SLC12A4 cDNA ORF clone, Pteropus alecto(black flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC12A4
Entrez Gene ID	102883553
Full Name	solute carrier family 12 member 4
Gene Type	protein-coding
Organism	Pteropus alecto(black flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006909866.2	XP_006909928.1	solute carrier family 12 member 4
XM_025045252.1	XP_024901020.1	solute carrier family 12 member 4

Pathways from BioSystems
Gene Ontology

The following SLC12A4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPt11943	XM_006909866.2 Latest version!	Pteropus alecto solute carrier family 12 (potassium/chloride transporter), member 4 (SLC12A4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPt85549	XM_025045252.1 Latest version!	Pteropus alecto solute carrier family 12 member 4 (SLC12A4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC12A4 ( XM_006909866.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006909928.1 Pteropus alecto solute carrier family 12 (potassium/chloride transporter), member 4 (SLC12A4), mRNA.

Price & Availability↑

CloneID	OPt11943
Clone ID Related Accession (Same CDS sequence)	XM_006909866.2
Accession Version	XM_006909866.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	solute carrier family 12 member 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006434825.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 10, 2016 this sequence version replaced XM_006909866.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCACT TCACTGTGGT ACCGGTGGAC GGGCCGCGGC GCGGCGACTA TGACAACCTC 
GAGGGGCTCA GTTGGGTGGA CTACGGGGAG CGCGCCGAGC GAGAAGACTC AGACGGACAC 
GGCAACCACA AAGAAAGCAG CCCTTTCCTT TGTCCCTTGG AGGCTTCCAG AGGAAGTGAA 
TACTATGACA GGAACCTGGC ACTGTTTGAG GAAGAGCTGG ACATCCGCCC AAAGGTGTCA 
TCTCTTCTGG GCAAACTCGT CAGCTACACC AATCTCACAC AAGGCGCCAA GGAGCATGAG 
GAGGCTGAAA GTGGGGAGGG CACCCGCCGG AGAGCAGCCA AGGCACCTAG CATGGGCACC 
CTCATGGGGG TGTACCTACC CTGCCTGCAG AATATTTTTG GAGTTATCCT CTTCCTGCGG 
CTGACCTGGA TGGTGGGAAC AGCTGGTGTG CTACAAGCCC TCCTCATTGT TCTCATCTGC 
TGCTGCTGTA CCTTGCTGAC GGCCATCTCC ATGAGCGCCA TCGCCACCAA TGGAGTGGTT 
CCAGCCGGGG GCTCCTATTT CATGATCTCC CGCTCGCTGG GGCCAGAATT TGGAGGTGCT 
GTGGGCCTAT GCTTCTACCT GGGAACAACA TTTGCGGCGG CCATGTACAT CCTGGGGGCC 
ATTGAGATCT TGCTGACTTA CATTGCCCCG CCAGCTGCCA TTTTTTACCC AACAGGCGCT 
CATGATACAT CAAGTGCCAC CTTGAACAAC ATGCGAGTGT ATGGGACCAT TTTTCTAACC 
TTCATGACCC TGGTGGTGTT TGTTGGTGTC AAGTATGTGA ACAAATTTGC CTCACTCTTC 
CTGGCCTGTG TGATCATCTC CATCCTCTCC ATCTATGCTG GGGGCATCAA GTCTATTTTT 
GATCCGCCCA TATTTCCAGT CTGCATGCTG GGCAACAGGA CCCTATCACG GGACCAGTTT 
GACATCTGTG CCAAGACGGC CATAGTGGAT AATGAGACAG TGGCCACACA GCTATGGAGC 
CTCTTCTGCC ACAGCACCAA CCTTACCACT GACTCCTGCG ACTCTTACTT CCTGCTCAAC 
AATGTGACCG AGATCCCTGG CATTCCAGGT GCAGCTGCTG GCGTGCTCCA GGAGAACCTG 
TGGAGCACCT ACCTGGAGAA GGGTGAGGTC GTGGAGAAGC ACGGGCTGCC TTCCACAGAC 
GCCCTTGGCG TGAAGGAGAG CCTGCCCCTA TACGTGGTGG CTGACATTGC CACATCCTTC 
ACTGTGCTGG TCGGCATCTT CTTCCCCTCT GTGACAGGCA TTATGGCTGG CTCAAATCGC 
TCCGGGGACC TCCGCGACGC CCAGAAGTCC ATTCCAGTGG GGACCATTCT GGCCATTGTT 
ACAACTTCCC TTGTGTACTT CAGCAGTGTG GTTCTCTTTG GCGCCTGCAT TGAGGGTGTG 
GTACTCCGGG ACAAAGTGGT GGGCACATTG GCCTGGCCTT CACCCTGGGT CATCGTCATC 
GGCTCCTTCT TCTCAACTTG TGGTGCCGGC CTACAGAGCC TCACTGGGGC ACCACGCCTG 
TTGCAGGCCA TTGCCAAGGA CAACATCATC CCCTTCCTCC GGGTATTTGG CCACGGGAAG 
GCAAATGGTG AACCAACATG GGCACTCCTC CTGACGGCAC TCATCGCTGA GCTGGGCATC 
CTCATTGCCT CCCTCGACAT GGTGGCCCCC ATTCTGTCCA TGTTCTTTCT GATGTGTTAC 
CTGTTTGTGA ACCTGGCCTG TGCTGTGCAG ACGCTCCTGA GGACCCCCAA CTGGCGGCCC 
CGGTTCAAGT ACTATCACTG GGCACTGTCC TTCCTGGGCA TGAGTCTCTG CCTGGCCCTT 
ATGTTTGTCT CCTCCTGGTA CTATGCCCTG GTTGCCATGC TCATTGCGGG CATGATCTAC 
AAGTACATTG AGTACCAAGG GGCTGAGAAG GAATGGGGTG ACGGGATCCG AGGCCTGTCC 
CTGAGTGCTG CCCGTTACGC ATTGTTGAGA TTAGAGGAGG GGCCTCCTCA CACCAAGAAC 
TGGCGGCCTC AGCTGCTGGT GCTGCTGAAG CTAGATGAGG ATCTTCACGT GAAGTACCCA 
CGGCTCCTCA CCTTTGCGTC CCAGCTGAAG GCAGGCAAGG GCTTGACCAT CGTTGGTTCT 
GTCATCCAGG GAAGCTTCTT GGAGAGCTAT GGCGAGGCCC AGGCCGCTGA GCAGACAATC 
AAGAACATGA TGGAGATTGA GAAAGTGAAG GGCTTCTGCC AGGTGGTGGT AGCCAGCAAG 
GTGCGTGAAG GGCTGGCCCA CCTCATCCAG TCTTGCGGCC TAGGCGGCAT GAGGCATAAT 
TCCGTGGTGC TGGGCTGGCC CTATGGCTGG CGACAGAGTG AGGACCCACG TGCCTGGAAG 
ACCTTTATTG ACACCGTTCG TTGCACCACG GCTGCCCACC TGGCCCTGCT TGTGCCCAAG 
AACATCGCCT TCTACCCCAG CAACCATGAG CGCTACCTGG AGGGCCACAT CGATGTGTGG 
TGGATTGTGC ATGATGGTGG CATGCTTATG CTTTTGCCCT TCCTGCTGCG CCAGCACAAG 
GTTTGGAGGA AGTGCCGGAT GCGCATCTTC ACTGTGGCCC AGATGGATGA CAACAGCATC 
CAGATGAAGA AGGATCTGGC CATCTTCCTG TACCATCTAC GCCTTGAGGC AGAGGTAGAG 
GTAGTAGAGA TGCACAACAG CGACATCTCT GCATATACCT ATGAGCGGAC ACTGATGATG 
GAGCAGCGGT CCCAGATGCT GCGGCAGATG AGACTGACCA AGACTGAGCG GGAGCGAGAA 
GCCCAGCTGG TCAAGGACCG GCATTCAGCC TTGCGACTGG AAAGCCTGTA CTCAGACGAG 
GAAGATGAGT CTGCAGCTGG GGCTGACAAG ATCCAGATGA CATGGACACG GGACAAGTAT 
ATGGCAGCTG AACCCTGGGA CCCCAGCCAT GCCCCTGACA ACTTCCGAGA ACTGGTCCAT 
ATTAAGCCGG ACCAGTCCAA TGTGCGGCGC ATGCACACTG CTGTGAAGCT CAATGAAGTC 
ATTGTCACAC GCTCCCATGA TGCCCGATTG GTCCTACTGA ACATGCCTGG CCCACCCAAG 
AACAGCGAGG GCGATGAGAA CTACATGGAG TTCCTGGAGG TACTGACCGA GGGCCTTGAG 
CGGGTGCTGT TGGTGCGTGG CGGTGGCCGT GAAGTCATCA CCATCTACTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006909928.1
CDS	67..3300
Translation	MPHFTVVPVDGPRRGDYDNLEGLSWVDYGERAEREDSDGHGNHKESSPFLCPLEASRGSEYYDRNLALFEEELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGTRRRAAKAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPTGAHDTSSATLNNMRVYGTIFLTFMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPPIFPVCMLGNRTLSRDQFDICAKTAIVDNETVATQLWSLFCHSTNLTTDSCDSYFLLNNVTEIPGIPGAAAGVLQENLWSTYLEKGEVVEKHGLPSTDALGVKESLPLYVVADIATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVVLFGACIEGVVLRDKVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMEIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFYPSNHERYLEGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTEREREAQLVKDRHSALRLESLYSDEEDESAAGADKIQMTWTRDKYMAAEPWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPKNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_006909866.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto solute carrier family 12 (potassium/chloride transporter), member 4 (SLC12A4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006909866.2

ORF Insert Sequence:

ATGCCTCACT TCACTGTGGT ACCGGTGGAC GGGCCGCGGC GCGGCGACTA TGACAACCTC 
GAGGGGCTCA GTTGGGTGGA CTACGGGGAG CGCGCCGAGC GAGAAGACTC AGACGGACAC 
GGCAACCACA AAGAAAGCAG CCCTTTCCTT TGTCCCTTGG AGGCTTCCAG AGGAAGTGAA 
TACTATGACA GGAACCTGGC ACTGTTTGAG GAAGAGCTGG ACATCCGCCC AAAGGTGTCA 
TCTCTTCTGG GCAAACTCGT CAGCTACACC AATCTCACAC AAGGCGCCAA GGAGCATGAG 
GAGGCTGAAA GTGGGGAGGG CACCCGCCGG AGAGCAGCCA AGGCACCTAG CATGGGCACC 
CTCATGGGGG TGTACCTACC CTGCCTGCAG AATATTTTTG GAGTTATCCT CTTCCTGCGG 
CTGACCTGGA TGGTGGGAAC AGCTGGTGTG CTACAAGCCC TCCTCATTGT TCTCATCTGC 
TGCTGCTGTA CCTTGCTGAC GGCCATCTCC ATGAGCGCCA TCGCCACCAA TGGAGTGGTT 
CCAGCCGGGG GCTCCTATTT CATGATCTCC CGCTCGCTGG GGCCAGAATT TGGAGGTGCT 
GTGGGCCTAT GCTTCTACCT GGGAACAACA TTTGCGGCGG CCATGTACAT CCTGGGGGCC 
ATTGAGATCT TGCTGACTTA CATTGCCCCG CCAGCTGCCA TTTTTTACCC AACAGGCGCT 
CATGATACAT CAAGTGCCAC CTTGAACAAC ATGCGAGTGT ATGGGACCAT TTTTCTAACC 
TTCATGACCC TGGTGGTGTT TGTTGGTGTC AAGTATGTGA ACAAATTTGC CTCACTCTTC 
CTGGCCTGTG TGATCATCTC CATCCTCTCC ATCTATGCTG GGGGCATCAA GTCTATTTTT 
GATCCGCCCA TATTTCCAGT CTGCATGCTG GGCAACAGGA CCCTATCACG GGACCAGTTT 
GACATCTGTG CCAAGACGGC CATAGTGGAT AATGAGACAG TGGCCACACA GCTATGGAGC 
CTCTTCTGCC ACAGCACCAA CCTTACCACT GACTCCTGCG ACTCTTACTT CCTGCTCAAC 
AATGTGACCG AGATCCCTGG CATTCCAGGT GCAGCTGCTG GCGTGCTCCA GGAGAACCTG 
TGGAGCACCT ACCTGGAGAA GGGTGAGGTC GTGGAGAAGC ACGGGCTGCC TTCCACAGAC 
GCCCTTGGCG TGAAGGAGAG CCTGCCCCTA TACGTGGTGG CTGACATTGC CACATCCTTC 
ACTGTGCTGG TCGGCATCTT CTTCCCCTCT GTGACAGGCA TTATGGCTGG CTCAAATCGC 
TCCGGGGACC TCCGCGACGC CCAGAAGTCC ATTCCAGTGG GGACCATTCT GGCCATTGTT 
ACAACTTCCC TTGTGTACTT CAGCAGTGTG GTTCTCTTTG GCGCCTGCAT TGAGGGTGTG 
GTACTCCGGG ACAAAGTGGT GGGCACATTG GCCTGGCCTT CACCCTGGGT CATCGTCATC 
GGCTCCTTCT TCTCAACTTG TGGTGCCGGC CTACAGAGCC TCACTGGGGC ACCACGCCTG 
TTGCAGGCCA TTGCCAAGGA CAACATCATC CCCTTCCTCC GGGTATTTGG CCACGGGAAG 
GCAAATGGTG AACCAACATG GGCACTCCTC CTGACGGCAC TCATCGCTGA GCTGGGCATC 
CTCATTGCCT CCCTCGACAT GGTGGCCCCC ATTCTGTCCA TGTTCTTTCT GATGTGTTAC 
CTGTTTGTGA ACCTGGCCTG TGCTGTGCAG ACGCTCCTGA GGACCCCCAA CTGGCGGCCC 
CGGTTCAAGT ACTATCACTG GGCACTGTCC TTCCTGGGCA TGAGTCTCTG CCTGGCCCTT 
ATGTTTGTCT CCTCCTGGTA CTATGCCCTG GTTGCCATGC TCATTGCGGG CATGATCTAC 
AAGTACATTG AGTACCAAGG GGCTGAGAAG GAATGGGGTG ACGGGATCCG AGGCCTGTCC 
CTGAGTGCTG CCCGTTACGC ATTGTTGAGA TTAGAGGAGG GGCCTCCTCA CACCAAGAAC 
TGGCGGCCTC AGCTGCTGGT GCTGCTGAAG CTAGATGAGG ATCTTCACGT GAAGTACCCA 
CGGCTCCTCA CCTTTGCGTC CCAGCTGAAG GCAGGCAAGG GCTTGACCAT CGTTGGTTCT 
GTCATCCAGG GAAGCTTCTT GGAGAGCTAT GGCGAGGCCC AGGCCGCTGA GCAGACAATC 
AAGAACATGA TGGAGATTGA GAAAGTGAAG GGCTTCTGCC AGGTGGTGGT AGCCAGCAAG 
GTGCGTGAAG GGCTGGCCCA CCTCATCCAG TCTTGCGGCC TAGGCGGCAT GAGGCATAAT 
TCCGTGGTGC TGGGCTGGCC CTATGGCTGG CGACAGAGTG AGGACCCACG TGCCTGGAAG 
ACCTTTATTG ACACCGTTCG TTGCACCACG GCTGCCCACC TGGCCCTGCT TGTGCCCAAG 
AACATCGCCT TCTACCCCAG CAACCATGAG CGCTACCTGG AGGGCCACAT CGATGTGTGG 
TGGATTGTGC ATGATGGTGG CATGCTTATG CTTTTGCCCT TCCTGCTGCG CCAGCACAAG 
GTTTGGAGGA AGTGCCGGAT GCGCATCTTC ACTGTGGCCC AGATGGATGA CAACAGCATC 
CAGATGAAGA AGGATCTGGC CATCTTCCTG TACCATCTAC GCCTTGAGGC AGAGGTAGAG 
GTAGTAGAGA TGCACAACAG CGACATCTCT GCATATACCT ATGAGCGGAC ACTGATGATG 
GAGCAGCGGT CCCAGATGCT GCGGCAGATG AGACTGACCA AGACTGAGCG GGAGCGAGAA 
GCCCAGCTGG TCAAGGACCG GCATTCAGCC TTGCGACTGG AAAGCCTGTA CTCAGACGAG 
GAAGATGAGT CTGCAGCTGG GGCTGACAAG ATCCAGATGA CATGGACACG GGACAAGTAT 
ATGGCAGCTG AACCCTGGGA CCCCAGCCAT GCCCCTGACA ACTTCCGAGA ACTGGTCCAT 
ATTAAGCCGG ACCAGTCCAA TGTGCGGCGC ATGCACACTG CTGTGAAGCT CAATGAAGTC 
ATTGTCACAC GCTCCCATGA TGCCCGATTG GTCCTACTGA ACATGCCTGG CCCACCCAAG 
AACAGCGAGG GCGATGAGAA CTACATGGAG TTCCTGGAGG TACTGACCGA GGGCCTTGAG 
CGGGTGCTGT TGGTGCGTGG CGGTGGCCGT GAAGTCATCA CCATCTACTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A4 ( XM_025045252.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_024901020.1 Pteropus alecto solute carrier family 12 member 4 (SLC12A4), mRNA.

Price & Availability↑

CloneID	OPt85549
Clone ID Related Accession (Same CDS sequence)	XM_025045252.1
Accession Version	XM_025045252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	solute carrier family 12 member 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006434825.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCACT TCACTGTGGT ACCGGTGGAC GGGCCGCGGC GCGGCGACTA TGACAACCTC 
GAGGGGCTCA GTTGGGTGGA CTACGGGGAG CGCGCCGAGC GAGAAGACTC AGACGGACAC 
GGCAACCACA AAGAAAGCAG CCCTTTCCTT TGTCCCTTGG AGGCTTCCAG AGGAAGTGAA 
TACTATGACA GGAACCTGGC ACTGTTTGAG GAAGAGCTGG ACATCCGCCC AAAGGTGTCA 
TCTCTTCTGG GCAAACTCGT CAGCTACACC AATCTCACAC AAGGCGCCAA GGAGCATGAG 
GAGGCTGAAA GTGGGGAGGG CACCCGCCGG AGAGCAGCCA AGGCACCTAG CATGGGCACC 
CTCATGGGGG TGTACCTACC CTGCCTGCAG AATATTTTTG GAGTTATCCT CTTCCTGCGG 
CTGACCTGGA TGGTGGGAAC AGCTGGTGTG CTACAAGCCC TCCTCATTGT TCTCATCTGC 
TGCTGCTGTA CCTTGCTGAC GGCCATCTCC ATGAGCGCCA TCGCCACCAA TGGAGTGGTT 
CCAGCCGGGG GCTCCTATTT CATGATCTCC CGCTCGCTGG GGCCAGAATT TGGAGGTGCT 
GTGGGCCTAT GCTTCTACCT GGGAACAACA TTTGCGGCGG CCATGTACAT CCTGGGGGCC 
ATTGAGATCT TGCTGACTTA CATTGCCCCG CCAGCTGCCA TTTTTTACCC AACAGGCGCT 
CATGATACAT CAAGTGCCAC CTTGAACAAC ATGCGAGTGT ATGGGACCAT TTTTCTAACC 
TTCATGACCC TGGTGGTGTT TGTTGGTGTC AAGTATGTGA ACAAATTTGC CTCACTCTTC 
CTGGCCTGTG TGATCATCTC CATCCTCTCC ATCTATGCTG GGGGCATCAA GTCTATTTTT 
GATCCGCCCA TATTTCCAGT CTGCATGCTG GGCAACAGGA CCCTATCACG GGACCAGTTT 
GACATCTGTG CCAAGACGGC CATAGTGGAT AATGAGACAG TGGCCACACA GCTATGGAGC 
CTCTTCTGCC ACAGCACCAA CCTTACCACT GACTCCTGCG ACTCTTACTT CCTGCTCAAC 
AATGTGACCG AGATCCCTGG CATTCCAGGT GCAGCTGCTG GCGTGCTCCA GGAGAACCTG 
TGGAGCACCT ACCTGGAGAA GGGTGAGGTC GTGGAGAAGC ACGGGCTGCC TTCCACAGAC 
GCCCTTGGCG TGAAGGAGAG CCTGCCCCTA TACGTGGTGG CTGACATTGC CACATCCTTC 
ACTGTGCTGG TCGGCATCTT CTTCCCCTCT GTGACAGGCA TTATGGCTGG CTCAAATCGC 
TCCGGGGACC TCCGCGACGC CCAGAAGTCC ATTCCAGTGG GGACCATTCT GGCCATTGTT 
ACAACTTCCC TTGTGTACTT CAGCAGTGTG GTTCTCTTTG GCGCCTGCAT TGAGGGTGTG 
GTACTCCGGG ACAAGCTAGT GGTGGGCACA TTGGCCTGGC CTTCACCCTG GGTCATCGTC 
ATCGGCTCCT TCTTCTCAAC TTGTGGTGCC GGCCTACAGA GCCTCACTGG GGCACCACGC 
CTGTTGCAGG CCATTGCCAA GGACAACATC ATCCCCTTCC TCCGGGTATT TGGCCACGGG 
AAGGCAAATG GTGAACCAAC ATGGGCACTC CTCCTGACGG CACTCATCGC TGAGCTGGGC 
ATCCTCATTG CCTCCCTCGA CATGGTGGCC CCCATTCTGT CCATGTTCTT TCTGATGTGT 
TACCTGTTTG TGAACCTGGC CTGTGCTGTG CAGACGCTCC TGAGGACCCC CAACTGGCGG 
CCCCGGTTCA AGTACTATCA CTGGGCACTG TCCTTCCTGG GCATGAGTCT CTGCCTGGCC 
CTTATGTTTG TCTCCTCCTG GTACTATGCC CTGGTTGCCA TGCTCATTGC GGGCATGATC 
TACAAGTACA TTGAGTACCA AGGGGCTGAG AAGGAATGGG GTGACGGGAT CCGAGGCCTG 
TCCCTGAGTG CTGCCCGTTA CGCATTGTTG AGATTAGAGG AGGGGCCTCC TCACACCAAG 
AACTGGCGGC CTCAGCTGCT GGTGCTGCTG AAGCTAGATG AGGATCTTCA CGTGAAGTAC 
CCACGGCTCC TCACCTTTGC GTCCCAGCTG AAGGCAGGCA AGGGCTTGAC CATCGTTGGT 
TCTGTCATCC AGGGAAGCTT CTTGGAGAGC TATGGCGAGG CCCAGGCCGC TGAGCAGACA 
ATCAAGAACA TGATGGAGAT TGAGAAAGTG AAGGGCTTCT GCCAGGTGGT GGTAGCCAGC 
AAGGTGCGTG AAGGGCTGGC CCACCTCATC CAGTCTTGCG GCCTAGGCGG CATGAGGCAT 
AATTCCGTGG TGCTGGGCTG GCCCTATGGC TGGCGACAGA GTGAGGACCC ACGTGCCTGG 
AAGACCTTTA TTGACACCGT TCGTTGCACC ACGGCTGCCC ACCTGGCCCT GCTTGTGCCC 
AAGAACATCG CCTTCTACCC CAGCAACCAT GAGCGCTACC TGGAGGGCCA CATCGATGTG 
TGGTGGATTG TGCATGATGG TGGCATGCTT ATGCTTTTGC CCTTCCTGCT GCGCCAGCAC 
AAGGTTTGGA GGAAGTGCCG GATGCGCATC TTCACTGTGG CCCAGATGGA TGACAACAGC 
ATCCAGATGA AGAAGGATCT GGCCATCTTC CTGTACCATC TACGCCTTGA GGCAGAGGTA 
GAGGTAGTAG AGATGCACAA CAGCGACATC TCTGCATATA CCTATGAGCG GACACTGATG 
ATGGAGCAGC GGTCCCAGAT GCTGCGGCAG ATGAGACTGA CCAAGACTGA GCGGGAGCGA 
GAAGCCCAGC TGGTCAAGGA CCGGCATTCA GCCTTGCGAC TGGAAAGCCT GTACTCAGAC 
GAGGAAGATG AGTCTGCAGC TGGGGCTGAC AAGATCCAGA TGACATGGAC ACGGGACAAG 
TATATGGCAG CTGAACCCTG GGACCCCAGC CATGCCCCTG ACAACTTCCG AGAACTGGTC 
CATATTAAGC CGGACCAGTC CAATGTGCGG CGCATGCACA CTGCTGTGAA GCTCAATGAA 
GTCATTGTCA CACGCTCCCA TGATGCCCGA TTGGTCCTAC TGAACATGCC TGGCCCACCC 
AAGAACAGCG AGGGCGATGA GAACTACATG GAGTTCCTGG AGGTACTGAC CGAGGGCCTT 
GAGCGGGTGC TGTTGGTGCG TGGCGGTGGC CGTGAAGTCA TCACCATCTA CTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024901020.1
CDS	85..3321
Translation	MPHFTVVPVDGPRRGDYDNLEGLSWVDYGERAEREDSDGHGNHKESSPFLCPLEASRGSEYYDRNLALFEEELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGTRRRAAKAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPTGAHDTSSATLNNMRVYGTIFLTFMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPPIFPVCMLGNRTLSRDQFDICAKTAIVDNETVATQLWSLFCHSTNLTTDSCDSYFLLNNVTEIPGIPGAAAGVLQENLWSTYLEKGEVVEKHGLPSTDALGVKESLPLYVVADIATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVVLFGACIEGVVLRDKLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMEIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFYPSNHERYLEGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTEREREAQLVKDRHSALRLESLYSDEEDESAAGADKIQMTWTRDKYMAAEPWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPKNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_025045252.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto solute carrier family 12 member 4 (SLC12A4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025045252.1

ORF Insert Sequence:

ATGCCTCACT TCACTGTGGT ACCGGTGGAC GGGCCGCGGC GCGGCGACTA TGACAACCTC 
GAGGGGCTCA GTTGGGTGGA CTACGGGGAG CGCGCCGAGC GAGAAGACTC AGACGGACAC 
GGCAACCACA AAGAAAGCAG CCCTTTCCTT TGTCCCTTGG AGGCTTCCAG AGGAAGTGAA 
TACTATGACA GGAACCTGGC ACTGTTTGAG GAAGAGCTGG ACATCCGCCC AAAGGTGTCA 
TCTCTTCTGG GCAAACTCGT CAGCTACACC AATCTCACAC AAGGCGCCAA GGAGCATGAG 
GAGGCTGAAA GTGGGGAGGG CACCCGCCGG AGAGCAGCCA AGGCACCTAG CATGGGCACC 
CTCATGGGGG TGTACCTACC CTGCCTGCAG AATATTTTTG GAGTTATCCT CTTCCTGCGG 
CTGACCTGGA TGGTGGGAAC AGCTGGTGTG CTACAAGCCC TCCTCATTGT TCTCATCTGC 
TGCTGCTGTA CCTTGCTGAC GGCCATCTCC ATGAGCGCCA TCGCCACCAA TGGAGTGGTT 
CCAGCCGGGG GCTCCTATTT CATGATCTCC CGCTCGCTGG GGCCAGAATT TGGAGGTGCT 
GTGGGCCTAT GCTTCTACCT GGGAACAACA TTTGCGGCGG CCATGTACAT CCTGGGGGCC 
ATTGAGATCT TGCTGACTTA CATTGCCCCG CCAGCTGCCA TTTTTTACCC AACAGGCGCT 
CATGATACAT CAAGTGCCAC CTTGAACAAC ATGCGAGTGT ATGGGACCAT TTTTCTAACC 
TTCATGACCC TGGTGGTGTT TGTTGGTGTC AAGTATGTGA ACAAATTTGC CTCACTCTTC 
CTGGCCTGTG TGATCATCTC CATCCTCTCC ATCTATGCTG GGGGCATCAA GTCTATTTTT 
GATCCGCCCA TATTTCCAGT CTGCATGCTG GGCAACAGGA CCCTATCACG GGACCAGTTT 
GACATCTGTG CCAAGACGGC CATAGTGGAT AATGAGACAG TGGCCACACA GCTATGGAGC 
CTCTTCTGCC ACAGCACCAA CCTTACCACT GACTCCTGCG ACTCTTACTT CCTGCTCAAC 
AATGTGACCG AGATCCCTGG CATTCCAGGT GCAGCTGCTG GCGTGCTCCA GGAGAACCTG 
TGGAGCACCT ACCTGGAGAA GGGTGAGGTC GTGGAGAAGC ACGGGCTGCC TTCCACAGAC 
GCCCTTGGCG TGAAGGAGAG CCTGCCCCTA TACGTGGTGG CTGACATTGC CACATCCTTC 
ACTGTGCTGG TCGGCATCTT CTTCCCCTCT GTGACAGGCA TTATGGCTGG CTCAAATCGC 
TCCGGGGACC TCCGCGACGC CCAGAAGTCC ATTCCAGTGG GGACCATTCT GGCCATTGTT 
ACAACTTCCC TTGTGTACTT CAGCAGTGTG GTTCTCTTTG GCGCCTGCAT TGAGGGTGTG 
GTACTCCGGG ACAAGCTAGT GGTGGGCACA TTGGCCTGGC CTTCACCCTG GGTCATCGTC 
ATCGGCTCCT TCTTCTCAAC TTGTGGTGCC GGCCTACAGA GCCTCACTGG GGCACCACGC 
CTGTTGCAGG CCATTGCCAA GGACAACATC ATCCCCTTCC TCCGGGTATT TGGCCACGGG 
AAGGCAAATG GTGAACCAAC ATGGGCACTC CTCCTGACGG CACTCATCGC TGAGCTGGGC 
ATCCTCATTG CCTCCCTCGA CATGGTGGCC CCCATTCTGT CCATGTTCTT TCTGATGTGT 
TACCTGTTTG TGAACCTGGC CTGTGCTGTG CAGACGCTCC TGAGGACCCC CAACTGGCGG 
CCCCGGTTCA AGTACTATCA CTGGGCACTG TCCTTCCTGG GCATGAGTCT CTGCCTGGCC 
CTTATGTTTG TCTCCTCCTG GTACTATGCC CTGGTTGCCA TGCTCATTGC GGGCATGATC 
TACAAGTACA TTGAGTACCA AGGGGCTGAG AAGGAATGGG GTGACGGGAT CCGAGGCCTG 
TCCCTGAGTG CTGCCCGTTA CGCATTGTTG AGATTAGAGG AGGGGCCTCC TCACACCAAG 
AACTGGCGGC CTCAGCTGCT GGTGCTGCTG AAGCTAGATG AGGATCTTCA CGTGAAGTAC 
CCACGGCTCC TCACCTTTGC GTCCCAGCTG AAGGCAGGCA AGGGCTTGAC CATCGTTGGT 
TCTGTCATCC AGGGAAGCTT CTTGGAGAGC TATGGCGAGG CCCAGGCCGC TGAGCAGACA 
ATCAAGAACA TGATGGAGAT TGAGAAAGTG AAGGGCTTCT GCCAGGTGGT GGTAGCCAGC 
AAGGTGCGTG AAGGGCTGGC CCACCTCATC CAGTCTTGCG GCCTAGGCGG CATGAGGCAT 
AATTCCGTGG TGCTGGGCTG GCCCTATGGC TGGCGACAGA GTGAGGACCC ACGTGCCTGG 
AAGACCTTTA TTGACACCGT TCGTTGCACC ACGGCTGCCC ACCTGGCCCT GCTTGTGCCC 
AAGAACATCG CCTTCTACCC CAGCAACCAT GAGCGCTACC TGGAGGGCCA CATCGATGTG 
TGGTGGATTG TGCATGATGG TGGCATGCTT ATGCTTTTGC CCTTCCTGCT GCGCCAGCAC 
AAGGTTTGGA GGAAGTGCCG GATGCGCATC TTCACTGTGG CCCAGATGGA TGACAACAGC 
ATCCAGATGA AGAAGGATCT GGCCATCTTC CTGTACCATC TACGCCTTGA GGCAGAGGTA 
GAGGTAGTAG AGATGCACAA CAGCGACATC TCTGCATATA CCTATGAGCG GACACTGATG 
ATGGAGCAGC GGTCCCAGAT GCTGCGGCAG ATGAGACTGA CCAAGACTGA GCGGGAGCGA 
GAAGCCCAGC TGGTCAAGGA CCGGCATTCA GCCTTGCGAC TGGAAAGCCT GTACTCAGAC 
GAGGAAGATG AGTCTGCAGC TGGGGCTGAC AAGATCCAGA TGACATGGAC ACGGGACAAG 
TATATGGCAG CTGAACCCTG GGACCCCAGC CATGCCCCTG ACAACTTCCG AGAACTGGTC 
CATATTAAGC CGGACCAGTC CAATGTGCGG CGCATGCACA CTGCTGTGAA GCTCAATGAA 
GTCATTGTCA CACGCTCCCA TGATGCCCGA TTGGTCCTAC TGAACATGCC TGGCCCACCC 
AAGAACAGCG AGGGCGATGA GAACTACATG GAGTTCCTGG AGGTACTGAC CGAGGGCCTT 
GAGCGGGTGC TGTTGGTGCG TGGCGGTGGC CGTGAAGTCA TCACCATCTA CTCTTGA