MIB2 gene cDNA ORF clone, Ovis aries(sheep)

mRNA	Protein	Name
XM_015099061.1	XP_014954547.1	LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase MIB2 isoform X1
XM_012138473.2	XP_011993863.1	E3 ubiquitin-protein ligase MIB2 isoform X2
XM_012138476.2	XP_011993866.1	E3 ubiquitin-protein ligase MIB2 isoform X2
XM_012138484.2	XP_011993874.1	E3 ubiquitin-protein ligase MIB2 isoform X10
XM_027975777.1	XP_027831578.1	E3 ubiquitin-protein ligase MIB2 isoform X2
XM_027975778.1	XP_027831579.1	E3 ubiquitin-protein ligase MIB2 isoform X3
XM_027975782.1	XP_027831583.1	E3 ubiquitin-protein ligase MIB2 isoform X6
XM_027975785.1	XP_027831586.1	E3 ubiquitin-protein ligase MIB2 isoform X8
XM_012138469.2	XP_011993859.1	E3 ubiquitin-protein ligase MIB2 isoform X2
XM_012138471.2	XP_011993861.1	E3 ubiquitin-protein ligase MIB2 isoform X2
XM_012138474.2	XP_011993864.1	E3 ubiquitin-protein ligase MIB2 isoform X2
XM_012138475.2	XP_011993865.1	E3 ubiquitin-protein ligase MIB2 isoform X2
XM_012138477.2	XP_011993867.1	E3 ubiquitin-protein ligase MIB2 isoform X3
XM_012138480.2	XP_011993870.1	E3 ubiquitin-protein ligase MIB2 isoform X6
XM_012138481.2	XP_011993871.1	E3 ubiquitin-protein ligase MIB2 isoform X7
XM_012138482.2	XP_011993872.1	E3 ubiquitin-protein ligase MIB2 isoform X8
XM_027975776.1	XP_027831577.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_012138479.2	XP_011993869.1	E3 ubiquitin-protein ligase MIB2 isoform X5
XM_027975783.1	XP_027831584.1	E3 ubiquitin-protein ligase MIB2 isoform X7
XM_027975787.1	XP_027831588.1	E3 ubiquitin-protein ligase MIB2 isoform X9
XM_012138483.2	XP_011993873.1	E3 ubiquitin-protein ligase MIB2 isoform X9
XM_012138485.2	XP_011993875.1	E3 ubiquitin-protein ligase MIB2 isoform X11
XM_027975779.1	XP_027831580.1	E3 ubiquitin-protein ligase MIB2 isoform X4
XM_027975781.1	XP_027831582.1	E3 ubiquitin-protein ligase MIB2 isoform X5
XM_027975786.1	XP_027831587.1	E3 ubiquitin-protein ligase MIB2 isoform X8
XM_012138478.2	XP_011993868.1	E3 ubiquitin-protein ligase MIB2 isoform X4
XM_012138486.2	XP_011993876.1	E3 ubiquitin-protein ligase MIB2 isoform X12
XM_027975780.1	XP_027831581.1	E3 ubiquitin-protein ligase MIB2 isoform X5
XM_027975784.1	XP_027831585.1	E3 ubiquitin-protein ligase MIB2 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv18831	XM_012138481.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OOv18832	XM_012138482.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OOv18833	XM_012138483.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OOv18836	XM_012138486.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OSm51769	XM_015099061.1 Latest version!	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OSm61767	XM_027975781.1 Latest version!	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSm61767	XM_027975780.1 Latest version!	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSm61768	XM_027975782.1 Latest version!	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSm61769	XM_027975784.1 Latest version!	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSm61769	XM_027975783.1 Latest version!	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSm61770	XM_027975786.1 Latest version!	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSm61770	XM_027975785.1 Latest version!	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSm61771	XM_027975787.1 Latest version!	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSm61763	XM_027975776.1 Latest version!	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSm61764	XM_027975777.1 Latest version!	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSm61765	XM_027975778.1 Latest version!	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSm61766	XM_027975779.1 Latest version!	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOv18826	XM_012138474.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OOv18826	XM_012138475.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OOv18826	XM_012138476.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OOv18826	XM_012138471.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OOv18826	XM_012138469.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OOv18826	XM_012138473.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OOv18827	XM_012138477.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OOv18828	XM_012138478.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OOv18829	XM_012138479.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OOv18830	XM_012138480.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OOv18834	XM_012138484.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OOv18835	XM_012138485.2 Latest version!	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

MIB2 ( XM_012138481.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993871.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OOv18831
Clone ID Related Accession (Same CDS sequence)	XM_012138481.2
Accession Version	XM_012138481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GACCCCTGTC CCCACGCAGG 
GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC 
AGTGCTCCGA ACACTGTGAC CAACCTGCAT GTGGCCACGC CGACGGGGCC TGAGGCCGCT 
GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG CTGGTGCTGT TCTCGCCCTG CCAGCATCGC 
ACAGTGTGCG AGGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC 
AGCAAGAAGC TGCGCCCAGA CGGCACGGAG GTGGTCAGCG CGGCCCCCGC GCCCGGCCCA 
CCACGGCAGT TGGTGGAGGA GCTGCAGAGC CGCTACCGGC AGATGGAGGA GCGCATCACC 
TGCCCGATTT GCATCGACAG CCACATCCGC CTCGTGTTCC AGTGCGGCCA TGGCGCGTGC 
GCGCCCTGTG GCGCGGCGCT CAGCGCCTGC CCCATCTGCC GCCAGCCCAT CCGCGATCGC 
ATCCAGATCT TCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993871.1
CDS	2179..5076
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRPLSPRRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGTEVVSAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_012138481.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138481.2

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GACCCCTGTC CCCACGCAGG 
GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC 
AGTGCTCCGA ACACTGTGAC CAACCTGCAT GTGGCCACGC CGACGGGGCC TGAGGCCGCT 
GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG CTGGTGCTGT TCTCGCCCTG CCAGCATCGC 
ACAGTGTGCG AGGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC 
AGCAAGAAGC TGCGCCCAGA CGGCACGGAG GTGGTCAGCG CGGCCCCCGC GCCCGGCCCA 
CCACGGCAGT TGGTGGAGGA GCTGCAGAGC CGCTACCGGC AGATGGAGGA GCGCATCACC 
TGCCCGATTT GCATCGACAG CCACATCCGC CTCGTGTTCC AGTGCGGCCA TGGCGCGTGC 
GCGCCCTGTG GCGCGGCGCT CAGCGCCTGC CCCATCTGCC GCCAGCCCAT CCGCGATCGC 
ATCCAGATCT TCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138482.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993872.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OOv18832
Clone ID Related Accession (Same CDS sequence)	XM_012138482.2
Accession Version	XM_012138482.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTTGCGGC 
GGTGGCAGCG GGGGCTCGGC CCAGGGCCCA AGACTGGTGC TCAGTGCTCC GAACACTGTG 
ACCAACCTGC ATGTGGCCAC GCCGACGGGG CCTGAGGCCG CTGAGTGCCT AGTGTGCTCA 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC 
GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA 
GACGGCACGG AGGTGGTCAG CGCGGCCCCC GCGCCCGGCC CACCACGGCA GTTGGTGGAG 
GAGCTGCAGA GCCGCTACCG GCAGATGGAG GAGCGCATCA CCTGCCCGAT TTGCATCGAC 
AGCCACATCC GCCTCGTGTT CCAGTGCGGC CATGGCGCGT GCGCGCCCTG TGGCGCGGCG 
CTCAGCGCCT GCCCCATCTG CCGCCAGCCC ATCCGCGATC GCATCCAGAT CTTCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993872.1
CDS	2178..5057
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGTEVVSAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_012138482.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138482.2

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTTGCGGC 
GGTGGCAGCG GGGGCTCGGC CCAGGGCCCA AGACTGGTGC TCAGTGCTCC GAACACTGTG 
ACCAACCTGC ATGTGGCCAC GCCGACGGGG CCTGAGGCCG CTGAGTGCCT AGTGTGCTCA 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC 
GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA 
GACGGCACGG AGGTGGTCAG CGCGGCCCCC GCGCCCGGCC CACCACGGCA GTTGGTGGAG 
GAGCTGCAGA GCCGCTACCG GCAGATGGAG GAGCGCATCA CCTGCCCGAT TTGCATCGAC 
AGCCACATCC GCCTCGTGTT CCAGTGCGGC CATGGCGCGT GCGCGCCCTG TGGCGCGGCG 
CTCAGCGCCT GCCCCATCTG CCGCCAGCCC ATCCGCGATC GCATCCAGAT CTTCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138483.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993873.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OOv18833
Clone ID Related Accession (Same CDS sequence)	XM_012138483.2
Accession Version	XM_012138483.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAGCCA CGAGACCCGC TGGACCTTCC ATCCTGGGGC TCTCACCAAG 
CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ACGACCTTGA CACGGTGAAG 
CGGCTGCAGG CTGGCCATGG GGAGTGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT 
GGGAGAGTGC TGAAGGTTTT TGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG 
TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC 
GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGTG TTGTCCTGGA CAAGCTTCGA 
GCCCAGAAGA GTGACCTGGA GCACCCAGGG AGGTTGGTGG TGGAGGTGGC TCTGGGCAAC 
ATGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG 
GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGTCAGG TGGAGCTGGT GCGGCTGCTG 
CTGCAGGCTC AGGCGGGCGT TGACCTGCCA GATGACGAGG GCAACACGGC ACTGCACTAC 
GCCGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCGGG TTGTGGGGCC 
AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CTGTGCAAAG GGGCTTCCTG 
GAGGTGGTGA AGGTCCTCTG TGAGCGTGGC TGTGATGTCA ACCTGCCTGA TGCCCATGCT 
GACACACCCC TGCACTGCGC CATCTCGGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC 
CTCACCGAGG TGCCAGGCAT CGACGTCACT GCCACCAACA GCCAGGGCTT CACCCTGCTG 
CACCATGCAT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC GCGGGCCCGG 
CAGCTGGTGG ACGCCAAGAA GGAGGATGGG TTCACGGCAC TGCACCTGGC CGCCTTCAAC 
AACCACGGGG AGGTGGCACA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC 
AACCGTAAGC TGCAGTCCCC GCTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG 
CCACTGCTGG TGGACGCCGG CTGCAGCGTC AACGCCGAGG ACGAGGAGGG GGACACGGCT 
CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG TAGCTGATGG GGGTGCAGGG 
GACCCGGGTC CCCTGCAGTT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC AGGCAGCGCG 
GAGCTGACGG CGGGCGCAGC GATAGCCTAC TTCTTAGCGC TGGAGGGCGC TGACCTGAGC 
TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA 
GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG 
GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTGAC CAACCTGCAT 
GTGGCCACGC CGACGGGGCC TGAGGCCGCT GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG 
CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGAGTGCGC GCGCAGGATG 
AAGAAATGCA TCAGGTGCCA GGTGGTCATC AGCAAGAAGC TGCGCCCAGA CGGCACGGAG 
GTGGTCAGCG CGGCCCCCGC GCCCGGCCCA CCACGGCAGT TGGTGGAGGA GCTGCAGAGC 
CGCTACCGGC AGATGGAGGA GCGCATCACC TGCCCGATTT GCATCGACAG CCACATCCGC 
CTCGTGTTCC AGTGCGGCCA TGGCGCGTGC GCGCCCTGTG GCGCGGCGCT CAGCGCCTGC 
CCCATCTGCC GCCAGCCCAT CCGCGATCGC ATCCAGATCT TCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993873.1
CDS	2179..5046
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGTEVVSAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_012138483.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138483.2

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAGCCA CGAGACCCGC TGGACCTTCC ATCCTGGGGC TCTCACCAAG 
CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ACGACCTTGA CACGGTGAAG 
CGGCTGCAGG CTGGCCATGG GGAGTGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT 
GGGAGAGTGC TGAAGGTTTT TGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG 
TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC 
GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGTG TTGTCCTGGA CAAGCTTCGA 
GCCCAGAAGA GTGACCTGGA GCACCCAGGG AGGTTGGTGG TGGAGGTGGC TCTGGGCAAC 
ATGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG 
GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGTCAGG TGGAGCTGGT GCGGCTGCTG 
CTGCAGGCTC AGGCGGGCGT TGACCTGCCA GATGACGAGG GCAACACGGC ACTGCACTAC 
GCCGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCGGG TTGTGGGGCC 
AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CTGTGCAAAG GGGCTTCCTG 
GAGGTGGTGA AGGTCCTCTG TGAGCGTGGC TGTGATGTCA ACCTGCCTGA TGCCCATGCT 
GACACACCCC TGCACTGCGC CATCTCGGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC 
CTCACCGAGG TGCCAGGCAT CGACGTCACT GCCACCAACA GCCAGGGCTT CACCCTGCTG 
CACCATGCAT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC GCGGGCCCGG 
CAGCTGGTGG ACGCCAAGAA GGAGGATGGG TTCACGGCAC TGCACCTGGC CGCCTTCAAC 
AACCACGGGG AGGTGGCACA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC 
AACCGTAAGC TGCAGTCCCC GCTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG 
CCACTGCTGG TGGACGCCGG CTGCAGCGTC AACGCCGAGG ACGAGGAGGG GGACACGGCT 
CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG TAGCTGATGG GGGTGCAGGG 
GACCCGGGTC CCCTGCAGTT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC AGGCAGCGCG 
GAGCTGACGG CGGGCGCAGC GATAGCCTAC TTCTTAGCGC TGGAGGGCGC TGACCTGAGC 
TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA 
GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG 
GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTGAC CAACCTGCAT 
GTGGCCACGC CGACGGGGCC TGAGGCCGCT GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG 
CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGAGTGCGC GCGCAGGATG 
AAGAAATGCA TCAGGTGCCA GGTGGTCATC AGCAAGAAGC TGCGCCCAGA CGGCACGGAG 
GTGGTCAGCG CGGCCCCCGC GCCCGGCCCA CCACGGCAGT TGGTGGAGGA GCTGCAGAGC 
CGCTACCGGC AGATGGAGGA GCGCATCACC TGCCCGATTT GCATCGACAG CCACATCCGC 
CTCGTGTTCC AGTGCGGCCA TGGCGCGTGC GCGCCCTGTG GCGCGGCGCT CAGCGCCTGC 
CCCATCTGCC GCCAGCCCAT CCGCGATCGC ATCCAGATCT TCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138486.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993876.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OOv18836
Clone ID Related Accession (Same CDS sequence)	XM_012138486.2
Accession Version	XM_012138486.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT 
ATCGACGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAGTG GACCGATGAC 
ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTTGGAGA CGGGAACGTG 
GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG 
AGTGTTGTCC TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAGGTTG 
GTGGTGGAGG TGGCTCTGGG CAACATGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA 
GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGT 
CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCAGATGAC 
GAGGGCAACA CGGCACTGCA CTACGCCGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG 
CTCTTGAGCT CGGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT 
GTGGCTGTGC AAAGGGGCTT CCTGGAGGTG GTGAAGGTCC TCTGTGAGCG TGGCTGTGAT 
GTCAACCTGC CTGATGCCCA TGCTGACACA CCCCTGCACT GCGCCATCTC GGCGGGCACT 
GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGACGT CACTGCCACC 
AACAGCCAGG GCTTCACCCT GCTGCACCAT GCATCCCTCA AAGGCCACAC ACTAGCTGTC 
AGGAGGATTC TGGCGCGGGC CCGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGGTTCACG 
GCACTGCACC TGGCCGCCTT CAACAACCAC GGGGAGGTGG CACAGGTTCT CATCCAAGAG 
GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCGCTGCA CCTGGCTGTG 
CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGACG CCGGCTGCAG CGTCAACGCC 
GAGGACGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT 
CTGGTAGCTG ATGGGGGTGC AGGGGACCCG GGTCCCCTGC AGTTGCTGTC CAGGCTACAG 
GCCTCGGGCC TTCCAGGCAG CGCGGAGCTG ACGGCGGGCG CAGCGATAGC CTACTTCTTA 
GCGCTGGAGG GCGCTGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG 
GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG 
CAGGCTTGCG GCGGTGGCAG CGGGGGCTCG GCCCAGGGCC CAAGACTGGT GCTCAGTGCT 
CCGAACACTG TGACCAACCT GCATGTGGCC ACGCCGACGG GGCCTGAGGC CGCTGAGTGC 
CTAGTGTGCT CAGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG 
TGCGAGGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG 
AAGCTGCGCC CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG 
CAGTTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG 
ATTTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC 
TGTGGCGCGG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG 
ATCTTCGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993876.1
CDS	2178..4949
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGTEVVSAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_012138486.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138486.2

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT 
ATCGACGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAGTG GACCGATGAC 
ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTTGGAGA CGGGAACGTG 
GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG 
AGTGTTGTCC TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAGGTTG 
GTGGTGGAGG TGGCTCTGGG CAACATGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA 
GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGT 
CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCAGATGAC 
GAGGGCAACA CGGCACTGCA CTACGCCGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG 
CTCTTGAGCT CGGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT 
GTGGCTGTGC AAAGGGGCTT CCTGGAGGTG GTGAAGGTCC TCTGTGAGCG TGGCTGTGAT 
GTCAACCTGC CTGATGCCCA TGCTGACACA CCCCTGCACT GCGCCATCTC GGCGGGCACT 
GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGACGT CACTGCCACC 
AACAGCCAGG GCTTCACCCT GCTGCACCAT GCATCCCTCA AAGGCCACAC ACTAGCTGTC 
AGGAGGATTC TGGCGCGGGC CCGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGGTTCACG 
GCACTGCACC TGGCCGCCTT CAACAACCAC GGGGAGGTGG CACAGGTTCT CATCCAAGAG 
GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCGCTGCA CCTGGCTGTG 
CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGACG CCGGCTGCAG CGTCAACGCC 
GAGGACGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT 
CTGGTAGCTG ATGGGGGTGC AGGGGACCCG GGTCCCCTGC AGTTGCTGTC CAGGCTACAG 
GCCTCGGGCC TTCCAGGCAG CGCGGAGCTG ACGGCGGGCG CAGCGATAGC CTACTTCTTA 
GCGCTGGAGG GCGCTGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG 
GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG 
CAGGCTTGCG GCGGTGGCAG CGGGGGCTCG GCCCAGGGCC CAAGACTGGT GCTCAGTGCT 
CCGAACACTG TGACCAACCT GCATGTGGCC ACGCCGACGG GGCCTGAGGC CGCTGAGTGC 
CTAGTGTGCT CAGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG 
TGCGAGGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG 
AAGCTGCGCC CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG 
CAGTTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG 
ATTTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC 
TGTGGCGCGG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG 
ATCTTCGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_015099061.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014954547.1 Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

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CloneID	OSm51769
Clone ID Related Accession (Same CDS sequence)	XM_015099061.1
Accession Version	XM_015099061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639021.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 7% of CDS bases frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTGCCG 
CCACGGCAGC CTTCGACCCC GCACCGCACA GTGGTCGTGC AGTGGGACCA CGGCACCCGC 
ACCAACTACC GCGCGGGCTA CCAGGGCGCA CACGACCTGC TGCTCTATGA CAACGCCCAA 
ATAGGTGTCC GCCACCCCAA CATCATCTGC GACTGCTGCA AGAAGCACGG GCTGCGGGGC 
ATGCGCTGGA AATGCCGTAT CTGCTTCGAC TACGATCTCT GCACGCAGTG CTACATGCAC 
AACAAGCACG ACCTGGCCCA CGCCTTCGAG CGCTACGAGA CGGCCCACTC GCGCCCGGTC 
ATGCTGAGTC CCCGCCAGGG CCTCCCAAGG ATCCCGTTAA GGGGCATCTT CCAGGGGGCG 
AAGGTGGTTC GGGGCCCCGA CTGGGAGTGG GGCTCACAGG ATGGAGGAGA AGGGAAGCCA 
GGCCGAGTAG TGGACATCCG TGGCTGGGAC GTGGAGACAG GCCGGAGTGT GGCCAGGGGG 
ACATGGGCTG ATGGCACCNA CAACGTGTAT CGTGTGGGCC ACAAGGGCAA GGTGGACCTC 
AAGTGTGTGG GCGAGGCAGC TGGCGGCTTC TACTACAGGG AGCACCTCCC AAGGCTTTGC 
AAGCCTGCAG AGCTGCAGCG CAGGGTCAGT GCTGATGGCC AGCCCTTCCA GCGTGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACAGACGTG CTGAGGGAGA TGCAGGAAGG CCACGGCGGG 
TGGAACCCCC GGATGGCCAA GTTTATCGGA CAGACGGGCA CCGTGCACCG CATCACAGAC 
CACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTC 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCT GCAGGGGACC CGGGTCCCCT GCAGTTGCTG 
TCCAGGCTCG GCTCCCCAGC CAGGCGCAGC CTGAAAGCCA TCCCGTCGAG TCTTTTGCTC 
TCAGGACACC CGGCCCCCAG CCCCTACCCA GAACTGTCCA GACTTCGGTC CTGCTTGCGC 
GCCGCAGGGA GCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014954547.1
CDS	1537..3714
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELPPRQPSTPHRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVARGTWADGTnullNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLCKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDHRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSAGDPGPLQLLSRLGSPARRSLKAIPSSLLLSGHPAPSPYPELSRLRSCLRAAGSA

Target ORF information:

RefSeq Version	XM_015099061.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015099061.1

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTGCCG 
CCACGGCAGC CTTCGACCCC GCACCGCACA GTGGTCGTGC AGTGGGACCA CGGCACCCGC 
ACCAACTACC GCGCGGGCTA CCAGGGCGCA CACGACCTGC TGCTCTATGA CAACGCCCAA 
ATAGGTGTCC GCCACCCCAA CATCATCTGC GACTGCTGCA AGAAGCACGG GCTGCGGGGC 
ATGCGCTGGA AATGCCGTAT CTGCTTCGAC TACGATCTCT GCACGCAGTG CTACATGCAC 
AACAAGCACG ACCTGGCCCA CGCCTTCGAG CGCTACGAGA CGGCCCACTC GCGCCCGGTC 
ATGCTGAGTC CCCGCCAGGG CCTCCCAAGG ATCCCGTTAA GGGGCATCTT CCAGGGGGCG 
AAGGTGGTTC GGGGCCCCGA CTGGGAGTGG GGCTCACAGG ATGGAGGAGA AGGGAAGCCA 
GGCCGAGTAG TGGACATCCG TGGCTGGGAC GTGGAGACAG GCCGGAGTGT GGCCAGGGGG 
ACATGGGCTG ATGGCACCNA CAACGTGTAT CGTGTGGGCC ACAAGGGCAA GGTGGACCTC 
AAGTGTGTGG GCGAGGCAGC TGGCGGCTTC TACTACAGGG AGCACCTCCC AAGGCTTTGC 
AAGCCTGCAG AGCTGCAGCG CAGGGTCAGT GCTGATGGCC AGCCCTTCCA GCGTGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACAGACGTG CTGAGGGAGA TGCAGGAAGG CCACGGCGGG 
TGGAACCCCC GGATGGCCAA GTTTATCGGA CAGACGGGCA CCGTGCACCG CATCACAGAC 
CACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTC 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCT GCAGGGGACC CGGGTCCCCT GCAGTTGCTG 
TCCAGGCTCG GCTCCCCAGC CAGGCGCAGC CTGAAAGCCA TCCCGTCGAG TCTTTTGCTC 
TCAGGACACC CGGCCCCCAG CCCCTACCCA GAACTGTCCA GACTTCGGTC CTGCTTGCGC 
GCCGCAGGGA GCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_027975781.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027831582.1 Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.

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CloneID	OSm61767
Clone ID Related Accession (Same CDS sequence)	XM_027975781.1 , XM_027975780.1
Accession Version	XM_027975781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase MIB2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTTGCGGC 
GGTGGCAGCG GGGGCTCGGC CCAGGGCCCA AGACTGGTGC TCAGTGCTCC GAACACTGTG 
ACCAACCTGC ATGTGGCCAC GCCGACGGGG CCTGAGGCCG CTGAGTGCCT AGTGTGCTCA 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC 
GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA 
GACGGCACGG AGGTGGTCAG CGCGGCCCCC GCGCCCGGCC CACCACGGCA GTTGGTGGAG 
GAGCTGCAGA GCCGCTACCG GCAGATGGAG GAGCGCATCA CCTGCCCGAT TTGCATCGAC 
AGCCACATCC GCCTCGTGTT CCAGTGCGGC CATGGCGCGT GCGCGCCCTG TGGCGCGGCG 
CTCAGCGCCT GCCCCATCTG CCGCCAGCCC ATCCGCGATC GCATCCAGAT CTTCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027831582.1
CDS	450..3329
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGTEVVSAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_027975781.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027975781.1

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTTGCGGC 
GGTGGCAGCG GGGGCTCGGC CCAGGGCCCA AGACTGGTGC TCAGTGCTCC GAACACTGTG 
ACCAACCTGC ATGTGGCCAC GCCGACGGGG CCTGAGGCCG CTGAGTGCCT AGTGTGCTCA 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC 
GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA 
GACGGCACGG AGGTGGTCAG CGCGGCCCCC GCGCCCGGCC CACCACGGCA GTTGGTGGAG 
GAGCTGCAGA GCCGCTACCG GCAGATGGAG GAGCGCATCA CCTGCCCGAT TTGCATCGAC 
AGCCACATCC GCCTCGTGTT CCAGTGCGGC CATGGCGCGT GCGCGCCCTG TGGCGCGGCG 
CTCAGCGCCT GCCCCATCTG CCGCCAGCCC ATCCGCGATC GCATCCAGAT CTTCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_027975780.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027831581.1 Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSm61767
Clone ID Related Accession (Same CDS sequence)	XM_027975781.1 , XM_027975780.1
Accession Version	XM_027975780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase MIB2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTTGCGGC 
GGTGGCAGCG GGGGCTCGGC CCAGGGCCCA AGACTGGTGC TCAGTGCTCC GAACACTGTG 
ACCAACCTGC ATGTGGCCAC GCCGACGGGG CCTGAGGCCG CTGAGTGCCT AGTGTGCTCA 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC 
GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA 
GACGGCACGG AGGTGGTCAG CGCGGCCCCC GCGCCCGGCC CACCACGGCA GTTGGTGGAG 
GAGCTGCAGA GCCGCTACCG GCAGATGGAG GAGCGCATCA CCTGCCCGAT TTGCATCGAC 
AGCCACATCC GCCTCGTGTT CCAGTGCGGC CATGGCGCGT GCGCGCCCTG TGGCGCGGCG 
CTCAGCGCCT GCCCCATCTG CCGCCAGCCC ATCCGCGATC GCATCCAGAT CTTCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027831581.1
CDS	374..3253
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGTEVVSAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_027975780.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027975780.1

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTTGCGGC 
GGTGGCAGCG GGGGCTCGGC CCAGGGCCCA AGACTGGTGC TCAGTGCTCC GAACACTGTG 
ACCAACCTGC ATGTGGCCAC GCCGACGGGG CCTGAGGCCG CTGAGTGCCT AGTGTGCTCA 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC 
GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA 
GACGGCACGG AGGTGGTCAG CGCGGCCCCC GCGCCCGGCC CACCACGGCA GTTGGTGGAG 
GAGCTGCAGA GCCGCTACCG GCAGATGGAG GAGCGCATCA CCTGCCCGAT TTGCATCGAC 
AGCCACATCC GCCTCGTGTT CCAGTGCGGC CATGGCGCGT GCGCGCCCTG TGGCGCGGCG 
CTCAGCGCCT GCCCCATCTG CCGCCAGCCC ATCCGCGATC GCATCCAGAT CTTCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_027975782.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027831583.1 Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OSm61768
Clone ID Related Accession (Same CDS sequence)	XM_027975782.1
Accession Version	XM_027975782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase MIB2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTCA TGCTGAGTCC CCGCCAGGGC CTCCCAAGGA TCCCGTTAAG GGGCATCTTC 
CAGGGGGCGA AGGTGGTTCG GGGCCCCGAC TGGGAGTGGG GCTCACAGGA TGGAGGAGAA 
GGGAAGCCAG GCCGAGTAGT GGACATCCGT GGCTGGGACG TGGAGACAGG CCGGAGTGTG 
GCCAGTGTGA CATGGGCTGA TGGCACCACC AACGTGTATC GTGTGGGCCA CAAGGGCAAG 
GTGGACCTCA AGTGTGTGGG CGAGGCAGCT GGCGGCTTCT ACTACAGGGA GCACCTCCCA 
AGGCTTGGCA AGCCTGCAGA GCTGCAGCGC AGGGTCAGTG CTGATGGCCA GCCCTTCCAG 
CGTGGGGACA AGGTCAAGTG TCTGCTGGAC ACAGACGTGC TGAGGGAGAT GCAGGAAGGC 
CACGGCGGGT GGAACCCCCG GATGGCCAAG TTTATCGGAC AGACGGGCAC CGTGCACCGC 
ATCACAGACC GTGGGGACGT GCGTGTGCAG TTCAGCCACG AGACCCGCTG GACCTTCCAT 
CCTGGGGCTC TCACCAAGCA AAACTCCTGC TTGGTGGGTG AGGTGGTGCG GGTTATCGAC 
GACCTTGACA CGGTGAAGCG GCTGCAGGCT GGCCATGGGG AGTGGACCGA TGACATGGCC 
CCTGCTCTGG GCCGCATTGG GAGAGTGCTG AAGGTTTTTG GAGACGGGAA CGTGGGTGTG 
CTGGTCAGTG GGAAGCTGTG GACCTTCAGC CCCTCCTGCC TGGTGGCCTA CCGGCCTGAG 
GAAGATGCCA ACCTGGACGT AGCTGGGCGA GCCCGGGAGA ACAAAAGCTC CCTGAGTGTT 
GTCCTGGACA AGCTTCGAGC CCAGAAGAGT GACCTGGAGC ACCCAGGGAG GTTGGTGGTG 
GAGGTGGCTC TGGGCAACAT GGCCCGGGCT CTGGACCTGC TGCGGCGGCA CCCAGAACAG 
GTGGACACCA AGAATCAGGG CAGGACTGCC CTGCAGATGG CTGCCTACCT AGGTCAGGTG 
GAGCTGGTGC GGCTGCTGCT GCAGGCTCAG GCGGGCGTTG ACCTGCCAGA TGACGAGGGC 
AACACGGCAC TGCACTACGC CGCCCTGGGG AACCAGCCTG AGGCTGCCCG GGTGCTCTTG 
AGCTCGGGTT GTGGGGCCAA TGCTCTGAAC AGCACTCGGA GCTCAGCACT GCATGTGGCT 
GTGCAAAGGG GCTTCCTGGA GGTGGTGAAG GTCCTCTGTG AGCGTGGCTG TGATGTCAAC 
CTGCCTGATG CCCATGCTGA CACACCCCTG CACTGCGCCA TCTCGGCGGG CACTGGTGCC 
AGCGGCATTG TGGAGGTCCT CACCGAGGTG CCAGGCATCG ACGTCACTGC CACCAACAGC 
CAGGGCTTCA CCCTGCTGCA CCATGCATCC CTCAAAGGCC ACACACTAGC TGTCAGGAGG 
ATTCTGGCGC GGGCCCGGCA GCTGGTGGAC GCCAAGAAGG AGGATGGGTT CACGGCACTG 
CACCTGGCCG CCTTCAACAA CCACGGGGAG GTGGCACAGG TTCTCATCCA AGAGGGCCAC 
TGTGATGTGA ACGCTCGCAA CCGTAAGCTG CAGTCCCCGC TGCACCTGGC TGTGCAGCAG 
GCCCACGTGG GGCTGGTGCC ACTGCTGGTG GACGCCGGCT GCAGCGTCAA CGCCGAGGAC 
GAGGAGGGGG ACACGGCTCT GCACGTGGCC CTGCAGCGCC ACCAGCTGCT GCCTCTGGTA 
GCTGATGGGG GTGCAGGGGA CCCGGGTCCC CTGCAGTTGC TGTCCAGGCT ACAGGCCTCG 
GGCCTTCCAG GCAGCGCGGA GCTGACGGCG GGCGCAGCGA TAGCCTACTT CTTAGCGCTG 
GAGGGCGCTG ACCTGAGCTA CGCCAACCAC CGCGGCCGGA GCCCCCTGGA CCTGGCTGCC 
GAGGGTCGCC TGCTCAAAGC CCTGCAGGGC TGTGCTCAGC GCTTCCGGGA GCGGCAGGCT 
TGCGGCGGTG GCAGCGGGGG CTCGGCCCAG GGCCCAAGAC TGGTGCTCAG TGCTCCGAAC 
ACTGTGACCA ACCTGCATGT GGCCACGCCG ACGGGGCCTG AGGCCGCTGA GTGCCTAGTG 
TGCTCAGAGC TGGCGCTGCT GGTGCTGTTC TCGCCCTGCC AGCATCGCAC AGTGTGCGAG 
GGTGAGTGCG GGGTGGGGAG GGGGCGCGGC AGCTGGGCTG GACGGCCCCT CACTCAACCG 
TGCCCTCCCC CCCGCAGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT 
CATCAGCAAG AAGCTGCGCC CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG 
CCCACCACGG CAGTTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT 
CACCTGCCCG ATTTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC 
GTGCGCGCCC TGTGGCGCGG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA 
TCGCATCCAG ATCTTCGTGT AGCCGGCGCG CCCCCCCCCC CCCGCCCCTT TCCGGGGCCA 
TGCCCCGCAG CCGCCCTCGC AAGCCCTTTC TCTGTGTTTT ATAAAAAGAT TGCTGGACTT 
TGTGCCTGGT TGCCTGTCTG GGGCGGGGCC GTGCAGGTCC CCCAACCGGG GACCGTTCTG 
ACTGCACCTC TTACACCGTT GGCCCAGCGC TGGGTTCTGC GCTTTGGCCA AAGGCCTTGC 
AGGGCCTCTT TCCGATCCGT ATTCTGCCCT TCTTCCCAGG ACTCACGGGT CCAGAGTGCT 
GCCCGCACCC TGGCCGGAAA GAACGGGGCC CGGGCTTGTG GTGTCGTCCG GGGGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027831583.1
CDS	611..3490
Translation	MRVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEGECGVGRGRGSWAGRPLTQPCPPPRRVRAQDEEMHQVPGGHQQEAAPRRHGGGQRGPRARPTTAVGGGAAEPLPADGGAHHLPDLHRQPHPPRVPVRPWRVRALWRGAQRLPHLPPAHPRSHPDLRVAGAPPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_027975782.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027975782.1

ORF Insert Sequence:

ATGCGGGTCA TGCTGAGTCC CCGCCAGGGC CTCCCAAGGA TCCCGTTAAG GGGCATCTTC 
CAGGGGGCGA AGGTGGTTCG GGGCCCCGAC TGGGAGTGGG GCTCACAGGA TGGAGGAGAA 
GGGAAGCCAG GCCGAGTAGT GGACATCCGT GGCTGGGACG TGGAGACAGG CCGGAGTGTG 
GCCAGTGTGA CATGGGCTGA TGGCACCACC AACGTGTATC GTGTGGGCCA CAAGGGCAAG 
GTGGACCTCA AGTGTGTGGG CGAGGCAGCT GGCGGCTTCT ACTACAGGGA GCACCTCCCA 
AGGCTTGGCA AGCCTGCAGA GCTGCAGCGC AGGGTCAGTG CTGATGGCCA GCCCTTCCAG 
CGTGGGGACA AGGTCAAGTG TCTGCTGGAC ACAGACGTGC TGAGGGAGAT GCAGGAAGGC 
CACGGCGGGT GGAACCCCCG GATGGCCAAG TTTATCGGAC AGACGGGCAC CGTGCACCGC 
ATCACAGACC GTGGGGACGT GCGTGTGCAG TTCAGCCACG AGACCCGCTG GACCTTCCAT 
CCTGGGGCTC TCACCAAGCA AAACTCCTGC TTGGTGGGTG AGGTGGTGCG GGTTATCGAC 
GACCTTGACA CGGTGAAGCG GCTGCAGGCT GGCCATGGGG AGTGGACCGA TGACATGGCC 
CCTGCTCTGG GCCGCATTGG GAGAGTGCTG AAGGTTTTTG GAGACGGGAA CGTGGGTGTG 
CTGGTCAGTG GGAAGCTGTG GACCTTCAGC CCCTCCTGCC TGGTGGCCTA CCGGCCTGAG 
GAAGATGCCA ACCTGGACGT AGCTGGGCGA GCCCGGGAGA ACAAAAGCTC CCTGAGTGTT 
GTCCTGGACA AGCTTCGAGC CCAGAAGAGT GACCTGGAGC ACCCAGGGAG GTTGGTGGTG 
GAGGTGGCTC TGGGCAACAT GGCCCGGGCT CTGGACCTGC TGCGGCGGCA CCCAGAACAG 
GTGGACACCA AGAATCAGGG CAGGACTGCC CTGCAGATGG CTGCCTACCT AGGTCAGGTG 
GAGCTGGTGC GGCTGCTGCT GCAGGCTCAG GCGGGCGTTG ACCTGCCAGA TGACGAGGGC 
AACACGGCAC TGCACTACGC CGCCCTGGGG AACCAGCCTG AGGCTGCCCG GGTGCTCTTG 
AGCTCGGGTT GTGGGGCCAA TGCTCTGAAC AGCACTCGGA GCTCAGCACT GCATGTGGCT 
GTGCAAAGGG GCTTCCTGGA GGTGGTGAAG GTCCTCTGTG AGCGTGGCTG TGATGTCAAC 
CTGCCTGATG CCCATGCTGA CACACCCCTG CACTGCGCCA TCTCGGCGGG CACTGGTGCC 
AGCGGCATTG TGGAGGTCCT CACCGAGGTG CCAGGCATCG ACGTCACTGC CACCAACAGC 
CAGGGCTTCA CCCTGCTGCA CCATGCATCC CTCAAAGGCC ACACACTAGC TGTCAGGAGG 
ATTCTGGCGC GGGCCCGGCA GCTGGTGGAC GCCAAGAAGG AGGATGGGTT CACGGCACTG 
CACCTGGCCG CCTTCAACAA CCACGGGGAG GTGGCACAGG TTCTCATCCA AGAGGGCCAC 
TGTGATGTGA ACGCTCGCAA CCGTAAGCTG CAGTCCCCGC TGCACCTGGC TGTGCAGCAG 
GCCCACGTGG GGCTGGTGCC ACTGCTGGTG GACGCCGGCT GCAGCGTCAA CGCCGAGGAC 
GAGGAGGGGG ACACGGCTCT GCACGTGGCC CTGCAGCGCC ACCAGCTGCT GCCTCTGGTA 
GCTGATGGGG GTGCAGGGGA CCCGGGTCCC CTGCAGTTGC TGTCCAGGCT ACAGGCCTCG 
GGCCTTCCAG GCAGCGCGGA GCTGACGGCG GGCGCAGCGA TAGCCTACTT CTTAGCGCTG 
GAGGGCGCTG ACCTGAGCTA CGCCAACCAC CGCGGCCGGA GCCCCCTGGA CCTGGCTGCC 
GAGGGTCGCC TGCTCAAAGC CCTGCAGGGC TGTGCTCAGC GCTTCCGGGA GCGGCAGGCT 
TGCGGCGGTG GCAGCGGGGG CTCGGCCCAG GGCCCAAGAC TGGTGCTCAG TGCTCCGAAC 
ACTGTGACCA ACCTGCATGT GGCCACGCCG ACGGGGCCTG AGGCCGCTGA GTGCCTAGTG 
TGCTCAGAGC TGGCGCTGCT GGTGCTGTTC TCGCCCTGCC AGCATCGCAC AGTGTGCGAG 
GGTGAGTGCG GGGTGGGGAG GGGGCGCGGC AGCTGGGCTG GACGGCCCCT CACTCAACCG 
TGCCCTCCCC CCCGCAGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT 
CATCAGCAAG AAGCTGCGCC CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG 
CCCACCACGG CAGTTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT 
CACCTGCCCG ATTTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC 
GTGCGCGCCC TGTGGCGCGG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA 
TCGCATCCAG ATCTTCGTGT AGCCGGCGCG CCCCCCCCCC CCCGCCCCTT TCCGGGGCCA 
TGCCCCGCAG CCGCCCTCGC AAGCCCTTTC TCTGTGTTTT ATAAAAAGAT TGCTGGACTT 
TGTGCCTGGT TGCCTGTCTG GGGCGGGGCC GTGCAGGTCC CCCAACCGGG GACCGTTCTG 
ACTGCACCTC TTACACCGTT GGCCCAGCGC TGGGTTCTGC GCTTTGGCCA AAGGCCTTGC 
AGGGCCTCTT TCCGATCCGT ATTCTGCCCT TCTTCCCAGG ACTCACGGGT CCAGAGTGCT 
GCCCGCACCC TGGCCGGAAA GAACGGGGCC CGGGCTTGTG GTGTCGTCCG GGGGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_027975783.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027831584.1 Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OSm61769
Clone ID Related Accession (Same CDS sequence)	XM_027975783.1 , XM_027975784.1
Accession Version	XM_027975783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase MIB2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAGCCA CGAGACCCGC TGGACCTTCC ATCCTGGGGC TCTCACCAAG 
CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ACGACCTTGA CACGGTGAAG 
CGGCTGCAGG CTGGCCATGG GGAGTGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT 
GGGAGAGTGC TGAAGGTTTT TGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG 
TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC 
GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGTG TTGTCCTGGA CAAGCTTCGA 
GCCCAGAAGA GTGACCTGGA GCACCCAGGG AGGTTGGTGG TGGAGGTGGC TCTGGGCAAC 
ATGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG 
GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGTCAGG TGGAGCTGGT GCGGCTGCTG 
CTGCAGGCTC AGGCGGGCGT TGACCTGCCA GATGACGAGG GCAACACGGC ACTGCACTAC 
GCCGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCGGG TTGTGGGGCC 
AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CTGTGCAAAG GGGCTTCCTG 
GAGGTGGTGA AGGTCCTCTG TGAGCGTGGC TGTGATGTCA ACCTGCCTGA TGCCCATGCT 
GACACACCCC TGCACTGCGC CATCTCGGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC 
CTCACCGAGG TGCCAGGCAT CGACGTCACT GCCACCAACA GCCAGGGCTT CACCCTGCTG 
CACCATGCAT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC GCGGGCCCGG 
CAGCTGGTGG ACGCCAAGAA GGAGGATGGG TTCACGGCAC TGCACCTGGC CGCCTTCAAC 
AACCACGGGG AGGTGGCACA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC 
AACCGTAAGC TGCAGTCCCC GCTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG 
CCACTGCTGG TGGACGCCGG CTGCAGCGTC AACGCCGAGG ACGAGGAGGG GGACACGGCT 
CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG TAGCTGATGG GGGTGCAGGG 
GACCCGGGTC CCCTGCAGTT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC AGGCAGCGCG 
GAGCTGACGG CGGGCGCAGC GATAGCCTAC TTCTTAGCGC TGGAGGGCGC TGACCTGAGC 
TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA 
GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG 
GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTGAC CAACCTGCAT 
GTGGCCACGC CGACGGGGCC TGAGGCCGCT GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG 
CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGAGTGCGC GCGCAGGATG 
AAGAAATGCA TCAGGTGCCA GGTGGTCATC AGCAAGAAGC TGCGCCCAGA CGGCACGGAG 
GTGGTCAGCG CGGCCCCCGC GCCCGGCCCA CCACGGCAGT TGGTGGAGGA GCTGCAGAGC 
CGCTACCGGC AGATGGAGGA GCGCATCACC TGCCCGATTT GCATCGACAG CCACATCCGC 
CTCGTGTTCC AGTGCGGCCA TGGCGCGTGC GCGCCCTGTG GCGCGGCGCT CAGCGCCTGC 
CCCATCTGCC GCCAGCCCAT CCGCGATCGC ATCCAGATCT TCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027831584.1
CDS	380..3247
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGTEVVSAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_027975783.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027975783.1

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAGCCA CGAGACCCGC TGGACCTTCC ATCCTGGGGC TCTCACCAAG 
CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ACGACCTTGA CACGGTGAAG 
CGGCTGCAGG CTGGCCATGG GGAGTGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT 
GGGAGAGTGC TGAAGGTTTT TGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG 
TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC 
GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGTG TTGTCCTGGA CAAGCTTCGA 
GCCCAGAAGA GTGACCTGGA GCACCCAGGG AGGTTGGTGG TGGAGGTGGC TCTGGGCAAC 
ATGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG 
GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGTCAGG TGGAGCTGGT GCGGCTGCTG 
CTGCAGGCTC AGGCGGGCGT TGACCTGCCA GATGACGAGG GCAACACGGC ACTGCACTAC 
GCCGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCGGG TTGTGGGGCC 
AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CTGTGCAAAG GGGCTTCCTG 
GAGGTGGTGA AGGTCCTCTG TGAGCGTGGC TGTGATGTCA ACCTGCCTGA TGCCCATGCT 
GACACACCCC TGCACTGCGC CATCTCGGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC 
CTCACCGAGG TGCCAGGCAT CGACGTCACT GCCACCAACA GCCAGGGCTT CACCCTGCTG 
CACCATGCAT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC GCGGGCCCGG 
CAGCTGGTGG ACGCCAAGAA GGAGGATGGG TTCACGGCAC TGCACCTGGC CGCCTTCAAC 
AACCACGGGG AGGTGGCACA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC 
AACCGTAAGC TGCAGTCCCC GCTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG 
CCACTGCTGG TGGACGCCGG CTGCAGCGTC AACGCCGAGG ACGAGGAGGG GGACACGGCT 
CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG TAGCTGATGG GGGTGCAGGG 
GACCCGGGTC CCCTGCAGTT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC AGGCAGCGCG 
GAGCTGACGG CGGGCGCAGC GATAGCCTAC TTCTTAGCGC TGGAGGGCGC TGACCTGAGC 
TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA 
GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG 
GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTGAC CAACCTGCAT 
GTGGCCACGC CGACGGGGCC TGAGGCCGCT GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG 
CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGAGTGCGC GCGCAGGATG 
AAGAAATGCA TCAGGTGCCA GGTGGTCATC AGCAAGAAGC TGCGCCCAGA CGGCACGGAG 
GTGGTCAGCG CGGCCCCCGC GCCCGGCCCA CCACGGCAGT TGGTGGAGGA GCTGCAGAGC 
CGCTACCGGC AGATGGAGGA GCGCATCACC TGCCCGATTT GCATCGACAG CCACATCCGC 
CTCGTGTTCC AGTGCGGCCA TGGCGCGTGC GCGCCCTGTG GCGCGGCGCT CAGCGCCTGC 
CCCATCTGCC GCCAGCCCAT CCGCGATCGC ATCCAGATCT TCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_027975784.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027831585.1 Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OSm61769
Clone ID Related Accession (Same CDS sequence)	XM_027975783.1 , XM_027975784.1
Accession Version	XM_027975784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase MIB2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAGCCA CGAGACCCGC TGGACCTTCC ATCCTGGGGC TCTCACCAAG 
CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ACGACCTTGA CACGGTGAAG 
CGGCTGCAGG CTGGCCATGG GGAGTGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT 
GGGAGAGTGC TGAAGGTTTT TGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG 
TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC 
GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGTG TTGTCCTGGA CAAGCTTCGA 
GCCCAGAAGA GTGACCTGGA GCACCCAGGG AGGTTGGTGG TGGAGGTGGC TCTGGGCAAC 
ATGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG 
GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGTCAGG TGGAGCTGGT GCGGCTGCTG 
CTGCAGGCTC AGGCGGGCGT TGACCTGCCA GATGACGAGG GCAACACGGC ACTGCACTAC 
GCCGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCGGG TTGTGGGGCC 
AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CTGTGCAAAG GGGCTTCCTG 
GAGGTGGTGA AGGTCCTCTG TGAGCGTGGC TGTGATGTCA ACCTGCCTGA TGCCCATGCT 
GACACACCCC TGCACTGCGC CATCTCGGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC 
CTCACCGAGG TGCCAGGCAT CGACGTCACT GCCACCAACA GCCAGGGCTT CACCCTGCTG 
CACCATGCAT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC GCGGGCCCGG 
CAGCTGGTGG ACGCCAAGAA GGAGGATGGG TTCACGGCAC TGCACCTGGC CGCCTTCAAC 
AACCACGGGG AGGTGGCACA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC 
AACCGTAAGC TGCAGTCCCC GCTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG 
CCACTGCTGG TGGACGCCGG CTGCAGCGTC AACGCCGAGG ACGAGGAGGG GGACACGGCT 
CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG TAGCTGATGG GGGTGCAGGG 
GACCCGGGTC CCCTGCAGTT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC AGGCAGCGCG 
GAGCTGACGG CGGGCGCAGC GATAGCCTAC TTCTTAGCGC TGGAGGGCGC TGACCTGAGC 
TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA 
GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG 
GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTGAC CAACCTGCAT 
GTGGCCACGC CGACGGGGCC TGAGGCCGCT GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG 
CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGAGTGCGC GCGCAGGATG 
AAGAAATGCA TCAGGTGCCA GGTGGTCATC AGCAAGAAGC TGCGCCCAGA CGGCACGGAG 
GTGGTCAGCG CGGCCCCCGC GCCCGGCCCA CCACGGCAGT TGGTGGAGGA GCTGCAGAGC 
CGCTACCGGC AGATGGAGGA GCGCATCACC TGCCCGATTT GCATCGACAG CCACATCCGC 
CTCGTGTTCC AGTGCGGCCA TGGCGCGTGC GCGCCCTGTG GCGCGGCGCT CAGCGCCTGC 
CCCATCTGCC GCCAGCCCAT CCGCGATCGC ATCCAGATCT TCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027831585.1
CDS	148..3015
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGTEVVSAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_027975784.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027975784.1

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAGCCA CGAGACCCGC TGGACCTTCC ATCCTGGGGC TCTCACCAAG 
CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ACGACCTTGA CACGGTGAAG 
CGGCTGCAGG CTGGCCATGG GGAGTGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT 
GGGAGAGTGC TGAAGGTTTT TGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG 
TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC 
GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGTG TTGTCCTGGA CAAGCTTCGA 
GCCCAGAAGA GTGACCTGGA GCACCCAGGG AGGTTGGTGG TGGAGGTGGC TCTGGGCAAC 
ATGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG 
GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGTCAGG TGGAGCTGGT GCGGCTGCTG 
CTGCAGGCTC AGGCGGGCGT TGACCTGCCA GATGACGAGG GCAACACGGC ACTGCACTAC 
GCCGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCGGG TTGTGGGGCC 
AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CTGTGCAAAG GGGCTTCCTG 
GAGGTGGTGA AGGTCCTCTG TGAGCGTGGC TGTGATGTCA ACCTGCCTGA TGCCCATGCT 
GACACACCCC TGCACTGCGC CATCTCGGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC 
CTCACCGAGG TGCCAGGCAT CGACGTCACT GCCACCAACA GCCAGGGCTT CACCCTGCTG 
CACCATGCAT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC GCGGGCCCGG 
CAGCTGGTGG ACGCCAAGAA GGAGGATGGG TTCACGGCAC TGCACCTGGC CGCCTTCAAC 
AACCACGGGG AGGTGGCACA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC 
AACCGTAAGC TGCAGTCCCC GCTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG 
CCACTGCTGG TGGACGCCGG CTGCAGCGTC AACGCCGAGG ACGAGGAGGG GGACACGGCT 
CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG TAGCTGATGG GGGTGCAGGG 
GACCCGGGTC CCCTGCAGTT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC AGGCAGCGCG 
GAGCTGACGG CGGGCGCAGC GATAGCCTAC TTCTTAGCGC TGGAGGGCGC TGACCTGAGC 
TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA 
GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG 
GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTGAC CAACCTGCAT 
GTGGCCACGC CGACGGGGCC TGAGGCCGCT GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG 
CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGAGTGCGC GCGCAGGATG 
AAGAAATGCA TCAGGTGCCA GGTGGTCATC AGCAAGAAGC TGCGCCCAGA CGGCACGGAG 
GTGGTCAGCG CGGCCCCCGC GCCCGGCCCA CCACGGCAGT TGGTGGAGGA GCTGCAGAGC 
CGCTACCGGC AGATGGAGGA GCGCATCACC TGCCCGATTT GCATCGACAG CCACATCCGC 
CTCGTGTTCC AGTGCGGCCA TGGCGCGTGC GCGCCCTGTG GCGCGGCGCT CAGCGCCTGC 
CCCATCTGCC GCCAGCCCAT CCGCGATCGC ATCCAGATCT TCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_027975785.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027831586.1 Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.

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CloneID	OSm61770
Clone ID Related Accession (Same CDS sequence)	XM_027975785.1 , XM_027975786.1
Accession Version	XM_027975785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase MIB2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT 
ATCGACGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAGTG GACCGATGAC 
ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTTGGAGA CGGGAACGTG 
GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG 
AGTGTTGTCC TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAGGTTG 
GTGGTGGAGG TGGCTCTGGG CAACATGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA 
GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGT 
CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCAGATGAC 
GAGGGCAACA CGGCACTGCA CTACGCCGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG 
CTCTTGAGCT CGGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT 
GTGGCTGTGC AAAGGGGCTT CCTGGAGGTG GTGAAGGTCC TCTGTGAGCG TGGCTGTGAT 
GTCAACCTGC CTGATGCCCA TGCTGACACA CCCCTGCACT GCGCCATCTC GGCGGGCACT 
GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGACGT CACTGCCACC 
AACAGCCAGG GCTTCACCCT GCTGCACCAT GCATCCCTCA AAGGCCACAC ACTAGCTGTC 
AGGAGGATTC TGGCGCGGGC CCGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGGTTCACG 
GCACTGCACC TGGCCGCCTT CAACAACCAC GGGGAGGTGG CACAGGTTCT CATCCAAGAG 
GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCGCTGCA CCTGGCTGTG 
CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGACG CCGGCTGCAG CGTCAACGCC 
GAGGACGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT 
CTGGTAGCTG ATGGGGGTGC AGGGGACCCG GGTCCCCTGC AGTTGCTGTC CAGGCTACAG 
GCCTCGGGCC TTCCAGGCAG CGCGGAGCTG ACGGCGGGCG CAGCGATAGC CTACTTCTTA 
GCGCTGGAGG GCGCTGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG 
GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG 
CAGGCTTGCG GCGGTGGCAG CGGGGGCTCG GCCCAGGGCC CAAGACTGGT GCTCAGTGCT 
CCGAACACTG TGACCAACCT GCATGTGGCC ACGCCGACGG GGCCTGAGGC CGCTGAGTGC 
CTAGTGTGCT CAGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG 
TGCGAGGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG 
AAGCTGCGCC CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG 
CAGTTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG 
ATTTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC 
TGTGGCGCGG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG 
ATCTTCGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027831586.1
CDS	375..3146
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGTEVVSAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_027975785.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027975785.1

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT 
ATCGACGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAGTG GACCGATGAC 
ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTTGGAGA CGGGAACGTG 
GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG 
AGTGTTGTCC TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAGGTTG 
GTGGTGGAGG TGGCTCTGGG CAACATGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA 
GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGT 
CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCAGATGAC 
GAGGGCAACA CGGCACTGCA CTACGCCGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG 
CTCTTGAGCT CGGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT 
GTGGCTGTGC AAAGGGGCTT CCTGGAGGTG GTGAAGGTCC TCTGTGAGCG TGGCTGTGAT 
GTCAACCTGC CTGATGCCCA TGCTGACACA CCCCTGCACT GCGCCATCTC GGCGGGCACT 
GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGACGT CACTGCCACC 
AACAGCCAGG GCTTCACCCT GCTGCACCAT GCATCCCTCA AAGGCCACAC ACTAGCTGTC 
AGGAGGATTC TGGCGCGGGC CCGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGGTTCACG 
GCACTGCACC TGGCCGCCTT CAACAACCAC GGGGAGGTGG CACAGGTTCT CATCCAAGAG 
GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCGCTGCA CCTGGCTGTG 
CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGACG CCGGCTGCAG CGTCAACGCC 
GAGGACGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT 
CTGGTAGCTG ATGGGGGTGC AGGGGACCCG GGTCCCCTGC AGTTGCTGTC CAGGCTACAG 
GCCTCGGGCC TTCCAGGCAG CGCGGAGCTG ACGGCGGGCG CAGCGATAGC CTACTTCTTA 
GCGCTGGAGG GCGCTGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG 
GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG 
CAGGCTTGCG GCGGTGGCAG CGGGGGCTCG GCCCAGGGCC CAAGACTGGT GCTCAGTGCT 
CCGAACACTG TGACCAACCT GCATGTGGCC ACGCCGACGG GGCCTGAGGC CGCTGAGTGC 
CTAGTGTGCT CAGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG 
TGCGAGGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG 
AAGCTGCGCC CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG 
CAGTTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG 
ATTTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC 
TGTGGCGCGG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG 
ATCTTCGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_027975786.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027831587.1 Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OSm61770
Clone ID Related Accession (Same CDS sequence)	XM_027975785.1 , XM_027975786.1
Accession Version	XM_027975786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase MIB2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT 
ATCGACGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAGTG GACCGATGAC 
ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTTGGAGA CGGGAACGTG 
GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG 
AGTGTTGTCC TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAGGTTG 
GTGGTGGAGG TGGCTCTGGG CAACATGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA 
GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGT 
CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCAGATGAC 
GAGGGCAACA CGGCACTGCA CTACGCCGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG 
CTCTTGAGCT CGGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT 
GTGGCTGTGC AAAGGGGCTT CCTGGAGGTG GTGAAGGTCC TCTGTGAGCG TGGCTGTGAT 
GTCAACCTGC CTGATGCCCA TGCTGACACA CCCCTGCACT GCGCCATCTC GGCGGGCACT 
GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGACGT CACTGCCACC 
AACAGCCAGG GCTTCACCCT GCTGCACCAT GCATCCCTCA AAGGCCACAC ACTAGCTGTC 
AGGAGGATTC TGGCGCGGGC CCGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGGTTCACG 
GCACTGCACC TGGCCGCCTT CAACAACCAC GGGGAGGTGG CACAGGTTCT CATCCAAGAG 
GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCGCTGCA CCTGGCTGTG 
CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGACG CCGGCTGCAG CGTCAACGCC 
GAGGACGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT 
CTGGTAGCTG ATGGGGGTGC AGGGGACCCG GGTCCCCTGC AGTTGCTGTC CAGGCTACAG 
GCCTCGGGCC TTCCAGGCAG CGCGGAGCTG ACGGCGGGCG CAGCGATAGC CTACTTCTTA 
GCGCTGGAGG GCGCTGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG 
GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG 
CAGGCTTGCG GCGGTGGCAG CGGGGGCTCG GCCCAGGGCC CAAGACTGGT GCTCAGTGCT 
CCGAACACTG TGACCAACCT GCATGTGGCC ACGCCGACGG GGCCTGAGGC CGCTGAGTGC 
CTAGTGTGCT CAGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG 
TGCGAGGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG 
AAGCTGCGCC CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG 
CAGTTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG 
ATTTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC 
TGTGGCGCGG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG 
ATCTTCGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027831587.1
CDS	383..3154
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGTEVVSAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_027975786.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027975786.1

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT 
ATCGACGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAGTG GACCGATGAC 
ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTTGGAGA CGGGAACGTG 
GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG 
AGTGTTGTCC TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAGGTTG 
GTGGTGGAGG TGGCTCTGGG CAACATGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA 
GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGT 
CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCAGATGAC 
GAGGGCAACA CGGCACTGCA CTACGCCGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG 
CTCTTGAGCT CGGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT 
GTGGCTGTGC AAAGGGGCTT CCTGGAGGTG GTGAAGGTCC TCTGTGAGCG TGGCTGTGAT 
GTCAACCTGC CTGATGCCCA TGCTGACACA CCCCTGCACT GCGCCATCTC GGCGGGCACT 
GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGACGT CACTGCCACC 
AACAGCCAGG GCTTCACCCT GCTGCACCAT GCATCCCTCA AAGGCCACAC ACTAGCTGTC 
AGGAGGATTC TGGCGCGGGC CCGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGGTTCACG 
GCACTGCACC TGGCCGCCTT CAACAACCAC GGGGAGGTGG CACAGGTTCT CATCCAAGAG 
GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCGCTGCA CCTGGCTGTG 
CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGACG CCGGCTGCAG CGTCAACGCC 
GAGGACGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT 
CTGGTAGCTG ATGGGGGTGC AGGGGACCCG GGTCCCCTGC AGTTGCTGTC CAGGCTACAG 
GCCTCGGGCC TTCCAGGCAG CGCGGAGCTG ACGGCGGGCG CAGCGATAGC CTACTTCTTA 
GCGCTGGAGG GCGCTGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG 
GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG 
CAGGCTTGCG GCGGTGGCAG CGGGGGCTCG GCCCAGGGCC CAAGACTGGT GCTCAGTGCT 
CCGAACACTG TGACCAACCT GCATGTGGCC ACGCCGACGG GGCCTGAGGC CGCTGAGTGC 
CTAGTGTGCT CAGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG 
TGCGAGGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG 
AAGCTGCGCC CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG 
CAGTTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG 
ATTTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC 
TGTGGCGCGG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG 
ATCTTCGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_027975787.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027831588.1 Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OSm61771
Clone ID Related Accession (Same CDS sequence)	XM_027975787.1
Accession Version	XM_027975787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase MIB2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTCA TGCTGAGTCC CCGCCAGGGC CTCCCAAGGA TCCCGTTAAG GGGCATCTTC 
CAGGGGGCGA AGGTGGTTCG GGGCCCCGAC TGGGAGTGGG GCTCACAGGA TGGAGGAGAA 
GGGAAGCCAG GCCGAGTAGT GGACATCCGT GGCTGGGACG TGGAGACAGG CCGGAGTGTG 
GCCAGTGTGA CATGGGCTGA TGGCACCACC AACGTGTATC GTGTGGGCCA CAAGGGCAAG 
GTGGACCTCA AGTGTGTGGG CGAGGCAGCT GGCGGCTTCT ACTACAGGGA GCACCTCCCA 
AGGCTTGGCA AGCCTGCAGA GCTGCAGCGC AGGGTCAGTG CTGATGGCCA GCCCTTCCAG 
CGTGGGGACA AGGTCAAGTG TCTGCTGGAC ACAGACGTGC TGAGGGAGAT GCAGGAAGGC 
CACGGCGGGT GGAACCCCCG GATGGCCAAG TTTATCGGAC AGACGGGCAC CGTGCACCGC 
ATCACAGACC GTGGGGACGT GCGTGTGCAG TTCAGCCACG AGACCCGCTG GACCTTCCAT 
CCTGGGGCTC TCACCAAGCA AAACTCCTGC TTGGTGGGTG AGGTGGTGCG GGTTATCGAC 
GACCTTGACA CGGTGAAGCG GCTGCAGGCT GGCCATGGGG AGTGGACCGA TGACATGGCC 
CCTGCTCTGG GCCGCATTGG GAGAGTGCTG AAGGTTTTTG GAGACGGGAA CGTGGGTGTG 
CTGGTCAGTG GGAAGCTGTG GACCTTCAGC CCCTCCTGCC TGGTGGCCTA CCGGCCTGAG 
GAAGATGCCA ACCTGGACGT AGCTGGGCGA GCCCGGGAGA ACAAAAGCTC CCTGAGTGTT 
GTCCTGGACA AGCTTCGAGC CCAGAAGAGT GACCTGGAGC ACCCAGGGAG GTTGGTGGTG 
GAGGTGGCTC TGGGCAACAT GGCCCGGGCT CTGGACCTGC TGCGGCGGCA CCCAGAACAG 
GTGGACACCA AGAATCAGGG CAGGACTGCC CTGCAGATGG CTGCCTACCT AGGTCAGGTG 
GAGCTGGTGC GGCTGCTGCT GCAGGCTCAG GCGGGCGTTG ACCTGCCAGA TGACGAGGGC 
AACACGGCAC TGCACTACGC CGCCCTGGGG AACCAGCCTG AGGCTGCCCG GGTGCTCTTG 
AGCTCGGGTT GTGGGGCCAA TGCTCTGAAC AGCACTCGGA GCTCAGCACT GCATGTGGCT 
GTGCAAAGGG GCTTCCTGGA GGTGGTGAAG GTCCTCTGTG AGCGTGGCTG TGATGTCAAC 
CTGCCTGATG CCCATGCTGA CACACCCCTG CACTGCGCCA TCTCGGCGGG CACTGGTGCC 
AGCGGCATTG TGGAGGTCCT CACCGAGGTG CCAGGCATCG ACGTCACTGC CACCAACAGC 
CAGGGCTTCA CCCTGCTGCA CCATGCATCC CTCAAAGGCC ACACACTAGC TGTCAGGAGG 
ATTCTGGCGC GGGCCCGGCA GCTGGTGGAC GCCAAGAAGG AGGATGGGTT CACGGCACTG 
CACCTGGCCG CCTTCAACAA CCACGGGGAG GTGGCACAGG TTCTCATCCA AGAGGGCCAC 
TGTGATGTGA ACGCTCGCAA CCGTAAGCTG CAGTCCCCGC TGCACCTGGC TGTGCAGCAG 
GCCCACGTGG GGCTGGTGCC ACTGCTGGTG GACGCCGGCT GCAGCGTCAA CGCCGAGGAC 
GAGGAGGGGG ACACGGCTCT GCACGTGGCC CTGCAGCGCC ACCAGCTGCT GCCTCTGGTA 
GCTGATGGGG GTGCAGGGGA CCCGGGTCCC CTGCAGTTGC TGTCCAGGCT ACAGGCCTCG 
GGCCTTCCAG GCAGCGCGGA GCTGACGGCG GGCGCAGCGA TAGCCTACTT CTTAGCGCTG 
GAGGGCGCTG ACCTGAGCTA CGCCAACCAC CGCGGCCGGA GCCCCCTGGA CCTGGCTGCC 
GAGGGTCGCC TGCTCAAAGC CCTGCAGGGC TGTGCTCAGC GCTTCCGGGA GCGGCAGGCT 
TGCGGCGGTG GCAGCGGGGG CTCGGCCCAG GGCCCAAGAC TGGTGCTCAG TGCTCCGAAC 
ACTGTGACCA ACCTGCATGT GGCCACGCCG ACGGGGCCTG AGGCCGCTGA GTGCCTAGTG 
TGCTCAGAGC TGGCGCTGCT GGTGCTGTTC TCGCCCTGCC AGCATCGCAC AGTGTGCGAG 
GAGTGCGCGC GCAGGATGAA GAAATGCATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTG 
CGCCCAGACG GCACGGAGGT GGTCAGCGCG GCCCCCGCGC CCGGCCCACC ACGGCAGTTG 
GTGGAGGAGC TGCAGAGCCG CTACCGGCAG ATGGAGGAGC GCATCACCTG CCCGATTTGC 
ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCATG GCGCGTGCGC GCCCTGTGGC 
GCGGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC GCGATCGCAT CCAGATCTTC 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027831588.1
CDS	675..3140
Translation	MRVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGTEVVSAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_027975787.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027975787.1

ORF Insert Sequence:

ATGCGGGTCA TGCTGAGTCC CCGCCAGGGC CTCCCAAGGA TCCCGTTAAG GGGCATCTTC 
CAGGGGGCGA AGGTGGTTCG GGGCCCCGAC TGGGAGTGGG GCTCACAGGA TGGAGGAGAA 
GGGAAGCCAG GCCGAGTAGT GGACATCCGT GGCTGGGACG TGGAGACAGG CCGGAGTGTG 
GCCAGTGTGA CATGGGCTGA TGGCACCACC AACGTGTATC GTGTGGGCCA CAAGGGCAAG 
GTGGACCTCA AGTGTGTGGG CGAGGCAGCT GGCGGCTTCT ACTACAGGGA GCACCTCCCA 
AGGCTTGGCA AGCCTGCAGA GCTGCAGCGC AGGGTCAGTG CTGATGGCCA GCCCTTCCAG 
CGTGGGGACA AGGTCAAGTG TCTGCTGGAC ACAGACGTGC TGAGGGAGAT GCAGGAAGGC 
CACGGCGGGT GGAACCCCCG GATGGCCAAG TTTATCGGAC AGACGGGCAC CGTGCACCGC 
ATCACAGACC GTGGGGACGT GCGTGTGCAG TTCAGCCACG AGACCCGCTG GACCTTCCAT 
CCTGGGGCTC TCACCAAGCA AAACTCCTGC TTGGTGGGTG AGGTGGTGCG GGTTATCGAC 
GACCTTGACA CGGTGAAGCG GCTGCAGGCT GGCCATGGGG AGTGGACCGA TGACATGGCC 
CCTGCTCTGG GCCGCATTGG GAGAGTGCTG AAGGTTTTTG GAGACGGGAA CGTGGGTGTG 
CTGGTCAGTG GGAAGCTGTG GACCTTCAGC CCCTCCTGCC TGGTGGCCTA CCGGCCTGAG 
GAAGATGCCA ACCTGGACGT AGCTGGGCGA GCCCGGGAGA ACAAAAGCTC CCTGAGTGTT 
GTCCTGGACA AGCTTCGAGC CCAGAAGAGT GACCTGGAGC ACCCAGGGAG GTTGGTGGTG 
GAGGTGGCTC TGGGCAACAT GGCCCGGGCT CTGGACCTGC TGCGGCGGCA CCCAGAACAG 
GTGGACACCA AGAATCAGGG CAGGACTGCC CTGCAGATGG CTGCCTACCT AGGTCAGGTG 
GAGCTGGTGC GGCTGCTGCT GCAGGCTCAG GCGGGCGTTG ACCTGCCAGA TGACGAGGGC 
AACACGGCAC TGCACTACGC CGCCCTGGGG AACCAGCCTG AGGCTGCCCG GGTGCTCTTG 
AGCTCGGGTT GTGGGGCCAA TGCTCTGAAC AGCACTCGGA GCTCAGCACT GCATGTGGCT 
GTGCAAAGGG GCTTCCTGGA GGTGGTGAAG GTCCTCTGTG AGCGTGGCTG TGATGTCAAC 
CTGCCTGATG CCCATGCTGA CACACCCCTG CACTGCGCCA TCTCGGCGGG CACTGGTGCC 
AGCGGCATTG TGGAGGTCCT CACCGAGGTG CCAGGCATCG ACGTCACTGC CACCAACAGC 
CAGGGCTTCA CCCTGCTGCA CCATGCATCC CTCAAAGGCC ACACACTAGC TGTCAGGAGG 
ATTCTGGCGC GGGCCCGGCA GCTGGTGGAC GCCAAGAAGG AGGATGGGTT CACGGCACTG 
CACCTGGCCG CCTTCAACAA CCACGGGGAG GTGGCACAGG TTCTCATCCA AGAGGGCCAC 
TGTGATGTGA ACGCTCGCAA CCGTAAGCTG CAGTCCCCGC TGCACCTGGC TGTGCAGCAG 
GCCCACGTGG GGCTGGTGCC ACTGCTGGTG GACGCCGGCT GCAGCGTCAA CGCCGAGGAC 
GAGGAGGGGG ACACGGCTCT GCACGTGGCC CTGCAGCGCC ACCAGCTGCT GCCTCTGGTA 
GCTGATGGGG GTGCAGGGGA CCCGGGTCCC CTGCAGTTGC TGTCCAGGCT ACAGGCCTCG 
GGCCTTCCAG GCAGCGCGGA GCTGACGGCG GGCGCAGCGA TAGCCTACTT CTTAGCGCTG 
GAGGGCGCTG ACCTGAGCTA CGCCAACCAC CGCGGCCGGA GCCCCCTGGA CCTGGCTGCC 
GAGGGTCGCC TGCTCAAAGC CCTGCAGGGC TGTGCTCAGC GCTTCCGGGA GCGGCAGGCT 
TGCGGCGGTG GCAGCGGGGG CTCGGCCCAG GGCCCAAGAC TGGTGCTCAG TGCTCCGAAC 
ACTGTGACCA ACCTGCATGT GGCCACGCCG ACGGGGCCTG AGGCCGCTGA GTGCCTAGTG 
TGCTCAGAGC TGGCGCTGCT GGTGCTGTTC TCGCCCTGCC AGCATCGCAC AGTGTGCGAG 
GAGTGCGCGC GCAGGATGAA GAAATGCATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTG 
CGCCCAGACG GCACGGAGGT GGTCAGCGCG GCCCCCGCGC CCGGCCCACC ACGGCAGTTG 
GTGGAGGAGC TGCAGAGCCG CTACCGGCAG ATGGAGGAGC GCATCACCTG CCCGATTTGC 
ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCATG GCGCGTGCGC GCCCTGTGGC 
GCGGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC GCGATCGCAT CCAGATCTTC 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_027975776.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027831577.1 Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSm61763
Clone ID Related Accession (Same CDS sequence)	XM_027975776.1
Accession Version	XM_027975776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTTGCGGC 
GGTGGCAGCG GGGGCTCGGC CCAGGGCCCA AGACTGGTGC TCAGTGCTCC GAACACTGTG 
ACCAACCTGC ATGTGGCCAC GCCGACGGGG CCTGAGGCCG CTGAGTGCCT AGTGTGCTCA 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGGTGAG 
TGCGGGGTGG GGAGGGGGCG CGGCAGCTGG GCTGGACGGC CCCTCACTCA ACCGTGCCCT 
CCCCCCCGCA GAGTGCGCGC GCAGGATGAA GAAATGCATC AGGTGCCAGG TGGTCATCAG 
CAAGAAGCTG CGCCCAGACG GCACGGAGGT GGTCAGCGCG GCCCCCGCGC CCGGCCCACC 
ACGGCAGTTG GTGGAGGAGC TGCAGAGCCG CTACCGGCAG ATGGAGGAGC GCATCACCTG 
CCCGATTTGC ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCATG GCGCGTGCGC 
GCCCTGTGGC GCGGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC GCGATCGCAT 
CCAGATCTTC GTGTAGCCGG CGCGCCCCCC CCCCCCCGCC CCTTTCCGGG GCCATGCCCC 
GCAGCCGCCC TCGCAAGCCC TTTCTCTGTG TTTTATAAAA AGATTGCTGG ACTTTGTGCC 
TGGTTGCCTG TCTGGGGCGG GGCCGTGCAG GTCCCCCAAC CGGGGACCGT TCTGACTGCA 
CCTCTTACAC CGTTGGCCCA GCGCTGGGTT CTGCGCTTTG GCCAAAGGCC TTGCAGGGCC 
TCTTTCCGAT CCGTATTCTG CCCTTCTTCC CAGGACTCAC GGGTCCAGAG TGCTGCCCGC 
ACCCTGGCCG GAAAGAACGG GGCCCGGGCT TGTGGTGTCG TCCGGGGGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027831577.1
CDS	130..3423
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEGECGVGRGRGSWAGRPLTQPCPPPRRVRAQDEEMHQVPGGHQQEAAPRRHGGGQRGPRARPTTAVGGGAAEPLPADGGAHHLPDLHRQPHPPRVPVRPWRVRALWRGAQRLPHLPPAHPRSHPDLRVAGAPPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_027975776.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027975776.1

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTTGCGGC 
GGTGGCAGCG GGGGCTCGGC CCAGGGCCCA AGACTGGTGC TCAGTGCTCC GAACACTGTG 
ACCAACCTGC ATGTGGCCAC GCCGACGGGG CCTGAGGCCG CTGAGTGCCT AGTGTGCTCA 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGGTGAG 
TGCGGGGTGG GGAGGGGGCG CGGCAGCTGG GCTGGACGGC CCCTCACTCA ACCGTGCCCT 
CCCCCCCGCA GAGTGCGCGC GCAGGATGAA GAAATGCATC AGGTGCCAGG TGGTCATCAG 
CAAGAAGCTG CGCCCAGACG GCACGGAGGT GGTCAGCGCG GCCCCCGCGC CCGGCCCACC 
ACGGCAGTTG GTGGAGGAGC TGCAGAGCCG CTACCGGCAG ATGGAGGAGC GCATCACCTG 
CCCGATTTGC ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCATG GCGCGTGCGC 
GCCCTGTGGC GCGGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC GCGATCGCAT 
CCAGATCTTC GTGTAGCCGG CGCGCCCCCC CCCCCCCGCC CCTTTCCGGG GCCATGCCCC 
GCAGCCGCCC TCGCAAGCCC TTTCTCTGTG TTTTATAAAA AGATTGCTGG ACTTTGTGCC 
TGGTTGCCTG TCTGGGGCGG GGCCGTGCAG GTCCCCCAAC CGGGGACCGT TCTGACTGCA 
CCTCTTACAC CGTTGGCCCA GCGCTGGGTT CTGCGCTTTG GCCAAAGGCC TTGCAGGGCC 
TCTTTCCGAT CCGTATTCTG CCCTTCTTCC CAGGACTCAC GGGTCCAGAG TGCTGCCCGC 
ACCCTGGCCG GAAAGAACGG GGCCCGGGCT TGTGGTGTCG TCCGGGGGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_027975777.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027831578.1 Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

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CloneID	OSm61764
Clone ID Related Accession (Same CDS sequence)	XM_027975777.1
Accession Version	XM_027975777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAGCCA CGAGACCCGC TGGACCTTCC ATCCTGGGGC TCTCACCAAG 
CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ACGACCTTGA CACGGTGAAG 
CGGCTGCAGG CTGGCCATGG GGAGTGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT 
GGGAGAGTGC TGAAGGTTTT TGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG 
TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC 
GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGTG TTGTCCTGGA CAAGCTTCGA 
GCCCAGAAGA GTGACCTGGA GCACCCAGGG AGGTTGGTGG TGGAGGTGGC TCTGGGCAAC 
ATGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG 
GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGTCAGG TGGAGCTGGT GCGGCTGCTG 
CTGCAGGCTC AGGCGGGCGT TGACCTGCCA GATGACGAGG GCAACACGGC ACTGCACTAC 
GCCGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCGGG TTGTGGGGCC 
AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CTGTGCAAAG GGGCTTCCTG 
GAGGTGGTGA AGGTCCTCTG TGAGCGTGGC TGTGATGTCA ACCTGCCTGA TGCCCATGCT 
GACACACCCC TGCACTGCGC CATCTCGGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC 
CTCACCGAGG TGCCAGGCAT CGACGTCACT GCCACCAACA GCCAGGGCTT CACCCTGCTG 
CACCATGCAT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC GCGGGCCCGG 
CAGCTGGTGG ACGCCAAGAA GGAGGATGGG TTCACGGCAC TGCACCTGGC CGCCTTCAAC 
AACCACGGGG AGGTGGCACA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC 
AACCGTAAGC TGCAGTCCCC GCTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG 
CCACTGCTGG TGGACGCCGG CTGCAGCGTC AACGCCGAGG ACGAGGAGGG GGACACGGCT 
CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG TAGCTGATGG GGGTGCAGGG 
GACCCGGGTC CCCTGCAGTT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC AGGCAGCGCG 
GAGCTGACGG CGGGCGCAGC GATAGCCTAC TTCTTAGCGC TGGAGGGCGC TGACCTGAGC 
TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA 
GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG 
GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTGAC CAACCTGCAT 
GTGGCCACGC CGACGGGGCC TGAGGCCGCT GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG 
CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGGTGAGTG CGGGGTGGGG 
AGGGGGCGCG GCAGCTGGGC TGGACGGCCC CTCACTCAAC CGTGCCCTCC CCCCCGCAGA 
GTGCGCGCGC AGGATGAAGA AATGCATCAG GTGCCAGGTG GTCATCAGCA AGAAGCTGCG 
CCCAGACGGC ACGGAGGTGG TCAGCGCGGC CCCCGCGCCC GGCCCACCAC GGCAGTTGGT 
GGAGGAGCTG CAGAGCCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CGATTTGCAT 
CGACAGCCAC ATCCGCCTCG TGTTCCAGTG CGGCCATGGC GCGTGCGCGC CCTGTGGCGC 
GGCGCTCAGC GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GATCGCATCC AGATCTTCGT 
GTAGCCGGCG CGCCCCCCCC CCCCCGCCCC TTTCCGGGGC CATGCCCCGC AGCCGCCCTC 
GCAAGCCCTT TCTCTGTGTT TTATAAAAAG ATTGCTGGAC TTTGTGCCTG GTTGCCTGTC 
TGGGGCGGGG CCGTGCAGGT CCCCCAACCG GGGACCGTTC TGACTGCACC TCTTACACCG 
TTGGCCCAGC GCTGGGTTCT GCGCTTTGGC CAAAGGCCTT GCAGGGCCTC TTTCCGATCC 
GTATTCTGCC CTTCTTCCCA GGACTCACGG GTCCAGAGTG CTGCCCGCAC CCTGGCCGGA 
AAGAACGGGG CCCGGGCTTG TGGTGTCGTC CGGGGGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027831578.1
CDS	1284..4565
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEGECGVGRGRGSWAGRPLTQPCPPPRRVRAQDEEMHQVPGGHQQEAAPRRHGGGQRGPRARPTTAVGGGAAEPLPADGGAHHLPDLHRQPHPPRVPVRPWRVRALWRGAQRLPHLPPAHPRSHPDLRVAGAPPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_027975777.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027975777.1

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAGCCA CGAGACCCGC TGGACCTTCC ATCCTGGGGC TCTCACCAAG 
CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ACGACCTTGA CACGGTGAAG 
CGGCTGCAGG CTGGCCATGG GGAGTGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT 
GGGAGAGTGC TGAAGGTTTT TGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG 
TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC 
GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGTG TTGTCCTGGA CAAGCTTCGA 
GCCCAGAAGA GTGACCTGGA GCACCCAGGG AGGTTGGTGG TGGAGGTGGC TCTGGGCAAC 
ATGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG 
GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGTCAGG TGGAGCTGGT GCGGCTGCTG 
CTGCAGGCTC AGGCGGGCGT TGACCTGCCA GATGACGAGG GCAACACGGC ACTGCACTAC 
GCCGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCGGG TTGTGGGGCC 
AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CTGTGCAAAG GGGCTTCCTG 
GAGGTGGTGA AGGTCCTCTG TGAGCGTGGC TGTGATGTCA ACCTGCCTGA TGCCCATGCT 
GACACACCCC TGCACTGCGC CATCTCGGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC 
CTCACCGAGG TGCCAGGCAT CGACGTCACT GCCACCAACA GCCAGGGCTT CACCCTGCTG 
CACCATGCAT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC GCGGGCCCGG 
CAGCTGGTGG ACGCCAAGAA GGAGGATGGG TTCACGGCAC TGCACCTGGC CGCCTTCAAC 
AACCACGGGG AGGTGGCACA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC 
AACCGTAAGC TGCAGTCCCC GCTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG 
CCACTGCTGG TGGACGCCGG CTGCAGCGTC AACGCCGAGG ACGAGGAGGG GGACACGGCT 
CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG TAGCTGATGG GGGTGCAGGG 
GACCCGGGTC CCCTGCAGTT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC AGGCAGCGCG 
GAGCTGACGG CGGGCGCAGC GATAGCCTAC TTCTTAGCGC TGGAGGGCGC TGACCTGAGC 
TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA 
GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG 
GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTGAC CAACCTGCAT 
GTGGCCACGC CGACGGGGCC TGAGGCCGCT GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG 
CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGGTGAGTG CGGGGTGGGG 
AGGGGGCGCG GCAGCTGGGC TGGACGGCCC CTCACTCAAC CGTGCCCTCC CCCCCGCAGA 
GTGCGCGCGC AGGATGAAGA AATGCATCAG GTGCCAGGTG GTCATCAGCA AGAAGCTGCG 
CCCAGACGGC ACGGAGGTGG TCAGCGCGGC CCCCGCGCCC GGCCCACCAC GGCAGTTGGT 
GGAGGAGCTG CAGAGCCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CGATTTGCAT 
CGACAGCCAC ATCCGCCTCG TGTTCCAGTG CGGCCATGGC GCGTGCGCGC CCTGTGGCGC 
GGCGCTCAGC GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GATCGCATCC AGATCTTCGT 
GTAGCCGGCG CGCCCCCCCC CCCCCGCCCC TTTCCGGGGC CATGCCCCGC AGCCGCCCTC 
GCAAGCCCTT TCTCTGTGTT TTATAAAAAG ATTGCTGGAC TTTGTGCCTG GTTGCCTGTC 
TGGGGCGGGG CCGTGCAGGT CCCCCAACCG GGGACCGTTC TGACTGCACC TCTTACACCG 
TTGGCCCAGC GCTGGGTTCT GCGCTTTGGC CAAAGGCCTT GCAGGGCCTC TTTCCGATCC 
GTATTCTGCC CTTCTTCCCA GGACTCACGG GTCCAGAGTG CTGCCCGCAC CCTGGCCGGA 
AAGAACGGGG CCCGGGCTTG TGGTGTCGTC CGGGGGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_027975778.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027831579.1 Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSm61765
Clone ID Related Accession (Same CDS sequence)	XM_027975778.1
Accession Version	XM_027975778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase MIB2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT 
ATCGACGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAGTG GACCGATGAC 
ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTTGGAGA CGGGAACGTG 
GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG 
AGTGTTGTCC TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAGGTTG 
GTGGTGGAGG TGGCTCTGGG CAACATGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA 
GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGT 
CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCAGATGAC 
GAGGGCAACA CGGCACTGCA CTACGCCGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG 
CTCTTGAGCT CGGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT 
GTGGCTGTGC AAAGGGGCTT CCTGGAGGTG GTGAAGGTCC TCTGTGAGCG TGGCTGTGAT 
GTCAACCTGC CTGATGCCCA TGCTGACACA CCCCTGCACT GCGCCATCTC GGCGGGCACT 
GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGACGT CACTGCCACC 
AACAGCCAGG GCTTCACCCT GCTGCACCAT GCATCCCTCA AAGGCCACAC ACTAGCTGTC 
AGGAGGATTC TGGCGCGGGC CCGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGGTTCACG 
GCACTGCACC TGGCCGCCTT CAACAACCAC GGGGAGGTGG CACAGGTTCT CATCCAAGAG 
GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCGCTGCA CCTGGCTGTG 
CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGACG CCGGCTGCAG CGTCAACGCC 
GAGGACGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT 
CTGGTAGCTG ATGGGGGTGC AGGGGACCCG GGTCCCCTGC AGTTGCTGTC CAGGCTACAG 
GCCTCGGGCC TTCCAGGCAG CGCGGAGCTG ACGGCGGGCG CAGCGATAGC CTACTTCTTA 
GCGCTGGAGG GCGCTGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG 
GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG 
CAGGCTTGCG GCGGTGGCAG CGGGGGCTCG GCCCAGGGCC CAAGACTGGT GCTCAGTGCT 
CCGAACACTG TGACCAACCT GCATGTGGCC ACGCCGACGG GGCCTGAGGC CGCTGAGTGC 
CTAGTGTGCT CAGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG 
TGCGAGGGTG AGTGCGGGGT GGGGAGGGGG CGCGGCAGCT GGGCTGGACG GCCCCTCACT 
CAACCGTGCC CTCCCCCCCG CAGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA 
GGTGGTCATC AGCAAGAAGC TGCGCCCAGA CGGCACGGAG GTGGTCAGCG CGGCCCCCGC 
GCCCGGCCCA CCACGGCAGT TGGTGGAGGA GCTGCAGAGC CGCTACCGGC AGATGGAGGA 
GCGCATCACC TGCCCGATTT GCATCGACAG CCACATCCGC CTCGTGTTCC AGTGCGGCCA 
TGGCGCGTGC GCGCCCTGTG GCGCGGCGCT CAGCGCCTGC CCCATCTGCC GCCAGCCCAT 
CCGCGATCGC ATCCAGATCT TCGTGTAGCC GGCGCGCCCC CCCCCCCCCG CCCCTTTCCG 
GGGCCATGCC CCGCAGCCGC CCTCGCAAGC CCTTTCTCTG TGTTTTATAA AAAGATTGCT 
GGACTTTGTG CCTGGTTGCC TGTCTGGGGC GGGGCCGTGC AGGTCCCCCA ACCGGGGACC 
GTTCTGACTG CACCTCTTAC ACCGTTGGCC CAGCGCTGGG TTCTGCGCTT TGGCCAAAGG 
CCTTGCAGGG CCTCTTTCCG ATCCGTATTC TGCCCTTCTT CCCAGGACTC ACGGGTCCAG 
AGTGCTGCCC GCACCCTGGC CGGAAAGAAC GGGGCCCGGG CTTGTGGTGT CGTCCGGGGG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027831579.1
CDS	1284..4469
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEGECGVGRGRGSWAGRPLTQPCPPPRRVRAQDEEMHQVPGGHQQEAAPRRHGGGQRGPRARPTTAVGGGAAEPLPADGGAHHLPDLHRQPHPPRVPVRPWRVRALWRGAQRLPHLPPAHPRSHPDLRVAGAPPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_027975778.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027975778.1

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT 
ATCGACGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAGTG GACCGATGAC 
ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTTGGAGA CGGGAACGTG 
GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG 
AGTGTTGTCC TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAGGTTG 
GTGGTGGAGG TGGCTCTGGG CAACATGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA 
GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGT 
CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCAGATGAC 
GAGGGCAACA CGGCACTGCA CTACGCCGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG 
CTCTTGAGCT CGGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT 
GTGGCTGTGC AAAGGGGCTT CCTGGAGGTG GTGAAGGTCC TCTGTGAGCG TGGCTGTGAT 
GTCAACCTGC CTGATGCCCA TGCTGACACA CCCCTGCACT GCGCCATCTC GGCGGGCACT 
GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGACGT CACTGCCACC 
AACAGCCAGG GCTTCACCCT GCTGCACCAT GCATCCCTCA AAGGCCACAC ACTAGCTGTC 
AGGAGGATTC TGGCGCGGGC CCGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGGTTCACG 
GCACTGCACC TGGCCGCCTT CAACAACCAC GGGGAGGTGG CACAGGTTCT CATCCAAGAG 
GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCGCTGCA CCTGGCTGTG 
CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGACG CCGGCTGCAG CGTCAACGCC 
GAGGACGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT 
CTGGTAGCTG ATGGGGGTGC AGGGGACCCG GGTCCCCTGC AGTTGCTGTC CAGGCTACAG 
GCCTCGGGCC TTCCAGGCAG CGCGGAGCTG ACGGCGGGCG CAGCGATAGC CTACTTCTTA 
GCGCTGGAGG GCGCTGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG 
GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG 
CAGGCTTGCG GCGGTGGCAG CGGGGGCTCG GCCCAGGGCC CAAGACTGGT GCTCAGTGCT 
CCGAACACTG TGACCAACCT GCATGTGGCC ACGCCGACGG GGCCTGAGGC CGCTGAGTGC 
CTAGTGTGCT CAGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG 
TGCGAGGGTG AGTGCGGGGT GGGGAGGGGG CGCGGCAGCT GGGCTGGACG GCCCCTCACT 
CAACCGTGCC CTCCCCCCCG CAGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA 
GGTGGTCATC AGCAAGAAGC TGCGCCCAGA CGGCACGGAG GTGGTCAGCG CGGCCCCCGC 
GCCCGGCCCA CCACGGCAGT TGGTGGAGGA GCTGCAGAGC CGCTACCGGC AGATGGAGGA 
GCGCATCACC TGCCCGATTT GCATCGACAG CCACATCCGC CTCGTGTTCC AGTGCGGCCA 
TGGCGCGTGC GCGCCCTGTG GCGCGGCGCT CAGCGCCTGC CCCATCTGCC GCCAGCCCAT 
CCGCGATCGC ATCCAGATCT TCGTGTAGCC GGCGCGCCCC CCCCCCCCCG CCCCTTTCCG 
GGGCCATGCC CCGCAGCCGC CCTCGCAAGC CCTTTCTCTG TGTTTTATAA AAAGATTGCT 
GGACTTTGTG CCTGGTTGCC TGTCTGGGGC GGGGCCGTGC AGGTCCCCCA ACCGGGGACC 
GTTCTGACTG CACCTCTTAC ACCGTTGGCC CAGCGCTGGG TTCTGCGCTT TGGCCAAAGG 
CCTTGCAGGG CCTCTTTCCG ATCCGTATTC TGCCCTTCTT CCCAGGACTC ACGGGTCCAG 
AGTGCTGCCC GCACCCTGGC CGGAAAGAAC GGGGCCCGGG CTTGTGGTGT CGTCCGGGGG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_027975779.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027831580.1 Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSm61766
Clone ID Related Accession (Same CDS sequence)	XM_027975779.1
Accession Version	XM_027975779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase MIB2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACGC CCAAATCGGT GTCCGCCACC CCAACATCAT CTGCGACTGC TGCAAGAAGC 
ACGGGCTGCG GGGCATGCGC TGGAAATGCC GTATCTGCTT CGACTACGAT CTCTGCACGC 
AGTGCTACAT GCACAACAAG CACGACCTGG CCCACGCCTT CGAGCGCTAC GAGACGGCCC 
ACTCGCGCCC GTCAAGTCTG TCCTGAGCCA TTTCCTGGAG GAGTGACCAG GGTGACGATC 
ACCCCAGGGG CCTGGGCATT GACACCAGCC CCCACCCCCT ACAGAAGGCG ACTGTGTCCA 
CCTAGCAGGG TCATGCTGAG TCCCCGCCAG GGCCTCCCAA GGATCCCGTT AAGGGGCATC 
TTCCAGGGGG CGAAGGTGGT TCGGGGCCCC GACTGGGAGT GGGGCTCACA GGATGGAGGA 
GAAGGGAAGC CAGGCCGAGT AGTGGACATC CGTGGCTGGG ACGTGGAGAC AGGCCGGAGT 
GTGGCCAGTG TGACATGGGC TGATGGCACC ACCAACGTGT ATCGTGTGGG CCACAAGGGC 
AAGGTGGACC TCAAGTGTGT GGGCGAGGCA GCTGGCGGCT TCTACTACAG GGAGCACCTC 
CCAAGGCTTG GCAAGCCTGC AGAGCTGCAG CGCAGGGTCA GTGCTGATGG CCAGCCCTTC 
CAGCGTGGGG ACAAGGTCAA GTGTCTGCTG GACACAGACG TGCTGAGGGA GATGCAGGAA 
GGCCACGGCG GGTGGAACCC CCGGATGGCC AAGTTTATCG GACAGACGGG CACCGTGCAC 
CGCATCACAG ACCGTGGGGA CGTGCGTGTG CAGTTCAGCC ACGAGACCCG CTGGACCTTC 
CATCCTGGGG CTCTCACCAA GCAAAACTCC TGCTTGGTGG GTGAGGTGGT GCGGGTTATC 
GACGACCTTG ACACGGTGAA GCGGCTGCAG GCTGGCCATG GGGAGTGGAC CGATGACATG 
GCCCCTGCTC TGGGCCGCAT TGGGAGAGTG CTGAAGGTTT TTGGAGACGG GAACGTGGGT 
GTGCTGGTCA GTGGGAAGCT GTGGACCTTC AGCCCCTCCT GCCTGGTGGC CTACCGGCCT 
GAGGAAGATG CCAACCTGGA CGTAGCTGGG CGAGCCCGGG AGAACAAAAG CTCCCTGAGT 
GTTGTCCTGG ACAAGCTTCG AGCCCAGAAG AGTGACCTGG AGCACCCAGG GAGGTTGGTG 
GTGGAGGTGG CTCTGGGCAA CATGGCCCGG GCTCTGGACC TGCTGCGGCG GCACCCAGAA 
CAGGTGGACA CCAAGAATCA GGGCAGGACT GCCCTGCAGA TGGCTGCCTA CCTAGGTCAG 
GTGGAGCTGG TGCGGCTGCT GCTGCAGGCT CAGGCGGGCG TTGACCTGCC AGATGACGAG 
GGCAACACGG CACTGCACTA CGCCGCCCTG GGGAACCAGC CTGAGGCTGC CCGGGTGCTC 
TTGAGCTCGG GTTGTGGGGC CAATGCTCTG AACAGCACTC GGAGCTCAGC ACTGCATGTG 
GCTGTGCAAA GGGGCTTCCT GGAGGTGGTG AAGGTCCTCT GTGAGCGTGG CTGTGATGTC 
AACCTGCCTG ATGCCCATGC TGACACACCC CTGCACTGCG CCATCTCGGC GGGCACTGGT 
GCCAGCGGCA TTGTGGAGGT CCTCACCGAG GTGCCAGGCA TCGACGTCAC TGCCACCAAC 
AGCCAGGGCT TCACCCTGCT GCACCATGCA TCCCTCAAAG GCCACACACT AGCTGTCAGG 
AGGATTCTGG CGCGGGCCCG GCAGCTGGTG GACGCCAAGA AGGAGGATGG GTTCACGGCA 
CTGCACCTGG CCGCCTTCAA CAACCACGGG GAGGTGGCAC AGGTTCTCAT CCAAGAGGGC 
CACTGTGATG TGAACGCTCG CAACCGTAAG CTGCAGTCCC CGCTGCACCT GGCTGTGCAG 
CAGGCCCACG TGGGGCTGGT GCCACTGCTG GTGGACGCCG GCTGCAGCGT CAACGCCGAG 
GACGAGGAGG GGGACACGGC TCTGCACGTG GCCCTGCAGC GCCACCAGCT GCTGCCTCTG 
GTAGCTGATG GGGGTGCAGG GGACCCGGGT CCCCTGCAGT TGCTGTCCAG GCTACAGGCC 
TCGGGCCTTC CAGGCAGCGC GGAGCTGACG GCGGGCGCAG CGATAGCCTA CTTCTTAGCG 
CTGGAGGGCG CTGACCTGAG CTACGCCAAC CACCGCGGCC GGAGCCCCCT GGACCTGGCT 
GCCGAGGGTC GCCTGCTCAA AGCCCTGCAG GGCTGTGCTC AGCGCTTCCG GGAGCGGCAG 
GCTTGCGGCG GTGGCAGCGG GGGCTCGGCC CAGGGCCCAA GACTGGTGCT CAGTGCTCCG 
AACACTGTGA CCAACCTGCA TGTGGCCACG CCGACGGGGC CTGAGGCCGC TGAGTGCCTA 
GTGTGCTCAG AGCTGGCGCT GCTGGTGCTG TTCTCGCCCT GCCAGCATCG CACAGTGTGC 
GAGGGTGAGT GCGGGGTGGG GAGGGGGCGC GGCAGCTGGG CTGGACGGCC CCTCACTCAA 
CCGTGCCCTC CCCCCCGCAG AGTGCGCGCG CAGGATGAAG AAATGCATCA GGTGCCAGGT 
GGTCATCAGC AAGAAGCTGC GCCCAGACGG CACGGAGGTG GTCAGCGCGG CCCCCGCGCC 
CGGCCCACCA CGGCAGTTGG TGGAGGAGCT GCAGAGCCGC TACCGGCAGA TGGAGGAGCG 
CATCACCTGC CCGATTTGCA TCGACAGCCA CATCCGCCTC GTGTTCCAGT GCGGCCATGG 
CGCGTGCGCG CCCTGTGGCG CGGCGCTCAG CGCCTGCCCC ATCTGCCGCC AGCCCATCCG 
CGATCGCATC CAGATCTTCG TGTAGCCGGC GCGCCCCCCC CCCCCCGCCC CTTTCCGGGG 
CCATGCCCCG CAGCCGCCCT CGCAAGCCCT TTCTCTGTGT TTTATAAAAA GATTGCTGGA 
CTTTGTGCCT GGTTGCCTGT CTGGGGCGGG GCCGTGCAGG TCCCCCAACC GGGGACCGTT 
CTGACTGCAC CTCTTACACC GTTGGCCCAG CGCTGGGTTC TGCGCTTTGG CCAAAGGCCT 
TGCAGGGCCT CTTTCCGATC CGTATTCTGC CCTTCTTCCC AGGACTCACG GGTCCAGAGT 
GCTGCCCGCA CCCTGGCCGG AAAGAACGGG GCCCGGGCTT GTGGTGTCGT CCGGGGGTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027831580.1
CDS	90..3272
Translation	MTTPKSVSATPTSSATAARSTGCGACAGNAVSASTTISARSATCTTSTTWPTPSSATRRPTRARQVCPEPFPGGVTRVTITPGAWALTPAPTPYRRRLCPPSRVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEGECGVGRGRGSWAGRPLTQPCPPPRRVRAQDEEMHQVPGGHQQEAAPRRHGGGQRGPRARPTTAVGGGAAEPLPADGGAHHLPDLHRQPHPPRVPVRPWRVRALWRGAQRLPHLPPAHPRSHPDLRVAGAPPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_027975779.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027975779.1

ORF Insert Sequence:

ATGACAACGC CCAAATCGGT GTCCGCCACC CCAACATCAT CTGCGACTGC TGCAAGAAGC 
ACGGGCTGCG GGGCATGCGC TGGAAATGCC GTATCTGCTT CGACTACGAT CTCTGCACGC 
AGTGCTACAT GCACAACAAG CACGACCTGG CCCACGCCTT CGAGCGCTAC GAGACGGCCC 
ACTCGCGCCC GTCAAGTCTG TCCTGAGCCA TTTCCTGGAG GAGTGACCAG GGTGACGATC 
ACCCCAGGGG CCTGGGCATT GACACCAGCC CCCACCCCCT ACAGAAGGCG ACTGTGTCCA 
CCTAGCAGGG TCATGCTGAG TCCCCGCCAG GGCCTCCCAA GGATCCCGTT AAGGGGCATC 
TTCCAGGGGG CGAAGGTGGT TCGGGGCCCC GACTGGGAGT GGGGCTCACA GGATGGAGGA 
GAAGGGAAGC CAGGCCGAGT AGTGGACATC CGTGGCTGGG ACGTGGAGAC AGGCCGGAGT 
GTGGCCAGTG TGACATGGGC TGATGGCACC ACCAACGTGT ATCGTGTGGG CCACAAGGGC 
AAGGTGGACC TCAAGTGTGT GGGCGAGGCA GCTGGCGGCT TCTACTACAG GGAGCACCTC 
CCAAGGCTTG GCAAGCCTGC AGAGCTGCAG CGCAGGGTCA GTGCTGATGG CCAGCCCTTC 
CAGCGTGGGG ACAAGGTCAA GTGTCTGCTG GACACAGACG TGCTGAGGGA GATGCAGGAA 
GGCCACGGCG GGTGGAACCC CCGGATGGCC AAGTTTATCG GACAGACGGG CACCGTGCAC 
CGCATCACAG ACCGTGGGGA CGTGCGTGTG CAGTTCAGCC ACGAGACCCG CTGGACCTTC 
CATCCTGGGG CTCTCACCAA GCAAAACTCC TGCTTGGTGG GTGAGGTGGT GCGGGTTATC 
GACGACCTTG ACACGGTGAA GCGGCTGCAG GCTGGCCATG GGGAGTGGAC CGATGACATG 
GCCCCTGCTC TGGGCCGCAT TGGGAGAGTG CTGAAGGTTT TTGGAGACGG GAACGTGGGT 
GTGCTGGTCA GTGGGAAGCT GTGGACCTTC AGCCCCTCCT GCCTGGTGGC CTACCGGCCT 
GAGGAAGATG CCAACCTGGA CGTAGCTGGG CGAGCCCGGG AGAACAAAAG CTCCCTGAGT 
GTTGTCCTGG ACAAGCTTCG AGCCCAGAAG AGTGACCTGG AGCACCCAGG GAGGTTGGTG 
GTGGAGGTGG CTCTGGGCAA CATGGCCCGG GCTCTGGACC TGCTGCGGCG GCACCCAGAA 
CAGGTGGACA CCAAGAATCA GGGCAGGACT GCCCTGCAGA TGGCTGCCTA CCTAGGTCAG 
GTGGAGCTGG TGCGGCTGCT GCTGCAGGCT CAGGCGGGCG TTGACCTGCC AGATGACGAG 
GGCAACACGG CACTGCACTA CGCCGCCCTG GGGAACCAGC CTGAGGCTGC CCGGGTGCTC 
TTGAGCTCGG GTTGTGGGGC CAATGCTCTG AACAGCACTC GGAGCTCAGC ACTGCATGTG 
GCTGTGCAAA GGGGCTTCCT GGAGGTGGTG AAGGTCCTCT GTGAGCGTGG CTGTGATGTC 
AACCTGCCTG ATGCCCATGC TGACACACCC CTGCACTGCG CCATCTCGGC GGGCACTGGT 
GCCAGCGGCA TTGTGGAGGT CCTCACCGAG GTGCCAGGCA TCGACGTCAC TGCCACCAAC 
AGCCAGGGCT TCACCCTGCT GCACCATGCA TCCCTCAAAG GCCACACACT AGCTGTCAGG 
AGGATTCTGG CGCGGGCCCG GCAGCTGGTG GACGCCAAGA AGGAGGATGG GTTCACGGCA 
CTGCACCTGG CCGCCTTCAA CAACCACGGG GAGGTGGCAC AGGTTCTCAT CCAAGAGGGC 
CACTGTGATG TGAACGCTCG CAACCGTAAG CTGCAGTCCC CGCTGCACCT GGCTGTGCAG 
CAGGCCCACG TGGGGCTGGT GCCACTGCTG GTGGACGCCG GCTGCAGCGT CAACGCCGAG 
GACGAGGAGG GGGACACGGC TCTGCACGTG GCCCTGCAGC GCCACCAGCT GCTGCCTCTG 
GTAGCTGATG GGGGTGCAGG GGACCCGGGT CCCCTGCAGT TGCTGTCCAG GCTACAGGCC 
TCGGGCCTTC CAGGCAGCGC GGAGCTGACG GCGGGCGCAG CGATAGCCTA CTTCTTAGCG 
CTGGAGGGCG CTGACCTGAG CTACGCCAAC CACCGCGGCC GGAGCCCCCT GGACCTGGCT 
GCCGAGGGTC GCCTGCTCAA AGCCCTGCAG GGCTGTGCTC AGCGCTTCCG GGAGCGGCAG 
GCTTGCGGCG GTGGCAGCGG GGGCTCGGCC CAGGGCCCAA GACTGGTGCT CAGTGCTCCG 
AACACTGTGA CCAACCTGCA TGTGGCCACG CCGACGGGGC CTGAGGCCGC TGAGTGCCTA 
GTGTGCTCAG AGCTGGCGCT GCTGGTGCTG TTCTCGCCCT GCCAGCATCG CACAGTGTGC 
GAGGGTGAGT GCGGGGTGGG GAGGGGGCGC GGCAGCTGGG CTGGACGGCC CCTCACTCAA 
CCGTGCCCTC CCCCCCGCAG AGTGCGCGCG CAGGATGAAG AAATGCATCA GGTGCCAGGT 
GGTCATCAGC AAGAAGCTGC GCCCAGACGG CACGGAGGTG GTCAGCGCGG CCCCCGCGCC 
CGGCCCACCA CGGCAGTTGG TGGAGGAGCT GCAGAGCCGC TACCGGCAGA TGGAGGAGCG 
CATCACCTGC CCGATTTGCA TCGACAGCCA CATCCGCCTC GTGTTCCAGT GCGGCCATGG 
CGCGTGCGCG CCCTGTGGCG CGGCGCTCAG CGCCTGCCCC ATCTGCCGCC AGCCCATCCG 
CGATCGCATC CAGATCTTCG TGTAGCCGGC GCGCCCCCCC CCCCCCGCCC CTTTCCGGGG 
CCATGCCCCG CAGCCGCCCT CGCAAGCCCT TTCTCTGTGT TTTATAAAAA GATTGCTGGA 
CTTTGTGCCT GGTTGCCTGT CTGGGGCGGG GCCGTGCAGG TCCCCCAACC GGGGACCGTT 
CTGACTGCAC CTCTTACACC GTTGGCCCAG CGCTGGGTTC TGCGCTTTGG CCAAAGGCCT 
TGCAGGGCCT CTTTCCGATC CGTATTCTGC CCTTCTTCCC AGGACTCACG GGTCCAGAGT 
GCTGCCCGCA CCCTGGCCGG AAAGAACGGG GCCCGGGCTT GTGGTGTCGT CCGGGGGTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138473.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993863.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOv18826
Clone ID Related Accession (Same CDS sequence)	XM_012138473.2 , XM_012138476.2 , XM_012138469.2 , XM_012138471.2 , XM_012138474.2 , XM_012138475.2
Accession Version	XM_012138473.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138473.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GACCCCTGTC CCCACGCAGG 
GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC 
AGTGCTCCGA ACACTGTGAC CAACCTGCAT GTGGCCACGC CGACGGGGCC TGAGGCCGCT 
GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG CTGGTGCTGT TCTCGCCCTG CCAGCATCGC 
ACAGTGTGCG AGGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC 
AGCAAGAAGC TGCGCCCAGG TGGGGCCCGG GGCCCGGCGC CCGCCCCGCC CCGCTGCCCC 
CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG CAGTTGGTGG 
AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG ATTTGCATCG 
ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC TGTGGCGCGG 
CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG ATCTTCGTGT 
AGCCGGCGCG CCCCCCCCCC CCGCCCCTTT CCGGGGCCAT GCCCCGCAGC CGCCCTCGCA 
AGCCCTTTCT CTGTGTTTTA TAAAAAGATT GCTGGACTTT GTGCCTGGTT GCCTGTCTGG 
GGCGGGGCCG TGCAGGTCCC CCAACCGGGG ACCGTTCTGA CTGCACCTCT TACACCGTTG 
GCCCAGCGCT GGGTTCTGCG CTTTGGCCAA AGGCCTTGCA GGGCTTCTTT CCGATCCGTA 
TTCTGCCCTT CTTCCCAGGA CTCACGGGTC CAGAGTGCTG CCCGCACCCT GGCCGGAAAG 
AACGGGGCCC GGGCTTGTGG TGTCGTCCGG GGGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993863.1
CDS	850..4128
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRPLSPRRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPGGARGPAPAPPRCPQTARRWSARPPRPAHHGSWWRSCRAATGRWRSASPARFASTATSASCSSAAMARARPVARRSAPAPSAASPSAIASRSSCSRRAPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_012138473.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138473.2

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GACCCCTGTC CCCACGCAGG 
GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC 
AGTGCTCCGA ACACTGTGAC CAACCTGCAT GTGGCCACGC CGACGGGGCC TGAGGCCGCT 
GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG CTGGTGCTGT TCTCGCCCTG CCAGCATCGC 
ACAGTGTGCG AGGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC 
AGCAAGAAGC TGCGCCCAGG TGGGGCCCGG GGCCCGGCGC CCGCCCCGCC CCGCTGCCCC 
CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG CAGTTGGTGG 
AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG ATTTGCATCG 
ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC TGTGGCGCGG 
CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG ATCTTCGTGT 
AGCCGGCGCG CCCCCCCCCC CCGCCCCTTT CCGGGGCCAT GCCCCGCAGC CGCCCTCGCA 
AGCCCTTTCT CTGTGTTTTA TAAAAAGATT GCTGGACTTT GTGCCTGGTT GCCTGTCTGG 
GGCGGGGCCG TGCAGGTCCC CCAACCGGGG ACCGTTCTGA CTGCACCTCT TACACCGTTG 
GCCCAGCGCT GGGTTCTGCG CTTTGGCCAA AGGCCTTGCA GGGCTTCTTT CCGATCCGTA 
TTCTGCCCTT CTTCCCAGGA CTCACGGGTC CAGAGTGCTG CCCGCACCCT GGCCGGAAAG 
AACGGGGCCC GGGCTTGTGG TGTCGTCCGG GGGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138476.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993866.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OOv18826
Clone ID Related Accession (Same CDS sequence)	XM_012138473.2 , XM_012138476.2 , XM_012138469.2 , XM_012138471.2 , XM_012138474.2 , XM_012138475.2
Accession Version	XM_012138476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GACCCCTGTC CCCACGCAGG 
GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC 
AGTGCTCCGA ACACTGTGAC CAACCTGCAT GTGGCCACGC CGACGGGGCC TGAGGCCGCT 
GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG CTGGTGCTGT TCTCGCCCTG CCAGCATCGC 
ACAGTGTGCG AGGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC 
AGCAAGAAGC TGCGCCCAGG TGGGGCCCGG GGCCCGGCGC CCGCCCCGCC CCGCTGCCCC 
CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG CAGTTGGTGG 
AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG ATTTGCATCG 
ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC TGTGGCGCGG 
CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG ATCTTCGTGT 
AGCCGGCGCG CCCCCCCCCC CCGCCCCTTT CCGGGGCCAT GCCCCGCAGC CGCCCTCGCA 
AGCCCTTTCT CTGTGTTTTA TAAAAAGATT GCTGGACTTT GTGCCTGGTT GCCTGTCTGG 
GGCGGGGCCG TGCAGGTCCC CCAACCGGGG ACCGTTCTGA CTGCACCTCT TACACCGTTG 
GCCCAGCGCT GGGTTCTGCG CTTTGGCCAA AGGCCTTGCA GGGCTTCTTT CCGATCCGTA 
TTCTGCCCTT CTTCCCAGGA CTCACGGGTC CAGAGTGCTG CCCGCACCCT GGCCGGAAAG 
AACGGGGCCC GGGCTTGTGG TGTCGTCCGG GGGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993866.1
CDS	707..3985
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRPLSPRRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPGGARGPAPAPPRCPQTARRWSARPPRPAHHGSWWRSCRAATGRWRSASPARFASTATSASCSSAAMARARPVARRSAPAPSAASPSAIASRSSCSRRAPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_012138476.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138476.2

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GACCCCTGTC CCCACGCAGG 
GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC 
AGTGCTCCGA ACACTGTGAC CAACCTGCAT GTGGCCACGC CGACGGGGCC TGAGGCCGCT 
GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG CTGGTGCTGT TCTCGCCCTG CCAGCATCGC 
ACAGTGTGCG AGGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC 
AGCAAGAAGC TGCGCCCAGG TGGGGCCCGG GGCCCGGCGC CCGCCCCGCC CCGCTGCCCC 
CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG CAGTTGGTGG 
AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG ATTTGCATCG 
ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC TGTGGCGCGG 
CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG ATCTTCGTGT 
AGCCGGCGCG CCCCCCCCCC CCGCCCCTTT CCGGGGCCAT GCCCCGCAGC CGCCCTCGCA 
AGCCCTTTCT CTGTGTTTTA TAAAAAGATT GCTGGACTTT GTGCCTGGTT GCCTGTCTGG 
GGCGGGGCCG TGCAGGTCCC CCAACCGGGG ACCGTTCTGA CTGCACCTCT TACACCGTTG 
GCCCAGCGCT GGGTTCTGCG CTTTGGCCAA AGGCCTTGCA GGGCTTCTTT CCGATCCGTA 
TTCTGCCCTT CTTCCCAGGA CTCACGGGTC CAGAGTGCTG CCCGCACCCT GGCCGGAAAG 
AACGGGGCCC GGGCTTGTGG TGTCGTCCGG GGGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138469.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993859.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

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CloneID	OOv18826
Clone ID Related Accession (Same CDS sequence)	XM_012138473.2 , XM_012138476.2 , XM_012138469.2 , XM_012138471.2 , XM_012138474.2 , XM_012138475.2
Accession Version	XM_012138469.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GACCCCTGTC CCCACGCAGG 
GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC 
AGTGCTCCGA ACACTGTGAC CAACCTGCAT GTGGCCACGC CGACGGGGCC TGAGGCCGCT 
GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG CTGGTGCTGT TCTCGCCCTG CCAGCATCGC 
ACAGTGTGCG AGGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC 
AGCAAGAAGC TGCGCCCAGG TGGGGCCCGG GGCCCGGCGC CCGCCCCGCC CCGCTGCCCC 
CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG CAGTTGGTGG 
AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG ATTTGCATCG 
ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC TGTGGCGCGG 
CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG ATCTTCGTGT 
AGCCGGCGCG CCCCCCCCCC CCGCCCCTTT CCGGGGCCAT GCCCCGCAGC CGCCCTCGCA 
AGCCCTTTCT CTGTGTTTTA TAAAAAGATT GCTGGACTTT GTGCCTGGTT GCCTGTCTGG 
GGCGGGGCCG TGCAGGTCCC CCAACCGGGG ACCGTTCTGA CTGCACCTCT TACACCGTTG 
GCCCAGCGCT GGGTTCTGCG CTTTGGCCAA AGGCCTTGCA GGGCTTCTTT CCGATCCGTA 
TTCTGCCCTT CTTCCCAGGA CTCACGGGTC CAGAGTGCTG CCCGCACCCT GGCCGGAAAG 
AACGGGGCCC GGGCTTGTGG TGTCGTCCGG GGGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993859.1
CDS	1543..4821
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRPLSPRRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPGGARGPAPAPPRCPQTARRWSARPPRPAHHGSWWRSCRAATGRWRSASPARFASTATSASCSSAAMARARPVARRSAPAPSAASPSAIASRSSCSRRAPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_012138469.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138469.2

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GACCCCTGTC CCCACGCAGG 
GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC 
AGTGCTCCGA ACACTGTGAC CAACCTGCAT GTGGCCACGC CGACGGGGCC TGAGGCCGCT 
GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG CTGGTGCTGT TCTCGCCCTG CCAGCATCGC 
ACAGTGTGCG AGGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC 
AGCAAGAAGC TGCGCCCAGG TGGGGCCCGG GGCCCGGCGC CCGCCCCGCC CCGCTGCCCC 
CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG CAGTTGGTGG 
AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG ATTTGCATCG 
ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC TGTGGCGCGG 
CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG ATCTTCGTGT 
AGCCGGCGCG CCCCCCCCCC CCGCCCCTTT CCGGGGCCAT GCCCCGCAGC CGCCCTCGCA 
AGCCCTTTCT CTGTGTTTTA TAAAAAGATT GCTGGACTTT GTGCCTGGTT GCCTGTCTGG 
GGCGGGGCCG TGCAGGTCCC CCAACCGGGG ACCGTTCTGA CTGCACCTCT TACACCGTTG 
GCCCAGCGCT GGGTTCTGCG CTTTGGCCAA AGGCCTTGCA GGGCTTCTTT CCGATCCGTA 
TTCTGCCCTT CTTCCCAGGA CTCACGGGTC CAGAGTGCTG CCCGCACCCT GGCCGGAAAG 
AACGGGGCCC GGGCTTGTGG TGTCGTCCGG GGGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138471.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993861.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOv18826
Clone ID Related Accession (Same CDS sequence)	XM_012138473.2 , XM_012138476.2 , XM_012138469.2 , XM_012138471.2 , XM_012138474.2 , XM_012138475.2
Accession Version	XM_012138471.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138471.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GACCCCTGTC CCCACGCAGG 
GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC 
AGTGCTCCGA ACACTGTGAC CAACCTGCAT GTGGCCACGC CGACGGGGCC TGAGGCCGCT 
GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG CTGGTGCTGT TCTCGCCCTG CCAGCATCGC 
ACAGTGTGCG AGGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC 
AGCAAGAAGC TGCGCCCAGG TGGGGCCCGG GGCCCGGCGC CCGCCCCGCC CCGCTGCCCC 
CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG CAGTTGGTGG 
AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG ATTTGCATCG 
ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC TGTGGCGCGG 
CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG ATCTTCGTGT 
AGCCGGCGCG CCCCCCCCCC CCGCCCCTTT CCGGGGCCAT GCCCCGCAGC CGCCCTCGCA 
AGCCCTTTCT CTGTGTTTTA TAAAAAGATT GCTGGACTTT GTGCCTGGTT GCCTGTCTGG 
GGCGGGGCCG TGCAGGTCCC CCAACCGGGG ACCGTTCTGA CTGCACCTCT TACACCGTTG 
GCCCAGCGCT GGGTTCTGCG CTTTGGCCAA AGGCCTTGCA GGGCTTCTTT CCGATCCGTA 
TTCTGCCCTT CTTCCCAGGA CTCACGGGTC CAGAGTGCTG CCCGCACCCT GGCCGGAAAG 
AACGGGGCCC GGGCTTGTGG TGTCGTCCGG GGGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993861.1
CDS	1060..4338
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRPLSPRRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPGGARGPAPAPPRCPQTARRWSARPPRPAHHGSWWRSCRAATGRWRSASPARFASTATSASCSSAAMARARPVARRSAPAPSAASPSAIASRSSCSRRAPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_012138471.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138471.2

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GACCCCTGTC CCCACGCAGG 
GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC 
AGTGCTCCGA ACACTGTGAC CAACCTGCAT GTGGCCACGC CGACGGGGCC TGAGGCCGCT 
GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG CTGGTGCTGT TCTCGCCCTG CCAGCATCGC 
ACAGTGTGCG AGGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC 
AGCAAGAAGC TGCGCCCAGG TGGGGCCCGG GGCCCGGCGC CCGCCCCGCC CCGCTGCCCC 
CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG CAGTTGGTGG 
AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG ATTTGCATCG 
ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC TGTGGCGCGG 
CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG ATCTTCGTGT 
AGCCGGCGCG CCCCCCCCCC CCGCCCCTTT CCGGGGCCAT GCCCCGCAGC CGCCCTCGCA 
AGCCCTTTCT CTGTGTTTTA TAAAAAGATT GCTGGACTTT GTGCCTGGTT GCCTGTCTGG 
GGCGGGGCCG TGCAGGTCCC CCAACCGGGG ACCGTTCTGA CTGCACCTCT TACACCGTTG 
GCCCAGCGCT GGGTTCTGCG CTTTGGCCAA AGGCCTTGCA GGGCTTCTTT CCGATCCGTA 
TTCTGCCCTT CTTCCCAGGA CTCACGGGTC CAGAGTGCTG CCCGCACCCT GGCCGGAAAG 
AACGGGGCCC GGGCTTGTGG TGTCGTCCGG GGGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138474.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993864.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OOv18826
Clone ID Related Accession (Same CDS sequence)	XM_012138473.2 , XM_012138476.2 , XM_012138469.2 , XM_012138471.2 , XM_012138474.2 , XM_012138475.2
Accession Version	XM_012138474.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138474.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GACCCCTGTC CCCACGCAGG 
GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC 
AGTGCTCCGA ACACTGTGAC CAACCTGCAT GTGGCCACGC CGACGGGGCC TGAGGCCGCT 
GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG CTGGTGCTGT TCTCGCCCTG CCAGCATCGC 
ACAGTGTGCG AGGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC 
AGCAAGAAGC TGCGCCCAGG TGGGGCCCGG GGCCCGGCGC CCGCCCCGCC CCGCTGCCCC 
CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG CAGTTGGTGG 
AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG ATTTGCATCG 
ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC TGTGGCGCGG 
CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG ATCTTCGTGT 
AGCCGGCGCG CCCCCCCCCC CCGCCCCTTT CCGGGGCCAT GCCCCGCAGC CGCCCTCGCA 
AGCCCTTTCT CTGTGTTTTA TAAAAAGATT GCTGGACTTT GTGCCTGGTT GCCTGTCTGG 
GGCGGGGCCG TGCAGGTCCC CCAACCGGGG ACCGTTCTGA CTGCACCTCT TACACCGTTG 
GCCCAGCGCT GGGTTCTGCG CTTTGGCCAA AGGCCTTGCA GGGCTTCTTT CCGATCCGTA 
TTCTGCCCTT CTTCCCAGGA CTCACGGGTC CAGAGTGCTG CCCGCACCCT GGCCGGAAAG 
AACGGGGCCC GGGCTTGTGG TGTCGTCCGG GGGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993864.1
CDS	993..4271
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRPLSPRRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPGGARGPAPAPPRCPQTARRWSARPPRPAHHGSWWRSCRAATGRWRSASPARFASTATSASCSSAAMARARPVARRSAPAPSAASPSAIASRSSCSRRAPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_012138474.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138474.2

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GACCCCTGTC CCCACGCAGG 
GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC 
AGTGCTCCGA ACACTGTGAC CAACCTGCAT GTGGCCACGC CGACGGGGCC TGAGGCCGCT 
GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG CTGGTGCTGT TCTCGCCCTG CCAGCATCGC 
ACAGTGTGCG AGGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC 
AGCAAGAAGC TGCGCCCAGG TGGGGCCCGG GGCCCGGCGC CCGCCCCGCC CCGCTGCCCC 
CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG CAGTTGGTGG 
AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG ATTTGCATCG 
ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC TGTGGCGCGG 
CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG ATCTTCGTGT 
AGCCGGCGCG CCCCCCCCCC CCGCCCCTTT CCGGGGCCAT GCCCCGCAGC CGCCCTCGCA 
AGCCCTTTCT CTGTGTTTTA TAAAAAGATT GCTGGACTTT GTGCCTGGTT GCCTGTCTGG 
GGCGGGGCCG TGCAGGTCCC CCAACCGGGG ACCGTTCTGA CTGCACCTCT TACACCGTTG 
GCCCAGCGCT GGGTTCTGCG CTTTGGCCAA AGGCCTTGCA GGGCTTCTTT CCGATCCGTA 
TTCTGCCCTT CTTCCCAGGA CTCACGGGTC CAGAGTGCTG CCCGCACCCT GGCCGGAAAG 
AACGGGGCCC GGGCTTGTGG TGTCGTCCGG GGGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138475.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993865.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.

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CloneID	OOv18826
Clone ID Related Accession (Same CDS sequence)	XM_012138473.2 , XM_012138476.2 , XM_012138469.2 , XM_012138471.2 , XM_012138474.2 , XM_012138475.2
Accession Version	XM_012138475.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GACCCCTGTC CCCACGCAGG 
GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC 
AGTGCTCCGA ACACTGTGAC CAACCTGCAT GTGGCCACGC CGACGGGGCC TGAGGCCGCT 
GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG CTGGTGCTGT TCTCGCCCTG CCAGCATCGC 
ACAGTGTGCG AGGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC 
AGCAAGAAGC TGCGCCCAGG TGGGGCCCGG GGCCCGGCGC CCGCCCCGCC CCGCTGCCCC 
CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG CAGTTGGTGG 
AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG ATTTGCATCG 
ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC TGTGGCGCGG 
CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG ATCTTCGTGT 
AGCCGGCGCG CCCCCCCCCC CCGCCCCTTT CCGGGGCCAT GCCCCGCAGC CGCCCTCGCA 
AGCCCTTTCT CTGTGTTTTA TAAAAAGATT GCTGGACTTT GTGCCTGGTT GCCTGTCTGG 
GGCGGGGCCG TGCAGGTCCC CCAACCGGGG ACCGTTCTGA CTGCACCTCT TACACCGTTG 
GCCCAGCGCT GGGTTCTGCG CTTTGGCCAA AGGCCTTGCA GGGCTTCTTT CCGATCCGTA 
TTCTGCCCTT CTTCCCAGGA CTCACGGGTC CAGAGTGCTG CCCGCACCCT GGCCGGAAAG 
AACGGGGCCC GGGCTTGTGG TGTCGTCCGG GGGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993865.1
CDS	783..4061
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRPLSPRRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPGGARGPAPAPPRCPQTARRWSARPPRPAHHGSWWRSCRAATGRWRSASPARFASTATSASCSSAAMARARPVARRSAPAPSAASPSAIASRSSCSRRAPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_012138475.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138475.2

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GACCCCTGTC CCCACGCAGG 
GAGCGGCAGG CTTGCGGCGG TGGCAGCGGG GGCTCGGCCC AGGGCCCAAG ACTGGTGCTC 
AGTGCTCCGA ACACTGTGAC CAACCTGCAT GTGGCCACGC CGACGGGGCC TGAGGCCGCT 
GAGTGCCTAG TGTGCTCAGA GCTGGCGCTG CTGGTGCTGT TCTCGCCCTG CCAGCATCGC 
ACAGTGTGCG AGGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC 
AGCAAGAAGC TGCGCCCAGG TGGGGCCCGG GGCCCGGCGC CCGCCCCGCC CCGCTGCCCC 
CAGACGGCAC GGAGGTGGTC AGCGCGGCCC CCGCGCCCGG CCCACCACGG CAGTTGGTGG 
AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAGCGCAT CACCTGCCCG ATTTGCATCG 
ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCATGGCGC GTGCGCGCCC TGTGGCGCGG 
CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA TCGCATCCAG ATCTTCGTGT 
AGCCGGCGCG CCCCCCCCCC CCGCCCCTTT CCGGGGCCAT GCCCCGCAGC CGCCCTCGCA 
AGCCCTTTCT CTGTGTTTTA TAAAAAGATT GCTGGACTTT GTGCCTGGTT GCCTGTCTGG 
GGCGGGGCCG TGCAGGTCCC CCAACCGGGG ACCGTTCTGA CTGCACCTCT TACACCGTTG 
GCCCAGCGCT GGGTTCTGCG CTTTGGCCAA AGGCCTTGCA GGGCTTCTTT CCGATCCGTA 
TTCTGCCCTT CTTCCCAGGA CTCACGGGTC CAGAGTGCTG CCCGCACCCT GGCCGGAAAG 
AACGGGGCCC GGGCTTGTGG TGTCGTCCGG GGGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138477.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993867.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.

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CloneID	OOv18827
Clone ID Related Accession (Same CDS sequence)	XM_012138477.2
Accession Version	XM_012138477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138477.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAGCCA CGAGACCCGC TGGACCTTCC ATCCTGGGGC TCTCACCAAG 
CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ACGACCTTGA CACGGTGAAG 
CGGCTGCAGG CTGGCCATGG GGAGTGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT 
GGGAGAGTGC TGAAGGTTTT TGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG 
TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC 
GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGTG TTGTCCTGGA CAAGCTTCGA 
GCCCAGAAGA GTGACCTGGA GCACCCAGGG AGGTTGGTGG TGGAGGTGGC TCTGGGCAAC 
ATGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG 
GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGTCAGG TGGAGCTGGT GCGGCTGCTG 
CTGCAGGCTC AGGCGGGCGT TGACCTGCCA GATGACGAGG GCAACACGGC ACTGCACTAC 
GCCGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCGGG TTGTGGGGCC 
AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CTGTGCAAAG GGGCTTCCTG 
GAGGTGGTGA AGGTCCTCTG TGAGCGTGGC TGTGATGTCA ACCTGCCTGA TGCCCATGCT 
GACACACCCC TGCACTGCGC CATCTCGGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC 
CTCACCGAGG TGCCAGGCAT CGACGTCACT GCCACCAACA GCCAGGGCTT CACCCTGCTG 
CACCATGCAT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC GCGGGCCCGG 
CAGCTGGTGG ACGCCAAGAA GGAGGATGGG TTCACGGCAC TGCACCTGGC CGCCTTCAAC 
AACCACGGGG AGGTGGCACA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC 
AACCGTAAGC TGCAGTCCCC GCTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG 
CCACTGCTGG TGGACGCCGG CTGCAGCGTC AACGCCGAGG ACGAGGAGGG GGACACGGCT 
CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG TAGCTGATGG GGGTGCAGGG 
GACCCGGGTC CCCTGCAGTT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC AGGCAGCGCG 
GAGCTGACGG CGGGCGCAGC GATAGCCTAC TTCTTAGCGC TGGAGGGCGC TGACCTGAGC 
TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA 
GCCCTGCAGG GCTGTGCTCA GCGCTTCCGA CCCCTGTCCC CACGCAGGGA GCGGCAGGCT 
TGCGGCGGTG GCAGCGGGGG CTCGGCCCAG GGCCCAAGAC TGGTGCTCAG TGCTCCGAAC 
ACTGTGACCA ACCTGCATGT GGCCACGCCG ACGGGGCCTG AGGCCGCTGA GTGCCTAGTG 
TGCTCAGAGC TGGCGCTGCT GGTGCTGTTC TCGCCCTGCC AGCATCGCAC AGTGTGCGAG 
GAGTGCGCGC GCAGGATGAA GAAATGCATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTG 
CGCCCAGGTG GGGCCCGGGG CCCGGCGCCC GCCCCGCCCC GCTGCCCCCA GACGGCACGG 
AGGTGGTCAG CGCGGCCCCC GCGCCCGGCC CACCACGGCA GTTGGTGGAG GAGCTGCAGA 
GCCGCTACCG GCAGATGGAG GAGCGCATCA CCTGCCCGAT TTGCATCGAC AGCCACATCC 
GCCTCGTGTT CCAGTGCGGC CATGGCGCGT GCGCGCCCTG TGGCGCGGCG CTCAGCGCCT 
GCCCCATCTG CCGCCAGCCC ATCCGCGATC GCATCCAGAT CTTCGTGTAG CCGGCGCGCC 
CCCCCCCCCC GCCCCTTTCC GGGGCCATGC CCCGCAGCCG CCCTCGCAAG CCCTTTCTCT 
GTGTTTTATA AAAAGATTGC TGGACTTTGT GCCTGGTTGC CTGTCTGGGG CGGGGCCGTG 
CAGGTCCCCC AACCGGGGAC CGTTCTGACT GCACCTCTTA CACCGTTGGC CCAGCGCTGG 
GTTCTGCGCT TTGGCCAAAG GCCTTGCAGG GCTTCTTTCC GATCCGTATT CTGCCCTTCT 
TCCCAGGACT CACGGGTCCA GAGTGCTGCC CGCACCCTGG CCGGAAAGAA CGGGGCCCGG 
GCTTGTGGTG TCGTCCGGGG GTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993867.1
CDS	2186..5452
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRPLSPRRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPGGARGPAPAPPRCPQTARRWSARPPRPAHHGSWWRSCRAATGRWRSASPARFASTATSASCSSAAMARARPVARRSAPAPSAASPSAIASRSSCSRRAPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_012138477.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138477.2

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAGCCA CGAGACCCGC TGGACCTTCC ATCCTGGGGC TCTCACCAAG 
CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ACGACCTTGA CACGGTGAAG 
CGGCTGCAGG CTGGCCATGG GGAGTGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT 
GGGAGAGTGC TGAAGGTTTT TGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG 
TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC 
GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGTG TTGTCCTGGA CAAGCTTCGA 
GCCCAGAAGA GTGACCTGGA GCACCCAGGG AGGTTGGTGG TGGAGGTGGC TCTGGGCAAC 
ATGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG 
GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGTCAGG TGGAGCTGGT GCGGCTGCTG 
CTGCAGGCTC AGGCGGGCGT TGACCTGCCA GATGACGAGG GCAACACGGC ACTGCACTAC 
GCCGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCGGG TTGTGGGGCC 
AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CTGTGCAAAG GGGCTTCCTG 
GAGGTGGTGA AGGTCCTCTG TGAGCGTGGC TGTGATGTCA ACCTGCCTGA TGCCCATGCT 
GACACACCCC TGCACTGCGC CATCTCGGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC 
CTCACCGAGG TGCCAGGCAT CGACGTCACT GCCACCAACA GCCAGGGCTT CACCCTGCTG 
CACCATGCAT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC GCGGGCCCGG 
CAGCTGGTGG ACGCCAAGAA GGAGGATGGG TTCACGGCAC TGCACCTGGC CGCCTTCAAC 
AACCACGGGG AGGTGGCACA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC 
AACCGTAAGC TGCAGTCCCC GCTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG 
CCACTGCTGG TGGACGCCGG CTGCAGCGTC AACGCCGAGG ACGAGGAGGG GGACACGGCT 
CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG TAGCTGATGG GGGTGCAGGG 
GACCCGGGTC CCCTGCAGTT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC AGGCAGCGCG 
GAGCTGACGG CGGGCGCAGC GATAGCCTAC TTCTTAGCGC TGGAGGGCGC TGACCTGAGC 
TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA 
GCCCTGCAGG GCTGTGCTCA GCGCTTCCGA CCCCTGTCCC CACGCAGGGA GCGGCAGGCT 
TGCGGCGGTG GCAGCGGGGG CTCGGCCCAG GGCCCAAGAC TGGTGCTCAG TGCTCCGAAC 
ACTGTGACCA ACCTGCATGT GGCCACGCCG ACGGGGCCTG AGGCCGCTGA GTGCCTAGTG 
TGCTCAGAGC TGGCGCTGCT GGTGCTGTTC TCGCCCTGCC AGCATCGCAC AGTGTGCGAG 
GAGTGCGCGC GCAGGATGAA GAAATGCATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTG 
CGCCCAGGTG GGGCCCGGGG CCCGGCGCCC GCCCCGCCCC GCTGCCCCCA GACGGCACGG 
AGGTGGTCAG CGCGGCCCCC GCGCCCGGCC CACCACGGCA GTTGGTGGAG GAGCTGCAGA 
GCCGCTACCG GCAGATGGAG GAGCGCATCA CCTGCCCGAT TTGCATCGAC AGCCACATCC 
GCCTCGTGTT CCAGTGCGGC CATGGCGCGT GCGCGCCCTG TGGCGCGGCG CTCAGCGCCT 
GCCCCATCTG CCGCCAGCCC ATCCGCGATC GCATCCAGAT CTTCGTGTAG CCGGCGCGCC 
CCCCCCCCCC GCCCCTTTCC GGGGCCATGC CCCGCAGCCG CCCTCGCAAG CCCTTTCTCT 
GTGTTTTATA AAAAGATTGC TGGACTTTGT GCCTGGTTGC CTGTCTGGGG CGGGGCCGTG 
CAGGTCCCCC AACCGGGGAC CGTTCTGACT GCACCTCTTA CACCGTTGGC CCAGCGCTGG 
GTTCTGCGCT TTGGCCAAAG GCCTTGCAGG GCTTCTTTCC GATCCGTATT CTGCCCTTCT 
TCCCAGGACT CACGGGTCCA GAGTGCTGCC CGCACCCTGG CCGGAAAGAA CGGGGCCCGG 
GCTTGTGGTG TCGTCCGGGG GTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138478.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993868.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.

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CloneID	OOv18828
Clone ID Related Accession (Same CDS sequence)	XM_012138478.2
Accession Version	XM_012138478.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138478.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTTGCGGC 
GGTGGCAGCG GGGGCTCGGC CCAGGGCCCA AGACTGGTGC TCAGTGCTCC GAACACTGTG 
ACCAACCTGC ATGTGGCCAC GCCGACGGGG CCTGAGGCCG CTGAGTGCCT AGTGTGCTCA 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC 
GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA 
GGTGGGGCCC GGGGCCCGGC GCCCGCCCCG CCCCGCTGCC CCCAGACGGC ACGGAGGTGG 
TCAGCGCGGC CCCCGCGCCC GGCCCACCAC GGCAGTTGGT GGAGGAGCTG CAGAGCCGCT 
ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CGATTTGCAT CGACAGCCAC ATCCGCCTCG 
TGTTCCAGTG CGGCCATGGC GCGTGCGCGC CCTGTGGCGC GGCGCTCAGC GCCTGCCCCA 
TCTGCCGCCA GCCCATCCGC GATCGCATCC AGATCTTCGT GTAGCCGGCG CGCCCCCCCC 
CCCCGCCCCT TTCCGGGGCC ATGCCCCGCA GCCGCCCTCG CAAGCCCTTT CTCTGTGTTT 
TATAAAAAGA TTGCTGGACT TTGTGCCTGG TTGCCTGTCT GGGGCGGGGC CGTGCAGGTC 
CCCCAACCGG GGACCGTTCT GACTGCACCT CTTACACCGT TGGCCCAGCG CTGGGTTCTG 
CGCTTTGGCC AAAGGCCTTG CAGGGCTTCT TTCCGATCCG TATTCTGCCC TTCTTCCCAG 
GACTCACGGG TCCAGAGTGC TGCCCGCACC CTGGCCGGAA AGAACGGGGC CCGGGCTTGT 
GGTGTCGTCC GGGGGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993868.1
CDS	2184..5444
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPGGARGPAPAPPRCPQTARRWSARPPRPAHHGSWWRSCRAATGRWRSASPARFASTATSASCSSAAMARARPVARRSAPAPSAASPSAIASRSSCSRRAPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_012138478.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138478.2

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACGAGACCC GCTGGACCTT CCATCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGACGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGTGGA CCGATGACAT GGCCCCTGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTTGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT 
GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG TGTTGTCCTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGGTTGGT GGTGGAGGTG 
GCTCTGGGCA ACATGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGTCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CAGATGACGA GGGCAACACG 
GCACTGCACT ACGCCGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCG 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCTGTGCAA 
AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAGCGTG GCTGTGATGT CAACCTGCCT 
GATGCCCATG CTGACACACC CCTGCACTGC GCCATCTCGG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGACGTCA CTGCCACCAA CAGCCAGGGC 
TTCACCCTGC TGCACCATGC ATCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCCC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GGTTCACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCA CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGACGCC GGCTGCAGCG TCAACGCCGA GGACGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGTAGCTGAT 
GGGGGTGCAG GGGACCCGGG TCCCCTGCAG TTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCAGGCAGCG CGGAGCTGAC GGCGGGCGCA GCGATAGCCT ACTTCTTAGC GCTGGAGGGC 
GCTGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTTGCGGC 
GGTGGCAGCG GGGGCTCGGC CCAGGGCCCA AGACTGGTGC TCAGTGCTCC GAACACTGTG 
ACCAACCTGC ATGTGGCCAC GCCGACGGGG CCTGAGGCCG CTGAGTGCCT AGTGTGCTCA 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC 
GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA 
GGTGGGGCCC GGGGCCCGGC GCCCGCCCCG CCCCGCTGCC CCCAGACGGC ACGGAGGTGG 
TCAGCGCGGC CCCCGCGCCC GGCCCACCAC GGCAGTTGGT GGAGGAGCTG CAGAGCCGCT 
ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CGATTTGCAT CGACAGCCAC ATCCGCCTCG 
TGTTCCAGTG CGGCCATGGC GCGTGCGCGC CCTGTGGCGC GGCGCTCAGC GCCTGCCCCA 
TCTGCCGCCA GCCCATCCGC GATCGCATCC AGATCTTCGT GTAGCCGGCG CGCCCCCCCC 
CCCCGCCCCT TTCCGGGGCC ATGCCCCGCA GCCGCCCTCG CAAGCCCTTT CTCTGTGTTT 
TATAAAAAGA TTGCTGGACT TTGTGCCTGG TTGCCTGTCT GGGGCGGGGC CGTGCAGGTC 
CCCCAACCGG GGACCGTTCT GACTGCACCT CTTACACCGT TGGCCCAGCG CTGGGTTCTG 
CGCTTTGGCC AAAGGCCTTG CAGGGCTTCT TTCCGATCCG TATTCTGCCC TTCTTCCCAG 
GACTCACGGG TCCAGAGTGC TGCCCGCACC CTGGCCGGAA AGAACGGGGC CCGGGCTTGT 
GGTGTCGTCC GGGGGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138479.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993869.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OOv18829
Clone ID Related Accession (Same CDS sequence)	XM_012138479.2
Accession Version	XM_012138479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT 
ATCGACGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAGTG GACCGATGAC 
ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTTGGAGA CGGGAACGTG 
GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG 
AGTGTTGTCC TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAGGTTG 
GTGGTGGAGG TGGCTCTGGG CAACATGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA 
GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGT 
CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCAGATGAC 
GAGGGCAACA CGGCACTGCA CTACGCCGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG 
CTCTTGAGCT CGGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT 
GTGGCTGTGC AAAGGGGCTT CCTGGAGGTG GTGAAGGTCC TCTGTGAGCG TGGCTGTGAT 
GTCAACCTGC CTGATGCCCA TGCTGACACA CCCCTGCACT GCGCCATCTC GGCGGGCACT 
GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGACGT CACTGCCACC 
AACAGCCAGG GCTTCACCCT GCTGCACCAT GCATCCCTCA AAGGCCACAC ACTAGCTGTC 
AGGAGGATTC TGGCGCGGGC CCGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGGTTCACG 
GCACTGCACC TGGCCGCCTT CAACAACCAC GGGGAGGTGG CACAGGTTCT CATCCAAGAG 
GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCGCTGCA CCTGGCTGTG 
CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGACG CCGGCTGCAG CGTCAACGCC 
GAGGACGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT 
CTGGTAGCTG ATGGGGGTGC AGGGGACCCG GGTCCCCTGC AGTTGCTGTC CAGGCTACAG 
GCCTCGGGCC TTCCAGGCAG CGCGGAGCTG ACGGCGGGCG CAGCGATAGC CTACTTCTTA 
GCGCTGGAGG GCGCTGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG 
GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGACCCCTG 
TCCCCACGCA GGGAGCGGCA GGCTTGCGGC GGTGGCAGCG GGGGCTCGGC CCAGGGCCCA 
AGACTGGTGC TCAGTGCTCC GAACACTGTG ACCAACCTGC ATGTGGCCAC GCCGACGGGG 
CCTGAGGCCG CTGAGTGCCT AGTGTGCTCA GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC 
TGCCAGCATC GCACAGTGTG CGAGGAGTGC GCGCGCAGGA TGAAGAAATG CATCAGGTGC 
CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA GGTGGGGCCC GGGGCCCGGC GCCCGCCCCG 
CCCCGCTGCC CCCAGACGGC ACGGAGGTGG TCAGCGCGGC CCCCGCGCCC GGCCCACCAC 
GGCAGTTGGT GGAGGAGCTG CAGAGCCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC 
CGATTTGCAT CGACAGCCAC ATCCGCCTCG TGTTCCAGTG CGGCCATGGC GCGTGCGCGC 
CCTGTGGCGC GGCGCTCAGC GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GATCGCATCC 
AGATCTTCGT GTAGCCGGCG CGCCCCCCCC CCCCGCCCCT TTCCGGGGCC ATGCCCCGCA 
GCCGCCCTCG CAAGCCCTTT CTCTGTGTTT TATAAAAAGA TTGCTGGACT TTGTGCCTGG 
TTGCCTGTCT GGGGCGGGGC CGTGCAGGTC CCCCAACCGG GGACCGTTCT GACTGCACCT 
CTTACACCGT TGGCCCAGCG CTGGGTTCTG CGCTTTGGCC AAAGGCCTTG CAGGGCTTCT 
TTCCGATCCG TATTCTGCCC TTCTTCCCAG GACTCACGGG TCCAGAGTGC TGCCCGCACC 
CTGGCCGGAA AGAACGGGGC CCGGGCTTGT GGTGTCGTCC GGGGGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993869.1
CDS	2185..5355
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGCVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRPLSPRRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPGGARGPAPAPPRCPQTARRWSARPPRPAHHGSWWRSCRAATGRWRSASPARFASTATSASCSSAAMARARPVARRSAPAPSAASPSAIASRSSCSRRAPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_012138479.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138479.2

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGTGGCGAG GGCTGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACAGTGGTCG TGCAGTGGGA CCACGGCACC 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAATGCCG TATCTGCTTC GACTACGATC TCTGCACGCA GTGCTACATG 
CACAACAAGC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGT 
GTGACATGGG CTGATGGCAC CACCAACGTG TATCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGAGCACCT CCCAAGGCTT 
GGCAAGCCTG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAC GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT 
ATCGACGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAGTG GACCGATGAC 
ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTTGGAGA CGGGAACGTG 
GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG 
AGTGTTGTCC TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAGGTTG 
GTGGTGGAGG TGGCTCTGGG CAACATGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA 
GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGT 
CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCAGATGAC 
GAGGGCAACA CGGCACTGCA CTACGCCGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG 
CTCTTGAGCT CGGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT 
GTGGCTGTGC AAAGGGGCTT CCTGGAGGTG GTGAAGGTCC TCTGTGAGCG TGGCTGTGAT 
GTCAACCTGC CTGATGCCCA TGCTGACACA CCCCTGCACT GCGCCATCTC GGCGGGCACT 
GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGACGT CACTGCCACC 
AACAGCCAGG GCTTCACCCT GCTGCACCAT GCATCCCTCA AAGGCCACAC ACTAGCTGTC 
AGGAGGATTC TGGCGCGGGC CCGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGGTTCACG 
GCACTGCACC TGGCCGCCTT CAACAACCAC GGGGAGGTGG CACAGGTTCT CATCCAAGAG 
GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCGCTGCA CCTGGCTGTG 
CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGACG CCGGCTGCAG CGTCAACGCC 
GAGGACGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT 
CTGGTAGCTG ATGGGGGTGC AGGGGACCCG GGTCCCCTGC AGTTGCTGTC CAGGCTACAG 
GCCTCGGGCC TTCCAGGCAG CGCGGAGCTG ACGGCGGGCG CAGCGATAGC CTACTTCTTA 
GCGCTGGAGG GCGCTGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG 
GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGACCCCTG 
TCCCCACGCA GGGAGCGGCA GGCTTGCGGC GGTGGCAGCG GGGGCTCGGC CCAGGGCCCA 
AGACTGGTGC TCAGTGCTCC GAACACTGTG ACCAACCTGC ATGTGGCCAC GCCGACGGGG 
CCTGAGGCCG CTGAGTGCCT AGTGTGCTCA GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC 
TGCCAGCATC GCACAGTGTG CGAGGAGTGC GCGCGCAGGA TGAAGAAATG CATCAGGTGC 
CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA GGTGGGGCCC GGGGCCCGGC GCCCGCCCCG 
CCCCGCTGCC CCCAGACGGC ACGGAGGTGG TCAGCGCGGC CCCCGCGCCC GGCCCACCAC 
GGCAGTTGGT GGAGGAGCTG CAGAGCCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC 
CGATTTGCAT CGACAGCCAC ATCCGCCTCG TGTTCCAGTG CGGCCATGGC GCGTGCGCGC 
CCTGTGGCGC GGCGCTCAGC GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GATCGCATCC 
AGATCTTCGT GTAGCCGGCG CGCCCCCCCC CCCCGCCCCT TTCCGGGGCC ATGCCCCGCA 
GCCGCCCTCG CAAGCCCTTT CTCTGTGTTT TATAAAAAGA TTGCTGGACT TTGTGCCTGG 
TTGCCTGTCT GGGGCGGGGC CGTGCAGGTC CCCCAACCGG GGACCGTTCT GACTGCACCT 
CTTACACCGT TGGCCCAGCG CTGGGTTCTG CGCTTTGGCC AAAGGCCTTG CAGGGCTTCT 
TTCCGATCCG TATTCTGCCC TTCTTCCCAG GACTCACGGG TCCAGAGTGC TGCCCGCACC 
CTGGCCGGAA AGAACGGGGC CCGGGCTTGT GGTGTCGTCC GGGGGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138480.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993870.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OOv18830
Clone ID Related Accession (Same CDS sequence)	XM_012138480.2
Accession Version	XM_012138480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACGC CCAAATCGGT GTCCGCCACC CCAACATCAT CTGCGACTGC TGCAAGAAGC 
ACGGGCTGCG GGGCATGCGC TGGAAATGCC GTATCTGCTT CGACTACGAT CTCTGCACGC 
AGTGCTACAT GCACAACAAG CACGACCTGG CCCACGCCTT CGAGCGCTAC GAGACGGCCC 
ACTCGCGCCC GTCAAGTCTG TCCTGAGCCA TTTCCTGGAG GAGTGACCAG GGTGACGATC 
ACCCCAGGGG CCTGGGCATT GACACCAGCC CCCACCCCCT ACAGAAGGCG ACTGTGTCCA 
CCTAGCAGGG TCATGCTGAG TCCCCGCCAG GGCCTCCCAA GGATCCCGTT AAGGGGCATC 
TTCCAGGGGG CGAAGGTGGT TCGGGGCCCC GACTGGGAGT GGGGCTCACA GGATGGAGGA 
GAAGGGAAGC CAGGCCGAGT AGTGGACATC CGTGGCTGGG ACGTGGAGAC AGGCCGGAGT 
GTGGCCAGTG TGACATGGGC TGATGGCACC ACCAACGTGT ATCGTGTGGG CCACAAGGGC 
AAGGTGGACC TCAAGTGTGT GGGCGAGGCA GCTGGCGGCT TCTACTACAG GGAGCACCTC 
CCAAGGCTTG GCAAGCCTGC AGAGCTGCAG CGCAGGGTCA GTGCTGATGG CCAGCCCTTC 
CAGCGTGGGG ACAAGGTCAA GTGTCTGCTG GACACAGACG TGCTGAGGGA GATGCAGGAA 
GGCCACGGCG GGTGGAACCC CCGGATGGCC AAGTTTATCG GACAGACGGG CACCGTGCAC 
CGCATCACAG ACCGTGGGGA CGTGCGTGTG CAGTTCAGCC ACGAGACCCG CTGGACCTTC 
CATCCTGGGG CTCTCACCAA GCAAAACTCC TGCTTGGTGG GTGAGGTGGT GCGGGTTATC 
GACGACCTTG ACACGGTGAA GCGGCTGCAG GCTGGCCATG GGGAGTGGAC CGATGACATG 
GCCCCTGCTC TGGGCCGCAT TGGGAGAGTG CTGAAGGTTT TTGGAGACGG GAACGTGGGT 
GTGCTGGTCA GTGGGAAGCT GTGGACCTTC AGCCCCTCCT GCCTGGTGGC CTACCGGCCT 
GAGGAAGATG CCAACCTGGA CGTAGCTGGG CGAGCCCGGG AGAACAAAAG CTCCCTGAGT 
GTTGTCCTGG ACAAGCTTCG AGCCCAGAAG AGTGACCTGG AGCACCCAGG GAGGTTGGTG 
GTGGAGGTGG CTCTGGGCAA CATGGCCCGG GCTCTGGACC TGCTGCGGCG GCACCCAGAA 
CAGGTGGACA CCAAGAATCA GGGCAGGACT GCCCTGCAGA TGGCTGCCTA CCTAGGTCAG 
GTGGAGCTGG TGCGGCTGCT GCTGCAGGCT CAGGCGGGCG TTGACCTGCC AGATGACGAG 
GGCAACACGG CACTGCACTA CGCCGCCCTG GGGAACCAGC CTGAGGCTGC CCGGGTGCTC 
TTGAGCTCGG GTTGTGGGGC CAATGCTCTG AACAGCACTC GGAGCTCAGC ACTGCATGTG 
GCTGTGCAAA GGGGCTTCCT GGAGGTGGTG AAGGTCCTCT GTGAGCGTGG CTGTGATGTC 
AACCTGCCTG ATGCCCATGC TGACACACCC CTGCACTGCG CCATCTCGGC GGGCACTGGT 
GCCAGCGGCA TTGTGGAGGT CCTCACCGAG GTGCCAGGCA TCGACGTCAC TGCCACCAAC 
AGCCAGGGCT TCACCCTGCT GCACCATGCA TCCCTCAAAG GCCACACACT AGCTGTCAGG 
AGGATTCTGG CGCGGGCCCG GCAGCTGGTG GACGCCAAGA AGGAGGATGG GTTCACGGCA 
CTGCACCTGG CCGCCTTCAA CAACCACGGG GAGGTGGCAC AGGTTCTCAT CCAAGAGGGC 
CACTGTGATG TGAACGCTCG CAACCGTAAG CTGCAGTCCC CGCTGCACCT GGCTGTGCAG 
CAGGCCCACG TGGGGCTGGT GCCACTGCTG GTGGACGCCG GCTGCAGCGT CAACGCCGAG 
GACGAGGAGG GGGACACGGC TCTGCACGTG GCCCTGCAGC GCCACCAGCT GCTGCCTCTG 
GTAGCTGATG GGGGTGCAGG GGACCCGGGT CCCCTGCAGT TGCTGTCCAG GCTACAGGCC 
TCGGGCCTTC CAGGCAGCGC GGAGCTGACG GCGGGCGCAG CGATAGCCTA CTTCTTAGCG 
CTGGAGGGCG CTGACCTGAG CTACGCCAAC CACCGCGGCC GGAGCCCCCT GGACCTGGCT 
GCCGAGGGTC GCCTGCTCAA AGCCCTGCAG GGCTGTGCTC AGCGCTTCCG ACCCCTGTCC 
CCACGCAGGG AGCGGCAGGC TTGCGGCGGT GGCAGCGGGG GCTCGGCCCA GGGCCCAAGA 
CTGGTGCTCA GTGCTCCGAA CACTGTGACC AACCTGCATG TGGCCACGCC GACGGGGCCT 
GAGGCCGCTG AGTGCCTAGT GTGCTCAGAG CTGGCGCTGC TGGTGCTGTT CTCGCCCTGC 
CAGCATCGCA CAGTGTGCGA GGAGTGCGCG CGCAGGATGA AGAAATGCAT CAGGTGCCAG 
GTGGTCATCA GCAAGAAGCT GCGCCCAGGT GGGGCCCGGG GCCCGGCGCC CGCCCCGCCC 
CGCTGCCCCC AGACGGCACG GAGGTGGTCA GCGCGGCCCC CGCGCCCGGC CCACCACGGC 
AGTTGGTGGA GGAGCTGCAG AGCCGCTACC GGCAGATGGA GGAGCGCATC ACCTGCCCGA 
TTTGCATCGA CAGCCACATC CGCCTCGTGT TCCAGTGCGG CCATGGCGCG TGCGCGCCCT 
GTGGCGCGGC GCTCAGCGCC TGCCCCATCT GCCGCCAGCC CATCCGCGAT CGCATCCAGA 
TCTTCGTGTA GCCGGCGCGC CCCCCCCCCC CGCCCCTTTC CGGGGCCATG CCCCGCAGCC 
GCCCTCGCAA GCCCTTTCTC TGTGTTTTAT AAAAAGATTG CTGGACTTTG TGCCTGGTTG 
CCTGTCTGGG GCGGGGCCGT GCAGGTCCCC CAACCGGGGA CCGTTCTGAC TGCACCTCTT 
ACACCGTTGG CCCAGCGCTG GGTTCTGCGC TTTGGCCAAA GGCCTTGCAG GGCTTCTTTC 
CGATCCGTAT TCTGCCCTTC TTCCCAGGAC TCACGGGTCC AGAGTGCTGC CCGCACCCTG 
GCCGGAAAGA ACGGGGCCCG GGCTTGTGGT GTCGTCCGGG GGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993870.1
CDS	149..3316
Translation	MTTPKSVSATPTSSATAARSTGCGACAGNAVSASTTISARSATCTTSTTWPTPSSATRRPTRARQVCPEPFPGGVTRVTITPGAWALTPAPTPYRRRLCPPSRVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRPLSPRRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPGGARGPAPAPPRCPQTARRWSARPPRPAHHGSWWRSCRAATGRWRSASPARFASTATSASCSSAAMARARPVARRSAPAPSAASPSAIASRSSCSRRAPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_012138480.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138480.2

ORF Insert Sequence:

ATGACAACGC CCAAATCGGT GTCCGCCACC CCAACATCAT CTGCGACTGC TGCAAGAAGC 
ACGGGCTGCG GGGCATGCGC TGGAAATGCC GTATCTGCTT CGACTACGAT CTCTGCACGC 
AGTGCTACAT GCACAACAAG CACGACCTGG CCCACGCCTT CGAGCGCTAC GAGACGGCCC 
ACTCGCGCCC GTCAAGTCTG TCCTGAGCCA TTTCCTGGAG GAGTGACCAG GGTGACGATC 
ACCCCAGGGG CCTGGGCATT GACACCAGCC CCCACCCCCT ACAGAAGGCG ACTGTGTCCA 
CCTAGCAGGG TCATGCTGAG TCCCCGCCAG GGCCTCCCAA GGATCCCGTT AAGGGGCATC 
TTCCAGGGGG CGAAGGTGGT TCGGGGCCCC GACTGGGAGT GGGGCTCACA GGATGGAGGA 
GAAGGGAAGC CAGGCCGAGT AGTGGACATC CGTGGCTGGG ACGTGGAGAC AGGCCGGAGT 
GTGGCCAGTG TGACATGGGC TGATGGCACC ACCAACGTGT ATCGTGTGGG CCACAAGGGC 
AAGGTGGACC TCAAGTGTGT GGGCGAGGCA GCTGGCGGCT TCTACTACAG GGAGCACCTC 
CCAAGGCTTG GCAAGCCTGC AGAGCTGCAG CGCAGGGTCA GTGCTGATGG CCAGCCCTTC 
CAGCGTGGGG ACAAGGTCAA GTGTCTGCTG GACACAGACG TGCTGAGGGA GATGCAGGAA 
GGCCACGGCG GGTGGAACCC CCGGATGGCC AAGTTTATCG GACAGACGGG CACCGTGCAC 
CGCATCACAG ACCGTGGGGA CGTGCGTGTG CAGTTCAGCC ACGAGACCCG CTGGACCTTC 
CATCCTGGGG CTCTCACCAA GCAAAACTCC TGCTTGGTGG GTGAGGTGGT GCGGGTTATC 
GACGACCTTG ACACGGTGAA GCGGCTGCAG GCTGGCCATG GGGAGTGGAC CGATGACATG 
GCCCCTGCTC TGGGCCGCAT TGGGAGAGTG CTGAAGGTTT TTGGAGACGG GAACGTGGGT 
GTGCTGGTCA GTGGGAAGCT GTGGACCTTC AGCCCCTCCT GCCTGGTGGC CTACCGGCCT 
GAGGAAGATG CCAACCTGGA CGTAGCTGGG CGAGCCCGGG AGAACAAAAG CTCCCTGAGT 
GTTGTCCTGG ACAAGCTTCG AGCCCAGAAG AGTGACCTGG AGCACCCAGG GAGGTTGGTG 
GTGGAGGTGG CTCTGGGCAA CATGGCCCGG GCTCTGGACC TGCTGCGGCG GCACCCAGAA 
CAGGTGGACA CCAAGAATCA GGGCAGGACT GCCCTGCAGA TGGCTGCCTA CCTAGGTCAG 
GTGGAGCTGG TGCGGCTGCT GCTGCAGGCT CAGGCGGGCG TTGACCTGCC AGATGACGAG 
GGCAACACGG CACTGCACTA CGCCGCCCTG GGGAACCAGC CTGAGGCTGC CCGGGTGCTC 
TTGAGCTCGG GTTGTGGGGC CAATGCTCTG AACAGCACTC GGAGCTCAGC ACTGCATGTG 
GCTGTGCAAA GGGGCTTCCT GGAGGTGGTG AAGGTCCTCT GTGAGCGTGG CTGTGATGTC 
AACCTGCCTG ATGCCCATGC TGACACACCC CTGCACTGCG CCATCTCGGC GGGCACTGGT 
GCCAGCGGCA TTGTGGAGGT CCTCACCGAG GTGCCAGGCA TCGACGTCAC TGCCACCAAC 
AGCCAGGGCT TCACCCTGCT GCACCATGCA TCCCTCAAAG GCCACACACT AGCTGTCAGG 
AGGATTCTGG CGCGGGCCCG GCAGCTGGTG GACGCCAAGA AGGAGGATGG GTTCACGGCA 
CTGCACCTGG CCGCCTTCAA CAACCACGGG GAGGTGGCAC AGGTTCTCAT CCAAGAGGGC 
CACTGTGATG TGAACGCTCG CAACCGTAAG CTGCAGTCCC CGCTGCACCT GGCTGTGCAG 
CAGGCCCACG TGGGGCTGGT GCCACTGCTG GTGGACGCCG GCTGCAGCGT CAACGCCGAG 
GACGAGGAGG GGGACACGGC TCTGCACGTG GCCCTGCAGC GCCACCAGCT GCTGCCTCTG 
GTAGCTGATG GGGGTGCAGG GGACCCGGGT CCCCTGCAGT TGCTGTCCAG GCTACAGGCC 
TCGGGCCTTC CAGGCAGCGC GGAGCTGACG GCGGGCGCAG CGATAGCCTA CTTCTTAGCG 
CTGGAGGGCG CTGACCTGAG CTACGCCAAC CACCGCGGCC GGAGCCCCCT GGACCTGGCT 
GCCGAGGGTC GCCTGCTCAA AGCCCTGCAG GGCTGTGCTC AGCGCTTCCG ACCCCTGTCC 
CCACGCAGGG AGCGGCAGGC TTGCGGCGGT GGCAGCGGGG GCTCGGCCCA GGGCCCAAGA 
CTGGTGCTCA GTGCTCCGAA CACTGTGACC AACCTGCATG TGGCCACGCC GACGGGGCCT 
GAGGCCGCTG AGTGCCTAGT GTGCTCAGAG CTGGCGCTGC TGGTGCTGTT CTCGCCCTGC 
CAGCATCGCA CAGTGTGCGA GGAGTGCGCG CGCAGGATGA AGAAATGCAT CAGGTGCCAG 
GTGGTCATCA GCAAGAAGCT GCGCCCAGGT GGGGCCCGGG GCCCGGCGCC CGCCCCGCCC 
CGCTGCCCCC AGACGGCACG GAGGTGGTCA GCGCGGCCCC CGCGCCCGGC CCACCACGGC 
AGTTGGTGGA GGAGCTGCAG AGCCGCTACC GGCAGATGGA GGAGCGCATC ACCTGCCCGA 
TTTGCATCGA CAGCCACATC CGCCTCGTGT TCCAGTGCGG CCATGGCGCG TGCGCGCCCT 
GTGGCGCGGC GCTCAGCGCC TGCCCCATCT GCCGCCAGCC CATCCGCGAT CGCATCCAGA 
TCTTCGTGTA GCCGGCGCGC CCCCCCCCCC CGCCCCTTTC CGGGGCCATG CCCCGCAGCC 
GCCCTCGCAA GCCCTTTCTC TGTGTTTTAT AAAAAGATTG CTGGACTTTG TGCCTGGTTG 
CCTGTCTGGG GCGGGGCCGT GCAGGTCCCC CAACCGGGGA CCGTTCTGAC TGCACCTCTT 
ACACCGTTGG CCCAGCGCTG GGTTCTGCGC TTTGGCCAAA GGCCTTGCAG GGCTTCTTTC 
CGATCCGTAT TCTGCCCTTC TTCCCAGGAC TCACGGGTCC AGAGTGCTGC CCGCACCCTG 
GCCGGAAAGA ACGGGGCCCG GGCTTGTGGT GTCGTCCGGG GGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138484.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993874.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OOv18834
Clone ID Related Accession (Same CDS sequence)	XM_012138484.2
Accession Version	XM_012138484.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138484.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTCA TGCTGAGTCC CCGCCAGGGC CTCCCAAGGA TCCCGTTAAG GGGCATCTTC 
CAGGGGGCGA AGGTGGTTCG GGGCCCCGAC TGGGAGTGGG GCTCACAGGA TGGAGGAGAA 
GGGAAGCCAG GCCGAGTAGT GGACATCCGT GGCTGGGACG TGGAGACAGG CCGGAGTGTG 
GCCAGTGTGA CATGGGCTGA TGGCACCACC AACGTGTATC GTGTGGGCCA CAAGGGCAAG 
GTGGACCTCA AGTGTGTGGG CGAGGCAGCT GGCGGCTTCT ACTACAGGGA GCACCTCCCA 
AGGCTTGGCA AGCCTGCAGA GCTGCAGCGC AGGGTCAGTG CTGATGGCCA GCCCTTCCAG 
CGTGGGGACA AGGTCAAGTG TCTGCTGGAC ACAGACGTGC TGAGGGAGAT GCAGGAAGGC 
CACGGCGGGT GGAACCCCCG GATGGCCAAG TTTATCGGAC AGACGGGCAC CGTGCACCGC 
ATCACAGACC GTGGGGACGT GCGTGTGCAG TTCAGCCACG AGACCCGCTG GACCTTCCAT 
CCTGGGGCTC TCACCAAGCA AAACTCCTGC TTGGTGGGTG AGGTGGTGCG GGTTATCGAC 
GACCTTGACA CGGTGAAGCG GCTGCAGGCT GGCCATGGGG AGTGGACCGA TGACATGGCC 
CCTGCTCTGG GCCGCATTGG GAGAGTGCTG AAGGTTTTTG GAGACGGGAA CGTGGGTGTG 
CTGGTCAGTG GGAAGCTGTG GACCTTCAGC CCCTCCTGCC TGGTGGCCTA CCGGCCTGAG 
GAAGATGCCA ACCTGGACGT AGCTGGGCGA GCCCGGGAGA ACAAAAGCTC CCTGAGTGTT 
GTCCTGGACA AGCTTCGAGC CCAGAAGAGT GACCTGGAGC ACCCAGGGAG GTTGGTGGTG 
GAGGTGGCTC TGGGCAACAT GGCCCGGGCT CTGGACCTGC TGCGGCGGCA CCCAGAACAG 
GTGGACACCA AGAATCAGGG CAGGACTGCC CTGCAGATGG CTGCCTACCT AGGTCAGGTG 
GAGCTGGTGC GGCTGCTGCT GCAGGCTCAG GCGGGCGTTG ACCTGCCAGA TGACGAGGGC 
AACACGGCAC TGCACTACGC CGCCCTGGGG AACCAGCCTG AGGCTGCCCG GGTGCTCTTG 
AGCTCGGGTT GTGGGGCCAA TGCTCTGAAC AGCACTCGGA GCTCAGCACT GCATGTGGCT 
GTGCAAAGGG GCTTCCTGGA GGTGGTGAAG GTCCTCTGTG AGCGTGGCTG TGATGTCAAC 
CTGCCTGATG CCCATGCTGA CACACCCCTG CACTGCGCCA TCTCGGCGGG CACTGGTGCC 
AGCGGCATTG TGGAGGTCCT CACCGAGGTG CCAGGCATCG ACGTCACTGC CACCAACAGC 
CAGGGCTTCA CCCTGCTGCA CCATGCATCC CTCAAAGGCC ACACACTAGC TGTCAGGAGG 
ATTCTGGCGC GGGCCCGGCA GCTGGTGGAC GCCAAGAAGG AGGATGGGTT CACGGCACTG 
CACCTGGCCG CCTTCAACAA CCACGGGGAG GTGGCACAGG TTCTCATCCA AGAGGGCCAC 
TGTGATGTGA ACGCTCGCAA CCGTAAGCTG CAGTCCCCGC TGCACCTGGC TGTGCAGCAG 
GCCCACGTGG GGCTGGTGCC ACTGCTGGTG GACGCCGGCT GCAGCGTCAA CGCCGAGGAC 
GAGGAGGGGG ACACGGCTCT GCACGTGGCC CTGCAGCGCC ACCAGCTGCT GCCTCTGGTA 
GCTGATGGGG GTGCAGGGGA CCCGGGTCCC CTGCAGTTGC TGTCCAGGCT ACAGGCCTCG 
GGCCTTCCAG GCAGCGCGGA GCTGACGGCG GGCGCAGCGA TAGCCTACTT CTTAGCGCTG 
GAGGGCGCTG ACCTGAGCTA CGCCAACCAC CGCGGCCGGA GCCCCCTGGA CCTGGCTGCC 
GAGGGTCGCC TGCTCAAAGC CCTGCAGGGC TGTGCTCAGC GCTTCCGACC CCTGTCCCCA 
CGCAGGGAGC GGCAGGCTTG CGGCGGTGGC AGCGGGGGCT CGGCCCAGGG CCCAAGACTG 
GTGCTCAGTG CTCCGAACAC TGTGACCAAC CTGCATGTGG CCACGCCGAC GGGGCCTGAG 
GCCGCTGAGT GCCTAGTGTG CTCAGAGCTG GCGCTGCTGG TGCTGTTCTC GCCCTGCCAG 
CATCGCACAG TGTGCGAGGA GTGCGCGCGC AGGATGAAGA AATGCATCAG GTGCCAGGTG 
GTCATCAGCA AGAAGCTGCG CCCAGGTGGG GCCCGGGGCC CGGCGCCCGC CCCGCCCCGC 
TGCCCCCAGA CGGCACGGAG GTGGTCAGCG CGGCCCCCGC GCCCGGCCCA CCACGGCAGT 
TGGTGGAGGA GCTGCAGAGC CGCTACCGGC AGATGGAGGA GCGCATCACC TGCCCGATTT 
GCATCGACAG CCACATCCGC CTCGTGTTCC AGTGCGGCCA TGGCGCGTGC GCGCCCTGTG 
GCGCGGCGCT CAGCGCCTGC CCCATCTGCC GCCAGCCCAT CCGCGATCGC ATCCAGATCT 
TCGTGTAGCC GGCGCGCCCC CCCCCCCCGC CCCTTTCCGG GGCCATGCCC CGCAGCCGCC 
CTCGCAAGCC CTTTCTCTGT GTTTTATAAA AAGATTGCTG GACTTTGTGC CTGGTTGCCT 
GTCTGGGGCG GGGCCGTGCA GGTCCCCCAA CCGGGGACCG TTCTGACTGC ACCTCTTACA 
CCGTTGGCCC AGCGCTGGGT TCTGCGCTTT GGCCAAAGGC CTTGCAGGGC TTCTTTCCGA 
TCCGTATTCT GCCCTTCTTC CCAGGACTCA CGGGTCCAGA GTGCTGCCCG CACCCTGGCC 
GGAAAGAACG GGGCCCGGGC TTGTGGTGTC GTCCGGGGGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993874.1
CDS	305..3169
Translation	MRVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRPLSPRRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPGGARGPAPAPPRCPQTARRWSARPPRPAHHGSWWRSCRAATGRWRSASPARFASTATSASCSSAAMARARPVARRSAPAPSAASPSAIASRSSCSRRAPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_012138484.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138484.2

ORF Insert Sequence:

ATGCGGGTCA TGCTGAGTCC CCGCCAGGGC CTCCCAAGGA TCCCGTTAAG GGGCATCTTC 
CAGGGGGCGA AGGTGGTTCG GGGCCCCGAC TGGGAGTGGG GCTCACAGGA TGGAGGAGAA 
GGGAAGCCAG GCCGAGTAGT GGACATCCGT GGCTGGGACG TGGAGACAGG CCGGAGTGTG 
GCCAGTGTGA CATGGGCTGA TGGCACCACC AACGTGTATC GTGTGGGCCA CAAGGGCAAG 
GTGGACCTCA AGTGTGTGGG CGAGGCAGCT GGCGGCTTCT ACTACAGGGA GCACCTCCCA 
AGGCTTGGCA AGCCTGCAGA GCTGCAGCGC AGGGTCAGTG CTGATGGCCA GCCCTTCCAG 
CGTGGGGACA AGGTCAAGTG TCTGCTGGAC ACAGACGTGC TGAGGGAGAT GCAGGAAGGC 
CACGGCGGGT GGAACCCCCG GATGGCCAAG TTTATCGGAC AGACGGGCAC CGTGCACCGC 
ATCACAGACC GTGGGGACGT GCGTGTGCAG TTCAGCCACG AGACCCGCTG GACCTTCCAT 
CCTGGGGCTC TCACCAAGCA AAACTCCTGC TTGGTGGGTG AGGTGGTGCG GGTTATCGAC 
GACCTTGACA CGGTGAAGCG GCTGCAGGCT GGCCATGGGG AGTGGACCGA TGACATGGCC 
CCTGCTCTGG GCCGCATTGG GAGAGTGCTG AAGGTTTTTG GAGACGGGAA CGTGGGTGTG 
CTGGTCAGTG GGAAGCTGTG GACCTTCAGC CCCTCCTGCC TGGTGGCCTA CCGGCCTGAG 
GAAGATGCCA ACCTGGACGT AGCTGGGCGA GCCCGGGAGA ACAAAAGCTC CCTGAGTGTT 
GTCCTGGACA AGCTTCGAGC CCAGAAGAGT GACCTGGAGC ACCCAGGGAG GTTGGTGGTG 
GAGGTGGCTC TGGGCAACAT GGCCCGGGCT CTGGACCTGC TGCGGCGGCA CCCAGAACAG 
GTGGACACCA AGAATCAGGG CAGGACTGCC CTGCAGATGG CTGCCTACCT AGGTCAGGTG 
GAGCTGGTGC GGCTGCTGCT GCAGGCTCAG GCGGGCGTTG ACCTGCCAGA TGACGAGGGC 
AACACGGCAC TGCACTACGC CGCCCTGGGG AACCAGCCTG AGGCTGCCCG GGTGCTCTTG 
AGCTCGGGTT GTGGGGCCAA TGCTCTGAAC AGCACTCGGA GCTCAGCACT GCATGTGGCT 
GTGCAAAGGG GCTTCCTGGA GGTGGTGAAG GTCCTCTGTG AGCGTGGCTG TGATGTCAAC 
CTGCCTGATG CCCATGCTGA CACACCCCTG CACTGCGCCA TCTCGGCGGG CACTGGTGCC 
AGCGGCATTG TGGAGGTCCT CACCGAGGTG CCAGGCATCG ACGTCACTGC CACCAACAGC 
CAGGGCTTCA CCCTGCTGCA CCATGCATCC CTCAAAGGCC ACACACTAGC TGTCAGGAGG 
ATTCTGGCGC GGGCCCGGCA GCTGGTGGAC GCCAAGAAGG AGGATGGGTT CACGGCACTG 
CACCTGGCCG CCTTCAACAA CCACGGGGAG GTGGCACAGG TTCTCATCCA AGAGGGCCAC 
TGTGATGTGA ACGCTCGCAA CCGTAAGCTG CAGTCCCCGC TGCACCTGGC TGTGCAGCAG 
GCCCACGTGG GGCTGGTGCC ACTGCTGGTG GACGCCGGCT GCAGCGTCAA CGCCGAGGAC 
GAGGAGGGGG ACACGGCTCT GCACGTGGCC CTGCAGCGCC ACCAGCTGCT GCCTCTGGTA 
GCTGATGGGG GTGCAGGGGA CCCGGGTCCC CTGCAGTTGC TGTCCAGGCT ACAGGCCTCG 
GGCCTTCCAG GCAGCGCGGA GCTGACGGCG GGCGCAGCGA TAGCCTACTT CTTAGCGCTG 
GAGGGCGCTG ACCTGAGCTA CGCCAACCAC CGCGGCCGGA GCCCCCTGGA CCTGGCTGCC 
GAGGGTCGCC TGCTCAAAGC CCTGCAGGGC TGTGCTCAGC GCTTCCGACC CCTGTCCCCA 
CGCAGGGAGC GGCAGGCTTG CGGCGGTGGC AGCGGGGGCT CGGCCCAGGG CCCAAGACTG 
GTGCTCAGTG CTCCGAACAC TGTGACCAAC CTGCATGTGG CCACGCCGAC GGGGCCTGAG 
GCCGCTGAGT GCCTAGTGTG CTCAGAGCTG GCGCTGCTGG TGCTGTTCTC GCCCTGCCAG 
CATCGCACAG TGTGCGAGGA GTGCGCGCGC AGGATGAAGA AATGCATCAG GTGCCAGGTG 
GTCATCAGCA AGAAGCTGCG CCCAGGTGGG GCCCGGGGCC CGGCGCCCGC CCCGCCCCGC 
TGCCCCCAGA CGGCACGGAG GTGGTCAGCG CGGCCCCCGC GCCCGGCCCA CCACGGCAGT 
TGGTGGAGGA GCTGCAGAGC CGCTACCGGC AGATGGAGGA GCGCATCACC TGCCCGATTT 
GCATCGACAG CCACATCCGC CTCGTGTTCC AGTGCGGCCA TGGCGCGTGC GCGCCCTGTG 
GCGCGGCGCT CAGCGCCTGC CCCATCTGCC GCCAGCCCAT CCGCGATCGC ATCCAGATCT 
TCGTGTAGCC GGCGCGCCCC CCCCCCCCGC CCCTTTCCGG GGCCATGCCC CGCAGCCGCC 
CTCGCAAGCC CTTTCTCTGT GTTTTATAAA AAGATTGCTG GACTTTGTGC CTGGTTGCCT 
GTCTGGGGCG GGGCCGTGCA GGTCCCCCAA CCGGGGACCG TTCTGACTGC ACCTCTTACA 
CCGTTGGCCC AGCGCTGGGT TCTGCGCTTT GGCCAAAGGC CTTGCAGGGC TTCTTTCCGA 
TCCGTATTCT GCCCTTCTTC CCAGGACTCA CGGGTCCAGA GTGCTGCCCG CACCCTGGCC 
GGAAAGAACG GGGCCCGGGC TTGTGGTGTC GTCCGGGGGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_012138485.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011993875.1 Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OOv18835
Clone ID Related Accession (Same CDS sequence)	XM_012138485.2
Accession Version	XM_012138485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase MIB2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTC CCCGCCAGGG CCTCCCAAGG ATCCCGTTAA GGGGCATCTT CCAGGGGGCG 
AAGGTGGTTC GGGGCCCCGA CTGGGAGTGG GGCTCACAGG ATGGAGGAGA AGGGAAGCCA 
GGCCGAGTAG TGGACATCCG TGGCTGGGAC GTGGAGACAG GCCGGAGTGT GGCCAGTGTG 
ACATGGGCTG ATGGCACCAC CAACGTGTAT CGTGTGGGCC ACAAGGGCAA GGTGGACCTC 
AAGTGTGTGG GCGAGGCAGC TGGCGGCTTC TACTACAGGG AGCACCTCCC AAGGCTTGGC 
AAGCCTGCAG AGCTGCAGCG CAGGGTCAGT GCTGATGGCC AGCCCTTCCA GCGTGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACAGACGTG CTGAGGGAGA TGCAGGAAGG CCACGGCGGG 
TGGAACCCCC GGATGGCCAA GTTTATCGGA CAGACGGGCA CCGTGCACCG CATCACAGAC 
CGTGGGGACG TGCGTGTGCA GTTCAGCCAC GAGACCCGCT GGACCTTCCA TCCTGGGGCT 
CTCACCAAGC AAAACTCCTG CTTGGTGGGT GAGGTGGTGC GGGTTATCGA CGACCTTGAC 
ACGGTGAAGC GGCTGCAGGC TGGCCATGGG GAGTGGACCG ATGACATGGC CCCTGCTCTG 
GGCCGCATTG GGAGAGTGCT GAAGGTTTTT GGAGACGGGA ACGTGGGTGT GCTGGTCAGT 
GGGAAGCTGT GGACCTTCAG CCCCTCCTGC CTGGTGGCCT ACCGGCCTGA GGAAGATGCC 
AACCTGGACG TAGCTGGGCG AGCCCGGGAG AACAAAAGCT CCCTGAGTGT TGTCCTGGAC 
AAGCTTCGAG CCCAGAAGAG TGACCTGGAG CACCCAGGGA GGTTGGTGGT GGAGGTGGCT 
CTGGGCAACA TGGCCCGGGC TCTGGACCTG CTGCGGCGGC ACCCAGAACA GGTGGACACC 
AAGAATCAGG GCAGGACTGC CCTGCAGATG GCTGCCTACC TAGGTCAGGT GGAGCTGGTG 
CGGCTGCTGC TGCAGGCTCA GGCGGGCGTT GACCTGCCAG ATGACGAGGG CAACACGGCA 
CTGCACTACG CCGCCCTGGG GAACCAGCCT GAGGCTGCCC GGGTGCTCTT GAGCTCGGGT 
TGTGGGGCCA ATGCTCTGAA CAGCACTCGG AGCTCAGCAC TGCATGTGGC TGTGCAAAGG 
GGCTTCCTGG AGGTGGTGAA GGTCCTCTGT GAGCGTGGCT GTGATGTCAA CCTGCCTGAT 
GCCCATGCTG ACACACCCCT GCACTGCGCC ATCTCGGCGG GCACTGGTGC CAGCGGCATT 
GTGGAGGTCC TCACCGAGGT GCCAGGCATC GACGTCACTG CCACCAACAG CCAGGGCTTC 
ACCCTGCTGC ACCATGCATC CCTCAAAGGC CACACACTAG CTGTCAGGAG GATTCTGGCG 
CGGGCCCGGC AGCTGGTGGA CGCCAAGAAG GAGGATGGGT TCACGGCACT GCACCTGGCC 
GCCTTCAACA ACCACGGGGA GGTGGCACAG GTTCTCATCC AAGAGGGCCA CTGTGATGTG 
AACGCTCGCA ACCGTAAGCT GCAGTCCCCG CTGCACCTGG CTGTGCAGCA GGCCCACGTG 
GGGCTGGTGC CACTGCTGGT GGACGCCGGC TGCAGCGTCA ACGCCGAGGA CGAGGAGGGG 
GACACGGCTC TGCACGTGGC CCTGCAGCGC CACCAGCTGC TGCCTCTGGT AGCTGATGGG 
GGTGCAGGGG ACCCGGGTCC CCTGCAGTTG CTGTCCAGGC TACAGGCCTC GGGCCTTCCA 
GGCAGCGCGG AGCTGACGGC GGGCGCAGCG ATAGCCTACT TCTTAGCGCT GGAGGGCGCT 
GACCTGAGCT ACGCCAACCA CCGCGGCCGG AGCCCCCTGG ACCTGGCTGC CGAGGGTCGC 
CTGCTCAAAG CCCTGCAGGG CTGTGCTCAG CGCTTCCGAC CCCTGTCCCC ACGCAGGGAG 
CGGCAGGCTT GCGGCGGTGG CAGCGGGGGC TCGGCCCAGG GCCCAAGACT GGTGCTCAGT 
GCTCCGAACA CTGTGACCAA CCTGCATGTG GCCACGCCGA CGGGGCCTGA GGCCGCTGAG 
TGCCTAGTGT GCTCAGAGCT GGCGCTGCTG GTGCTGTTCT CGCCCTGCCA GCATCGCACA 
GTGTGCGAGG AGTGCGCGCG CAGGATGAAG AAATGCATCA GGTGCCAGGT GGTCATCAGC 
AAGAAGCTGC GCCCAGGTGG GGCCCGGGGC CCGGCGCCCG CCCCGCCCCG CTGCCCCCAG 
ACGGCACGGA GGTGGTCAGC GCGGCCCCCG CGCCCGGCCC ACCACGGCAG TTGGTGGAGG 
AGCTGCAGAG CCGCTACCGG CAGATGGAGG AGCGCATCAC CTGCCCGATT TGCATCGACA 
GCCACATCCG CCTCGTGTTC CAGTGCGGCC ATGGCGCGTG CGCGCCCTGT GGCGCGGCGC 
TCAGCGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGATCG CATCCAGATC TTCGTGTAGC 
CGGCGCGCCC CCCCCCCCCG CCCCTTTCCG GGGCCATGCC CCGCAGCCGC CCTCGCAAGC 
CCTTTCTCTG TGTTTTATAA AAAGATTGCT GGACTTTGTG CCTGGTTGCC TGTCTGGGGC 
GGGGCCGTGC AGGTCCCCCA ACCGGGGACC GTTCTGACTG CACCTCTTAC ACCGTTGGCC 
CAGCGCTGGG TTCTGCGCTT TGGCCAAAGG CCTTGCAGGG CTTCTTTCCG ATCCGTATTC 
TGCCCTTCTT CCCAGGACTC ACGGGTCCAG AGTGCTGCCC GCACCCTGGC CGGAAAGAAC 
GGGGCCCGGG CTTGTGGTGT CGTCCGGGGG TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011993875.1
CDS	217..3072
Translation	MLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYREHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHETRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEWTDDMAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGRLVVEVALGNMARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGVDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGGAGDPGPLQLLSRLQASGLPGSAELTAGAAIAYFLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRPLSPRRERQACGGGSGGSAQGPRLVLSAPNTVTNLHVATPTGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPGGARGPAPAPPRCPQTARRWSARPPRPAHHGSWWRSCRAATGRWRSASPARFASTATSASCSSAAMARARPVARRSAPAPSAASPSAIASRSSCSRRAPPPRPFPGPCPAAALASPFSVFYKKIAGLCAWLPVWGGAVQVPQPGTVLTAPLTPLAQRWVLRFGQRPCRASFRSVFCPSSQDSRVQSAARTLAGKNGARACGVVRGY

Target ORF information:

RefSeq Version	XM_012138485.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012138485.2

ORF Insert Sequence:

ATGCTGAGTC CCCGCCAGGG CCTCCCAAGG ATCCCGTTAA GGGGCATCTT CCAGGGGGCG 
AAGGTGGTTC GGGGCCCCGA CTGGGAGTGG GGCTCACAGG ATGGAGGAGA AGGGAAGCCA 
GGCCGAGTAG TGGACATCCG TGGCTGGGAC GTGGAGACAG GCCGGAGTGT GGCCAGTGTG 
ACATGGGCTG ATGGCACCAC CAACGTGTAT CGTGTGGGCC ACAAGGGCAA GGTGGACCTC 
AAGTGTGTGG GCGAGGCAGC TGGCGGCTTC TACTACAGGG AGCACCTCCC AAGGCTTGGC 
AAGCCTGCAG AGCTGCAGCG CAGGGTCAGT GCTGATGGCC AGCCCTTCCA GCGTGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACAGACGTG CTGAGGGAGA TGCAGGAAGG CCACGGCGGG 
TGGAACCCCC GGATGGCCAA GTTTATCGGA CAGACGGGCA CCGTGCACCG CATCACAGAC 
CGTGGGGACG TGCGTGTGCA GTTCAGCCAC GAGACCCGCT GGACCTTCCA TCCTGGGGCT 
CTCACCAAGC AAAACTCCTG CTTGGTGGGT GAGGTGGTGC GGGTTATCGA CGACCTTGAC 
ACGGTGAAGC GGCTGCAGGC TGGCCATGGG GAGTGGACCG ATGACATGGC CCCTGCTCTG 
GGCCGCATTG GGAGAGTGCT GAAGGTTTTT GGAGACGGGA ACGTGGGTGT GCTGGTCAGT 
GGGAAGCTGT GGACCTTCAG CCCCTCCTGC CTGGTGGCCT ACCGGCCTGA GGAAGATGCC 
AACCTGGACG TAGCTGGGCG AGCCCGGGAG AACAAAAGCT CCCTGAGTGT TGTCCTGGAC 
AAGCTTCGAG CCCAGAAGAG TGACCTGGAG CACCCAGGGA GGTTGGTGGT GGAGGTGGCT 
CTGGGCAACA TGGCCCGGGC TCTGGACCTG CTGCGGCGGC ACCCAGAACA GGTGGACACC 
AAGAATCAGG GCAGGACTGC CCTGCAGATG GCTGCCTACC TAGGTCAGGT GGAGCTGGTG 
CGGCTGCTGC TGCAGGCTCA GGCGGGCGTT GACCTGCCAG ATGACGAGGG CAACACGGCA 
CTGCACTACG CCGCCCTGGG GAACCAGCCT GAGGCTGCCC GGGTGCTCTT GAGCTCGGGT 
TGTGGGGCCA ATGCTCTGAA CAGCACTCGG AGCTCAGCAC TGCATGTGGC TGTGCAAAGG 
GGCTTCCTGG AGGTGGTGAA GGTCCTCTGT GAGCGTGGCT GTGATGTCAA CCTGCCTGAT 
GCCCATGCTG ACACACCCCT GCACTGCGCC ATCTCGGCGG GCACTGGTGC CAGCGGCATT 
GTGGAGGTCC TCACCGAGGT GCCAGGCATC GACGTCACTG CCACCAACAG CCAGGGCTTC 
ACCCTGCTGC ACCATGCATC CCTCAAAGGC CACACACTAG CTGTCAGGAG GATTCTGGCG 
CGGGCCCGGC AGCTGGTGGA CGCCAAGAAG GAGGATGGGT TCACGGCACT GCACCTGGCC 
GCCTTCAACA ACCACGGGGA GGTGGCACAG GTTCTCATCC AAGAGGGCCA CTGTGATGTG 
AACGCTCGCA ACCGTAAGCT GCAGTCCCCG CTGCACCTGG CTGTGCAGCA GGCCCACGTG 
GGGCTGGTGC CACTGCTGGT GGACGCCGGC TGCAGCGTCA ACGCCGAGGA CGAGGAGGGG 
GACACGGCTC TGCACGTGGC CCTGCAGCGC CACCAGCTGC TGCCTCTGGT AGCTGATGGG 
GGTGCAGGGG ACCCGGGTCC CCTGCAGTTG CTGTCCAGGC TACAGGCCTC GGGCCTTCCA 
GGCAGCGCGG AGCTGACGGC GGGCGCAGCG ATAGCCTACT TCTTAGCGCT GGAGGGCGCT 
GACCTGAGCT ACGCCAACCA CCGCGGCCGG AGCCCCCTGG ACCTGGCTGC CGAGGGTCGC 
CTGCTCAAAG CCCTGCAGGG CTGTGCTCAG CGCTTCCGAC CCCTGTCCCC ACGCAGGGAG 
CGGCAGGCTT GCGGCGGTGG CAGCGGGGGC TCGGCCCAGG GCCCAAGACT GGTGCTCAGT 
GCTCCGAACA CTGTGACCAA CCTGCATGTG GCCACGCCGA CGGGGCCTGA GGCCGCTGAG 
TGCCTAGTGT GCTCAGAGCT GGCGCTGCTG GTGCTGTTCT CGCCCTGCCA GCATCGCACA 
GTGTGCGAGG AGTGCGCGCG CAGGATGAAG AAATGCATCA GGTGCCAGGT GGTCATCAGC 
AAGAAGCTGC GCCCAGGTGG GGCCCGGGGC CCGGCGCCCG CCCCGCCCCG CTGCCCCCAG 
ACGGCACGGA GGTGGTCAGC GCGGCCCCCG CGCCCGGCCC ACCACGGCAG TTGGTGGAGG 
AGCTGCAGAG CCGCTACCGG CAGATGGAGG AGCGCATCAC CTGCCCGATT TGCATCGACA 
GCCACATCCG CCTCGTGTTC CAGTGCGGCC ATGGCGCGTG CGCGCCCTGT GGCGCGGCGC 
TCAGCGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGATCG CATCCAGATC TTCGTGTAGC 
CGGCGCGCCC CCCCCCCCCG CCCCTTTCCG GGGCCATGCC CCGCAGCCGC CCTCGCAAGC 
CCTTTCTCTG TGTTTTATAA AAAGATTGCT GGACTTTGTG CCTGGTTGCC TGTCTGGGGC 
GGGGCCGTGC AGGTCCCCCA ACCGGGGACC GTTCTGACTG CACCTCTTAC ACCGTTGGCC 
CAGCGCTGGG TTCTGCGCTT TGGCCAAAGG CCTTGCAGGG CTTCTTTCCG ATCCGTATTC 
TGCCCTTCTT CCCAGGACTC ACGGGTCCAG AGTGCTGCCC GCACCCTGGC CGGAAAGAAC 
GGGGCCCGGG CTTGTGGTGT CGTCCGGGGG TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MIB2
Entrez Gene ID	101102748
Full Name	mindbomb E3 ubiquitin protein ligase 2
Gene Type	protein-coding
Organism	Ovis aries(sheep)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MIB2 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

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