crim1 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	crim1
Entrez Gene ID	101173025
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020713843.1	XP_020569502.1	cysteine-rich motor neuron 1 protein
XM_023951833.1	XP_023807601.1	cysteine-rich motor neuron 1 protein

Pathways from BioSystems
Gene Ontology

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OJa113897	XM_023951833.1 Latest version!	Oryzias latipes cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OJa75947	XM_020713843.1 Latest version!	Oryzias latipes cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_023951833.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023807601.1 Oryzias latipes cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Price & Availability↑

CloneID	OJa113897
Clone ID Related Accession (Same CDS sequence)	XM_023951833.1
Accession Version	XM_023951833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-14
Organism	Oryzias latipes(Japanese medaka)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAACA AATTCTCAAC TGTGTTGTTG AATGCGCTCT TTCTTGTGCG GGCGCTCTCG 
GCTCACGAGT GCACGCTCTG TAACCTCCGG ACCTGTCCCA TTAAACCCCC GCATGGCTGC 
GAGGGTGGCC GGAGCGTGGC TAAGGATCCA TGCGGATGCT GTGATCAGTG CGCGCGGCTG 
GAGTGGGAGC CGTGCGGCGG TCCGGACTGG ACGCTCGGTT ACTGCGCTCT GGGTCTGACC 
TGCGCCTCTC TTAACGGCAC GCGAGCCGCC ACCATACCTG AGACTGGAGT TTGTAAAGTT 
CTTCCACAGC ATCCAGATGC AGGTCTCGAG GATGAGCGCT GCCCCCTGAT AACAGGCTGC 
GACAAAGCTG GAAATCAGTG CTTATGCGAT GCCCGCCACT CCTGCTTGAG CTCCTTTACA 
TACCCAGACC AAGAAGCTTG CATGAAATCC ATCAAGTCAG ATGGTCGGAG GCACGAGCAC 
CGGGAGAGAC ACAGGGAAAA GTACGTGGGA CCTTCCAACC CGGCGTGCAT GTTCTCTGGG 
TGTAACCTGA CCGCTGAGGG CTGCGTGTGC GAGTCTCAGA GCTGCCACCA CCACTTTGCC 
TACATCAACC GAAGCCAATG CCAGGAAGCA GCAGATCAGT TGAGGTGTGC TGGGGTTAAA 
TGCCCAACTT TGCCAGTGCC TTCCTGCCCC GAAGACTCAG TCCTGACTAA GAGTTACACG 
CCCCCTGCTG GCTGCTGCCC ATCCGTAACA CCCCAGTGTT CTTGCAACTT CCGGGCCTGC 
CAGAAACTCC AGTGCCCAAG CGGAGAGCAC CGGGTCCTCC TCAGTCAAGC CAGCGGTCAG 
CCAGGAAACT GCTGCGATGT CTTTGAGTGT CGTAAAGTCT CTCCCAAATG TGTCCATAAT 
GGGAAGGAGC TCTCTGATGG AGAGGTGTAC CGCATGGACT CCTGCTGGCT GTGCCAGTGC 
AGAGGAGGAA TCTCCTTCTG CTCCAAGGCA GAGTGTGCTG AGCTGGACTG TGAAAACTTC 
TACGTTCCTG AGGGCGAGTG CTGCCCTGTC TGCATAGATG TGGAGTTGTT GTCTGTGGAC 
AGCACCAAGG CCAGCTGCTG GGTGAACAAC AAGCTGCGAG CGCATGAGGA GCAGTGGAAA 
GAGGACGACT GCACCTTCTG CCAGTGTGTG GATGGAGATC CACACTGCAC GGCCATGGCC 
TGCAAACAGA GCTGCCAAAA TCCTGTGAAG ATCCCAGGAG AGTGCTGCCC ATTCTGCGAA 
GAGCCAACTT ATGAAACCGT GAGCCCGATT CTCTGCCCTC CCTTGCAAAA CTGCTCCCTG 
TCAGTGAACG ACTGTCCTTA TGGCTTCTAT CAGGATGTAA ACGGGTGTCT CCTTTGCCAG 
TGTCTCAACA ATGACTCCTG CCCAGACCTG GGAAGACACT GCTCTCTCCA GTGCCCACTG 
GGATTTGAGA AGGATGACTT TGGCTGTGAG GTGTGTGATT GCGTCACTCC GATGCCCAAG 
TGCCGACCTC TGACCTGCAC AAAGACCTGC CCATATGGAT ATGTGAGGAA CAAACACGGC 
TGTGAGATGT GTCGCTGTGT CAAGTGTCCG CCCTTTACCT GTGACAAGCA CTGCAATAAC 
GGCTACCGGC AGAACAGAAA GGGCTGCTCT GTGTGCATGT GCAAAGAAAG CATCCCCAGC 
CCCACACTCC CAGCTCCAAC GCCCAGCTAT TGTCTCACCT CCAATGGACA CCGCTATGAA 
GAAGGTGACA GCTGGCATGA TGGCTGCCGT GACTGTTATT GCCACTCTGG GAGGGAGATG 
TGCGTGCTCA TTTCCTGCCC TATGCCCAGC TGCTCACATC CTGTTGTCAA GCCGGACCAG 
TGCTGTCCTT CCTGTGAAGA CGAGTCAGGC AGTGGTCAGC CAGAAGGAAT GGATGTCATG 
GTCTGTAGAG CACCTGGAGG GGAGTTTTAT GTGGAAGGAG AGACCTGGAA CCTGGACGAG 
TGCACTCGCT GCACCTGCAG GAAGGGACGA GTCCTGTGCG ACACCGAGGT GTGTCCTCCA 
GCTCTCTGCC AGACACCGAT CAGAAACAAG GATACTTGCT GCCACGTTTG TCCAGAGGAG 
ACACTGGGCC CCTTGCTGCC CGTGAACAGA AGTCAGCAGC AGTACTGCCT AACTGTTGAA 
GGAGAGGTGC TGCTTGCTGG TGACTCCTGG AGGACTAACG CCTGCACCAG CTGCACTTGC 
AACAACGGGA CCATTCAGTG TTTCTCCCAG CGCTGTCCAG CCGCCAACTG CAGGGTACCG 
GTTTTACGCA AGGGCCAGTG CTGCCCGCAC TGTCTCGAGG TGACCACATC AGTGCCAGTG 
AAAGTGACCG TCGATACCAT CACCACTTCA CTGGAGATTG TATTTGAGAT GACCACAGTC 
ACTGTTCCAC CCATCACTGC TGACACAGAT GGGCTAGAGT TTGGTGATAA TTACCCCAGC 
CAAGCTGAGA TGGCCCTTGT GTACCAATCA GCGGCCTGGA TTCTGGCAGG CCTCCTCTTG 
GCCATCATCA TCTTCCTCTT TGTTGCGTTT TTCATCAACA AGAAAAAGAA ATGGGTGCAG 
ATGTCCTGCT ATAGTGCACC AAAGAAGACG GTGATCCTGA AAAAGCACAT AAACAAGAAC 
TCTGTGGTCT ACATGGAGCC CTCCAAGGAG AACAAGTTTC AGAATGTGAA GAACGACTGT 
GGCGTCATTC ACTTCTCTCC AGACATGAGT TCCCCTTGCA AAGACAGGAT GACCATCCCC 
AGGGCCAAAC TAGCCAATGA CTGGACGTCA CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023807601.1
CDS	304..3099
Translation	MFNKFSTVLLNALFLVRALSAHECTLCNLRTCPIKPPHGCEGGRSVAKDPCGCCDQCARLEWEPCGGPDWTLGYCALGLTCASLNGTRAATIPETGVCKVLPQHPDAGLEDERCPLITGCDKAGNQCLCDARHSCLSSFTYPDQEACMKSIKSDGRRHEHRERHREKYVGPSNPACMFSGCNLTAEGCVCESQSCHHHFAYINRSQCQEAADQLRCAGVKCPTLPVPSCPEDSVLTKSYTPPAGCCPSVTPQCSCNFRACQKLQCPSGEHRVLLSQASGQPGNCCDVFECRKVSPKCVHNGKELSDGEVYRMDSCWLCQCRGGISFCSKAECAELDCENFYVPEGECCPVCIDVELLSVDSTKASCWVNNKLRAHEEQWKEDDCTFCQCVDGDPHCTAMACKQSCQNPVKIPGECCPFCEEPTYETVSPILCPPLQNCSLSVNDCPYGFYQDVNGCLLCQCLNNDSCPDLGRHCSLQCPLGFEKDDFGCEVCDCVTPMPKCRPLTCTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCNNGYRQNRKGCSVCMCKESIPSPTLPAPTPSYCLTSNGHRYEEGDSWHDGCRDCYCHSGREMCVLISCPMPSCSHPVVKPDQCCPSCEDESGSGQPEGMDVMVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQTPIRNKDTCCHVCPEETLGPLLPVNRSQQQYCLTVEGEVLLAGDSWRTNACTSCTCNNGTIQCFSQRCPAANCRVPVLRKGQCCPHCLEVTTSVPVKVTVDTITTSLEIVFEMTTVTVPPITADTDGLEFGDNYPSQAEMALVYQSAAWILAGLLLAIIIFLFVAFFINKKKKWVQMSCYSAPKKTVILKKHINKNSVVYMEPSKENKFQNVKNDCGVIHFSPDMSSPCKDRMTIPRAKLANDWTSR

Target ORF information:

RefSeq Version	XM_023951833.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023951833.1

ORF Insert Sequence:

ATGTTTAACA AATTCTCAAC TGTGTTGTTG AATGCGCTCT TTCTTGTGCG GGCGCTCTCG 
GCTCACGAGT GCACGCTCTG TAACCTCCGG ACCTGTCCCA TTAAACCCCC GCATGGCTGC 
GAGGGTGGCC GGAGCGTGGC TAAGGATCCA TGCGGATGCT GTGATCAGTG CGCGCGGCTG 
GAGTGGGAGC CGTGCGGCGG TCCGGACTGG ACGCTCGGTT ACTGCGCTCT GGGTCTGACC 
TGCGCCTCTC TTAACGGCAC GCGAGCCGCC ACCATACCTG AGACTGGAGT TTGTAAAGTT 
CTTCCACAGC ATCCAGATGC AGGTCTCGAG GATGAGCGCT GCCCCCTGAT AACAGGCTGC 
GACAAAGCTG GAAATCAGTG CTTATGCGAT GCCCGCCACT CCTGCTTGAG CTCCTTTACA 
TACCCAGACC AAGAAGCTTG CATGAAATCC ATCAAGTCAG ATGGTCGGAG GCACGAGCAC 
CGGGAGAGAC ACAGGGAAAA GTACGTGGGA CCTTCCAACC CGGCGTGCAT GTTCTCTGGG 
TGTAACCTGA CCGCTGAGGG CTGCGTGTGC GAGTCTCAGA GCTGCCACCA CCACTTTGCC 
TACATCAACC GAAGCCAATG CCAGGAAGCA GCAGATCAGT TGAGGTGTGC TGGGGTTAAA 
TGCCCAACTT TGCCAGTGCC TTCCTGCCCC GAAGACTCAG TCCTGACTAA GAGTTACACG 
CCCCCTGCTG GCTGCTGCCC ATCCGTAACA CCCCAGTGTT CTTGCAACTT CCGGGCCTGC 
CAGAAACTCC AGTGCCCAAG CGGAGAGCAC CGGGTCCTCC TCAGTCAAGC CAGCGGTCAG 
CCAGGAAACT GCTGCGATGT CTTTGAGTGT CGTAAAGTCT CTCCCAAATG TGTCCATAAT 
GGGAAGGAGC TCTCTGATGG AGAGGTGTAC CGCATGGACT CCTGCTGGCT GTGCCAGTGC 
AGAGGAGGAA TCTCCTTCTG CTCCAAGGCA GAGTGTGCTG AGCTGGACTG TGAAAACTTC 
TACGTTCCTG AGGGCGAGTG CTGCCCTGTC TGCATAGATG TGGAGTTGTT GTCTGTGGAC 
AGCACCAAGG CCAGCTGCTG GGTGAACAAC AAGCTGCGAG CGCATGAGGA GCAGTGGAAA 
GAGGACGACT GCACCTTCTG CCAGTGTGTG GATGGAGATC CACACTGCAC GGCCATGGCC 
TGCAAACAGA GCTGCCAAAA TCCTGTGAAG ATCCCAGGAG AGTGCTGCCC ATTCTGCGAA 
GAGCCAACTT ATGAAACCGT GAGCCCGATT CTCTGCCCTC CCTTGCAAAA CTGCTCCCTG 
TCAGTGAACG ACTGTCCTTA TGGCTTCTAT CAGGATGTAA ACGGGTGTCT CCTTTGCCAG 
TGTCTCAACA ATGACTCCTG CCCAGACCTG GGAAGACACT GCTCTCTCCA GTGCCCACTG 
GGATTTGAGA AGGATGACTT TGGCTGTGAG GTGTGTGATT GCGTCACTCC GATGCCCAAG 
TGCCGACCTC TGACCTGCAC AAAGACCTGC CCATATGGAT ATGTGAGGAA CAAACACGGC 
TGTGAGATGT GTCGCTGTGT CAAGTGTCCG CCCTTTACCT GTGACAAGCA CTGCAATAAC 
GGCTACCGGC AGAACAGAAA GGGCTGCTCT GTGTGCATGT GCAAAGAAAG CATCCCCAGC 
CCCACACTCC CAGCTCCAAC GCCCAGCTAT TGTCTCACCT CCAATGGACA CCGCTATGAA 
GAAGGTGACA GCTGGCATGA TGGCTGCCGT GACTGTTATT GCCACTCTGG GAGGGAGATG 
TGCGTGCTCA TTTCCTGCCC TATGCCCAGC TGCTCACATC CTGTTGTCAA GCCGGACCAG 
TGCTGTCCTT CCTGTGAAGA CGAGTCAGGC AGTGGTCAGC CAGAAGGAAT GGATGTCATG 
GTCTGTAGAG CACCTGGAGG GGAGTTTTAT GTGGAAGGAG AGACCTGGAA CCTGGACGAG 
TGCACTCGCT GCACCTGCAG GAAGGGACGA GTCCTGTGCG ACACCGAGGT GTGTCCTCCA 
GCTCTCTGCC AGACACCGAT CAGAAACAAG GATACTTGCT GCCACGTTTG TCCAGAGGAG 
ACACTGGGCC CCTTGCTGCC CGTGAACAGA AGTCAGCAGC AGTACTGCCT AACTGTTGAA 
GGAGAGGTGC TGCTTGCTGG TGACTCCTGG AGGACTAACG CCTGCACCAG CTGCACTTGC 
AACAACGGGA CCATTCAGTG TTTCTCCCAG CGCTGTCCAG CCGCCAACTG CAGGGTACCG 
GTTTTACGCA AGGGCCAGTG CTGCCCGCAC TGTCTCGAGG TGACCACATC AGTGCCAGTG 
AAAGTGACCG TCGATACCAT CACCACTTCA CTGGAGATTG TATTTGAGAT GACCACAGTC 
ACTGTTCCAC CCATCACTGC TGACACAGAT GGGCTAGAGT TTGGTGATAA TTACCCCAGC 
CAAGCTGAGA TGGCCCTTGT GTACCAATCA GCGGCCTGGA TTCTGGCAGG CCTCCTCTTG 
GCCATCATCA TCTTCCTCTT TGTTGCGTTT TTCATCAACA AGAAAAAGAA ATGGGTGCAG 
ATGTCCTGCT ATAGTGCACC AAAGAAGACG GTGATCCTGA AAAAGCACAT AAACAAGAAC 
TCTGTGGTCT ACATGGAGCC CTCCAAGGAG AACAAGTTTC AGAATGTGAA GAACGACTGT 
GGCGTCATTC ACTTCTCTCC AGACATGAGT TCCCCTTGCA AAGACAGGAT GACCATCCCC 
AGGGCCAAAC TAGCCAATGA CTGGACGTCA CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crim1 ( XM_020713843.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020569502.1 Oryzias latipes cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Price & Availability↑

CloneID	OJa75947
Clone ID Related Accession (Same CDS sequence)	XM_020713843.1
Accession Version	XM_020713843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-06
Organism	Oryzias latipes(Japanese medaka)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGTTTAACA AATTCTCAAC TGTGTTGTTG AATGCGCTCT TTCTTGTGCG GGCGCTCTCG 
GCTCACGAGT GCACGCTCTG TAACCTCCGG ACCTGTCCCA TTAAACCCCC GCATGGCTGC 
GAGGGTGGCC GGAGCGTGGC TAAGGATCCA TGCGGATGCT GTGATCAGTG CGCGCGGCTG 
GAGTGGGAGC CGTGCGGCGG TCCGGACTGG ACGCTCGGTT ACTGCGCTCT GGGTCTGACC 
TGCGCCTCTC TTAACGGCAC GCGAGCCGCC ACCATACCTG AGACTGGAGT TTGTAAAGTT 
CTTCCACAGC ATCCAGATGC AGGTCTCGAG GATGAGCGCT GCCCCCTGAT AACAGGCTGC 
GACAAAGCTG GAAATCAGTG CTTATGCGAT GCCCGCCACT CCTGCTTGAG CTCCTTTACA 
TACCCAGACC AAGAAGCTTG CATGAAATCC ATCAAGTCAG ATGGTCGGAG GCACGAGCAC 
CGGGAGAGAC ACAGGGAAAA GTACGTGGGA CCTTCCAACC CGGCGTGCAT GTTCTCTGGG 
TGTAACCTGA CCGCTGAGGG CTGCGTGTGC GAGTCTCAGA GCTGCCACCA CCACTTTGCC 
TACATCAACC GAAGCCAATG CCAGGAAGCA GCAGATCAGT TGAGGTGTGC TGGGGTTAAA 
TGCCCAACTT TGCCAGTGCC TTCCTGCCCC GAAGACTCAG TCCTGACTAA GAGTTACACG 
CCCCCTGCTG GCTGCTGCCC ATCCGTAACA CCCCAGTGTT CTTGCAACTT CCGGGCCTGC 
CAGAAACTCC AGTGCCCAAG CGGAGAGCAC CGGGTCCTCC TCAGTCAAGC CAGCGGTCAG 
CCAGGAAACT GCTGCGATGT CTTTGAGTGT CGTAAAGTCT CTCCCAAATG TGTCCATAAT 
GGGAAGGAGC TCTCTGATGG AGAGGTGTAC CGCATGGACT CCTGCTGGCT GTGCCAGTGC 
AGAGGAGGAA TCTCCTTCTG CTCCAAGGCA GAGTGTGCTG AGCTGGACTG TGAAAACTTC 
TACGTTCCTG AGGGCGAGTG CTGCCCTGTC TGCATAGATG TGGAGTTGTT GTCTGTGGAC 
AGCACCAAGG CCAGCTGCTG GGTGAACAAC AAGCTGCGAG CGCATGAGGA GCAGTGGAAA 
GAGGACGACT GCACCTTCTG CCAGTGTGTG GATGGAGATC CACACTGCAC GGCCATGGCC 
TGCAAACAGA GCTGCCAAAA TCCTGTGAAG ATCCCAGGAG AGTGCTGCCC ATTCTGCGAA 
GAGCCAACTT ATGAAACCGT GAGCCCGATT CTCTGCCCTC CCTTGCAAAA CTGCTCCCTG 
TCAGTGAACG ACTGTCCTTA TGGCTTCTAT CAGGATGTAA ACGGGTGTCT CCTTTGCCAG 
TGTCTCAACA ATGACTCCTG CCCAGACCTG GGAAGACACT GCTCTCTCCA GTGCCCACTG 
GGATTTGAGA AGGATGACTT TGGCTGTGAG GTGTGTGATT GCGTCACTCC GATGCCCAAG 
TGCCGACCTC TGACCTGCAC AAAGACCTGC CCATATGGAT ATGTGAGGAA CAAACACGGC 
TGTGAGATGT GTCGCTGTGT CAAGTGTCCG CCCTTTACCT GTGACAAGCA CTGCAATAAC 
GGCTACCGGC AGAACAGAAA GGGCTGCTCT GTGTGCATGT GCAAAGAAAG CATCCCCAGC 
CCCACACTCC CAGCTCCAAC GCCCAGCTAT TGTCTCACCT CCAATGGACA CCGCTATGAA 
GAAGGTGACA GCTGGCATGA TGGCTGCCGT GACTGTTATT GCCACTCTGG GAGGGAGATG 
TGCGTGCTCA TTTCCTGCCC TATGCCCAGC TGCTCACATC CTGTTGTCAA GCCGGACCAG 
TGCTGTCCTT CCTGTGAAGG TAGCCATGTT TTTTCCCTGG TTTTCTTTTT CATAGACATA 
CAAATATACC TTGAATTTTT ATTTTATCTC CAACTCATAA AGATTATTTG TGAAGGGGTG 
ACTAAGATGT CATCTGGAAC ATTTAGGAGT CCAGGAATGT CTACAACCTA CGGAAAGGAG 
ACACTGGGCC CCTTGCTGCC CGTGAACAGA AGTCAGCAGC AGTACTGCCT AACTGTTGAA 
GGAGAGGTGC TGCTTGCTGG TGACTCCTGG AGGACTAACG CCTGCACCAG CTGCACTTGC 
AACAACGGGA CCATTCAGTG TTTCTCCCAG CGCTGTCCAG CCGCCAACTG CAGGGTACCG 
GTTTTACGCA AGGGCCAGTG CTGCCCGCAC TGTCTCGAGG TGACCACATC AGTGCCAGTG 
AAAGTGACCG TCGATACCAT CACCACTTCA CTGGAGATTG TATTTGAGAT GACCACAGTC 
ACTGTTCCAC CCATCACTGC TGACACAGAT GGGCTAGAGT TTGGTGATAA TTACCCCAGC 
CAAGCTGAGA TGGCCCTTGT GTACCAATCA GCGGCCTGGA TTCTGGCAGG CCTCCTCTTG 
GCCATCATCA TCTTCCTCTT TGTTGCGTTT TTCATCAACA AGAAAAAGAA ATGGGTGCAG 
ATGTCCTGCT ATAGTGCACC AAAGAAGACG GTGATCCTGA AAAAGCACAT AAACAAGAAC 
TCTGTGGTCT ACATGGAGCC CTCCAAGGAG AACAAGTTTC AGAATGTGAA GAACGACTGT 
GGCGTCATTC ACTTCTCTCC AGACATGAGT TCCCCTTGCA AAGACAGGAT GACCATCCCC 
AGGGCCAAAC TAGCCAATGA CTGGACGTCA CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020569502.1
CDS	308..3043
Translation	MFNKFSTVLLNALFLVRALSAHECTLCNLRTCPIKPPHGCEGGRSVAKDPCGCCDQCARLEWEPCGGPDWTLGYCALGLTCASLNGTRAATIPETGVCKVLPQHPDAGLEDERCPLITGCDKAGNQCLCDARHSCLSSFTYPDQEACMKSIKSDGRRHEHRERHREKYVGPSNPACMFSGCNLTAEGCVCESQSCHHHFAYINRSQCQEAADQLRCAGVKCPTLPVPSCPEDSVLTKSYTPPAGCCPSVTPQCSCNFRACQKLQCPSGEHRVLLSQASGQPGNCCDVFECRKVSPKCVHNGKELSDGEVYRMDSCWLCQCRGGISFCSKAECAELDCENFYVPEGECCPVCIDVELLSVDSTKASCWVNNKLRAHEEQWKEDDCTFCQCVDGDPHCTAMACKQSCQNPVKIPGECCPFCEEPTYETVSPILCPPLQNCSLSVNDCPYGFYQDVNGCLLCQCLNNDSCPDLGRHCSLQCPLGFEKDDFGCEVCDCVTPMPKCRPLTCTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCNNGYRQNRKGCSVCMCKESIPSPTLPAPTPSYCLTSNGHRYEEGDSWHDGCRDCYCHSGREMCVLISCPMPSCSHPVVKPDQCCPSCEGSHVFSLVFFFIDIQIYLEFLFYLQLIKIICEGVTKMSSGTFRSPGMSTTYGKETLGPLLPVNRSQQQYCLTVEGEVLLAGDSWRTNACTSCTCNNGTIQCFSQRCPAANCRVPVLRKGQCCPHCLEVTTSVPVKVTVDTITTSLEIVFEMTTVTVPPITADTDGLEFGDNYPSQAEMALVYQSAAWILAGLLLAIIIFLFVAFFINKKKKWVQMSCYSAPKKTVILKKHINKNSVVYMEPSKENKFQNVKNDCGVIHFSPDMSSPCKDRMTIPRAKLANDWTSR

Target ORF information:

RefSeq Version	XM_020713843.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020713843.1

ORF Insert Sequence:

ATGTTTAACA AATTCTCAAC TGTGTTGTTG AATGCGCTCT TTCTTGTGCG GGCGCTCTCG 
GCTCACGAGT GCACGCTCTG TAACCTCCGG ACCTGTCCCA TTAAACCCCC GCATGGCTGC 
GAGGGTGGCC GGAGCGTGGC TAAGGATCCA TGCGGATGCT GTGATCAGTG CGCGCGGCTG 
GAGTGGGAGC CGTGCGGCGG TCCGGACTGG ACGCTCGGTT ACTGCGCTCT GGGTCTGACC 
TGCGCCTCTC TTAACGGCAC GCGAGCCGCC ACCATACCTG AGACTGGAGT TTGTAAAGTT 
CTTCCACAGC ATCCAGATGC AGGTCTCGAG GATGAGCGCT GCCCCCTGAT AACAGGCTGC 
GACAAAGCTG GAAATCAGTG CTTATGCGAT GCCCGCCACT CCTGCTTGAG CTCCTTTACA 
TACCCAGACC AAGAAGCTTG CATGAAATCC ATCAAGTCAG ATGGTCGGAG GCACGAGCAC 
CGGGAGAGAC ACAGGGAAAA GTACGTGGGA CCTTCCAACC CGGCGTGCAT GTTCTCTGGG 
TGTAACCTGA CCGCTGAGGG CTGCGTGTGC GAGTCTCAGA GCTGCCACCA CCACTTTGCC 
TACATCAACC GAAGCCAATG CCAGGAAGCA GCAGATCAGT TGAGGTGTGC TGGGGTTAAA 
TGCCCAACTT TGCCAGTGCC TTCCTGCCCC GAAGACTCAG TCCTGACTAA GAGTTACACG 
CCCCCTGCTG GCTGCTGCCC ATCCGTAACA CCCCAGTGTT CTTGCAACTT CCGGGCCTGC 
CAGAAACTCC AGTGCCCAAG CGGAGAGCAC CGGGTCCTCC TCAGTCAAGC CAGCGGTCAG 
CCAGGAAACT GCTGCGATGT CTTTGAGTGT CGTAAAGTCT CTCCCAAATG TGTCCATAAT 
GGGAAGGAGC TCTCTGATGG AGAGGTGTAC CGCATGGACT CCTGCTGGCT GTGCCAGTGC 
AGAGGAGGAA TCTCCTTCTG CTCCAAGGCA GAGTGTGCTG AGCTGGACTG TGAAAACTTC 
TACGTTCCTG AGGGCGAGTG CTGCCCTGTC TGCATAGATG TGGAGTTGTT GTCTGTGGAC 
AGCACCAAGG CCAGCTGCTG GGTGAACAAC AAGCTGCGAG CGCATGAGGA GCAGTGGAAA 
GAGGACGACT GCACCTTCTG CCAGTGTGTG GATGGAGATC CACACTGCAC GGCCATGGCC 
TGCAAACAGA GCTGCCAAAA TCCTGTGAAG ATCCCAGGAG AGTGCTGCCC ATTCTGCGAA 
GAGCCAACTT ATGAAACCGT GAGCCCGATT CTCTGCCCTC CCTTGCAAAA CTGCTCCCTG 
TCAGTGAACG ACTGTCCTTA TGGCTTCTAT CAGGATGTAA ACGGGTGTCT CCTTTGCCAG 
TGTCTCAACA ATGACTCCTG CCCAGACCTG GGAAGACACT GCTCTCTCCA GTGCCCACTG 
GGATTTGAGA AGGATGACTT TGGCTGTGAG GTGTGTGATT GCGTCACTCC GATGCCCAAG 
TGCCGACCTC TGACCTGCAC AAAGACCTGC CCATATGGAT ATGTGAGGAA CAAACACGGC 
TGTGAGATGT GTCGCTGTGT CAAGTGTCCG CCCTTTACCT GTGACAAGCA CTGCAATAAC 
GGCTACCGGC AGAACAGAAA GGGCTGCTCT GTGTGCATGT GCAAAGAAAG CATCCCCAGC 
CCCACACTCC CAGCTCCAAC GCCCAGCTAT TGTCTCACCT CCAATGGACA CCGCTATGAA 
GAAGGTGACA GCTGGCATGA TGGCTGCCGT GACTGTTATT GCCACTCTGG GAGGGAGATG 
TGCGTGCTCA TTTCCTGCCC TATGCCCAGC TGCTCACATC CTGTTGTCAA GCCGGACCAG 
TGCTGTCCTT CCTGTGAAGG TAGCCATGTT TTTTCCCTGG TTTTCTTTTT CATAGACATA 
CAAATATACC TTGAATTTTT ATTTTATCTC CAACTCATAA AGATTATTTG TGAAGGGGTG 
ACTAAGATGT CATCTGGAAC ATTTAGGAGT CCAGGAATGT CTACAACCTA CGGAAAGGAG 
ACACTGGGCC CCTTGCTGCC CGTGAACAGA AGTCAGCAGC AGTACTGCCT AACTGTTGAA 
GGAGAGGTGC TGCTTGCTGG TGACTCCTGG AGGACTAACG CCTGCACCAG CTGCACTTGC 
AACAACGGGA CCATTCAGTG TTTCTCCCAG CGCTGTCCAG CCGCCAACTG CAGGGTACCG 
GTTTTACGCA AGGGCCAGTG CTGCCCGCAC TGTCTCGAGG TGACCACATC AGTGCCAGTG 
AAAGTGACCG TCGATACCAT CACCACTTCA CTGGAGATTG TATTTGAGAT GACCACAGTC 
ACTGTTCCAC CCATCACTGC TGACACAGAT GGGCTAGAGT TTGGTGATAA TTACCCCAGC 
CAAGCTGAGA TGGCCCTTGT GTACCAATCA GCGGCCTGGA TTCTGGCAGG CCTCCTCTTG 
GCCATCATCA TCTTCCTCTT TGTTGCGTTT TTCATCAACA AGAAAAAGAA ATGGGTGCAG 
ATGTCCTGCT ATAGTGCACC AAAGAAGACG GTGATCCTGA AAAAGCACAT AAACAAGAAC 
TCTGTGGTCT ACATGGAGCC CTCCAAGGAG AACAAGTTTC AGAATGTGAA GAACGACTGT 
GGCGTCATTC ACTTCTCTCC AGACATGAGT TCCCCTTGCA AAGACAGGAT GACCATCCCC 
AGGGCCAAAC TAGCCAATGA CTGGACGTCA CGCTAA