MGG_09995 cDNA ORF clone, Pyricularia oryzae 70-15

The following MGG_09995 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MGG_09995 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMe05362 XM_003717465.1
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Pyricularia oryzae 70-15 glucan 1,3-beta-glucosidase (MGG_09995), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMe05362
    Clone ID Related Accession (Same CDS sequence) XM_003717465.1
    Accession Version XM_003717465.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-28
    Organism Pyricularia oryzae 70-15
    Product glucan 1,3-beta-glucosidase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_017851).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGTACG GAACCCTCAT GTTCTGGGCA CTATGCCTAG TGCCCCACCA CGCCTTTGCG 
    GTAAATAATA TCGACAGTGA ACAGTATGCG GTTGGGTCGG AGGCTTGGAA ACGAGCAGGA
    AACCACTTGA ACGAGCACAT CCCGGACCTG GCCCTGCATA AACGCCAGAT ACGAACCGCC
    GATGCGATCC CCCTCCGCGT CAGGAGCGCA ACGCAATCGG ATATCCATGG CGCTCGGGAT
    ATCGTCCAGG CCGCCATGGC CGAAGCCGGT GTGCGCAACC AGAAGCGCAT GCTGAACCCG
    GCTCGCAACA CATACTATGC CGACCAAATA TTCGACTCGA CGCTCCAGAA GCGCGATTAC
    ATGGTTCCAC AGCTGCCCAA GGTTTCCGAC GAAGCTGCTG CTGCCGCTGC GCTCTTGGCC
    GAGGTAGACT CGCTTCAGTC TCGCCGATCG CTAGCCAAGA GGGACGGCAA CGACAAGAGC
    AAGTTCTGGA TGGAGAACAT TGCGCGCAAG GGTTCCATGC CTTGGGGCGA TGATCCGGAC
    TACCAGGTCT TTCGCAACGT CAAGGACTTT GGCGCCAAGG GCGATGGCAT TCACGATGAC
    ACCTTTGCGA TCCGACGTGC CATCCTCGCC GCATCTGGCA AGAAGCCTGA AGTAAACGCC
    CGCTGCACGG GAGATTGCGC TGGCTCCACG ACGCAGAACG CCATTGTGTA CTTTCCTTCA
    GGCACTTACC TTGTCAGCGG CACGATTCCA GCTTTGCCAG CGACGCAGCT CATCGGCGAT
    GCCACCAACC CGCCTACCCT GAAGGCTGCC CCGTCGTTTC TTGGGCTGGG AGTCATCTCG
    ACAAAGGAGT TCCGCGACGA CACCAACGTA ACCAAGATCA AGGACAAGCG AGACGACTCG
    AACGAGGTTT CCACGGCGGC TGGCGATAGC TTTTATCGCC AAATCAGAAA CTTGATTGTC
    GACATCACCT CGGCACCCCA GGACGGCTAC ATCAGTGGCA TCCACTACCA CACATCGCAA
    GCGGCCTCGC TCTCATTTGT GGAGATTCAC GCAACCAGCA TCAAAAGCAA TCCCAACACG
    ACACAAATAG GAATCTTCTC CGAGAATGGC AGCGGAGGTC AAATCAGCGA TCTGACCGTC
    GTGGGAGGAA AAGTCGGCTT CTATGGAGGA AACTCGCAGT TCACCGCTCA GCGCATCAAG
    TTCCGCGAGT GTGGCACCGC AGTTCGGCTT CTCTGGGACT GGAGCTGGGT ATGGAAGAGC
    GTGGAGGTTG CCAACTGCAA CACGGGATTC CAGCTCCACG GCGGCGACAC CTTGAACAGC
    GAGAGTGAAC CCGGTGGCTA TCTGGGATCC ATCCACGTCA TGGACTCGAC CTTTGACAAT
    GTCGGCACCG CTGTCCGCAA TGCGCCCGCG TCGCACGAGC CTGGCGAGGA GACCACTAGC
    ATCACCCTCG ACAATGTTGC CTTTACCGGG CGGGTGGAAC ATCGCGTCTG GGACGGAGAG
    AAAGAGTACG TGCAGGGAAC GCCCAGCAAG CTCGACACGT GGACTTTGGG CGCTCTGTAT
    CTGGATCCTT CCAAGCGTGA CCTGTCTCTG GGTTTTGAGC ACGAGACTCG TCGTGAAGAC
    GGCCTCACTG GAAACAAGAC GAACCCCTCG TTGCCCAAAA AGGCCTACTT TGAGCGTTCC
    AAGCCCCAGT ACACCGATGT CCCCGCCCAA AATTGGGTGC ATACCAAGGA CTCGTGCAAG
    GGAGACGGCA AGGCAGACGA CACAGCGTGT CTGCAGAAGG TCCTGGACAA GAAAGGTTAC
    ATTTTTGTCG ATGCGGGCGT CTATCTGCTA TCCAATACCG TCACCGTCCA CCCGGGCACT
    TTCATCGTCG GCGAGGCATG GTCACAGCTC GTCGCTCAGG GCAACAAGTT CTCCGACGCC
    GGCCAGCCAC GACCCATGAT CCGTGTCGGA AGCAAAGGCG ACAAGGGCTC GGTGGAGATG
    CAGGACCTGC TCTTTACGAC CAAGGGCGCC ACATCCGGCG CCATCCTGGT CGAGTGGAAC
    ATCAAGGCCG AAGGGGAGGG CAAAGCCGGC ATGTGGGACT GCCACGCCCG CGTCGGCGGC
    GCCAGAGGCA CCGAGCTGAC GCCCAAGGAG TGCCCCGCGT CGCCCTCTGG CGGCAGCAGG
    CGACAGTGCC ACGCCGCCGC CCTTCTGATG CACATCACTC CCGGCGCGTC GGCCTACCTC
    GAGAACGTCT GGCTGTGGGC CGCGGACCAC ATGGTTGACG GCGCCTTGCC CGGCCGAGCC
    GAGCGCACCT CGGTGTACGC GGCTCGTGGC ATGCTCATCG AGAGCAGCGA TCCGACGTGG
    CTCTACGGCA CGTCAGCGGA GCACGCTGCC ATGTACCAGT ACAACTTTTA CAGGAGCAAA
    AAGGTCTTTG CGGCCATGAT CAGAACCGAG GCGCCGTTTT TCCAGGATGA GAGGCCGATG
    TTGGTCCAGA GGGGTATCAG TTCGCTGTTT TCGGGGGATC CGATCTGCAC CGCCGCGGAT
    CTTTGCGGAA AGGCTCTGGG CGCCATCGTC AAGGGTACCA AGGATGTTAC CATTGCTGGA
    GCGGACTTGT TTTCTGGTTA TAACAAGGAC AACAAGAACT GCGCCTCCAA CAACTCCTGC
    CAGAACACGG CAATAGCTGC GGACGACAAC CAAAACCTGC AGCTCGGTAA CGTCGTCTCG
    GTCGGCGCCA ACAACACCAT TGTATCAGAC AAGAAGGCCA TCCCGGCCAA GGACAACGTC
    GCCACCAACG CGAACCCAAA GTGGTCCCAG ATCAACTCGG TCGCTGCTGT ACAATCTGCC
    CGCGACGCGG GGTCCTGCCG CAACATCGAT GCCTGGCCGG CCAAGCCCGG CGATGGCAAA
    TACTTTGACC CGATCAAGTC CAAACTGCCC ATGCCGTACC CCAAGATGTT TATCAACATG
    GTCAACGGCT CGCCGCTCCG CTTCAACAAC ATCCAGAAGA ACAGCTACCA GATCAAGCCG
    TTCGACTTTG CCGACGTCGA CCCCGGCTTC AGCCCGCAGG TGTCGCTGGA GTACGGACCC
    GGCACCTTCA CCGACTGCAA CGGCGAGGCT GGGTATCAGC TCAACGGGAC GGGCAGCAAG
    TTCCAGATCC GGGCGACGAC AAACGACAAG GACAAGGACA ACAAGATGCG CGCGCAGTAC
    AAGTTCGACT TCCCCGTCAG CGGCGTCCAG CAGGGCGCCG TCTACGAAAT GAGCTCGCGC
    GGCAACGACC GGGCCGTCAA CTTTGTGCTG ACGGGCAGCG ACAAGGTCGG GTACTGGAGC
    AGCCTCAACC CGCCGGTGGC GTGGATGACG CAGCTGCTCC CCGTCATGGG CGGGCGCAAG
    CTCCGCCACA TCAGCATGCT GGGGTCGCAC AACGCGGGCA TCTCGGTCCG GACGGGCGGC
    ACCATCGGGC CCTCGGACGC GTCCATCTGC CAGGGCGTCG ACGTGCTCGG GCAGCTGCAG
    GCCGGGGCGC GGTGGTTCGA CGTGCGGCCC GTCATCGGCA ACGGCGGACA GCTGCTGACG
    GGCCACTACA GCGGGCAAAA GGCGCCCGTG TTTGGCATCA GCGGCCAGGC CATCGACGAC
    ATAGTCGGCA ACGTCAACGC CTTCACCCGC GACAACGCCG AGCTCGTGGT GCTGTCGCTG
    ACGCACGCGA TGCAGACGGA CGAAAAGTAC CGCGACCTCG ACGACGGCGA GTGGGACCGC
    GTGTTCGACG CCATGGAGAA GCTCGACAAC CGCTGCGGCG GGCTCAAGGG CCAGATCCCC
    GACATGAAGC TCGGCGATCT GATCGGCAAG GGCAGGGCGT GCGTGATCGT TCTCGCCGAC
    GGCGGCAAGG TGCGGCCCGA CAAGGGCATC TACCGGCCCG GCGACAGCTT CGACTTCATC
    GAGCGCTGGA GCAACGTCGA CAAGGCCGCC GACATGGCCG CCGACCAGGA GTCGTGGGTG
    CGCGCGAACC GGAACCTGGT CGCCGACGAC AAGGCCCGCA AGGACAAGTT TGCCATCAGC
    CAGTGGATCC TGACAGTCAA GTCGGTGTCG GTTCTGACGT CCAGCATCGA GGACTATGCG
    CTGAACCGCG CCTACGACCC CGTGTTCTGG AGGGGCTACT CGAGCTGGAG CCCGTGGAGC
    TACCCCAGCG TGGTGCTCAT GGACTTTGTG GGCATCATCA ACAGGGGAGA CAAGAAGCTG
    ACTGACATGA GTGGGGAGGT TAGGGCGTTG ATCATGGCGG TCAATCTCGG CTTGGCTAGT
    AGAAACTGTT GGGTTGGGGG CGGTGGTTTG GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003717513.1
    CDS287..4582
    Translation

    Target ORF information:

    RefSeq Version XM_003717465.1
    Organism Pyricularia oryzae 70-15
    Definition Pyricularia oryzae 70-15 glucan 1,3-beta-glucosidase (MGG_09995), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003717465.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGTACG GAACCCTCAT GTTCTGGGCA CTATGCCTAG TGCCCCACCA CGCCTTTGCG 
    GTAAATAATA TCGACAGTGA ACAGTATGCG GTTGGGTCGG AGGCTTGGAA ACGAGCAGGA
    AACCACTTGA ACGAGCACAT CCCGGACCTG GCCCTGCATA AACGCCAGAT ACGAACCGCC
    GATGCGATCC CCCTCCGCGT CAGGAGCGCA ACGCAATCGG ATATCCATGG CGCTCGGGAT
    ATCGTCCAGG CCGCCATGGC CGAAGCCGGT GTGCGCAACC AGAAGCGCAT GCTGAACCCG
    GCTCGCAACA CATACTATGC CGACCAAATA TTCGACTCGA CGCTCCAGAA GCGCGATTAC
    ATGGTTCCAC AGCTGCCCAA GGTTTCCGAC GAAGCTGCTG CTGCCGCTGC GCTCTTGGCC
    GAGGTAGACT CGCTTCAGTC TCGCCGATCG CTAGCCAAGA GGGACGGCAA CGACAAGAGC
    AAGTTCTGGA TGGAGAACAT TGCGCGCAAG GGTTCCATGC CTTGGGGCGA TGATCCGGAC
    TACCAGGTCT TTCGCAACGT CAAGGACTTT GGCGCCAAGG GCGATGGCAT TCACGATGAC
    ACCTTTGCGA TCCGACGTGC CATCCTCGCC GCATCTGGCA AGAAGCCTGA AGTAAACGCC
    CGCTGCACGG GAGATTGCGC TGGCTCCACG ACGCAGAACG CCATTGTGTA CTTTCCTTCA
    GGCACTTACC TTGTCAGCGG CACGATTCCA GCTTTGCCAG CGACGCAGCT CATCGGCGAT
    GCCACCAACC CGCCTACCCT GAAGGCTGCC CCGTCGTTTC TTGGGCTGGG AGTCATCTCG
    ACAAAGGAGT TCCGCGACGA CACCAACGTA ACCAAGATCA AGGACAAGCG AGACGACTCG
    AACGAGGTTT CCACGGCGGC TGGCGATAGC TTTTATCGCC AAATCAGAAA CTTGATTGTC
    GACATCACCT CGGCACCCCA GGACGGCTAC ATCAGTGGCA TCCACTACCA CACATCGCAA
    GCGGCCTCGC TCTCATTTGT GGAGATTCAC GCAACCAGCA TCAAAAGCAA TCCCAACACG
    ACACAAATAG GAATCTTCTC CGAGAATGGC AGCGGAGGTC AAATCAGCGA TCTGACCGTC
    GTGGGAGGAA AAGTCGGCTT CTATGGAGGA AACTCGCAGT TCACCGCTCA GCGCATCAAG
    TTCCGCGAGT GTGGCACCGC AGTTCGGCTT CTCTGGGACT GGAGCTGGGT ATGGAAGAGC
    GTGGAGGTTG CCAACTGCAA CACGGGATTC CAGCTCCACG GCGGCGACAC CTTGAACAGC
    GAGAGTGAAC CCGGTGGCTA TCTGGGATCC ATCCACGTCA TGGACTCGAC CTTTGACAAT
    GTCGGCACCG CTGTCCGCAA TGCGCCCGCG TCGCACGAGC CTGGCGAGGA GACCACTAGC
    ATCACCCTCG ACAATGTTGC CTTTACCGGG CGGGTGGAAC ATCGCGTCTG GGACGGAGAG
    AAAGAGTACG TGCAGGGAAC GCCCAGCAAG CTCGACACGT GGACTTTGGG CGCTCTGTAT
    CTGGATCCTT CCAAGCGTGA CCTGTCTCTG GGTTTTGAGC ACGAGACTCG TCGTGAAGAC
    GGCCTCACTG GAAACAAGAC GAACCCCTCG TTGCCCAAAA AGGCCTACTT TGAGCGTTCC
    AAGCCCCAGT ACACCGATGT CCCCGCCCAA AATTGGGTGC ATACCAAGGA CTCGTGCAAG
    GGAGACGGCA AGGCAGACGA CACAGCGTGT CTGCAGAAGG TCCTGGACAA GAAAGGTTAC
    ATTTTTGTCG ATGCGGGCGT CTATCTGCTA TCCAATACCG TCACCGTCCA CCCGGGCACT
    TTCATCGTCG GCGAGGCATG GTCACAGCTC GTCGCTCAGG GCAACAAGTT CTCCGACGCC
    GGCCAGCCAC GACCCATGAT CCGTGTCGGA AGCAAAGGCG ACAAGGGCTC GGTGGAGATG
    CAGGACCTGC TCTTTACGAC CAAGGGCGCC ACATCCGGCG CCATCCTGGT CGAGTGGAAC
    ATCAAGGCCG AAGGGGAGGG CAAAGCCGGC ATGTGGGACT GCCACGCCCG CGTCGGCGGC
    GCCAGAGGCA CCGAGCTGAC GCCCAAGGAG TGCCCCGCGT CGCCCTCTGG CGGCAGCAGG
    CGACAGTGCC ACGCCGCCGC CCTTCTGATG CACATCACTC CCGGCGCGTC GGCCTACCTC
    GAGAACGTCT GGCTGTGGGC CGCGGACCAC ATGGTTGACG GCGCCTTGCC CGGCCGAGCC
    GAGCGCACCT CGGTGTACGC GGCTCGTGGC ATGCTCATCG AGAGCAGCGA TCCGACGTGG
    CTCTACGGCA CGTCAGCGGA GCACGCTGCC ATGTACCAGT ACAACTTTTA CAGGAGCAAA
    AAGGTCTTTG CGGCCATGAT CAGAACCGAG GCGCCGTTTT TCCAGGATGA GAGGCCGATG
    TTGGTCCAGA GGGGTATCAG TTCGCTGTTT TCGGGGGATC CGATCTGCAC CGCCGCGGAT
    CTTTGCGGAA AGGCTCTGGG CGCCATCGTC AAGGGTACCA AGGATGTTAC CATTGCTGGA
    GCGGACTTGT TTTCTGGTTA TAACAAGGAC AACAAGAACT GCGCCTCCAA CAACTCCTGC
    CAGAACACGG CAATAGCTGC GGACGACAAC CAAAACCTGC AGCTCGGTAA CGTCGTCTCG
    GTCGGCGCCA ACAACACCAT TGTATCAGAC AAGAAGGCCA TCCCGGCCAA GGACAACGTC
    GCCACCAACG CGAACCCAAA GTGGTCCCAG ATCAACTCGG TCGCTGCTGT ACAATCTGCC
    CGCGACGCGG GGTCCTGCCG CAACATCGAT GCCTGGCCGG CCAAGCCCGG CGATGGCAAA
    TACTTTGACC CGATCAAGTC CAAACTGCCC ATGCCGTACC CCAAGATGTT TATCAACATG
    GTCAACGGCT CGCCGCTCCG CTTCAACAAC ATCCAGAAGA ACAGCTACCA GATCAAGCCG
    TTCGACTTTG CCGACGTCGA CCCCGGCTTC AGCCCGCAGG TGTCGCTGGA GTACGGACCC
    GGCACCTTCA CCGACTGCAA CGGCGAGGCT GGGTATCAGC TCAACGGGAC GGGCAGCAAG
    TTCCAGATCC GGGCGACGAC AAACGACAAG GACAAGGACA ACAAGATGCG CGCGCAGTAC
    AAGTTCGACT TCCCCGTCAG CGGCGTCCAG CAGGGCGCCG TCTACGAAAT GAGCTCGCGC
    GGCAACGACC GGGCCGTCAA CTTTGTGCTG ACGGGCAGCG ACAAGGTCGG GTACTGGAGC
    AGCCTCAACC CGCCGGTGGC GTGGATGACG CAGCTGCTCC CCGTCATGGG CGGGCGCAAG
    CTCCGCCACA TCAGCATGCT GGGGTCGCAC AACGCGGGCA TCTCGGTCCG GACGGGCGGC
    ACCATCGGGC CCTCGGACGC GTCCATCTGC CAGGGCGTCG ACGTGCTCGG GCAGCTGCAG
    GCCGGGGCGC GGTGGTTCGA CGTGCGGCCC GTCATCGGCA ACGGCGGACA GCTGCTGACG
    GGCCACTACA GCGGGCAAAA GGCGCCCGTG TTTGGCATCA GCGGCCAGGC CATCGACGAC
    ATAGTCGGCA ACGTCAACGC CTTCACCCGC GACAACGCCG AGCTCGTGGT GCTGTCGCTG
    ACGCACGCGA TGCAGACGGA CGAAAAGTAC CGCGACCTCG ACGACGGCGA GTGGGACCGC
    GTGTTCGACG CCATGGAGAA GCTCGACAAC CGCTGCGGCG GGCTCAAGGG CCAGATCCCC
    GACATGAAGC TCGGCGATCT GATCGGCAAG GGCAGGGCGT GCGTGATCGT TCTCGCCGAC
    GGCGGCAAGG TGCGGCCCGA CAAGGGCATC TACCGGCCCG GCGACAGCTT CGACTTCATC
    GAGCGCTGGA GCAACGTCGA CAAGGCCGCC GACATGGCCG CCGACCAGGA GTCGTGGGTG
    CGCGCGAACC GGAACCTGGT CGCCGACGAC AAGGCCCGCA AGGACAAGTT TGCCATCAGC
    CAGTGGATCC TGACAGTCAA GTCGGTGTCG GTTCTGACGT CCAGCATCGA GGACTATGCG
    CTGAACCGCG CCTACGACCC CGTGTTCTGG AGGGGCTACT CGAGCTGGAG CCCGTGGAGC
    TACCCCAGCG TGGTGCTCAT GGACTTTGTG GGCATCATCA ACAGGGGAGA CAAGAAGCTG
    ACTGACATGA GTGGGGAGGT TAGGGCGTTG ATCATGGCGG TCAATCTCGG CTTGGCTAGT
    AGAAACTGTT GGGTTGGGGG CGGTGGTTTG GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the rice blast fungus Magnaporthe grisea.
    Nature434(7036)980-6(2005 Apr)
    Dean RA,Talbot NJ,Ebbole DJ,Farman ML,Mitchell TK,Orbach MJ,Thon M,Kulkarni R,Xu JR,Pan H,Read ND,Lee YH,Carbone I,Brown D,Oh YY,Donofrio N,Jeong JS,Soanes DM,Djonovic S,Kolomiets E,Rehmeyer C,Li W,Harding M,Kim S,Lebrun MH,Bohnert H,Coughlan S,Butler J,Calvo S,Ma LJ,Nicol R,Purcell S,Nusbaum C,Galagan JE,Birren BW