HELB cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	HELB
Entrez Gene ID	102460049
Full Name	DNA helicase B
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025188263.1	XP_025044048.1	DNA helicase B
XM_014577081.1	XP_014432567.1	DNA helicase B

Pathways from BioSystems
Gene Ontology

The following HELB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HELB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPe35764	XM_025188263.1 Latest version!	Pelodiscus sinensis DNA helicase B (HELB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPe35764	XM_014577081.1 Latest version!	Pelodiscus sinensis helicase (DNA) B (HELB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HELB ( XM_025188263.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_025044048.1 Pelodiscus sinensis DNA helicase B (HELB), mRNA.

Price & Availability↑

CloneID	OPe35764
Clone ID Related Accession (Same CDS sequence)	XM_025188263.1 , XM_014577081.1
Accession Version	XM_025188263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	DNA helicase B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005857030.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATAACG TGGTGGGCAA TTTTCCATTA GTCGGCGCCT GGTGGAAAGT TCATGTTAAA 
GTTAAATGCG TGCAATCGAA GTATTTTGTG CAAGGATATC CATCCTACTT CCTACGAACT 
GATATAAGGG AAAACCAAAA TGCAGTTTTT TCACTCTTTT TTAACGAGTG TGGTGTTCCT 
GATTCTTTCA TAAAAGAATT TATGAGTTTT CCTCAGAGAC CTGTCCTGAA TTTCAGAAAC 
CTTGAAGAAA AATTAAATCA GTTTCAAGAG TCAAAATTAA ATCTTCAGAA AGGGAACAAA 
TCAATGAAAA CCAAAGATTT TGATATTTTT TATTATATTA TGCAGTCATT TACAGGAAAG 
ACTGCTTTGG TAGCTTTGAA TTTCCCAATG ATAATGGAGT TCCTGCCAAG ACTTCTGCCA 
TATCATTTTT GCAATATCAT AAAGGGTGTG AGCTGGGTTA GAGTGACAAA ACAGAACAGC 
AAAACTGATG AAAAAGCTTT TCCTCAAAAT GAGATACTAC CAAAATTAGA TGAGATGTTA 
AAAAAAGAAC CATGGAAACT TGGATTCAGC AGGATCACCT ACAGAGAATT GAACGTTTCC 
AATTGTGAGG CAACTTGGAC AGCCTTCTGT CAGTGTGAAC ATCTCCTCTC GAAGATTCCT 
GAATTGCAGA AGAATGCATT AATAATATAC AATAAACTCA AGCAAAAATG TAGAGAAATG 
GGACACACCT ATGAAGAACA AGACATTTTG GACTCTCTGG TTTTTGAAGA TATGCCCATT 
GAGCATGCCT GGGAGTCCTT GAAATTTTTG AAAGATGAGA TGATAGTGAT TGGTGAGAAG 
AAACTGGTAT TTCTTTCTCA CCTTTACAAA TCTGAAAAAA ACATTGCACT TTCTGTAAAT 
CATCTAATGA CAAATGACCC ATGGCAGTTA TGTGTTAATG CAAGAGAAGT ACTTAATGTT 
AGATATGCTA ACAAATCAAA AGCAAATGGA GATATTAACA TAAACAATAA CCAGCTTTAT 
GAAACAGAGC AAGTGTTAAA CCCAGACATA CAAAACTCCT CAGATATACA AAACATTGGT 
GACAGTGATT TTCTTTATAA TGAAAAAGTA TCAGATATAG AAACTACAAG TGAAGCAGAA 
GTGGACCCAG ATCAGTTGAA AGCTATGGAA ATGATTTGTT CTAATCCAGT GACAATCATC 
AGTGGAAAAG GGGGTTGTGG GAAGACTACT ATAGTTTGTT GCCTTTTTCA GTATTTAAAG 
CAGTTGGAAA AAGAAGTGGT AAGGGCTTGT AATGATTTTG AAAAGGACCT GGATGCATCT 
TCTGAATGGA GTACTTCTCA TCATTTTTGC CCAGAGGATA TTTGCCCAAG AAACCCTTTA 
AATGTTTTAC TTACTGCACC TACAGGAAAG GCAGCTAGTC TCCTGAATGA GAAAACTGAG 
CTTCCTGCCT ATACTCTCCA TCAGGTCATC TACAGTTTTT ATTCATGGAT GTTAAGAGAT 
GGACCAACAC CATGGAAGTT TTCTAATGTG ACAGTTTTGG TTGTGGATGA AGGGAGCTTA 
GTATCTGTAC AAAAGCTCAG TTCAGTTTTA AAATTGCTGT GTGACCATGC TCAGCTTGCC 
AAGCTCATTA TTCTTGGTGA TATTAGACAG TTACCCAGCA TTGACCCTGG CAATATGCTC 
GCTGATATTT TTGAGGCTCT AAAATCTAGA GGCTTGTCTG TGGAACTAAG AACTAATCAT 
AGAGCTGAAT CTCAGCTAAT TGTGGATAAT GCCACAAGAA TCTCTCAGCG AAAATTCCCA 
GTATTTGATT CGGTACACAA AGTCTGTGAT TGGAATGAAA AGTTGATCAT GCCAGGCCCT 
GAGAAAAAAT TTATTTTCAT TGTTTTGCCT TCTGGAGACA ATGGTCAATA CTTGCAATCT 
GCCATAAAAA CTTTACTTGA AAAAGGACCA GGACTGCAAG ATGCCAAAGA ATCACAGTTC 
ATTGCTTTCA GAAGGCAAGA TTGTGATCTA ATAAATGAGC TCTGCTGTCT GCACTATTCA 
AAACATTCAA TCAGAAATCC TAAAAATCGC CTGTCGTTCC AATGTGATGA CAAAATTTGT 
TCCACGAGGA ATGCCTATCT CAAGGACCTT CTTCCAGAAA ATAACACTGA TTGCACAAGG 
AATAGTGACT CTTCGACTCC AGATCAATGT GTATGCAGGG GGTGTTGTAG AAGAAAAGCC 
TTTCTCAGTG GAGAAAAGCT ACCCAGTGAT TTTGGGGGCA GCGTTGAGGA TGACAGACGA 
CTCTGCAATG GAGAAATATT TTTCATAATA GATGATGTGG AAGTTGACAG ATGTCGTTTA 
TTAACCATCA GCAGCACATA TGGTTCCGAA TACACTGTGA GGTATAAGGC GCTGAGGAGA 
GAGTGTCATA TAAAGCATGC ATGGGCCAGA ACTATTCACA CTTTTCAGGG TTCAGAGGAA 
AAAACAGTTG TCTATGTAGT CGGGAATGCA GGCCGACAGC ACTGGCAGCA TGTTTACACA 
GCTGTGACTC GAGGCCGCTG TCGGGTCTAT ATTGTGGCTG AGCAGCCTCA ACTCAGGAAG 
GCAGTTACTA CGAAGAGCTT TCCCAGAAAA ACTCGCTTAC AACAACGTTT GAGAGAGGTC 
TTTGCATTCA GTGACTCTAC TGAACAAATG TCCTCTCAAC TACAGGATTG TTGGCAAAGT 
CAAGGACGTG CTCCTCAACC TGGTCCTATT TCAGTAGCTC AGGATATTCC CCTCTCTTCT 
TTAGCTGTTG AAGACGACTT CACTGAAGCA GAAGAACCTG CAATTCTTCA AAATGTACAA 
ACTAACAATG AACAGTGCAG TACTGGTGAA CTGGCCAGTG CAGGCGGTGA AACTCCCCTT 
AAATCAAGTG GATGTAAAAG ACAAAGCAGT TTTACGGATA GTTTTGAAAG TCCTTCAAAA 
GTTACCATGG TAAATCCAGA AGATTCGCCC CTTGGGTCAG TCAGACTTCA GAATCTGACT 
TTGAAGTGCC CAACTCCCAA GCAGCTTTTT GGACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025044048.1
CDS	49..3087
Translation	MYNVVGNFPLVGAWWKVHVKVKCVQSKYFVQGYPSYFLRTDIRENQNAVFSLFFNECGVPDSFIKEFMSFPQRPVLNFRNLEEKLNQFQESKLNLQKGNKSMKTKDFDIFYYIMQSFTGKTALVALNFPMIMEFLPRLLPYHFCNIIKGVSWVRVTKQNSKTDEKAFPQNEILPKLDEMLKKEPWKLGFSRITYRELNVSNCEATWTAFCQCEHLLSKIPELQKNALIIYNKLKQKCREMGHTYEEQDILDSLVFEDMPIEHAWESLKFLKDEMIVIGEKKLVFLSHLYKSEKNIALSVNHLMTNDPWQLCVNAREVLNVRYANKSKANGDININNNQLYETEQVLNPDIQNSSDIQNIGDSDFLYNEKVSDIETTSEAEVDPDQLKAMEMICSNPVTIISGKGGCGKTTIVCCLFQYLKQLEKEVVRACNDFEKDLDASSEWSTSHHFCPEDICPRNPLNVLLTAPTGKAASLLNEKTELPAYTLHQVIYSFYSWMLRDGPTPWKFSNVTVLVVDEGSLVSVQKLSSVLKLLCDHAQLAKLIILGDIRQLPSIDPGNMLADIFEALKSRGLSVELRTNHRAESQLIVDNATRISQRKFPVFDSVHKVCDWNEKLIMPGPEKKFIFIVLPSGDNGQYLQSAIKTLLEKGPGLQDAKESQFIAFRRQDCDLINELCCLHYSKHSIRNPKNRLSFQCDDKICSTRNAYLKDLLPENNTDCTRNSDSSTPDQCVCRGCCRRKAFLSGEKLPSDFGGSVEDDRRLCNGEIFFIIDDVEVDRCRLLTISSTYGSEYTVRYKALRRECHIKHAWARTIHTFQGSEEKTVVYVVGNAGRQHWQHVYTAVTRGRCRVYIVAEQPQLRKAVTTKSFPRKTRLQQRLREVFAFSDSTEQMSSQLQDCWQSQGRAPQPGPISVAQDIPLSSLAVEDDFTEAEEPAILQNVQTNNEQCSTGELASAGGETPLKSSGCKRQSSFTDSFESPSKVTMVNPEDSPLGSVRLQNLTLKCPTPKQLFGP

Target ORF information:

RefSeq Version	XM_025188263.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis DNA helicase B (HELB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025188263.1

ORF Insert Sequence:

ATGTATAACG TGGTGGGCAA TTTTCCATTA GTCGGCGCCT GGTGGAAAGT TCATGTTAAA 
GTTAAATGCG TGCAATCGAA GTATTTTGTG CAAGGATATC CATCCTACTT CCTACGAACT 
GATATAAGGG AAAACCAAAA TGCAGTTTTT TCACTCTTTT TTAACGAGTG TGGTGTTCCT 
GATTCTTTCA TAAAAGAATT TATGAGTTTT CCTCAGAGAC CTGTCCTGAA TTTCAGAAAC 
CTTGAAGAAA AATTAAATCA GTTTCAAGAG TCAAAATTAA ATCTTCAGAA AGGGAACAAA 
TCAATGAAAA CCAAAGATTT TGATATTTTT TATTATATTA TGCAGTCATT TACAGGAAAG 
ACTGCTTTGG TAGCTTTGAA TTTCCCAATG ATAATGGAGT TCCTGCCAAG ACTTCTGCCA 
TATCATTTTT GCAATATCAT AAAGGGTGTG AGCTGGGTTA GAGTGACAAA ACAGAACAGC 
AAAACTGATG AAAAAGCTTT TCCTCAAAAT GAGATACTAC CAAAATTAGA TGAGATGTTA 
AAAAAAGAAC CATGGAAACT TGGATTCAGC AGGATCACCT ACAGAGAATT GAACGTTTCC 
AATTGTGAGG CAACTTGGAC AGCCTTCTGT CAGTGTGAAC ATCTCCTCTC GAAGATTCCT 
GAATTGCAGA AGAATGCATT AATAATATAC AATAAACTCA AGCAAAAATG TAGAGAAATG 
GGACACACCT ATGAAGAACA AGACATTTTG GACTCTCTGG TTTTTGAAGA TATGCCCATT 
GAGCATGCCT GGGAGTCCTT GAAATTTTTG AAAGATGAGA TGATAGTGAT TGGTGAGAAG 
AAACTGGTAT TTCTTTCTCA CCTTTACAAA TCTGAAAAAA ACATTGCACT TTCTGTAAAT 
CATCTAATGA CAAATGACCC ATGGCAGTTA TGTGTTAATG CAAGAGAAGT ACTTAATGTT 
AGATATGCTA ACAAATCAAA AGCAAATGGA GATATTAACA TAAACAATAA CCAGCTTTAT 
GAAACAGAGC AAGTGTTAAA CCCAGACATA CAAAACTCCT CAGATATACA AAACATTGGT 
GACAGTGATT TTCTTTATAA TGAAAAAGTA TCAGATATAG AAACTACAAG TGAAGCAGAA 
GTGGACCCAG ATCAGTTGAA AGCTATGGAA ATGATTTGTT CTAATCCAGT GACAATCATC 
AGTGGAAAAG GGGGTTGTGG GAAGACTACT ATAGTTTGTT GCCTTTTTCA GTATTTAAAG 
CAGTTGGAAA AAGAAGTGGT AAGGGCTTGT AATGATTTTG AAAAGGACCT GGATGCATCT 
TCTGAATGGA GTACTTCTCA TCATTTTTGC CCAGAGGATA TTTGCCCAAG AAACCCTTTA 
AATGTTTTAC TTACTGCACC TACAGGAAAG GCAGCTAGTC TCCTGAATGA GAAAACTGAG 
CTTCCTGCCT ATACTCTCCA TCAGGTCATC TACAGTTTTT ATTCATGGAT GTTAAGAGAT 
GGACCAACAC CATGGAAGTT TTCTAATGTG ACAGTTTTGG TTGTGGATGA AGGGAGCTTA 
GTATCTGTAC AAAAGCTCAG TTCAGTTTTA AAATTGCTGT GTGACCATGC TCAGCTTGCC 
AAGCTCATTA TTCTTGGTGA TATTAGACAG TTACCCAGCA TTGACCCTGG CAATATGCTC 
GCTGATATTT TTGAGGCTCT AAAATCTAGA GGCTTGTCTG TGGAACTAAG AACTAATCAT 
AGAGCTGAAT CTCAGCTAAT TGTGGATAAT GCCACAAGAA TCTCTCAGCG AAAATTCCCA 
GTATTTGATT CGGTACACAA AGTCTGTGAT TGGAATGAAA AGTTGATCAT GCCAGGCCCT 
GAGAAAAAAT TTATTTTCAT TGTTTTGCCT TCTGGAGACA ATGGTCAATA CTTGCAATCT 
GCCATAAAAA CTTTACTTGA AAAAGGACCA GGACTGCAAG ATGCCAAAGA ATCACAGTTC 
ATTGCTTTCA GAAGGCAAGA TTGTGATCTA ATAAATGAGC TCTGCTGTCT GCACTATTCA 
AAACATTCAA TCAGAAATCC TAAAAATCGC CTGTCGTTCC AATGTGATGA CAAAATTTGT 
TCCACGAGGA ATGCCTATCT CAAGGACCTT CTTCCAGAAA ATAACACTGA TTGCACAAGG 
AATAGTGACT CTTCGACTCC AGATCAATGT GTATGCAGGG GGTGTTGTAG AAGAAAAGCC 
TTTCTCAGTG GAGAAAAGCT ACCCAGTGAT TTTGGGGGCA GCGTTGAGGA TGACAGACGA 
CTCTGCAATG GAGAAATATT TTTCATAATA GATGATGTGG AAGTTGACAG ATGTCGTTTA 
TTAACCATCA GCAGCACATA TGGTTCCGAA TACACTGTGA GGTATAAGGC GCTGAGGAGA 
GAGTGTCATA TAAAGCATGC ATGGGCCAGA ACTATTCACA CTTTTCAGGG TTCAGAGGAA 
AAAACAGTTG TCTATGTAGT CGGGAATGCA GGCCGACAGC ACTGGCAGCA TGTTTACACA 
GCTGTGACTC GAGGCCGCTG TCGGGTCTAT ATTGTGGCTG AGCAGCCTCA ACTCAGGAAG 
GCAGTTACTA CGAAGAGCTT TCCCAGAAAA ACTCGCTTAC AACAACGTTT GAGAGAGGTC 
TTTGCATTCA GTGACTCTAC TGAACAAATG TCCTCTCAAC TACAGGATTG TTGGCAAAGT 
CAAGGACGTG CTCCTCAACC TGGTCCTATT TCAGTAGCTC AGGATATTCC CCTCTCTTCT 
TTAGCTGTTG AAGACGACTT CACTGAAGCA GAAGAACCTG CAATTCTTCA AAATGTACAA 
ACTAACAATG AACAGTGCAG TACTGGTGAA CTGGCCAGTG CAGGCGGTGA AACTCCCCTT 
AAATCAAGTG GATGTAAAAG ACAAAGCAGT TTTACGGATA GTTTTGAAAG TCCTTCAAAA 
GTTACCATGG TAAATCCAGA AGATTCGCCC CTTGGGTCAG TCAGACTTCA GAATCTGACT 
TTGAAGTGCC CAACTCCCAA GCAGCTTTTT GGACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HELB ( XM_014577081.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014432567.1 Pelodiscus sinensis helicase (DNA) B (HELB), mRNA.

Price & Availability↑

CloneID	OPe35764
Clone ID Related Accession (Same CDS sequence)	XM_025188263.1 , XM_014577081.1
Accession Version	XM_014577081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	DNA helicase B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005857030.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATAACG TGGTGGGCAA TTTTCCATTA GTCGGCGCCT GGTGGAAAGT TCATGTTAAA 
GTTAAATGCG TGCAATCGAA GTATTTTGTG CAAGGATATC CATCCTACTT CCTACGAACT 
GATATAAGGG AAAACCAAAA TGCAGTTTTT TCACTCTTTT TTAACGAGTG TGGTGTTCCT 
GATTCTTTCA TAAAAGAATT TATGAGTTTT CCTCAGAGAC CTGTCCTGAA TTTCAGAAAC 
CTTGAAGAAA AATTAAATCA GTTTCAAGAG TCAAAATTAA ATCTTCAGAA AGGGAACAAA 
TCAATGAAAA CCAAAGATTT TGATATTTTT TATTATATTA TGCAGTCATT TACAGGAAAG 
ACTGCTTTGG TAGCTTTGAA TTTCCCAATG ATAATGGAGT TCCTGCCAAG ACTTCTGCCA 
TATCATTTTT GCAATATCAT AAAGGGTGTG AGCTGGGTTA GAGTGACAAA ACAGAACAGC 
AAAACTGATG AAAAAGCTTT TCCTCAAAAT GAGATACTAC CAAAATTAGA TGAGATGTTA 
AAAAAAGAAC CATGGAAACT TGGATTCAGC AGGATCACCT ACAGAGAATT GAACGTTTCC 
AATTGTGAGG CAACTTGGAC AGCCTTCTGT CAGTGTGAAC ATCTCCTCTC GAAGATTCCT 
GAATTGCAGA AGAATGCATT AATAATATAC AATAAACTCA AGCAAAAATG TAGAGAAATG 
GGACACACCT ATGAAGAACA AGACATTTTG GACTCTCTGG TTTTTGAAGA TATGCCCATT 
GAGCATGCCT GGGAGTCCTT GAAATTTTTG AAAGATGAGA TGATAGTGAT TGGTGAGAAG 
AAACTGGTAT TTCTTTCTCA CCTTTACAAA TCTGAAAAAA ACATTGCACT TTCTGTAAAT 
CATCTAATGA CAAATGACCC ATGGCAGTTA TGTGTTAATG CAAGAGAAGT ACTTAATGTT 
AGATATGCTA ACAAATCAAA AGCAAATGGA GATATTAACA TAAACAATAA CCAGCTTTAT 
GAAACAGAGC AAGTGTTAAA CCCAGACATA CAAAACTCCT CAGATATACA AAACATTGGT 
GACAGTGATT TTCTTTATAA TGAAAAAGTA TCAGATATAG AAACTACAAG TGAAGCAGAA 
GTGGACCCAG ATCAGTTGAA AGCTATGGAA ATGATTTGTT CTAATCCAGT GACAATCATC 
AGTGGAAAAG GGGGTTGTGG GAAGACTACT ATAGTTTGTT GCCTTTTTCA GTATTTAAAG 
CAGTTGGAAA AAGAAGTGGT AAGGGCTTGT AATGATTTTG AAAAGGACCT GGATGCATCT 
TCTGAATGGA GTACTTCTCA TCATTTTTGC CCAGAGGATA TTTGCCCAAG AAACCCTTTA 
AATGTTTTAC TTACTGCACC TACAGGAAAG GCAGCTAGTC TCCTGAATGA GAAAACTGAG 
CTTCCTGCCT ATACTCTCCA TCAGGTCATC TACAGTTTTT ATTCATGGAT GTTAAGAGAT 
GGACCAACAC CATGGAAGTT TTCTAATGTG ACAGTTTTGG TTGTGGATGA AGGGAGCTTA 
GTATCTGTAC AAAAGCTCAG TTCAGTTTTA AAATTGCTGT GTGACCATGC TCAGCTTGCC 
AAGCTCATTA TTCTTGGTGA TATTAGACAG TTACCCAGCA TTGACCCTGG CAATATGCTC 
GCTGATATTT TTGAGGCTCT AAAATCTAGA GGCTTGTCTG TGGAACTAAG AACTAATCAT 
AGAGCTGAAT CTCAGCTAAT TGTGGATAAT GCCACAAGAA TCTCTCAGCG AAAATTCCCA 
GTATTTGATT CGGTACACAA AGTCTGTGAT TGGAATGAAA AGTTGATCAT GCCAGGCCCT 
GAGAAAAAAT TTATTTTCAT TGTTTTGCCT TCTGGAGACA ATGGTCAATA CTTGCAATCT 
GCCATAAAAA CTTTACTTGA AAAAGGACCA GGACTGCAAG ATGCCAAAGA ATCACAGTTC 
ATTGCTTTCA GAAGGCAAGA TTGTGATCTA ATAAATGAGC TCTGCTGTCT GCACTATTCA 
AAACATTCAA TCAGAAATCC TAAAAATCGC CTGTCGTTCC AATGTGATGA CAAAATTTGT 
TCCACGAGGA ATGCCTATCT CAAGGACCTT CTTCCAGAAA ATAACACTGA TTGCACAAGG 
AATAGTGACT CTTCGACTCC AGATCAATGT GTATGCAGGG GGTGTTGTAG AAGAAAAGCC 
TTTCTCAGTG GAGAAAAGCT ACCCAGTGAT TTTGGGGGCA GCGTTGAGGA TGACAGACGA 
CTCTGCAATG GAGAAATATT TTTCATAATA GATGATGTGG AAGTTGACAG ATGTCGTTTA 
TTAACCATCA GCAGCACATA TGGTTCCGAA TACACTGTGA GGTATAAGGC GCTGAGGAGA 
GAGTGTCATA TAAAGCATGC ATGGGCCAGA ACTATTCACA CTTTTCAGGG TTCAGAGGAA 
AAAACAGTTG TCTATGTAGT CGGGAATGCA GGCCGACAGC ACTGGCAGCA TGTTTACACA 
GCTGTGACTC GAGGCCGCTG TCGGGTCTAT ATTGTGGCTG AGCAGCCTCA ACTCAGGAAG 
GCAGTTACTA CGAAGAGCTT TCCCAGAAAA ACTCGCTTAC AACAACGTTT GAGAGAGGTC 
TTTGCATTCA GTGACTCTAC TGAACAAATG TCCTCTCAAC TACAGGATTG TTGGCAAAGT 
CAAGGACGTG CTCCTCAACC TGGTCCTATT TCAGTAGCTC AGGATATTCC CCTCTCTTCT 
TTAGCTGTTG AAGACGACTT CACTGAAGCA GAAGAACCTG CAATTCTTCA AAATGTACAA 
ACTAACAATG AACAGTGCAG TACTGGTGAA CTGGCCAGTG CAGGCGGTGA AACTCCCCTT 
AAATCAAGTG GATGTAAAAG ACAAAGCAGT TTTACGGATA GTTTTGAAAG TCCTTCAAAA 
GTTACCATGG TAAATCCAGA AGATTCGCCC CTTGGGTCAG TCAGACTTCA GAATCTGACT 
TTGAAGTGCC CAACTCCCAA GCAGCTTTTT GGACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014432567.1
CDS	173..3211
Translation	MYNVVGNFPLVGAWWKVHVKVKCVQSKYFVQGYPSYFLRTDIRENQNAVFSLFFNECGVPDSFIKEFMSFPQRPVLNFRNLEEKLNQFQESKLNLQKGNKSMKTKDFDIFYYIMQSFTGKTALVALNFPMIMEFLPRLLPYHFCNIIKGVSWVRVTKQNSKTDEKAFPQNEILPKLDEMLKKEPWKLGFSRITYRELNVSNCEATWTAFCQCEHLLSKIPELQKNALIIYNKLKQKCREMGHTYEEQDILDSLVFEDMPIEHAWESLKFLKDEMIVIGEKKLVFLSHLYKSEKNIALSVNHLMTNDPWQLCVNAREVLNVRYANKSKANGDININNNQLYETEQVLNPDIQNSSDIQNIGDSDFLYNEKVSDIETTSEAEVDPDQLKAMEMICSNPVTIISGKGGCGKTTIVCCLFQYLKQLEKEVVRACNDFEKDLDASSEWSTSHHFCPEDICPRNPLNVLLTAPTGKAASLLNEKTELPAYTLHQVIYSFYSWMLRDGPTPWKFSNVTVLVVDEGSLVSVQKLSSVLKLLCDHAQLAKLIILGDIRQLPSIDPGNMLADIFEALKSRGLSVELRTNHRAESQLIVDNATRISQRKFPVFDSVHKVCDWNEKLIMPGPEKKFIFIVLPSGDNGQYLQSAIKTLLEKGPGLQDAKESQFIAFRRQDCDLINELCCLHYSKHSIRNPKNRLSFQCDDKICSTRNAYLKDLLPENNTDCTRNSDSSTPDQCVCRGCCRRKAFLSGEKLPSDFGGSVEDDRRLCNGEIFFIIDDVEVDRCRLLTISSTYGSEYTVRYKALRRECHIKHAWARTIHTFQGSEEKTVVYVVGNAGRQHWQHVYTAVTRGRCRVYIVAEQPQLRKAVTTKSFPRKTRLQQRLREVFAFSDSTEQMSSQLQDCWQSQGRAPQPGPISVAQDIPLSSLAVEDDFTEAEEPAILQNVQTNNEQCSTGELASAGGETPLKSSGCKRQSSFTDSFESPSKVTMVNPEDSPLGSVRLQNLTLKCPTPKQLFGP

Target ORF information:

RefSeq Version	XM_014577081.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis helicase (DNA) B (HELB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014577081.1

ORF Insert Sequence:

ATGTATAACG TGGTGGGCAA TTTTCCATTA GTCGGCGCCT GGTGGAAAGT TCATGTTAAA 
GTTAAATGCG TGCAATCGAA GTATTTTGTG CAAGGATATC CATCCTACTT CCTACGAACT 
GATATAAGGG AAAACCAAAA TGCAGTTTTT TCACTCTTTT TTAACGAGTG TGGTGTTCCT 
GATTCTTTCA TAAAAGAATT TATGAGTTTT CCTCAGAGAC CTGTCCTGAA TTTCAGAAAC 
CTTGAAGAAA AATTAAATCA GTTTCAAGAG TCAAAATTAA ATCTTCAGAA AGGGAACAAA 
TCAATGAAAA CCAAAGATTT TGATATTTTT TATTATATTA TGCAGTCATT TACAGGAAAG 
ACTGCTTTGG TAGCTTTGAA TTTCCCAATG ATAATGGAGT TCCTGCCAAG ACTTCTGCCA 
TATCATTTTT GCAATATCAT AAAGGGTGTG AGCTGGGTTA GAGTGACAAA ACAGAACAGC 
AAAACTGATG AAAAAGCTTT TCCTCAAAAT GAGATACTAC CAAAATTAGA TGAGATGTTA 
AAAAAAGAAC CATGGAAACT TGGATTCAGC AGGATCACCT ACAGAGAATT GAACGTTTCC 
AATTGTGAGG CAACTTGGAC AGCCTTCTGT CAGTGTGAAC ATCTCCTCTC GAAGATTCCT 
GAATTGCAGA AGAATGCATT AATAATATAC AATAAACTCA AGCAAAAATG TAGAGAAATG 
GGACACACCT ATGAAGAACA AGACATTTTG GACTCTCTGG TTTTTGAAGA TATGCCCATT 
GAGCATGCCT GGGAGTCCTT GAAATTTTTG AAAGATGAGA TGATAGTGAT TGGTGAGAAG 
AAACTGGTAT TTCTTTCTCA CCTTTACAAA TCTGAAAAAA ACATTGCACT TTCTGTAAAT 
CATCTAATGA CAAATGACCC ATGGCAGTTA TGTGTTAATG CAAGAGAAGT ACTTAATGTT 
AGATATGCTA ACAAATCAAA AGCAAATGGA GATATTAACA TAAACAATAA CCAGCTTTAT 
GAAACAGAGC AAGTGTTAAA CCCAGACATA CAAAACTCCT CAGATATACA AAACATTGGT 
GACAGTGATT TTCTTTATAA TGAAAAAGTA TCAGATATAG AAACTACAAG TGAAGCAGAA 
GTGGACCCAG ATCAGTTGAA AGCTATGGAA ATGATTTGTT CTAATCCAGT GACAATCATC 
AGTGGAAAAG GGGGTTGTGG GAAGACTACT ATAGTTTGTT GCCTTTTTCA GTATTTAAAG 
CAGTTGGAAA AAGAAGTGGT AAGGGCTTGT AATGATTTTG AAAAGGACCT GGATGCATCT 
TCTGAATGGA GTACTTCTCA TCATTTTTGC CCAGAGGATA TTTGCCCAAG AAACCCTTTA 
AATGTTTTAC TTACTGCACC TACAGGAAAG GCAGCTAGTC TCCTGAATGA GAAAACTGAG 
CTTCCTGCCT ATACTCTCCA TCAGGTCATC TACAGTTTTT ATTCATGGAT GTTAAGAGAT 
GGACCAACAC CATGGAAGTT TTCTAATGTG ACAGTTTTGG TTGTGGATGA AGGGAGCTTA 
GTATCTGTAC AAAAGCTCAG TTCAGTTTTA AAATTGCTGT GTGACCATGC TCAGCTTGCC 
AAGCTCATTA TTCTTGGTGA TATTAGACAG TTACCCAGCA TTGACCCTGG CAATATGCTC 
GCTGATATTT TTGAGGCTCT AAAATCTAGA GGCTTGTCTG TGGAACTAAG AACTAATCAT 
AGAGCTGAAT CTCAGCTAAT TGTGGATAAT GCCACAAGAA TCTCTCAGCG AAAATTCCCA 
GTATTTGATT CGGTACACAA AGTCTGTGAT TGGAATGAAA AGTTGATCAT GCCAGGCCCT 
GAGAAAAAAT TTATTTTCAT TGTTTTGCCT TCTGGAGACA ATGGTCAATA CTTGCAATCT 
GCCATAAAAA CTTTACTTGA AAAAGGACCA GGACTGCAAG ATGCCAAAGA ATCACAGTTC 
ATTGCTTTCA GAAGGCAAGA TTGTGATCTA ATAAATGAGC TCTGCTGTCT GCACTATTCA 
AAACATTCAA TCAGAAATCC TAAAAATCGC CTGTCGTTCC AATGTGATGA CAAAATTTGT 
TCCACGAGGA ATGCCTATCT CAAGGACCTT CTTCCAGAAA ATAACACTGA TTGCACAAGG 
AATAGTGACT CTTCGACTCC AGATCAATGT GTATGCAGGG GGTGTTGTAG AAGAAAAGCC 
TTTCTCAGTG GAGAAAAGCT ACCCAGTGAT TTTGGGGGCA GCGTTGAGGA TGACAGACGA 
CTCTGCAATG GAGAAATATT TTTCATAATA GATGATGTGG AAGTTGACAG ATGTCGTTTA 
TTAACCATCA GCAGCACATA TGGTTCCGAA TACACTGTGA GGTATAAGGC GCTGAGGAGA 
GAGTGTCATA TAAAGCATGC ATGGGCCAGA ACTATTCACA CTTTTCAGGG TTCAGAGGAA 
AAAACAGTTG TCTATGTAGT CGGGAATGCA GGCCGACAGC ACTGGCAGCA TGTTTACACA 
GCTGTGACTC GAGGCCGCTG TCGGGTCTAT ATTGTGGCTG AGCAGCCTCA ACTCAGGAAG 
GCAGTTACTA CGAAGAGCTT TCCCAGAAAA ACTCGCTTAC AACAACGTTT GAGAGAGGTC 
TTTGCATTCA GTGACTCTAC TGAACAAATG TCCTCTCAAC TACAGGATTG TTGGCAAAGT 
CAAGGACGTG CTCCTCAACC TGGTCCTATT TCAGTAGCTC AGGATATTCC CCTCTCTTCT 
TTAGCTGTTG AAGACGACTT CACTGAAGCA GAAGAACCTG CAATTCTTCA AAATGTACAA 
ACTAACAATG AACAGTGCAG TACTGGTGAA CTGGCCAGTG CAGGCGGTGA AACTCCCCTT 
AAATCAAGTG GATGTAAAAG ACAAAGCAGT TTTACGGATA GTTTTGAAAG TCCTTCAAAA 
GTTACCATGG TAAATCCAGA AGATTCGCCC CTTGGGTCAG TCAGACTTCA GAATCTGACT 
TTGAAGTGCC CAACTCCCAA GCAGCTTTTT GGACCTTAA