGCTTC TACTCCTATT 
CTTCCCCAAG CAGCAGGACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAGTATCCCA 
CCCTTGGTAC AGCCTGTTGC CAATGTGCCT GCTGTACAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTGCTTCC CAACCAGCCC CATACTCATT GTCCTGAAGT AGATTCTGAT 
ACACAACCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAAGAAAA GCTGCGGTCT 
CTGTTCAGTG AACACAGCTC ATCTGGAGCT CAGCATGCCT CTGTCTCACT GGAGACCTCA 
CTAGTCATAG AGAGCACTGT CACACCAGGC ATCCCAACTA CTGCTGTTGC ACCAAGCAAA 
CTCCTGACTT CTACCACAAG TACTTGCTTA CCACCAACCA ATTTACCACT AGGAACAGTT 
GCTTTGCCAG TTACACCAGT GGTCACACCT GGGCAAGTTT CTACCCCAGT CAGCACTACT 
ACATCAGGAG TGAAACCTGG AACTGCTCCC TCCAAGCCAC CTCTAACTAA GGCTCCGGTG 
CTGCCAGTGG GTACTGAACT TCCAGCAGGT ACTCTACCCA GCGAGCAGCT GCCACCTTTT 
CCAGGACCTT CTCTAACCCA GTCCCAGCAA CCTCTAGAGG ATCTTGATGC TCAATTGAGA 
AGAACACTTA GTCCAGAGAT GATCACAGTG ACTTCTGCGG TTGGTCCTGT GTCCATGGCG 
GCTCCAACAG CAATCACAGA AGCAGGAACA CAGCCTCAGA AGGGTGTTTC TCAAGTCAAA 
GAAGGCCCTG TCCTAGCAAC TAGTTCAGGA GCTGGTGTTT TTAAGATGGG ACGATTTCAG 
GTTTCTGTTG CAGCAGACGG TGCCCAGAAA GAGGGTAAAA ATAAGTCAGA AGATGCAAAG 
TCTGTTCATT TTGAATCCAG CACCTCAGAG TCCTCAGTGC TATCAAGTAG TAGTCCAGAG 
AGTACCTTGG TGAAACCAGA GCCGAATGGC ATAACCATCC CTGGTATCTC TTCAGATGTG 
CCAGAGAGTG CCCACAAAAC TACTGCCTCA GAGGCAAAGT CAGACACTGG GCAGCCTACC 
AAGGTTGGAC GTTTTCAGGT GACAACTACA GCAAACAAAG TGGGTCGTTT CTCTGTATCA 
AAAACTGAGG ACAAGATCAC TGACACAAAG AAAGAAGGAC CAGTGGCATC TCCTCCTTTT 
ATGGATTTGG AACAAGCTGT TCTTCCTGCT GTGATACCAA AGAAAGAGAA GCCTGAACTG 
TCAGAGCCTT CACATCTAAA TGGGCCGTCT TCTGACCCGG AGGCCGCTTT TTTAAGTAGG 
GATGTGGATG ATGGTTCCGG TAGTCCACAC TCGCCCCATC AGCTGAGCTC AAAGAGCCTT 
CCTAGCCAGA ATCTAAGTCA AAGCCTTAGT AATTCATTTA ACTCCTCTTA CATGAGTAGC 
GACAATGAGT CAGATATCGA AGATGAAGAC TTAAAGTTAG AGCTGCGACG ACTACGAGAT 
AAACATCTCA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG 
TATACCAAAC TGGGCAAGGT GCCCCCTGCT GTTATTATTC CCCCAGCTGC TCCCCTTTCA 
GGGAGAAGAC GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGTCGAAG CAGTTCCTTG 
GGGAATAAAA GCCCCCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGCAGC TTCAGTCTTG 
CACCCCCAGC AGACCCTCCA CCCTCCTGGC AACATCCCAG AGTCCGGGCA GAATCAGCTG 
TTACAGCCCC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT CACCAGTGAT 
GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GAACCAGCAG CACAAACACT 
GTTGGGGCAA CAGTGAACAG CCAAGCCGCC CAAGCTCAGC CTCCTGCCAT GACGTCCAGC 
AGGAAGGGCA CATTCACAGA TGACTTGCAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC 
ATGAATCTCT CAGGCAGGAG AGGAAGCAAA GGGCACATGA ATTACGAGGG CCCTGGAATG 
GCAAGGAAGT TCTCTGCACC TGGGCAACTG TGCATCTCCA TGACCTCGAA CCTGGGTGGC 
TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC 
CCCCCACAGC AGTATGGCTT TCCAGCTACC CCATTTGGCG CTCAATGGAG TGGGACGGGT 
GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTGCAGAAT 
TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGCTCCAA CCTGCGGACC 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519304.1
CDS	969..8894
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQAQGQSQGQPSSSSLTGVSSSQPIQHPQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011521002.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521002.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGG GCTTCCCACC TCGACTGCCA CCACAGTACC CAGGAGATTC AAATATTGCT 
CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTATCCCC TCCCATGCCG 
ACAGAAGTAC TGGCTACACC TGGGTACTTT CCCACAGTGG TGCAGCCTTA TGTGGAATCA 
AATCTTTTAG TTCCTATGGG TGGTGTAGGA GGACAGGTTC AAGTGTCCCA GCCAGGAGGG 
AGTTTAGCAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG TTTTGGAGAG TACTCAGGGA 
GTCTCTCAGG TTGCTCCTGC AGAGCCAGTT GCAGTAGCAC AGACCCAAGC TACCCAGCCG 
ACCACTTTGG CTTCCTCTGT AGACAGTGCA CATTCAGATG TTGCTTCAGG TATGAGTGAT 
GGCAATGAGA ACGTCCCATC TTCCAGTGGA AGGCATGAAG GAAGAACTAC AAAACGGCAT 
TACCGAAAAT CTGTAAGGAG TCGCTCTCGA CATGAAAAAA CTTCACGCCC AAAATTAAGA 
ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAATTAGA GACTCATAAT 
AGGAAAATGG TTACATTCAA ATTTGACCTA GATGGTGACA ACCCCGAGGA GATAGCAACA 
ATTATGGTGA ACAATGACTT TATTCTAGCA ATAGAGAGAG AGTCGTTTGT GGATCAAGTG 
CGAGAAATTA TTGAAAAAGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG 
GGTGATCAGG GATTGGAGAG TCTACAAGGA AAGGATGACT ATGGCTTTTC AGGTTCTCAG 
AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCGTCTT CCATGCCACA GCAAATAGGC 
ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCGG GAAGGCGGTT TATAGTGAGT 
CCTGTGCCAG AAAGCCGATT ACGAGAATCA AAAGTTTTCC CCAGTGAAAT AACAGATACA 
GTTGCTGCCT CTACAGCTCA GAGCCCTGGA ATGAACTTGT CTCACTCTGC ATCATCCCTT 
AGTCTACAAC AGGCCTTTTC TGAACTTAGA CGTGCCCAAA TGACAGAAGG ACCCAACACA 
GCACCTCCAA ACTTTAGTCA TACAGGACCA ACATTTCCAG TAGTACCTCC TTTCTTAAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGCA GCCACAGCAC CAGTCCCTGC AACAAGCAGC 
CCTCCTAATG ACATTTCCAC ATCAGTAATT CAGTCTGAGG TTACAGTGCC CACTGAAGAG 
GGGATTGCTG GAGTTGCCAC CAGCACAGGT GTGGTAACTT CAGGTGGTCT CCCCATACCA 
CCTGTGTCTG AATCACCAGT ACTTTCCAGC GTAGTTTCAA GTATCACAAT ACCTGCAGTT 
GTCTCAATAT CTACTACATC CCCGTCACTT CAAGTCCCCA CATCCACATC TGAGATCGTT 
GTTTCTAGTA CAGCACTGTA TCCTTCAGTA ACAGTTTCAG CAACTTCAGC CTCTGCAGGG 
GGCAGTACTG CTACCCCAGG TCCTAAGCCT CCAGCTGTAG TATCTCAGCA GGCAGCAGGC 
AGCACTACTG TGGGAGCCAC ATTAACATCA GTTTCTACCA CCACTTCATT CCCAAGCACA 
GCTTCACAGC TGTGCATTCA GCTTAGCAGC AGTACTTCTA CTCCTACTTT AGCTGAAACC 
GTGGTAGTTA GCGCACACTC ACTAGATAAG ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTCTCCCTCT CTGCACCATC TTCCTCTTCC TCTCCTGGAG CAGGAGTGTC TAGTTATATT 
TCTCAGCCTG GTGGGCTGCA TCCTTTGGTC ATTCCATCAG TGATAGCTTC TACTCCTATT 
CTTCCCCAAG CAGCAGGACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAGTATCCCA 
CCCTTGGTAC AGCCTGTTGC CAATGTGCCT GCTGTACAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTGCTTCC CAACCAGCCC CATACTCATT GTCCTGAAGT AGATTCTGAT 
ACACAACCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAAGAAAA GCTGCGGTCT 
CTGTTCAGTG AACACAGCTC ATCTGGAGCT CAGCATGCCT CTGTCTCACT GGAGACCTCA 
CTAGTCATAG AGAGCACTGT CACACCAGGC ATCCCAACTA CTGCTGTTGC ACCAAGCAAA 
CTCCTGACTT CTACCACAAG TACTTGCTTA CCACCAACCA ATTTACCACT AGGAACAGTT 
GCTTTGCCAG TTACACCAGT GGTCACACCT GGGCAAGTTT CTACCCCAGT CAGCACTACT 
ACATCAGGAG TGAAACCTGG AACTGCTCCC TCCAAGCCAC CTCTAACTAA GGCTCCGGTG 
CTGCCAGTGG GTACTGAACT TCCAGCAGGT ACTCTACCCA GCGAGCAGCT GCCACCTTTT 
CCAGGACCTT CTCTAACCCA GTCCCAGCAA CCTCTAGAGG ATCTTGATGC TCAATTGAGA 
AGAACACTTA GTCCAGAGAT GATCACAGTG ACTTCTGCGG TTGGTCCTGT GTCCATGGCG 
GCTCCAACAG CAATCACAGA AGCAGGAACA CAGCCTCAGA AGGGTGTTTC TCAAGTCAAA 
GAAGGCCCTG TCCTAGCAAC TAGTTCAGGA GCTGGTGTTT TTAAGATGGG ACGATTTCAG 
GTTTCTGTTG CAGCAGACGG TGCCCAGAAA GAGGGTAAAA ATAAGTCAGA AGATGCAAAG 
TCTGTTCATT TTGAATCCAG CACCTCAGAG TCCTCAGTGC TATCAAGTAG TAGTCCAGAG 
AGTACCTTGG TGAAACCAGA GCCGAATGGC ATAACCATCC CTGGTATCTC TTCAGATGTG 
CCAGAGAGTG CCCACAAAAC TACTGCCTCA GAGGCAAAGT CAGACACTGG GCAGCCTACC 
AAGGTTGGAC GTTTTCAGGT GACAACTACA GCAAACAAAG TGGGTCGTTT CTCTGTATCA 
AAAACTGAGG ACAAGATCAC TGACACAAAG AAAGAAGGAC CAGTGGCATC TCCTCCTTTT 
ATGGATTTGG AACAAGCTGT TCTTCCTGCT GTGATACCAA AGAAAGAGAA GCCTGAACTG 
TCAGAGCCTT CACATCTAAA TGGGCCGTCT TCTGACCCGG AGGCCGCTTT TTTAAGTAGG 
GATGTGGATG ATGGTTCCGG TAGTCCACAC TCGCCCCATC AGCTGAGCTC AAAGAGCCTT 
CCTAGCCAGA ATCTAAGTCA AAGCCTTAGT AATTCATTTA ACTCCTCTTA CATGAGTAGC 
GACAATGAGT CAGATATCGA AGATGAAGAC TTAAAGTTAG AGCTGCGACG ACTACGAGAT 
AAACATCTCA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG 
TATACCAAAC TGGGCAAGGT GCCCCCTGCT GTTATTATTC CCCCAGCTGC TCCCCTTTCA 
GGGAGAAGAC GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGTCGAAG CAGTTCCTTG 
GGGAATAAAA GCCCCCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGCAGC TTCAGTCTTG 
CACCCCCAGC AGACCCTCCA CCCTCCTGGC AACATCCCAG AGTCCGGGCA GAATCAGCTG 
TTACAGCCCC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT CACCAGTGAT 
GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GAACCAGCAG CACAAACACT 
GTTGGGGCAA CAGTGAACAG CCAAGCCGCC CAAGCTCAGC CTCCTGCCAT GACGTCCAGC 
AGGAAGGGCA CATTCACAGA TGACTTGCAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC 
ATGAATCTCT CAGGCAGGAG AGGAAGCAAA GGGCACATGA ATTACGAGGG CCCTGGAATG 
GCAAGGAAGT TCTCTGCACC TGGGCAACTG TGCATCTCCA TGACCTCGAA CCTGGGTGGC 
TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC 
CCCCCACAGC AGTATGGCTT TCCAGCTACC CCATTTGGCG CTCAATGGAG TGGGACGGGT 
GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTGCAGAAT 
TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGCTCCAA CCTGCGGACC 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011521003.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519305.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

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CloneID	OHu71619
Clone ID Related Accession (Same CDS sequence)	XM_011521003.2
Accession Version	XM_011521003.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGAGTACTCA GGGAGTCTCT CAGGTTGCTC CTGCAGAGCC AGTTGCAGTA 
GCACAGACCC AAGCTACCCA GCCGACCACT TTGGCTTCCT CTGTAGACAG TGCACATTCA 
GATGTTGCTT CAGGTATGAG TGATGGCAAT GAGAACGTCC CATCTTCCAG TGGAAGGCAT 
GAAGGAAGAA CTACAAAACG GCATTACCGA AAATCTGTAA GGAGTCGCTC TCGACATGAA 
AAAACTTCAC GCCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA 
GAATGTCAAT TAGAGACTCA TAATAGGAAA ATGGTTACAT TCAAATTTGA CCTAGATGGT 
GACAACCCCG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATAGAG 
AGAGAGTCGT TTGTGGATCA AGTGCGAGAA ATTATTGAAA AAGCTGATGA AATGCTCAGT 
GAGGATGTCA GTGTGGAACC AGAGGGTGAT CAGGGATTGG AGAGTCTACA AGGAAAGGAT 
GACTATGGCT TTTCAGGTTC TCAGAAATTG GAAGGAGAGT TCAAACAACC AATTCCTGCG 
TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT TGTTCATTCT 
GCGGGAAGGC GGTTTATAGT GAGTCCTGTG CCAGAAAGCC GATTACGAGA ATCAAAAGTT 
TTCCCCAGTG AAATAACAGA TACAGTTGCT GCCTCTACAG CTCAGAGCCC TGGAATGAAC 
TTGTCTCACT CTGCATCATC CCTTAGTCTA CAACAGGCCT TTTCTGAACT TAGACGTGCC 
CAAATGACAG AAGGACCCAA CACAGCACCT CCAAACTTTA GTCATACAGG ACCAACATTT 
CCAGTAGTAC CTCCTTTCTT AAGTAGCATT GCTGGAGTCC CAACCACAGC AGCAGCCACA 
GCACCAGTCC CTGCAACAAG CAGCCCTCCT AATGACATTT CCACATCAGT AATTCAGTCT 
GAGGTTACAG TGCCCACTGA AGAGGGGATT GCTGGAGTTG CCACCAGCAC AGGTGTGGTA 
ACTTCAGGTG GTCTCCCCAT ACCACCTGTG TCTGAATCAC CAGTACTTTC CAGCGTAGTT 
TCAAGTATCA CAATACCTGC AGTTGTCTCA ATATCTACTA CATCCCCGTC ACTTCAAGTC 
CCCACATCCA CATCTGAGAT CGTTGTTTCT AGTACAGCAC TGTATCCTTC AGTAACAGTT 
TCAGCAACTT CAGCCTCTGC AGGGGGCAGT ACTGCTACCC CAGGTCCTAA GCCTCCAGCT 
GTAGTATCTC AGCAGGCAGC AGGCAGCACT ACTGTGGGAG CCACATTAAC ATCAGTTTCT 
ACCACCACTT CATTCCCAAG CACAGCTTCA CAGCTGTGCA TTCAGCTTAG CAGCAGTACT 
TCTACTCCTA CTTTAGCTGA AACCGTGGTA GTTAGCGCAC ACTCACTAGA TAAGACATCT 
CATAGCAGTA CAACTGGATT GGCTTTCTCC CTCTCTGCAC CATCTTCCTC TTCCTCTCCT 
GGAGCAGGAG TGTCTAGTTA TATTTCTCAG CCTGGTGGGC TGCATCCTTT GGTCATTCCA 
TCAGTGATAG CTTCTACTCC TATTCTTCCC CAAGCAGCAG GACCTACTTC TACACCTTTA 
TTACCCCAAG TACCTAGTAT CCCACCCTTG GTACAGCCTG TTGCCAATGT GCCTGCTGTA 
CAGCAGACAC TAATTCATAG TCAGCCTCAA CCAGCTTTGC TTCCCAACCA GCCCCATACT 
CATTGTCCTG AAGTAGATTC TGATACACAA CCCAAAGCTC CTGGAATTGA TGACATAAAG 
ACTCTAGAAG AAAAGCTGCG GTCTCTGTTC AGTGAACACA GCTCATCTGG AGCTCAGCAT 
GCCTCTGTCT CACTGGAGAC CTCACTAGTC ATAGAGAGCA CTGTCACACC AGGCATCCCA 
ACTACTGCTG TTGCACCAAG CAAACTCCTG ACTTCTACCA CAAGTACTTG CTTACCACCA 
ACCAATTTAC CACTAGGAAC AGTTGCTTTG CCAGTTACAC CAGTGGTCAC ACCTGGGCAA 
GTTTCTACCC CAGTCAGCAC TACTACATCA GGAGTGAAAC CTGGAACTGC TCCCTCCAAG 
CCACCTCTAA CTAAGGCTCC GGTGCTGCCA GTGGGTACTG AACTTCCAGC AGGTACTCTA 
CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCTCTAA CCCAGTCCCA GCAACCTCTA 
GAGGATCTTG ATGCTCAATT GAGAAGAACA CTTAGTCCAG AGATGATCAC AGTGACTTCT 
GCGGTTGGTC CTGTGTCCAT GGCGGCTCCA ACAGCAATCA CAGAAGCAGG AACACAGCCT 
CAGAAGGGTG TTTCTCAAGT CAAAGAAGGC CCTGTCCTAG CAACTAGTTC AGGAGCTGGT 
GTTTTTAAGA TGGGACGATT TCAGGTTTCT GTTGCAGCAG ACGGTGCCCA GAAAGAGGGT 
AAAAATAAGT CAGAAGATGC AAAGTCTGTT CATTTTGAAT CCAGCACCTC AGAGTCCTCA 
GTGCTATCAA GTAGTAGTCC AGAGAGTACC TTGGTGAAAC CAGAGCCGAA TGGCATAACC 
ATCCCTGGTA TCTCTTCAGA TGTGCCAGAG AGTGCCCACA AAACTACTGC CTCAGAGGCA 
AAGTCAGACA CTGGGCAGCC TACCAAGGTT GGACGTTTTC AGGTGACAAC TACAGCAAAC 
AAAGTGGGTC GTTTCTCTGT ATCAAAAACT GAGGACAAGA TCACTGACAC AAAGAAAGAA 
GGACCAGTGG CATCTCCTCC TTTTATGGAT TTGGAACAAG CTGTTCTTCC TGCTGTGATA 
CCAAAGAAAG AGAAGCCTGA ACTGTCAGAG CCTTCACATC TAAATGGGCC GTCTTCTGAC 
CCGGAGGCCG CTTTTTTAAG TAGGGATGTG GATGATGGTT CCGGTAGTCC ACACTCGCCC 
CATCAGCTGA GCTCAAAGAG CCTTCCTAGC CAGAATCTAA GTCAAAGCCT TAGTAATTCA 
TTTAACTCCT CTTACATGAG TAGCGACAAT GAGTCAGATA TCGAAGATGA AGACTTAAAG 
TTAGAGCTGC GACGACTACG AGATAAACAT CTCAAAGAGA TTCAGGACCT GCAGAGTCGC 
CAGAAGCATG AAATTGAATC TTTGTATACC AAACTGGGCA AGGTGCCCCC TGCTGTTATT 
ATTCCCCCAG CTGCTCCCCT TTCAGGGAGA AGACGACGAC CCACTAAAAG CAAAGGCAGC 
AAATCTAGTC GAAGCAGTTC CTTGGGGAAT AAAAGCCCCC AGCTTTCAGG TAACCTGTCT 
GGTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACCC TCCACCCTCC TGGCAACATC 
CCAGAGTCCG GGCAGAATCA GCTGTTACAG CCCCTTAAGC CATCTCCCTC CAGTGACAAC 
CTCTATTCAG CCTTCACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGT 
CAAGGAACCA GCAGCACAAA CACTGTTGGG GCAACAGTGA ACAGCCAAGC CGCCCAAGCT 
CAGCCTCCTG CCATGACGTC CAGCAGGAAG GGCACATTCA CAGATGACTT GCACAAGTTG 
GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAAAGGGCAC 
ATGAATTACG AGGGCCCTGG AATGGCAAGG AAGTTCTCTG CACCTGGGCA ACTGTGCATC 
TCCATGACCT CGAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA 
GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTATG GCTTTCCAGC TACCCCATTT 
GGCGCTCAAT GGAGTGGGAC GGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT 
GTGGGAACTG CCTCCTTGCA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC 
CCCCCAGGCT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519305.1
CDS	969..8618
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011521003.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521003.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGAGTACTCA GGGAGTCTCT CAGGTTGCTC CTGCAGAGCC AGTTGCAGTA 
GCACAGACCC AAGCTACCCA GCCGACCACT TTGGCTTCCT CTGTAGACAG TGCACATTCA 
GATGTTGCTT CAGGTATGAG TGATGGCAAT GAGAACGTCC CATCTTCCAG TGGAAGGCAT 
GAAGGAAGAA CTACAAAACG GCATTACCGA AAATCTGTAA GGAGTCGCTC TCGACATGAA 
AAAACTTCAC GCCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA 
GAATGTCAAT TAGAGACTCA TAATAGGAAA ATGGTTACAT TCAAATTTGA CCTAGATGGT 
GACAACCCCG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATAGAG 
AGAGAGTCGT TTGTGGATCA AGTGCGAGAA ATTATTGAAA AAGCTGATGA AATGCTCAGT 
GAGGATGTCA GTGTGGAACC AGAGGGTGAT CAGGGATTGG AGAGTCTACA AGGAAAGGAT 
GACTATGGCT TTTCAGGTTC TCAGAAATTG GAAGGAGAGT TCAAACAACC AATTCCTGCG 
TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT TGTTCATTCT 
GCGGGAAGGC GGTTTATAGT GAGTCCTGTG CCAGAAAGCC GATTACGAGA ATCAAAAGTT 
TTCCCCAGTG AAATAACAGA TACAGTTGCT GCCTCTACAG CTCAGAGCCC TGGAATGAAC 
TTGTCTCACT CTGCATCATC CCTTAGTCTA CAACAGGCCT TTTCTGAACT TAGACGTGCC 
CAAATGACAG AAGGACCCAA CACAGCACCT CCAAACTTTA GTCATACAGG ACCAACATTT 
CCAGTAGTAC CTCCTTTCTT AAGTAGCATT GCTGGAGTCC CAACCACAGC AGCAGCCACA 
GCACCAGTCC CTGCAACAAG CAGCCCTCCT AATGACATTT CCACATCAGT AATTCAGTCT 
GAGGTTACAG TGCCCACTGA AGAGGGGATT GCTGGAGTTG CCACCAGCAC AGGTGTGGTA 
ACTTCAGGTG GTCTCCCCAT ACCACCTGTG TCTGAATCAC CAGTACTTTC CAGCGTAGTT 
TCAAGTATCA CAATACCTGC AGTTGTCTCA ATATCTACTA CATCCCCGTC ACTTCAAGTC 
CCCACATCCA CATCTGAGAT CGTTGTTTCT AGTACAGCAC TGTATCCTTC AGTAACAGTT 
TCAGCAACTT CAGCCTCTGC AGGGGGCAGT ACTGCTACCC CAGGTCCTAA GCCTCCAGCT 
GTAGTATCTC AGCAGGCAGC AGGCAGCACT ACTGTGGGAG CCACATTAAC ATCAGTTTCT 
ACCACCACTT CATTCCCAAG CACAGCTTCA CAGCTGTGCA TTCAGCTTAG CAGCAGTACT 
TCTACTCCTA CTTTAGCTGA AACCGTGGTA GTTAGCGCAC ACTCACTAGA TAAGACATCT 
CATAGCAGTA CAACTGGATT GGCTTTCTCC CTCTCTGCAC CATCTTCCTC TTCCTCTCCT 
GGAGCAGGAG TGTCTAGTTA TATTTCTCAG CCTGGTGGGC TGCATCCTTT GGTCATTCCA 
TCAGTGATAG CTTCTACTCC TATTCTTCCC CAAGCAGCAG GACCTACTTC TACACCTTTA 
TTACCCCAAG TACCTAGTAT CCCACCCTTG GTACAGCCTG TTGCCAATGT GCCTGCTGTA 
CAGCAGACAC TAATTCATAG TCAGCCTCAA CCAGCTTTGC TTCCCAACCA GCCCCATACT 
CATTGTCCTG AAGTAGATTC TGATACACAA CCCAAAGCTC CTGGAATTGA TGACATAAAG 
ACTCTAGAAG AAAAGCTGCG GTCTCTGTTC AGTGAACACA GCTCATCTGG AGCTCAGCAT 
GCCTCTGTCT CACTGGAGAC CTCACTAGTC ATAGAGAGCA CTGTCACACC AGGCATCCCA 
ACTACTGCTG TTGCACCAAG CAAACTCCTG ACTTCTACCA CAAGTACTTG CTTACCACCA 
ACCAATTTAC CACTAGGAAC AGTTGCTTTG CCAGTTACAC CAGTGGTCAC ACCTGGGCAA 
GTTTCTACCC CAGTCAGCAC TACTACATCA GGAGTGAAAC CTGGAACTGC TCCCTCCAAG 
CCACCTCTAA CTAAGGCTCC GGTGCTGCCA GTGGGTACTG AACTTCCAGC AGGTACTCTA 
CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCTCTAA CCCAGTCCCA GCAACCTCTA 
GAGGATCTTG ATGCTCAATT GAGAAGAACA CTTAGTCCAG AGATGATCAC AGTGACTTCT 
GCGGTTGGTC CTGTGTCCAT GGCGGCTCCA ACAGCAATCA CAGAAGCAGG AACACAGCCT 
CAGAAGGGTG TTTCTCAAGT CAAAGAAGGC CCTGTCCTAG CAACTAGTTC AGGAGCTGGT 
GTTTTTAAGA TGGGACGATT TCAGGTTTCT GTTGCAGCAG ACGGTGCCCA GAAAGAGGGT 
AAAAATAAGT CAGAAGATGC AAAGTCTGTT CATTTTGAAT CCAGCACCTC AGAGTCCTCA 
GTGCTATCAA GTAGTAGTCC AGAGAGTACC TTGGTGAAAC CAGAGCCGAA TGGCATAACC 
ATCCCTGGTA TCTCTTCAGA TGTGCCAGAG AGTGCCCACA AAACTACTGC CTCAGAGGCA 
AAGTCAGACA CTGGGCAGCC TACCAAGGTT GGACGTTTTC AGGTGACAAC TACAGCAAAC 
AAAGTGGGTC GTTTCTCTGT ATCAAAAACT GAGGACAAGA TCACTGACAC AAAGAAAGAA 
GGACCAGTGG CATCTCCTCC TTTTATGGAT TTGGAACAAG CTGTTCTTCC TGCTGTGATA 
CCAAAGAAAG AGAAGCCTGA ACTGTCAGAG CCTTCACATC TAAATGGGCC GTCTTCTGAC 
CCGGAGGCCG CTTTTTTAAG TAGGGATGTG GATGATGGTT CCGGTAGTCC ACACTCGCCC 
CATCAGCTGA GCTCAAAGAG CCTTCCTAGC CAGAATCTAA GTCAAAGCCT TAGTAATTCA 
TTTAACTCCT CTTACATGAG TAGCGACAAT GAGTCAGATA TCGAAGATGA AGACTTAAAG 
TTAGAGCTGC GACGACTACG AGATAAACAT CTCAAAGAGA TTCAGGACCT GCAGAGTCGC 
CAGAAGCATG AAATTGAATC TTTGTATACC AAACTGGGCA AGGTGCCCCC TGCTGTTATT 
ATTCCCCCAG CTGCTCCCCT TTCAGGGAGA AGACGACGAC CCACTAAAAG CAAAGGCAGC 
AAATCTAGTC GAAGCAGTTC CTTGGGGAAT AAAAGCCCCC AGCTTTCAGG TAACCTGTCT 
GGTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACCC TCCACCCTCC TGGCAACATC 
CCAGAGTCCG GGCAGAATCA GCTGTTACAG CCCCTTAAGC CATCTCCCTC CAGTGACAAC 
CTCTATTCAG CCTTCACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGT 
CAAGGAACCA GCAGCACAAA CACTGTTGGG GCAACAGTGA ACAGCCAAGC CGCCCAAGCT 
CAGCCTCCTG CCATGACGTC CAGCAGGAAG GGCACATTCA CAGATGACTT GCACAAGTTG 
GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAAAGGGCAC 
ATGAATTACG AGGGCCCTGG AATGGCAAGG AAGTTCTCTG CACCTGGGCA ACTGTGCATC 
TCCATGACCT CGAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA 
GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTATG GCTTTCCAGC TACCCCATTT 
GGCGCTCAAT GGAGTGGGAC GGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT 
GTGGGAACTG CCTCCTTGCA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC 
CCCCCAGGCT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011521004.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519306.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

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CloneID	OHu71620
Clone ID Related Accession (Same CDS sequence)	XM_011521004.2
Accession Version	XM_011521004.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA 
CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA 
GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA 
ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA 
TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC 
AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA 
GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC 
TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT 
TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG 
GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC 
CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG 
TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA 
ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA 
GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA 
CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG 
GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT 
TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA 
AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC 
ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA 
GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA 
GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC 
ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT 
ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA 
GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA 
GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA 
CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT 
TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC 
TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT 
ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC 
AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT 
TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA 
CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC 
AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG 
GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG 
GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG 
AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT 
TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA 
AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG 
CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC 
CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG 
TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA 
GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA 
CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA 
AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG 
GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT 
CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT 
AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA 
GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG 
AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT 
CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA 
TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT 
CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA 
GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC 
TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA 
GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG 
AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC 
ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC 
GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGCTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519306.1
CDS	969..8615
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQAQGQSQGQPSSSSLTGVSSSQPIQHPQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011521004.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521004.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA 
CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA 
GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA 
ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA 
TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC 
AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA 
GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC 
TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT 
TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG 
GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC 
CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG 
TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA 
ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA 
GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA 
CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG 
GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT 
TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA 
AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC 
ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA 
GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA 
GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC 
ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT 
ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA 
GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA 
GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA 
CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT 
TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC 
TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT 
ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC 
AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT 
TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA 
CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC 
AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG 
GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG 
GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG 
AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT 
TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA 
AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG 
CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC 
CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG 
TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA 
GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA 
CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA 
AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG 
GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT 
CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT 
AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA 
GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG 
AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT 
CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA 
TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT 
CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA 
GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC 
TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA 
GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG 
AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC 
ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC 
GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGCTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011521005.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519307.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

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CloneID	OHu71621
Clone ID Related Accession (Same CDS sequence)	XM_011521005.2
Accession Version	XM_011521005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA AAAAGAAAGA TTTTTGCTCA GTATTTGTCA TCATAAATTC TCATTGCTGC 
TGCTGCCCTC AAAAGGATTG TATAAATGAG GGGGTGAAGC CAGCCAGTTT TGACAAAGTA 
GCAATTCCTG AAGTGAAGGA AATTATTGAA GGATGCATAC GACAAAACAA AGATGAAAGA 
TATTCCATCA AAGACCTTTT GAACCATGCC TTCTTCCAAG AGGAAACAGG AGTACGGGTA 
GAATTAGCAG AAGAAGATGA TGGAGAAAAA ATAGCCATAA AATTATGGCT ACGTATTGAA 
GATATTAAGA AATTAAAGGG AAAATACAAA GATAATGAAG CTATTGAGTT TTCTTTTGAT 
TTAGAGAGAG ATGTCCCAGA AGATGTTGCA CAAGAAATGG TAGAGTCTGG GTATGTCTGT 
GAAGGTGATC ACAAGACCAT GGCTAAAGCT ATCAAAGACA GAGTATCATT AATTAAGAGG 
AAACGAGAGC AGCGGCAGTT GGTACGGGAG GAGCAAGAAA AAAAAAAGCA GGAAGAGAGC 
AGTCTCAAAC AGCAGGTAGA ACAATCCAGT GCTTCCCAGA CAGGAATCAA GCAGCTCCCT 
TCTGCTAGCA CCGGCATACC TACTGCTTCT ACCACTTCAG CTTCAGTTTC TACACAAGTA 
GAACCTGAAG AACCTGAGGC AGATCAACAT CAACAACTAC AGTACCAGCA ACCCAGTATA 
TCTGTGTTAT CTGATGGGAC GGTTGACAGT GGTCAGGGAT CCTCTGTCTT CACAGAATCT 
CGAGTGAGCA GCCAACAGAC AGTTTCATAT GGTTCCCAAC ATGAACAGGC ACATTCTACA 
GGCACAGTCC CAGGGCATAT ACCTTCTACT GTCCAAGCAC AGTCTCAGCC CCATGGGGTA 
TATCCACCCT CAAGTGTGCC TCAGTCCATG GCGCATCCGT GTGGGGGGAC CCCAACATAC 
CCAGAATCAC AGATATTTTT CCCAACTATT CATGAACGTC CAGTTTCTTT TTCACCACCT 
CCCACCTGCC CACCGAAAGT AGCCATTTCC CAGCGGCGTA AGAGCACCTC CTTCCTGGAA 
GCCCAAACTC ACCACTTCCA ACCCCTGCTG AGGACTGTTG GCCAAAGTCT TCTTCCACCT 
GGTGGCAGCC CAACTAACTG GACACCAGAG GCCGTAGTTA TGTTGGGTAC TACAGCCAGT 
AGAGTAACTG GAGAGTCATG TGAGATACAG GTCCATCCTA TGTTTGAACC ATCTCAAGTT 
TACAGTGACT ATAGACCTGG ACTAGTACTT CCAGAAGAAG CTCACTATTT TATTCCTCAG 
GAAGCAGTGT ATGTAGCTGG GGTACATTAC CAGGCCCGGG TGGCAGAACA GTATGAGGGC 
ATTCCATACA ACTCATCAGT ACTGTCAAGT CCTATGAAAC AGATACCTGA ACAGAAGCCA 
GTACAAGGGG GCCCTACTTC AAGTTCTGTC TTTGAATTTC CATCTGGACA GGCTTTCCTG 
GTAGGACACC TTCAGAATCT AAGATTAGAT TCTGGATTGG GTCCGGGATC TCCCCTCTCT 
AGTATTTCTG CACCTATCAG TACAGATGCT ACACGTTTGA AATTTCACCC TGTCTTTGTT 
CCTCATTCTG CGCCTGCTGT GTTAACTCAT AACAATGAGA GCAGAAGCAA CTGTGTATTT 
GAATTTCATG TTCACACACC AAGCTCCTCT TCAGGAGAAG GAGGTGGAAT TTTACCTCAG 
CGTGTTTACC GAAATCGGCA GGTTGCAGTG GACTTGAATC AAGAAGAACT GCCTCCTCAA 
TCAGTTGGAT TACATGGCTA CTTGCAGCCT GTGACTGAAG AAAAGCATAA TTACCATGCC 
CCAGAATTGA CCGTTTCTGT GGTAGAGCCT ATCGGACAGA ACTGGCCAAT AGGAAGCCCA 
GAATATTCCA GTGATTCCTC ACAAATCACT TCTTCAGACC CCAGTGATTT TCAGTCACCT 
CCCCCTACAG GGGGAGCAGC TGCACCTTTT GGCTCTGACG TCTCAATGCC CTTTATCCAT 
CTGCCTCAGA CAGTGTTACA AGAATCCCCA CTTTTCTTCT GTTTCCCCCA AGGAACCACA 
TCTCAGCAGG TCTTAACTGC CTCATTTTCT TCAGGAGGAT CTGCACTTCA TCCACAGGCA 
CAGGGGCAGA GCCAGGGTCA GCCATCCTCA AGTAGCTTAA CAGGGGTTTC ATCTTCCCAA 
CCCATACAAC ATCCTCAGCA GCAGCAGGGA ATACAGCAGA CAGCCCCTCC TCAACAGACA 
GTGCAGTATT CACTTTCACA GACATCAACC TCCAGTGAGG CCACTACTGC ACAGCCAGTG 
AGTCAGCCTC AAGCTCCACA AGTCTTGCCT CAAGTATCAG CTGGAAAACA GCTTCCAGTT 
TCCCAGCCAG TACCAACTAT CCAAGGCGAA CCTCAGATCC CAGTTGCGAC ACAACCCTCG 
GTTGTTCCAG TCCACTCTGG TGCTCATTTC CTTCCAGTGG GACAGCCGCT CCCTACTCCC 
TTGCTCCCTC AGTACCCTGT CTCTCAGATT CCCATATCAA CTCCTCATGT GTCTACGGCT 
CAGACAGGTT TCTCATCCCT TCCCATCACA ATGGCAGCTG GCATTACTCA GCCTCTGCTC 
ACGTTGGCTT CATCTGCTAC AACAGCTGCG ATCCCGGGGG TATCAACTGT GGTTCCTAGT 
CAGCTTCCAA CCCTTCTGCA GCCTGTGACT CAGCTGCCAA GTCAGGTTCA CCCACAGCTC 
CTACAACCAG CAGTTCAGTC CATGGGAATA CCAGCTAACC TTGGACAAGC TGCTGAGGTT 
CCACTTTCCT CTGGAGATGT TCTGTACCAG GGCTTCCCAC CTCGACTGCC ACCACAGTAC 
CCAGGAGATT CAAATATTGC TCCCTCTTCC AACGTGGCTT CTGTTTGCAT CCATTCTACA 
GTCCTATCCC CTCCCATGCC GACAGAAGTA CTGGCTACAC CTGGGTACTT TCCCACAGTG 
GTGCAGCCTT ATGTGGAATC AAATCTTTTA GTTCCTATGG GTGGTGTAGG AGGACAGGTT 
CAAGTGTCCC AGCCAGGAGG GAGTTTAGCA CAAGCCCCCA CTACATCCTC CCAGCAAGCA 
GTTTTGGAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA 
CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA 
GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA 
ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA 
TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC 
AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA 
GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC 
TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT 
TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG 
GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC 
CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG 
TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA 
ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA 
GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA 
CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG 
GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT 
TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA 
AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC 
ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA 
GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA 
GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC 
ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT 
ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA 
GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA 
GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA 
CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT 
TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC 
TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT 
ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC 
AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT 
TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA 
CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC 
AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG 
GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG 
GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG 
AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT 
TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA 
AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG 
CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC 
CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG 
TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA 
GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA 
CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA 
AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG 
GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT 
CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT 
AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA 
GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG 
AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT 
CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA 
TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT 
CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA 
GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC 
TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA 
GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG 
AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC 
ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC 
GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGCTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519307.1
CDS	674..7840
Translation	MDIKKKDFCSVFVIINSHCCCCPQKDCINEGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011521005.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521005.2

ORF Insert Sequence:

ATGGATATTA AAAAGAAAGA TTTTTGCTCA GTATTTGTCA TCATAAATTC TCATTGCTGC 
TGCTGCCCTC AAAAGGATTG TATAAATGAG GGGGTGAAGC CAGCCAGTTT TGACAAAGTA 
GCAATTCCTG AAGTGAAGGA AATTATTGAA GGATGCATAC GACAAAACAA AGATGAAAGA 
TATTCCATCA AAGACCTTTT GAACCATGCC TTCTTCCAAG AGGAAACAGG AGTACGGGTA 
GAATTAGCAG AAGAAGATGA TGGAGAAAAA ATAGCCATAA AATTATGGCT ACGTATTGAA 
GATATTAAGA AATTAAAGGG AAAATACAAA GATAATGAAG CTATTGAGTT TTCTTTTGAT 
TTAGAGAGAG ATGTCCCAGA AGATGTTGCA CAAGAAATGG TAGAGTCTGG GTATGTCTGT 
GAAGGTGATC ACAAGACCAT GGCTAAAGCT ATCAAAGACA GAGTATCATT AATTAAGAGG 
AAACGAGAGC AGCGGCAGTT GGTACGGGAG GAGCAAGAAA AAAAAAAGCA GGAAGAGAGC 
AGTCTCAAAC AGCAGGTAGA ACAATCCAGT GCTTCCCAGA CAGGAATCAA GCAGCTCCCT 
TCTGCTAGCA CCGGCATACC TACTGCTTCT ACCACTTCAG CTTCAGTTTC TACACAAGTA 
GAACCTGAAG AACCTGAGGC AGATCAACAT CAACAACTAC AGTACCAGCA ACCCAGTATA 
TCTGTGTTAT CTGATGGGAC GGTTGACAGT GGTCAGGGAT CCTCTGTCTT CACAGAATCT 
CGAGTGAGCA GCCAACAGAC AGTTTCATAT GGTTCCCAAC ATGAACAGGC ACATTCTACA 
GGCACAGTCC CAGGGCATAT ACCTTCTACT GTCCAAGCAC AGTCTCAGCC CCATGGGGTA 
TATCCACCCT CAAGTGTGCC TCAGTCCATG GCGCATCCGT GTGGGGGGAC CCCAACATAC 
CCAGAATCAC AGATATTTTT CCCAACTATT CATGAACGTC CAGTTTCTTT TTCACCACCT 
CCCACCTGCC CACCGAAAGT AGCCATTTCC CAGCGGCGTA AGAGCACCTC CTTCCTGGAA 
GCCCAAACTC ACCACTTCCA ACCCCTGCTG AGGACTGTTG GCCAAAGTCT TCTTCCACCT 
GGTGGCAGCC CAACTAACTG GACACCAGAG GCCGTAGTTA TGTTGGGTAC TACAGCCAGT 
AGAGTAACTG GAGAGTCATG TGAGATACAG GTCCATCCTA TGTTTGAACC ATCTCAAGTT 
TACAGTGACT ATAGACCTGG ACTAGTACTT CCAGAAGAAG CTCACTATTT TATTCCTCAG 
GAAGCAGTGT ATGTAGCTGG GGTACATTAC CAGGCCCGGG TGGCAGAACA GTATGAGGGC 
ATTCCATACA ACTCATCAGT ACTGTCAAGT CCTATGAAAC AGATACCTGA ACAGAAGCCA 
GTACAAGGGG GCCCTACTTC AAGTTCTGTC TTTGAATTTC CATCTGGACA GGCTTTCCTG 
GTAGGACACC TTCAGAATCT AAGATTAGAT TCTGGATTGG GTCCGGGATC TCCCCTCTCT 
AGTATTTCTG CACCTATCAG TACAGATGCT ACACGTTTGA AATTTCACCC TGTCTTTGTT 
CCTCATTCTG CGCCTGCTGT GTTAACTCAT AACAATGAGA GCAGAAGCAA CTGTGTATTT 
GAATTTCATG TTCACACACC AAGCTCCTCT TCAGGAGAAG GAGGTGGAAT TTTACCTCAG 
CGTGTTTACC GAAATCGGCA GGTTGCAGTG GACTTGAATC AAGAAGAACT GCCTCCTCAA 
TCAGTTGGAT TACATGGCTA CTTGCAGCCT GTGACTGAAG AAAAGCATAA TTACCATGCC 
CCAGAATTGA CCGTTTCTGT GGTAGAGCCT ATCGGACAGA ACTGGCCAAT AGGAAGCCCA 
GAATATTCCA GTGATTCCTC ACAAATCACT TCTTCAGACC CCAGTGATTT TCAGTCACCT 
CCCCCTACAG GGGGAGCAGC TGCACCTTTT GGCTCTGACG TCTCAATGCC CTTTATCCAT 
CTGCCTCAGA CAGTGTTACA AGAATCCCCA CTTTTCTTCT GTTTCCCCCA AGGAACCACA 
TCTCAGCAGG TCTTAACTGC CTCATTTTCT TCAGGAGGAT CTGCACTTCA TCCACAGGCA 
CAGGGGCAGA GCCAGGGTCA GCCATCCTCA AGTAGCTTAA CAGGGGTTTC ATCTTCCCAA 
CCCATACAAC ATCCTCAGCA GCAGCAGGGA ATACAGCAGA CAGCCCCTCC TCAACAGACA 
GTGCAGTATT CACTTTCACA GACATCAACC TCCAGTGAGG CCACTACTGC ACAGCCAGTG 
AGTCAGCCTC AAGCTCCACA AGTCTTGCCT CAAGTATCAG CTGGAAAACA GCTTCCAGTT 
TCCCAGCCAG TACCAACTAT CCAAGGCGAA CCTCAGATCC CAGTTGCGAC ACAACCCTCG 
GTTGTTCCAG TCCACTCTGG TGCTCATTTC CTTCCAGTGG GACAGCCGCT CCCTACTCCC 
TTGCTCCCTC AGTACCCTGT CTCTCAGATT CCCATATCAA CTCCTCATGT GTCTACGGCT 
CAGACAGGTT TCTCATCCCT TCCCATCACA ATGGCAGCTG GCATTACTCA GCCTCTGCTC 
ACGTTGGCTT CATCTGCTAC AACAGCTGCG ATCCCGGGGG TATCAACTGT GGTTCCTAGT 
CAGCTTCCAA CCCTTCTGCA GCCTGTGACT CAGCTGCCAA GTCAGGTTCA CCCACAGCTC 
CTACAACCAG CAGTTCAGTC CATGGGAATA CCAGCTAACC TTGGACAAGC TGCTGAGGTT 
CCACTTTCCT CTGGAGATGT TCTGTACCAG GGCTTCCCAC CTCGACTGCC ACCACAGTAC 
CCAGGAGATT CAAATATTGC TCCCTCTTCC AACGTGGCTT CTGTTTGCAT CCATTCTACA 
GTCCTATCCC CTCCCATGCC GACAGAAGTA CTGGCTACAC CTGGGTACTT TCCCACAGTG 
GTGCAGCCTT ATGTGGAATC AAATCTTTTA GTTCCTATGG GTGGTGTAGG AGGACAGGTT 
CAAGTGTCCC AGCCAGGAGG GAGTTTAGCA CAAGCCCCCA CTACATCCTC CCAGCAAGCA 
GTTTTGGAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA 
CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA 
GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA 
ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA 
TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC 
AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA 
GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC 
TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT 
TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG 
GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC 
CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG 
TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA 
ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA 
GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA 
CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG 
GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT 
TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA 
AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC 
ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA 
GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA 
GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC 
ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT 
ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA 
GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA 
GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA 
CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT 
TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC 
TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT 
ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC 
AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT 
TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA 
CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC 
AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG 
GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG 
GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG 
AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT 
TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA 
AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG 
CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC 
CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG 
TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA 
GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA 
CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA 
AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG 
GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT 
CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT 
AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA 
GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG 
AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT 
CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA 
TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT 
CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA 
GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC 
TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA 
GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG 
AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC 
ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC 
GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGCTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011521006.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519308.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

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CloneID	OHu71622
Clone ID Related Accession (Same CDS sequence)	XM_011521006.2
Accession Version	XM_011521006.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA 
GTTGCGACAC AACCCTCGGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCAGTGGGA 
CAGCCGCTCC CTACTCCCTT GCTCCCTCAG TACCCTGTCT CTCAGATTCC CATATCAACT 
CCTCATGTGT CTACGGCTCA GACAGGTTTC TCATCCCTTC CCATCACAAT GGCAGCTGGC 
ATTACTCAGC CTCTGCTCAC GTTGGCTTCA TCTGCTACAA CAGCTGCGAT CCCGGGGGTA 
TCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGTTCACC CACAGCTCCT ACAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGTTCC ACTTTCCTCT GGAGATGTTC TGTACCAGGG CTTCCCACCT 
CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT 
GTTTGCATCC ATTCTACAGT CCTATCCCCT CCCATGCCGA CAGAAGTACT GGCTACACCT 
GGGTACTTTC CCACAGTGGT GCAGCCTTAT GTGGAATCAA ATCTTTTAGT TCCTATGGGT 
GGTGTAGGAG GACAGGTTCA AGTGTCCCAG CCAGGAGGGA GTTTAGCACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGT TTTGGAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTGCA 
GAGCCAGTTG CAGTAGCACA GACCCAAGCT ACCCAGCCGA CCACTTTGGC TTCCTCTGTA 
GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA CGTCCCATCT 
TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT 
CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA 
GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA 
TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT 
ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGATCAGGG ATTGGAGAGT 
CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA 
CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT 
CAAGTTGTTC ATTCTGCGGG AAGGCGGTTT ATAGTGAGTC CTGTGCCAGA AAGCCGATTA 
CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACAGCTCAG 
AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT 
GAACTTAGAC GTGCCCAAAT GACAGAAGGA CCCAACACAG CACCTCCAAA CTTTAGTCAT 
ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC 
ACAGCAGCAG CCACAGCACC AGTCCCTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA 
TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC 
AGCACAGGTG TGGTAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA 
CTTTCCAGCG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC 
CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGATCGTTG TTTCTAGTAC AGCACTGTAT 
CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT 
CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA 
TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTGCATTCAG 
CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA 
CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT 
TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTATATTT CTCAGCCTGG TGGGCTGCAT 
CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT 
ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA GCCTGTTGCC 
AATGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC 
AACCAGCCCC ATACTCATTG TCCTGAAGTA GATTCTGATA CACAACCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TGTTCAGTGA ACACAGCTCA 
TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC 
ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TACCACAAGT 
ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG 
GTCACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGAGT GAAACCTGGA 
ACTGCTCCCT CCAAGCCACC TCTAACTAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT 
CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG 
TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATG 
ATCACAGTGA CTTCTGCGGT TGGTCCTGTG TCCATGGCGG CTCCAACAGC AATCACAGAA 
GCAGGAACAC AGCCTCAGAA GGGTGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT 
AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519308.1
CDS	969..8033
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011521006.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521006.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA 
GTTGCGACAC AACCCTCGGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCAGTGGGA 
CAGCCGCTCC CTACTCCCTT GCTCCCTCAG TACCCTGTCT CTCAGATTCC CATATCAACT 
CCTCATGTGT CTACGGCTCA GACAGGTTTC TCATCCCTTC CCATCACAAT GGCAGCTGGC 
ATTACTCAGC CTCTGCTCAC GTTGGCTTCA TCTGCTACAA CAGCTGCGAT CCCGGGGGTA 
TCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGTTCACC CACAGCTCCT ACAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGTTCC ACTTTCCTCT GGAGATGTTC TGTACCAGGG CTTCCCACCT 
CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT 
GTTTGCATCC ATTCTACAGT CCTATCCCCT CCCATGCCGA CAGAAGTACT GGCTACACCT 
GGGTACTTTC CCACAGTGGT GCAGCCTTAT GTGGAATCAA ATCTTTTAGT TCCTATGGGT 
GGTGTAGGAG GACAGGTTCA AGTGTCCCAG CCAGGAGGGA GTTTAGCACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGT TTTGGAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTGCA 
GAGCCAGTTG CAGTAGCACA GACCCAAGCT ACCCAGCCGA CCACTTTGGC TTCCTCTGTA 
GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA CGTCCCATCT 
TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT 
CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA 
GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA 
TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT 
ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGATCAGGG ATTGGAGAGT 
CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA 
CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT 
CAAGTTGTTC ATTCTGCGGG AAGGCGGTTT ATAGTGAGTC CTGTGCCAGA AAGCCGATTA 
CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACAGCTCAG 
AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT 
GAACTTAGAC GTGCCCAAAT GACAGAAGGA CCCAACACAG CACCTCCAAA CTTTAGTCAT 
ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC 
ACAGCAGCAG CCACAGCACC AGTCCCTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA 
TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC 
AGCACAGGTG TGGTAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA 
CTTTCCAGCG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC 
CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGATCGTTG TTTCTAGTAC AGCACTGTAT 
CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT 
CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA 
TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTGCATTCAG 
CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA 
CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT 
TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTATATTT CTCAGCCTGG TGGGCTGCAT 
CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT 
ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA GCCTGTTGCC 
AATGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC 
AACCAGCCCC ATACTCATTG TCCTGAAGTA GATTCTGATA CACAACCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TGTTCAGTGA ACACAGCTCA 
TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC 
ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TACCACAAGT 
ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG 
GTCACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGAGT GAAACCTGGA 
ACTGCTCCCT CCAAGCCACC TCTAACTAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT 
CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG 
TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATG 
ATCACAGTGA CTTCTGCGGT TGGTCCTGTG TCCATGGCGG CTCCAACAGC AATCACAGAA 
GCAGGAACAC AGCCTCAGAA GGGTGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT 
AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011521007.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519309.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

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CloneID	OHu71623
Clone ID Related Accession (Same CDS sequence)	XM_011521007.2
Accession Version	XM_011521007.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521007.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC 
CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT 
ACTGCACAGC CAGTGAGTCA GCCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA 
AAACAGCTTC CAGTTTCCCA GCCAGTACCA ACTATCCAAG GCGAACCTCA GATCCCAGTT 
GCGACACAAC CCTCGGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC AGTGGGACAG 
CCGCTCCCTA CTCCCTTGCT CCCTCAGTAC CCTGTCTCTC AGATTCCCAT ATCAACTCCT 
CATGTGTCTA CGGCTCAGAC AGGTTTCTCA TCCCTTCCCA TCACAATGGC AGCTGGCATT 
ACTCAGCCTC TGCTCACGTT GGCTTCATCT GCTACAACAG CTGCGATCCC GGGGGTATCA 
ACTGTGGTTC CTAGTCAGCT TCCAACCCTT CTGCAGCCTG TGACTCAGCT GCCAAGTCAG 
GTTCACCCAC AGCTCCTACA ACCAGCAGTT CAGTCCATGG GAATACCAGC TAACCTTGGA 
CAAGCTGCTG AGGTTCCACT TTCCTCTGGA GATGTTCTGT ACCAGGGCTT CCCACCTCGA 
CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCCT CTTCCAACGT GGCTTCTGTT 
TGCATCCATT CTACAGTCCT ATCCCCTCCC ATGCCGACAG AAGTACTGGC TACACCTGGG 
TACTTTCCCA CAGTGGTGCA GCCTTATGTG GAATCAAATC TTTTAGTTCC TATGGGTGGT 
GTAGGAGGAC AGGTTCAAGT GTCCCAGCCA GGAGGGAGTT TAGCACAAGC CCCCACTACA 
TCCTCCCAGC AAGCAGTTTT GGAGAGTACT CAGGGAGTCT CTCAGGTTGC TCCTGCAGAG 
CCAGTTGCAG TAGCACAGAC CCAAGCTACC CAGCCGACCA CTTTGGCTTC CTCTGTAGAC 
AGTGCACATT CAGATGTTGC TTCAGGTATG AGTGATGGCA ATGAGAACGT CCCATCTTCC 
AGTGGAAGGC ATGAAGGAAG AACTACAAAA CGGCATTACC GAAAATCTGT AAGGAGTCGC 
TCTCGACATG AAAAAACTTC ACGCCCAAAA TTAAGAATTT TGAATGTTTC AAATAAAGGA 
GACCGAGTAG TAGAATGTCA ATTAGAGACT CATAATAGGA AAATGGTTAC ATTCAAATTT 
GACCTAGATG GTGACAACCC CGAGGAGATA GCAACAATTA TGGTGAACAA TGACTTTATT 
CTAGCAATAG AGAGAGAGTC GTTTGTGGAT CAAGTGCGAG AAATTATTGA AAAAGCTGAT 
GAAATGCTCA GTGAGGATGT CAGTGTGGAA CCAGAGGGTG ATCAGGGATT GGAGAGTCTA 
CAAGGAAAGG ATGACTATGG CTTTTCAGGT TCTCAGAAAT TGGAAGGAGA GTTCAAACAA 
CCAATTCCTG CGTCTTCCAT GCCACAGCAA ATAGGCATTC CTACCAGTTC TTTAACTCAA 
GTTGTTCATT CTGCGGGAAG GCGGTTTATA GTGAGTCCTG TGCCAGAAAG CCGATTACGA 
GAATCAAAAG TTTTCCCCAG TGAAATAACA GATACAGTTG CTGCCTCTAC AGCTCAGAGC 
CCTGGAATGA ACTTGTCTCA CTCTGCATCA TCCCTTAGTC TACAACAGGC CTTTTCTGAA 
CTTAGACGTG CCCAAATGAC AGAAGGACCC AACACAGCAC CTCCAAACTT TAGTCATACA 
GGACCAACAT TTCCAGTAGT ACCTCCTTTC TTAAGTAGCA TTGCTGGAGT CCCAACCACA 
GCAGCAGCCA CAGCACCAGT CCCTGCAACA AGCAGCCCTC CTAATGACAT TTCCACATCA 
GTAATTCAGT CTGAGGTTAC AGTGCCCACT GAAGAGGGGA TTGCTGGAGT TGCCACCAGC 
ACAGGTGTGG TAACTTCAGG TGGTCTCCCC ATACCACCTG TGTCTGAATC ACCAGTACTT 
TCCAGCGTAG TTTCAAGTAT CACAATACCT GCAGTTGTCT CAATATCTAC TACATCCCCG 
TCACTTCAAG TCCCCACATC CACATCTGAG ATCGTTGTTT CTAGTACAGC ACTGTATCCT 
TCAGTAACAG TTTCAGCAAC TTCAGCCTCT GCAGGGGGCA GTACTGCTAC CCCAGGTCCT 
AAGCCTCCAG CTGTAGTATC TCAGCAGGCA GCAGGCAGCA CTACTGTGGG AGCCACATTA 
ACATCAGTTT CTACCACCAC TTCATTCCCA AGCACAGCTT CACAGCTGTG CATTCAGCTT 
AGCAGCAGTA CTTCTACTCC TACTTTAGCT GAAACCGTGG TAGTTAGCGC ACACTCACTA 
GATAAGACAT CTCATAGCAG TACAACTGGA TTGGCTTTCT CCCTCTCTGC ACCATCTTCC 
TCTTCCTCTC CTGGAGCAGG AGTGTCTAGT TATATTTCTC AGCCTGGTGG GCTGCATCCT 
TTGGTCATTC CATCAGTGAT AGCTTCTACT CCTATTCTTC CCCAAGCAGC AGGACCTACT 
TCTACACCTT TATTACCCCA AGTACCTAGT ATCCCACCCT TGGTACAGCC TGTTGCCAAT 
GTGCCTGCTG TACAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT GCTTCCCAAC 
CAGCCCCATA CTCATTGTCC TGAAGTAGAT TCTGATACAC AACCCAAAGC TCCTGGAATT 
GATGACATAA AGACTCTAGA AGAAAAGCTG CGGTCTCTGT TCAGTGAACA CAGCTCATCT 
GGAGCTCAGC ATGCCTCTGT CTCACTGGAG ACCTCACTAG TCATAGAGAG CACTGTCACA 
CCAGGCATCC CAACTACTGC TGTTGCACCA AGCAAACTCC TGACTTCTAC CACAAGTACT 
TGCTTACCAC CAACCAATTT ACCACTAGGA ACAGTTGCTT TGCCAGTTAC ACCAGTGGTC 
ACACCTGGGC AAGTTTCTAC CCCAGTCAGC ACTACTACAT CAGGAGTGAA ACCTGGAACT 
GCTCCCTCCA AGCCACCTCT AACTAAGGCT CCGGTGCTGC CAGTGGGTAC TGAACTTCCA 
GCAGGTACTC TACCCAGCGA GCAGCTGCCA CCTTTTCCAG GACCTTCTCT AACCCAGTCC 
CAGCAACCTC TAGAGGATCT TGATGCTCAA TTGAGAAGAA CACTTAGTCC AGAGATGATC 
ACAGTGACTT CTGCGGTTGG TCCTGTGTCC ATGGCGGCTC CAACAGCAAT CACAGAAGCA 
GGAACACAGC CTCAGAAGGG TGTTTCTCAA GTCAAAGAAG GCCCTGTCCT AGCAACTAGT 
TCAGGAGCTG GTGTTTTTAA GATGGGACGA TTTCAGGTTT CTGTTGCAGC AGACGGTGCC 
CAGAAAGAGG GTAAAAATAA GTCAGAAGAT GCAAAGTCTG TTCATTTTGA ATCCAGCACC 
TCAGAGTCCT CAGTGCTATC AAGTAGTAGT CCAGAGAGTA CCTTGGTGAA ACCAGAGCCG 
AATGGCATAA CCATCCCTGG TATCTCTTCA GATGTGCCAG AGAGTGCCCA CAAAACTACT 
GCCTCAGAGG CAAAGTCAGA CACTGGGCAG CCTACCAAGG TTGGACGTTT TCAGGTGACA 
ACTACAGCAA ACAAAGTGGG TCGTTTCTCT GTATCAAAAA CTGAGGACAA GATCACTGAC 
ACAAAGAAAG AAGGACCAGT GGCATCTCCT CCTTTTATGG ATTTGGAACA AGCTGTTCTT 
CCTGCTGTGA TACCAAAGAA AGAGAAGCCT GAACTGTCAG AGCCTTCACA TCTAAATGGG 
CCGTCTTCTG ACCCGGAGGC CGCTTTTTTA AGTAGGGATG TGGATGATGG TTCCGGTAGT 
CCACACTCGC CCCATCAGCT GAGCTCAAAG AGCCTTCCTA GCCAGAATCT AAGTCAAAGC 
CTTAGTAATT CATTTAACTC CTCTTACATG AGTAGCGACA ATGAGTCAGA TATCGAAGAT 
GAAGACTTAA AGTTAGAGCT GCGACGACTA CGAGATAAAC ATCTCAAAGA GATTCAGGAC 
CTGCAGAGTC GCCAGAAGCA TGAAATTGAA TCTTTGTATA CCAAACTGGG CAAGGTGCCC 
CCTGCTGTTA TTATTCCCCC AGCTGCTCCC CTTTCAGGGA GAAGACGACG ACCCACTAAA 
AGCAAAGGCA GCAAATCTAG TCGAAGCAGT TCCTTGGGGA ATAAAAGCCC CCAGCTTTCA 
GGTAACCTGT CTGGTCAGAG TGCAGCTTCA GTCTTGCACC CCCAGCAGAC CCTCCACCCT 
CCTGGCAACA TCCCAGAGTC CGGGCAGAAT CAGCTGTTAC AGCCCCTTAA GCCATCTCCC 
TCCAGTGACA ACCTCTATTC AGCCTTCACC AGTGATGGTG CCATTTCAGT ACCAAGCCTT 
TCTGCTCCAG GTCAAGGAAC CAGCAGCACA AACACTGTTG GGGCAACAGT GAACAGCCAA 
GCCGCCCAAG CTCAGCCTCC TGCCATGACG TCCAGCAGGA AGGGCACATT CACAGATGAC 
TTGCACAAGT TGGTAGACAA TTGGGCCCGA GATGCCATGA ATCTCTCAGG CAGGAGAGGA 
AGCAAAGGGC ACATGAATTA CGAGGGCCCT GGAATGGCAA GGAAGTTCTC TGCACCTGGG 
CAACTGTGCA TCTCCATGAC CTCGAACCTG GGTGGCTCTG CCCCCATCTC TGCAGCATCA 
GCTACCTCTC TAGGTCACTT CACCAAGTCT ATGTGCCCCC CACAGCAGTA TGGCTTTCCA 
GCTACCCCAT TTGGCGCTCA ATGGAGTGGG ACGGGTGGCC CAGCACCACA GCCACTTGGC 
CAGTTCCAAC CTGTGGGAAC TGCCTCCTTG CAGAATTTCA ACATCAGCAA TTTGCAGAAA 
TCCATCAGCA ACCCCCCAGG CTCCAACCTG CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519309.1
CDS	969..8030
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011521007.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521007.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC 
CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT 
ACTGCACAGC CAGTGAGTCA GCCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA 
AAACAGCTTC CAGTTTCCCA GCCAGTACCA ACTATCCAAG GCGAACCTCA GATCCCAGTT 
GCGACACAAC CCTCGGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC AGTGGGACAG 
CCGCTCCCTA CTCCCTTGCT CCCTCAGTAC CCTGTCTCTC AGATTCCCAT ATCAACTCCT 
CATGTGTCTA CGGCTCAGAC AGGTTTCTCA TCCCTTCCCA TCACAATGGC AGCTGGCATT 
ACTCAGCCTC TGCTCACGTT GGCTTCATCT GCTACAACAG CTGCGATCCC GGGGGTATCA 
ACTGTGGTTC CTAGTCAGCT TCCAACCCTT CTGCAGCCTG TGACTCAGCT GCCAAGTCAG 
GTTCACCCAC AGCTCCTACA ACCAGCAGTT CAGTCCATGG GAATACCAGC TAACCTTGGA 
CAAGCTGCTG AGGTTCCACT TTCCTCTGGA GATGTTCTGT ACCAGGGCTT CCCACCTCGA 
CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCCT CTTCCAACGT GGCTTCTGTT 
TGCATCCATT CTACAGTCCT ATCCCCTCCC ATGCCGACAG AAGTACTGGC TACACCTGGG 
TACTTTCCCA CAGTGGTGCA GCCTTATGTG GAATCAAATC TTTTAGTTCC TATGGGTGGT 
GTAGGAGGAC AGGTTCAAGT GTCCCAGCCA GGAGGGAGTT TAGCACAAGC CCCCACTACA 
TCCTCCCAGC AAGCAGTTTT GGAGAGTACT CAGGGAGTCT CTCAGGTTGC TCCTGCAGAG 
CCAGTTGCAG TAGCACAGAC CCAAGCTACC CAGCCGACCA CTTTGGCTTC CTCTGTAGAC 
AGTGCACATT CAGATGTTGC TTCAGGTATG AGTGATGGCA ATGAGAACGT CCCATCTTCC 
AGTGGAAGGC ATGAAGGAAG AACTACAAAA CGGCATTACC GAAAATCTGT AAGGAGTCGC 
TCTCGACATG AAAAAACTTC ACGCCCAAAA TTAAGAATTT TGAATGTTTC AAATAAAGGA 
GACCGAGTAG TAGAATGTCA ATTAGAGACT CATAATAGGA AAATGGTTAC ATTCAAATTT 
GACCTAGATG GTGACAACCC CGAGGAGATA GCAACAATTA TGGTGAACAA TGACTTTATT 
CTAGCAATAG AGAGAGAGTC GTTTGTGGAT CAAGTGCGAG AAATTATTGA AAAAGCTGAT 
GAAATGCTCA GTGAGGATGT CAGTGTGGAA CCAGAGGGTG ATCAGGGATT GGAGAGTCTA 
CAAGGAAAGG ATGACTATGG CTTTTCAGGT TCTCAGAAAT TGGAAGGAGA GTTCAAACAA 
CCAATTCCTG CGTCTTCCAT GCCACAGCAA ATAGGCATTC CTACCAGTTC TTTAACTCAA 
GTTGTTCATT CTGCGGGAAG GCGGTTTATA GTGAGTCCTG TGCCAGAAAG CCGATTACGA 
GAATCAAAAG TTTTCCCCAG TGAAATAACA GATACAGTTG CTGCCTCTAC AGCTCAGAGC 
CCTGGAATGA ACTTGTCTCA CTCTGCATCA TCCCTTAGTC TACAACAGGC CTTTTCTGAA 
CTTAGACGTG CCCAAATGAC AGAAGGACCC AACACAGCAC CTCCAAACTT TAGTCATACA 
GGACCAACAT TTCCAGTAGT ACCTCCTTTC TTAAGTAGCA TTGCTGGAGT CCCAACCACA 
GCAGCAGCCA CAGCACCAGT CCCTGCAACA AGCAGCCCTC CTAATGACAT TTCCACATCA 
GTAATTCAGT CTGAGGTTAC AGTGCCCACT GAAGAGGGGA TTGCTGGAGT TGCCACCAGC 
ACAGGTGTGG TAACTTCAGG TGGTCTCCCC ATACCACCTG TGTCTGAATC ACCAGTACTT 
TCCAGCGTAG TTTCAAGTAT CACAATACCT GCAGTTGTCT CAATATCTAC TACATCCCCG 
TCACTTCAAG TCCCCACATC CACATCTGAG ATCGTTGTTT CTAGTACAGC ACTGTATCCT 
TCAGTAACAG TTTCAGCAAC TTCAGCCTCT GCAGGGGGCA GTACTGCTAC CCCAGGTCCT 
AAGCCTCCAG CTGTAGTATC TCAGCAGGCA GCAGGCAGCA CTACTGTGGG AGCCACATTA 
ACATCAGTTT CTACCACCAC TTCATTCCCA AGCACAGCTT CACAGCTGTG CATTCAGCTT 
AGCAGCAGTA CTTCTACTCC TACTTTAGCT GAAACCGTGG TAGTTAGCGC ACACTCACTA 
GATAAGACAT CTCATAGCAG TACAACTGGA TTGGCTTTCT CCCTCTCTGC ACCATCTTCC 
TCTTCCTCTC CTGGAGCAGG AGTGTCTAGT TATATTTCTC AGCCTGGTGG GCTGCATCCT 
TTGGTCATTC CATCAGTGAT AGCTTCTACT CCTATTCTTC CCCAAGCAGC AGGACCTACT 
TCTACACCTT TATTACCCCA AGTACCTAGT ATCCCACCCT TGGTACAGCC TGTTGCCAAT 
GTGCCTGCTG TACAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT GCTTCCCAAC 
CAGCCCCATA CTCATTGTCC TGAAGTAGAT TCTGATACAC AACCCAAAGC TCCTGGAATT 
GATGACATAA AGACTCTAGA AGAAAAGCTG CGGTCTCTGT TCAGTGAACA CAGCTCATCT 
GGAGCTCAGC ATGCCTCTGT CTCACTGGAG ACCTCACTAG TCATAGAGAG CACTGTCACA 
CCAGGCATCC CAACTACTGC TGTTGCACCA AGCAAACTCC TGACTTCTAC CACAAGTACT 
TGCTTACCAC CAACCAATTT ACCACTAGGA ACAGTTGCTT TGCCAGTTAC ACCAGTGGTC 
ACACCTGGGC AAGTTTCTAC CCCAGTCAGC ACTACTACAT CAGGAGTGAA ACCTGGAACT 
GCTCCCTCCA AGCCACCTCT AACTAAGGCT CCGGTGCTGC CAGTGGGTAC TGAACTTCCA 
GCAGGTACTC TACCCAGCGA GCAGCTGCCA CCTTTTCCAG GACCTTCTCT AACCCAGTCC 
CAGCAACCTC TAGAGGATCT TGATGCTCAA TTGAGAAGAA CACTTAGTCC AGAGATGATC 
ACAGTGACTT CTGCGGTTGG TCCTGTGTCC ATGGCGGCTC CAACAGCAAT CACAGAAGCA 
GGAACACAGC CTCAGAAGGG TGTTTCTCAA GTCAAAGAAG GCCCTGTCCT AGCAACTAGT 
TCAGGAGCTG GTGTTTTTAA GATGGGACGA TTTCAGGTTT CTGTTGCAGC AGACGGTGCC 
CAGAAAGAGG GTAAAAATAA GTCAGAAGAT GCAAAGTCTG TTCATTTTGA ATCCAGCACC 
TCAGAGTCCT CAGTGCTATC AAGTAGTAGT CCAGAGAGTA CCTTGGTGAA ACCAGAGCCG 
AATGGCATAA CCATCCCTGG TATCTCTTCA GATGTGCCAG AGAGTGCCCA CAAAACTACT 
GCCTCAGAGG CAAAGTCAGA CACTGGGCAG CCTACCAAGG TTGGACGTTT TCAGGTGACA 
ACTACAGCAA ACAAAGTGGG TCGTTTCTCT GTATCAAAAA CTGAGGACAA GATCACTGAC 
ACAAAGAAAG AAGGACCAGT GGCATCTCCT CCTTTTATGG ATTTGGAACA AGCTGTTCTT 
CCTGCTGTGA TACCAAAGAA AGAGAAGCCT GAACTGTCAG AGCCTTCACA TCTAAATGGG 
CCGTCTTCTG ACCCGGAGGC CGCTTTTTTA AGTAGGGATG TGGATGATGG TTCCGGTAGT 
CCACACTCGC CCCATCAGCT GAGCTCAAAG AGCCTTCCTA GCCAGAATCT AAGTCAAAGC 
CTTAGTAATT CATTTAACTC CTCTTACATG AGTAGCGACA ATGAGTCAGA TATCGAAGAT 
GAAGACTTAA AGTTAGAGCT GCGACGACTA CGAGATAAAC ATCTCAAAGA GATTCAGGAC 
CTGCAGAGTC GCCAGAAGCA TGAAATTGAA TCTTTGTATA CCAAACTGGG CAAGGTGCCC 
CCTGCTGTTA TTATTCCCCC AGCTGCTCCC CTTTCAGGGA GAAGACGACG ACCCACTAAA 
AGCAAAGGCA GCAAATCTAG TCGAAGCAGT TCCTTGGGGA ATAAAAGCCC CCAGCTTTCA 
GGTAACCTGT CTGGTCAGAG TGCAGCTTCA GTCTTGCACC CCCAGCAGAC CCTCCACCCT 
CCTGGCAACA TCCCAGAGTC CGGGCAGAAT CAGCTGTTAC AGCCCCTTAA GCCATCTCCC 
TCCAGTGACA ACCTCTATTC AGCCTTCACC AGTGATGGTG CCATTTCAGT ACCAAGCCTT 
TCTGCTCCAG GTCAAGGAAC CAGCAGCACA AACACTGTTG GGGCAACAGT GAACAGCCAA 
GCCGCCCAAG CTCAGCCTCC TGCCATGACG TCCAGCAGGA AGGGCACATT CACAGATGAC 
TTGCACAAGT TGGTAGACAA TTGGGCCCGA GATGCCATGA ATCTCTCAGG CAGGAGAGGA 
AGCAAAGGGC ACATGAATTA CGAGGGCCCT GGAATGGCAA GGAAGTTCTC TGCACCTGGG 
CAACTGTGCA TCTCCATGAC CTCGAACCTG GGTGGCTCTG CCCCCATCTC TGCAGCATCA 
GCTACCTCTC TAGGTCACTT CACCAAGTCT ATGTGCCCCC CACAGCAGTA TGGCTTTCCA 
GCTACCCCAT TTGGCGCTCA ATGGAGTGGG ACGGGTGGCC CAGCACCACA GCCACTTGGC 
CAGTTCCAAC CTGTGGGAAC TGCCTCCTTG CAGAATTTCA ACATCAGCAA TTTGCAGAAA 
TCCATCAGCA ACCCCCCAGG CTCCAACCTG CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011521008.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519310.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.

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CloneID	OHu71624
Clone ID Related Accession (Same CDS sequence)	XM_011521008.2
Accession Version	XM_011521008.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA 
CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA 
GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA 
ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA 
TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC 
AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA 
GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC 
TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT 
TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG 
GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC 
CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG 
TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA 
ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA 
GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA 
CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG 
GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT 
TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA 
AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC 
ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA 
GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA 
GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC 
ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT 
ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA 
GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA 
GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA 
CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT 
TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC 
TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT 
ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC 
AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT 
TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA 
CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC 
AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG 
GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG 
GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG 
AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT 
TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA 
AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG 
CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC 
CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG 
TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA 
GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA 
CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA 
AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG 
GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT 
CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT 
AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA 
GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG 
AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT 
CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA 
TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT 
CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA 
GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC 
TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA 
GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG 
AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC 
ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC 
GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGCTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519310.1
CDS	969..7295
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011521008.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521008.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA 
CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA 
GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA 
ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA 
TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC 
AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA 
GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC 
TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT 
TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG 
GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC 
CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG 
TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA 
ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA 
GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA 
CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG 
GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT 
TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA 
AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC 
ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA 
GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA 
GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC 
ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT 
ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA 
GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA 
GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA 
CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT 
TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC 
TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT 
ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC 
AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT 
TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA 
CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC 
AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG 
GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG 
GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG 
AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT 
TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA 
AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG 
CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC 
CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG 
TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA 
GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA 
CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA 
AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG 
GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT 
CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT 
AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA 
GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG 
AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT 
CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA 
TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT 
CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA 
GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC 
TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA 
GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG 
AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC 
ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC 
GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGCTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011521009.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519311.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA.

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CloneID	OHu71625
Clone ID Related Accession (Same CDS sequence)	XM_011521009.2
Accession Version	XM_011521009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC 
CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT 
ACTGCACAGC CAGTGAGTCA GCCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA 
AAACAGAGTA CTCAGGGAGT CTCTCAGGTT GCTCCTGCAG AGCCAGTTGC AGTAGCACAG 
ACCCAAGCTA CCCAGCCGAC CACTTTGGCT TCCTCTGTAG ACAGTGCACA TTCAGATGTT 
GCTTCAGGTA TGAGTGATGG CAATGAGAAC GTCCCATCTT CCAGTGGAAG GCATGAAGGA 
AGAACTACAA AACGGCATTA CCGAAAATCT GTAAGGAGTC GCTCTCGACA TGAAAAAACT 
TCACGCCCAA AATTAAGAAT TTTGAATGTT TCAAATAAAG GAGACCGAGT AGTAGAATGT 
CAATTAGAGA CTCATAATAG GAAAATGGTT ACATTCAAAT TTGACCTAGA TGGTGACAAC 
CCCGAGGAGA TAGCAACAAT TATGGTGAAC AATGACTTTA TTCTAGCAAT AGAGAGAGAG 
TCGTTTGTGG ATCAAGTGCG AGAAATTATT GAAAAAGCTG ATGAAATGCT CAGTGAGGAT 
GTCAGTGTGG AACCAGAGGG TGATCAGGGA TTGGAGAGTC TACAAGGAAA GGATGACTAT 
GGCTTTTCAG GTTCTCAGAA ATTGGAAGGA GAGTTCAAAC AACCAATTCC TGCGTCTTCC 
ATGCCACAGC AAATAGGCAT TCCTACCAGT TCTTTAACTC AAGTTGTTCA TTCTGCGGGA 
AGGCGGTTTA TAGTGAGTCC TGTGCCAGAA AGCCGATTAC GAGAATCAAA AGTTTTCCCC 
AGTGAAATAA CAGATACAGT TGCTGCCTCT ACAGCTCAGA GCCCTGGAAT GAACTTGTCT 
CACTCTGCAT CATCCCTTAG TCTACAACAG GCCTTTTCTG AACTTAGACG TGCCCAAATG 
ACAGAAGGAC CCAACACAGC ACCTCCAAAC TTTAGTCATA CAGGACCAAC ATTTCCAGTA 
GTACCTCCTT TCTTAAGTAG CATTGCTGGA GTCCCAACCA CAGCAGCAGC CACAGCACCA 
GTCCCTGCAA CAAGCAGCCC TCCTAATGAC ATTTCCACAT CAGTAATTCA GTCTGAGGTT 
ACAGTGCCCA CTGAAGAGGG GATTGCTGGA GTTGCCACCA GCACAGGTGT GGTAACTTCA 
GGTGGTCTCC CCATACCACC TGTGTCTGAA TCACCAGTAC TTTCCAGCGT AGTTTCAAGT 
ATCACAATAC CTGCAGTTGT CTCAATATCT ACTACATCCC CGTCACTTCA AGTCCCCACA 
TCCACATCTG AGATCGTTGT TTCTAGTACA GCACTGTATC CTTCAGTAAC AGTTTCAGCA 
ACTTCAGCCT CTGCAGGGGG CAGTACTGCT ACCCCAGGTC CTAAGCCTCC AGCTGTAGTA 
TCTCAGCAGG CAGCAGGCAG CACTACTGTG GGAGCCACAT TAACATCAGT TTCTACCACC 
ACTTCATTCC CAAGCACAGC TTCACAGCTG TGCATTCAGC TTAGCAGCAG TACTTCTACT 
CCTACTTTAG CTGAAACCGT GGTAGTTAGC GCACACTCAC TAGATAAGAC ATCTCATAGC 
AGTACAACTG GATTGGCTTT CTCCCTCTCT GCACCATCTT CCTCTTCCTC TCCTGGAGCA 
GGAGTGTCTA GTTATATTTC TCAGCCTGGT GGGCTGCATC CTTTGGTCAT TCCATCAGTG 
ATAGCTTCTA CTCCTATTCT TCCCCAAGCA GCAGGACCTA CTTCTACACC TTTATTACCC 
CAAGTACCTA GTATCCCACC CTTGGTACAG CCTGTTGCCA ATGTGCCTGC TGTACAGCAG 
ACACTAATTC ATAGTCAGCC TCAACCAGCT TTGCTTCCCA ACCAGCCCCA TACTCATTGT 
CCTGAAGTAG ATTCTGATAC ACAACCCAAA GCTCCTGGAA TTGATGACAT AAAGACTCTA 
GAAGAAAAGC TGCGGTCTCT GTTCAGTGAA CACAGCTCAT CTGGAGCTCA GCATGCCTCT 
GTCTCACTGG AGACCTCACT AGTCATAGAG AGCACTGTCA CACCAGGCAT CCCAACTACT 
GCTGTTGCAC CAAGCAAACT CCTGACTTCT ACCACAAGTA CTTGCTTACC ACCAACCAAT 
TTACCACTAG GAACAGTTGC TTTGCCAGTT ACACCAGTGG TCACACCTGG GCAAGTTTCT 
ACCCCAGTCA GCACTACTAC ATCAGGAGTG AAACCTGGAA CTGCTCCCTC CAAGCCACCT 
CTAACTAAGG CTCCGGTGCT GCCAGTGGGT ACTGAACTTC CAGCAGGTAC TCTACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCT CTAACCCAGT CCCAGCAACC TCTAGAGGAT 
CTTGATGCTC AATTGAGAAG AACACTTAGT CCAGAGATGA TCACAGTGAC TTCTGCGGTT 
GGTCCTGTGT CCATGGCGGC TCCAACAGCA ATCACAGAAG CAGGAACACA GCCTCAGAAG 
GGTGTTTCTC AAGTCAAAGA AGGCCCTGTC CTAGCAACTA GTTCAGGAGC TGGTGTTTTT 
AAGATGGGAC GATTTCAGGT TTCTGTTGCA GCAGACGGTG CCCAGAAAGA GGGTAAAAAT 
AAGTCAGAAG ATGCAAAGTC TGTTCATTTT GAATCCAGCA CCTCAGAGTC CTCAGTGCTA 
TCAAGTAGTA GTCCAGAGAG TACCTTGGTG AAACCAGAGC CGAATGGCAT AACCATCCCT 
GGTATCTCTT CAGATGTGCC AGAGAGTGCC CACAAAACTA CTGCCTCAGA GGCAAAGTCA 
GACACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTG 
GGTCGTTTCT CTGTATCAAA AACTGAGGAC AAGATCACTG ACACAAAGAA AGAAGGACCA 
GTGGCATCTC CTCCTTTTAT GGATTTGGAA CAAGCTGTTC TTCCTGCTGT GATACCAAAG 
AAAGAGAAGC CTGAACTGTC AGAGCCTTCA CATCTAAATG GGCCGTCTTC TGACCCGGAG 
GCCGCTTTTT TAAGTAGGGA TGTGGATGAT GGTTCCGGTA GTCCACACTC GCCCCATCAG 
CTGAGCTCAA AGAGCCTTCC TAGCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTTAAC 
TCCTCTTACA TGAGTAGCGA CAATGAGTCA GATATCGAAG ATGAAGACTT AAAGTTAGAG 
CTGCGACGAC TACGAGATAA ACATCTCAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTGTA TACCAAACTG GGCAAGGTGC CCCCTGCTGT TATTATTCCC 
CCAGCTGCTC CCCTTTCAGG GAGAAGACGA CGACCCACTA AAAGCAAAGG CAGCAAATCT 
AGTCGAAGCA GTTCCTTGGG GAATAAAAGC CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCAGCTT CAGTCTTGCA CCCCCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCAGAG 
TCCGGGCAGA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAT 
TCAGCCTTCA CCAGTGATGG TGCCATTTCA GTACCAAGCC TTTCTGCTCC AGGTCAAGGA 
ACCAGCAGCA CAAACACTGT TGGGGCAACA GTGAACAGCC AAGCCGCCCA AGCTCAGCCT 
CCTGCCATGA CGTCCAGCAG GAAGGGCACA TTCACAGATG ACTTGCACAA GTTGGTAGAC 
AATTGGGCCC GAGATGCCAT GAATCTCTCA GGCAGGAGAG GAAGCAAAGG GCACATGAAT 
TACGAGGGCC CTGGAATGGC AAGGAAGTTC TCTGCACCTG GGCAACTGTG CATCTCCATG 
ACCTCGAACC TGGGTGGCTC TGCCCCCATC TCTGCAGCAT CAGCTACCTC TCTAGGTCAC 
TTCACCAAGT CTATGTGCCC CCCACAGCAG TATGGCTTTC CAGCTACCCC ATTTGGCGCT 
CAATGGAGTG GGACGGGTGG CCCAGCACCA CAGCCACTTG GCCAGTTCCA ACCTGTGGGA 
ACTGCCTCCT TGCAGAATTT CAACATCAGC AATTTGCAGA AATCCATCAG CAACCCCCCA 
GGCTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519311.1
CDS	969..7292
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011521009.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521009.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC 
CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT 
ACTGCACAGC CAGTGAGTCA GCCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA 
AAACAGAGTA CTCAGGGAGT CTCTCAGGTT GCTCCTGCAG AGCCAGTTGC AGTAGCACAG 
ACCCAAGCTA CCCAGCCGAC CACTTTGGCT TCCTCTGTAG ACAGTGCACA TTCAGATGTT 
GCTTCAGGTA TGAGTGATGG CAATGAGAAC GTCCCATCTT CCAGTGGAAG GCATGAAGGA 
AGAACTACAA AACGGCATTA CCGAAAATCT GTAAGGAGTC GCTCTCGACA TGAAAAAACT 
TCACGCCCAA AATTAAGAAT TTTGAATGTT TCAAATAAAG GAGACCGAGT AGTAGAATGT 
CAATTAGAGA CTCATAATAG GAAAATGGTT ACATTCAAAT TTGACCTAGA TGGTGACAAC 
CCCGAGGAGA TAGCAACAAT TATGGTGAAC AATGACTTTA TTCTAGCAAT AGAGAGAGAG 
TCGTTTGTGG ATCAAGTGCG AGAAATTATT GAAAAAGCTG ATGAAATGCT CAGTGAGGAT 
GTCAGTGTGG AACCAGAGGG TGATCAGGGA TTGGAGAGTC TACAAGGAAA GGATGACTAT 
GGCTTTTCAG GTTCTCAGAA ATTGGAAGGA GAGTTCAAAC AACCAATTCC TGCGTCTTCC 
ATGCCACAGC AAATAGGCAT TCCTACCAGT TCTTTAACTC AAGTTGTTCA TTCTGCGGGA 
AGGCGGTTTA TAGTGAGTCC TGTGCCAGAA AGCCGATTAC GAGAATCAAA AGTTTTCCCC 
AGTGAAATAA CAGATACAGT TGCTGCCTCT ACAGCTCAGA GCCCTGGAAT GAACTTGTCT 
CACTCTGCAT CATCCCTTAG TCTACAACAG GCCTTTTCTG AACTTAGACG TGCCCAAATG 
ACAGAAGGAC CCAACACAGC ACCTCCAAAC TTTAGTCATA CAGGACCAAC ATTTCCAGTA 
GTACCTCCTT TCTTAAGTAG CATTGCTGGA GTCCCAACCA CAGCAGCAGC CACAGCACCA 
GTCCCTGCAA CAAGCAGCCC TCCTAATGAC ATTTCCACAT CAGTAATTCA GTCTGAGGTT 
ACAGTGCCCA CTGAAGAGGG GATTGCTGGA GTTGCCACCA GCACAGGTGT GGTAACTTCA 
GGTGGTCTCC CCATACCACC TGTGTCTGAA TCACCAGTAC TTTCCAGCGT AGTTTCAAGT 
ATCACAATAC CTGCAGTTGT CTCAATATCT ACTACATCCC CGTCACTTCA AGTCCCCACA 
TCCACATCTG AGATCGTTGT TTCTAGTACA GCACTGTATC CTTCAGTAAC AGTTTCAGCA 
ACTTCAGCCT CTGCAGGGGG CAGTACTGCT ACCCCAGGTC CTAAGCCTCC AGCTGTAGTA 
TCTCAGCAGG CAGCAGGCAG CACTACTGTG GGAGCCACAT TAACATCAGT TTCTACCACC 
ACTTCATTCC CAAGCACAGC TTCACAGCTG TGCATTCAGC TTAGCAGCAG TACTTCTACT 
CCTACTTTAG CTGAAACCGT GGTAGTTAGC GCACACTCAC TAGATAAGAC ATCTCATAGC 
AGTACAACTG GATTGGCTTT CTCCCTCTCT GCACCATCTT CCTCTTCCTC TCCTGGAGCA 
GGAGTGTCTA GTTATATTTC TCAGCCTGGT GGGCTGCATC CTTTGGTCAT TCCATCAGTG 
ATAGCTTCTA CTCCTATTCT TCCCCAAGCA GCAGGACCTA CTTCTACACC TTTATTACCC 
CAAGTACCTA GTATCCCACC CTTGGTACAG CCTGTTGCCA ATGTGCCTGC TGTACAGCAG 
ACACTAATTC ATAGTCAGCC TCAACCAGCT TTGCTTCCCA ACCAGCCCCA TACTCATTGT 
CCTGAAGTAG ATTCTGATAC ACAACCCAAA GCTCCTGGAA TTGATGACAT AAAGACTCTA 
GAAGAAAAGC TGCGGTCTCT GTTCAGTGAA CACAGCTCAT CTGGAGCTCA GCATGCCTCT 
GTCTCACTGG AGACCTCACT AGTCATAGAG AGCACTGTCA CACCAGGCAT CCCAACTACT 
GCTGTTGCAC CAAGCAAACT CCTGACTTCT ACCACAAGTA CTTGCTTACC ACCAACCAAT 
TTACCACTAG GAACAGTTGC TTTGCCAGTT ACACCAGTGG TCACACCTGG GCAAGTTTCT 
ACCCCAGTCA GCACTACTAC ATCAGGAGTG AAACCTGGAA CTGCTCCCTC CAAGCCACCT 
CTAACTAAGG CTCCGGTGCT GCCAGTGGGT ACTGAACTTC CAGCAGGTAC TCTACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCT CTAACCCAGT CCCAGCAACC TCTAGAGGAT 
CTTGATGCTC AATTGAGAAG AACACTTAGT CCAGAGATGA TCACAGTGAC TTCTGCGGTT 
GGTCCTGTGT CCATGGCGGC TCCAACAGCA ATCACAGAAG CAGGAACACA GCCTCAGAAG 
GGTGTTTCTC AAGTCAAAGA AGGCCCTGTC CTAGCAACTA GTTCAGGAGC TGGTGTTTTT 
AAGATGGGAC GATTTCAGGT TTCTGTTGCA GCAGACGGTG CCCAGAAAGA GGGTAAAAAT 
AAGTCAGAAG ATGCAAAGTC TGTTCATTTT GAATCCAGCA CCTCAGAGTC CTCAGTGCTA 
TCAAGTAGTA GTCCAGAGAG TACCTTGGTG AAACCAGAGC CGAATGGCAT AACCATCCCT 
GGTATCTCTT CAGATGTGCC AGAGAGTGCC CACAAAACTA CTGCCTCAGA GGCAAAGTCA 
GACACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTG 
GGTCGTTTCT CTGTATCAAA AACTGAGGAC AAGATCACTG ACACAAAGAA AGAAGGACCA 
GTGGCATCTC CTCCTTTTAT GGATTTGGAA CAAGCTGTTC TTCCTGCTGT GATACCAAAG 
AAAGAGAAGC CTGAACTGTC AGAGCCTTCA CATCTAAATG GGCCGTCTTC TGACCCGGAG 
GCCGCTTTTT TAAGTAGGGA TGTGGATGAT GGTTCCGGTA GTCCACACTC GCCCCATCAG 
CTGAGCTCAA AGAGCCTTCC TAGCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTTAAC 
TCCTCTTACA TGAGTAGCGA CAATGAGTCA GATATCGAAG ATGAAGACTT AAAGTTAGAG 
CTGCGACGAC TACGAGATAA ACATCTCAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTGTA TACCAAACTG GGCAAGGTGC CCCCTGCTGT TATTATTCCC 
CCAGCTGCTC CCCTTTCAGG GAGAAGACGA CGACCCACTA AAAGCAAAGG CAGCAAATCT 
AGTCGAAGCA GTTCCTTGGG GAATAAAAGC CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCAGCTT CAGTCTTGCA CCCCCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCAGAG 
TCCGGGCAGA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAT 
TCAGCCTTCA CCAGTGATGG TGCCATTTCA GTACCAAGCC TTTCTGCTCC AGGTCAAGGA 
ACCAGCAGCA CAAACACTGT TGGGGCAACA GTGAACAGCC AAGCCGCCCA AGCTCAGCCT 
CCTGCCATGA CGTCCAGCAG GAAGGGCACA TTCACAGATG ACTTGCACAA GTTGGTAGAC 
AATTGGGCCC GAGATGCCAT GAATCTCTCA GGCAGGAGAG GAAGCAAAGG GCACATGAAT 
TACGAGGGCC CTGGAATGGC AAGGAAGTTC TCTGCACCTG GGCAACTGTG CATCTCCATG 
ACCTCGAACC TGGGTGGCTC TGCCCCCATC TCTGCAGCAT CAGCTACCTC TCTAGGTCAC 
TTCACCAAGT CTATGTGCCC CCCACAGCAG TATGGCTTTC CAGCTACCCC ATTTGGCGCT 
CAATGGAGTG GGACGGGTGG CCCAGCACCA CAGCCACTTG GCCAGTTCCA ACCTGTGGGA 
ACTGCCTCCT TGCAGAATTT CAACATCAGC AATTTGCAGA AATCCATCAG CAACCCCCCA 
GGCTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011520997.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519299.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

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CloneID	OHu71613
Clone ID Related Accession (Same CDS sequence)	XM_011520997.2 , XM_011520997.3
Accession Version	XM_011520997.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC 
CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG 
GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA 
ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT 
GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG 
GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA 
AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC 
CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA 
CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT 
TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA 
CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG 
GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC 
ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT 
GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT 
GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA 
GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA 
ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC 
GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA 
GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA 
ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG 
GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC 
ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT 
GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA 
AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC 
GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT 
CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT 
CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG 
GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC 
ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG 
CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC 
CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA 
TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGTCA AGGAACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC 
AGCCAAGCCG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACA 
GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG 
AGAGGAAGCA AAGGGCACAT GAATTACGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA 
CCTGGGCAAC TGTGCATCTC CATGACCTCG AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC 
TTTCCAGCTA CCCCATTTGG CGCTCAATGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519299.1
CDS	1015..9402
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011520997.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520997.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC 
CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG 
GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA 
ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT 
GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG 
GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA 
AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC 
CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA 
CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT 
TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA 
CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG 
GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC 
ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT 
GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT 
GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA 
GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA 
ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC 
GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA 
GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA 
ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG 
GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC 
ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT 
GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA 
AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC 
GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT 
CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT 
CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG 
GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC 
ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG 
CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC 
CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA 
TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGTCA AGGAACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC 
AGCCAAGCCG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACA 
GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG 
AGAGGAAGCA AAGGGCACAT GAATTACGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA 
CCTGGGCAAC TGTGCATCTC CATGACCTCG AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC 
TTTCCAGCTA CCCCATTTGG CGCTCAATGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011520997.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519299.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

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CloneID	OHu71613
Clone ID Related Accession (Same CDS sequence)	XM_011520997.2 , XM_011520997.3
Accession Version	XM_011520997.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011520997.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC 
CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG 
GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA 
ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT 
GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG 
GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA 
AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC 
CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA 
CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT 
TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA 
CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG 
GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC 
ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT 
GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT 
GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA 
GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA 
ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC 
GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA 
GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA 
ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG 
GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC 
ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT 
GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA 
AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC 
GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT 
CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT 
CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG 
GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC 
ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG 
CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC 
CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA 
TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGTCA AGGAACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC 
AGCCAAGCCG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACA 
GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG 
AGAGGAAGCA AAGGGCACAT GAATTACGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA 
CCTGGGCAAC TGTGCATCTC CATGACCTCG AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC 
TTTCCAGCTA CCCCATTTGG CGCTCAATGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519299.1
CDS	974..9361
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011520997.3
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520997.3

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC 
CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG 
GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA 
ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT 
GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG 
GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA 
AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC 
CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA 
CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT 
TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA 
CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG 
GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC 
ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT 
GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT 
GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA 
GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA 
ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC 
GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA 
GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA 
ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG 
GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC 
ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT 
GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA 
AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC 
GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT 
CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT 
CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG 
GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC 
ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG 
CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC 
CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA 
TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGTCA AGGAACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC 
AGCCAAGCCG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACA 
GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG 
AGAGGAAGCA AAGGGCACAT GAATTACGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA 
CCTGGGCAAC TGTGCATCTC CATGACCTCG AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC 
TTTCCAGCTA CCCCATTTGG CGCTCAATGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011520998.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519300.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

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CloneID	OHu71614
Clone ID Related Accession (Same CDS sequence)	XM_011520998.2
Accession Version	XM_011520998.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA 
GTTGCGACAC AACCCTCGGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCAGTGGGA 
CAGCCGCTCC CTACTCCCTT GCTCCCTCAG TACCCTGTCT CTCAGATTCC CATATCAACT 
CCTCATGTGT CTACGGCTCA GACAGGTTTC TCATCCCTTC CCATCACAAT GGCAGCTGGC 
ATTACTCAGC CTCTGCTCAC GTTGGCTTCA TCTGCTACAA CAGCTGCGAT CCCGGGGGTA 
TCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGTTCACC CACAGCTCCT ACAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGTTCC ACTTTCCTCT GGAGATGTTC TGTACCAGGG CTTCCCACCT 
CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT 
GTTTGCATCC ATTCTACAGT CCTATCCCCT CCCATGCCGA CAGAAGTACT GGCTACACCT 
GGGTACTTTC CCACAGTGGT GCAGCCTTAT GTGGAATCAA ATCTTTTAGT TCCTATGGGT 
GGTGTAGGAG GACAGGTTCA AGTGTCCCAG CCAGGAGGGA GTTTAGCACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGT TTTGGAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTGCA 
GAGCCAGTTG CAGTAGCACA GACCCAAGCT ACCCAGCCGA CCACTTTGGC TTCCTCTGTA 
GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA CGTCCCATCT 
TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT 
CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA 
GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA 
TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT 
ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGATCAGGG ATTGGAGAGT 
CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA 
CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT 
CAAGTTGTTC ATTCTGCGGG AAGGCGGTTT ATAGTGAGTC CTGTGCCAGA AAGCCGATTA 
CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACAGCTCAG 
AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT 
GAACTTAGAC GTGCCCAAAT GACAGAAGGA CCCAACACAG CACCTCCAAA CTTTAGTCAT 
ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC 
ACAGCAGCAG CCACAGCACC AGTCCCTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA 
TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC 
AGCACAGGTG TGGTAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA 
CTTTCCAGCG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC 
CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGATCGTTG TTTCTAGTAC AGCACTGTAT 
CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT 
CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA 
TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTGCATTCAG 
CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA 
CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT 
TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTATATTT CTCAGCCTGG TGGGCTGCAT 
CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT 
ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA GCCTGTTGCC 
AATGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC 
AACCAGCCCC ATACTCATTG TCCTGAAGTA GATTCTGATA CACAACCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TGTTCAGTGA ACACAGCTCA 
TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC 
ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TACCACAAGT 
ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG 
GTCACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGAGT GAAACCTGGA 
ACTGCTCCCT CCAAGCCACC TCTAACTAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT 
CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG 
TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATG 
ATCACAGTGA CTTCTGCGGT TGGTCCTGTG TCCATGGCGG CTCCAACAGC AATCACAGAA 
GCAGGAACAC AGCCTCAGAA GGGTGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT 
AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519300.1
CDS	969..9353
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQAQGQSQGQPSSSSLTGVSSSQPIQHPQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011520998.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520998.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA 
GTTGCGACAC AACCCTCGGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCAGTGGGA 
CAGCCGCTCC CTACTCCCTT GCTCCCTCAG TACCCTGTCT CTCAGATTCC CATATCAACT 
CCTCATGTGT CTACGGCTCA GACAGGTTTC TCATCCCTTC CCATCACAAT GGCAGCTGGC 
ATTACTCAGC CTCTGCTCAC GTTGGCTTCA TCTGCTACAA CAGCTGCGAT CCCGGGGGTA 
TCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGTTCACC CACAGCTCCT ACAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGTTCC ACTTTCCTCT GGAGATGTTC TGTACCAGGG CTTCCCACCT 
CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT 
GTTTGCATCC ATTCTACAGT CCTATCCCCT CCCATGCCGA CAGAAGTACT GGCTACACCT 
GGGTACTTTC CCACAGTGGT GCAGCCTTAT GTGGAATCAA ATCTTTTAGT TCCTATGGGT 
GGTGTAGGAG GACAGGTTCA AGTGTCCCAG CCAGGAGGGA GTTTAGCACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGT TTTGGAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTGCA 
GAGCCAGTTG CAGTAGCACA GACCCAAGCT ACCCAGCCGA CCACTTTGGC TTCCTCTGTA 
GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA CGTCCCATCT 
TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT 
CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA 
GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA 
TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT 
ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGATCAGGG ATTGGAGAGT 
CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA 
CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT 
CAAGTTGTTC ATTCTGCGGG AAGGCGGTTT ATAGTGAGTC CTGTGCCAGA AAGCCGATTA 
CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACAGCTCAG 
AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT 
GAACTTAGAC GTGCCCAAAT GACAGAAGGA CCCAACACAG CACCTCCAAA CTTTAGTCAT 
ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC 
ACAGCAGCAG CCACAGCACC AGTCCCTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA 
TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC 
AGCACAGGTG TGGTAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA 
CTTTCCAGCG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC 
CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGATCGTTG TTTCTAGTAC AGCACTGTAT 
CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT 
CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA 
TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTGCATTCAG 
CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA 
CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT 
TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTATATTT CTCAGCCTGG TGGGCTGCAT 
CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT 
ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA GCCTGTTGCC 
AATGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC 
AACCAGCCCC ATACTCATTG TCCTGAAGTA GATTCTGATA CACAACCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TGTTCAGTGA ACACAGCTCA 
TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC 
ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TACCACAAGT 
ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG 
GTCACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGAGT GAAACCTGGA 
ACTGCTCCCT CCAAGCCACC TCTAACTAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT 
CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG 
TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATG 
ATCACAGTGA CTTCTGCGGT TGGTCCTGTG TCCATGGCGG CTCCAACAGC AATCACAGAA 
GCAGGAACAC AGCCTCAGAA GGGTGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT 
AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011520999.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519301.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

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CloneID	OHu71615
Clone ID Related Accession (Same CDS sequence)	XM_011520999.2
Accession Version	XM_011520999.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520999.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC 
CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG 
GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA 
ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT 
GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG 
GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA 
AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC 
CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA 
CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT 
TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA 
CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG 
GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC 
ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT 
GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT 
GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA 
GAAGCAGGAA CACAGCCTCA GAAGGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT 
AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519301.1
CDS	969..9353
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011520999.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520999.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC 
CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG 
GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA 
ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT 
GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG 
GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA 
AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC 
CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA 
CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT 
TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA 
CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG 
GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC 
ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT 
GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT 
GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA 
GAAGCAGGAA CACAGCCTCA GAAGGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT 
AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011521000.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519302.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

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CloneID	OHu71616
Clone ID Related Accession (Same CDS sequence)	XM_011521000.2
Accession Version	XM_011521000.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC 
CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG 
GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA 
ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT 
GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG 
GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA 
AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC 
CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA 
CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT 
TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA 
CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG 
GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC 
ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT 
GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT 
GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA 
GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA 
ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC 
GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA 
GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA 
ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG 
GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC 
ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT 
GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA 
AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC 
GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT 
CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT 
CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG 
GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC 
ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG 
CTTTCAGGAA CCAGCAGCAC AAACACTGTT GGGGCAACAG TGAACAGCCA AGCCGCCCAA 
GCTCAGCCTC CTGCCATGAC GTCCAGCAGG AAGGGCACAT TCACAGATGA CTTGCACAAG 
TTGGTAGACA ATTGGGCCCG AGATGCCATG AATCTCTCAG GCAGGAGAGG AAGCAAAGGG 
CACATGAATT ACGAGGGCCC TGGAATGGCA AGGAAGTTCT CTGCACCTGG GCAACTGTGC 
ATCTCCATGA CCTCGAACCT GGGTGGCTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT 
CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ATGGCTTTCC AGCTACCCCA 
TTTGGCGCTC AATGGAGTGG GACGGGTGGC CCAGCACCAC AGCCACTTGG CCAGTTCCAA 
CCTGTGGGAA CTGCCTCCTT GCAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC 
AACCCCCCAG GCTCCAACCT GCGGACCACT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519302.1
CDS	969..9161
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011521000.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521000.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC 
CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG 
GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA 
ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT 
GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG 
GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA 
AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC 
CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA 
CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT 
TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA 
CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG 
GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC 
ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT 
GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT 
GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT 
GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA 
GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA 
ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC 
GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA 
GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA 
ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG 
GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC 
ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT 
GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA 
AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC 
GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT 
CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT 
CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG 
GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC 
ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG 
CTTTCAGGAA CCAGCAGCAC AAACACTGTT GGGGCAACAG TGAACAGCCA AGCCGCCCAA 
GCTCAGCCTC CTGCCATGAC GTCCAGCAGG AAGGGCACAT TCACAGATGA CTTGCACAAG 
TTGGTAGACA ATTGGGCCCG AGATGCCATG AATCTCTCAG GCAGGAGAGG AAGCAAAGGG 
CACATGAATT ACGAGGGCCC TGGAATGGCA AGGAAGTTCT CTGCACCTGG GCAACTGTGC 
ATCTCCATGA CCTCGAACCT GGGTGGCTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT 
CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ATGGCTTTCC AGCTACCCCA 
TTTGGCGCTC AATGGAGTGG GACGGGTGGC CCAGCACCAC AGCCACTTGG CCAGTTCCAA 
CCTGTGGGAA CTGCCTCCTT GCAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC 
AACCCCCCAG GCTCCAACCT GCGGACCACT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_011521001.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519303.1 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

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CloneID	OHu71617
Clone ID Related Accession (Same CDS sequence)	XM_011521001.2
Accession Version	XM_011521001.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase WNK1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC 
CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG 
GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA 
ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT 
GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG 
GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA 
AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC 
CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG 
CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC 
GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT 
ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG 
GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC 
CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC 
TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG 
CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT 
GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC 
ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA 
ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519303.1
CDS	969..9077
Translation	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQSTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_011521001.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521001.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA 
GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG 
GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC 
GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC 
CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG 
GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA 
ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT 
GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG 
GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA 
AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC 
CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG 
CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC 
GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT 
ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG 
GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC 
CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC 
TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG 
CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT 
GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC 
ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA 
ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	WNK1
Entrez Gene ID	65125
Full Name	WNK lysine deficient protein kinase 1
Synonyms	HSAN2,HSN2,KDP,PPP1R167,PRKWNK1,PSK,p65
General protein information	Preferred Names WNK lysine deficient protein kinase 1 Names serine/threonine-protein kinase WNK1 WNK lysine deficient protein kinase 1 isoform erythrocyte 65 kDa protein prostate-derived sterile 20-like kinase protein kinase with no lysine 1 protein phosphatase 1, regulatory subunit 167 serine/threonine-protein kinase WNK1 1 serine/threonine-protein kinase WNK1 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.33
Summary	This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010].
Disorder MIM:	605232
Disorder Html:	Pseudohypoaldosteronism, type IIC, 614492 (3); Neuropathy, hereditary sensory and autonomic, type II, 201300 (3)

Canis lupus familiaris (dog)	WNK1	XP_003433530.1
Mus musculus (house mouse)	Wnk1	NP_001186013.1
Bos taurus (cattle)	WNK1	XP_005207339.1
Homo sapiens (human)	WNK1	NP_001171914.1
Rattus norvegicus (Norway rat)	Wnk1	NP_001002823.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

WNK1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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