smpdl3b cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene
Clones
gRNAs

Summary

Gene Symbol	smpdl3b
Entrez Gene ID	109633132
Full Name	sphingomyelin phosphodiesterase acid like 3B
Gene Type	protein-coding
Organism	Paralichthys olivaceus(Japanese flounder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020092752.1	XP_019948311.1	acid sphingomyelinase-like phosphodiesterase 3b isoform X1
XM_020092753.1	XP_019948312.1	acid sphingomyelinase-like phosphodiesterase 3b isoform X2

Pathways from BioSystems
Gene Ontology

The following smpdl3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smpdl3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa244892	XM_020092752.1 Latest version!	Paralichthys olivaceus sphingomyelin phosphodiesterase acid like 3B (smpdl3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OPa244893	XM_020092753.1 Latest version!	Paralichthys olivaceus sphingomyelin phosphodiesterase acid like 3B (smpdl3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smpdl3b ( XM_020092752.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019948311.1 Paralichthys olivaceus sphingomyelin phosphodiesterase acid like 3B (smpdl3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa244892
Clone ID Related Accession (Same CDS sequence)	XM_020092752.1
Accession Version	XM_020092752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	acid sphingomyelinase-like phosphodiesterase 3b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859661.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGTTT TCTTCAAAGA GCTCAACATG TCGGTGGTGA AGCTGATCCT GTCTTATCTG 
CTGTTCAAAG AGGCTCATGC GCTGTCAGGG AACTTCTGGC ACATCACAGA TCTGCATTGG 
GACTACACGT TCAACCTGAG CAATGACCCT GAACTTGTGT GCAACTCAAG CAGCAATCGA 
CCCGCAACCA ATGCTGGGAA GTATGGAGAC TATGTTTGTG ACTCACCACT GCATCTTATC 
AACTCCTCTG TGTATGCCAT GAAGGATATT CTACCAGACC CGGACTTCAT CGTGTGGACA 
GGAGATAACA CACCACATGT ACCCAATAAA TACCTGGATG AAAAGAAAGT GCTGCACATT 
ATCAGCCGCC TCACTCACAT TATAAAACAG GTCTTTCCAC ACACTAAAGT GTACTCCGCC 
CTGGGAAACC ATGACTACCA CCAAAAGAAC CAGCTTCCCA CATTCTCTAA CAACATCTAT 
AACCAGACAG CAGAAATGTG GCAGGACTGG TTGGATCCTG AGTCCCAAGA AACATTTAGA 
AAAGGTGGAT ATTACACAGA AAGGTTGCTG AATCGAACAG GGTTCAGGAT GCTGGTCCTC 
AACACTAACC TCTATTATGA CCAAAACAAG GAGACCGTGG GTATGGAAGA TCCAGCGGAC 
CAGTTCAATT GGGCTGATCG TATTCTTACA GAGGCCGCCA ACAACAAAGA GAAGGTTTAC 
ATCATTGGCC ACGTCCCCCC AGGTTTCTTT GAGAAGAAGA GATTTAAGCC GTGGTTCACG 
TCTGAATTCA ACCAGGTATA CTTGGAGTTA ATTCAGAGGC ATCATTCTGT CATCCTCGGG 
CAGTTCTTTG GCCATCACCA CACAGACAGT TTCCGCATGT TCTACAGCTC AGAGGAGCCA 
CGCTCTCCTA TCAGTACCAT GTTCCTCAGC CCAGGGGTCA CACCGTGGAA AACAACACTT 
CCTGGAGTCG AGGATGGCGC AAACAACCCT GGGATTCGTG TCTTTGAATA TGACACCCAA 
ACACTCCTGG TCAAAGATGT GGTGACTTAT TATCTGAACC TGACTCATGC TAATGGAGCA 
CGTGGGCGCT GGGAGAAAGA GTACCGCCTT ACAGAGAGCT TCAGAGTGCC GGACGCCTCC 
CCAGCTTCCA TGCACCAGGT CCTGGAGCGC ATAGCTAACA ACCGCTGCTA CCTGCAGAAG 
TACTACGAGT TCAACTCTGT CAGTTATGAC CTGACAGAGT GTGACAGCGA CTGCCGCATT 
GACCACGTTT GTGCAGCCAG AGAGGTGGAC TTTGAGAAGT ACGAATGCTG CCTGCAAAAA 
GAAGGTGCCG CTGCTATTCA GAGTGGGATT CTGCCACTCA TCTCTGTGGC TGTAAGTCTG 
GTGTTGGCCA GTCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019948311.1
CDS	8..1405
Translation	MLVFFKELNMSVVKLILSYLLFKEAHALSGNFWHITDLHWDYTFNLSNDPELVCNSSSNRPATNAGKYGDYVCDSPLHLINSSVYAMKDILPDPDFIVWTGDNTPHVPNKYLDEKKVLHIISRLTHIIKQVFPHTKVYSALGNHDYHQKNQLPTFSNNIYNQTAEMWQDWLDPESQETFRKGGYYTERLLNRTGFRMLVLNTNLYYDQNKETVGMEDPADQFNWADRILTEAANNKEKVYIIGHVPPGFFEKKRFKPWFTSEFNQVYLELIQRHHSVILGQFFGHHHTDSFRMFYSSEEPRSPISTMFLSPGVTPWKTTLPGVEDGANNPGIRVFEYDTQTLLVKDVVTYYLNLTHANGARGRWEKEYRLTESFRVPDASPASMHQVLERIANNRCYLQKYYEFNSVSYDLTECDSDCRIDHVCAAREVDFEKYECCLQKEGAAAIQSGILPLISVAVSLVLASR

Target ORF information:

RefSeq Version	XM_020092752.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus sphingomyelin phosphodiesterase acid like 3B (smpdl3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020092752.1

ORF Insert Sequence:

ATGCTTGTTT TCTTCAAAGA GCTCAACATG TCGGTGGTGA AGCTGATCCT GTCTTATCTG 
CTGTTCAAAG AGGCTCATGC GCTGTCAGGG AACTTCTGGC ACATCACAGA TCTGCATTGG 
GACTACACGT TCAACCTGAG CAATGACCCT GAACTTGTGT GCAACTCAAG CAGCAATCGA 
CCCGCAACCA ATGCTGGGAA GTATGGAGAC TATGTTTGTG ACTCACCACT GCATCTTATC 
AACTCCTCTG TGTATGCCAT GAAGGATATT CTACCAGACC CGGACTTCAT CGTGTGGACA 
GGAGATAACA CACCACATGT ACCCAATAAA TACCTGGATG AAAAGAAAGT GCTGCACATT 
ATCAGCCGCC TCACTCACAT TATAAAACAG GTCTTTCCAC ACACTAAAGT GTACTCCGCC 
CTGGGAAACC ATGACTACCA CCAAAAGAAC CAGCTTCCCA CATTCTCTAA CAACATCTAT 
AACCAGACAG CAGAAATGTG GCAGGACTGG TTGGATCCTG AGTCCCAAGA AACATTTAGA 
AAAGGTGGAT ATTACACAGA AAGGTTGCTG AATCGAACAG GGTTCAGGAT GCTGGTCCTC 
AACACTAACC TCTATTATGA CCAAAACAAG GAGACCGTGG GTATGGAAGA TCCAGCGGAC 
CAGTTCAATT GGGCTGATCG TATTCTTACA GAGGCCGCCA ACAACAAAGA GAAGGTTTAC 
ATCATTGGCC ACGTCCCCCC AGGTTTCTTT GAGAAGAAGA GATTTAAGCC GTGGTTCACG 
TCTGAATTCA ACCAGGTATA CTTGGAGTTA ATTCAGAGGC ATCATTCTGT CATCCTCGGG 
CAGTTCTTTG GCCATCACCA CACAGACAGT TTCCGCATGT TCTACAGCTC AGAGGAGCCA 
CGCTCTCCTA TCAGTACCAT GTTCCTCAGC CCAGGGGTCA CACCGTGGAA AACAACACTT 
CCTGGAGTCG AGGATGGCGC AAACAACCCT GGGATTCGTG TCTTTGAATA TGACACCCAA 
ACACTCCTGG TCAAAGATGT GGTGACTTAT TATCTGAACC TGACTCATGC TAATGGAGCA 
CGTGGGCGCT GGGAGAAAGA GTACCGCCTT ACAGAGAGCT TCAGAGTGCC GGACGCCTCC 
CCAGCTTCCA TGCACCAGGT CCTGGAGCGC ATAGCTAACA ACCGCTGCTA CCTGCAGAAG 
TACTACGAGT TCAACTCTGT CAGTTATGAC CTGACAGAGT GTGACAGCGA CTGCCGCATT 
GACCACGTTT GTGCAGCCAG AGAGGTGGAC TTTGAGAAGT ACGAATGCTG CCTGCAAAAA 
GAAGGTGCCG CTGCTATTCA GAGTGGGATT CTGCCACTCA TCTCTGTGGC TGTAAGTCTG 
GTGTTGGCCA GTCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smpdl3b ( XM_020092753.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019948312.1 Paralichthys olivaceus sphingomyelin phosphodiesterase acid like 3B (smpdl3b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa244893
Clone ID Related Accession (Same CDS sequence)	XM_020092753.1
Accession Version	XM_020092753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	acid sphingomyelinase-like phosphodiesterase 3b isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859661.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAT TTGAGCTCAA CATGTCGGTG GTGAAGCTGA TCCTGTCTTA TCTGCTGTTC 
AAAGAGGCTC ATGCGCTGTC AGGGAACTTC TGGCACATCA CAGATCTGCA TTGGGACTAC 
ACGTTCAACC TGAGCAATGA CCCTGAACTT GTGTGCAACT CAAGCAGCAA TCGACCCGCA 
ACCAATGCTG GGAAGTATGG AGACTATGTT TGTGACTCAC CACTGCATCT TATCAACTCC 
TCTGTGTATG CCATGAAGGA TATTCTACCA GACCCGGACT TCATCGTGTG GACAGGAGAT 
AACACACCAC ATGTACCCAA TAAATACCTG GATGAAAAGA AAGTGCTGCA CATTATCAGC 
CGCCTCACTC ACATTATAAA ACAGGTCTTT CCACACACTA AAGTGTACTC CGCCCTGGGA 
AACCATGACT ACCACCAAAA GAACCAGCTT CCCACATTCT CTAACAACAT CTATAACCAG 
ACAGCAGAAA TGTGGCAGGA CTGGTTGGAT CCTGAGTCCC AAGAAACATT TAGAAAAGGT 
GGATATTACA CAGAAAGGTT GCTGAATCGA ACAGGGTTCA GGATGCTGGT CCTCAACACT 
AACCTCTATT ATGACCAAAA CAAGGAGACC GTGGGTATGG AAGATCCAGC GGACCAGTTC 
AATTGGGCTG ATCGTATTCT TACAGAGGCC GCCAACAACA AAGAGAAGGT TTACATCATT 
GGCCACGTCC CCCCAGGTTT CTTTGAGAAG AAGAGATTTA AGCCGTGGTT CACGTCTGAA 
TTCAACCAGG TATACTTGGA GTTAATTCAG AGGCATCATT CTGTCATCCT CGGGCAGTTC 
TTTGGCCATC ACCACACAGA CAGTTTCCGC ATGTTCTACA GCTCAGAGGA GCCACGCTCT 
CCTATCAGTA CCATGTTCCT CAGCCCAGGG GTCACACCGT GGAAAACAAC ACTTCCTGGA 
GTCGAGGATG GCGCAAACAA CCCTGGGATT CGTGTCTTTG AATATGACAC CCAAACACTC 
CTGGTCAAAG ATGTGGTGAC TTATTATCTG AACCTGACTC ATGCTAATGG AGCACGTGGG 
CGCTGGGAGA AAGAGTACCG CCTTACAGAG AGCTTCAGAG TGCCGGACGC CTCCCCAGCT 
TCCATGCACC AGGTCCTGGA GCGCATAGCT AACAACCGCT GCTACCTGCA GAAGTACTAC 
GAGTTCAACT CTGTCAGTTA TGACCTGACA GAGTGTGACA GCGACTGCCG CATTGACCAC 
GTTTGTGCAG CCAGAGAGGT GGACTTTGAG AAGTACGAAT GCTGCCTGCA AAAAGAAGGT 
GCCGCTGCTA TTCAGAGTGG GATTCTGCCA CTCATCTCTG TGGCTGTAAG TCTGGTGTTG 
GCCAGTCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019948312.1
CDS	60..1451
Translation	MEGFELNMSVVKLILSYLLFKEAHALSGNFWHITDLHWDYTFNLSNDPELVCNSSSNRPATNAGKYGDYVCDSPLHLINSSVYAMKDILPDPDFIVWTGDNTPHVPNKYLDEKKVLHIISRLTHIIKQVFPHTKVYSALGNHDYHQKNQLPTFSNNIYNQTAEMWQDWLDPESQETFRKGGYYTERLLNRTGFRMLVLNTNLYYDQNKETVGMEDPADQFNWADRILTEAANNKEKVYIIGHVPPGFFEKKRFKPWFTSEFNQVYLELIQRHHSVILGQFFGHHHTDSFRMFYSSEEPRSPISTMFLSPGVTPWKTTLPGVEDGANNPGIRVFEYDTQTLLVKDVVTYYLNLTHANGARGRWEKEYRLTESFRVPDASPASMHQVLERIANNRCYLQKYYEFNSVSYDLTECDSDCRIDHVCAAREVDFEKYECCLQKEGAAAIQSGILPLISVAVSLVLASR

Target ORF information:

RefSeq Version	XM_020092753.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus sphingomyelin phosphodiesterase acid like 3B (smpdl3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020092753.1

ORF Insert Sequence:

ATGGAGGGAT TTGAGCTCAA CATGTCGGTG GTGAAGCTGA TCCTGTCTTA TCTGCTGTTC 
AAAGAGGCTC ATGCGCTGTC AGGGAACTTC TGGCACATCA CAGATCTGCA TTGGGACTAC 
ACGTTCAACC TGAGCAATGA CCCTGAACTT GTGTGCAACT CAAGCAGCAA TCGACCCGCA 
ACCAATGCTG GGAAGTATGG AGACTATGTT TGTGACTCAC CACTGCATCT TATCAACTCC 
TCTGTGTATG CCATGAAGGA TATTCTACCA GACCCGGACT TCATCGTGTG GACAGGAGAT 
AACACACCAC ATGTACCCAA TAAATACCTG GATGAAAAGA AAGTGCTGCA CATTATCAGC 
CGCCTCACTC ACATTATAAA ACAGGTCTTT CCACACACTA AAGTGTACTC CGCCCTGGGA 
AACCATGACT ACCACCAAAA GAACCAGCTT CCCACATTCT CTAACAACAT CTATAACCAG 
ACAGCAGAAA TGTGGCAGGA CTGGTTGGAT CCTGAGTCCC AAGAAACATT TAGAAAAGGT 
GGATATTACA CAGAAAGGTT GCTGAATCGA ACAGGGTTCA GGATGCTGGT CCTCAACACT 
AACCTCTATT ATGACCAAAA CAAGGAGACC GTGGGTATGG AAGATCCAGC GGACCAGTTC 
AATTGGGCTG ATCGTATTCT TACAGAGGCC GCCAACAACA AAGAGAAGGT TTACATCATT 
GGCCACGTCC CCCCAGGTTT CTTTGAGAAG AAGAGATTTA AGCCGTGGTT CACGTCTGAA 
TTCAACCAGG TATACTTGGA GTTAATTCAG AGGCATCATT CTGTCATCCT CGGGCAGTTC 
TTTGGCCATC ACCACACAGA CAGTTTCCGC ATGTTCTACA GCTCAGAGGA GCCACGCTCT 
CCTATCAGTA CCATGTTCCT CAGCCCAGGG GTCACACCGT GGAAAACAAC ACTTCCTGGA 
GTCGAGGATG GCGCAAACAA CCCTGGGATT CGTGTCTTTG AATATGACAC CCAAACACTC 
CTGGTCAAAG ATGTGGTGAC TTATTATCTG AACCTGACTC ATGCTAATGG AGCACGTGGG 
CGCTGGGAGA AAGAGTACCG CCTTACAGAG AGCTTCAGAG TGCCGGACGC CTCCCCAGCT 
TCCATGCACC AGGTCCTGGA GCGCATAGCT AACAACCGCT GCTACCTGCA GAAGTACTAC 
GAGTTCAACT CTGTCAGTTA TGACCTGACA GAGTGTGACA GCGACTGCCG CATTGACCAC 
GTTTGTGCAG CCAGAGAGGT GGACTTTGAG AAGTACGAAT GCTGCCTGCA AAAAGAAGGT 
GCCGCTGCTA TTCAGAGTGG GATTCTGCCA CTCATCTCTG TGGCTGTAAG TCTGGTGTTG 
GCCAGTCGGT AA