PDE6B cDNA ORF clone, Parus major(Great Tit)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE6B
Entrez Gene ID	107216334
Full Name	phosphodiesterase 6B
Gene Type	protein-coding
Organism	Parus major(Great Tit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015653327.2	XP_015508813.1	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
XM_015653329.2	XP_015508815.1	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta

Pathways from BioSystems
Gene Ontology

The following PDE6B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE6B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa185722	XM_015653329.2 Latest version!	Parus major phosphodiesterase 6B (PDE6B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPa185722	XM_015653327.2 Latest version!	Parus major phosphodiesterase 6B (PDE6B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE6B ( XM_015653327.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015508813.1 Parus major phosphodiesterase 6B (PDE6B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa185722
Clone ID Related Accession (Same CDS sequence)	XM_015653327.2 , XM_015653329.2
Accession Version	XM_015653327.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031799.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015653327.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTA GTGAAGACGA TGTGGAGAAG TTTCTTGATG GCAACCCTGC TTTTGCCAAA 
CAGTATTTTG AGAAGAAACT AAAAGCAGAG TCCTGGGATA ACAATGAGAG TGAAATACTG 
TTTGAATTGA TCCAAGACAT GCAAGAAAGC ATCAACATGG AGAAAGTTGT TTTCAAGACC 
TTGAGAAGAA TCAGGTCCCT TATTCACGCT GACCGCTGCA GTCTCTTCAT GTACAGGCAG 
AGAAATGGCA CTCCTGAACT GGCGACAAGG CTTTTCAACA TCCAAGAAGG AAGTACTCTG 
GAGGAATGCC TGGTCTCCCC AGACTGTGAG ATTGTCTATC CACTGGACAT AGGCATTGTG 
GGCCATGTTG CTCAGACCAA GAAAACCATG AACATCAAGG ATGTCCGTGA GTGTGCCCAG 
TTCAGCCCAT TTGTTGATGA GCTCACTGAC TACACTACAA AAAACATCCT TGCAACACCC 
ATCTTGAATG GCAAAGACTT GGTTGCTGTC ATTGTGGCTA TAAATAAGCT GAATGGCCCA 
CACTTCACCA GCTCTGATGA AACACTTTTT CTGAAGTATC TGAATTTTGC ATCCTTGAAC 
TTGAAAATTT ATCACTTGAG TTATCTTCAC AACTGTGAGA CTCGAAGAGG CCAGGTGCTG 
TTGTGGTCAG CTAATAAGGT TTTTGAGGAG CTGACGGACA TCGAGAGGCA GTTCCACAAG 
GCTTTTTATA CAGTGAGGGC ATACCTGAAC TGTGACCGAT ACTCGGTCGG CCTCCTGGAC 
ATGACAAAGC AGAAGGAATT TTTTGACCTG TGGCCAGTCC TGTTGGGAGA AGTACCTCCC 
TACTCAGGAC CTCGAACCCC AGATGGCAGG GAAATAGTCT TTTACAAGGT CATTGATTAT 
ATATTACATG GAAAAGAAGA CATTAAAGTC ATCCCAAATC CTACCCCGGA TCACTGGGCA 
CTAGTTACTG GACTGCCAGC CTATGTAGCT GAAAGTGGAT TTATTTGCAA CATTATGAAT 
GCTGCTGCAG ATGAGATGTT TAACTTTCAG GAAGGTCCTC TAGATGAATC AGGATGGACT 
ATCAAAAATG TTCTCTCCAT GCCAATAGTG AATAAAAGGG AAGAAATTGT TGGTGTTGTC 
ACATTTTATA ACAGAAAAGA TGGGAAACCA TTTGATGAGC AGGATGAAAC CCTCATGGAG 
TCCTTGACAC AGTTCCTGGG TTGGTCTGTA CTCAACACAG ACACATATGA TAAGATGAAC 
AAGCTGGAGA ACCGTAAGGA CATTGCTCAG GACATGGTGC TCTACCACGT GAAATGTGAC 
AAGGATGAAA TCCAGGAAAT TCTGCCGACG CGAGAGAAAC TGGGGAAGGA GCCAAGTGAG 
TGTGACGAAG AGGAGCTGGC AAGCATTCTG AAAGAAGAAC TCCCGGGACC CACTAAGTTC 
GAAATCTATG AGTTCAGGTT CTCTGATTTT GACTGCACTG AGCTAGAGCT GGTGAAGTGT 
GGCATTCAGA TGTACTACGA GCTTGGCGTG GTGAAAAAGT TCCAGATCCC ACAGGAGGTT 
CTGGTAAGGT TTGTGTACTC TGTCAGCAAA GGCTACCGGA AGATAACTTA CCACAACTGG 
CGGCACGGCT TCAATGTTGC ACAGACCATG TTCACGCTCC TGATGACTGG CAAACTGAAG 
CGTTACTACA CTGACCTTGA AGCCCTTGCA ATGGTAACTG CTGCTCTGTG CCATGATATT 
GACCACAGGG GAACCAACAA CCTCTACCAA ATGAAATCTC AAAATCCTTT AGCTAAACTT 
CATGGATCCT CTATTTTAGA GAGACATCAC CTGGAATTCG GAAAATTCTT GCTCTCTGAG 
GAGTCACTGA ATATATGTCA GAACCTGAAC CGCAGACAGC ATGAGCATGT GATTCACCTG 
ATGGAAATTG CCATTATAGC AACAGACCTG GCACTCTACT TCAAAAAGAG AACAATGTTT 
CAAAAGATCG TTGATGAGTC TAAGACATAC GACAGCGTGA CTGCCTGGAC TGAGTATCTG 
TCTCTGGAGA CAACAAAGAA GGAGGTTGTC ATGGCCATGA TGATGACTGC CTGTGACTTG 
TCAGCTATCA CAAAGCCTTG GGAAGTCCAG AGTAAGGTAG CTCTACTGGT AGCAGCTGAG 
TTCTGGGAGC AAGGAGATTT GGAAATAAGC GTCCTTCAGC AACAGCCCAT TCCTATGATG 
GACCGAAGGA AAGCTGCTGA GCTTCCAAAG CTTCAAGTGG GTTTCATCGA CTTTGTGTGC 
ACATTTGTCT ATAAGGAATT TTCCCGTTTC CATGAGGAAA TACAGCCTAT GCTTGATGGG 
CTGCTGAACA ACAGAAATGA GTGGAAGACT CGTGCTGATG AGTATGATGC AAAAATGAAA 
GCTCTAGAGG AAGAAAAGAA AAAAGAGGAA GAGAGAATGG CTGCAAAGAG AGGTGAAATA 
ACCTGTAATG GAGGAACAGC TCCAGCTTCC AAAACCTGTA GCATACTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015508813.1
CDS	4253..6763
Translation	MSISEDDVEKFLDGNPAFAKQYFEKKLKAESWDNNESEILFELIQDMQESINMEKVVFKTLRRIRSLIHADRCSLFMYRQRNGTPELATRLFNIQEGSTLEECLVSPDCEIVYPLDIGIVGHVAQTKKTMNIKDVRECAQFSPFVDELTDYTTKNILATPILNGKDLVAVIVAINKLNGPHFTSSDETLFLKYLNFASLNLKIYHLSYLHNCETRRGQVLLWSANKVFEELTDIERQFHKAFYTVRAYLNCDRYSVGLLDMTKQKEFFDLWPVLLGEVPPYSGPRTPDGREIVFYKVIDYILHGKEDIKVIPNPTPDHWALVTGLPAYVAESGFICNIMNAAADEMFNFQEGPLDESGWTIKNVLSMPIVNKREEIVGVVTFYNRKDGKPFDEQDETLMESLTQFLGWSVLNTDTYDKMNKLENRKDIAQDMVLYHVKCDKDEIQEILPTREKLGKEPSECDEEELASILKEELPGPTKFEIYEFRFSDFDCTELELVKCGIQMYYELGVVKKFQIPQEVLVRFVYSVSKGYRKITYHNWRHGFNVAQTMFTLLMTGKLKRYYTDLEALAMVTAALCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKFLLSEESLNICQNLNRRQHEHVIHLMEIAIIATDLALYFKKRTMFQKIVDESKTYDSVTAWTEYLSLETTKKEVVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLEISVLQQQPIPMMDRRKAAELPKLQVGFIDFVCTFVYKEFSRFHEEIQPMLDGLLNNRNEWKTRADEYDAKMKALEEEKKKEEERMAAKRGEITCNGGTAPASKTCSIL

Target ORF information:

RefSeq Version	XM_015653327.2
Organism	Parus major(Great Tit)
Definition	Parus major phosphodiesterase 6B (PDE6B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015653327.2

ORF Insert Sequence:

ATGAGCATTA GTGAAGACGA TGTGGAGAAG TTTCTTGATG GCAACCCTGC TTTTGCCAAA 
CAGTATTTTG AGAAGAAACT AAAAGCAGAG TCCTGGGATA ACAATGAGAG TGAAATACTG 
TTTGAATTGA TCCAAGACAT GCAAGAAAGC ATCAACATGG AGAAAGTTGT TTTCAAGACC 
TTGAGAAGAA TCAGGTCCCT TATTCACGCT GACCGCTGCA GTCTCTTCAT GTACAGGCAG 
AGAAATGGCA CTCCTGAACT GGCGACAAGG CTTTTCAACA TCCAAGAAGG AAGTACTCTG 
GAGGAATGCC TGGTCTCCCC AGACTGTGAG ATTGTCTATC CACTGGACAT AGGCATTGTG 
GGCCATGTTG CTCAGACCAA GAAAACCATG AACATCAAGG ATGTCCGTGA GTGTGCCCAG 
TTCAGCCCAT TTGTTGATGA GCTCACTGAC TACACTACAA AAAACATCCT TGCAACACCC 
ATCTTGAATG GCAAAGACTT GGTTGCTGTC ATTGTGGCTA TAAATAAGCT GAATGGCCCA 
CACTTCACCA GCTCTGATGA AACACTTTTT CTGAAGTATC TGAATTTTGC ATCCTTGAAC 
TTGAAAATTT ATCACTTGAG TTATCTTCAC AACTGTGAGA CTCGAAGAGG CCAGGTGCTG 
TTGTGGTCAG CTAATAAGGT TTTTGAGGAG CTGACGGACA TCGAGAGGCA GTTCCACAAG 
GCTTTTTATA CAGTGAGGGC ATACCTGAAC TGTGACCGAT ACTCGGTCGG CCTCCTGGAC 
ATGACAAAGC AGAAGGAATT TTTTGACCTG TGGCCAGTCC TGTTGGGAGA AGTACCTCCC 
TACTCAGGAC CTCGAACCCC AGATGGCAGG GAAATAGTCT TTTACAAGGT CATTGATTAT 
ATATTACATG GAAAAGAAGA CATTAAAGTC ATCCCAAATC CTACCCCGGA TCACTGGGCA 
CTAGTTACTG GACTGCCAGC CTATGTAGCT GAAAGTGGAT TTATTTGCAA CATTATGAAT 
GCTGCTGCAG ATGAGATGTT TAACTTTCAG GAAGGTCCTC TAGATGAATC AGGATGGACT 
ATCAAAAATG TTCTCTCCAT GCCAATAGTG AATAAAAGGG AAGAAATTGT TGGTGTTGTC 
ACATTTTATA ACAGAAAAGA TGGGAAACCA TTTGATGAGC AGGATGAAAC CCTCATGGAG 
TCCTTGACAC AGTTCCTGGG TTGGTCTGTA CTCAACACAG ACACATATGA TAAGATGAAC 
AAGCTGGAGA ACCGTAAGGA CATTGCTCAG GACATGGTGC TCTACCACGT GAAATGTGAC 
AAGGATGAAA TCCAGGAAAT TCTGCCGACG CGAGAGAAAC TGGGGAAGGA GCCAAGTGAG 
TGTGACGAAG AGGAGCTGGC AAGCATTCTG AAAGAAGAAC TCCCGGGACC CACTAAGTTC 
GAAATCTATG AGTTCAGGTT CTCTGATTTT GACTGCACTG AGCTAGAGCT GGTGAAGTGT 
GGCATTCAGA TGTACTACGA GCTTGGCGTG GTGAAAAAGT TCCAGATCCC ACAGGAGGTT 
CTGGTAAGGT TTGTGTACTC TGTCAGCAAA GGCTACCGGA AGATAACTTA CCACAACTGG 
CGGCACGGCT TCAATGTTGC ACAGACCATG TTCACGCTCC TGATGACTGG CAAACTGAAG 
CGTTACTACA CTGACCTTGA AGCCCTTGCA ATGGTAACTG CTGCTCTGTG CCATGATATT 
GACCACAGGG GAACCAACAA CCTCTACCAA ATGAAATCTC AAAATCCTTT AGCTAAACTT 
CATGGATCCT CTATTTTAGA GAGACATCAC CTGGAATTCG GAAAATTCTT GCTCTCTGAG 
GAGTCACTGA ATATATGTCA GAACCTGAAC CGCAGACAGC ATGAGCATGT GATTCACCTG 
ATGGAAATTG CCATTATAGC AACAGACCTG GCACTCTACT TCAAAAAGAG AACAATGTTT 
CAAAAGATCG TTGATGAGTC TAAGACATAC GACAGCGTGA CTGCCTGGAC TGAGTATCTG 
TCTCTGGAGA CAACAAAGAA GGAGGTTGTC ATGGCCATGA TGATGACTGC CTGTGACTTG 
TCAGCTATCA CAAAGCCTTG GGAAGTCCAG AGTAAGGTAG CTCTACTGGT AGCAGCTGAG 
TTCTGGGAGC AAGGAGATTT GGAAATAAGC GTCCTTCAGC AACAGCCCAT TCCTATGATG 
GACCGAAGGA AAGCTGCTGA GCTTCCAAAG CTTCAAGTGG GTTTCATCGA CTTTGTGTGC 
ACATTTGTCT ATAAGGAATT TTCCCGTTTC CATGAGGAAA TACAGCCTAT GCTTGATGGG 
CTGCTGAACA ACAGAAATGA GTGGAAGACT CGTGCTGATG AGTATGATGC AAAAATGAAA 
GCTCTAGAGG AAGAAAAGAA AAAAGAGGAA GAGAGAATGG CTGCAAAGAG AGGTGAAATA 
ACCTGTAATG GAGGAACAGC TCCAGCTTCC AAAACCTGTA GCATACTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE6B ( XM_015653329.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015508815.1 Parus major phosphodiesterase 6B (PDE6B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa185722
Clone ID Related Accession (Same CDS sequence)	XM_015653327.2 , XM_015653329.2
Accession Version	XM_015653329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031799.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015653329.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTA GTGAAGACGA TGTGGAGAAG TTTCTTGATG GCAACCCTGC TTTTGCCAAA 
CAGTATTTTG AGAAGAAACT AAAAGCAGAG TCCTGGGATA ACAATGAGAG TGAAATACTG 
TTTGAATTGA TCCAAGACAT GCAAGAAAGC ATCAACATGG AGAAAGTTGT TTTCAAGACC 
TTGAGAAGAA TCAGGTCCCT TATTCACGCT GACCGCTGCA GTCTCTTCAT GTACAGGCAG 
AGAAATGGCA CTCCTGAACT GGCGACAAGG CTTTTCAACA TCCAAGAAGG AAGTACTCTG 
GAGGAATGCC TGGTCTCCCC AGACTGTGAG ATTGTCTATC CACTGGACAT AGGCATTGTG 
GGCCATGTTG CTCAGACCAA GAAAACCATG AACATCAAGG ATGTCCGTGA GTGTGCCCAG 
TTCAGCCCAT TTGTTGATGA GCTCACTGAC TACACTACAA AAAACATCCT TGCAACACCC 
ATCTTGAATG GCAAAGACTT GGTTGCTGTC ATTGTGGCTA TAAATAAGCT GAATGGCCCA 
CACTTCACCA GCTCTGATGA AACACTTTTT CTGAAGTATC TGAATTTTGC ATCCTTGAAC 
TTGAAAATTT ATCACTTGAG TTATCTTCAC AACTGTGAGA CTCGAAGAGG CCAGGTGCTG 
TTGTGGTCAG CTAATAAGGT TTTTGAGGAG CTGACGGACA TCGAGAGGCA GTTCCACAAG 
GCTTTTTATA CAGTGAGGGC ATACCTGAAC TGTGACCGAT ACTCGGTCGG CCTCCTGGAC 
ATGACAAAGC AGAAGGAATT TTTTGACCTG TGGCCAGTCC TGTTGGGAGA AGTACCTCCC 
TACTCAGGAC CTCGAACCCC AGATGGCAGG GAAATAGTCT TTTACAAGGT CATTGATTAT 
ATATTACATG GAAAAGAAGA CATTAAAGTC ATCCCAAATC CTACCCCGGA TCACTGGGCA 
CTAGTTACTG GACTGCCAGC CTATGTAGCT GAAAGTGGAT TTATTTGCAA CATTATGAAT 
GCTGCTGCAG ATGAGATGTT TAACTTTCAG GAAGGTCCTC TAGATGAATC AGGATGGACT 
ATCAAAAATG TTCTCTCCAT GCCAATAGTG AATAAAAGGG AAGAAATTGT TGGTGTTGTC 
ACATTTTATA ACAGAAAAGA TGGGAAACCA TTTGATGAGC AGGATGAAAC CCTCATGGAG 
TCCTTGACAC AGTTCCTGGG TTGGTCTGTA CTCAACACAG ACACATATGA TAAGATGAAC 
AAGCTGGAGA ACCGTAAGGA CATTGCTCAG GACATGGTGC TCTACCACGT GAAATGTGAC 
AAGGATGAAA TCCAGGAAAT TCTGCCGACG CGAGAGAAAC TGGGGAAGGA GCCAAGTGAG 
TGTGACGAAG AGGAGCTGGC AAGCATTCTG AAAGAAGAAC TCCCGGGACC CACTAAGTTC 
GAAATCTATG AGTTCAGGTT CTCTGATTTT GACTGCACTG AGCTAGAGCT GGTGAAGTGT 
GGCATTCAGA TGTACTACGA GCTTGGCGTG GTGAAAAAGT TCCAGATCCC ACAGGAGGTT 
CTGGTAAGGT TTGTGTACTC TGTCAGCAAA GGCTACCGGA AGATAACTTA CCACAACTGG 
CGGCACGGCT TCAATGTTGC ACAGACCATG TTCACGCTCC TGATGACTGG CAAACTGAAG 
CGTTACTACA CTGACCTTGA AGCCCTTGCA ATGGTAACTG CTGCTCTGTG CCATGATATT 
GACCACAGGG GAACCAACAA CCTCTACCAA ATGAAATCTC AAAATCCTTT AGCTAAACTT 
CATGGATCCT CTATTTTAGA GAGACATCAC CTGGAATTCG GAAAATTCTT GCTCTCTGAG 
GAGTCACTGA ATATATGTCA GAACCTGAAC CGCAGACAGC ATGAGCATGT GATTCACCTG 
ATGGAAATTG CCATTATAGC AACAGACCTG GCACTCTACT TCAAAAAGAG AACAATGTTT 
CAAAAGATCG TTGATGAGTC TAAGACATAC GACAGCGTGA CTGCCTGGAC TGAGTATCTG 
TCTCTGGAGA CAACAAAGAA GGAGGTTGTC ATGGCCATGA TGATGACTGC CTGTGACTTG 
TCAGCTATCA CAAAGCCTTG GGAAGTCCAG AGTAAGGTAG CTCTACTGGT AGCAGCTGAG 
TTCTGGGAGC AAGGAGATTT GGAAATAAGC GTCCTTCAGC AACAGCCCAT TCCTATGATG 
GACCGAAGGA AAGCTGCTGA GCTTCCAAAG CTTCAAGTGG GTTTCATCGA CTTTGTGTGC 
ACATTTGTCT ATAAGGAATT TTCCCGTTTC CATGAGGAAA TACAGCCTAT GCTTGATGGG 
CTGCTGAACA ACAGAAATGA GTGGAAGACT CGTGCTGATG AGTATGATGC AAAAATGAAA 
GCTCTAGAGG AAGAAAAGAA AAAAGAGGAA GAGAGAATGG CTGCAAAGAG AGGTGAAATA 
ACCTGTAATG GAGGAACAGC TCCAGCTTCC AAAACCTGTA GCATACTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015508815.1
CDS	3587..6097
Translation	MSISEDDVEKFLDGNPAFAKQYFEKKLKAESWDNNESEILFELIQDMQESINMEKVVFKTLRRIRSLIHADRCSLFMYRQRNGTPELATRLFNIQEGSTLEECLVSPDCEIVYPLDIGIVGHVAQTKKTMNIKDVRECAQFSPFVDELTDYTTKNILATPILNGKDLVAVIVAINKLNGPHFTSSDETLFLKYLNFASLNLKIYHLSYLHNCETRRGQVLLWSANKVFEELTDIERQFHKAFYTVRAYLNCDRYSVGLLDMTKQKEFFDLWPVLLGEVPPYSGPRTPDGREIVFYKVIDYILHGKEDIKVIPNPTPDHWALVTGLPAYVAESGFICNIMNAAADEMFNFQEGPLDESGWTIKNVLSMPIVNKREEIVGVVTFYNRKDGKPFDEQDETLMESLTQFLGWSVLNTDTYDKMNKLENRKDIAQDMVLYHVKCDKDEIQEILPTREKLGKEPSECDEEELASILKEELPGPTKFEIYEFRFSDFDCTELELVKCGIQMYYELGVVKKFQIPQEVLVRFVYSVSKGYRKITYHNWRHGFNVAQTMFTLLMTGKLKRYYTDLEALAMVTAALCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKFLLSEESLNICQNLNRRQHEHVIHLMEIAIIATDLALYFKKRTMFQKIVDESKTYDSVTAWTEYLSLETTKKEVVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLEISVLQQQPIPMMDRRKAAELPKLQVGFIDFVCTFVYKEFSRFHEEIQPMLDGLLNNRNEWKTRADEYDAKMKALEEEKKKEEERMAAKRGEITCNGGTAPASKTCSIL

Target ORF information:

RefSeq Version	XM_015653329.2
Organism	Parus major(Great Tit)
Definition	Parus major phosphodiesterase 6B (PDE6B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015653329.2

ORF Insert Sequence:

ATGAGCATTA GTGAAGACGA TGTGGAGAAG TTTCTTGATG GCAACCCTGC TTTTGCCAAA 
CAGTATTTTG AGAAGAAACT AAAAGCAGAG TCCTGGGATA ACAATGAGAG TGAAATACTG 
TTTGAATTGA TCCAAGACAT GCAAGAAAGC ATCAACATGG AGAAAGTTGT TTTCAAGACC 
TTGAGAAGAA TCAGGTCCCT TATTCACGCT GACCGCTGCA GTCTCTTCAT GTACAGGCAG 
AGAAATGGCA CTCCTGAACT GGCGACAAGG CTTTTCAACA TCCAAGAAGG AAGTACTCTG 
GAGGAATGCC TGGTCTCCCC AGACTGTGAG ATTGTCTATC CACTGGACAT AGGCATTGTG 
GGCCATGTTG CTCAGACCAA GAAAACCATG AACATCAAGG ATGTCCGTGA GTGTGCCCAG 
TTCAGCCCAT TTGTTGATGA GCTCACTGAC TACACTACAA AAAACATCCT TGCAACACCC 
ATCTTGAATG GCAAAGACTT GGTTGCTGTC ATTGTGGCTA TAAATAAGCT GAATGGCCCA 
CACTTCACCA GCTCTGATGA AACACTTTTT CTGAAGTATC TGAATTTTGC ATCCTTGAAC 
TTGAAAATTT ATCACTTGAG TTATCTTCAC AACTGTGAGA CTCGAAGAGG CCAGGTGCTG 
TTGTGGTCAG CTAATAAGGT TTTTGAGGAG CTGACGGACA TCGAGAGGCA GTTCCACAAG 
GCTTTTTATA CAGTGAGGGC ATACCTGAAC TGTGACCGAT ACTCGGTCGG CCTCCTGGAC 
ATGACAAAGC AGAAGGAATT TTTTGACCTG TGGCCAGTCC TGTTGGGAGA AGTACCTCCC 
TACTCAGGAC CTCGAACCCC AGATGGCAGG GAAATAGTCT TTTACAAGGT CATTGATTAT 
ATATTACATG GAAAAGAAGA CATTAAAGTC ATCCCAAATC CTACCCCGGA TCACTGGGCA 
CTAGTTACTG GACTGCCAGC CTATGTAGCT GAAAGTGGAT TTATTTGCAA CATTATGAAT 
GCTGCTGCAG ATGAGATGTT TAACTTTCAG GAAGGTCCTC TAGATGAATC AGGATGGACT 
ATCAAAAATG TTCTCTCCAT GCCAATAGTG AATAAAAGGG AAGAAATTGT TGGTGTTGTC 
ACATTTTATA ACAGAAAAGA TGGGAAACCA TTTGATGAGC AGGATGAAAC CCTCATGGAG 
TCCTTGACAC AGTTCCTGGG TTGGTCTGTA CTCAACACAG ACACATATGA TAAGATGAAC 
AAGCTGGAGA ACCGTAAGGA CATTGCTCAG GACATGGTGC TCTACCACGT GAAATGTGAC 
AAGGATGAAA TCCAGGAAAT TCTGCCGACG CGAGAGAAAC TGGGGAAGGA GCCAAGTGAG 
TGTGACGAAG AGGAGCTGGC AAGCATTCTG AAAGAAGAAC TCCCGGGACC CACTAAGTTC 
GAAATCTATG AGTTCAGGTT CTCTGATTTT GACTGCACTG AGCTAGAGCT GGTGAAGTGT 
GGCATTCAGA TGTACTACGA GCTTGGCGTG GTGAAAAAGT TCCAGATCCC ACAGGAGGTT 
CTGGTAAGGT TTGTGTACTC TGTCAGCAAA GGCTACCGGA AGATAACTTA CCACAACTGG 
CGGCACGGCT TCAATGTTGC ACAGACCATG TTCACGCTCC TGATGACTGG CAAACTGAAG 
CGTTACTACA CTGACCTTGA AGCCCTTGCA ATGGTAACTG CTGCTCTGTG CCATGATATT 
GACCACAGGG GAACCAACAA CCTCTACCAA ATGAAATCTC AAAATCCTTT AGCTAAACTT 
CATGGATCCT CTATTTTAGA GAGACATCAC CTGGAATTCG GAAAATTCTT GCTCTCTGAG 
GAGTCACTGA ATATATGTCA GAACCTGAAC CGCAGACAGC ATGAGCATGT GATTCACCTG 
ATGGAAATTG CCATTATAGC AACAGACCTG GCACTCTACT TCAAAAAGAG AACAATGTTT 
CAAAAGATCG TTGATGAGTC TAAGACATAC GACAGCGTGA CTGCCTGGAC TGAGTATCTG 
TCTCTGGAGA CAACAAAGAA GGAGGTTGTC ATGGCCATGA TGATGACTGC CTGTGACTTG 
TCAGCTATCA CAAAGCCTTG GGAAGTCCAG AGTAAGGTAG CTCTACTGGT AGCAGCTGAG 
TTCTGGGAGC AAGGAGATTT GGAAATAAGC GTCCTTCAGC AACAGCCCAT TCCTATGATG 
GACCGAAGGA AAGCTGCTGA GCTTCCAAAG CTTCAAGTGG GTTTCATCGA CTTTGTGTGC 
ACATTTGTCT ATAAGGAATT TTCCCGTTTC CATGAGGAAA TACAGCCTAT GCTTGATGGG 
CTGCTGAACA ACAGAAATGA GTGGAAGACT CGTGCTGATG AGTATGATGC AAAAATGAAA 
GCTCTAGAGG AAGAAAAGAA AAAAGAGGAA GAGAGAATGG CTGCAAAGAG AGGTGAAATA 
ACCTGTAATG GAGGAACAGC TCCAGCTTCC AAAACCTGTA GCATACTTTA G