BRF2 cDNA ORF clone, Phascolarctos cinereus(koala)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRF2
Entrez Gene ID	110207595
Full Name	BRF2, RNA polymerase III transcription initiation factor subunit
Gene Type	protein-coding
Organism	Phascolarctos cinereus(koala)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020985279.1	XP_020840938.1	transcription factor IIIB 50 kDa subunit

Pathways from BioSystems
Gene Ontology

The following BRF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPh254058	XM_020985279.1 Latest version!	Phascolarctos cinereus BRF2, RNA polymerase III transcription initiation factor subunit (BRF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRF2 ( XM_020985279.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020840938.1 Phascolarctos cinereus BRF2, RNA polymerase III transcription initiation factor subunit (BRF2), mRNA.

Price & Availability↑

CloneID	OPh254058
Clone ID Related Accession (Same CDS sequence)	XM_020985279.1
Accession Version	XM_020985279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	transcription factor IIIB 50 kDa subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018343972.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGTG GGGGCCGCAG CCGCTGCCCG GACTGCGGCT GCGCGGAGCT CGTGGAGGAC 
TCGCACTACG CGCAGAGCCA GCTGGTGTGC GCCGAGTGCG GCTGCGTGGT GTCCGAGGGG 
CTGCTCACCA CCACCTTTGC GGACGAGGGC AACCTCCGAG AAGTGACGTA TTCCAGGAGT 
ACAGGGGAGA ATGAACGGGT CAGCCGCAGC CAGCAAAGAG GGCTTCGACG AGTTCGGGAT 
CTCTGCCGAG TTCTGCAGCT ACCATCAACC TTTGAGGAGA CCGCAGTGTC CTACTACCAA 
CGAGCATACC AACACCCTGG CCTCCATGCT GCTAGACTCC AAAAGAAAGA AGTGTTGGTG 
GGCTGCTGTG TGCTGGTCAC CTGCCGACAA CATAATTGGC CCCTGACCAT GGGCACTATT 
TGCACCCTGT TGTATGCCAA CTTAGAACTG TTCTCTGGCA CATACCTACA AATAGTGAAG 
GTCCTAGGAC TAGATGTTCC TGCCTTGAGC TTAGCAGACC TAGTGAAGAC TTACTGTAGC 
AGCTTCAAAC TGTTCCAGCC CTCCCCCTCT GTATCAGTCA GTTTTGTGGA GGACAAAGAG 
AAGATGGTAT CCCGGACATT ACAGCTGGTA GAGCTGGCCA GTGAATCGTG GCTGGTGACA 
GGAAGGCACC CTGTGCCGGT GATAACTGCT GCTGCTTACC TGGCCTGGAG ATCGCTGCAG 
CCTGGGAGCC GCTTGACCTG CTCCCTTTCC CGATTCTGCA AGTTGGCAGG TGTTGACCTG 
CCTCATCCAG CATACCTTCG AGTTCAGGAA CTGCTAGCCA TCCTCTTGCG GATGGCTGGG 
CAGCTGGCCT GGCTGCGGAT ACTGAACTTA GATAAGCAAA CAGTCGTGAA GCATATTGGG 
GACTTGCTCC AGCACCGGTG CATTCTCCTC CGCCAGGCTT TTCGGGATGG AGCTGAAGAG 
GAAGAAGGAG AAGAGCAGCC ACAAGAGGAG GGGGAGGAGG CCGGGAGCAG TACCTTAGAG 
GTCCTTGAAA AGAAAAGGCC AGCTAGCTCG CCACCACCTC TGCTCCCTCC CTGCATGCTG 
AGGCCTCCAA AGCGGCCCTG TCCCACTCCT GCTGATCCCA GTATTACTGG GGATGAGGAT 
ATATCTGACA GTGAGATAGA GCAGTATTTG CGCACACCTC GAGAAATCAA AGACTTCCAG 
AGAGCCCAGG CTTCGCGTCA AGCTGTCCCA GGTGCCCCTG CCCCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020840938.1
CDS	160..1410
Translation	MSGGGRSRCPDCGCAELVEDSHYAQSQLVCAECGCVVSEGLLTTTFADEGNLREVTYSRSTGENERVSRSQQRGLRRVRDLCRVLQLPSTFEETAVSYYQRAYQHPGLHAARLQKKEVLVGCCVLVTCRQHNWPLTMGTICTLLYANLELFSGTYLQIVKVLGLDVPALSLADLVKTYCSSFKLFQPSPSVSVSFVEDKEKMVSRTLQLVELASESWLVTGRHPVPVITAAAYLAWRSLQPGSRLTCSLSRFCKLAGVDLPHPAYLRVQELLAILLRMAGQLAWLRILNLDKQTVVKHIGDLLQHRCILLRQAFRDGAEEEEGEEQPQEEGEEAGSSTLEVLEKKRPASSPPPLLPPCMLRPPKRPCPTPADPSITGDEDISDSEIEQYLRTPREIKDFQRAQASRQAVPGAPAPS

Target ORF information:

RefSeq Version	XM_020985279.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus BRF2, RNA polymerase III transcription initiation factor subunit (BRF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020985279.1

ORF Insert Sequence:

ATGTCGGGTG GGGGCCGCAG CCGCTGCCCG GACTGCGGCT GCGCGGAGCT CGTGGAGGAC 
TCGCACTACG CGCAGAGCCA GCTGGTGTGC GCCGAGTGCG GCTGCGTGGT GTCCGAGGGG 
CTGCTCACCA CCACCTTTGC GGACGAGGGC AACCTCCGAG AAGTGACGTA TTCCAGGAGT 
ACAGGGGAGA ATGAACGGGT CAGCCGCAGC CAGCAAAGAG GGCTTCGACG AGTTCGGGAT 
CTCTGCCGAG TTCTGCAGCT ACCATCAACC TTTGAGGAGA CCGCAGTGTC CTACTACCAA 
CGAGCATACC AACACCCTGG CCTCCATGCT GCTAGACTCC AAAAGAAAGA AGTGTTGGTG 
GGCTGCTGTG TGCTGGTCAC CTGCCGACAA CATAATTGGC CCCTGACCAT GGGCACTATT 
TGCACCCTGT TGTATGCCAA CTTAGAACTG TTCTCTGGCA CATACCTACA AATAGTGAAG 
GTCCTAGGAC TAGATGTTCC TGCCTTGAGC TTAGCAGACC TAGTGAAGAC TTACTGTAGC 
AGCTTCAAAC TGTTCCAGCC CTCCCCCTCT GTATCAGTCA GTTTTGTGGA GGACAAAGAG 
AAGATGGTAT CCCGGACATT ACAGCTGGTA GAGCTGGCCA GTGAATCGTG GCTGGTGACA 
GGAAGGCACC CTGTGCCGGT GATAACTGCT GCTGCTTACC TGGCCTGGAG ATCGCTGCAG 
CCTGGGAGCC GCTTGACCTG CTCCCTTTCC CGATTCTGCA AGTTGGCAGG TGTTGACCTG 
CCTCATCCAG CATACCTTCG AGTTCAGGAA CTGCTAGCCA TCCTCTTGCG GATGGCTGGG 
CAGCTGGCCT GGCTGCGGAT ACTGAACTTA GATAAGCAAA CAGTCGTGAA GCATATTGGG 
GACTTGCTCC AGCACCGGTG CATTCTCCTC CGCCAGGCTT TTCGGGATGG AGCTGAAGAG 
GAAGAAGGAG AAGAGCAGCC ACAAGAGGAG GGGGAGGAGG CCGGGAGCAG TACCTTAGAG 
GTCCTTGAAA AGAAAAGGCC AGCTAGCTCG CCACCACCTC TGCTCCCTCC CTGCATGCTG 
AGGCCTCCAA AGCGGCCCTG TCCCACTCCT GCTGATCCCA GTATTACTGG GGATGAGGAT 
ATATCTGACA GTGAGATAGA GCAGTATTTG CGCACACCTC GAGAAATCAA AGACTTCCAG 
AGAGCCCAGG CTTCGCGTCA AGCTGTCCCA GGTGCCCCTG CCCCTTCCTG A