HELB cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	HELB
Entrez Gene ID	105301236
Full Name	DNA helicase B
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011373817.2	XP_011372119.1	DNA helicase B
XM_011373817.1	XP_011372119.1	DNA helicase B

Pathways from BioSystems
Gene Ontology

The following HELB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HELB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt41574	XM_011373817.2 Latest version!	Pteropus vampyrus DNA helicase B (HELB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OPt41574	XM_011373817.1	Pteropus vampyrus helicase (DNA) B (HELB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HELB ( XM_011373817.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011372119.1 Pteropus vampyrus DNA helicase B (HELB), mRNA.

Price & Availability↑

CloneID	OPt41574
Clone ID Related Accession (Same CDS sequence)	XM_011373817.2 , XM_011373817.1
Accession Version	XM_011373817.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-18
Organism	Pteropus vampyrus(large flying fox)
Product	DNA helicase B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888790.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011373817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCT TGAAACCGCT GTTGCGCGAA CTGCAGGGAC CTCTGCTCCC ACTCAAGGAC 
CTGGTGGAGG AGAGCGATGA CTACTTGGAG GAGGATGAGG AGGATGAAGA GCCGGTGTTC 
GTCGATGCCG AGGAGCTCTG CAGTGGAGGC CTAAAGGCTG GCAGCCTCCC TGGGCGCCTC 
TGGGTTGTGA TTTTTGATGA AAACACTCAA GAGAAGTGTG AAGTGCTTGG ACGTTTTCCA 
TTAACGGGTC CTTGGTGGCG AGTGAAGGTA CAAGTAAAGC CCGTGAGATC AAGGAACTAT 
CAAGTTCAAG GATTTCCATC TTACTTTTTA CAATCTGATA TGTCACCACC AGATCAAAAA 
CATATCTGTT CCCTCTTTCT TAAAGACTGT AATGTCCCCA GTGATCATAT AAATAAATTT 
TTAGCTTGGG TATCTAAAGT ATCAAGCTTT AAAGACCTCA ACTTTGAAAA TCTTTGGGAA 
ACATTAAGAA CTTTCTACAA AGAACTTGGG AGGAATGGTC AAAAACAATC GACACAGACT 
GAACAGAAAG AGTCCCAACC AGATCACGAA ATGTGCCTTC CTGTGGAAAA CACAATTCCA 
TTTATAAATG TAATGACAGC GTTGCGGTTT CCAAAAATAA TGGAATTCCT TCCAGTTCTT 
CTGCCTCGAC ACTTTGAACA GCTAATAAGC TCAGGTTCCA AAGAGGTGTT GGAAAAGATA 
GAAGAGATTT TAGGTGCATG TCCGTGGAAA CTTGGGTTTA GTAAAATAAC CTACAGAGAA 
CTGAAGCTTT TGCGATGTGA GGCGAGTTGG ACAGCGTTTT GTGAGTGCAA GTCTCTTATC 
CAGTTGATGA CTGATTTAGA GAAGAATGCA TTAATAATAT ATTCTAAACT GAAACAGATA 
TGTAGAGAGC ATGGGCACAC GTATGTAGAA GAAGCTGATT TAACCTCTCA GTTGTCAGAC 
CAAATGTCTT TTCTTGATGT TTGGATGTCT CTGAAGTTTT TGAAGGATAT CAATGTGGTG 
ACATATAATA AGAGGGCCTG TATTTTTCCT TATGACCTTT ATCAGGCTGA AAGAGACATT 
GCCTCTTCAG TAGGTGACCT GATGATGAGG CCTCCATGGC ATTTACATGT CGATGTCAAA 
AAGGTGCTTG CGTCTATTCA TATCACAAAA CCTGAGAATT CAAGAAGTGA TGATACATTG 
AATGAAAGTA AACCTGATGA AACAAGCTTA GAAACTTCTG CGGACATTCT GAATGCACAG 
GAGAGTGGTG ACCATATTTG GGATACGGGT GAAAATGAAG ATGATGCAGA AATAAGTGAC 
GATCAACTGG ATCAGGATCA GGTGGCTGCT TTGAAAATGA TTTGCTCCAA TGCTGTGACC 
GTCATAAGTG GGAAAGGTGG TTGTGGGAAG ACCACAATTG TTAGTCATCT TTTTAAACAT 
ATAGAGGTAT TGGAAGAAAG AGAAGTGAAA AAAGCTTGTG AGGACTTCGA ACAAGACCAG 
GATGTGCCAG AAGAATGGAT TGCCTTTACC GAGCAAAGTC CACTGAAGGC AGACAAGGCT 
ATAGAAGTTT TACTTACAGC ACCTACAGGG AAAGCAGCTG GCTTACTGAG ACAGAAAACT 
GGTTTTAGTG CTTATACGCT ATGTCAGATC AATTTTAGCT TCTATTTATG GGATAAAAAA 
TCAACCAAAG ACAGTCCATG GAAGTTTTCT TCGGTGAAAG TTCTGGTTGT GGATGAAGGG 
AGTTTGGTAT CTGTAGGGAT CTTCAGATCC GTTTTATATT TATTACTTGC ACACTCCAAA 
CTTTCTAAGC TTATTATCCT CGGTGATATT AGACAGTTAC CCAGCATTGA ACCTGGTAAC 
TTGCTGCAAG ACCTTTTTGA GACTCTTAAG TCAAGAAATT GTGCTATTGA ACTAAAGACA 
AATCATAGAG CAGAATCTGA GCTAATTGTG GACAATGCTA CAAGAATCTC AAAACGCCGA 
TTTCCAAAAT TCGATGCAGA GCTGAACATT TCTGATAATT CGACACTCCC TGTCTCAGTA 
CAAGATAAAA CATTTATTTT TGTCAGACTC CCAGAAAACG ATGCCAGTTC TCAGTCGTCG 
AAAAGTGATC ATCAATCTCA TTTATATTCT GCTGTTACAA CTTTACTTAA AGAAAACGAC 
CTACAATGTG CAGAAACATC ACAATTTATT GCGTTTAGAA GGCAAGACTG TGATATCATC 
AACGACTGCT GTTGCAAGCA CTACACAGGC CACTTAGTCA GAGACCATAA GAATAGACTT 
ATATTTGGAG TCGGTGATAA AATTTGTTGT ACTAAGAATG CATATCTCTC GGACTTACTG 
CCTGAAAATA CCTCCAGCAG TCAGCAAAAT AAAGAACTAG AGGCCAGCAG TGAAGTCTGT 
AATGGTACTG CTCATGGTTT TGCTAATAAT AAGCATGACT TTGAAAGTGG CATTAGACTC 
TGCAATGGAG AAATATTTTT CATAACACAT GATAAAACTG ATATAACTTC TGGAAAGAAA 
AGATTTTTGA CCATTAATAA TAGGGCTGGC CTGGAAGTAA CTGTGGATTT TAGGAAACTA 
ATGCAAAATT GTCGCATAAG ACATGCCTGG GCAAGAACTA TTCACACTTT TCAGGTAAGA 
CTGGACATTT ATGAAGCTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011372119.1
CDS	92..2755
Translation	MASLKPLLRELQGPLLPLKDLVEESDDYLEEDEEDEEPVFVDAEELCSGGLKAGSLPGRLWVVIFDENTQEKCEVLGRFPLTGPWWRVKVQVKPVRSRNYQVQGFPSYFLQSDMSPPDQKHICSLFLKDCNVPSDHINKFLAWVSKVSSFKDLNFENLWETLRTFYKELGRNGQKQSTQTEQKESQPDHEMCLPVENTIPFINVMTALRFPKIMEFLPVLLPRHFEQLISSGSKEVLEKIEEILGACPWKLGFSKITYRELKLLRCEASWTAFCECKSLIQLMTDLEKNALIIYSKLKQICREHGHTYVEEADLTSQLSDQMSFLDVWMSLKFLKDINVVTYNKRACIFPYDLYQAERDIASSVGDLMMRPPWHLHVDVKKVLASIHITKPENSRSDDTLNESKPDETSLETSADILNAQESGDHIWDTGENEDDAEISDDQLDQDQVAALKMICSNAVTVISGKGGCGKTTIVSHLFKHIEVLEEREVKKACEDFEQDQDVPEEWIAFTEQSPLKADKAIEVLLTAPTGKAAGLLRQKTGFSAYTLCQINFSFYLWDKKSTKDSPWKFSSVKVLVVDEGSLVSVGIFRSVLYLLLAHSKLSKLIILGDIRQLPSIEPGNLLQDLFETLKSRNCAIELKTNHRAESELIVDNATRISKRRFPKFDAELNISDNSTLPVSVQDKTFIFVRLPENDASSQSSKSDHQSHLYSAVTTLLKENDLQCAETSQFIAFRRQDCDIINDCCCKHYTGHLVRDHKNRLIFGVGDKICCTKNAYLSDLLPENTSSSQQNKELEASSEVCNGTAHGFANNKHDFESGIRLCNGEIFFITHDKTDITSGKKRFLTINNRAGLEVTVDFRKLMQNCRIRHAWARTIHTFQVRLDIYEAV

Target ORF information:

RefSeq Version	XM_011373817.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus DNA helicase B (HELB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011373817.2

ORF Insert Sequence:

ATGGCTTCCT TGAAACCGCT GTTGCGCGAA CTGCAGGGAC CTCTGCTCCC ACTCAAGGAC 
CTGGTGGAGG AGAGCGATGA CTACTTGGAG GAGGATGAGG AGGATGAAGA GCCGGTGTTC 
GTCGATGCCG AGGAGCTCTG CAGTGGAGGC CTAAAGGCTG GCAGCCTCCC TGGGCGCCTC 
TGGGTTGTGA TTTTTGATGA AAACACTCAA GAGAAGTGTG AAGTGCTTGG ACGTTTTCCA 
TTAACGGGTC CTTGGTGGCG AGTGAAGGTA CAAGTAAAGC CCGTGAGATC AAGGAACTAT 
CAAGTTCAAG GATTTCCATC TTACTTTTTA CAATCTGATA TGTCACCACC AGATCAAAAA 
CATATCTGTT CCCTCTTTCT TAAAGACTGT AATGTCCCCA GTGATCATAT AAATAAATTT 
TTAGCTTGGG TATCTAAAGT ATCAAGCTTT AAAGACCTCA ACTTTGAAAA TCTTTGGGAA 
ACATTAAGAA CTTTCTACAA AGAACTTGGG AGGAATGGTC AAAAACAATC GACACAGACT 
GAACAGAAAG AGTCCCAACC AGATCACGAA ATGTGCCTTC CTGTGGAAAA CACAATTCCA 
TTTATAAATG TAATGACAGC GTTGCGGTTT CCAAAAATAA TGGAATTCCT TCCAGTTCTT 
CTGCCTCGAC ACTTTGAACA GCTAATAAGC TCAGGTTCCA AAGAGGTGTT GGAAAAGATA 
GAAGAGATTT TAGGTGCATG TCCGTGGAAA CTTGGGTTTA GTAAAATAAC CTACAGAGAA 
CTGAAGCTTT TGCGATGTGA GGCGAGTTGG ACAGCGTTTT GTGAGTGCAA GTCTCTTATC 
CAGTTGATGA CTGATTTAGA GAAGAATGCA TTAATAATAT ATTCTAAACT GAAACAGATA 
TGTAGAGAGC ATGGGCACAC GTATGTAGAA GAAGCTGATT TAACCTCTCA GTTGTCAGAC 
CAAATGTCTT TTCTTGATGT TTGGATGTCT CTGAAGTTTT TGAAGGATAT CAATGTGGTG 
ACATATAATA AGAGGGCCTG TATTTTTCCT TATGACCTTT ATCAGGCTGA AAGAGACATT 
GCCTCTTCAG TAGGTGACCT GATGATGAGG CCTCCATGGC ATTTACATGT CGATGTCAAA 
AAGGTGCTTG CGTCTATTCA TATCACAAAA CCTGAGAATT CAAGAAGTGA TGATACATTG 
AATGAAAGTA AACCTGATGA AACAAGCTTA GAAACTTCTG CGGACATTCT GAATGCACAG 
GAGAGTGGTG ACCATATTTG GGATACGGGT GAAAATGAAG ATGATGCAGA AATAAGTGAC 
GATCAACTGG ATCAGGATCA GGTGGCTGCT TTGAAAATGA TTTGCTCCAA TGCTGTGACC 
GTCATAAGTG GGAAAGGTGG TTGTGGGAAG ACCACAATTG TTAGTCATCT TTTTAAACAT 
ATAGAGGTAT TGGAAGAAAG AGAAGTGAAA AAAGCTTGTG AGGACTTCGA ACAAGACCAG 
GATGTGCCAG AAGAATGGAT TGCCTTTACC GAGCAAAGTC CACTGAAGGC AGACAAGGCT 
ATAGAAGTTT TACTTACAGC ACCTACAGGG AAAGCAGCTG GCTTACTGAG ACAGAAAACT 
GGTTTTAGTG CTTATACGCT ATGTCAGATC AATTTTAGCT TCTATTTATG GGATAAAAAA 
TCAACCAAAG ACAGTCCATG GAAGTTTTCT TCGGTGAAAG TTCTGGTTGT GGATGAAGGG 
AGTTTGGTAT CTGTAGGGAT CTTCAGATCC GTTTTATATT TATTACTTGC ACACTCCAAA 
CTTTCTAAGC TTATTATCCT CGGTGATATT AGACAGTTAC CCAGCATTGA ACCTGGTAAC 
TTGCTGCAAG ACCTTTTTGA GACTCTTAAG TCAAGAAATT GTGCTATTGA ACTAAAGACA 
AATCATAGAG CAGAATCTGA GCTAATTGTG GACAATGCTA CAAGAATCTC AAAACGCCGA 
TTTCCAAAAT TCGATGCAGA GCTGAACATT TCTGATAATT CGACACTCCC TGTCTCAGTA 
CAAGATAAAA CATTTATTTT TGTCAGACTC CCAGAAAACG ATGCCAGTTC TCAGTCGTCG 
AAAAGTGATC ATCAATCTCA TTTATATTCT GCTGTTACAA CTTTACTTAA AGAAAACGAC 
CTACAATGTG CAGAAACATC ACAATTTATT GCGTTTAGAA GGCAAGACTG TGATATCATC 
AACGACTGCT GTTGCAAGCA CTACACAGGC CACTTAGTCA GAGACCATAA GAATAGACTT 
ATATTTGGAG TCGGTGATAA AATTTGTTGT ACTAAGAATG CATATCTCTC GGACTTACTG 
CCTGAAAATA CCTCCAGCAG TCAGCAAAAT AAAGAACTAG AGGCCAGCAG TGAAGTCTGT 
AATGGTACTG CTCATGGTTT TGCTAATAAT AAGCATGACT TTGAAAGTGG CATTAGACTC 
TGCAATGGAG AAATATTTTT CATAACACAT GATAAAACTG ATATAACTTC TGGAAAGAAA 
AGATTTTTGA CCATTAATAA TAGGGCTGGC CTGGAAGTAA CTGTGGATTT TAGGAAACTA 
ATGCAAAATT GTCGCATAAG ACATGCCTGG GCAAGAACTA TTCACACTTT TCAGGTAAGA 
CTGGACATTT ATGAAGCTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HELB ( XM_011373817.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011372119.1 Pteropus vampyrus helicase (DNA) B (HELB), mRNA.

Price & Availability↑

CloneID	OPt41574
Clone ID Related Accession (Same CDS sequence)	XM_011373817.2 , XM_011373817.1
Accession Version	XM_011373817.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-22
Organism	Pteropus vampyrus(large flying fox)
Product	DNA helicase B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888790.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCT TGAAACCGCT GTTGCGCGAA CTGCAGGGAC CTCTGCTCCC ACTCAAGGAC 
CTGGTGGAGG AGAGCGATGA CTACTTGGAG GAGGATGAGG AGGATGAAGA GCCGGTGTTC 
GTCGATGCCG AGGAGCTCTG CAGTGGAGGC CTAAAGGCTG GCAGCCTCCC TGGGCGCCTC 
TGGGTTGTGA TTTTTGATGA AAACACTCAA GAGAAGTGTG AAGTGCTTGG ACGTTTTCCA 
TTAACGGGTC CTTGGTGGCG AGTGAAGGTA CAAGTAAAGC CCGTGAGATC AAGGAACTAT 
CAAGTTCAAG GATTTCCATC TTACTTTTTA CAATCTGATA TGTCACCACC AGATCAAAAA 
CATATCTGTT CCCTCTTTCT TAAAGACTGT AATGTCCCCA GTGATCATAT AAATAAATTT 
TTAGCTTGGG TATCTAAAGT ATCAAGCTTT AAAGACCTCA ACTTTGAAAA TCTTTGGGAA 
ACATTAAGAA CTTTCTACAA AGAACTTGGG AGGAATGGTC AAAAACAATC GACACAGACT 
GAACAGAAAG AGTCCCAACC AGATCACGAA ATGTGCCTTC CTGTGGAAAA CACAATTCCA 
TTTATAAATG TAATGACAGC GTTGCGGTTT CCAAAAATAA TGGAATTCCT TCCAGTTCTT 
CTGCCTCGAC ACTTTGAACA GCTAATAAGC TCAGGTTCCA AAGAGGTGTT GGAAAAGATA 
GAAGAGATTT TAGGTGCATG TCCGTGGAAA CTTGGGTTTA GTAAAATAAC CTACAGAGAA 
CTGAAGCTTT TGCGATGTGA GGCGAGTTGG ACAGCGTTTT GTGAGTGCAA GTCTCTTATC 
CAGTTGATGA CTGATTTAGA GAAGAATGCA TTAATAATAT ATTCTAAACT GAAACAGATA 
TGTAGAGAGC ATGGGCACAC GTATGTAGAA GAAGCTGATT TAACCTCTCA GTTGTCAGAC 
CAAATGTCTT TTCTTGATGT TTGGATGTCT CTGAAGTTTT TGAAGGATAT CAATGTGGTG 
ACATATAATA AGAGGGCCTG TATTTTTCCT TATGACCTTT ATCAGGCTGA AAGAGACATT 
GCCTCTTCAG TAGGTGACCT GATGATGAGG CCTCCATGGC ATTTACATGT CGATGTCAAA 
AAGGTGCTTG CGTCTATTCA TATCACAAAA CCTGAGAATT CAAGAAGTGA TGATACATTG 
AATGAAAGTA AACCTGATGA AACAAGCTTA GAAACTTCTG CGGACATTCT GAATGCACAG 
GAGAGTGGTG ACCATATTTG GGATACGGGT GAAAATGAAG ATGATGCAGA AATAAGTGAC 
GATCAACTGG ATCAGGATCA GGTGGCTGCT TTGAAAATGA TTTGCTCCAA TGCTGTGACC 
GTCATAAGTG GGAAAGGTGG TTGTGGGAAG ACCACAATTG TTAGTCATCT TTTTAAACAT 
ATAGAGGTAT TGGAAGAAAG AGAAGTGAAA AAAGCTTGTG AGGACTTCGA ACAAGACCAG 
GATGTGCCAG AAGAATGGAT TGCCTTTACC GAGCAAAGTC CACTGAAGGC AGACAAGGCT 
ATAGAAGTTT TACTTACAGC ACCTACAGGG AAAGCAGCTG GCTTACTGAG ACAGAAAACT 
GGTTTTAGTG CTTATACGCT ATGTCAGATC AATTTTAGCT TCTATTTATG GGATAAAAAA 
TCAACCAAAG ACAGTCCATG GAAGTTTTCT TCGGTGAAAG TTCTGGTTGT GGATGAAGGG 
AGTTTGGTAT CTGTAGGGAT CTTCAGATCC GTTTTATATT TATTACTTGC ACACTCCAAA 
CTTTCTAAGC TTATTATCCT CGGTGATATT AGACAGTTAC CCAGCATTGA ACCTGGTAAC 
TTGCTGCAAG ACCTTTTTGA GACTCTTAAG TCAAGAAATT GTGCTATTGA ACTAAAGACA 
AATCATAGAG CAGAATCTGA GCTAATTGTG GACAATGCTA CAAGAATCTC AAAACGCCGA 
TTTCCAAAAT TCGATGCAGA GCTGAACATT TCTGATAATT CGACACTCCC TGTCTCAGTA 
CAAGATAAAA CATTTATTTT TGTCAGACTC CCAGAAAACG ATGCCAGTTC TCAGTCGTCG 
AAAAGTGATC ATCAATCTCA TTTATATTCT GCTGTTACAA CTTTACTTAA AGAAAACGAC 
CTACAATGTG CAGAAACATC ACAATTTATT GCGTTTAGAA GGCAAGACTG TGATATCATC 
AACGACTGCT GTTGCAAGCA CTACACAGGC CACTTAGTCA GAGACCATAA GAATAGACTT 
ATATTTGGAG TCGGTGATAA AATTTGTTGT ACTAAGAATG CATATCTCTC GGACTTACTG 
CCTGAAAATA CCTCCAGCAG TCAGCAAAAT AAAGAACTAG AGGCCAGCAG TGAAGTCTGT 
AATGGTACTG CTCATGGTTT TGCTAATAAT AAGCATGACT TTGAAAGTGG CATTAGACTC 
TGCAATGGAG AAATATTTTT CATAACACAT GATAAAACTG ATATAACTTC TGGAAAGAAA 
AGATTTTTGA CCATTAATAA TAGGGCTGGC CTGGAAGTAA CTGTGGATTT TAGGAAACTA 
ATGCAAAATT GTCGCATAAG ACATGCCTGG GCAAGAACTA TTCACACTTT TCAGGTAAGA 
CTGGACATTT ATGAAGCTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011372119.1
CDS	82..2745
Translation	MASLKPLLRELQGPLLPLKDLVEESDDYLEEDEEDEEPVFVDAEELCSGGLKAGSLPGRLWVVIFDENTQEKCEVLGRFPLTGPWWRVKVQVKPVRSRNYQVQGFPSYFLQSDMSPPDQKHICSLFLKDCNVPSDHINKFLAWVSKVSSFKDLNFENLWETLRTFYKELGRNGQKQSTQTEQKESQPDHEMCLPVENTIPFINVMTALRFPKIMEFLPVLLPRHFEQLISSGSKEVLEKIEEILGACPWKLGFSKITYRELKLLRCEASWTAFCECKSLIQLMTDLEKNALIIYSKLKQICREHGHTYVEEADLTSQLSDQMSFLDVWMSLKFLKDINVVTYNKRACIFPYDLYQAERDIASSVGDLMMRPPWHLHVDVKKVLASIHITKPENSRSDDTLNESKPDETSLETSADILNAQESGDHIWDTGENEDDAEISDDQLDQDQVAALKMICSNAVTVISGKGGCGKTTIVSHLFKHIEVLEEREVKKACEDFEQDQDVPEEWIAFTEQSPLKADKAIEVLLTAPTGKAAGLLRQKTGFSAYTLCQINFSFYLWDKKSTKDSPWKFSSVKVLVVDEGSLVSVGIFRSVLYLLLAHSKLSKLIILGDIRQLPSIEPGNLLQDLFETLKSRNCAIELKTNHRAESELIVDNATRISKRRFPKFDAELNISDNSTLPVSVQDKTFIFVRLPENDASSQSSKSDHQSHLYSAVTTLLKENDLQCAETSQFIAFRRQDCDIINDCCCKHYTGHLVRDHKNRLIFGVGDKICCTKNAYLSDLLPENTSSSQQNKELEASSEVCNGTAHGFANNKHDFESGIRLCNGEIFFITHDKTDITSGKKRFLTINNRAGLEVTVDFRKLMQNCRIRHAWARTIHTFQVRLDIYEAV

Target ORF information:

RefSeq Version	XM_011373817.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus helicase (DNA) B (HELB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011373817.1

ORF Insert Sequence:

ATGGCTTCCT TGAAACCGCT GTTGCGCGAA CTGCAGGGAC CTCTGCTCCC ACTCAAGGAC 
CTGGTGGAGG AGAGCGATGA CTACTTGGAG GAGGATGAGG AGGATGAAGA GCCGGTGTTC 
GTCGATGCCG AGGAGCTCTG CAGTGGAGGC CTAAAGGCTG GCAGCCTCCC TGGGCGCCTC 
TGGGTTGTGA TTTTTGATGA AAACACTCAA GAGAAGTGTG AAGTGCTTGG ACGTTTTCCA 
TTAACGGGTC CTTGGTGGCG AGTGAAGGTA CAAGTAAAGC CCGTGAGATC AAGGAACTAT 
CAAGTTCAAG GATTTCCATC TTACTTTTTA CAATCTGATA TGTCACCACC AGATCAAAAA 
CATATCTGTT CCCTCTTTCT TAAAGACTGT AATGTCCCCA GTGATCATAT AAATAAATTT 
TTAGCTTGGG TATCTAAAGT ATCAAGCTTT AAAGACCTCA ACTTTGAAAA TCTTTGGGAA 
ACATTAAGAA CTTTCTACAA AGAACTTGGG AGGAATGGTC AAAAACAATC GACACAGACT 
GAACAGAAAG AGTCCCAACC AGATCACGAA ATGTGCCTTC CTGTGGAAAA CACAATTCCA 
TTTATAAATG TAATGACAGC GTTGCGGTTT CCAAAAATAA TGGAATTCCT TCCAGTTCTT 
CTGCCTCGAC ACTTTGAACA GCTAATAAGC TCAGGTTCCA AAGAGGTGTT GGAAAAGATA 
GAAGAGATTT TAGGTGCATG TCCGTGGAAA CTTGGGTTTA GTAAAATAAC CTACAGAGAA 
CTGAAGCTTT TGCGATGTGA GGCGAGTTGG ACAGCGTTTT GTGAGTGCAA GTCTCTTATC 
CAGTTGATGA CTGATTTAGA GAAGAATGCA TTAATAATAT ATTCTAAACT GAAACAGATA 
TGTAGAGAGC ATGGGCACAC GTATGTAGAA GAAGCTGATT TAACCTCTCA GTTGTCAGAC 
CAAATGTCTT TTCTTGATGT TTGGATGTCT CTGAAGTTTT TGAAGGATAT CAATGTGGTG 
ACATATAATA AGAGGGCCTG TATTTTTCCT TATGACCTTT ATCAGGCTGA AAGAGACATT 
GCCTCTTCAG TAGGTGACCT GATGATGAGG CCTCCATGGC ATTTACATGT CGATGTCAAA 
AAGGTGCTTG CGTCTATTCA TATCACAAAA CCTGAGAATT CAAGAAGTGA TGATACATTG 
AATGAAAGTA AACCTGATGA AACAAGCTTA GAAACTTCTG CGGACATTCT GAATGCACAG 
GAGAGTGGTG ACCATATTTG GGATACGGGT GAAAATGAAG ATGATGCAGA AATAAGTGAC 
GATCAACTGG ATCAGGATCA GGTGGCTGCT TTGAAAATGA TTTGCTCCAA TGCTGTGACC 
GTCATAAGTG GGAAAGGTGG TTGTGGGAAG ACCACAATTG TTAGTCATCT TTTTAAACAT 
ATAGAGGTAT TGGAAGAAAG AGAAGTGAAA AAAGCTTGTG AGGACTTCGA ACAAGACCAG 
GATGTGCCAG AAGAATGGAT TGCCTTTACC GAGCAAAGTC CACTGAAGGC AGACAAGGCT 
ATAGAAGTTT TACTTACAGC ACCTACAGGG AAAGCAGCTG GCTTACTGAG ACAGAAAACT 
GGTTTTAGTG CTTATACGCT ATGTCAGATC AATTTTAGCT TCTATTTATG GGATAAAAAA 
TCAACCAAAG ACAGTCCATG GAAGTTTTCT TCGGTGAAAG TTCTGGTTGT GGATGAAGGG 
AGTTTGGTAT CTGTAGGGAT CTTCAGATCC GTTTTATATT TATTACTTGC ACACTCCAAA 
CTTTCTAAGC TTATTATCCT CGGTGATATT AGACAGTTAC CCAGCATTGA ACCTGGTAAC 
TTGCTGCAAG ACCTTTTTGA GACTCTTAAG TCAAGAAATT GTGCTATTGA ACTAAAGACA 
AATCATAGAG CAGAATCTGA GCTAATTGTG GACAATGCTA CAAGAATCTC AAAACGCCGA 
TTTCCAAAAT TCGATGCAGA GCTGAACATT TCTGATAATT CGACACTCCC TGTCTCAGTA 
CAAGATAAAA CATTTATTTT TGTCAGACTC CCAGAAAACG ATGCCAGTTC TCAGTCGTCG 
AAAAGTGATC ATCAATCTCA TTTATATTCT GCTGTTACAA CTTTACTTAA AGAAAACGAC 
CTACAATGTG CAGAAACATC ACAATTTATT GCGTTTAGAA GGCAAGACTG TGATATCATC 
AACGACTGCT GTTGCAAGCA CTACACAGGC CACTTAGTCA GAGACCATAA GAATAGACTT 
ATATTTGGAG TCGGTGATAA AATTTGTTGT ACTAAGAATG CATATCTCTC GGACTTACTG 
CCTGAAAATA CCTCCAGCAG TCAGCAAAAT AAAGAACTAG AGGCCAGCAG TGAAGTCTGT 
AATGGTACTG CTCATGGTTT TGCTAATAAT AAGCATGACT TTGAAAGTGG CATTAGACTC 
TGCAATGGAG AAATATTTTT CATAACACAT GATAAAACTG ATATAACTTC TGGAAAGAAA 
AGATTTTTGA CCATTAATAA TAGGGCTGGC CTGGAAGTAA CTGTGGATTT TAGGAAACTA 
ATGCAAAATT GTCGCATAAG ACATGCCTGG GCAAGAACTA TTCACACTTT TCAGGTAAGA 
CTGGACATTT ATGAAGCTGT TTAA