RNF216 cDNA ORF clone, Pogona vitticeps(central bearded dragon)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF216
Entrez Gene ID	110083722
Full Name	ring finger protein 216
Gene Type	protein-coding
Organism	Pogona vitticeps(central bearded dragon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020802401.1	XP_020658060.1	E3 ubiquitin-protein ligase RNF216 isoform X1
XM_020802403.1	XP_020658062.1	E3 ubiquitin-protein ligase RNF216 isoform X2

Pathways from BioSystems
Gene Ontology

The following RNF216 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF216 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo249224	XM_020802401.1 Latest version!	Pogona vitticeps ring finger protein 216 (RNF216), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPo249225	XM_020802403.1 Latest version!	Pogona vitticeps ring finger protein 216 (RNF216), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF216 ( XM_020802401.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020658060.1 Pogona vitticeps ring finger protein 216 (RNF216), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo249224
Clone ID Related Accession (Same CDS sequence)	XM_020802401.1
Accession Version	XM_020802401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	E3 ubiquitin-protein ligase RNF216 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018151017.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG GAGGTGGAAA AGAAGAGGTG ATTGATCTGA ATAGTTTCCG GAGCCACCGA 
GGGAAAGACA GAATTAACCA CAGAGACGAG GGGCTTATCA CCATATCGGA CTCCTCGGAT 
GAAGACTTGC CTGTGATGCT GGACATACCT GTTCACCGGC AGTGGGAAGA TGAGGAAGAT 
GATGATGTAG TCATCTTGAT GGAGCCTGCC TCGAAGAAGC TGCTGCGCCC CAACGTGATC 
CGGCCGGCTG CCCCCTGGCA AAGCGCCAGC AAGTCAGGGG AGGGGGGATC CCAAGGTGAC 
GTGGCAGCCA CCGGGCCCCC CGAGAGGCAG AAGGCGAGGA CCCCTCCCTC TCCGAGGCGA 
CCCGGCGCTC GCCGGAACGG CCCGAAGGCG AGCGGCGTGG TCCGGGGCAG GAACGAAAGG 
GAGGAAGAGG TTTTGGAGCT GCCCGACTCC CCCGAGCCGA GAGAGCTTGG TGGAACTGTT 
TCGGAGCCTG GCCCCAGCGC GGGTCGGACG CCAGAGGCAA GGCCCGGCGG CGAAAAGGAA 
GTGATTAATC TGGAGGAGGT GGTCCTGGTG GCTGATCCGG AGGAGCCACC CCGGAGGGAT 
CGGGAACTGC AGCCGCAGCT AGTGCCAAAC CTCGGCGGCT CCGTCCAGTG CGTGGAGGAG 
GACGCTCATC GAGAGCACCC TTACTTCCAG CCGACAGAGG AGGAGATCCG TGTGGTGCTT 
GACCTCCTTG CTCCTAGGCA GGACCTTGGG CCGCTGCCGA GGGTGTATCA AGAGGAGATC 
CCGGGCCCTG CCTTTCCCAG GCCCGAACCA CAGCAGGATG GAATCCCAGG GCCGGCTTCC 
CCTCAGCTGG CTCACCCACC GGAGGAAAAG GGAGGCCCGC AAGCAGCAAT AAACGAACAG 
GCTGGCCCGG CTTTTCCAGT CCAGGGCTCT CTAGATTCCG GCTCCAGCGA GATTCCCAAA 
CAAGGTGCTG TTCAGGTGGA CAAAGACTTC ATTGCTCTGC TCGTCAGAGA AACAGAAGCG 
AGGTTTCCAG ATGCAACAAG AGAGTATATT GAGGAACTTA TACAGAGCAA AAACTGCTGT 
GACTTAAACA TACTCTGTAA TTTTCTTCTG GAAAACCCAG ATTATCCAAA GAAGGAAGGC 
AGAGTGGTGT TAAATCCCAA TAGCAGCTTG CTGTCCAGCC AAGATGAGAC AAAGGTGCCT 
AAAGTAGACT ACTTTGATCT CTCCAAACTG GACCCACTTG ATCAACGTTG TTTCATCCAA 
GCGGCCGACC TCCTCATGGC AGACTTCAAG ATGCTGAGCA GCCAGGACAT CAAATGGGCC 
CTCCACGAAC TCAAAGGACA CTATGCAATC ACACGAAAGG CCTTCTCCGA TGCCATCAAA 
AAATGGCAGG AGCTGTCTCC TGAAACCAGT GGCAAGCGAA AACGAAGGAA AGAGATGAAC 
CAGTATTCTT ACATAGATTT CAAATTCGAA CAAGGTGATA TGAAAATTGA AAAGCGCATG 
TTCTTCTTGG AGAACAAGCG GCGACACTGC AGGAGCTATG ATCCCCTCTC TCTCCTCCCA 
GCCGTTCAGC TGGAACGGGA GTTTTACGAA CAGAAGATTA AAGAAATGGC CGAGCATGCA 
GACTTCCTCC TTGCCCTGCA GATGAATGAA GAACAGTATC AAAAAGACGG TCAGATGATC 
GAATGCCGCT GTTGCTACGG GGAGTTTGCG TTCGAAGAAC TCACGCAGTG TGCCGACGGA 
CACTTGTTTT GCAAAGAGTG CCTAATAAAG TATGCTCAGG AGGCTGTCTT TGGCTCCGGA 
AAGTCGGAGC TCAGCTGCAT GGAAGGGAGC TGCACCTGCT CGTTTCCGAC GAGCGAGCTG 
GAGAAAGTCC TTCCTGAAAA CATCCTATGT AAATACTACG AGCGGAAAGC AGAGGAAGAG 
GTGGCTGCGG CCTGCGCGGA TGAGCTAGTC AGGTGTCCCT TCTGCAACTT TCCAGCGTTA 
CTGGACAAGG AAGTGAAGCG GTTCAGCTGT CCTAACCCTC GGTGCAGGAA GGAAACTTGC 
AGAAAATGCC AGGGCCTTTG GAAGGAGCAC ATGAACCTTA CCTGTGAACA GTTGGCTGAG 
AAGGATGACA TAAAATACAG AACCTCTATA GAAGAGAAGA TGACGGCGGC CCGGATTAGG 
AAATGCCACA AGTGCGCGAC CGGCTTGATC AAATCAGAAG GCTGCAACCG TATGTCGTGC 
CGCTGCGGGG CCCAGATGTG CTACCTCTGC CGAGCCGCCA TAAACGGGTA CGACCACTTC 
TGCCAGCACC CCCGATCCCC GGGAGCCCCT TGCCAAGACT GCGCAAAATG TTCCCTTTGG 
ACAGACCCCA CGGAGGACGA TGAGAAGATA ATCCAGGAGA TTCAGAAGGA AGCTGAAGAG 
GAGCAAAAGA GGAAGAACGG AGAAAACAGT TTCAAGCGGA TTGGGCCACC AGTAGAAAAG 
CCGGCAGAGA AAATCCCACG GATTGAAGCC ATTCCCAGGC CGGTTCCCCA GAACCTTCAC 
CACCAAATAC CCCCTTACCC CTTTGTGCAC CCTCCCTTCC CCTTACCCCC CGTCCGCCCG 
CTGTACAATA ACATCGGCCC CATCAACCTG GGGCCCATCC CTGCCCCTTA CGTGCCCCCA 
CTGCCTAACA TGCGGGTGAA CTACGAGTTT CCCCAGATGA ACCTGCAGCT GGAGCACAAT 
CTGCCTATGC ACTTTGGTCC ACAGCCTCGG CATCGGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020658060.1
CDS	65..2806
Translation	MEEGGGKEEVIDLNSFRSHRGKDRINHRDEGLITISDSSDEDLPVMLDIPVHRQWEDEEDDDVVILMEPASKKLLRPNVIRPAAPWQSASKSGEGGSQGDVAATGPPERQKARTPPSPRRPGARRNGPKASGVVRGRNEREEEVLELPDSPEPRELGGTVSEPGPSAGRTPEARPGGEKEVINLEEVVLVADPEEPPRRDRELQPQLVPNLGGSVQCVEEDAHREHPYFQPTEEEIRVVLDLLAPRQDLGPLPRVYQEEIPGPAFPRPEPQQDGIPGPASPQLAHPPEEKGGPQAAINEQAGPAFPVQGSLDSGSSEIPKQGAVQVDKDFIALLVRETEARFPDATREYIEELIQSKNCCDLNILCNFLLENPDYPKKEGRVVLNPNSSLLSSQDETKVPKVDYFDLSKLDPLDQRCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKRRKEMNQYSYIDFKFEQGDMKIEKRMFFLENKRRHCRSYDPLSLLPAVQLEREFYEQKIKEMAEHADFLLALQMNEEQYQKDGQMIECRCCYGEFAFEELTQCADGHLFCKECLIKYAQEAVFGSGKSELSCMEGSCTCSFPTSELEKVLPENILCKYYERKAEEEVAAACADELVRCPFCNFPALLDKEVKRFSCPNPRCRKETCRKCQGLWKEHMNLTCEQLAEKDDIKYRTSIEEKMTAARIRKCHKCATGLIKSEGCNRMSCRCGAQMCYLCRAAINGYDHFCQHPRSPGAPCQDCAKCSLWTDPTEDDEKIIQEIQKEAEEEQKRKNGENSFKRIGPPVEKPAEKIPRIEAIPRPVPQNLHHQIPPYPFVHPPFPLPPVRPLYNNIGPINLGPIPAPYVPPLPNMRVNYEFPQMNLQLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_020802401.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps ring finger protein 216 (RNF216), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020802401.1

ORF Insert Sequence:

ATGGAAGAAG GAGGTGGAAA AGAAGAGGTG ATTGATCTGA ATAGTTTCCG GAGCCACCGA 
GGGAAAGACA GAATTAACCA CAGAGACGAG GGGCTTATCA CCATATCGGA CTCCTCGGAT 
GAAGACTTGC CTGTGATGCT GGACATACCT GTTCACCGGC AGTGGGAAGA TGAGGAAGAT 
GATGATGTAG TCATCTTGAT GGAGCCTGCC TCGAAGAAGC TGCTGCGCCC CAACGTGATC 
CGGCCGGCTG CCCCCTGGCA AAGCGCCAGC AAGTCAGGGG AGGGGGGATC CCAAGGTGAC 
GTGGCAGCCA CCGGGCCCCC CGAGAGGCAG AAGGCGAGGA CCCCTCCCTC TCCGAGGCGA 
CCCGGCGCTC GCCGGAACGG CCCGAAGGCG AGCGGCGTGG TCCGGGGCAG GAACGAAAGG 
GAGGAAGAGG TTTTGGAGCT GCCCGACTCC CCCGAGCCGA GAGAGCTTGG TGGAACTGTT 
TCGGAGCCTG GCCCCAGCGC GGGTCGGACG CCAGAGGCAA GGCCCGGCGG CGAAAAGGAA 
GTGATTAATC TGGAGGAGGT GGTCCTGGTG GCTGATCCGG AGGAGCCACC CCGGAGGGAT 
CGGGAACTGC AGCCGCAGCT AGTGCCAAAC CTCGGCGGCT CCGTCCAGTG CGTGGAGGAG 
GACGCTCATC GAGAGCACCC TTACTTCCAG CCGACAGAGG AGGAGATCCG TGTGGTGCTT 
GACCTCCTTG CTCCTAGGCA GGACCTTGGG CCGCTGCCGA GGGTGTATCA AGAGGAGATC 
CCGGGCCCTG CCTTTCCCAG GCCCGAACCA CAGCAGGATG GAATCCCAGG GCCGGCTTCC 
CCTCAGCTGG CTCACCCACC GGAGGAAAAG GGAGGCCCGC AAGCAGCAAT AAACGAACAG 
GCTGGCCCGG CTTTTCCAGT CCAGGGCTCT CTAGATTCCG GCTCCAGCGA GATTCCCAAA 
CAAGGTGCTG TTCAGGTGGA CAAAGACTTC ATTGCTCTGC TCGTCAGAGA AACAGAAGCG 
AGGTTTCCAG ATGCAACAAG AGAGTATATT GAGGAACTTA TACAGAGCAA AAACTGCTGT 
GACTTAAACA TACTCTGTAA TTTTCTTCTG GAAAACCCAG ATTATCCAAA GAAGGAAGGC 
AGAGTGGTGT TAAATCCCAA TAGCAGCTTG CTGTCCAGCC AAGATGAGAC AAAGGTGCCT 
AAAGTAGACT ACTTTGATCT CTCCAAACTG GACCCACTTG ATCAACGTTG TTTCATCCAA 
GCGGCCGACC TCCTCATGGC AGACTTCAAG ATGCTGAGCA GCCAGGACAT CAAATGGGCC 
CTCCACGAAC TCAAAGGACA CTATGCAATC ACACGAAAGG CCTTCTCCGA TGCCATCAAA 
AAATGGCAGG AGCTGTCTCC TGAAACCAGT GGCAAGCGAA AACGAAGGAA AGAGATGAAC 
CAGTATTCTT ACATAGATTT CAAATTCGAA CAAGGTGATA TGAAAATTGA AAAGCGCATG 
TTCTTCTTGG AGAACAAGCG GCGACACTGC AGGAGCTATG ATCCCCTCTC TCTCCTCCCA 
GCCGTTCAGC TGGAACGGGA GTTTTACGAA CAGAAGATTA AAGAAATGGC CGAGCATGCA 
GACTTCCTCC TTGCCCTGCA GATGAATGAA GAACAGTATC AAAAAGACGG TCAGATGATC 
GAATGCCGCT GTTGCTACGG GGAGTTTGCG TTCGAAGAAC TCACGCAGTG TGCCGACGGA 
CACTTGTTTT GCAAAGAGTG CCTAATAAAG TATGCTCAGG AGGCTGTCTT TGGCTCCGGA 
AAGTCGGAGC TCAGCTGCAT GGAAGGGAGC TGCACCTGCT CGTTTCCGAC GAGCGAGCTG 
GAGAAAGTCC TTCCTGAAAA CATCCTATGT AAATACTACG AGCGGAAAGC AGAGGAAGAG 
GTGGCTGCGG CCTGCGCGGA TGAGCTAGTC AGGTGTCCCT TCTGCAACTT TCCAGCGTTA 
CTGGACAAGG AAGTGAAGCG GTTCAGCTGT CCTAACCCTC GGTGCAGGAA GGAAACTTGC 
AGAAAATGCC AGGGCCTTTG GAAGGAGCAC ATGAACCTTA CCTGTGAACA GTTGGCTGAG 
AAGGATGACA TAAAATACAG AACCTCTATA GAAGAGAAGA TGACGGCGGC CCGGATTAGG 
AAATGCCACA AGTGCGCGAC CGGCTTGATC AAATCAGAAG GCTGCAACCG TATGTCGTGC 
CGCTGCGGGG CCCAGATGTG CTACCTCTGC CGAGCCGCCA TAAACGGGTA CGACCACTTC 
TGCCAGCACC CCCGATCCCC GGGAGCCCCT TGCCAAGACT GCGCAAAATG TTCCCTTTGG 
ACAGACCCCA CGGAGGACGA TGAGAAGATA ATCCAGGAGA TTCAGAAGGA AGCTGAAGAG 
GAGCAAAAGA GGAAGAACGG AGAAAACAGT TTCAAGCGGA TTGGGCCACC AGTAGAAAAG 
CCGGCAGAGA AAATCCCACG GATTGAAGCC ATTCCCAGGC CGGTTCCCCA GAACCTTCAC 
CACCAAATAC CCCCTTACCC CTTTGTGCAC CCTCCCTTCC CCTTACCCCC CGTCCGCCCG 
CTGTACAATA ACATCGGCCC CATCAACCTG GGGCCCATCC CTGCCCCTTA CGTGCCCCCA 
CTGCCTAACA TGCGGGTGAA CTACGAGTTT CCCCAGATGA ACCTGCAGCT GGAGCACAAT 
CTGCCTATGC ACTTTGGTCC ACAGCCTCGG CATCGGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_020802403.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020658062.1 Pogona vitticeps ring finger protein 216 (RNF216), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo249225
Clone ID Related Accession (Same CDS sequence)	XM_020802403.1
Accession Version	XM_020802403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	E3 ubiquitin-protein ligase RNF216 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018151017.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGACA TACCTGTTCA CCGGCAGTGG GAAGATGAGG AAGATGATGA TGTAGTCATC 
TTGATGGAGC CTGCCTCGAA GAAGCTGCTG CGCCCCAACG TGATCCGGCC GGCTGCCCCC 
TGGCAAAGCG CCAGCAAGTC AGGGGAGGGG GGATCCCAAG GTGACGTGGC AGCCACCGGG 
CCCCCCGAGA GGCAGAAGGC GAGGACCCCT CCCTCTCCGA GGCGACCCGG CGCTCGCCGG 
AACGGCCCGA AGGCGAGCGG CGTGGTCCGG GGCAGGAACG AAAGGGAGGA AGAGGTTTTG 
GAGCTGCCCG ACTCCCCCGA GCCGAGAGAG CTTGGTGGAA CTGTTTCGGA GCCTGGCCCC 
AGCGCGGGTC GGACGCCAGA GGCAAGGCCC GGCGGCGAAA AGGAAGTGAT TAATCTGGAG 
GAGGTGGTCC TGGTGGCTGA TCCGGAGGAG CCACCCCGGA GGGATCGGGA ACTGCAGCCG 
CAGCTAGTGC CAAACCTCGG CGGCTCCGTC CAGTGCGTGG AGGAGGACGC TCATCGAGAG 
CACCCTTACT TCCAGCCGAC AGAGGAGGAG ATCCGTGTGG TGCTTGACCT CCTTGCTCCT 
AGGCAGGACC TTGGGCCGCT GCCGAGGGTG TATCAAGAGG AGATCCCGGG CCCTGCCTTT 
CCCAGGCCCG AACCACAGCA GGATGGAATC CCAGGGCCGG CTTCCCCTCA GCTGGCTCAC 
CCACCGGAGG AAAAGGGAGG CCCGCAAGCA GCAATAAACG AACAGGCTGG CCCGGCTTTT 
CCAGTCCAGG GCTCTCTAGA TTCCGGCTCC AGCGAGATTC CCAAACAAGG TGCTGTTCAG 
GTGGACAAAG ACTTCATTGC TCTGCTCGTC AGAGAAACAG AAGCGAGGTT TCCAGATGCA 
ACAAGAGAGT ATATTGAGGA ACTTATACAG AGCAAAAACT GCTGTGACTT AAACATACTC 
TGTAATTTTC TTCTGGAAAA CCCAGATTAT CCAAAGAAGG AAGGCAGAGT GGTGTTAAAT 
CCCAATAGCA GCTTGCTGTC CAGCCAAGAT GAGACAAAGG TGCCTAAAGT AGACTACTTT 
GATCTCTCCA AACTGGACCC ACTTGATCAA CGTTGTTTCA TCCAAGCGGC CGACCTCCTC 
ATGGCAGACT TCAAGATGCT GAGCAGCCAG GACATCAAAT GGGCCCTCCA CGAACTCAAA 
GGACACTATG CAATCACACG AAAGGCCTTC TCCGATGCCA TCAAAAAATG GCAGGAGCTG 
TCTCCTGAAA CCAGTGGCAA GCGAAAACGA AGGAAAGAGA TGAACCAGTA TTCTTACATA 
GATTTCAAAT TCGAACAAGG TGATATGAAA ATTGAAAAGC GCATGTTCTT CTTGGAGAAC 
AAGCGGCGAC ACTGCAGGAG CTATGATCCC CTCTCTCTCC TCCCAGCCGT TCAGCTGGAA 
CGGGAGTTTT ACGAACAGAA GATTAAAGAA ATGGCCGAGC ATGCAGACTT CCTCCTTGCC 
CTGCAGATGA ATGAAGAACA GTATCAAAAA GACGGTCAGA TGATCGAATG CCGCTGTTGC 
TACGGGGAGT TTGCGTTCGA AGAACTCACG CAGTGTGCCG ACGGACACTT GTTTTGCAAA 
GAGTGCCTAA TAAAGTATGC TCAGGAGGCT GTCTTTGGCT CCGGAAAGTC GGAGCTCAGC 
TGCATGGAAG GGAGCTGCAC CTGCTCGTTT CCGACGAGCG AGCTGGAGAA AGTCCTTCCT 
GAAAACATCC TATGTAAATA CTACGAGCGG AAAGCAGAGG AAGAGGTGGC TGCGGCCTGC 
GCGGATGAGC TAGTCAGGTG TCCCTTCTGC AACTTTCCAG CGTTACTGGA CAAGGAAGTG 
AAGCGGTTCA GCTGTCCTAA CCCTCGGTGC AGGAAGGAAA CTTGCAGAAA ATGCCAGGGC 
CTTTGGAAGG AGCACATGAA CCTTACCTGT GAACAGTTGG CTGAGAAGGA TGACATAAAA 
TACAGAACCT CTATAGAAGA GAAGATGACG GCGGCCCGGA TTAGGAAATG CCACAAGTGC 
GCGACCGGCT TGATCAAATC AGAAGGCTGC AACCGTATGT CGTGCCGCTG CGGGGCCCAG 
ATGTGCTACC TCTGCCGAGC CGCCATAAAC GGGTACGACC ACTTCTGCCA GCACCCCCGA 
TCCCCGGGAG CCCCTTGCCA AGACTGCGCA AAATGTTCCC TTTGGACAGA CCCCACGGAG 
GACGATGAGA AGATAATCCA GGAGATTCAG AAGGAAGCTG AAGAGGAGCA AAAGAGGAAG 
AACGGAGAAA ACAGTTTCAA GCGGATTGGG CCACCAGTAG AAAAGCCGGC AGAGAAAATC 
CCACGGATTG AAGCCATTCC CAGGCCGGTT CCCCAGAACC TTCACCACCA AATACCCCCT 
TACCCCTTTG TGCACCCTCC CTTCCCCTTA CCCCCCGTCC GCCCGCTGTA CAATAACATC 
GGCCCCATCA ACCTGGGGCC CATCCCTGCC CCTTACGTGC CCCCACTGCC TAACATGCGG 
GTGAACTACG AGTTTCCCCA GATGAACCTG CAGCTGGAGC ACAATCTGCC TATGCACTTT 
GGTCCACAGC CTCGGCATCG GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020658062.1
CDS	153..2759
Translation	MLDIPVHRQWEDEEDDDVVILMEPASKKLLRPNVIRPAAPWQSASKSGEGGSQGDVAATGPPERQKARTPPSPRRPGARRNGPKASGVVRGRNEREEEVLELPDSPEPRELGGTVSEPGPSAGRTPEARPGGEKEVINLEEVVLVADPEEPPRRDRELQPQLVPNLGGSVQCVEEDAHREHPYFQPTEEEIRVVLDLLAPRQDLGPLPRVYQEEIPGPAFPRPEPQQDGIPGPASPQLAHPPEEKGGPQAAINEQAGPAFPVQGSLDSGSSEIPKQGAVQVDKDFIALLVRETEARFPDATREYIEELIQSKNCCDLNILCNFLLENPDYPKKEGRVVLNPNSSLLSSQDETKVPKVDYFDLSKLDPLDQRCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKRRKEMNQYSYIDFKFEQGDMKIEKRMFFLENKRRHCRSYDPLSLLPAVQLEREFYEQKIKEMAEHADFLLALQMNEEQYQKDGQMIECRCCYGEFAFEELTQCADGHLFCKECLIKYAQEAVFGSGKSELSCMEGSCTCSFPTSELEKVLPENILCKYYERKAEEEVAAACADELVRCPFCNFPALLDKEVKRFSCPNPRCRKETCRKCQGLWKEHMNLTCEQLAEKDDIKYRTSIEEKMTAARIRKCHKCATGLIKSEGCNRMSCRCGAQMCYLCRAAINGYDHFCQHPRSPGAPCQDCAKCSLWTDPTEDDEKIIQEIQKEAEEEQKRKNGENSFKRIGPPVEKPAEKIPRIEAIPRPVPQNLHHQIPPYPFVHPPFPLPPVRPLYNNIGPINLGPIPAPYVPPLPNMRVNYEFPQMNLQLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_020802403.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps ring finger protein 216 (RNF216), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020802403.1

ORF Insert Sequence:

ATGCTGGACA TACCTGTTCA CCGGCAGTGG GAAGATGAGG AAGATGATGA TGTAGTCATC 
TTGATGGAGC CTGCCTCGAA GAAGCTGCTG CGCCCCAACG TGATCCGGCC GGCTGCCCCC 
TGGCAAAGCG CCAGCAAGTC AGGGGAGGGG GGATCCCAAG GTGACGTGGC AGCCACCGGG 
CCCCCCGAGA GGCAGAAGGC GAGGACCCCT CCCTCTCCGA GGCGACCCGG CGCTCGCCGG 
AACGGCCCGA AGGCGAGCGG CGTGGTCCGG GGCAGGAACG AAAGGGAGGA AGAGGTTTTG 
GAGCTGCCCG ACTCCCCCGA GCCGAGAGAG CTTGGTGGAA CTGTTTCGGA GCCTGGCCCC 
AGCGCGGGTC GGACGCCAGA GGCAAGGCCC GGCGGCGAAA AGGAAGTGAT TAATCTGGAG 
GAGGTGGTCC TGGTGGCTGA TCCGGAGGAG CCACCCCGGA GGGATCGGGA ACTGCAGCCG 
CAGCTAGTGC CAAACCTCGG CGGCTCCGTC CAGTGCGTGG AGGAGGACGC TCATCGAGAG 
CACCCTTACT TCCAGCCGAC AGAGGAGGAG ATCCGTGTGG TGCTTGACCT CCTTGCTCCT 
AGGCAGGACC TTGGGCCGCT GCCGAGGGTG TATCAAGAGG AGATCCCGGG CCCTGCCTTT 
CCCAGGCCCG AACCACAGCA GGATGGAATC CCAGGGCCGG CTTCCCCTCA GCTGGCTCAC 
CCACCGGAGG AAAAGGGAGG CCCGCAAGCA GCAATAAACG AACAGGCTGG CCCGGCTTTT 
CCAGTCCAGG GCTCTCTAGA TTCCGGCTCC AGCGAGATTC CCAAACAAGG TGCTGTTCAG 
GTGGACAAAG ACTTCATTGC TCTGCTCGTC AGAGAAACAG AAGCGAGGTT TCCAGATGCA 
ACAAGAGAGT ATATTGAGGA ACTTATACAG AGCAAAAACT GCTGTGACTT AAACATACTC 
TGTAATTTTC TTCTGGAAAA CCCAGATTAT CCAAAGAAGG AAGGCAGAGT GGTGTTAAAT 
CCCAATAGCA GCTTGCTGTC CAGCCAAGAT GAGACAAAGG TGCCTAAAGT AGACTACTTT 
GATCTCTCCA AACTGGACCC ACTTGATCAA CGTTGTTTCA TCCAAGCGGC CGACCTCCTC 
ATGGCAGACT TCAAGATGCT GAGCAGCCAG GACATCAAAT GGGCCCTCCA CGAACTCAAA 
GGACACTATG CAATCACACG AAAGGCCTTC TCCGATGCCA TCAAAAAATG GCAGGAGCTG 
TCTCCTGAAA CCAGTGGCAA GCGAAAACGA AGGAAAGAGA TGAACCAGTA TTCTTACATA 
GATTTCAAAT TCGAACAAGG TGATATGAAA ATTGAAAAGC GCATGTTCTT CTTGGAGAAC 
AAGCGGCGAC ACTGCAGGAG CTATGATCCC CTCTCTCTCC TCCCAGCCGT TCAGCTGGAA 
CGGGAGTTTT ACGAACAGAA GATTAAAGAA ATGGCCGAGC ATGCAGACTT CCTCCTTGCC 
CTGCAGATGA ATGAAGAACA GTATCAAAAA GACGGTCAGA TGATCGAATG CCGCTGTTGC 
TACGGGGAGT TTGCGTTCGA AGAACTCACG CAGTGTGCCG ACGGACACTT GTTTTGCAAA 
GAGTGCCTAA TAAAGTATGC TCAGGAGGCT GTCTTTGGCT CCGGAAAGTC GGAGCTCAGC 
TGCATGGAAG GGAGCTGCAC CTGCTCGTTT CCGACGAGCG AGCTGGAGAA AGTCCTTCCT 
GAAAACATCC TATGTAAATA CTACGAGCGG AAAGCAGAGG AAGAGGTGGC TGCGGCCTGC 
GCGGATGAGC TAGTCAGGTG TCCCTTCTGC AACTTTCCAG CGTTACTGGA CAAGGAAGTG 
AAGCGGTTCA GCTGTCCTAA CCCTCGGTGC AGGAAGGAAA CTTGCAGAAA ATGCCAGGGC 
CTTTGGAAGG AGCACATGAA CCTTACCTGT GAACAGTTGG CTGAGAAGGA TGACATAAAA 
TACAGAACCT CTATAGAAGA GAAGATGACG GCGGCCCGGA TTAGGAAATG CCACAAGTGC 
GCGACCGGCT TGATCAAATC AGAAGGCTGC AACCGTATGT CGTGCCGCTG CGGGGCCCAG 
ATGTGCTACC TCTGCCGAGC CGCCATAAAC GGGTACGACC ACTTCTGCCA GCACCCCCGA 
TCCCCGGGAG CCCCTTGCCA AGACTGCGCA AAATGTTCCC TTTGGACAGA CCCCACGGAG 
GACGATGAGA AGATAATCCA GGAGATTCAG AAGGAAGCTG AAGAGGAGCA AAAGAGGAAG 
AACGGAGAAA ACAGTTTCAA GCGGATTGGG CCACCAGTAG AAAAGCCGGC AGAGAAAATC 
CCACGGATTG AAGCCATTCC CAGGCCGGTT CCCCAGAACC TTCACCACCA AATACCCCCT 
TACCCCTTTG TGCACCCTCC CTTCCCCTTA CCCCCCGTCC GCCCGCTGTA CAATAACATC 
GGCCCCATCA ACCTGGGGCC CATCCCTGCC CCTTACGTGC CCCCACTGCC TAACATGCGG 
GTGAACTACG AGTTTCCCCA GATGAACCTG CAGCTGGAGC ACAATCTGCC TATGCACTTT 
GGTCCACAGC CTCGGCATCG GTTCTGA