ITSN2 gene cDNA ORF clone, Ovis aries(sheep)

mRNA	Protein	Name
XM_012165614.2	XP_012021004.1	intersectin-2 isoform X2
XM_012165654.2	XP_012021044.1	intersectin-2 isoform X3
XM_012139899.2	XP_011995289.1	intersectin-2 isoform X1
XM_027966548.1	XP_027822349.1	intersectin-2 isoform X1
XM_012165618.2	XP_012021008.1	intersectin-2 isoform X2
XM_012165644.2	XP_012021034.1	intersectin-2 isoform X1
XM_012139898.2	XP_011995288.1	intersectin-2 isoform X1
XM_012165623.3	XP_012021013.2	intersectin-2 isoform X1
XM_012165623.2	XP_012021013.1	intersectin-2 isoform X2
XM_012139896.2	XP_011995286.1	intersectin-2 isoform X1
XM_012139900.2	XP_011995290.1	intersectin-2 isoform X1
XM_012139904.2	XP_011995294.1	intersectin-2 isoform X2
XM_027966549.1	XP_027822350.1	intersectin-2 isoform X2
XM_027966551.1	XP_027822352.1	intersectin-2 isoform X3
XM_012139902.2	XP_011995292.1	intersectin-2 isoform X4
XM_012165629.2	XP_012021019.1	intersectin-2 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSm76896	XM_027966551.1 Latest version!	Ovis aries intersectin 2 (ITSN2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOv19717	XM_012139896.2 Latest version!	Ovis aries musimon intersectin 2 (ITSN2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv19717	XM_012139899.2 Latest version!	Ovis aries musimon intersectin 2 (ITSN2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv19717	XM_012139898.2 Latest version!	Ovis aries musimon intersectin 2 (ITSN2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv19717	XM_012139900.2 Latest version!	Ovis aries musimon intersectin 2 (ITSN2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv19718	XM_012139902.2 Latest version!	Ovis aries musimon intersectin 2 (ITSN2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv19719	XM_012139904.2 Latest version!	Ovis aries musimon intersectin 2 (ITSN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSm76894	XM_012165623.3 Latest version!	Ovis aries intersectin 2 (ITSN2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm76894	XM_027966548.1 Latest version!	Ovis aries intersectin 2 (ITSN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm76895	XM_027966549.1 Latest version!	Ovis aries intersectin 2 (ITSN2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm29488	XM_012165623.2 Latest version!	Ovis aries intersectin 2 (ITSN2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSm29488	XM_012165614.2 Latest version!	Ovis aries intersectin 2 (ITSN2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSm29488	XM_012165629.2 Latest version!	Ovis aries intersectin 2 (ITSN2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSm29488	XM_012165618.2 Latest version!	Ovis aries intersectin 2 (ITSN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSm29489	XM_012165644.2 Latest version!	Ovis aries intersectin 2 (ITSN2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OSm29490	XM_012165654.2 Latest version!	Ovis aries intersectin 2 (ITSN2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ITSN2 ( XM_027966551.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027822352.1 Ovis aries intersectin 2 (ITSN2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSm76896
Clone ID Related Accession (Same CDS sequence)	XM_027966551.1
Accession Version	XM_027966551.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	intersectin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCCGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAGCAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCAG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTGGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027822352.1
CDS	115..3642
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQSLPPVAPIATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTTSLIQPLSIPYSSSTLPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKCGNVDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGLGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQ

Target ORF information:

RefSeq Version	XM_027966551.1
Organism	Ovis aries(sheep)
Definition	Ovis aries intersectin 2 (ITSN2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966551.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCCGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAGCAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCAG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTGGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_012139899.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011995289.1 Ovis aries musimon intersectin 2 (ITSN2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OOv19717
Clone ID Related Accession (Same CDS sequence)	XM_012139899.2 , XM_012139898.2 , XM_012139896.2 , XM_012139900.2
Accession Version	XM_012139899.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942460.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012139899.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG 
ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG 
AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA 
AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA 
AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG 
GTGAATTACA GAGCATTGTA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT 
TCTGGGGATA TAATACAGGT TGACGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT 
GGTTTTCAAG GAAATTTTGG CTGGTTTCCA TGCAATTATG TAGAAAAAGT GACATCAAGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT 
TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGACTG ATTATCAAAA TGTATCTTTT 
TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC 
CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TGGAAAACCT GAAAGCACAG 
GCCCTTTGTT CTTGGACAGC AAAGAAGGAA AACCACTTGA ACTTCTCAAA ACATGATATC 
ATTACTGTTT TGGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA 
TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA 
GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTACCTCTG CAGCCTGTAC AGTGGGAGAA 
GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA 
GGTGAAGAAC TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC 
AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT 
GCTAGCAAAT CTGGAACATC AAACAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT 
GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG 
AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA 
GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTACACCT 
GCCTTTCATC CTGTATGTCA GGTGATTGCC ATGTATGACT ACACAGCAAA CAACGAGGAT 
GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTTTAA ACAAGGATGA TCCTGACTGG 
TGGCAAGGAG AAATCAGTGG GGTGACTGGT CTCTTCCCTT CGAACTATGT TAAGATGACG 
ACGGATGCAG ATCCAAGTCA GCAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG 
CCGGTGGAAA GGAAGAGGCA GGGCTACATC CATGAGCTGA TTGAGACGGA GGAGCGCTAT 
GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT 
CTCACTGAAG GAGAGATGGC CTTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC 
ACGAAACTGC TGAAGGCACT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG 
CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC 
TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC 
TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC 
AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT 
CTGGAGAACA CCCCAGAGAA TCACGTAGAC CACTCCTCTC TGAAGCAGGC CCTCGAGCGG 
GCAGAGGAGC TCTGTTCTCA AGTGAATGAA GGCGTTCGGG AGAAGGAAAA TTCAGACCGA 
CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTCG CGGAGCAACT GGTTTTCAAC 
TCCCTTACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GCGGAAAGCT GTACAAGACC 
AAGAGCAACA AGGAGCTGCA CGGGTTCCTC TTCAATGACT TCCTGCTTCT CACCTACATG 
GTCAAGCAGT TTGCGGTCTC CTCAGCCTCC GAGAAGCTTT TCAGCTCGAA GTCCAATGCT 
CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAACGAAG CCTTGGTGAA ACTGCCCACA 
GACCCTTCCA GCGATGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC 
CGAACAGACA ACATTAACGA GAGGACAGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG 
CAGTACATCG ACACGGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCCCG CTCTCAGAAG 
TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT 
AAACCAAATG GAAAGAGCAA TCCATACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC 
ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT 
AAGGACCTCT ATCAGGATGT GCTCTGTCTT ACCATGTTTG ACAGAGATCA GTTTTCACCA 
GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAGGAAAGC 
AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACTGGGGA GGTCTGGGTC 
CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011995289.1
CDS	82..5043
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQSLPPVAPIATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTTSLIQPLSIPYSSSTLPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGFGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_012139899.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon intersectin 2 (ITSN2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012139899.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG 
ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG 
AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA 
AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA 
AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG 
GTGAATTACA GAGCATTGTA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT 
TCTGGGGATA TAATACAGGT TGACGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT 
GGTTTTCAAG GAAATTTTGG CTGGTTTCCA TGCAATTATG TAGAAAAAGT GACATCAAGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT 
TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGACTG ATTATCAAAA TGTATCTTTT 
TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC 
CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TGGAAAACCT GAAAGCACAG 
GCCCTTTGTT CTTGGACAGC AAAGAAGGAA AACCACTTGA ACTTCTCAAA ACATGATATC 
ATTACTGTTT TGGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA 
TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA 
GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTACCTCTG CAGCCTGTAC AGTGGGAGAA 
GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA 
GGTGAAGAAC TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC 
AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT 
GCTAGCAAAT CTGGAACATC AAACAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT 
GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG 
AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA 
GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTACACCT 
GCCTTTCATC CTGTATGTCA GGTGATTGCC ATGTATGACT ACACAGCAAA CAACGAGGAT 
GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTTTAA ACAAGGATGA TCCTGACTGG 
TGGCAAGGAG AAATCAGTGG GGTGACTGGT CTCTTCCCTT CGAACTATGT TAAGATGACG 
ACGGATGCAG ATCCAAGTCA GCAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG 
CCGGTGGAAA GGAAGAGGCA GGGCTACATC CATGAGCTGA TTGAGACGGA GGAGCGCTAT 
GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT 
CTCACTGAAG GAGAGATGGC CTTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC 
ACGAAACTGC TGAAGGCACT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG 
CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC 
TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC 
TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC 
AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT 
CTGGAGAACA CCCCAGAGAA TCACGTAGAC CACTCCTCTC TGAAGCAGGC CCTCGAGCGG 
GCAGAGGAGC TCTGTTCTCA AGTGAATGAA GGCGTTCGGG AGAAGGAAAA TTCAGACCGA 
CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTCG CGGAGCAACT GGTTTTCAAC 
TCCCTTACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GCGGAAAGCT GTACAAGACC 
AAGAGCAACA AGGAGCTGCA CGGGTTCCTC TTCAATGACT TCCTGCTTCT CACCTACATG 
GTCAAGCAGT TTGCGGTCTC CTCAGCCTCC GAGAAGCTTT TCAGCTCGAA GTCCAATGCT 
CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAACGAAG CCTTGGTGAA ACTGCCCACA 
GACCCTTCCA GCGATGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC 
CGAACAGACA ACATTAACGA GAGGACAGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG 
CAGTACATCG ACACGGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCCCG CTCTCAGAAG 
TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT 
AAACCAAATG GAAAGAGCAA TCCATACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC 
ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT 
AAGGACCTCT ATCAGGATGT GCTCTGTCTT ACCATGTTTG ACAGAGATCA GTTTTCACCA 
GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAGGAAAGC 
AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACTGGGGA GGTCTGGGTC 
CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_012139898.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011995288.1 Ovis aries musimon intersectin 2 (ITSN2), transcript variant X9, mRNA.

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CloneID	OOv19717
Clone ID Related Accession (Same CDS sequence)	XM_012139899.2 , XM_012139898.2 , XM_012139896.2 , XM_012139900.2
Accession Version	XM_012139898.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942460.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012139898.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG 
ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG 
AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA 
AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA 
AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG 
GTGAATTACA GAGCATTGTA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT 
TCTGGGGATA TAATACAGGT TGACGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT 
GGTTTTCAAG GAAATTTTGG CTGGTTTCCA TGCAATTATG TAGAAAAAGT GACATCAAGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT 
TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGACTG ATTATCAAAA TGTATCTTTT 
TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC 
CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TGGAAAACCT GAAAGCACAG 
GCCCTTTGTT CTTGGACAGC AAAGAAGGAA AACCACTTGA ACTTCTCAAA ACATGATATC 
ATTACTGTTT TGGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA 
TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA 
GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTACCTCTG CAGCCTGTAC AGTGGGAGAA 
GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA 
GGTGAAGAAC TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC 
AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT 
GCTAGCAAAT CTGGAACATC AAACAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT 
GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG 
AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA 
GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTACACCT 
GCCTTTCATC CTGTATGTCA GGTGATTGCC ATGTATGACT ACACAGCAAA CAACGAGGAT 
GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTTTAA ACAAGGATGA TCCTGACTGG 
TGGCAAGGAG AAATCAGTGG GGTGACTGGT CTCTTCCCTT CGAACTATGT TAAGATGACG 
ACGGATGCAG ATCCAAGTCA GCAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG 
CCGGTGGAAA GGAAGAGGCA GGGCTACATC CATGAGCTGA TTGAGACGGA GGAGCGCTAT 
GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT 
CTCACTGAAG GAGAGATGGC CTTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC 
ACGAAACTGC TGAAGGCACT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG 
CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC 
TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC 
TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC 
AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT 
CTGGAGAACA CCCCAGAGAA TCACGTAGAC CACTCCTCTC TGAAGCAGGC CCTCGAGCGG 
GCAGAGGAGC TCTGTTCTCA AGTGAATGAA GGCGTTCGGG AGAAGGAAAA TTCAGACCGA 
CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTCG CGGAGCAACT GGTTTTCAAC 
TCCCTTACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GCGGAAAGCT GTACAAGACC 
AAGAGCAACA AGGAGCTGCA CGGGTTCCTC TTCAATGACT TCCTGCTTCT CACCTACATG 
GTCAAGCAGT TTGCGGTCTC CTCAGCCTCC GAGAAGCTTT TCAGCTCGAA GTCCAATGCT 
CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAACGAAG CCTTGGTGAA ACTGCCCACA 
GACCCTTCCA GCGATGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC 
CGAACAGACA ACATTAACGA GAGGACAGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG 
CAGTACATCG ACACGGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCCCG CTCTCAGAAG 
TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT 
AAACCAAATG GAAAGAGCAA TCCATACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC 
ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT 
AAGGACCTCT ATCAGGATGT GCTCTGTCTT ACCATGTTTG ACAGAGATCA GTTTTCACCA 
GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAGGAAAGC 
AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACTGGGGA GGTCTGGGTC 
CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011995288.1
CDS	1939..6900
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQSLPPVAPIATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTTSLIQPLSIPYSSSTLPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGFGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_012139898.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon intersectin 2 (ITSN2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012139898.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG 
ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG 
AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA 
AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA 
AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG 
GTGAATTACA GAGCATTGTA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT 
TCTGGGGATA TAATACAGGT TGACGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT 
GGTTTTCAAG GAAATTTTGG CTGGTTTCCA TGCAATTATG TAGAAAAAGT GACATCAAGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT 
TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGACTG ATTATCAAAA TGTATCTTTT 
TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC 
CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TGGAAAACCT GAAAGCACAG 
GCCCTTTGTT CTTGGACAGC AAAGAAGGAA AACCACTTGA ACTTCTCAAA ACATGATATC 
ATTACTGTTT TGGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA 
TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA 
GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTACCTCTG CAGCCTGTAC AGTGGGAGAA 
GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA 
GGTGAAGAAC TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC 
AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT 
GCTAGCAAAT CTGGAACATC AAACAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT 
GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG 
AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA 
GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTACACCT 
GCCTTTCATC CTGTATGTCA GGTGATTGCC ATGTATGACT ACACAGCAAA CAACGAGGAT 
GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTTTAA ACAAGGATGA TCCTGACTGG 
TGGCAAGGAG AAATCAGTGG GGTGACTGGT CTCTTCCCTT CGAACTATGT TAAGATGACG 
ACGGATGCAG ATCCAAGTCA GCAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG 
CCGGTGGAAA GGAAGAGGCA GGGCTACATC CATGAGCTGA TTGAGACGGA GGAGCGCTAT 
GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT 
CTCACTGAAG GAGAGATGGC CTTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC 
ACGAAACTGC TGAAGGCACT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG 
CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC 
TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC 
TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC 
AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT 
CTGGAGAACA CCCCAGAGAA TCACGTAGAC CACTCCTCTC TGAAGCAGGC CCTCGAGCGG 
GCAGAGGAGC TCTGTTCTCA AGTGAATGAA GGCGTTCGGG AGAAGGAAAA TTCAGACCGA 
CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTCG CGGAGCAACT GGTTTTCAAC 
TCCCTTACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GCGGAAAGCT GTACAAGACC 
AAGAGCAACA AGGAGCTGCA CGGGTTCCTC TTCAATGACT TCCTGCTTCT CACCTACATG 
GTCAAGCAGT TTGCGGTCTC CTCAGCCTCC GAGAAGCTTT TCAGCTCGAA GTCCAATGCT 
CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAACGAAG CCTTGGTGAA ACTGCCCACA 
GACCCTTCCA GCGATGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC 
CGAACAGACA ACATTAACGA GAGGACAGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG 
CAGTACATCG ACACGGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCCCG CTCTCAGAAG 
TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT 
AAACCAAATG GAAAGAGCAA TCCATACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC 
ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT 
AAGGACCTCT ATCAGGATGT GCTCTGTCTT ACCATGTTTG ACAGAGATCA GTTTTCACCA 
GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAGGAAAGC 
AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACTGGGGA GGTCTGGGTC 
CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_012139896.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011995286.1 Ovis aries musimon intersectin 2 (ITSN2), transcript variant X8, mRNA.

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CloneID	OOv19717
Clone ID Related Accession (Same CDS sequence)	XM_012139899.2 , XM_012139898.2 , XM_012139896.2 , XM_012139900.2
Accession Version	XM_012139896.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942460.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012139896.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG 
ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG 
AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA 
AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA 
AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG 
GTGAATTACA GAGCATTGTA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT 
TCTGGGGATA TAATACAGGT TGACGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT 
GGTTTTCAAG GAAATTTTGG CTGGTTTCCA TGCAATTATG TAGAAAAAGT GACATCAAGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT 
TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGACTG ATTATCAAAA TGTATCTTTT 
TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC 
CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TGGAAAACCT GAAAGCACAG 
GCCCTTTGTT CTTGGACAGC AAAGAAGGAA AACCACTTGA ACTTCTCAAA ACATGATATC 
ATTACTGTTT TGGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA 
TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA 
GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTACCTCTG CAGCCTGTAC AGTGGGAGAA 
GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA 
GGTGAAGAAC TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC 
AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT 
GCTAGCAAAT CTGGAACATC AAACAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT 
GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG 
AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA 
GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTACACCT 
GCCTTTCATC CTGTATGTCA GGTGATTGCC ATGTATGACT ACACAGCAAA CAACGAGGAT 
GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTTTAA ACAAGGATGA TCCTGACTGG 
TGGCAAGGAG AAATCAGTGG GGTGACTGGT CTCTTCCCTT CGAACTATGT TAAGATGACG 
ACGGATGCAG ATCCAAGTCA GCAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG 
CCGGTGGAAA GGAAGAGGCA GGGCTACATC CATGAGCTGA TTGAGACGGA GGAGCGCTAT 
GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT 
CTCACTGAAG GAGAGATGGC CTTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC 
ACGAAACTGC TGAAGGCACT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG 
CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC 
TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC 
TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC 
AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT 
CTGGAGAACA CCCCAGAGAA TCACGTAGAC CACTCCTCTC TGAAGCAGGC CCTCGAGCGG 
GCAGAGGAGC TCTGTTCTCA AGTGAATGAA GGCGTTCGGG AGAAGGAAAA TTCAGACCGA 
CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTCG CGGAGCAACT GGTTTTCAAC 
TCCCTTACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GCGGAAAGCT GTACAAGACC 
AAGAGCAACA AGGAGCTGCA CGGGTTCCTC TTCAATGACT TCCTGCTTCT CACCTACATG 
GTCAAGCAGT TTGCGGTCTC CTCAGCCTCC GAGAAGCTTT TCAGCTCGAA GTCCAATGCT 
CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAACGAAG CCTTGGTGAA ACTGCCCACA 
GACCCTTCCA GCGATGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC 
CGAACAGACA ACATTAACGA GAGGACAGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG 
CAGTACATCG ACACGGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCCCG CTCTCAGAAG 
TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT 
AAACCAAATG GAAAGAGCAA TCCATACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC 
ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT 
AAGGACCTCT ATCAGGATGT GCTCTGTCTT ACCATGTTTG ACAGAGATCA GTTTTCACCA 
GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAGGAAAGC 
AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACTGGGGA GGTCTGGGTC 
CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011995286.1
CDS	201..5162
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQSLPPVAPIATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTTSLIQPLSIPYSSSTLPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGFGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_012139896.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon intersectin 2 (ITSN2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012139896.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG 
ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG 
AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA 
AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA 
AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG 
GTGAATTACA GAGCATTGTA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT 
TCTGGGGATA TAATACAGGT TGACGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT 
GGTTTTCAAG GAAATTTTGG CTGGTTTCCA TGCAATTATG TAGAAAAAGT GACATCAAGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT 
TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGACTG ATTATCAAAA TGTATCTTTT 
TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC 
CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TGGAAAACCT GAAAGCACAG 
GCCCTTTGTT CTTGGACAGC AAAGAAGGAA AACCACTTGA ACTTCTCAAA ACATGATATC 
ATTACTGTTT TGGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA 
TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA 
GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTACCTCTG CAGCCTGTAC AGTGGGAGAA 
GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA 
GGTGAAGAAC TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC 
AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT 
GCTAGCAAAT CTGGAACATC AAACAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT 
GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG 
AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA 
GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTACACCT 
GCCTTTCATC CTGTATGTCA GGTGATTGCC ATGTATGACT ACACAGCAAA CAACGAGGAT 
GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTTTAA ACAAGGATGA TCCTGACTGG 
TGGCAAGGAG AAATCAGTGG GGTGACTGGT CTCTTCCCTT CGAACTATGT TAAGATGACG 
ACGGATGCAG ATCCAAGTCA GCAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG 
CCGGTGGAAA GGAAGAGGCA GGGCTACATC CATGAGCTGA TTGAGACGGA GGAGCGCTAT 
GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT 
CTCACTGAAG GAGAGATGGC CTTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC 
ACGAAACTGC TGAAGGCACT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG 
CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC 
TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC 
TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC 
AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT 
CTGGAGAACA CCCCAGAGAA TCACGTAGAC CACTCCTCTC TGAAGCAGGC CCTCGAGCGG 
GCAGAGGAGC TCTGTTCTCA AGTGAATGAA GGCGTTCGGG AGAAGGAAAA TTCAGACCGA 
CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTCG CGGAGCAACT GGTTTTCAAC 
TCCCTTACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GCGGAAAGCT GTACAAGACC 
AAGAGCAACA AGGAGCTGCA CGGGTTCCTC TTCAATGACT TCCTGCTTCT CACCTACATG 
GTCAAGCAGT TTGCGGTCTC CTCAGCCTCC GAGAAGCTTT TCAGCTCGAA GTCCAATGCT 
CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAACGAAG CCTTGGTGAA ACTGCCCACA 
GACCCTTCCA GCGATGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC 
CGAACAGACA ACATTAACGA GAGGACAGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG 
CAGTACATCG ACACGGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCCCG CTCTCAGAAG 
TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT 
AAACCAAATG GAAAGAGCAA TCCATACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC 
ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT 
AAGGACCTCT ATCAGGATGT GCTCTGTCTT ACCATGTTTG ACAGAGATCA GTTTTCACCA 
GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAGGAAAGC 
AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACTGGGGA GGTCTGGGTC 
CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_012139900.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011995290.1 Ovis aries musimon intersectin 2 (ITSN2), transcript variant X11, mRNA.

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CloneID	OOv19717
Clone ID Related Accession (Same CDS sequence)	XM_012139899.2 , XM_012139898.2 , XM_012139896.2 , XM_012139900.2
Accession Version	XM_012139900.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942460.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012139900.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG 
ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG 
AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA 
AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA 
AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG 
GTGAATTACA GAGCATTGTA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT 
TCTGGGGATA TAATACAGGT TGACGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT 
GGTTTTCAAG GAAATTTTGG CTGGTTTCCA TGCAATTATG TAGAAAAAGT GACATCAAGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT 
TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGACTG ATTATCAAAA TGTATCTTTT 
TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC 
CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TGGAAAACCT GAAAGCACAG 
GCCCTTTGTT CTTGGACAGC AAAGAAGGAA AACCACTTGA ACTTCTCAAA ACATGATATC 
ATTACTGTTT TGGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA 
TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA 
GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTACCTCTG CAGCCTGTAC AGTGGGAGAA 
GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA 
GGTGAAGAAC TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC 
AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT 
GCTAGCAAAT CTGGAACATC AAACAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT 
GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG 
AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA 
GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTACACCT 
GCCTTTCATC CTGTATGTCA GGTGATTGCC ATGTATGACT ACACAGCAAA CAACGAGGAT 
GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTTTAA ACAAGGATGA TCCTGACTGG 
TGGCAAGGAG AAATCAGTGG GGTGACTGGT CTCTTCCCTT CGAACTATGT TAAGATGACG 
ACGGATGCAG ATCCAAGTCA GCAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG 
CCGGTGGAAA GGAAGAGGCA GGGCTACATC CATGAGCTGA TTGAGACGGA GGAGCGCTAT 
GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT 
CTCACTGAAG GAGAGATGGC CTTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC 
ACGAAACTGC TGAAGGCACT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG 
CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC 
TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC 
TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC 
AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT 
CTGGAGAACA CCCCAGAGAA TCACGTAGAC CACTCCTCTC TGAAGCAGGC CCTCGAGCGG 
GCAGAGGAGC TCTGTTCTCA AGTGAATGAA GGCGTTCGGG AGAAGGAAAA TTCAGACCGA 
CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTCG CGGAGCAACT GGTTTTCAAC 
TCCCTTACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GCGGAAAGCT GTACAAGACC 
AAGAGCAACA AGGAGCTGCA CGGGTTCCTC TTCAATGACT TCCTGCTTCT CACCTACATG 
GTCAAGCAGT TTGCGGTCTC CTCAGCCTCC GAGAAGCTTT TCAGCTCGAA GTCCAATGCT 
CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAACGAAG CCTTGGTGAA ACTGCCCACA 
GACCCTTCCA GCGATGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC 
CGAACAGACA ACATTAACGA GAGGACAGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG 
CAGTACATCG ACACGGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCCCG CTCTCAGAAG 
TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT 
AAACCAAATG GAAAGAGCAA TCCATACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC 
ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT 
AAGGACCTCT ATCAGGATGT GCTCTGTCTT ACCATGTTTG ACAGAGATCA GTTTTCACCA 
GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAGGAAAGC 
AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACTGGGGA GGTCTGGGTC 
CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011995290.1
CDS	88..5049
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQSLPPVAPIATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTTSLIQPLSIPYSSSTLPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGFGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_012139900.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon intersectin 2 (ITSN2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012139900.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG 
ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG 
AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA 
AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA 
AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG 
GTGAATTACA GAGCATTGTA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT 
TCTGGGGATA TAATACAGGT TGACGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT 
GGTTTTCAAG GAAATTTTGG CTGGTTTCCA TGCAATTATG TAGAAAAAGT GACATCAAGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT 
TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGACTG ATTATCAAAA TGTATCTTTT 
TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC 
CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TGGAAAACCT GAAAGCACAG 
GCCCTTTGTT CTTGGACAGC AAAGAAGGAA AACCACTTGA ACTTCTCAAA ACATGATATC 
ATTACTGTTT TGGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA 
TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA 
GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTACCTCTG CAGCCTGTAC AGTGGGAGAA 
GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA 
GGTGAAGAAC TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC 
AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT 
GCTAGCAAAT CTGGAACATC AAACAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT 
GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG 
AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA 
GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTACACCT 
GCCTTTCATC CTGTATGTCA GGTGATTGCC ATGTATGACT ACACAGCAAA CAACGAGGAT 
GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTTTAA ACAAGGATGA TCCTGACTGG 
TGGCAAGGAG AAATCAGTGG GGTGACTGGT CTCTTCCCTT CGAACTATGT TAAGATGACG 
ACGGATGCAG ATCCAAGTCA GCAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG 
CCGGTGGAAA GGAAGAGGCA GGGCTACATC CATGAGCTGA TTGAGACGGA GGAGCGCTAT 
GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT 
CTCACTGAAG GAGAGATGGC CTTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC 
ACGAAACTGC TGAAGGCACT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG 
CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC 
TGCAGCTGTC AGCTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC 
TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC 
AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT 
CTGGAGAACA CCCCAGAGAA TCACGTAGAC CACTCCTCTC TGAAGCAGGC CCTCGAGCGG 
GCAGAGGAGC TCTGTTCTCA AGTGAATGAA GGCGTTCGGG AGAAGGAAAA TTCAGACCGA 
CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTCG CGGAGCAACT GGTTTTCAAC 
TCCCTTACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GCGGAAAGCT GTACAAGACC 
AAGAGCAACA AGGAGCTGCA CGGGTTCCTC TTCAATGACT TCCTGCTTCT CACCTACATG 
GTCAAGCAGT TTGCGGTCTC CTCAGCCTCC GAGAAGCTTT TCAGCTCGAA GTCCAATGCT 
CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAACGAAG CCTTGGTGAA ACTGCCCACA 
GACCCTTCCA GCGATGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC 
CGAACAGACA ACATTAACGA GAGGACAGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG 
CAGTACATCG ACACGGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCCCG CTCTCAGAAG 
TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT 
AAACCAAATG GAAAGAGCAA TCCATACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC 
ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT 
AAGGACCTCT ATCAGGATGT GCTCTGTCTT ACCATGTTTG ACAGAGATCA GTTTTCACCA 
GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAGGAAAGC 
AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACTGGGGA GGTCTGGGTC 
CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_012139902.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011995292.1 Ovis aries musimon intersectin 2 (ITSN2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OOv19718
Clone ID Related Accession (Same CDS sequence)	XM_012139902.2
Accession Version	XM_012139902.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	intersectin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942460.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012139902.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGCATTGCCT 
CATACGTCAT CTTACAGTCT GATGATGGGA GGATTTGGTG GTGCTAATAT ACAGAAAGCC 
CAGTCTCTGA TTGATTTAGG ATCTAGTAGC TCAACTTCCT CAACTGCTTC CCTCTCAGGG 
AATTCACCCA AGACTGGGAC CTCAGAGTGG GCAGTTCCTC AGCCTTCAAG ATTAAAATAT 
CGGCAAAAAT TTAACAGTCT TGACAAAAGC ATGAGTGGAT ACCTCTCAGG TTTTCAAGCT 
AGAAATGCTC TTCTTCAGTC AAATCTTTCT CAAACTCAGC TAGCTACTAT ATGGACTCTG 
GCTGACATTG ATGGTGATGG ACAGCTGAAA GCTGAGGAAT TCATCCTTGC AATGCACCTC 
ACTGATATGG CCAAAGCTGG ACAGCCATTG CCACTGGCTT TACCTCCTGA GCTTGTCCCT 
CCATCTTTTA GAGGAGGAAA GCAGATTGAT TCCATTAACG GAACTCTGCC TTCATATCAG 
AAAACACAAG AAGAAGAACC TCAGAAAAAA TTACCAGTTA CGTTCGAGGA CAAGCGGAAA 
GCCAACTATG AGCGGGGCAA CATGGAACTG GAGAAGCGAC GCCAGGCCAT GATGGAGCAG 
CAGCAGAGGG AGGCAGAGCG CAAAGCCCAG AAAGAAAAGG AAGAATGGGA ACGAAAACAG 
AGAGAGCTAC AAGAGCAAGA ATGGAAGAAA CAACTTGAAT TAGAAAAACG CTTGGAGAAG 
CAGAGGGAAT TGGAGCGACA ACGGGAAGAA GAAAGGAGAA AAGAGATAGA AAGACGAGAG 
GCCGCAAAAC AGGAACTTGA AAGACAACGT CGTTTAGAAT GGGAGAGAAT TCGTAGGCAG 
GAGCTTCTCA GTCGAAGGAA TAGAGAACAG GAAGAAATTG TCAGGTTAAA CTCTAAAAAG 
AAGAGCCTCC ATCTCGAGCT GGAAGCACTG AATGGCAAAC ATCAACAGAT CTCAGGCAGA 
CTTCAAGACG TCCGACTCAG AAAGCAAACT CAGAAGACCG AGCTGGAAGT TCTGGATAAG 
CAGTGTGACC TGGAAATCAT GGAAATCAAG CAGCTTCAAC AGGAGCTTCA GGAGTATCAA 
AATAAACTTA TCTATTTGGT GCCAGAGAAG CAGTTATTAA ACGAAAGAAT TAAAAACATG 
CAGCTCAGTA ACACACCTGA TTTAGGGATC AGTTTACTTC ATAAAAAATC ATTAGAAAAG 
GAAGAATTAT GCCAAAGACT TAAAGAACAA TTAGATGCTC TTGAAAAAGA AACTGCATCC 
AAGCTTTCAG AAATGGATTC ATTTAACAAT GAACTGAAGG AACTGAGAGA AAGCTACAAT 
ACACAGCAGT TAGCCATTGA ACAGCTTTAT AAGATCAAAC GTGACAAACT GAGGGAAATT 
GAAAGAAAAA GATCAGAGTT AATACAGAAA AAGAAACTAG AAGATGAGGC TACAAGGAAA 
GCAAAACAAG GAAAAGAAAA CTTATGGAAA GAAAATCTTA GAAAGGAGGA AGAAGAAAAA 
CAAAAACGAC TCCAAGAAGA AAAAGCACAG GAAAAAGTTC AAGAAGTGGA GCGGAAAGCT 
GAGGAGAAAC AAAGTGAGCC AGCTGGTACT TTGGTGAATT ACAGAGCATT GTATCGCTTT 
GAAGCAAGGA ACCATGATGA AATGAGTTTT AATTCTGGGG ATATAATACA GGTTGACGAA 
AAAACTGTAG GAGAACCTGG TTGGCTTTAT GGTGGTTTTC AAGGAAATTT TGGCTGGTTT 
CCATGCAATT ATGTAGAAAA AGTGACATCA AGTGAAAAAT CTGTGTCTCC TAAGAAGGCC 
TTACTTCCTC CTACAGTATC TGCTACCTCA ACTTCTGAAT CACTTTCTTC AAACCAACCA 
GCATCTGTGA CTGATTATCA AAATGTATCT TTTTCAAACC TAAGTGTTAA CACATCATGG 
CAGAAAAAAT CAGCTTTTAC TCGCACTGTG TCCCCTGTGT CTGTATCACC GATTCATGGA 
CAGGGACAGG TTGTGGAAAA CCTGAAAGCA CAGGCCCTTT GTTCTTGGAC AGCAAAGAAG 
GAAAACCACT TGAACTTCTC AAAACATGAT ATCATTACTG TTTTGGAGCA GCAAGAAAAT 
TGGTGGTTTG GGGAGGTTCA CGGAGGAAGA GGATGGTTTC CGAAATCTTA CGTCAAAATC 
ATCCCTGGGA GTGAAGTTAA GCGGGAAGAA CCAGAAGCTT TGTATGCATC TGTAAACAAG 
AAACCTACCT CTGCAGCCTG TACAGTGGGA GAAGAGTATA TTGCACTTTA TTCATATTCA 
AGTGTAGAAC CTGGAGATTT GACTTTCACT GAAGGTGAAG AACTATTGGT GACCCAGAAA 
GATGGAGAAT GGTGGACAGG AAGTATTGGA GACAGAACTG GAATTTTTCC ATCAAATTAT 
GTCAAACCAA AAGATCAGGA GGGTTTTGGG AGTGCTAGCA AATCTGGAAC ATCAAACAAG 
AAACCTGAGA TTGCTCAAGT AACATCAGCA TATGCGGCTT CTGGTTCTGA ACAGCTTAGC 
CTCGCACCAG GACAGTTAAT ACTAATTCTA AAGAAAAACA CAAGTGGGTG GTGGCAAGGG 
GAATTACAGG CCAGAGGGAA AAAGCGACAG AAAGGATGGT TTCCTGCCAG CCATGTTAAA 
CTTTTGGGTC CAAGTAGTGA AAGAACTACA CCTGCCTTTC ATCCTGTATG TCAGGTGATT 
GCCATGTATG ACTACACAGC AAACAACGAG GATGAGCTCA GTTTCTCCAA GGGACAGCTT 
ATTAATGTTT TAAACAAGGA TGATCCTGAC TGGTGGCAAG GAGAAATCAG TGGGGTGACT 
GGTCTCTTCC CTTCGAACTA TGTTAAGATG ACGACGGATG CAGATCCAAG TCAGCAGTGG 
TGTGCTGATC TCCAAACACT GGACACCATG CAGCCGGTGG AAAGGAAGAG GCAGGGCTAC 
ATCCATGAGC TGATTGAGAC GGAGGAGCGC TATGTGGACG ACCTGCAGCT CGTCGTGGAG 
GTTTTTCAGA AACGAATAAC AGAGCCAGGT TTTCTCACTG AAGGAGAGAT GGCCTTGATC 
TTTGTTAACT GGAAGGAGCT CATCATGGCT AACACGAAAC TGCTGAAGGC ACTGCGGGTG 
CGGAAGAAGA CCGGGGGTGA GAAGATGCCC GTGCACATGA TCGGGGACAT CCTGGCCGCT 
GAGCTGTCCC ACATGCAGGC CTACATCCGC TTCTGCAGCT GTCAGCTTAA TGGAGCAGCC 
CTGTTACAGC AGAAGACAGA CGAACACGCA GACTTCAAAG AATTTTTAAA GAAGTTGGCA 
TCTGACCCTC GGTGTAAAGG GATGCCCCTT TCCAGCTTCC TGCTGAAGCC CATGCAGAGG 
ATCACCCGCT ACCCACTCCT CATCAGAAGC ATTCTGGAGA ACACCCCAGA GAATCACGTA 
GACCACTCCT CTCTGAAGCA GGCCCTCGAG CGGGCAGAGG AGCTCTGTTC TCAAGTGAAT 
GAAGGCGTTC GGGAGAAGGA AAATTCAGAC CGACTGGAGT GGATCCAGAC GCACGTGCAG 
TGCGATGGGC TCGCGGAGCA ACTGGTTTTC AACTCCCTTA CCAACTGCCT GGGGCCCCGG 
AAGCTCCTGC ACAGCGGAAA GCTGTACAAG ACCAAGAGCA ACAAGGAGCT GCACGGGTTC 
CTCTTCAATG ACTTCCTGCT TCTCACCTAC ATGGTCAAGC AGTTTGCGGT CTCCTCAGCC 
TCCGAGAAGC TTTTCAGCTC GAAGTCCAAT GCTCAGTTCA AGATGTATAA GACGCCCATT 
TTCCTGAACG AAGCCTTGGT GAAACTGCCC ACAGACCCTT CCAGCGATGA GCCTGTCTTC 
CACATCTCTC ACATTGACCG GGTCTACACC CTCCGAACAG ACAACATTAA CGAGAGGACA 
GCCTGGGTCC AGAAGATCAA GGCCGCGTCT GAGCAGTACA TCGACACGGA AAAGAAGAAG 
CGGGAGAAGG CCTATCAAGC CCGCTCTCAG AAGTCTTCAG GCATCGGGCG TCTGATGGTG 
CATGTCATTG AAGCTACAGA ACTGAAAGCC TGTAAACCAA ATGGAAAGAG CAATCCATAC 
TGTGAAATCA GCATGGGCTC CCAGAGCTAC ACCACCAGAA CCCTCCAGGA CACACTAAAT 
CCCAAATGGA ATTTTAACTG CCAGTTCTTC ATTAAGGACC TCTATCAGGA TGTGCTCTGT 
CTTACCATGT TTGACAGAGA TCAGTTTTCA CCAGACGATT TCCTGGGTCG CACTGAAGTT 
CCAGTGGCAA AAATTCGAAC AGAACAGGAA AGCAAAGGCC CTACAACCCG CCGACTCCTG 
CTGCACGAGG TCCCCACTGG GGAGGTCTGG GTCCGCTTTG ACCTGCAGCT TTTTGAGCAG 
AAAACTCTCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011995292.1
CDS	201..4955
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFALPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGFGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_012139902.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon intersectin 2 (ITSN2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012139902.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGCATTGCCT 
CATACGTCAT CTTACAGTCT GATGATGGGA GGATTTGGTG GTGCTAATAT ACAGAAAGCC 
CAGTCTCTGA TTGATTTAGG ATCTAGTAGC TCAACTTCCT CAACTGCTTC CCTCTCAGGG 
AATTCACCCA AGACTGGGAC CTCAGAGTGG GCAGTTCCTC AGCCTTCAAG ATTAAAATAT 
CGGCAAAAAT TTAACAGTCT TGACAAAAGC ATGAGTGGAT ACCTCTCAGG TTTTCAAGCT 
AGAAATGCTC TTCTTCAGTC AAATCTTTCT CAAACTCAGC TAGCTACTAT ATGGACTCTG 
GCTGACATTG ATGGTGATGG ACAGCTGAAA GCTGAGGAAT TCATCCTTGC AATGCACCTC 
ACTGATATGG CCAAAGCTGG ACAGCCATTG CCACTGGCTT TACCTCCTGA GCTTGTCCCT 
CCATCTTTTA GAGGAGGAAA GCAGATTGAT TCCATTAACG GAACTCTGCC TTCATATCAG 
AAAACACAAG AAGAAGAACC TCAGAAAAAA TTACCAGTTA CGTTCGAGGA CAAGCGGAAA 
GCCAACTATG AGCGGGGCAA CATGGAACTG GAGAAGCGAC GCCAGGCCAT GATGGAGCAG 
CAGCAGAGGG AGGCAGAGCG CAAAGCCCAG AAAGAAAAGG AAGAATGGGA ACGAAAACAG 
AGAGAGCTAC AAGAGCAAGA ATGGAAGAAA CAACTTGAAT TAGAAAAACG CTTGGAGAAG 
CAGAGGGAAT TGGAGCGACA ACGGGAAGAA GAAAGGAGAA AAGAGATAGA AAGACGAGAG 
GCCGCAAAAC AGGAACTTGA AAGACAACGT CGTTTAGAAT GGGAGAGAAT TCGTAGGCAG 
GAGCTTCTCA GTCGAAGGAA TAGAGAACAG GAAGAAATTG TCAGGTTAAA CTCTAAAAAG 
AAGAGCCTCC ATCTCGAGCT GGAAGCACTG AATGGCAAAC ATCAACAGAT CTCAGGCAGA 
CTTCAAGACG TCCGACTCAG AAAGCAAACT CAGAAGACCG AGCTGGAAGT TCTGGATAAG 
CAGTGTGACC TGGAAATCAT GGAAATCAAG CAGCTTCAAC AGGAGCTTCA GGAGTATCAA 
AATAAACTTA TCTATTTGGT GCCAGAGAAG CAGTTATTAA ACGAAAGAAT TAAAAACATG 
CAGCTCAGTA ACACACCTGA TTTAGGGATC AGTTTACTTC ATAAAAAATC ATTAGAAAAG 
GAAGAATTAT GCCAAAGACT TAAAGAACAA TTAGATGCTC TTGAAAAAGA AACTGCATCC 
AAGCTTTCAG AAATGGATTC ATTTAACAAT GAACTGAAGG AACTGAGAGA AAGCTACAAT 
ACACAGCAGT TAGCCATTGA ACAGCTTTAT AAGATCAAAC GTGACAAACT GAGGGAAATT 
GAAAGAAAAA GATCAGAGTT AATACAGAAA AAGAAACTAG AAGATGAGGC TACAAGGAAA 
GCAAAACAAG GAAAAGAAAA CTTATGGAAA GAAAATCTTA GAAAGGAGGA AGAAGAAAAA 
CAAAAACGAC TCCAAGAAGA AAAAGCACAG GAAAAAGTTC AAGAAGTGGA GCGGAAAGCT 
GAGGAGAAAC AAAGTGAGCC AGCTGGTACT TTGGTGAATT ACAGAGCATT GTATCGCTTT 
GAAGCAAGGA ACCATGATGA AATGAGTTTT AATTCTGGGG ATATAATACA GGTTGACGAA 
AAAACTGTAG GAGAACCTGG TTGGCTTTAT GGTGGTTTTC AAGGAAATTT TGGCTGGTTT 
CCATGCAATT ATGTAGAAAA AGTGACATCA AGTGAAAAAT CTGTGTCTCC TAAGAAGGCC 
TTACTTCCTC CTACAGTATC TGCTACCTCA ACTTCTGAAT CACTTTCTTC AAACCAACCA 
GCATCTGTGA CTGATTATCA AAATGTATCT TTTTCAAACC TAAGTGTTAA CACATCATGG 
CAGAAAAAAT CAGCTTTTAC TCGCACTGTG TCCCCTGTGT CTGTATCACC GATTCATGGA 
CAGGGACAGG TTGTGGAAAA CCTGAAAGCA CAGGCCCTTT GTTCTTGGAC AGCAAAGAAG 
GAAAACCACT TGAACTTCTC AAAACATGAT ATCATTACTG TTTTGGAGCA GCAAGAAAAT 
TGGTGGTTTG GGGAGGTTCA CGGAGGAAGA GGATGGTTTC CGAAATCTTA CGTCAAAATC 
ATCCCTGGGA GTGAAGTTAA GCGGGAAGAA CCAGAAGCTT TGTATGCATC TGTAAACAAG 
AAACCTACCT CTGCAGCCTG TACAGTGGGA GAAGAGTATA TTGCACTTTA TTCATATTCA 
AGTGTAGAAC CTGGAGATTT GACTTTCACT GAAGGTGAAG AACTATTGGT GACCCAGAAA 
GATGGAGAAT GGTGGACAGG AAGTATTGGA GACAGAACTG GAATTTTTCC ATCAAATTAT 
GTCAAACCAA AAGATCAGGA GGGTTTTGGG AGTGCTAGCA AATCTGGAAC ATCAAACAAG 
AAACCTGAGA TTGCTCAAGT AACATCAGCA TATGCGGCTT CTGGTTCTGA ACAGCTTAGC 
CTCGCACCAG GACAGTTAAT ACTAATTCTA AAGAAAAACA CAAGTGGGTG GTGGCAAGGG 
GAATTACAGG CCAGAGGGAA AAAGCGACAG AAAGGATGGT TTCCTGCCAG CCATGTTAAA 
CTTTTGGGTC CAAGTAGTGA AAGAACTACA CCTGCCTTTC ATCCTGTATG TCAGGTGATT 
GCCATGTATG ACTACACAGC AAACAACGAG GATGAGCTCA GTTTCTCCAA GGGACAGCTT 
ATTAATGTTT TAAACAAGGA TGATCCTGAC TGGTGGCAAG GAGAAATCAG TGGGGTGACT 
GGTCTCTTCC CTTCGAACTA TGTTAAGATG ACGACGGATG CAGATCCAAG TCAGCAGTGG 
TGTGCTGATC TCCAAACACT GGACACCATG CAGCCGGTGG AAAGGAAGAG GCAGGGCTAC 
ATCCATGAGC TGATTGAGAC GGAGGAGCGC TATGTGGACG ACCTGCAGCT CGTCGTGGAG 
GTTTTTCAGA AACGAATAAC AGAGCCAGGT TTTCTCACTG AAGGAGAGAT GGCCTTGATC 
TTTGTTAACT GGAAGGAGCT CATCATGGCT AACACGAAAC TGCTGAAGGC ACTGCGGGTG 
CGGAAGAAGA CCGGGGGTGA GAAGATGCCC GTGCACATGA TCGGGGACAT CCTGGCCGCT 
GAGCTGTCCC ACATGCAGGC CTACATCCGC TTCTGCAGCT GTCAGCTTAA TGGAGCAGCC 
CTGTTACAGC AGAAGACAGA CGAACACGCA GACTTCAAAG AATTTTTAAA GAAGTTGGCA 
TCTGACCCTC GGTGTAAAGG GATGCCCCTT TCCAGCTTCC TGCTGAAGCC CATGCAGAGG 
ATCACCCGCT ACCCACTCCT CATCAGAAGC ATTCTGGAGA ACACCCCAGA GAATCACGTA 
GACCACTCCT CTCTGAAGCA GGCCCTCGAG CGGGCAGAGG AGCTCTGTTC TCAAGTGAAT 
GAAGGCGTTC GGGAGAAGGA AAATTCAGAC CGACTGGAGT GGATCCAGAC GCACGTGCAG 
TGCGATGGGC TCGCGGAGCA ACTGGTTTTC AACTCCCTTA CCAACTGCCT GGGGCCCCGG 
AAGCTCCTGC ACAGCGGAAA GCTGTACAAG ACCAAGAGCA ACAAGGAGCT GCACGGGTTC 
CTCTTCAATG ACTTCCTGCT TCTCACCTAC ATGGTCAAGC AGTTTGCGGT CTCCTCAGCC 
TCCGAGAAGC TTTTCAGCTC GAAGTCCAAT GCTCAGTTCA AGATGTATAA GACGCCCATT 
TTCCTGAACG AAGCCTTGGT GAAACTGCCC ACAGACCCTT CCAGCGATGA GCCTGTCTTC 
CACATCTCTC ACATTGACCG GGTCTACACC CTCCGAACAG ACAACATTAA CGAGAGGACA 
GCCTGGGTCC AGAAGATCAA GGCCGCGTCT GAGCAGTACA TCGACACGGA AAAGAAGAAG 
CGGGAGAAGG CCTATCAAGC CCGCTCTCAG AAGTCTTCAG GCATCGGGCG TCTGATGGTG 
CATGTCATTG AAGCTACAGA ACTGAAAGCC TGTAAACCAA ATGGAAAGAG CAATCCATAC 
TGTGAAATCA GCATGGGCTC CCAGAGCTAC ACCACCAGAA CCCTCCAGGA CACACTAAAT 
CCCAAATGGA ATTTTAACTG CCAGTTCTTC ATTAAGGACC TCTATCAGGA TGTGCTCTGT 
CTTACCATGT TTGACAGAGA TCAGTTTTCA CCAGACGATT TCCTGGGTCG CACTGAAGTT 
CCAGTGGCAA AAATTCGAAC AGAACAGGAA AGCAAAGGCC CTACAACCCG CCGACTCCTG 
CTGCACGAGG TCCCCACTGG GGAGGTCTGG GTCCGCTTTG ACCTGCAGCT TTTTGAGCAG 
AAAACTCTCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_012139904.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011995294.1 Ovis aries musimon intersectin 2 (ITSN2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOv19719
Clone ID Related Accession (Same CDS sequence)	XM_012139904.2
Accession Version	XM_012139904.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	intersectin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942460.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012139904.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCAG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTTGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGGTG TGCTGATCTC 
CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCATGAGCTG 
ATTGAGACGG AGGAGCGCTA TGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA 
CGAATAACAG AGCCAGGTTT TCTCACTGAA GGAGAGATGG CCTTGATCTT TGTTAACTGG 
AAGGAGCTCA TCATGGCTAA CACGAAACTG CTGAAGGCAC TGCGGGTGCG GAAGAAGACC 
GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC 
ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAGCTTAATG GAGCAGCCCT GTTACAGCAG 
AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG 
TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC 
CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCACGTAGA CCACTCCTCT 
CTGAAGCAGG CCCTCGAGCG GGCAGAGGAG CTCTGTTCTC AAGTGAATGA AGGCGTTCGG 
GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTC 
GCGGAGCAAC TGGTTTTCAA CTCCCTTACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC 
AGCGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTGC ACGGGTTCCT CTTCAATGAC 
TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTTGCGGTCT CCTCAGCCTC CGAGAAGCTT 
TTCAGCTCGA AGTCCAATGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAACGAA 
GCCTTGGTGA AACTGCCCAC AGACCCTTCC AGCGATGAGC CTGTCTTCCA CATCTCTCAC 
ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACAGC CTGGGTCCAG 
AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACGGAAA AGAAGAAGCG GGAGAAGGCC 
TATCAAGCCC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA 
GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCATACTG TGAAATCAGC 
ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT 
TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGATG TGCTCTGTCT TACCATGTTT 
GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA 
ATTCGAACAG AACAGGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC 
CCCACTGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011995294.1
CDS	1940..6982
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQSLPPVAPIATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTTSLIQPLSIPYSSSTLPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKCGNVDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGFGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_012139904.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon intersectin 2 (ITSN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012139904.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCAG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTTGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGGTG TGCTGATCTC 
CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCATGAGCTG 
ATTGAGACGG AGGAGCGCTA TGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA 
CGAATAACAG AGCCAGGTTT TCTCACTGAA GGAGAGATGG CCTTGATCTT TGTTAACTGG 
AAGGAGCTCA TCATGGCTAA CACGAAACTG CTGAAGGCAC TGCGGGTGCG GAAGAAGACC 
GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC 
ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAGCTTAATG GAGCAGCCCT GTTACAGCAG 
AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG 
TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC 
CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCACGTAGA CCACTCCTCT 
CTGAAGCAGG CCCTCGAGCG GGCAGAGGAG CTCTGTTCTC AAGTGAATGA AGGCGTTCGG 
GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTC 
GCGGAGCAAC TGGTTTTCAA CTCCCTTACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC 
AGCGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTGC ACGGGTTCCT CTTCAATGAC 
TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTTGCGGTCT CCTCAGCCTC CGAGAAGCTT 
TTCAGCTCGA AGTCCAATGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAACGAA 
GCCTTGGTGA AACTGCCCAC AGACCCTTCC AGCGATGAGC CTGTCTTCCA CATCTCTCAC 
ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACAGC CTGGGTCCAG 
AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACGGAAA AGAAGAAGCG GGAGAAGGCC 
TATCAAGCCC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA 
GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCATACTG TGAAATCAGC 
ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT 
TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGATG TGCTCTGTCT TACCATGTTT 
GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA 
ATTCGAACAG AACAGGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC 
CCCACTGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_027966548.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027822349.1 Ovis aries intersectin 2 (ITSN2), transcript variant X1, mRNA.

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CloneID	OSm76894
Clone ID Related Accession (Same CDS sequence)	XM_027966548.1 , XM_012165623.3
Accession Version	XM_027966548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCCGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAGCAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCAG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTGGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGGTG TGCTGATCTC 
CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCATGAGCTG 
ATTGAGACGG AGGAGCGCTA TGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA 
CGAATAACAG AGCCAGGTTT TCTCACTGAA GGAGAGATGG CCTTGATCTT TGTTAACTGG 
AAGGAGCTCA TCATGGCTAA CACGAAACTG CTGAAGGCAC TGCGGGTGCG GAAGAAGACC 
GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC 
ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAACTTAATG GAGCAGCCCT GTTACAGCAG 
AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG 
TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC 
CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCACGTAGA CCACTCCTCT 
CTGAAGCAGG CCCTCGAGCG GGCAGAGGAG CTCTGTTCTC AAGTGAATGA AGGCGTTCGG 
GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTC 
GCGGAGCAAC TGGTTTTCAA CTCCCTTACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC 
AGCGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTGC ACGGGTTCCT CTTCAATGAC 
TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTTGCGGTCT CCTCAGCCTC CGAGAAGCTT 
TTCAGCTCGA AGTCCAATGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAACGAA 
GCCTTGGTGA AACTGCCCAC AGACCCTTCC AGCGATGAGC CTGTCTTCCA CATCTCTCAC 
ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACAGC CTGGGTCCAG 
AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACGGAAA AGAAGAAGCG GGAGAAGGCC 
TATCAAGCCC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA 
GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCATACTG TGAAATCAGC 
ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT 
TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGATG TGCTCTGTCT TACCATGTTT 
GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA 
ATTCGAACAG AACAGGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC 
CCCACTGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027822349.1
CDS	130..5172
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQSLPPVAPIATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTTSLIQPLSIPYSSSTLPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKCGNVDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGLGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_027966548.1
Organism	Ovis aries(sheep)
Definition	Ovis aries intersectin 2 (ITSN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966548.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCCGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAGCAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCAG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTGGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGGTG TGCTGATCTC 
CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCATGAGCTG 
ATTGAGACGG AGGAGCGCTA TGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA 
CGAATAACAG AGCCAGGTTT TCTCACTGAA GGAGAGATGG CCTTGATCTT TGTTAACTGG 
AAGGAGCTCA TCATGGCTAA CACGAAACTG CTGAAGGCAC TGCGGGTGCG GAAGAAGACC 
GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC 
ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAACTTAATG GAGCAGCCCT GTTACAGCAG 
AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG 
TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC 
CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCACGTAGA CCACTCCTCT 
CTGAAGCAGG CCCTCGAGCG GGCAGAGGAG CTCTGTTCTC AAGTGAATGA AGGCGTTCGG 
GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTC 
GCGGAGCAAC TGGTTTTCAA CTCCCTTACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC 
AGCGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTGC ACGGGTTCCT CTTCAATGAC 
TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTTGCGGTCT CCTCAGCCTC CGAGAAGCTT 
TTCAGCTCGA AGTCCAATGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAACGAA 
GCCTTGGTGA AACTGCCCAC AGACCCTTCC AGCGATGAGC CTGTCTTCCA CATCTCTCAC 
ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACAGC CTGGGTCCAG 
AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACGGAAA AGAAGAAGCG GGAGAAGGCC 
TATCAAGCCC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA 
GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCATACTG TGAAATCAGC 
ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT 
TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGATG TGCTCTGTCT TACCATGTTT 
GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA 
ATTCGAACAG AACAGGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC 
CCCACTGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_012165623.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012021013.2 Ovis aries intersectin 2 (ITSN2), transcript variant X2, mRNA.

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CloneID	OSm76894
Clone ID Related Accession (Same CDS sequence)	XM_027966548.1 , XM_012165623.3
Accession Version	XM_012165623.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_012165623.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCCGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAGCAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCAG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTGGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGGTG TGCTGATCTC 
CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCATGAGCTG 
ATTGAGACGG AGGAGCGCTA TGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA 
CGAATAACAG AGCCAGGTTT TCTCACTGAA GGAGAGATGG CCTTGATCTT TGTTAACTGG 
AAGGAGCTCA TCATGGCTAA CACGAAACTG CTGAAGGCAC TGCGGGTGCG GAAGAAGACC 
GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC 
ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAACTTAATG GAGCAGCCCT GTTACAGCAG 
AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG 
TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC 
CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCACGTAGA CCACTCCTCT 
CTGAAGCAGG CCCTCGAGCG GGCAGAGGAG CTCTGTTCTC AAGTGAATGA AGGCGTTCGG 
GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTC 
GCGGAGCAAC TGGTTTTCAA CTCCCTTACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC 
AGCGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTGC ACGGGTTCCT CTTCAATGAC 
TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTTGCGGTCT CCTCAGCCTC CGAGAAGCTT 
TTCAGCTCGA AGTCCAATGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAACGAA 
GCCTTGGTGA AACTGCCCAC AGACCCTTCC AGCGATGAGC CTGTCTTCCA CATCTCTCAC 
ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACAGC CTGGGTCCAG 
AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACGGAAA AGAAGAAGCG GGAGAAGGCC 
TATCAAGCCC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA 
GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCATACTG TGAAATCAGC 
ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT 
TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGATG TGCTCTGTCT TACCATGTTT 
GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA 
ATTCGAACAG AACAGGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC 
CCCACTGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012021013.2
CDS	57..5099
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQSLPPVAPIATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTTSLIQPLSIPYSSSTLPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKCGNVDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGLGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_012165623.3
Organism	Ovis aries(sheep)
Definition	Ovis aries intersectin 2 (ITSN2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012165623.3

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCCGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAGCAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCAG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTGGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGGTG TGCTGATCTC 
CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCATGAGCTG 
ATTGAGACGG AGGAGCGCTA TGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA 
CGAATAACAG AGCCAGGTTT TCTCACTGAA GGAGAGATGG CCTTGATCTT TGTTAACTGG 
AAGGAGCTCA TCATGGCTAA CACGAAACTG CTGAAGGCAC TGCGGGTGCG GAAGAAGACC 
GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC 
ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAACTTAATG GAGCAGCCCT GTTACAGCAG 
AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG 
TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC 
CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCACGTAGA CCACTCCTCT 
CTGAAGCAGG CCCTCGAGCG GGCAGAGGAG CTCTGTTCTC AAGTGAATGA AGGCGTTCGG 
GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTC 
GCGGAGCAAC TGGTTTTCAA CTCCCTTACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC 
AGCGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTGC ACGGGTTCCT CTTCAATGAC 
TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTTGCGGTCT CCTCAGCCTC CGAGAAGCTT 
TTCAGCTCGA AGTCCAATGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAACGAA 
GCCTTGGTGA AACTGCCCAC AGACCCTTCC AGCGATGAGC CTGTCTTCCA CATCTCTCAC 
ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACAGC CTGGGTCCAG 
AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACGGAAA AGAAGAAGCG GGAGAAGGCC 
TATCAAGCCC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA 
GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCATACTG TGAAATCAGC 
ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT 
TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGATG TGCTCTGTCT TACCATGTTT 
GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA 
ATTCGAACAG AACAGGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC 
CCCACTGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_027966549.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027822350.1 Ovis aries intersectin 2 (ITSN2), transcript variant X3, mRNA.

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CloneID	OSm76895
Clone ID Related Accession (Same CDS sequence)	XM_027966549.1
Accession Version	XM_027966549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	intersectin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCCGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAGCAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG 
ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG 
AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA 
AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA 
AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG 
GTGAATTACA GAGCATTGTA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT 
TCTGGGGATA TAATACAGGT TGACGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT 
GGTTTTCAAG GAAATTTTGG CTGGTTTCCA TGCAATTATG TAGAAAAAGT GACATCAAGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT 
TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGACTG ATTATCAAAA TGTATCTTTT 
TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC 
CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TGGAAAACCT GAAAGCACAG 
GCCCTTTGTT CTTGGACAGC AAAGAAGGAA AACCACTTGA ACTTCTCAAA ACATGATATC 
ATTACTGTTT TGGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA 
TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA 
GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTACCTCTG CAGCCTGTAC AGTGGGAGAA 
GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA 
GGTGAAGAAC TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC 
AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTGGGGAGT 
GCTAGCAAAT CTGGAACATC AAACAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT 
GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG 
AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA 
GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTACACCT 
GCCTTTCATC CTGTATGTCA GGTGATTGCC ATGTATGACT ACACAGCAAA CAACGAGGAT 
GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTTTAA ACAAGGATGA TCCTGACTGG 
TGGCAAGGAG AAATCAGTGG GGTGACTGGT CTCTTCCCTT CGAACTATGT TAAGATGACG 
ACGGATGCAG ATCCAAGTCA GCAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG 
CCGGTGGAAA GGAAGAGGCA GGGCTACATC CATGAGCTGA TTGAGACGGA GGAGCGCTAT 
GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT 
CTCACTGAAG GAGAGATGGC CTTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC 
ACGAAACTGC TGAAGGCACT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG 
CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC 
TGCAGCTGTC AACTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC 
TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC 
AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT 
CTGGAGAACA CCCCAGAGAA TCACGTAGAC CACTCCTCTC TGAAGCAGGC CCTCGAGCGG 
GCAGAGGAGC TCTGTTCTCA AGTGAATGAA GGCGTTCGGG AGAAGGAAAA TTCAGACCGA 
CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTCG CGGAGCAACT GGTTTTCAAC 
TCCCTTACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GCGGAAAGCT GTACAAGACC 
AAGAGCAACA AGGAGCTGCA CGGGTTCCTC TTCAATGACT TCCTGCTTCT CACCTACATG 
GTCAAGCAGT TTGCGGTCTC CTCAGCCTCC GAGAAGCTTT TCAGCTCGAA GTCCAATGCT 
CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAACGAAG CCTTGGTGAA ACTGCCCACA 
GACCCTTCCA GCGATGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC 
CGAACAGACA ACATTAACGA GAGGACAGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG 
CAGTACATCG ACACGGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCCCG CTCTCAGAAG 
TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT 
AAACCAAATG GAAAGAGCAA TCCATACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC 
ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT 
AAGGACCTCT ATCAGGATGT GCTCTGTCTT ACCATGTTTG ACAGAGATCA GTTTTCACCA 
GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAGGAAAGC 
AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACTGGGGA GGTCTGGGTC 
CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027822350.1
CDS	127..5088
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQSLPPVAPIATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTTSLIQPLSIPYSSSTLPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGLGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_027966549.1
Organism	Ovis aries(sheep)
Definition	Ovis aries intersectin 2 (ITSN2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966549.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCCGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAGCAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG 
ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG 
AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA 
AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA 
AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCAGC TGGTACTTTG 
GTGAATTACA GAGCATTGTA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT 
TCTGGGGATA TAATACAGGT TGACGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT 
GGTTTTCAAG GAAATTTTGG CTGGTTTCCA TGCAATTATG TAGAAAAAGT GACATCAAGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT 
TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGACTG ATTATCAAAA TGTATCTTTT 
TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC 
CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TGGAAAACCT GAAAGCACAG 
GCCCTTTGTT CTTGGACAGC AAAGAAGGAA AACCACTTGA ACTTCTCAAA ACATGATATC 
ATTACTGTTT TGGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA 
TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA 
GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTACCTCTG CAGCCTGTAC AGTGGGAGAA 
GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA 
GGTGAAGAAC TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC 
AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTGGGGAGT 
GCTAGCAAAT CTGGAACATC AAACAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT 
GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG 
AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA 
GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTACACCT 
GCCTTTCATC CTGTATGTCA GGTGATTGCC ATGTATGACT ACACAGCAAA CAACGAGGAT 
GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTTTAA ACAAGGATGA TCCTGACTGG 
TGGCAAGGAG AAATCAGTGG GGTGACTGGT CTCTTCCCTT CGAACTATGT TAAGATGACG 
ACGGATGCAG ATCCAAGTCA GCAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG 
CCGGTGGAAA GGAAGAGGCA GGGCTACATC CATGAGCTGA TTGAGACGGA GGAGCGCTAT 
GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT 
CTCACTGAAG GAGAGATGGC CTTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC 
ACGAAACTGC TGAAGGCACT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG 
CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC 
TGCAGCTGTC AACTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC 
TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC 
AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT 
CTGGAGAACA CCCCAGAGAA TCACGTAGAC CACTCCTCTC TGAAGCAGGC CCTCGAGCGG 
GCAGAGGAGC TCTGTTCTCA AGTGAATGAA GGCGTTCGGG AGAAGGAAAA TTCAGACCGA 
CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTCG CGGAGCAACT GGTTTTCAAC 
TCCCTTACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GCGGAAAGCT GTACAAGACC 
AAGAGCAACA AGGAGCTGCA CGGGTTCCTC TTCAATGACT TCCTGCTTCT CACCTACATG 
GTCAAGCAGT TTGCGGTCTC CTCAGCCTCC GAGAAGCTTT TCAGCTCGAA GTCCAATGCT 
CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAACGAAG CCTTGGTGAA ACTGCCCACA 
GACCCTTCCA GCGATGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC 
CGAACAGACA ACATTAACGA GAGGACAGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG 
CAGTACATCG ACACGGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCCCG CTCTCAGAAG 
TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT 
AAACCAAATG GAAAGAGCAA TCCATACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC 
ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT 
AAGGACCTCT ATCAGGATGT GCTCTGTCTT ACCATGTTTG ACAGAGATCA GTTTTCACCA 
GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAGGAAAGC 
AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACTGGGGA GGTCTGGGTC 
CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_012165614.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012021004.1 Ovis aries intersectin 2 (ITSN2), transcript variant X2, mRNA.

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CloneID	OSm29488
Clone ID Related Accession (Same CDS sequence)	XM_012165614.2 , XM_012165618.2 , XM_012165623.2 , XM_012165629.2
Accession Version	XM_012165614.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	intersectin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012165614.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCGG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTTGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGGTG TGCTGATCTC 
CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCATGAGCTG 
ATTGAGACGG AGGAGCGCTA TGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA 
CGAATAACAG AGCCAGGTTT TCTCACTGAA GGAGAGATGG CCTTGATCTT TGTTAACTGG 
AAGGAGCTCA TCATGGCTAA CACGAAACTG CTGAAGGCAC TGCGGGTGCG GAAGAAGACC 
GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC 
ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAACTTAATG GAGCAGCCCT GTTACAGCAG 
AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG 
TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC 
CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCACGTAGA CCACTCCTCT 
CTGAAGCAGG CCCTCGAGCG GGCAGAGGAG CTCTGTTCTC AAGTGAATGA AGGCGTTCGG 
GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTC 
GCGGAGCAAC TGGTTTTCAA CTCCCTTACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC 
AGCGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTGC ACGGGTTCCT CTTCAATGAC 
TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTTGCGGTCT CCTCAGCCTC CGAGAAGCTT 
TTCAGCTCGA AGTCCAATGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAACGAA 
GCCTTGGTGA AACTGCCCAC AGACCCTTCC AGCGATGAGC CTGTCTTCCA CATCTCTCAC 
ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACAGC CTGGGTCCAG 
AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACGGAAA AGAAGAAGCG GGAGAAGGCC 
TATCAAGCCC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA 
GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCATACTG TGAAATCAGC 
ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT 
TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGATG TGCTCTGTCT TACCATGTTT 
GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA 
ATTCGAACAG AACAGGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC 
CCCACTGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012021004.1
CDS	1851..6893
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQSLPPVAPIATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTTSLIQPLSIPYSSSTLPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKCGNVDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGFGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_012165614.2
Organism	Ovis aries(sheep)
Definition	Ovis aries intersectin 2 (ITSN2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012165614.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCGG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTTGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGGTG TGCTGATCTC 
CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCATGAGCTG 
ATTGAGACGG AGGAGCGCTA TGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA 
CGAATAACAG AGCCAGGTTT TCTCACTGAA GGAGAGATGG CCTTGATCTT TGTTAACTGG 
AAGGAGCTCA TCATGGCTAA CACGAAACTG CTGAAGGCAC TGCGGGTGCG GAAGAAGACC 
GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC 
ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAACTTAATG GAGCAGCCCT GTTACAGCAG 
AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG 
TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC 
CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCACGTAGA CCACTCCTCT 
CTGAAGCAGG CCCTCGAGCG GGCAGAGGAG CTCTGTTCTC AAGTGAATGA AGGCGTTCGG 
GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTC 
GCGGAGCAAC TGGTTTTCAA CTCCCTTACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC 
AGCGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTGC ACGGGTTCCT CTTCAATGAC 
TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTTGCGGTCT CCTCAGCCTC CGAGAAGCTT 
TTCAGCTCGA AGTCCAATGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAACGAA 
GCCTTGGTGA AACTGCCCAC AGACCCTTCC AGCGATGAGC CTGTCTTCCA CATCTCTCAC 
ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACAGC CTGGGTCCAG 
AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACGGAAA AGAAGAAGCG GGAGAAGGCC 
TATCAAGCCC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA 
GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCATACTG TGAAATCAGC 
ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT 
TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGATG TGCTCTGTCT TACCATGTTT 
GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA 
ATTCGAACAG AACAGGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC 
CCCACTGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_012165618.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012021008.1 Ovis aries intersectin 2 (ITSN2), transcript variant X1, mRNA.

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CloneID	OSm29488
Clone ID Related Accession (Same CDS sequence)	XM_012165614.2 , XM_012165618.2 , XM_012165623.2 , XM_012165629.2
Accession Version	XM_012165618.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	intersectin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012165618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCGG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTTGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGGTG TGCTGATCTC 
CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCATGAGCTG 
ATTGAGACGG AGGAGCGCTA TGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA 
CGAATAACAG AGCCAGGTTT TCTCACTGAA GGAGAGATGG CCTTGATCTT TGTTAACTGG 
AAGGAGCTCA TCATGGCTAA CACGAAACTG CTGAAGGCAC TGCGGGTGCG GAAGAAGACC 
GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC 
ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAACTTAATG GAGCAGCCCT GTTACAGCAG 
AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG 
TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC 
CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCACGTAGA CCACTCCTCT 
CTGAAGCAGG CCCTCGAGCG GGCAGAGGAG CTCTGTTCTC AAGTGAATGA AGGCGTTCGG 
GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTC 
GCGGAGCAAC TGGTTTTCAA CTCCCTTACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC 
AGCGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTGC ACGGGTTCCT CTTCAATGAC 
TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTTGCGGTCT CCTCAGCCTC CGAGAAGCTT 
TTCAGCTCGA AGTCCAATGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAACGAA 
GCCTTGGTGA AACTGCCCAC AGACCCTTCC AGCGATGAGC CTGTCTTCCA CATCTCTCAC 
ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACAGC CTGGGTCCAG 
AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACGGAAA AGAAGAAGCG GGAGAAGGCC 
TATCAAGCCC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA 
GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCATACTG TGAAATCAGC 
ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT 
TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGATG TGCTCTGTCT TACCATGTTT 
GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA 
ATTCGAACAG AACAGGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC 
CCCACTGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012021008.1
CDS	1940..6982
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQSLPPVAPIATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTTSLIQPLSIPYSSSTLPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKCGNVDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGFGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_012165618.2
Organism	Ovis aries(sheep)
Definition	Ovis aries intersectin 2 (ITSN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012165618.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCGG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTTGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGGTG TGCTGATCTC 
CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCATGAGCTG 
ATTGAGACGG AGGAGCGCTA TGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA 
CGAATAACAG AGCCAGGTTT TCTCACTGAA GGAGAGATGG CCTTGATCTT TGTTAACTGG 
AAGGAGCTCA TCATGGCTAA CACGAAACTG CTGAAGGCAC TGCGGGTGCG GAAGAAGACC 
GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC 
ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAACTTAATG GAGCAGCCCT GTTACAGCAG 
AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG 
TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC 
CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCACGTAGA CCACTCCTCT 
CTGAAGCAGG CCCTCGAGCG GGCAGAGGAG CTCTGTTCTC AAGTGAATGA AGGCGTTCGG 
GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTC 
GCGGAGCAAC TGGTTTTCAA CTCCCTTACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC 
AGCGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTGC ACGGGTTCCT CTTCAATGAC 
TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTTGCGGTCT CCTCAGCCTC CGAGAAGCTT 
TTCAGCTCGA AGTCCAATGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAACGAA 
GCCTTGGTGA AACTGCCCAC AGACCCTTCC AGCGATGAGC CTGTCTTCCA CATCTCTCAC 
ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACAGC CTGGGTCCAG 
AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACGGAAA AGAAGAAGCG GGAGAAGGCC 
TATCAAGCCC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA 
GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCATACTG TGAAATCAGC 
ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT 
TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGATG TGCTCTGTCT TACCATGTTT 
GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA 
ATTCGAACAG AACAGGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC 
CCCACTGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_012165623.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012021013.1 Ovis aries intersectin 2 (ITSN2), transcript variant X3, mRNA.

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CloneID	OSm29488
Clone ID Related Accession (Same CDS sequence)	XM_012165614.2 , XM_012165618.2 , XM_012165623.2 , XM_012165629.2
Accession Version	XM_012165623.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	intersectin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012165623.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCGG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTTGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGGTG TGCTGATCTC 
CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCATGAGCTG 
ATTGAGACGG AGGAGCGCTA TGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA 
CGAATAACAG AGCCAGGTTT TCTCACTGAA GGAGAGATGG CCTTGATCTT TGTTAACTGG 
AAGGAGCTCA TCATGGCTAA CACGAAACTG CTGAAGGCAC TGCGGGTGCG GAAGAAGACC 
GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC 
ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAACTTAATG GAGCAGCCCT GTTACAGCAG 
AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG 
TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC 
CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCACGTAGA CCACTCCTCT 
CTGAAGCAGG CCCTCGAGCG GGCAGAGGAG CTCTGTTCTC AAGTGAATGA AGGCGTTCGG 
GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTC 
GCGGAGCAAC TGGTTTTCAA CTCCCTTACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC 
AGCGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTGC ACGGGTTCCT CTTCAATGAC 
TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTTGCGGTCT CCTCAGCCTC CGAGAAGCTT 
TTCAGCTCGA AGTCCAATGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAACGAA 
GCCTTGGTGA AACTGCCCAC AGACCCTTCC AGCGATGAGC CTGTCTTCCA CATCTCTCAC 
ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACAGC CTGGGTCCAG 
AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACGGAAA AGAAGAAGCG GGAGAAGGCC 
TATCAAGCCC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA 
GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCATACTG TGAAATCAGC 
ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT 
TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGATG TGCTCTGTCT TACCATGTTT 
GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA 
ATTCGAACAG AACAGGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC 
CCCACTGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012021013.1
CDS	84..5126
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQSLPPVAPIATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTTSLIQPLSIPYSSSTLPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKCGNVDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGFGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_012165623.2
Organism	Ovis aries(sheep)
Definition	Ovis aries intersectin 2 (ITSN2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012165623.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCGG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTTGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGGTG TGCTGATCTC 
CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCATGAGCTG 
ATTGAGACGG AGGAGCGCTA TGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA 
CGAATAACAG AGCCAGGTTT TCTCACTGAA GGAGAGATGG CCTTGATCTT TGTTAACTGG 
AAGGAGCTCA TCATGGCTAA CACGAAACTG CTGAAGGCAC TGCGGGTGCG GAAGAAGACC 
GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC 
ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAACTTAATG GAGCAGCCCT GTTACAGCAG 
AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG 
TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC 
CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCACGTAGA CCACTCCTCT 
CTGAAGCAGG CCCTCGAGCG GGCAGAGGAG CTCTGTTCTC AAGTGAATGA AGGCGTTCGG 
GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTC 
GCGGAGCAAC TGGTTTTCAA CTCCCTTACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC 
AGCGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTGC ACGGGTTCCT CTTCAATGAC 
TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTTGCGGTCT CCTCAGCCTC CGAGAAGCTT 
TTCAGCTCGA AGTCCAATGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAACGAA 
GCCTTGGTGA AACTGCCCAC AGACCCTTCC AGCGATGAGC CTGTCTTCCA CATCTCTCAC 
ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACAGC CTGGGTCCAG 
AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACGGAAA AGAAGAAGCG GGAGAAGGCC 
TATCAAGCCC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA 
GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCATACTG TGAAATCAGC 
ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT 
TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGATG TGCTCTGTCT TACCATGTTT 
GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA 
ATTCGAACAG AACAGGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC 
CCCACTGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_012165629.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012021019.1 Ovis aries intersectin 2 (ITSN2), transcript variant X4, mRNA.

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CloneID	OSm29488
Clone ID Related Accession (Same CDS sequence)	XM_012165614.2 , XM_012165618.2 , XM_012165623.2 , XM_012165629.2
Accession Version	XM_012165629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	intersectin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012165629.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCGG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTTGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGGTG TGCTGATCTC 
CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCATGAGCTG 
ATTGAGACGG AGGAGCGCTA TGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA 
CGAATAACAG AGCCAGGTTT TCTCACTGAA GGAGAGATGG CCTTGATCTT TGTTAACTGG 
AAGGAGCTCA TCATGGCTAA CACGAAACTG CTGAAGGCAC TGCGGGTGCG GAAGAAGACC 
GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC 
ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAACTTAATG GAGCAGCCCT GTTACAGCAG 
AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG 
TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC 
CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCACGTAGA CCACTCCTCT 
CTGAAGCAGG CCCTCGAGCG GGCAGAGGAG CTCTGTTCTC AAGTGAATGA AGGCGTTCGG 
GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTC 
GCGGAGCAAC TGGTTTTCAA CTCCCTTACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC 
AGCGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTGC ACGGGTTCCT CTTCAATGAC 
TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTTGCGGTCT CCTCAGCCTC CGAGAAGCTT 
TTCAGCTCGA AGTCCAATGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAACGAA 
GCCTTGGTGA AACTGCCCAC AGACCCTTCC AGCGATGAGC CTGTCTTCCA CATCTCTCAC 
ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACAGC CTGGGTCCAG 
AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACGGAAA AGAAGAAGCG GGAGAAGGCC 
TATCAAGCCC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA 
GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCATACTG TGAAATCAGC 
ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT 
TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGATG TGCTCTGTCT TACCATGTTT 
GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA 
ATTCGAACAG AACAGGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC 
CCCACTGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012021019.1
CDS	89..5131
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQSLPPVAPIATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTTSLIQPLSIPYSSSTLPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKCGNVDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGFGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_012165629.2
Organism	Ovis aries(sheep)
Definition	Ovis aries intersectin 2 (ITSN2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012165629.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGTGTG GGAATGTGGA TGACTCTGTT CTTCAGTGCC TTTTGTCTCT GCTAAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTGAGAGAAA GCTACAATAC ACAGCAGTTA 
GCCATTGAAC AGCTTTATAA GATCAAACGT GACAAACTGA GGGAAATTGA AAGAAAAAGA 
TCAGAGTTAA TACAGAAAAA GAAACTAGAA GATGAGGCTA CAAGGAAAGC AAAACAAGGA 
AAAGAAAACT TATGGAAAGA AAATCTTAGA AAGGAGGAAG AAGAAAAACA AAAACGACTC 
CAAGAAGAAA AAGCACAGGA AAAAGTTCAA GAAGTGGAGC GGAAAGCTGA GGAGAAACAA 
AGTGAGCCGG CTGGTACTTT GGTGAATTAC AGAGCATTGT ATCGCTTTGA AGCAAGGAAC 
CATGATGAAA TGAGTTTTAA TTCTGGGGAT ATAATACAGG TTGACGAAAA AACTGTAGGA 
GAACCTGGTT GGCTTTATGG TGGTTTTCAA GGAAATTTTG GCTGGTTTCC ATGCAATTAT 
GTAGAAAAAG TGACATCAAG TGAAAAATCT GTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTATCTG CTACCTCAAC TTCTGAATCA CTTTCTTCAA ACCAACCAGC ATCTGTGACT 
GATTATCAAA ATGTATCTTT TTCAAACCTA AGTGTTAACA CATCATGGCA GAAAAAATCA 
GCTTTTACTC GCACTGTGTC CCCTGTGTCT GTATCACCGA TTCATGGACA GGGACAGGTT 
GTGGAAAACC TGAAAGCACA GGCCCTTTGT TCTTGGACAG CAAAGAAGGA AAACCACTTG 
AACTTCTCAA AACATGATAT CATTACTGTT TTGGAGCAGC AAGAAAATTG GTGGTTTGGG 
GAGGTTCACG GAGGAAGAGG ATGGTTTCCG AAATCTTACG TCAAAATCAT CCCTGGGAGT 
GAAGTTAAGC GGGAAGAACC AGAAGCTTTG TATGCATCTG TAAACAAGAA ACCTACCTCT 
GCAGCCTGTA CAGTGGGAGA AGAGTATATT GCACTTTATT CATATTCAAG TGTAGAACCT 
GGAGATTTGA CTTTCACTGA AGGTGAAGAA CTATTGGTGA CCCAGAAAGA TGGAGAATGG 
TGGACAGGAA GTATTGGAGA CAGAACTGGA ATTTTTCCAT CAAATTATGT CAAACCAAAA 
GATCAGGAGG GTTTTGGGAG TGCTAGCAAA TCTGGAACAT CAAACAAGAA ACCTGAGATT 
GCTCAAGTAA CATCAGCATA TGCGGCTTCT GGTTCTGAAC AGCTTAGCCT CGCACCAGGA 
CAGTTAATAC TAATTCTAAA GAAAAACACA AGTGGGTGGT GGCAAGGGGA ATTACAGGCC 
AGAGGGAAAA AGCGACAGAA AGGATGGTTT CCTGCCAGCC ATGTTAAACT TTTGGGTCCA 
AGTAGTGAAA GAACTACACC TGCCTTTCAT CCTGTATGTC AGGTGATTGC CATGTATGAC 
TACACAGCAA ACAACGAGGA TGAGCTCAGT TTCTCCAAGG GACAGCTTAT TAATGTTTTA 
AACAAGGATG ATCCTGACTG GTGGCAAGGA GAAATCAGTG GGGTGACTGG TCTCTTCCCT 
TCGAACTATG TTAAGATGAC GACGGATGCA GATCCAAGTC AGCAGTGGTG TGCTGATCTC 
CAAACACTGG ACACCATGCA GCCGGTGGAA AGGAAGAGGC AGGGCTACAT CCATGAGCTG 
ATTGAGACGG AGGAGCGCTA TGTGGACGAC CTGCAGCTCG TCGTGGAGGT TTTTCAGAAA 
CGAATAACAG AGCCAGGTTT TCTCACTGAA GGAGAGATGG CCTTGATCTT TGTTAACTGG 
AAGGAGCTCA TCATGGCTAA CACGAAACTG CTGAAGGCAC TGCGGGTGCG GAAGAAGACC 
GGGGGTGAGA AGATGCCCGT GCACATGATC GGGGACATCC TGGCCGCTGA GCTGTCCCAC 
ATGCAGGCCT ACATCCGCTT CTGCAGCTGT CAACTTAATG GAGCAGCCCT GTTACAGCAG 
AAGACAGACG AACACGCAGA CTTCAAAGAA TTTTTAAAGA AGTTGGCATC TGACCCTCGG 
TGTAAAGGGA TGCCCCTTTC CAGCTTCCTG CTGAAGCCCA TGCAGAGGAT CACCCGCTAC 
CCACTCCTCA TCAGAAGCAT TCTGGAGAAC ACCCCAGAGA ATCACGTAGA CCACTCCTCT 
CTGAAGCAGG CCCTCGAGCG GGCAGAGGAG CTCTGTTCTC AAGTGAATGA AGGCGTTCGG 
GAGAAGGAAA ATTCAGACCG ACTGGAGTGG ATCCAGACGC ACGTGCAGTG CGATGGGCTC 
GCGGAGCAAC TGGTTTTCAA CTCCCTTACC AACTGCCTGG GGCCCCGGAA GCTCCTGCAC 
AGCGGAAAGC TGTACAAGAC CAAGAGCAAC AAGGAGCTGC ACGGGTTCCT CTTCAATGAC 
TTCCTGCTTC TCACCTACAT GGTCAAGCAG TTTGCGGTCT CCTCAGCCTC CGAGAAGCTT 
TTCAGCTCGA AGTCCAATGC TCAGTTCAAG ATGTATAAGA CGCCCATTTT CCTGAACGAA 
GCCTTGGTGA AACTGCCCAC AGACCCTTCC AGCGATGAGC CTGTCTTCCA CATCTCTCAC 
ATTGACCGGG TCTACACCCT CCGAACAGAC AACATTAACG AGAGGACAGC CTGGGTCCAG 
AAGATCAAGG CCGCGTCTGA GCAGTACATC GACACGGAAA AGAAGAAGCG GGAGAAGGCC 
TATCAAGCCC GCTCTCAGAA GTCTTCAGGC ATCGGGCGTC TGATGGTGCA TGTCATTGAA 
GCTACAGAAC TGAAAGCCTG TAAACCAAAT GGAAAGAGCA ATCCATACTG TGAAATCAGC 
ATGGGCTCCC AGAGCTACAC CACCAGAACC CTCCAGGACA CACTAAATCC CAAATGGAAT 
TTTAACTGCC AGTTCTTCAT TAAGGACCTC TATCAGGATG TGCTCTGTCT TACCATGTTT 
GACAGAGATC AGTTTTCACC AGACGATTTC CTGGGTCGCA CTGAAGTTCC AGTGGCAAAA 
ATTCGAACAG AACAGGAAAG CAAAGGCCCT ACAACCCGCC GACTCCTGCT GCACGAGGTC 
CCCACTGGGG AGGTCTGGGT CCGCTTTGAC CTGCAGCTTT TTGAGCAGAA AACTCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_012165644.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012021034.1 Ovis aries intersectin 2 (ITSN2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSm29489
Clone ID Related Accession (Same CDS sequence)	XM_012165644.2
Accession Version	XM_012165644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012165644.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG 
ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG 
AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA 
AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA 
AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCGGC TGGTACTTTG 
GTGAATTACA GAGCATTGTA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT 
TCTGGGGATA TAATACAGGT TGACGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT 
GGTTTTCAAG GAAATTTTGG CTGGTTTCCA TGCAATTATG TAGAAAAAGT GACATCAAGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT 
TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGACTG ATTATCAAAA TGTATCTTTT 
TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC 
CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TGGAAAACCT GAAAGCACAG 
GCCCTTTGTT CTTGGACAGC AAAGAAGGAA AACCACTTGA ACTTCTCAAA ACATGATATC 
ATTACTGTTT TGGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA 
TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA 
GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTACCTCTG CAGCCTGTAC AGTGGGAGAA 
GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA 
GGTGAAGAAC TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC 
AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT 
GCTAGCAAAT CTGGAACATC AAACAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT 
GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG 
AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA 
GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTACACCT 
GCCTTTCATC CTGTATGTCA GGTGATTGCC ATGTATGACT ACACAGCAAA CAACGAGGAT 
GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTTTAA ACAAGGATGA TCCTGACTGG 
TGGCAAGGAG AAATCAGTGG GGTGACTGGT CTCTTCCCTT CGAACTATGT TAAGATGACG 
ACGGATGCAG ATCCAAGTCA GCAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG 
CCGGTGGAAA GGAAGAGGCA GGGCTACATC CATGAGCTGA TTGAGACGGA GGAGCGCTAT 
GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT 
CTCACTGAAG GAGAGATGGC CTTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC 
ACGAAACTGC TGAAGGCACT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG 
CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC 
TGCAGCTGTC AACTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC 
TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC 
AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT 
CTGGAGAACA CCCCAGAGAA TCACGTAGAC CACTCCTCTC TGAAGCAGGC CCTCGAGCGG 
GCAGAGGAGC TCTGTTCTCA AGTGAATGAA GGCGTTCGGG AGAAGGAAAA TTCAGACCGA 
CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTCG CGGAGCAACT GGTTTTCAAC 
TCCCTTACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GCGGAAAGCT GTACAAGACC 
AAGAGCAACA AGGAGCTGCA CGGGTTCCTC TTCAATGACT TCCTGCTTCT CACCTACATG 
GTCAAGCAGT TTGCGGTCTC CTCAGCCTCC GAGAAGCTTT TCAGCTCGAA GTCCAATGCT 
CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAACGAAG CCTTGGTGAA ACTGCCCACA 
GACCCTTCCA GCGATGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC 
CGAACAGACA ACATTAACGA GAGGACAGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG 
CAGTACATCG ACACGGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCCCG CTCTCAGAAG 
TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT 
AAACCAAATG GAAAGAGCAA TCCATACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC 
ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT 
AAGGACCTCT ATCAGGATGT GCTCTGTCTT ACCATGTTTG ACAGAGATCA GTTTTCACCA 
GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAGGAAAGC 
AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACTGGGGA GGTCTGGGTC 
CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012021034.1
CDS	1851..6812
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQSLPPVAPIATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTTSLIQPLSIPYSSSTLPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGFGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_012165644.2
Organism	Ovis aries(sheep)
Definition	Ovis aries intersectin 2 (ITSN2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012165644.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGGAATGGGA 
AGCATGCCCA ATCTGTCCAT TCCTCAGTCA TTGCCTCCAG TTGCACCCAT AGCAACACCC 
TTGTCCTCGG CGACTTCAGG GACCACCCTC CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GCACTTCATC ATTACCAAAC GGAACCACCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT ACGTCATCTT ACAGTCTGAT GATGGGAGGA 
TTTGGTGGTG CTAATATACA GAAAGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAT TCACCCAAGA CTGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAATATCGG CAAAAATTTA ACAGTCTTGA CAAAAGCATG 
AGTGGATACC TCTCAGGTTT TCAAGCTAGA AATGCTCTTC TTCAGTCAAA TCTTTCTCAA 
ACTCAGCTAG CTACTATATG GACTCTGGCT GACATTGATG GTGATGGACA GCTGAAAGCT 
GAGGAATTCA TCCTTGCAAT GCACCTCACT GATATGGCCA AAGCTGGACA GCCATTGCCA 
CTGGCTTTAC CTCCTGAGCT TGTCCCTCCA TCTTTTAGAG GAGGAAAGCA GATTGATTCC 
ATTAACGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAACCTCA GAAAAAATTA 
CCAGTTACGT TCGAGGACAA GCGGAAAGCC AACTATGAGC GGGGCAACAT GGAACTGGAG 
AAGCGACGCC AGGCCATGAT GGAGCAGCAG CAGAGGGAGG CAGAGCGCAA AGCCCAGAAA 
GAAAAGGAAG AATGGGAACG AAAACAGAGA GAGCTACAAG AGCAAGAATG GAAGAAACAA 
CTTGAATTAG AAAAACGCTT GGAGAAGCAG AGGGAATTGG AGCGACAACG GGAAGAAGAA 
AGGAGAAAAG AGATAGAAAG ACGAGAGGCC GCAAAACAGG AACTTGAAAG ACAACGTCGT 
TTAGAATGGG AGAGAATTCG TAGGCAGGAG CTTCTCAGTC GAAGGAATAG AGAACAGGAA 
GAAATTGTCA GGTTAAACTC TAAAAAGAAG AGCCTCCATC TCGAGCTGGA AGCACTGAAT 
GGCAAACATC AACAGATCTC AGGCAGACTT CAAGACGTCC GACTCAGAAA GCAAACTCAG 
AAGACCGAGC TGGAAGTTCT GGATAAGCAG TGTGACCTGG AAATCATGGA AATCAAGCAG 
CTTCAACAGG AGCTTCAGGA GTATCAAAAT AAACTTATCT ATTTGGTGCC AGAGAAGCAG 
TTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTT AGGGATCAGT 
TTACTTCATA AAAAATCATT AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCCAAG CTTTCAGAAA TGGATTCATT TAACAATGAA 
CTGAAGGAAC TGAGAGAAAG CTACAATACA CAGCAGTTAG CCATTGAACA GCTTTATAAG 
ATCAAACGTG ACAAACTGAG GGAAATTGAA AGAAAAAGAT CAGAGTTAAT ACAGAAAAAG 
AAACTAGAAG ATGAGGCTAC AAGGAAAGCA AAACAAGGAA AAGAAAACTT ATGGAAAGAA 
AATCTTAGAA AGGAGGAAGA AGAAAAACAA AAACGACTCC AAGAAGAAAA AGCACAGGAA 
AAAGTTCAAG AAGTGGAGCG GAAAGCTGAG GAGAAACAAA GTGAGCCGGC TGGTACTTTG 
GTGAATTACA GAGCATTGTA TCGCTTTGAA GCAAGGAACC ATGATGAAAT GAGTTTTAAT 
TCTGGGGATA TAATACAGGT TGACGAAAAA ACTGTAGGAG AACCTGGTTG GCTTTATGGT 
GGTTTTCAAG GAAATTTTGG CTGGTTTCCA TGCAATTATG TAGAAAAAGT GACATCAAGT 
GAAAAATCTG TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTATCTGC TACCTCAACT 
TCTGAATCAC TTTCTTCAAA CCAACCAGCA TCTGTGACTG ATTATCAAAA TGTATCTTTT 
TCAAACCTAA GTGTTAACAC ATCATGGCAG AAAAAATCAG CTTTTACTCG CACTGTGTCC 
CCTGTGTCTG TATCACCGAT TCATGGACAG GGACAGGTTG TGGAAAACCT GAAAGCACAG 
GCCCTTTGTT CTTGGACAGC AAAGAAGGAA AACCACTTGA ACTTCTCAAA ACATGATATC 
ATTACTGTTT TGGAGCAGCA AGAAAATTGG TGGTTTGGGG AGGTTCACGG AGGAAGAGGA 
TGGTTTCCGA AATCTTACGT CAAAATCATC CCTGGGAGTG AAGTTAAGCG GGAAGAACCA 
GAAGCTTTGT ATGCATCTGT AAACAAGAAA CCTACCTCTG CAGCCTGTAC AGTGGGAGAA 
GAGTATATTG CACTTTATTC ATATTCAAGT GTAGAACCTG GAGATTTGAC TTTCACTGAA 
GGTGAAGAAC TATTGGTGAC CCAGAAAGAT GGAGAATGGT GGACAGGAAG TATTGGAGAC 
AGAACTGGAA TTTTTCCATC AAATTATGTC AAACCAAAAG ATCAGGAGGG TTTTGGGAGT 
GCTAGCAAAT CTGGAACATC AAACAAGAAA CCTGAGATTG CTCAAGTAAC ATCAGCATAT 
GCGGCTTCTG GTTCTGAACA GCTTAGCCTC GCACCAGGAC AGTTAATACT AATTCTAAAG 
AAAAACACAA GTGGGTGGTG GCAAGGGGAA TTACAGGCCA GAGGGAAAAA GCGACAGAAA 
GGATGGTTTC CTGCCAGCCA TGTTAAACTT TTGGGTCCAA GTAGTGAAAG AACTACACCT 
GCCTTTCATC CTGTATGTCA GGTGATTGCC ATGTATGACT ACACAGCAAA CAACGAGGAT 
GAGCTCAGTT TCTCCAAGGG ACAGCTTATT AATGTTTTAA ACAAGGATGA TCCTGACTGG 
TGGCAAGGAG AAATCAGTGG GGTGACTGGT CTCTTCCCTT CGAACTATGT TAAGATGACG 
ACGGATGCAG ATCCAAGTCA GCAGTGGTGT GCTGATCTCC AAACACTGGA CACCATGCAG 
CCGGTGGAAA GGAAGAGGCA GGGCTACATC CATGAGCTGA TTGAGACGGA GGAGCGCTAT 
GTGGACGACC TGCAGCTCGT CGTGGAGGTT TTTCAGAAAC GAATAACAGA GCCAGGTTTT 
CTCACTGAAG GAGAGATGGC CTTGATCTTT GTTAACTGGA AGGAGCTCAT CATGGCTAAC 
ACGAAACTGC TGAAGGCACT GCGGGTGCGG AAGAAGACCG GGGGTGAGAA GATGCCCGTG 
CACATGATCG GGGACATCCT GGCCGCTGAG CTGTCCCACA TGCAGGCCTA CATCCGCTTC 
TGCAGCTGTC AACTTAATGG AGCAGCCCTG TTACAGCAGA AGACAGACGA ACACGCAGAC 
TTCAAAGAAT TTTTAAAGAA GTTGGCATCT GACCCTCGGT GTAAAGGGAT GCCCCTTTCC 
AGCTTCCTGC TGAAGCCCAT GCAGAGGATC ACCCGCTACC CACTCCTCAT CAGAAGCATT 
CTGGAGAACA CCCCAGAGAA TCACGTAGAC CACTCCTCTC TGAAGCAGGC CCTCGAGCGG 
GCAGAGGAGC TCTGTTCTCA AGTGAATGAA GGCGTTCGGG AGAAGGAAAA TTCAGACCGA 
CTGGAGTGGA TCCAGACGCA CGTGCAGTGC GATGGGCTCG CGGAGCAACT GGTTTTCAAC 
TCCCTTACCA ACTGCCTGGG GCCCCGGAAG CTCCTGCACA GCGGAAAGCT GTACAAGACC 
AAGAGCAACA AGGAGCTGCA CGGGTTCCTC TTCAATGACT TCCTGCTTCT CACCTACATG 
GTCAAGCAGT TTGCGGTCTC CTCAGCCTCC GAGAAGCTTT TCAGCTCGAA GTCCAATGCT 
CAGTTCAAGA TGTATAAGAC GCCCATTTTC CTGAACGAAG CCTTGGTGAA ACTGCCCACA 
GACCCTTCCA GCGATGAGCC TGTCTTCCAC ATCTCTCACA TTGACCGGGT CTACACCCTC 
CGAACAGACA ACATTAACGA GAGGACAGCC TGGGTCCAGA AGATCAAGGC CGCGTCTGAG 
CAGTACATCG ACACGGAAAA GAAGAAGCGG GAGAAGGCCT ATCAAGCCCG CTCTCAGAAG 
TCTTCAGGCA TCGGGCGTCT GATGGTGCAT GTCATTGAAG CTACAGAACT GAAAGCCTGT 
AAACCAAATG GAAAGAGCAA TCCATACTGT GAAATCAGCA TGGGCTCCCA GAGCTACACC 
ACCAGAACCC TCCAGGACAC ACTAAATCCC AAATGGAATT TTAACTGCCA GTTCTTCATT 
AAGGACCTCT ATCAGGATGT GCTCTGTCTT ACCATGTTTG ACAGAGATCA GTTTTCACCA 
GACGATTTCC TGGGTCGCAC TGAAGTTCCA GTGGCAAAAA TTCGAACAGA ACAGGAAAGC 
AAAGGCCCTA CAACCCGCCG ACTCCTGCTG CACGAGGTCC CCACTGGGGA GGTCTGGGTC 
CGCTTTGACC TGCAGCTTTT TGAGCAGAAA ACTCTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN2 ( XM_012165654.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012021044.1 Ovis aries intersectin 2 (ITSN2), transcript variant X6, mRNA.

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CloneID	OSm29490
Clone ID Related Accession (Same CDS sequence)	XM_012165654.2
Accession Version	XM_012165654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	intersectin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012165654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGCATTGCCT 
CATACGTCAT CTTACAGTCT GATGATGGGA GGATTTGGTG GTGCTAATAT ACAGAAAGCC 
CAGTCTCTGA TTGATTTAGG ATCTAGTAGC TCAACTTCCT CAACTGCTTC CCTCTCAGGG 
AATTCACCCA AGACTGGGAC CTCAGAGTGG GCAGTTCCTC AGCCTTCAAG ATTAAAATAT 
CGGCAAAAAT TTAACAGTCT TGACAAAAGC ATGAGTGGAT ACCTCTCAGG TTTTCAAGCT 
AGAAATGCTC TTCTTCAGTC AAATCTTTCT CAAACTCAGC TAGCTACTAT ATGGACTCTG 
GCTGACATTG ATGGTGATGG ACAGCTGAAA GCTGAGGAAT TCATCCTTGC AATGCACCTC 
ACTGATATGG CCAAAGCTGG ACAGCCATTG CCACTGGCTT TACCTCCTGA GCTTGTCCCT 
CCATCTTTTA GAGGAGGAAA GCAGATTGAT TCCATTAACG GAACTCTGCC TTCATATCAG 
AAAACACAAG AAGAAGAACC TCAGAAAAAA TTACCAGTTA CGTTCGAGGA CAAGCGGAAA 
GCCAACTATG AGCGGGGCAA CATGGAACTG GAGAAGCGAC GCCAGGCCAT GATGGAGCAG 
CAGCAGAGGG AGGCAGAGCG CAAAGCCCAG AAAGAAAAGG AAGAATGGGA ACGAAAACAG 
AGAGAGCTAC AAGAGCAAGA ATGGAAGAAA CAACTTGAAT TAGAAAAACG CTTGGAGAAG 
CAGAGGGAAT TGGAGCGACA ACGGGAAGAA GAAAGGAGAA AAGAGATAGA AAGACGAGAG 
GCCGCAAAAC AGGAACTTGA AAGACAACGT CGTTTAGAAT GGGAGAGAAT TCGTAGGCAG 
GAGCTTCTCA GTCGAAGGAA TAGAGAACAG GAAGAAATTG TCAGGTTAAA CTCTAAAAAG 
AAGAGCCTCC ATCTCGAGCT GGAAGCACTG AATGGCAAAC ATCAACAGAT CTCAGGCAGA 
CTTCAAGACG TCCGACTCAG AAAGCAAACT CAGAAGACCG AGCTGGAAGT TCTGGATAAG 
CAGTGTGACC TGGAAATCAT GGAAATCAAG CAGCTTCAAC AGGAGCTTCA GGAGTATCAA 
AATAAACTTA TCTATTTGGT GCCAGAGAAG CAGTTATTAA ACGAAAGAAT TAAAAACATG 
CAGCTCAGTA ACACACCTGA TTTAGGGATC AGTTTACTTC ATAAAAAATC ATTAGAAAAG 
GAAGAATTAT GCCAAAGACT TAAAGAACAA TTAGATGCTC TTGAAAAAGA AACTGCATCC 
AAGCTTTCAG AAATGGATTC ATTTAACAAT GAACTGAAGT GTGGGAATGT GGATGACTCT 
GTTCTTCAGT GCCTTTTGTC TCTGCTAAGC TGTCTCAACA ACCTCTTCCT CTTACTTAAG 
GAACTGAGAG AAAGCTACAA TACACAGCAG TTAGCCATTG AACAGCTTTA TAAGATCAAA 
CGTGACAAAC TGAGGGAAAT TGAAAGAAAA AGATCAGAGT TAATACAGAA AAAGAAACTA 
GAAGATGAGG CTACAAGGAA AGCAAAACAA GGAAAAGAAA ACTTATGGAA AGAAAATCTT 
AGAAAGGAGG AAGAAGAAAA ACAAAAACGA CTCCAAGAAG AAAAAGCACA GGAAAAAGTT 
CAAGAAGTGG AGCGGAAAGC TGAGGAGAAA CAAAGTGAGC CGGCTGGTAC TTTGGTGAAT 
TACAGAGCAT TGTATCGCTT TGAAGCAAGG AACCATGATG AAATGAGTTT TAATTCTGGG 
GATATAATAC AGGTTGACGA AAAAACTGTA GGAGAACCTG GTTGGCTTTA TGGTGGTTTT 
CAAGGAAATT TTGGCTGGTT TCCATGCAAT TATGTAGAAA AAGTGACATC AAGTGAAAAA 
TCTGTGTCTC CTAAGAAGGC CTTACTTCCT CCTACAGTAT CTGCTACCTC AACTTCTGAA 
TCACTTTCTT CAAACCAACC AGCATCTGTG ACTGATTATC AAAATGTATC TTTTTCAAAC 
CTAAGTGTTA ACACATCATG GCAGAAAAAA TCAGCTTTTA CTCGCACTGT GTCCCCTGTG 
TCTGTATCAC CGATTCATGG ACAGGGACAG GTTGTGGAAA ACCTGAAAGC ACAGGCCCTT 
TGTTCTTGGA CAGCAAAGAA GGAAAACCAC TTGAACTTCT CAAAACATGA TATCATTACT 
GTTTTGGAGC AGCAAGAAAA TTGGTGGTTT GGGGAGGTTC ACGGAGGAAG AGGATGGTTT 
CCGAAATCTT ACGTCAAAAT CATCCCTGGG AGTGAAGTTA AGCGGGAAGA ACCAGAAGCT 
TTGTATGCAT CTGTAAACAA GAAACCTACC TCTGCAGCCT GTACAGTGGG AGAAGAGTAT 
ATTGCACTTT ATTCATATTC AAGTGTAGAA CCTGGAGATT TGACTTTCAC TGAAGGTGAA 
GAACTATTGG TGACCCAGAA AGATGGAGAA TGGTGGACAG GAAGTATTGG AGACAGAACT 
GGAATTTTTC CATCAAATTA TGTCAAACCA AAAGATCAGG AGGGTTTTGG GAGTGCTAGC 
AAATCTGGAA CATCAAACAA GAAACCTGAG ATTGCTCAAG TAACATCAGC ATATGCGGCT 
TCTGGTTCTG AACAGCTTAG CCTCGCACCA GGACAGTTAA TACTAATTCT AAAGAAAAAC 
ACAAGTGGGT GGTGGCAAGG GGAATTACAG GCCAGAGGGA AAAAGCGACA GAAAGGATGG 
TTTCCTGCCA GCCATGTTAA ACTTTTGGGT CCAAGTAGTG AAAGAACTAC ACCTGCCTTT 
CATCCTGTAT GTCAGGTGAT TGCCATGTAT GACTACACAG CAAACAACGA GGATGAGCTC 
AGTTTCTCCA AGGGACAGCT TATTAATGTT TTAAACAAGG ATGATCCTGA CTGGTGGCAA 
GGAGAAATCA GTGGGGTGAC TGGTCTCTTC CCTTCGAACT ATGTTAAGAT GACGACGGAT 
GCAGATCCAA GTCAGCAGTG GTGTGCTGAT CTCCAAACAC TGGACACCAT GCAGCCGGTG 
GAAAGGAAGA GGCAGGGCTA CATCCATGAG CTGATTGAGA CGGAGGAGCG CTATGTGGAC 
GACCTGCAGC TCGTCGTGGA GGTTTTTCAG AAACGAATAA CAGAGCCAGG TTTTCTCACT 
GAAGGAGAGA TGGCCTTGAT CTTTGTTAAC TGGAAGGAGC TCATCATGGC TAACACGAAA 
CTGCTGAAGG CACTGCGGGT GCGGAAGAAG ACCGGGGGTG AGAAGATGCC CGTGCACATG 
ATCGGGGACA TCCTGGCCGC TGAGCTGTCC CACATGCAGG CCTACATCCG CTTCTGCAGC 
TGTCAACTTA ATGGAGCAGC CCTGTTACAG CAGAAGACAG ACGAACACGC AGACTTCAAA 
GAATTTTTAA AGAAGTTGGC ATCTGACCCT CGGTGTAAAG GGATGCCCCT TTCCAGCTTC 
CTGCTGAAGC CCATGCAGAG GATCACCCGC TACCCACTCC TCATCAGAAG CATTCTGGAG 
AACACCCCAG AGAATCACGT AGACCACTCC TCTCTGAAGC AGGCCCTCGA GCGGGCAGAG 
GAGCTCTGTT CTCAAGTGAA TGAAGGCGTT CGGGAGAAGG AAAATTCAGA CCGACTGGAG 
TGGATCCAGA CGCACGTGCA GTGCGATGGG CTCGCGGAGC AACTGGTTTT CAACTCCCTT 
ACCAACTGCC TGGGGCCCCG GAAGCTCCTG CACAGCGGAA AGCTGTACAA GACCAAGAGC 
AACAAGGAGC TGCACGGGTT CCTCTTCAAT GACTTCCTGC TTCTCACCTA CATGGTCAAG 
CAGTTTGCGG TCTCCTCAGC CTCCGAGAAG CTTTTCAGCT CGAAGTCCAA TGCTCAGTTC 
AAGATGTATA AGACGCCCAT TTTCCTGAAC GAAGCCTTGG TGAAACTGCC CACAGACCCT 
TCCAGCGATG AGCCTGTCTT CCACATCTCT CACATTGACC GGGTCTACAC CCTCCGAACA 
GACAACATTA ACGAGAGGAC AGCCTGGGTC CAGAAGATCA AGGCCGCGTC TGAGCAGTAC 
ATCGACACGG AAAAGAAGAA GCGGGAGAAG GCCTATCAAG CCCGCTCTCA GAAGTCTTCA 
GGCATCGGGC GTCTGATGGT GCATGTCATT GAAGCTACAG AACTGAAAGC CTGTAAACCA 
AATGGAAAGA GCAATCCATA CTGTGAAATC AGCATGGGCT CCCAGAGCTA CACCACCAGA 
ACCCTCCAGG ACACACTAAA TCCCAAATGG AATTTTAACT GCCAGTTCTT CATTAAGGAC 
CTCTATCAGG ATGTGCTCTG TCTTACCATG TTTGACAGAG ATCAGTTTTC ACCAGACGAT 
TTCCTGGGTC GCACTGAAGT TCCAGTGGCA AAAATTCGAA CAGAACAGGA AAGCAAAGGC 
CCTACAACCC GCCGACTCCT GCTGCACGAG GTCCCCACTG GGGAGGTCTG GGTCCGCTTT 
GACCTGCAGC TTTTTGAGCA GAAAACTCTC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012021044.1
CDS	1852..6687
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFALPHTSSYSLMMGGFGGANIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLALPPELVPPSFRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQAMMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLSRRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDLGISLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNELKCGNVDDSVLQCLLSLLSCLNNLFLLLKELRESYNTQQLAIEQLYKIKRDKLREIERKRSELIQKKKLEDEATRKAKQGKENLWKENLRKEEEEKQKRLQEEKAQEKVQEVERKAEEKQSEPAGTLVNYRALYRFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGGFQGNFGWFPCNYVEKVTSSEKSVSPKKALLPPTVSATSTSESLSSNQPASVTDYQNVSFSNLSVNTSWQKKSAFTRTVSPVSVSPIHGQGQVVENLKAQALCSWTAKKENHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYASVNKKPTSAACTVGEEYIALYSYSSVEPGDLTFTEGEELLVTQKDGEWWTGSIGDRTGIFPSNYVKPKDQEGFGSASKSGTSNKKPEIAQVTSAYAASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTTPAFHPVCQVIAMYDYTANNEDELSFSKGQLINVLNKDDPDWWQGEISGVTGLFPSNYVKMTTDADPSQQWCADLQTLDTMQPVERKRQGYIHELIETEERYVDDLQLVVEVFQKRITEPGFLTEGEMALIFVNWKELIMANTKLLKALRVRKKTGGEKMPVHMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEHADFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPENHVDHSSLKQALERAEELCSQVNEGVREKENSDRLEWIQTHVQCDGLAEQLVFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSASEKLFSSKSNAQFKMYKTPIFLNEALVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKSSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_012165654.2
Organism	Ovis aries(sheep)
Definition	Ovis aries intersectin 2 (ITSN2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012165654.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCAATGAAT GGAGGGCCAA ACATGTGGGC TATTACCTCT 
GAAGAACGGA CTAAGCATGA CAAGCAGTTT GATAACCTCA AACCTTCGGG AGGTTATATA 
ACAGGTGATC AAGCCCGTAC CTTTTTCTTA CAGTCAGGCC TGCCGGCTCC CGTTTTAGCT 
GAAATATGGG CTTTATCAGA CCTGAACAAG GATGGGAAGA TGGATCAACA GGAGTTCTCC 
ATAGCGATGA AACTCATCAA ACTAAAGCTT CAAGGCCAAC AGTTGCCGGT GGTTCTTCCT 
CCTATTATGA AACAACCTCC TATGTTCTCT CCGTTAATTT CTGCTCGTTT TGCATTGCCT 
CATACGTCAT CTTACAGTCT GATGATGGGA GGATTTGGTG GTGCTAATAT ACAGAAAGCC 
CAGTCTCTGA TTGATTTAGG ATCTAGTAGC TCAACTTCCT CAACTGCTTC CCTCTCAGGG 
AATTCACCCA AGACTGGGAC CTCAGAGTGG GCAGTTCCTC AGCCTTCAAG ATTAAAATAT 
CGGCAAAAAT TTAACAGTCT TGACAAAAGC ATGAGTGGAT ACCTCTCAGG TTTTCAAGCT 
AGAAATGCTC TTCTTCAGTC AAATCTTTCT CAAACTCAGC TAGCTACTAT ATGGACTCTG 
GCTGACATTG ATGGTGATGG ACAGCTGAAA GCTGAGGAAT TCATCCTTGC AATGCACCTC 
ACTGATATGG CCAAAGCTGG ACAGCCATTG CCACTGGCTT TACCTCCTGA GCTTGTCCCT 
CCATCTTTTA GAGGAGGAAA GCAGATTGAT TCCATTAACG GAACTCTGCC TTCATATCAG 
AAAACACAAG AAGAAGAACC TCAGAAAAAA TTACCAGTTA CGTTCGAGGA CAAGCGGAAA 
GCCAACTATG AGCGGGGCAA CATGGAACTG GAGAAGCGAC GCCAGGCCAT GATGGAGCAG 
CAGCAGAGGG AGGCAGAGCG CAAAGCCCAG AAAGAAAAGG AAGAATGGGA ACGAAAACAG 
AGAGAGCTAC AAGAGCAAGA ATGGAAGAAA CAACTTGAAT TAGAAAAACG CTTGGAGAAG 
CAGAGGGAAT TGGAGCGACA ACGGGAAGAA GAAAGGAGAA AAGAGATAGA AAGACGAGAG 
GCCGCAAAAC AGGAACTTGA AAGACAACGT CGTTTAGAAT GGGAGAGAAT TCGTAGGCAG 
GAGCTTCTCA GTCGAAGGAA TAGAGAACAG GAAGAAATTG TCAGGTTAAA CTCTAAAAAG 
AAGAGCCTCC ATCTCGAGCT GGAAGCACTG AATGGCAAAC ATCAACAGAT CTCAGGCAGA 
CTTCAAGACG TCCGACTCAG AAAGCAAACT CAGAAGACCG AGCTGGAAGT TCTGGATAAG 
CAGTGTGACC TGGAAATCAT GGAAATCAAG CAGCTTCAAC AGGAGCTTCA GGAGTATCAA 
AATAAACTTA TCTATTTGGT GCCAGAGAAG CAGTTATTAA ACGAAAGAAT TAAAAACATG 
CAGCTCAGTA ACACACCTGA TTTAGGGATC AGTTTACTTC ATAAAAAATC ATTAGAAAAG 
GAAGAATTAT GCCAAAGACT TAAAGAACAA TTAGATGCTC TTGAAAAAGA AACTGCATCC 
AAGCTTTCAG AAATGGATTC ATTTAACAAT GAACTGAAGT GTGGGAATGT GGATGACTCT 
GTTCTTCAGT GCCTTTTGTC TCTGCTAAGC TGTCTCAACA ACCTCTTCCT CTTACTTAAG 
GAACTGAGAG AAAGCTACAA TACACAGCAG TTAGCCATTG AACAGCTTTA TAAGATCAAA 
CGTGACAAAC TGAGGGAAAT TGAAAGAAAA AGATCAGAGT TAATACAGAA AAAGAAACTA 
GAAGATGAGG CTACAAGGAA AGCAAAACAA GGAAAAGAAA ACTTATGGAA AGAAAATCTT 
AGAAAGGAGG AAGAAGAAAA ACAAAAACGA CTCCAAGAAG AAAAAGCACA GGAAAAAGTT 
CAAGAAGTGG AGCGGAAAGC TGAGGAGAAA CAAAGTGAGC CGGCTGGTAC TTTGGTGAAT 
TACAGAGCAT TGTATCGCTT TGAAGCAAGG AACCATGATG AAATGAGTTT TAATTCTGGG 
GATATAATAC AGGTTGACGA AAAAACTGTA GGAGAACCTG GTTGGCTTTA TGGTGGTTTT 
CAAGGAAATT TTGGCTGGTT TCCATGCAAT TATGTAGAAA AAGTGACATC AAGTGAAAAA 
TCTGTGTCTC CTAAGAAGGC CTTACTTCCT CCTACAGTAT CTGCTACCTC AACTTCTGAA 
TCACTTTCTT CAAACCAACC AGCATCTGTG ACTGATTATC AAAATGTATC TTTTTCAAAC 
CTAAGTGTTA ACACATCATG GCAGAAAAAA TCAGCTTTTA CTCGCACTGT GTCCCCTGTG 
TCTGTATCAC CGATTCATGG ACAGGGACAG GTTGTGGAAA ACCTGAAAGC ACAGGCCCTT 
TGTTCTTGGA CAGCAAAGAA GGAAAACCAC TTGAACTTCT CAAAACATGA TATCATTACT 
GTTTTGGAGC AGCAAGAAAA TTGGTGGTTT GGGGAGGTTC ACGGAGGAAG AGGATGGTTT 
CCGAAATCTT ACGTCAAAAT CATCCCTGGG AGTGAAGTTA AGCGGGAAGA ACCAGAAGCT 
TTGTATGCAT CTGTAAACAA GAAACCTACC TCTGCAGCCT GTACAGTGGG AGAAGAGTAT 
ATTGCACTTT ATTCATATTC AAGTGTAGAA CCTGGAGATT TGACTTTCAC TGAAGGTGAA 
GAACTATTGG TGACCCAGAA AGATGGAGAA TGGTGGACAG GAAGTATTGG AGACAGAACT 
GGAATTTTTC CATCAAATTA TGTCAAACCA AAAGATCAGG AGGGTTTTGG GAGTGCTAGC 
AAATCTGGAA CATCAAACAA GAAACCTGAG ATTGCTCAAG TAACATCAGC ATATGCGGCT 
TCTGGTTCTG AACAGCTTAG CCTCGCACCA GGACAGTTAA TACTAATTCT AAAGAAAAAC 
ACAAGTGGGT GGTGGCAAGG GGAATTACAG GCCAGAGGGA AAAAGCGACA GAAAGGATGG 
TTTCCTGCCA GCCATGTTAA ACTTTTGGGT CCAAGTAGTG AAAGAACTAC ACCTGCCTTT 
CATCCTGTAT GTCAGGTGAT TGCCATGTAT GACTACACAG CAAACAACGA GGATGAGCTC 
AGTTTCTCCA AGGGACAGCT TATTAATGTT TTAAACAAGG ATGATCCTGA CTGGTGGCAA 
GGAGAAATCA GTGGGGTGAC TGGTCTCTTC CCTTCGAACT ATGTTAAGAT GACGACGGAT 
GCAGATCCAA GTCAGCAGTG GTGTGCTGAT CTCCAAACAC TGGACACCAT GCAGCCGGTG 
GAAAGGAAGA GGCAGGGCTA CATCCATGAG CTGATTGAGA CGGAGGAGCG CTATGTGGAC 
GACCTGCAGC TCGTCGTGGA GGTTTTTCAG AAACGAATAA CAGAGCCAGG TTTTCTCACT 
GAAGGAGAGA TGGCCTTGAT CTTTGTTAAC TGGAAGGAGC TCATCATGGC TAACACGAAA 
CTGCTGAAGG CACTGCGGGT GCGGAAGAAG ACCGGGGGTG AGAAGATGCC CGTGCACATG 
ATCGGGGACA TCCTGGCCGC TGAGCTGTCC CACATGCAGG CCTACATCCG CTTCTGCAGC 
TGTCAACTTA ATGGAGCAGC CCTGTTACAG CAGAAGACAG ACGAACACGC AGACTTCAAA 
GAATTTTTAA AGAAGTTGGC ATCTGACCCT CGGTGTAAAG GGATGCCCCT TTCCAGCTTC 
CTGCTGAAGC CCATGCAGAG GATCACCCGC TACCCACTCC TCATCAGAAG CATTCTGGAG 
AACACCCCAG AGAATCACGT AGACCACTCC TCTCTGAAGC AGGCCCTCGA GCGGGCAGAG 
GAGCTCTGTT CTCAAGTGAA TGAAGGCGTT CGGGAGAAGG AAAATTCAGA CCGACTGGAG 
TGGATCCAGA CGCACGTGCA GTGCGATGGG CTCGCGGAGC AACTGGTTTT CAACTCCCTT 
ACCAACTGCC TGGGGCCCCG GAAGCTCCTG CACAGCGGAA AGCTGTACAA GACCAAGAGC 
AACAAGGAGC TGCACGGGTT CCTCTTCAAT GACTTCCTGC TTCTCACCTA CATGGTCAAG 
CAGTTTGCGG TCTCCTCAGC CTCCGAGAAG CTTTTCAGCT CGAAGTCCAA TGCTCAGTTC 
AAGATGTATA AGACGCCCAT TTTCCTGAAC GAAGCCTTGG TGAAACTGCC CACAGACCCT 
TCCAGCGATG AGCCTGTCTT CCACATCTCT CACATTGACC GGGTCTACAC CCTCCGAACA 
GACAACATTA ACGAGAGGAC AGCCTGGGTC CAGAAGATCA AGGCCGCGTC TGAGCAGTAC 
ATCGACACGG AAAAGAAGAA GCGGGAGAAG GCCTATCAAG CCCGCTCTCA GAAGTCTTCA 
GGCATCGGGC GTCTGATGGT GCATGTCATT GAAGCTACAG AACTGAAAGC CTGTAAACCA 
AATGGAAAGA GCAATCCATA CTGTGAAATC AGCATGGGCT CCCAGAGCTA CACCACCAGA 
ACCCTCCAGG ACACACTAAA TCCCAAATGG AATTTTAACT GCCAGTTCTT CATTAAGGAC 
CTCTATCAGG ATGTGCTCTG TCTTACCATG TTTGACAGAG ATCAGTTTTC ACCAGACGAT 
TTCCTGGGTC GCACTGAAGT TCCAGTGGCA AAAATTCGAA CAGAACAGGA AAGCAAAGGC 
CCTACAACCC GCCGACTCCT GCTGCACGAG GTCCCCACTG GGGAGGTCTG GGTCCGCTTT 
GACCTGCAGC TTTTTGAGCA GAAAACTCTC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ITSN2
Entrez Gene ID	101108644
Full Name	intersectin 2
Gene Type	protein-coding
Organism	Ovis aries(sheep)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ITSN2 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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