GUSB cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUSB
Entrez Gene ID	102458648
Full Name	glucuronidase beta
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025179721.1	XP_025035506.1	beta-glucuronidase
XM_006113113.2	XP_006113175.1	beta-glucuronidase

Pathways from BioSystems
Gene Ontology

The following GUSB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUSB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPe20163	XM_006113113.2 Latest version!	Pelodiscus sinensis glucuronidase, beta (GUSB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUSB ( XM_006113113.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006113175.1 Pelodiscus sinensis glucuronidase, beta (GUSB), mRNA.

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CloneID	OPe20163
Clone ID Related Accession (Same CDS sequence)	XM_006113113.2
Accession Version	XM_006113113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	beta-glucuronidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005852190.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 3, 2015 this sequence version replaced XM_006113113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGCGTCCCC ACGCAAACCG TTTCACCCTC TGTCTCCCTT GCTCCTGCCA GAGTGGGCCT 
GTGATCGACA TGCCGGTACC GGCCAGCTTC AACGAGCTGA CTCAGGATCC CAATCTGCGG 
CAATATGTTG GCTGGGTTTG GTATGAGAGA GAGGTGCTGC TTCCCCGGAG CTGGCTGCTG 
AATGCCCTCA CCACCCGAGT CGTGCTGCGG GTGGGCAGTG CACATTACTA CACCATAGTC 
TGGGTCAATG GAGTGCAGGT GACGGAGCAT GAAGGGGGCC ACCTGCCCTT CGAAGCCGAC 
ATAAGCAGGA TTGTGCAGGC TGGCCCCGGG GCCCTGTGCC GCATCACCGT TGCTCTCAAC 
AACACCCTGA CGCCTCACAC CCTGCCTCCC GGCGCCATTC AGTTCCTGGC GGACGAGTCT 
AAGTATCCCA AGGGCTATTT TGTTCAGAAC ACAAAGTTTG ATTTCTTTAA CTACGCTGGA 
ATTCACCGTC CGGTCGTGCT GTATACAACC CCCACGGTCT ACATAGATGA CATCACTGTG 
ACTACAGCCT GGACAGGGAA TATTGGTGTG GTGCGGTATC AGGTGTTGGT GGCCGGCAGC 
ACCCACTACT CCTTGTCGCT GTCTCTCCGT GAGAAAGAGG GGAAAGTAGT TGCTACGGGA 
AATGGAACAG TCGGAGAGTT GAAAGTAGTG GATCCAAAGC TTTGGTGGCC TTATCTGATG 
CACGAAAACC CTGGATACCT TTATTACTTG GAGGTGACGC TGTTGGCGCA GGCGGGCGGG 
GAGTCGCTGG AAGATTTGTA CACGCTCCCC GTCGGGATCC GCACCGTACA TGTCACCCGC 
AGCCAGTTCC TCATCAACGG AAAGCCCTTC TACTTCCATG GCGTCAACAA GCACGAGGAT 
GCTGACATCC GCGGCAAAGG CCTGGACTGG TCACTGATTG TGAAGGATTT CAACCTACTG 
CGGTGGCTTG GGGCCAACTC CTTCCGCACT AGCCACTACC CCTATGCAGA GGAGATCATG 
GATCTGTGTG ACGCGTACGG CATCGTGGTG ATCGACGAGT GCCCAGCCGT GGGGATGCGA 
GATCAGGAGA ACTTTGGTAA TAAATCGCTG GCCCACCATC TCACGGTGAT GGAAGAGCTG 
ATCCGGCGGG ATAAGAACAG GCCCTCGGTT GTGATGTGGT CTGTAGCCAA TGAACCAGCC 
TCAGAGCTAA CCCCAGCAGC TTACTACTTT AAGACAGTGA TAGCTCACAC TAAAGCCATT 
GATCCCTCCA GACCTGTAAC CTTTGTGTCA AATGCTAATT ACGCTCATGA TCATGGTGCT 
CCATACGTGG ATGTAATTTG CGTAAATAGC TACTTCTCCT GGTACCACGA CTCCGGCCAT 
CTGGAGGTTA TTCAACTACA ACTCAATACA CAGTTTGAGA ACTGGTATGA AACCTACCAA 
AAGCCAATTA TCCAGAGTGA ATACGGAGCA GATTCTATTG CTGGGCTTCA CAATGACCCA 
CCTCTTATGT TCAGCGAGGA ATACCATAAG GCTGTGCTAC AGAAATACCA TTCAGTATTT 
GACAAAAAGA GAAAGGAATA TGTGATTGGG GAGCTCATCT GGAATTTTGC TGATTTTATG 
ACAGCGCAAG CAGTCATTCG TGTTTTAGGG AACAAGAAGG GGATCTTCAC CCGGCAACGA 
CAACCAAAGG CCGCTGCATT TATTCTAAGA GACAGATACT GGAAACTTGC AAATGAATCA 
AGTTGTATCC ACCCAACAGT GAAATATTAC AATTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006113175.1
CDS	1..1779
Translation	MRPHANRFTLCLPCSCQSGPVIDMPVPASFNELTQDPNLRQYVGWVWYEREVLLPRSWLLNALTTRVVLRVGSAHYYTIVWVNGVQVTEHEGGHLPFEADISRIVQAGPGALCRITVALNNTLTPHTLPPGAIQFLADESKYPKGYFVQNTKFDFFNYAGIHRPVVLYTTPTVYIDDITVTTAWTGNIGVVRYQVLVAGSTHYSLSLSLREKEGKVVATGNGTVGELKVVDPKLWWPYLMHENPGYLYYLEVTLLAQAGGESLEDLYTLPVGIRTVHVTRSQFLINGKPFYFHGVNKHEDADIRGKGLDWSLIVKDFNLLRWLGANSFRTSHYPYAEEIMDLCDAYGIVVIDECPAVGMRDQENFGNKSLAHHLTVMEELIRRDKNRPSVVMWSVANEPASELTPAAYYFKTVIAHTKAIDPSRPVTFVSNANYAHDHGAPYVDVICVNSYFSWYHDSGHLEVIQLQLNTQFENWYETYQKPIIQSEYGADSIAGLHNDPPLMFSEEYHKAVLQKYHSVFDKKRKEYVIGELIWNFADFMTAQAVIRVLGNKKGIFTRQRQPKAAAFILRDRYWKLANESSCIHPTVKYYNL

Target ORF information:

RefSeq Version	XM_006113113.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis glucuronidase, beta (GUSB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006113113.2

ORF Insert Sequence:

ATGCGTCCCC ACGCAAACCG TTTCACCCTC TGTCTCCCTT GCTCCTGCCA GAGTGGGCCT 
GTGATCGACA TGCCGGTACC GGCCAGCTTC AACGAGCTGA CTCAGGATCC CAATCTGCGG 
CAATATGTTG GCTGGGTTTG GTATGAGAGA GAGGTGCTGC TTCCCCGGAG CTGGCTGCTG 
AATGCCCTCA CCACCCGAGT CGTGCTGCGG GTGGGCAGTG CACATTACTA CACCATAGTC 
TGGGTCAATG GAGTGCAGGT GACGGAGCAT GAAGGGGGCC ACCTGCCCTT CGAAGCCGAC 
ATAAGCAGGA TTGTGCAGGC TGGCCCCGGG GCCCTGTGCC GCATCACCGT TGCTCTCAAC 
AACACCCTGA CGCCTCACAC CCTGCCTCCC GGCGCCATTC AGTTCCTGGC GGACGAGTCT 
AAGTATCCCA AGGGCTATTT TGTTCAGAAC ACAAAGTTTG ATTTCTTTAA CTACGCTGGA 
ATTCACCGTC CGGTCGTGCT GTATACAACC CCCACGGTCT ACATAGATGA CATCACTGTG 
ACTACAGCCT GGACAGGGAA TATTGGTGTG GTGCGGTATC AGGTGTTGGT GGCCGGCAGC 
ACCCACTACT CCTTGTCGCT GTCTCTCCGT GAGAAAGAGG GGAAAGTAGT TGCTACGGGA 
AATGGAACAG TCGGAGAGTT GAAAGTAGTG GATCCAAAGC TTTGGTGGCC TTATCTGATG 
CACGAAAACC CTGGATACCT TTATTACTTG GAGGTGACGC TGTTGGCGCA GGCGGGCGGG 
GAGTCGCTGG AAGATTTGTA CACGCTCCCC GTCGGGATCC GCACCGTACA TGTCACCCGC 
AGCCAGTTCC TCATCAACGG AAAGCCCTTC TACTTCCATG GCGTCAACAA GCACGAGGAT 
GCTGACATCC GCGGCAAAGG CCTGGACTGG TCACTGATTG TGAAGGATTT CAACCTACTG 
CGGTGGCTTG GGGCCAACTC CTTCCGCACT AGCCACTACC CCTATGCAGA GGAGATCATG 
GATCTGTGTG ACGCGTACGG CATCGTGGTG ATCGACGAGT GCCCAGCCGT GGGGATGCGA 
GATCAGGAGA ACTTTGGTAA TAAATCGCTG GCCCACCATC TCACGGTGAT GGAAGAGCTG 
ATCCGGCGGG ATAAGAACAG GCCCTCGGTT GTGATGTGGT CTGTAGCCAA TGAACCAGCC 
TCAGAGCTAA CCCCAGCAGC TTACTACTTT AAGACAGTGA TAGCTCACAC TAAAGCCATT 
GATCCCTCCA GACCTGTAAC CTTTGTGTCA AATGCTAATT ACGCTCATGA TCATGGTGCT 
CCATACGTGG ATGTAATTTG CGTAAATAGC TACTTCTCCT GGTACCACGA CTCCGGCCAT 
CTGGAGGTTA TTCAACTACA ACTCAATACA CAGTTTGAGA ACTGGTATGA AACCTACCAA 
AAGCCAATTA TCCAGAGTGA ATACGGAGCA GATTCTATTG CTGGGCTTCA CAATGACCCA 
CCTCTTATGT TCAGCGAGGA ATACCATAAG GCTGTGCTAC AGAAATACCA TTCAGTATTT 
GACAAAAAGA GAAAGGAATA TGTGATTGGG GAGCTCATCT GGAATTTTGC TGATTTTATG 
ACAGCGCAAG CAGTCATTCG TGTTTTAGGG AACAAGAAGG GGATCTTCAC CCGGCAACGA 
CAACCAAAGG CCGCTGCATT TATTCTAAGA GACAGATACT GGAAACTTGC AAATGAATCA 
AGTTGTATCC ACCCAACAGT GAAATATTAC AATTTGTGA