MGG_11636 cDNA ORF clone, Pyricularia oryzae 70-15

The following MGG_11636 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MGG_11636 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMe09297 XM_003718864.1
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Pyricularia oryzae 70-15 serine/threonine protein kinase (MGG_11636), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMe09297
    Clone ID Related Accession (Same CDS sequence) XM_003718864.1
    Accession Version XM_003718864.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-28
    Organism Pyricularia oryzae 70-15
    Product serine/threonine protein kinase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_017852).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACCACCT ACGTATATCC CAGCGCCTTC AGTCCGTTGA CCTCTCAGCT TGGTGTGAGC 
    GTCGGAGCCG GGGCCGCAGG AGCCATCACA TCAGCCAACG ACGAAGCCAC GACTTCGTCA
    AAGTCTGCCA AGGACGCCAG CTTCGCGACC TTCATCTGCC ACACTGCCTA CCTCGAGGGG
    CAGCTACGAA AGGGCGACCA GTCTGCATTC TTTGCAGCTG GTGGGAAGCA AATTGTTAAT
    CGTAAGAGGA TTGGAGAGGG CGCTTCGTTC CTCGTCGACC GGGCCGAATT GCAAAGCAAC
    ACCGAGACAG ACGGCAGCCA AGGCCCCAAG TACGTCGCGG TAAAGACGGC CAAAGAACCG
    ACTGCCGACG CCGAAAGCAG TGGAAGATGG CGCGATATTT TGTTCGAGAT ACGCGCCCTG
    CTGCACGAGC CCCTCCGCTA CCACCCAAAC ATTGTTCACC TTCTCGACAT TCGCTGGGAC
    GGCAGCGAGA CAGGCTCTCC CTTCCCGACG CTGGTGGTTG AATATGCCGA GTCCGGGACC
    CTGCTCAGTC TGCAGAGATC ACATTCCCAG CATCCCCTCC CCTTCTCCAT CAAACAGAAG
    CTATGCTACG ATGTCGGCAG AGGGCTGTCG ATCCTTCACG CGTGCGGTAT CGTCCACGGT
    GACCTGAAGC ACGAAAACGT GCTCATATTC GCGAACCGCA ATCAAAACCC TCCCGGCCAG
    CCTTACACGG CCAAGCTTGC CGATTTCGGT GGCACCGTGA TGGACATGGT GGACGACGAC
    GGGCGCGAGA TGCAGGGCCA CCGGCTGCGC GTCAGCACGT TTCCCTACGA GGCGCCCGAG
    GTCGGCAGGC AGCTGTCCAC CGACGGGGCC AAGAAGACGG ACGCATACTC GTACGGCATG
    CTCATCTGGC GCTGCATGCT CGACTGCGCC GACATGTTCC TTCTGAGCGG CCTGCAGCTG
    CCCAAGGGCC GACCCGAGGA GGCAGAGCTG GAGAGGATGA ACGAGTACAA GCGGTCGGAT
    CAGGTGCTGG AGTGGGCCAT GTCTACGATT TCGGAACATT TTATCAGAAA GACAATACCG
    GCGGCGAGCT TGCAGTTGGT CATGGCCGCC CTCATGTTCA CCCTCCGCGG GGACCCGGGG
    CAGCGGGCCT TGGATCGGGC GCAGGCGCGC ATGAGGGGTA TGACGGCGAA TTTGGGCTAT
    TCTTATACCC TGGTCAAGGA CGAGGCCAAC AGGAGGACGA CAGAGAGTGA GCGTTACAGG
    ACCCCGGGCA GCCATGGATT GACGGTGGAT ACGGTGGGTT ATGGGCTTGG AAGATTGGGG
    GACGAGTACG ACGCGCAGAA CAACTTGCCC GGTTACCGGC CCGACCTACC TCATCCTGAA
    AGAGGTGGCT TCCACTTTGA TCCGCTGACT CTACGCGGTC TGCTCGACTG GGATCAACAA
    GTGGCCATGC TGGACGAGTT CAAGGCCGCC GCCGAGGGGG GAGCCCAAGC AACAGTAACA
    AGCCTACAGC CCTGGACGGC CGCATTCTTC CTGTACCAGT GTTACATTGT CGGATTCGGT
    ACCCAAATAA ACGCACAAGA GGCCTGCAGG TGGCTGTCCA AGGCAGCCGA CCCTCCCGAA
    GAGATTGCCG GCGTGGACTA CCTGGCGAGA GCATGGCTCA CGCGCGTATA CTCGGCGCTC
    GGCGTCGACA TCCCCCTCAG CCCCGCACAG CAAAAGGAAA ACCTCTGGTG GAGCGTCATC
    CGAGGTCACC GGCTGTGCTC TGTCGACCTG CGCGCCCTTG CTATGCAATA CCCGCAAGAA
    GCCGCTCGAG AAGACTGGTT GGGATCTTTG GAAGAGGCCG AGTGGAACTT CAAGACGAGA
    ACCGGTGCGA CTGGCATGCC ATTTTTCATC CGACGAAAGC TGTCACGAGC TTGGTCCGTT
    GACGGAGACA TGGCGGCGCT CGACAACGAG ATCAGGATGG ACCTAGGCAG CCAGTACGAC
    TCTTGCGTCA GACAGTCAGC AGGCGAGAAC GGCCAAGAGG CCGGGGACGA CCAGGAGAAG
    CGCTTTGACA AGATCTACGT CAACCACAAA GGCCACGGTC TTCTTCACCT CGCGGCAGCG
    TATGGATCCG AGGCTGCCCT CCGCCACATG CTCCACAAGT ACGACTGCAG CATTGATCTG
    CGTAACCAGT CCCACAGCGA GACGCCGCTC CTCTGTGCCG TCAAGGCCGG AAACCCACAG
    TGCGTCGAGC TTCTCCTCGA CCGTGGCGCC CTGCCAGATG GCCACAAGCC CGGCAGCGAG
    GGCTTGGTGG AGGAAACCCC GCTGCACTGG CTGTCGAGCT TTGGTGAAAC CAGAGACGAG
    GAGGAATACA TGCACCGCTT GATCGACAGG CTGCAAGCGG CCGGGGCGGA CATTGAGAAG
    GTCACGGGCG GGGGCAGACC CGAGGTCAGG GGCATCATCG CGGACTGGGA GAATAGCCTC
    GGCATCCCGC TCACGCCTCT TGGACGTGCG GTCCTGCATC AAAGCCATGC GGCTGTTCGG
    AGGCTCTTGG CACATGGCGC CGACCCTCTC GGCAGGAGCA GCAAGACGGC GGCCAGGTCT
    CCACTGGAGC TGGCTGCCGT ATTGACACTG CCTGACATGC TGGAGCTTCT TCTCAAGGCG
    GTCGACGAGC GCGACTTGGA CGACGATGTT GACAAGCCCA TTGTCTGGGA CGAGAACGAA
    ATGCTCGAGG CGGCGCACAG CGGTGCCGTC ACGGGCTATG ACCCCCTGAC GCTGCAGAGC
    AGACTCGTGC GCTGCGGGTC AAACTACAAG GTTTGGCTTC GACGGACGCT ACAGATGCTG
    CGAGATCGAA GGCGCCATCA GGAAGGAGCC GCAGGGAAGG CTCTTGTTGC AGACTCCAGC
    GGAGGTGCGA GCCTCTGCCG TGAAATCAAG CTTGGAAACT CAGACATTGT CGAAGCACTC
    CTCAACATCG GCCATCCGGT AGAAGGGACG CCGGGTTCGC GGCCGATAGA GGCTGCCATC
    CTGTCGAATA ACGGCACCGC GTATGATATT CTCACCACGA ACCTTTCCCC ACTGAGCTTC
    CCGGAGAACG AAAACAAGAC TCTACTGCAG CTGTTTGCTT CCAGGCCAAG AACAGCACCA
    CGCGACACCG CCATCGCCGA GCATCTCATA GCCAGCGGCG TTTCCGTTGA GCCTCATCCC
    CAAAGCAGCC TTCCTGGAGC CTTAAGCCTT GCCATCTATA GGCGGTACTT TGACCTCGCA
    GACCTGCTGA TTGCACACGG CGCAGGATCA GAGCTCAACT CTTACTACAC ACCACCAAGC
    ACAACCGACA CGGACCAAGT CTCTCTGCTG GGGGCGCTCC TCAAACAACA CAGCTTTGCA
    ACGCTAGATT CCATCTCGTA CCTGGCGCGC ATCCACAGCG CTGGCAAAGT CAGCATCAGC
    CCGCTGGTCA ACCGGACGTG CAACCTCACC GCCGTCCACA TGCTATGCCT GCAGCCGGCA
    GAGGAATGGA ACAGCCACTC GCAGATCTCG GACTGCATAG TCCAGAAGAT CATCGAAATG
    TTTCCAGACA AGGAAAGCCT GGGAGAGCAC GCGGCGCACC CCAAACTCGG CACCCCTCTC
    AACGCCGCCA TCGTCGCCGC CAACACGCCC ATGGTCACCG CGCTCCTGGA TTCGGACCAC
    GTCGCCGCCG TCGACGCGCC CGAGCAGCCC AGCCAGCTCG ACTGCGACGG CGCCTCGCCC
    CTCAGCCCCC GCGTCCTCGC CACCTTGCTC GCCCAGCGCT CCATGGCCGA CTTTGAAGCC
    GCCCTGGCCG CCTCGACGGC CCCGCCAGCG CACGAGCTCG ACAAGCTGAA GCGCCGGTTC
    GACATTGCAG GCATGCTCGC CGTGCCGCCG GGCCAGGCCC CGCCCGACGC GCCCGCCGGC
    CTCGTCTTCC GGCACCCGAT CGAGTACGAC GCCAAGCTGC GGCGCTACGA GCTGGAGCTG
    GCGTCTGTCG GGGCAGCGAG GCCCGCCGAT CTCCACGTGC CGATTGTGGG GATGCGGTCG
    CTGGGCCTGG ACGAGCAGCA GCAGCAGCAG GGTGGGAGTT TGGGCGGTGA AGGTGGCAGC
    GGCAGCACGA CTAGAGATCC GAATCTGCCC GTTGACCTGT CCGTGCTGAC GGAGGAGCAG
    CCGACCGGGT GGCACGAGGG CGTCGAGATG ACGCAGCTCA TGGCCCTCAG GGTCTTTCTC
    AAGACCTTTC GCGGCTCCGA GGCTGGAAGG CTGGGGCTCG ATGGGACGAA TGCATTGATG
    AACCAGACTT ATAATAGGAA CAGGGCTGGC GCTGCAGATG GTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003718912.1
    CDS221..4528
    Translation

    Target ORF information:

    RefSeq Version XM_003718864.1
    Organism Pyricularia oryzae 70-15
    Definition Pyricularia oryzae 70-15 serine/threonine protein kinase (MGG_11636), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003718864.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACCACCT ACGTATATCC CAGCGCCTTC AGTCCGTTGA CCTCTCAGCT TGGTGTGAGC 
    GTCGGAGCCG GGGCCGCAGG AGCCATCACA TCAGCCAACG ACGAAGCCAC GACTTCGTCA
    AAGTCTGCCA AGGACGCCAG CTTCGCGACC TTCATCTGCC ACACTGCCTA CCTCGAGGGG
    CAGCTACGAA AGGGCGACCA GTCTGCATTC TTTGCAGCTG GTGGGAAGCA AATTGTTAAT
    CGTAAGAGGA TTGGAGAGGG CGCTTCGTTC CTCGTCGACC GGGCCGAATT GCAAAGCAAC
    ACCGAGACAG ACGGCAGCCA AGGCCCCAAG TACGTCGCGG TAAAGACGGC CAAAGAACCG
    ACTGCCGACG CCGAAAGCAG TGGAAGATGG CGCGATATTT TGTTCGAGAT ACGCGCCCTG
    CTGCACGAGC CCCTCCGCTA CCACCCAAAC ATTGTTCACC TTCTCGACAT TCGCTGGGAC
    GGCAGCGAGA CAGGCTCTCC CTTCCCGACG CTGGTGGTTG AATATGCCGA GTCCGGGACC
    CTGCTCAGTC TGCAGAGATC ACATTCCCAG CATCCCCTCC CCTTCTCCAT CAAACAGAAG
    CTATGCTACG ATGTCGGCAG AGGGCTGTCG ATCCTTCACG CGTGCGGTAT CGTCCACGGT
    GACCTGAAGC ACGAAAACGT GCTCATATTC GCGAACCGCA ATCAAAACCC TCCCGGCCAG
    CCTTACACGG CCAAGCTTGC CGATTTCGGT GGCACCGTGA TGGACATGGT GGACGACGAC
    GGGCGCGAGA TGCAGGGCCA CCGGCTGCGC GTCAGCACGT TTCCCTACGA GGCGCCCGAG
    GTCGGCAGGC AGCTGTCCAC CGACGGGGCC AAGAAGACGG ACGCATACTC GTACGGCATG
    CTCATCTGGC GCTGCATGCT CGACTGCGCC GACATGTTCC TTCTGAGCGG CCTGCAGCTG
    CCCAAGGGCC GACCCGAGGA GGCAGAGCTG GAGAGGATGA ACGAGTACAA GCGGTCGGAT
    CAGGTGCTGG AGTGGGCCAT GTCTACGATT TCGGAACATT TTATCAGAAA GACAATACCG
    GCGGCGAGCT TGCAGTTGGT CATGGCCGCC CTCATGTTCA CCCTCCGCGG GGACCCGGGG
    CAGCGGGCCT TGGATCGGGC GCAGGCGCGC ATGAGGGGTA TGACGGCGAA TTTGGGCTAT
    TCTTATACCC TGGTCAAGGA CGAGGCCAAC AGGAGGACGA CAGAGAGTGA GCGTTACAGG
    ACCCCGGGCA GCCATGGATT GACGGTGGAT ACGGTGGGTT ATGGGCTTGG AAGATTGGGG
    GACGAGTACG ACGCGCAGAA CAACTTGCCC GGTTACCGGC CCGACCTACC TCATCCTGAA
    AGAGGTGGCT TCCACTTTGA TCCGCTGACT CTACGCGGTC TGCTCGACTG GGATCAACAA
    GTGGCCATGC TGGACGAGTT CAAGGCCGCC GCCGAGGGGG GAGCCCAAGC AACAGTAACA
    AGCCTACAGC CCTGGACGGC CGCATTCTTC CTGTACCAGT GTTACATTGT CGGATTCGGT
    ACCCAAATAA ACGCACAAGA GGCCTGCAGG TGGCTGTCCA AGGCAGCCGA CCCTCCCGAA
    GAGATTGCCG GCGTGGACTA CCTGGCGAGA GCATGGCTCA CGCGCGTATA CTCGGCGCTC
    GGCGTCGACA TCCCCCTCAG CCCCGCACAG CAAAAGGAAA ACCTCTGGTG GAGCGTCATC
    CGAGGTCACC GGCTGTGCTC TGTCGACCTG CGCGCCCTTG CTATGCAATA CCCGCAAGAA
    GCCGCTCGAG AAGACTGGTT GGGATCTTTG GAAGAGGCCG AGTGGAACTT CAAGACGAGA
    ACCGGTGCGA CTGGCATGCC ATTTTTCATC CGACGAAAGC TGTCACGAGC TTGGTCCGTT
    GACGGAGACA TGGCGGCGCT CGACAACGAG ATCAGGATGG ACCTAGGCAG CCAGTACGAC
    TCTTGCGTCA GACAGTCAGC AGGCGAGAAC GGCCAAGAGG CCGGGGACGA CCAGGAGAAG
    CGCTTTGACA AGATCTACGT CAACCACAAA GGCCACGGTC TTCTTCACCT CGCGGCAGCG
    TATGGATCCG AGGCTGCCCT CCGCCACATG CTCCACAAGT ACGACTGCAG CATTGATCTG
    CGTAACCAGT CCCACAGCGA GACGCCGCTC CTCTGTGCCG TCAAGGCCGG AAACCCACAG
    TGCGTCGAGC TTCTCCTCGA CCGTGGCGCC CTGCCAGATG GCCACAAGCC CGGCAGCGAG
    GGCTTGGTGG AGGAAACCCC GCTGCACTGG CTGTCGAGCT TTGGTGAAAC CAGAGACGAG
    GAGGAATACA TGCACCGCTT GATCGACAGG CTGCAAGCGG CCGGGGCGGA CATTGAGAAG
    GTCACGGGCG GGGGCAGACC CGAGGTCAGG GGCATCATCG CGGACTGGGA GAATAGCCTC
    GGCATCCCGC TCACGCCTCT TGGACGTGCG GTCCTGCATC AAAGCCATGC GGCTGTTCGG
    AGGCTCTTGG CACATGGCGC CGACCCTCTC GGCAGGAGCA GCAAGACGGC GGCCAGGTCT
    CCACTGGAGC TGGCTGCCGT ATTGACACTG CCTGACATGC TGGAGCTTCT TCTCAAGGCG
    GTCGACGAGC GCGACTTGGA CGACGATGTT GACAAGCCCA TTGTCTGGGA CGAGAACGAA
    ATGCTCGAGG CGGCGCACAG CGGTGCCGTC ACGGGCTATG ACCCCCTGAC GCTGCAGAGC
    AGACTCGTGC GCTGCGGGTC AAACTACAAG GTTTGGCTTC GACGGACGCT ACAGATGCTG
    CGAGATCGAA GGCGCCATCA GGAAGGAGCC GCAGGGAAGG CTCTTGTTGC AGACTCCAGC
    GGAGGTGCGA GCCTCTGCCG TGAAATCAAG CTTGGAAACT CAGACATTGT CGAAGCACTC
    CTCAACATCG GCCATCCGGT AGAAGGGACG CCGGGTTCGC GGCCGATAGA GGCTGCCATC
    CTGTCGAATA ACGGCACCGC GTATGATATT CTCACCACGA ACCTTTCCCC ACTGAGCTTC
    CCGGAGAACG AAAACAAGAC TCTACTGCAG CTGTTTGCTT CCAGGCCAAG AACAGCACCA
    CGCGACACCG CCATCGCCGA GCATCTCATA GCCAGCGGCG TTTCCGTTGA GCCTCATCCC
    CAAAGCAGCC TTCCTGGAGC CTTAAGCCTT GCCATCTATA GGCGGTACTT TGACCTCGCA
    GACCTGCTGA TTGCACACGG CGCAGGATCA GAGCTCAACT CTTACTACAC ACCACCAAGC
    ACAACCGACA CGGACCAAGT CTCTCTGCTG GGGGCGCTCC TCAAACAACA CAGCTTTGCA
    ACGCTAGATT CCATCTCGTA CCTGGCGCGC ATCCACAGCG CTGGCAAAGT CAGCATCAGC
    CCGCTGGTCA ACCGGACGTG CAACCTCACC GCCGTCCACA TGCTATGCCT GCAGCCGGCA
    GAGGAATGGA ACAGCCACTC GCAGATCTCG GACTGCATAG TCCAGAAGAT CATCGAAATG
    TTTCCAGACA AGGAAAGCCT GGGAGAGCAC GCGGCGCACC CCAAACTCGG CACCCCTCTC
    AACGCCGCCA TCGTCGCCGC CAACACGCCC ATGGTCACCG CGCTCCTGGA TTCGGACCAC
    GTCGCCGCCG TCGACGCGCC CGAGCAGCCC AGCCAGCTCG ACTGCGACGG CGCCTCGCCC
    CTCAGCCCCC GCGTCCTCGC CACCTTGCTC GCCCAGCGCT CCATGGCCGA CTTTGAAGCC
    GCCCTGGCCG CCTCGACGGC CCCGCCAGCG CACGAGCTCG ACAAGCTGAA GCGCCGGTTC
    GACATTGCAG GCATGCTCGC CGTGCCGCCG GGCCAGGCCC CGCCCGACGC GCCCGCCGGC
    CTCGTCTTCC GGCACCCGAT CGAGTACGAC GCCAAGCTGC GGCGCTACGA GCTGGAGCTG
    GCGTCTGTCG GGGCAGCGAG GCCCGCCGAT CTCCACGTGC CGATTGTGGG GATGCGGTCG
    CTGGGCCTGG ACGAGCAGCA GCAGCAGCAG GGTGGGAGTT TGGGCGGTGA AGGTGGCAGC
    GGCAGCACGA CTAGAGATCC GAATCTGCCC GTTGACCTGT CCGTGCTGAC GGAGGAGCAG
    CCGACCGGGT GGCACGAGGG CGTCGAGATG ACGCAGCTCA TGGCCCTCAG GGTCTTTCTC
    AAGACCTTTC GCGGCTCCGA GGCTGGAAGG CTGGGGCTCG ATGGGACGAA TGCATTGATG
    AACCAGACTT ATAATAGGAA CAGGGCTGGC GCTGCAGATG GTAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the rice blast fungus Magnaporthe grisea.
    Nature434(7036)980-6(2005 Apr)
    Dean RA,Talbot NJ,Ebbole DJ,Farman ML,Mitchell TK,Orbach MJ,Thon M,Kulkarni R,Xu JR,Pan H,Read ND,Lee YH,Carbone I,Brown D,Oh YY,Donofrio N,Jeong JS,Soanes DM,Djonovic S,Kolomiets E,Rehmeyer C,Li W,Harding M,Kim S,Lebrun MH,Bohnert H,Coughlan S,Butler J,Calvo S,Ma LJ,Nicol R,Purcell S,Nusbaum C,Galagan JE,Birren BW