MED25 cDNA ORF clone, Pan paniscus(pygmy chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED25
Entrez Gene ID	100990605
Full Name	mediator complex subunit 25
Gene Type	protein-coding
Organism	Pan paniscus(pygmy chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008966986.1	XP_008965234.1	LOW QUALITY PROTEIN: mediator of RNA polymerase II transcription subunit 25

Pathways from BioSystems
Gene Ontology

The following MED25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa72542	XM_008966986.1 Latest version!	Pan paniscus mediator complex subunit 25 (MED25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED25 ( XM_008966986.1 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_008965234.1 Pan paniscus mediator complex subunit 25 (MED25), mRNA.

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CloneID	OPa72542
Clone ID Related Accession (Same CDS sequence)	XM_008966986.1
Accession Version	XM_008966986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-30
Organism	Pan paniscus(pygmy chimpanzee)
Product	LOW QUALITY PROTEIN: mediator of RNA polymerase II transcription subunit 25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_027887.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan paniscus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCAGCAT TCCCAGCAGC CCTAAACCGC CCGACGCGGG CGCGCGCGCG GCGCAGGCGC 
ATTTCCGCTC ATTCCGCGGC GTCGGCTGCG GCGGCAGTGG CGGTGGCGGG TACCGCACGG 
GGTATGGTCC CCGGGTCCGA GGGCCCGGCC CGCGCCGGGA GCGTGGTGGC CGACGTGGTG 
TTTGTGATTG AGGGTACGGC CAACCTGGGA CCCTACTTCG AGGGGCTCCG CAAGCACTAC 
CTGCTCCCGG CCATCGAGTA TTTTAATGGT GGTCCTCCTG CTGAGACGGA CTTCGGGGGA 
GACTATGGGG GGACCCAGTA CAGCCTCGTG GTGTTCAACA CAGTGGACTG CGCTCCCGAG 
TCCTACGTAC AATGTCACGC TCCCACCAGC AGCGCCTATG AGTTTGTCAC CTGGCTCGAT 
GGCATTAAGT TCATGGGCGG GGGTGGTGAG AGCTGCAGCC TCATCGCGGA AGGACTCAGC 
ACAGCCTTGC AGCTGTTTGA TGACTTCAAG AAGATGCGCG AGCAGATTGG CCAGACGCAC 
CGGGTCTGCC TCCTCATCTG CAACTCACCC CCATACTTGT TGCCTGCTGT TGAGAGCACC 
ACGTACTCTG GATGCACAAC TGAGAATCTT GTGCAGCAGA TTGGGGAGCG GGGGATCCAC 
TTCTCCATCG TGTCTCCCCG GAAGCTGCCT GCGCTTCGGC TTCTGTTTGA GAAGGCAGCC 
CCTCCGGCCT TGCTGGAGCC GCTGCAGCCT CCGACAGATG TGAGCCAGGA CCCGAGGCAC 
ATGGTGCTGG TTCGGGGACT CGTGCTGCCT GTTGGGGGTG GCTCAGCCCC AGGCCCCCTC 
CAGTCAAAGC AGCCAGTCCC CCTGCCTCCC GCCACACCCT CAGGTGCCAC TCTCTCAGCA 
GCTCCCCAGC AGCCTCTGCC CCCCGTCCCC CCGCAGTACC AGGTTCCCGG GAACCTGAGT 
GCAGCTCAGG TGGCTGCGCA GAATGCAGTG GAGGCTGCCA AGAACCAGAA GGCTGGGCTG 
GGCCCTCGCT TCTCGCCCAT CACCCCTCTC CAACAAGCTG CTCCCGGAGT GGGTCCCCCC 
TTCAGCCAGG CCCCAGCTCC CCAGCTACCC CCAGGACCCC CTGGCGCCCC CAAGCCACCA 
CCTGCTTCCC AGCCCAGTCT GGTCTCCACC GTGGCCCCTG GCTCCGGCCT GGCTCCCACG 
GCACAGCCCG GGGCACCGTC CATGGCAGGC ACTGTGGCCC CAGGAGGGGT GAGCGGCCCT 
TCCCCAGCCC AGCTGGGAGC CCCAGCCCTC GGTGGGCAGC AGTCAGTCTC CAATAAGCTT 
CTGGCCTGGA GCGGGGTCTT GGAGTGGCAA GAGAAACCCA AACCTGCCTC AGTGGATGCC 
AACACCAAGC TGACGCGGTC ACTGCCCTGC CAGGTCTACG TGAATCATGG CGAGAACCTG 
AAGACGGAGC AGTGGCCCCA GAAGCTGATC ATGCAGCTCA TCCCCCAGCA GCTGCTGACC 
ACCCTGGGCC CTTTGTTCCG GAACTCAAGG ATGGTCCAGT TCCATTTCAC CAACAAGGAC 
CTGGAGTCTC TCAAAGGCCT CTACCGCATC ATGGGCAACG GCTTCGCGGG CTGCGTGCAC 
TTCCCCCACA CGGCGCCCTG TGAGGTGCGT GTGCTCATGC TCCTGTACTC GTCCAAGAAG 
AAGATCTTCA TGGGCCTCAT CCCCTACGAC CAGAGCGGCT TCGTCAACGG CATCCGGCAG 
GTCATCACCA ACCACAAGCA GGTCCAGCAG CAGAAGCTGG AGCAGCAGCA GCGAGGAATG 
GGGGGACAGC AGGCACCCCC AGGGCTGGGG CCCATTCTGG AGGACCAAGC AAGGCCCTCA 
CAGAATCTGC TCCAGCTCCG CCCACCACAG CCCCAGCCTC AGGGTACCGT AGGGGCCTCT 
GGGGCCACGG GGCAGCCCCA GCCCCAAGGT ACTGCCCAGC CCCCCCCAGG TGCCCCCCAA 
GGCCCTCCTG GAGCAGCTTC TGGCCCACCC CCTCCTGGAC CCATCCTTCG GCCCCAGAAC 
CCTGGGGCCA ACCCCCAGCT GCGAAGCCTC CTCCTCAACC CACCACCGCC GCAGACTGGG 
GTGCCCCCAC CCCAGGCCTC CCTCCACCAC CTCCAGCCAC CAGGGGCTCC TGCGCTGCTG 
CCTCCGCCGC ACCAGGGCCT GGGGCAGCCC CAGTTGGGGC CCCCACTCCT GCATCCACCA 
CCTGCCCAGT CCTGGCCCGC ACAACTTCCC CCGCGGGCTC CACTGCCAGG TCAGATGCTG 
CTGAGCGGGG GTCCCCGGGG CCCGGTCCCC CAGCCGGGCC TGCAGCCCAG CGTCATGGAG 
GACGACATCC TCATGGATCT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008965234.1
CDS	155..2521
Translation	MAAFPAALNRPTRARARRRRISAHSAASAAAAVAVAGTARGMVPGSEGPARAGSVVADVVFVIEGTANLGPYFEGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTENLVQQIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQPPTDVSQDPRHMVLVRGLVLPVGGGSAPGPLQSKQPVPLPPATPSGATLSAAPQQPLPPVPPQYQVPGNLSAAQVAAQNAVEAAKNQKAGLGPRFSPITPLQQAAPGVGPPFSQAPAPQLPPGPPGAPKPPPASQPSLVSTVAPGSGLAPTAQPGAPSMAGTVAPGGVSGPSPAQLGAPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQQRGMGGQQAPPGLGPILEDQARPSQNLLQLRPPQPQPQGTVGASGATGQPQPQGTAQPPPGAPQGPPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPGAPALLPPPHQGLGQPQLGPPLLHPPPAQSWPAQLPPRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	XM_008966986.1
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus mediator complex subunit 25 (MED25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008966986.1

ORF Insert Sequence:

ATGGCAGCAT TCCCAGCAGC CCTAAACCGC CCGACGCGGG CGCGCGCGCG GCGCAGGCGC 
ATTTCCGCTC ATTCCGCGGC GTCGGCTGCG GCGGCAGTGG CGGTGGCGGG TACCGCACGG 
GGTATGGTCC CCGGGTCCGA GGGCCCGGCC CGCGCCGGGA GCGTGGTGGC CGACGTGGTG 
TTTGTGATTG AGGGTACGGC CAACCTGGGA CCCTACTTCG AGGGGCTCCG CAAGCACTAC 
CTGCTCCCGG CCATCGAGTA TTTTAATGGT GGTCCTCCTG CTGAGACGGA CTTCGGGGGA 
GACTATGGGG GGACCCAGTA CAGCCTCGTG GTGTTCAACA CAGTGGACTG CGCTCCCGAG 
TCCTACGTAC AATGTCACGC TCCCACCAGC AGCGCCTATG AGTTTGTCAC CTGGCTCGAT 
GGCATTAAGT TCATGGGCGG GGGTGGTGAG AGCTGCAGCC TCATCGCGGA AGGACTCAGC 
ACAGCCTTGC AGCTGTTTGA TGACTTCAAG AAGATGCGCG AGCAGATTGG CCAGACGCAC 
CGGGTCTGCC TCCTCATCTG CAACTCACCC CCATACTTGT TGCCTGCTGT TGAGAGCACC 
ACGTACTCTG GATGCACAAC TGAGAATCTT GTGCAGCAGA TTGGGGAGCG GGGGATCCAC 
TTCTCCATCG TGTCTCCCCG GAAGCTGCCT GCGCTTCGGC TTCTGTTTGA GAAGGCAGCC 
CCTCCGGCCT TGCTGGAGCC GCTGCAGCCT CCGACAGATG TGAGCCAGGA CCCGAGGCAC 
ATGGTGCTGG TTCGGGGACT CGTGCTGCCT GTTGGGGGTG GCTCAGCCCC AGGCCCCCTC 
CAGTCAAAGC AGCCAGTCCC CCTGCCTCCC GCCACACCCT CAGGTGCCAC TCTCTCAGCA 
GCTCCCCAGC AGCCTCTGCC CCCCGTCCCC CCGCAGTACC AGGTTCCCGG GAACCTGAGT 
GCAGCTCAGG TGGCTGCGCA GAATGCAGTG GAGGCTGCCA AGAACCAGAA GGCTGGGCTG 
GGCCCTCGCT TCTCGCCCAT CACCCCTCTC CAACAAGCTG CTCCCGGAGT GGGTCCCCCC 
TTCAGCCAGG CCCCAGCTCC CCAGCTACCC CCAGGACCCC CTGGCGCCCC CAAGCCACCA 
CCTGCTTCCC AGCCCAGTCT GGTCTCCACC GTGGCCCCTG GCTCCGGCCT GGCTCCCACG 
GCACAGCCCG GGGCACCGTC CATGGCAGGC ACTGTGGCCC CAGGAGGGGT GAGCGGCCCT 
TCCCCAGCCC AGCTGGGAGC CCCAGCCCTC GGTGGGCAGC AGTCAGTCTC CAATAAGCTT 
CTGGCCTGGA GCGGGGTCTT GGAGTGGCAA GAGAAACCCA AACCTGCCTC AGTGGATGCC 
AACACCAAGC TGACGCGGTC ACTGCCCTGC CAGGTCTACG TGAATCATGG CGAGAACCTG 
AAGACGGAGC AGTGGCCCCA GAAGCTGATC ATGCAGCTCA TCCCCCAGCA GCTGCTGACC 
ACCCTGGGCC CTTTGTTCCG GAACTCAAGG ATGGTCCAGT TCCATTTCAC CAACAAGGAC 
CTGGAGTCTC TCAAAGGCCT CTACCGCATC ATGGGCAACG GCTTCGCGGG CTGCGTGCAC 
TTCCCCCACA CGGCGCCCTG TGAGGTGCGT GTGCTCATGC TCCTGTACTC GTCCAAGAAG 
AAGATCTTCA TGGGCCTCAT CCCCTACGAC CAGAGCGGCT TCGTCAACGG CATCCGGCAG 
GTCATCACCA ACCACAAGCA GGTCCAGCAG CAGAAGCTGG AGCAGCAGCA GCGAGGAATG 
GGGGGACAGC AGGCACCCCC AGGGCTGGGG CCCATTCTGG AGGACCAAGC AAGGCCCTCA 
CAGAATCTGC TCCAGCTCCG CCCACCACAG CCCCAGCCTC AGGGTACCGT AGGGGCCTCT 
GGGGCCACGG GGCAGCCCCA GCCCCAAGGT ACTGCCCAGC CCCCCCCAGG TGCCCCCCAA 
GGCCCTCCTG GAGCAGCTTC TGGCCCACCC CCTCCTGGAC CCATCCTTCG GCCCCAGAAC 
CCTGGGGCCA ACCCCCAGCT GCGAAGCCTC CTCCTCAACC CACCACCGCC GCAGACTGGG 
GTGCCCCCAC CCCAGGCCTC CCTCCACCAC CTCCAGCCAC CAGGGGCTCC TGCGCTGCTG 
CCTCCGCCGC ACCAGGGCCT GGGGCAGCCC CAGTTGGGGC CCCCACTCCT GCATCCACCA 
CCTGCCCAGT CCTGGCCCGC ACAACTTCCC CCGCGGGCTC CACTGCCAGG TCAGATGCTG 
CTGAGCGGGG GTCCCCGGGG CCCGGTCCCC CAGCCGGGCC TGCAGCCCAG CGTCATGGAG 
GACGACATCC TCATGGATCT CATCTGA