LOC4330371 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC4330371
Entrez Gene ID	4330371
Full Name	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1
Synonyms	OJ1743_B12.28,PIE1
Gene Type	protein-coding
Organism	Oryza sativa Japonica Group(Japanese rice)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015770118.2	XP_015625604.1	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X2
XM_015770121.1	XP_015625607.1	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X2
XM_015770119.1	XP_015625605.1	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X1
XM_015770121.2	XP_015625607.1	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X3
XM_015770118.1	XP_015625604.1	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X1
XM_026022820.1	XP_025878605.1	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X1
XM_026022821.1	XP_025878606.1	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	dom	NP_524833.2
Oryza sativa (rice)	Os02g0689800	NP_001047786.2
Arabidopsis thaliana (thale cress)	PIE1	NP_187887.3

Gene Ontology

The following LOC4330371 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC4330371 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOa28160	XM_015770121.2 Latest version!	Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOa28159	XM_015770118.2 Latest version!	Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OOa28159	XM_015770119.1 Latest version!	Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OOa45722	XM_026022821.1 Latest version!	Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOa45722	XM_026022820.1 Latest version!	Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OOa28160	XM_015770121.1	Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOa28159	XM_015770118.1	Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC4330371 ( XM_015770121.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015625607.1 Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOa28160
Clone ID Related Accession (Same CDS sequence)	XM_015770121.1 , XM_015770121.2
Accession Version	XM_015770121.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379175.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGG ATGATGAGTC TGCAGACACC GTGGAGGTAA ACAGGGATCT TTCTGCAGAT 
ACCATGAAAC TGACCAGAGA TCAGTCTGCA GAGACCGTAA AAGTGAATAA TGACCAGTCT 
GCAGAGATTG TGGAGGGCAA CAATGACACA TTTGAAGATC ATGAATCTGC AGGCATGCTG 
GGACCTGAGC ATGTCAGTGG TAGTGTTCTA CAACTAGAAA CATCAGAGCC TATTGTACAG 
GAAAATACTG CTAAAGAGGG TGATGTAACT GATACTAAAG CGATGGCCAA TGGAGATAAT 
AGTGATGTGA TTGCTGATGC TGCAGCTGCT GCAAGATCAG CACAACCGAC TGGTAACACG 
TTCTCAACAA CAAATGTGCG GACAAAATTC CCTTTCCTTC TTAAGCATTC TCTACGAGAA 
TATCAGCACA TTGGACTGGA CTGGCTTGTT GCTATGTATG AAAAGAGGCT GAATGGAATT 
CTAGCAGATG AAATGGGTTT GGGGAAGACA ATTATGACCA TCTCCTTGCT AGCACACCTT 
GCATGTGAGA AGGGAATATG GGGTCCACAT CTTATTGTAG TGCCAACTAG TGTTATGCTA 
AATTGGGAGA CTGAATTTCT CAAATGGTGT CCTGCGTTTA AAATACTGAC ATATTTTGGA 
AGTGCAAAAG AGAGAAAACA GAAACGTCAA GGTTGGATGA AACCAAATTA CTTCCATGTA 
TGCATCACTA CATACAGGCT TGTTATTCAG GACTCCAAAG TGTTTAAGCG AAAAAAATGG 
AAGTATCTTA TTCTTGATGA GGCTCATTTG ATAAAGAACT GGAAATCACA AAGATGGCAG 
ACTTTACTAA ATTTTAATTC GAAGCGACGC ATTCTTTTAA CTGGAACTCC TCTACAAAAT 
GACCTCATGG AACTTTGGTC TCTCATGCAC TTTTTGATGC CTCATGTATT TCAGTCACAT 
CAAGAGTTCA AGGACTGGTT CTGCAATCCA ATTTCAGGCA TGGTAGAGGG CCAAGACAAG 
GTAAACAAAG AAGTGATAGA TCGGTTGCAC AACGTCCTTC GTCCGTTTAT ACTTCGTCGA 
TTGAAACGGG ATGTGGAGAA ACAGTTGCCA CAGAAGCATG AGCATGTCAT ATATTGCCGA 
CTTTCCAGAA GGCAAAGGAA CTTGTATGAA GATTTCATTG CAAGCTCAGA GACACAGGCA 
ACATTGGCAA GTGGAAATTA TTTTGGCATG ATTAGCATCA TTATGCAGCT TAGAAAGGTT 
TGTAACCATC CAGATCTTTT TGAAGGCCGT CCAATTATCA GCTCTTTTGA CATGGCAGGG 
ATCAACATGC AAATCAGCTC TTCTGTTGGC ATGGTCCTTG ATAAAGGCCC ATTTTCTCAG 
GTTGACCTGT CAGATATGAA TTTTGTATTT ACTCAAAATG AATATAATAT GACTTCTTGG 
GAGGAAGATG AGGTTGCTGC CATTTTTTCT CCAAGCATAA CCCTCAGGGG TTCTGGCATT 
TCTCGCTCTA CTAATGATGG TCAGAGAAGC AATGGGACAA ATATTTTTGA AGAGATTCAC 
AATTCTTTGT GGGAGGAGAG AATAAAAGAA GCTAATGAAA GAGCAGCTTC CATTGCATGG 
TGGAATAGGG TAAGATGCCA GAAGAGGCCT GTTTATGGCA CAAATATTCG GGAGGTTTTG 
ACCATAAAGC ATCCTGTGTC TGATATTCTC GAGAAGAAGA AGAACCCTTT ATGCCACATG 
GAATTTTCAT CAAGCCTAGC AAATCTTGTT CTATCATCGG TGGATCGCTT CAAGACAATG 
CTTGATTTTA TTGAGTCATT TACTTTTGCA ATTCCTGCTG CACGAGCGCC TGCACCATTA 
TTCTGGTGCA ACAAAGGGAA ATCTCCTGTT CTTATTGAAC CAGCATACAG AGAGAAATGC 
ATGAATGAGT TTTCTCCTGT TTTCTCTCCT ATAAGGCCTG CGATTGTTCG CCGTCAAGTG 
TACTTTCCTG ACAGGCGTTT GATCCAGTTT GACTGTGGAA AGTTGCAGGA ACTTGCTATT 
TTGTTGAGGC GTTTGAAGTC AGAAGGACAC AGGGCCTTGA TATTTACTCA GATGACTAAG 
ATGCTTGATA TACTGGAGGA GTTCATTAAT CTATATGGGT ATACGTATTT GCGGTTAGAT 
GGATCAACCC AGCCAGAAGA GAGGCAGACA CTGATGCAGA GGTTTAATAC TAACCCAAAG 
TTTTTTCTTT TTATCTTATC CACTCGTAGT GGTGGTGTGG GAATCAACCT AGTAGGTGCA 
GACACTGTCA TTTTCTATGA TAGTGACTGG AATCCTGCAA TGGACCAACA GGCCCAAGAC 
AGATGCCACA GGATAGGTCA AACTCGTGAA GTTCACATCT ATAGACTGAT CAGTGAGAGC 
ACTATAGAGG AGAATATACT CAAGAAAGCC AATCAGAAGC GAGCTCTTGA TGATCTAGTG 
ATACAACGCG GTAGCTACAA TACAGAGTTC TTCAAGAAGC TTGATCCTAT GGAATTCTTT 
TCTGGGCACT CATCACTTCA TGCAGAGAAT CAGCAGAAGG ATTGCTCTCT GTCTGCTGGA 
CCCTCAAACG GAACTAATCT GGCGCTGTCA AATGTTGATG TTGAAGCAGC TATTAGACAG 
GCAGAAGATG AAGCTGACTA TATGGCTCTC AAGAGGCTGG AGCAGGAAGA GGCCGTGGAC 
AATCAAGAGT TCAGCGAGGA GGCTGCTGGA AGACTTGAGG AGGATGATCT TGTAAACGAG 
GATGACACTA AACCTGATGA ACACACCAAT GAAGAGCATA AATATCAGTG TTCTGATTTG 
GTCAAGGACA AGCATGTTGC TTTATCTATA AATCAATTAG ATGAAGAAAA GGCTATAACT 
TTGGCTGGAG GAGATGGAGA TATTGACATG CTTGCTGATG TTAAGCAGAT GGCTGCTGCA 
GCTGCTGCAG CAGGTCAAGC AAGTTCGTCT TTTGAAAATC AGCTCCGGCC AATTGATAGA 
TATGCCATGC GGTTTTTGGA ACTCTGGGAT CCAATAATTG ATAAAGCTGC TATAAACTAT 
CAGGTCAATG TTGAAGAGGA AGAATGGGAA CTTGAACGCA TTGAAAAACT CAAAGAAGAT 
TTAGAAGCAG AAATTGATGA AGACCAGGAA CCACTTTCTT ATGAGTCATG GGATGTTGAT 
TTTGCTACTA CAGCATATCG GCAACATGTT GAGGCTCTAG CTCAAAAACA GTTGTTTGAG 
GAACAAGAAA GACAAGCCCG TGAAGCAGCA AAAGAGTTGG AGGAAAAGAA TGATAATAGC 
ACTCAACGAA AAAAGTCAAA AAAGAACAAG AAGAAGGCAG CGAAGTTCAA ATCTTTGAAG 
AAAGGACGTT TATCATCTGA GTCAGAGGTT ATGGTTGAGG AAACATCAGT AGATACTATG 
AGCATTGATG ACAATGCACC CTCACCAGAG CTAATGAGCG ATGAATCAGC ACACCATCAT 
TCTAACAAGC ATAAGAGGAT AATGTCAACC AATGAGGAAG AGAACAGCAA TAGCAGAAGC 
CTGAAGAAGC TCAAGAAAGC CCCTAAATCG AGTTTCAGTT CCGAGGCCTT ATCACCTAAG 
CATTTTCTTG AAGGCAAGCA GCTTAAGTTG AAAGATGAAC TTAATGATTC TGATCCCAAA 
TCGGGGGCAA GAATTAAGAG TGATGGCCGA ATTTCTGTCC CATGCATGCC AGTAAAGCGT 
GTCATGGTAA TCAAGCCTGA GAGATTGAAG AAGAAGAGCC TATGGTCTCG CGATTGTGCT 
TCAGACTCGT GGACAACTGA GGAAGATGCT GTTCTTTGTG CAACTGTAAA TGAGTACGGC 
CCTCTTTGGG AACTGGCTAG TGATTCTCTT CATTCTGTAC CAGGTGGTGC ATTTTATAGA 
GGAAGATACC GTCACCCTGT GCATTGCTGT GAGAGATTCC GGGAATTAGT CTGCAAGCAC 
ATCTTATCAG CAACAGACAA TTCAAACAGT GAGAAGGTTC CTTCTGGGAC TGGGAAGGCT 
ATCTTGAAAG TATCTGAGGA TCAAACTCAG ATGTTGCTCA ATGTAATCAG CGAGCTTCCT 
AACAATGAAT TGCTTCTCCA GAAACATTTC ATGGCTGTTC TTTCATCTGT TTGGAGATCG 
AAGTCTGCCT GTGAGTCCCA CTGTGTTATG AGTTACTCTA ACACACTCCA GAAACCTGGC 
AGACTCAGCG AGAACTGGTC CATGACAAAC TTCAGACCAA ACTTTAATCT AGTAAGGACA 
GCCCTCGCAG ATGCTCAGGT TCAATGTCCA AGAATGGTAG TTCCAACAAG CAATCATGAG 
TCCCGCCGTA ACTTTTTGGA ATTGGAATTG GATTTCTTGA CAGATCGAGA TGATTATGAG 
GCGGACTTTC CATCTGTTGT TAACGTGTCC ATACTAGAAC CGGAACCATT AAAACATGCT 
GTGGAGCCAG TGGAGCACTC ACTACTGTCC ACACTTTCTT ATAGACATGC TGAGAACAGA 
TTCAGGATGG TATCAGAAAC TTGTTTTGAA GGGGAAGGTT CTCACTGGGC CTCATCTGCT 
TTCCACACAT ACGATGCCGG TCGGCATAAA TCTGGTCCTA AGTCCATAGG AAAGCACAAA 
GCATCATCAG AATCTGGAAG GCCTCCCAAA TCCAAAATTC AAAGGACCAC TGAACCACAG 
GAAGTGCCGG TTACAAATAA TTTTCATAGG ATTCCGGGGC AACTTCTGCA TAACTCAGCA 
GAGTTCCACA TTACCCAGTC CCTATCTGAC CTTGGCATCA GTGATTCTGA ATTCACCTAC 
TTTGATAATC TTCCTCAAGA GGCTGAAACA GAGTTTGTTC CGTACCAGTA TGACTCTGAT 
GTCCTTTCTG GCATTGAGGA GTTGGATCCT CTTACAGATT TCACAGACAT CGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015625607.1
CDS	173..5089
Translation	MPVDDESADTVEVNRDLSADTMKLTRDQSAETVKVNNDQSAEIVEGNNDTFEDHESAGMLGPEHVSGSVLQLETSEPIVQENTAKEGDVTDTKAMANGDNSDVIADAAAAARSAQPTGNTFSTTNVRTKFPFLLKHSLREYQHIGLDWLVAMYEKRLNGILADEMGLGKTIMTISLLAHLACEKGIWGPHLIVVPTSVMLNWETEFLKWCPAFKILTYFGSAKERKQKRQGWMKPNYFHVCITTYRLVIQDSKVFKRKKWKYLILDEAHLIKNWKSQRWQTLLNFNSKRRILLTGTPLQNDLMELWSLMHFLMPHVFQSHQEFKDWFCNPISGMVEGQDKVNKEVIDRLHNVLRPFILRRLKRDVEKQLPQKHEHVIYCRLSRRQRNLYEDFIASSETQATLASGNYFGMISIIMQLRKVCNHPDLFEGRPIISSFDMAGINMQISSSVGMVLDKGPFSQVDLSDMNFVFTQNEYNMTSWEEDEVAAIFSPSITLRGSGISRSTNDGQRSNGTNIFEEIHNSLWEERIKEANERAASIAWWNRVRCQKRPVYGTNIREVLTIKHPVSDILEKKKNPLCHMEFSSSLANLVLSSVDRFKTMLDFIESFTFAIPAARAPAPLFWCNKGKSPVLIEPAYREKCMNEFSPVFSPIRPAIVRRQVYFPDRRLIQFDCGKLQELAILLRRLKSEGHRALIFTQMTKMLDILEEFINLYGYTYLRLDGSTQPEERQTLMQRFNTNPKFFLFILSTRSGGVGINLVGADTVIFYDSDWNPAMDQQAQDRCHRIGQTREVHIYRLISESTIEENILKKANQKRALDDLVIQRGSYNTEFFKKLDPMEFFSGHSSLHAENQQKDCSLSAGPSNGTNLALSNVDVEAAIRQAEDEADYMALKRLEQEEAVDNQEFSEEAAGRLEEDDLVNEDDTKPDEHTNEEHKYQCSDLVKDKHVALSINQLDEEKAITLAGGDGDIDMLADVKQMAAAAAAAGQASSSFENQLRPIDRYAMRFLELWDPIIDKAAINYQVNVEEEEWELERIEKLKEDLEAEIDEDQEPLSYESWDVDFATTAYRQHVEALAQKQLFEEQERQAREAAKELEEKNDNSTQRKKSKKNKKKAAKFKSLKKGRLSSESEVMVEETSVDTMSIDDNAPSPELMSDESAHHHSNKHKRIMSTNEEENSNSRSLKKLKKAPKSSFSSEALSPKHFLEGKQLKLKDELNDSDPKSGARIKSDGRISVPCMPVKRVMVIKPERLKKKSLWSRDCASDSWTTEEDAVLCATVNEYGPLWELASDSLHSVPGGAFYRGRYRHPVHCCERFRELVCKHILSATDNSNSEKVPSGTGKAILKVSEDQTQMLLNVISELPNNELLLQKHFMAVLSSVWRSKSACESHCVMSYSNTLQKPGRLSENWSMTNFRPNFNLVRTALADAQVQCPRMVVPTSNHESRRNFLELELDFLTDRDDYEADFPSVVNVSILEPEPLKHAVEPVEHSLLSTLSYRHAENRFRMVSETCFEGEGSHWASSAFHTYDAGRHKSGPKSIGKHKASSESGRPPKSKIQRTTEPQEVPVTNNFHRIPGQLLHNSAEFHITQSLSDLGISDSEFTYFDNLPQEAETEFVPYQYDSDVLSGIEELDPLTDFTDIG

Target ORF information:

RefSeq Version	XM_015770121.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015770121.1

ORF Insert Sequence:

ATGCCTGTGG ATGATGAGTC TGCAGACACC GTGGAGGTAA ACAGGGATCT TTCTGCAGAT 
ACCATGAAAC TGACCAGAGA TCAGTCTGCA GAGACCGTAA AAGTGAATAA TGACCAGTCT 
GCAGAGATTG TGGAGGGCAA CAATGACACA TTTGAAGATC ATGAATCTGC AGGCATGCTG 
GGACCTGAGC ATGTCAGTGG TAGTGTTCTA CAACTAGAAA CATCAGAGCC TATTGTACAG 
GAAAATACTG CTAAAGAGGG TGATGTAACT GATACTAAAG CGATGGCCAA TGGAGATAAT 
AGTGATGTGA TTGCTGATGC TGCAGCTGCT GCAAGATCAG CACAACCGAC TGGTAACACG 
TTCTCAACAA CAAATGTGCG GACAAAATTC CCTTTCCTTC TTAAGCATTC TCTACGAGAA 
TATCAGCACA TTGGACTGGA CTGGCTTGTT GCTATGTATG AAAAGAGGCT GAATGGAATT 
CTAGCAGATG AAATGGGTTT GGGGAAGACA ATTATGACCA TCTCCTTGCT AGCACACCTT 
GCATGTGAGA AGGGAATATG GGGTCCACAT CTTATTGTAG TGCCAACTAG TGTTATGCTA 
AATTGGGAGA CTGAATTTCT CAAATGGTGT CCTGCGTTTA AAATACTGAC ATATTTTGGA 
AGTGCAAAAG AGAGAAAACA GAAACGTCAA GGTTGGATGA AACCAAATTA CTTCCATGTA 
TGCATCACTA CATACAGGCT TGTTATTCAG GACTCCAAAG TGTTTAAGCG AAAAAAATGG 
AAGTATCTTA TTCTTGATGA GGCTCATTTG ATAAAGAACT GGAAATCACA AAGATGGCAG 
ACTTTACTAA ATTTTAATTC GAAGCGACGC ATTCTTTTAA CTGGAACTCC TCTACAAAAT 
GACCTCATGG AACTTTGGTC TCTCATGCAC TTTTTGATGC CTCATGTATT TCAGTCACAT 
CAAGAGTTCA AGGACTGGTT CTGCAATCCA ATTTCAGGCA TGGTAGAGGG CCAAGACAAG 
GTAAACAAAG AAGTGATAGA TCGGTTGCAC AACGTCCTTC GTCCGTTTAT ACTTCGTCGA 
TTGAAACGGG ATGTGGAGAA ACAGTTGCCA CAGAAGCATG AGCATGTCAT ATATTGCCGA 
CTTTCCAGAA GGCAAAGGAA CTTGTATGAA GATTTCATTG CAAGCTCAGA GACACAGGCA 
ACATTGGCAA GTGGAAATTA TTTTGGCATG ATTAGCATCA TTATGCAGCT TAGAAAGGTT 
TGTAACCATC CAGATCTTTT TGAAGGCCGT CCAATTATCA GCTCTTTTGA CATGGCAGGG 
ATCAACATGC AAATCAGCTC TTCTGTTGGC ATGGTCCTTG ATAAAGGCCC ATTTTCTCAG 
GTTGACCTGT CAGATATGAA TTTTGTATTT ACTCAAAATG AATATAATAT GACTTCTTGG 
GAGGAAGATG AGGTTGCTGC CATTTTTTCT CCAAGCATAA CCCTCAGGGG TTCTGGCATT 
TCTCGCTCTA CTAATGATGG TCAGAGAAGC AATGGGACAA ATATTTTTGA AGAGATTCAC 
AATTCTTTGT GGGAGGAGAG AATAAAAGAA GCTAATGAAA GAGCAGCTTC CATTGCATGG 
TGGAATAGGG TAAGATGCCA GAAGAGGCCT GTTTATGGCA CAAATATTCG GGAGGTTTTG 
ACCATAAAGC ATCCTGTGTC TGATATTCTC GAGAAGAAGA AGAACCCTTT ATGCCACATG 
GAATTTTCAT CAAGCCTAGC AAATCTTGTT CTATCATCGG TGGATCGCTT CAAGACAATG 
CTTGATTTTA TTGAGTCATT TACTTTTGCA ATTCCTGCTG CACGAGCGCC TGCACCATTA 
TTCTGGTGCA ACAAAGGGAA ATCTCCTGTT CTTATTGAAC CAGCATACAG AGAGAAATGC 
ATGAATGAGT TTTCTCCTGT TTTCTCTCCT ATAAGGCCTG CGATTGTTCG CCGTCAAGTG 
TACTTTCCTG ACAGGCGTTT GATCCAGTTT GACTGTGGAA AGTTGCAGGA ACTTGCTATT 
TTGTTGAGGC GTTTGAAGTC AGAAGGACAC AGGGCCTTGA TATTTACTCA GATGACTAAG 
ATGCTTGATA TACTGGAGGA GTTCATTAAT CTATATGGGT ATACGTATTT GCGGTTAGAT 
GGATCAACCC AGCCAGAAGA GAGGCAGACA CTGATGCAGA GGTTTAATAC TAACCCAAAG 
TTTTTTCTTT TTATCTTATC CACTCGTAGT GGTGGTGTGG GAATCAACCT AGTAGGTGCA 
GACACTGTCA TTTTCTATGA TAGTGACTGG AATCCTGCAA TGGACCAACA GGCCCAAGAC 
AGATGCCACA GGATAGGTCA AACTCGTGAA GTTCACATCT ATAGACTGAT CAGTGAGAGC 
ACTATAGAGG AGAATATACT CAAGAAAGCC AATCAGAAGC GAGCTCTTGA TGATCTAGTG 
ATACAACGCG GTAGCTACAA TACAGAGTTC TTCAAGAAGC TTGATCCTAT GGAATTCTTT 
TCTGGGCACT CATCACTTCA TGCAGAGAAT CAGCAGAAGG ATTGCTCTCT GTCTGCTGGA 
CCCTCAAACG GAACTAATCT GGCGCTGTCA AATGTTGATG TTGAAGCAGC TATTAGACAG 
GCAGAAGATG AAGCTGACTA TATGGCTCTC AAGAGGCTGG AGCAGGAAGA GGCCGTGGAC 
AATCAAGAGT TCAGCGAGGA GGCTGCTGGA AGACTTGAGG AGGATGATCT TGTAAACGAG 
GATGACACTA AACCTGATGA ACACACCAAT GAAGAGCATA AATATCAGTG TTCTGATTTG 
GTCAAGGACA AGCATGTTGC TTTATCTATA AATCAATTAG ATGAAGAAAA GGCTATAACT 
TTGGCTGGAG GAGATGGAGA TATTGACATG CTTGCTGATG TTAAGCAGAT GGCTGCTGCA 
GCTGCTGCAG CAGGTCAAGC AAGTTCGTCT TTTGAAAATC AGCTCCGGCC AATTGATAGA 
TATGCCATGC GGTTTTTGGA ACTCTGGGAT CCAATAATTG ATAAAGCTGC TATAAACTAT 
CAGGTCAATG TTGAAGAGGA AGAATGGGAA CTTGAACGCA TTGAAAAACT CAAAGAAGAT 
TTAGAAGCAG AAATTGATGA AGACCAGGAA CCACTTTCTT ATGAGTCATG GGATGTTGAT 
TTTGCTACTA CAGCATATCG GCAACATGTT GAGGCTCTAG CTCAAAAACA GTTGTTTGAG 
GAACAAGAAA GACAAGCCCG TGAAGCAGCA AAAGAGTTGG AGGAAAAGAA TGATAATAGC 
ACTCAACGAA AAAAGTCAAA AAAGAACAAG AAGAAGGCAG CGAAGTTCAA ATCTTTGAAG 
AAAGGACGTT TATCATCTGA GTCAGAGGTT ATGGTTGAGG AAACATCAGT AGATACTATG 
AGCATTGATG ACAATGCACC CTCACCAGAG CTAATGAGCG ATGAATCAGC ACACCATCAT 
TCTAACAAGC ATAAGAGGAT AATGTCAACC AATGAGGAAG AGAACAGCAA TAGCAGAAGC 
CTGAAGAAGC TCAAGAAAGC CCCTAAATCG AGTTTCAGTT CCGAGGCCTT ATCACCTAAG 
CATTTTCTTG AAGGCAAGCA GCTTAAGTTG AAAGATGAAC TTAATGATTC TGATCCCAAA 
TCGGGGGCAA GAATTAAGAG TGATGGCCGA ATTTCTGTCC CATGCATGCC AGTAAAGCGT 
GTCATGGTAA TCAAGCCTGA GAGATTGAAG AAGAAGAGCC TATGGTCTCG CGATTGTGCT 
TCAGACTCGT GGACAACTGA GGAAGATGCT GTTCTTTGTG CAACTGTAAA TGAGTACGGC 
CCTCTTTGGG AACTGGCTAG TGATTCTCTT CATTCTGTAC CAGGTGGTGC ATTTTATAGA 
GGAAGATACC GTCACCCTGT GCATTGCTGT GAGAGATTCC GGGAATTAGT CTGCAAGCAC 
ATCTTATCAG CAACAGACAA TTCAAACAGT GAGAAGGTTC CTTCTGGGAC TGGGAAGGCT 
ATCTTGAAAG TATCTGAGGA TCAAACTCAG ATGTTGCTCA ATGTAATCAG CGAGCTTCCT 
AACAATGAAT TGCTTCTCCA GAAACATTTC ATGGCTGTTC TTTCATCTGT TTGGAGATCG 
AAGTCTGCCT GTGAGTCCCA CTGTGTTATG AGTTACTCTA ACACACTCCA GAAACCTGGC 
AGACTCAGCG AGAACTGGTC CATGACAAAC TTCAGACCAA ACTTTAATCT AGTAAGGACA 
GCCCTCGCAG ATGCTCAGGT TCAATGTCCA AGAATGGTAG TTCCAACAAG CAATCATGAG 
TCCCGCCGTA ACTTTTTGGA ATTGGAATTG GATTTCTTGA CAGATCGAGA TGATTATGAG 
GCGGACTTTC CATCTGTTGT TAACGTGTCC ATACTAGAAC CGGAACCATT AAAACATGCT 
GTGGAGCCAG TGGAGCACTC ACTACTGTCC ACACTTTCTT ATAGACATGC TGAGAACAGA 
TTCAGGATGG TATCAGAAAC TTGTTTTGAA GGGGAAGGTT CTCACTGGGC CTCATCTGCT 
TTCCACACAT ACGATGCCGG TCGGCATAAA TCTGGTCCTA AGTCCATAGG AAAGCACAAA 
GCATCATCAG AATCTGGAAG GCCTCCCAAA TCCAAAATTC AAAGGACCAC TGAACCACAG 
GAAGTGCCGG TTACAAATAA TTTTCATAGG ATTCCGGGGC AACTTCTGCA TAACTCAGCA 
GAGTTCCACA TTACCCAGTC CCTATCTGAC CTTGGCATCA GTGATTCTGA ATTCACCTAC 
TTTGATAATC TTCCTCAAGA GGCTGAAACA GAGTTTGTTC CGTACCAGTA TGACTCTGAT 
GTCCTTTCTG GCATTGAGGA GTTGGATCCT CTTACAGATT TCACAGACAT CGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4330371 ( XM_015770121.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015625607.1 Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X5, mRNA.

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CloneID	OOa28160
Clone ID Related Accession (Same CDS sequence)	XM_015770121.1 , XM_015770121.2
Accession Version	XM_015770121.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029257.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015770121.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGG ATGATGAGTC TGCAGACACC GTGGAGGTAA ACAGGGATCT TTCTGCAGAT 
ACCATGAAAC TGACCAGAGA TCAGTCTGCA GAGACCGTAA AAGTGAATAA TGACCAGTCT 
GCAGAGATTG TGGAGGGCAA CAATGACACA TTTGAAGATC ATGAATCTGC AGGCATGCTG 
GGACCTGAGC ATGTCAGTGG TAGTGTTCTA CAACTAGAAA CATCAGAGCC TATTGTACAG 
GAAAATACTG CTAAAGAGGG TGATGTAACT GATACTAAAG CGATGGCCAA TGGAGATAAT 
AGTGATGTGA TTGCTGATGC TGCAGCTGCT GCAAGATCAG CACAACCGAC TGGTAACACG 
TTCTCAACAA CAAATGTGCG GACAAAATTC CCTTTCCTTC TTAAGCATTC TCTACGAGAA 
TATCAGCACA TTGGACTGGA CTGGCTTGTT GCTATGTATG AAAAGAGGCT GAATGGAATT 
CTAGCAGATG AAATGGGTTT GGGGAAGACA ATTATGACCA TCTCCTTGCT AGCACACCTT 
GCATGTGAGA AGGGAATATG GGGTCCACAT CTTATTGTAG TGCCAACTAG TGTTATGCTA 
AATTGGGAGA CTGAATTTCT CAAATGGTGT CCTGCGTTTA AAATACTGAC ATATTTTGGA 
AGTGCAAAAG AGAGAAAACA GAAACGTCAA GGTTGGATGA AACCAAATTA CTTCCATGTA 
TGCATCACTA CATACAGGCT TGTTATTCAG GACTCCAAAG TGTTTAAGCG AAAAAAATGG 
AAGTATCTTA TTCTTGATGA GGCTCATTTG ATAAAGAACT GGAAATCACA AAGATGGCAG 
ACTTTACTAA ATTTTAATTC GAAGCGACGC ATTCTTTTAA CTGGAACTCC TCTACAAAAT 
GACCTCATGG AACTTTGGTC TCTCATGCAC TTTTTGATGC CTCATGTATT TCAGTCACAT 
CAAGAGTTCA AGGACTGGTT CTGCAATCCA ATTTCAGGCA TGGTAGAGGG CCAAGACAAG 
GTAAACAAAG AAGTGATAGA TCGGTTGCAC AACGTCCTTC GTCCGTTTAT ACTTCGTCGA 
TTGAAACGGG ATGTGGAGAA ACAGTTGCCA CAGAAGCATG AGCATGTCAT ATATTGCCGA 
CTTTCCAGAA GGCAAAGGAA CTTGTATGAA GATTTCATTG CAAGCTCAGA GACACAGGCA 
ACATTGGCAA GTGGAAATTA TTTTGGCATG ATTAGCATCA TTATGCAGCT TAGAAAGGTT 
TGTAACCATC CAGATCTTTT TGAAGGCCGT CCAATTATCA GCTCTTTTGA CATGGCAGGG 
ATCAACATGC AAATCAGCTC TTCTGTTGGC ATGGTCCTTG ATAAAGGCCC ATTTTCTCAG 
GTTGACCTGT CAGATATGAA TTTTGTATTT ACTCAAAATG AATATAATAT GACTTCTTGG 
GAGGAAGATG AGGTTGCTGC CATTTTTTCT CCAAGCATAA CCCTCAGGGG TTCTGGCATT 
TCTCGCTCTA CTAATGATGG TCAGAGAAGC AATGGGACAA ATATTTTTGA AGAGATTCAC 
AATTCTTTGT GGGAGGAGAG AATAAAAGAA GCTAATGAAA GAGCAGCTTC CATTGCATGG 
TGGAATAGGG TAAGATGCCA GAAGAGGCCT GTTTATGGCA CAAATATTCG GGAGGTTTTG 
ACCATAAAGC ATCCTGTGTC TGATATTCTC GAGAAGAAGA AGAACCCTTT ATGCCACATG 
GAATTTTCAT CAAGCCTAGC AAATCTTGTT CTATCATCGG TGGATCGCTT CAAGACAATG 
CTTGATTTTA TTGAGTCATT TACTTTTGCA ATTCCTGCTG CACGAGCGCC TGCACCATTA 
TTCTGGTGCA ACAAAGGGAA ATCTCCTGTT CTTATTGAAC CAGCATACAG AGAGAAATGC 
ATGAATGAGT TTTCTCCTGT TTTCTCTCCT ATAAGGCCTG CGATTGTTCG CCGTCAAGTG 
TACTTTCCTG ACAGGCGTTT GATCCAGTTT GACTGTGGAA AGTTGCAGGA ACTTGCTATT 
TTGTTGAGGC GTTTGAAGTC AGAAGGACAC AGGGCCTTGA TATTTACTCA GATGACTAAG 
ATGCTTGATA TACTGGAGGA GTTCATTAAT CTATATGGGT ATACGTATTT GCGGTTAGAT 
GGATCAACCC AGCCAGAAGA GAGGCAGACA CTGATGCAGA GGTTTAATAC TAACCCAAAG 
TTTTTTCTTT TTATCTTATC CACTCGTAGT GGTGGTGTGG GAATCAACCT AGTAGGTGCA 
GACACTGTCA TTTTCTATGA TAGTGACTGG AATCCTGCAA TGGACCAACA GGCCCAAGAC 
AGATGCCACA GGATAGGTCA AACTCGTGAA GTTCACATCT ATAGACTGAT CAGTGAGAGC 
ACTATAGAGG AGAATATACT CAAGAAAGCC AATCAGAAGC GAGCTCTTGA TGATCTAGTG 
ATACAACGCG GTAGCTACAA TACAGAGTTC TTCAAGAAGC TTGATCCTAT GGAATTCTTT 
TCTGGGCACT CATCACTTCA TGCAGAGAAT CAGCAGAAGG ATTGCTCTCT GTCTGCTGGA 
CCCTCAAACG GAACTAATCT GGCGCTGTCA AATGTTGATG TTGAAGCAGC TATTAGACAG 
GCAGAAGATG AAGCTGACTA TATGGCTCTC AAGAGGCTGG AGCAGGAAGA GGCCGTGGAC 
AATCAAGAGT TCAGCGAGGA GGCTGCTGGA AGACTTGAGG AGGATGATCT TGTAAACGAG 
GATGACACTA AACCTGATGA ACACACCAAT GAAGAGCATA AATATCAGTG TTCTGATTTG 
GTCAAGGACA AGCATGTTGC TTTATCTATA AATCAATTAG ATGAAGAAAA GGCTATAACT 
TTGGCTGGAG GAGATGGAGA TATTGACATG CTTGCTGATG TTAAGCAGAT GGCTGCTGCA 
GCTGCTGCAG CAGGTCAAGC AAGTTCGTCT TTTGAAAATC AGCTCCGGCC AATTGATAGA 
TATGCCATGC GGTTTTTGGA ACTCTGGGAT CCAATAATTG ATAAAGCTGC TATAAACTAT 
CAGGTCAATG TTGAAGAGGA AGAATGGGAA CTTGAACGCA TTGAAAAACT CAAAGAAGAT 
TTAGAAGCAG AAATTGATGA AGACCAGGAA CCACTTTCTT ATGAGTCATG GGATGTTGAT 
TTTGCTACTA CAGCATATCG GCAACATGTT GAGGCTCTAG CTCAAAAACA GTTGTTTGAG 
GAACAAGAAA GACAAGCCCG TGAAGCAGCA AAAGAGTTGG AGGAAAAGAA TGATAATAGC 
ACTCAACGAA AAAAGTCAAA AAAGAACAAG AAGAAGGCAG CGAAGTTCAA ATCTTTGAAG 
AAAGGACGTT TATCATCTGA GTCAGAGGTT ATGGTTGAGG AAACATCAGT AGATACTATG 
AGCATTGATG ACAATGCACC CTCACCAGAG CTAATGAGCG ATGAATCAGC ACACCATCAT 
TCTAACAAGC ATAAGAGGAT AATGTCAACC AATGAGGAAG AGAACAGCAA TAGCAGAAGC 
CTGAAGAAGC TCAAGAAAGC CCCTAAATCG AGTTTCAGTT CCGAGGCCTT ATCACCTAAG 
CATTTTCTTG AAGGCAAGCA GCTTAAGTTG AAAGATGAAC TTAATGATTC TGATCCCAAA 
TCGGGGGCAA GAATTAAGAG TGATGGCCGA ATTTCTGTCC CATGCATGCC AGTAAAGCGT 
GTCATGGTAA TCAAGCCTGA GAGATTGAAG AAGAAGAGCC TATGGTCTCG CGATTGTGCT 
TCAGACTCGT GGACAACTGA GGAAGATGCT GTTCTTTGTG CAACTGTAAA TGAGTACGGC 
CCTCTTTGGG AACTGGCTAG TGATTCTCTT CATTCTGTAC CAGGTGGTGC ATTTTATAGA 
GGAAGATACC GTCACCCTGT GCATTGCTGT GAGAGATTCC GGGAATTAGT CTGCAAGCAC 
ATCTTATCAG CAACAGACAA TTCAAACAGT GAGAAGGTTC CTTCTGGGAC TGGGAAGGCT 
ATCTTGAAAG TATCTGAGGA TCAAACTCAG ATGTTGCTCA ATGTAATCAG CGAGCTTCCT 
AACAATGAAT TGCTTCTCCA GAAACATTTC ATGGCTGTTC TTTCATCTGT TTGGAGATCG 
AAGTCTGCCT GTGAGTCCCA CTGTGTTATG AGTTACTCTA ACACACTCCA GAAACCTGGC 
AGACTCAGCG AGAACTGGTC CATGACAAAC TTCAGACCAA ACTTTAATCT AGTAAGGACA 
GCCCTCGCAG ATGCTCAGGT TCAATGTCCA AGAATGGTAG TTCCAACAAG CAATCATGAG 
TCCCGCCGTA ACTTTTTGGA ATTGGAATTG GATTTCTTGA CAGATCGAGA TGATTATGAG 
GCGGACTTTC CATCTGTTGT TAACGTGTCC ATACTAGAAC CGGAACCATT AAAACATGCT 
GTGGAGCCAG TGGAGCACTC ACTACTGTCC ACACTTTCTT ATAGACATGC TGAGAACAGA 
TTCAGGATGG TATCAGAAAC TTGTTTTGAA GGGGAAGGTT CTCACTGGGC CTCATCTGCT 
TTCCACACAT ACGATGCCGG TCGGCATAAA TCTGGTCCTA AGTCCATAGG AAAGCACAAA 
GCATCATCAG AATCTGGAAG GCCTCCCAAA TCCAAAATTC AAAGGACCAC TGAACCACAG 
GAAGTGCCGG TTACAAATAA TTTTCATAGG ATTCCGGGGC AACTTCTGCA TAACTCAGCA 
GAGTTCCACA TTACCCAGTC CCTATCTGAC CTTGGCATCA GTGATTCTGA ATTCACCTAC 
TTTGATAATC TTCCTCAAGA GGCTGAAACA GAGTTTGTTC CGTACCAGTA TGACTCTGAT 
GTCCTTTCTG GCATTGAGGA GTTGGATCCT CTTACAGATT TCACAGACAT CGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015625607.1
CDS	194..5110
Translation	MPVDDESADTVEVNRDLSADTMKLTRDQSAETVKVNNDQSAEIVEGNNDTFEDHESAGMLGPEHVSGSVLQLETSEPIVQENTAKEGDVTDTKAMANGDNSDVIADAAAAARSAQPTGNTFSTTNVRTKFPFLLKHSLREYQHIGLDWLVAMYEKRLNGILADEMGLGKTIMTISLLAHLACEKGIWGPHLIVVPTSVMLNWETEFLKWCPAFKILTYFGSAKERKQKRQGWMKPNYFHVCITTYRLVIQDSKVFKRKKWKYLILDEAHLIKNWKSQRWQTLLNFNSKRRILLTGTPLQNDLMELWSLMHFLMPHVFQSHQEFKDWFCNPISGMVEGQDKVNKEVIDRLHNVLRPFILRRLKRDVEKQLPQKHEHVIYCRLSRRQRNLYEDFIASSETQATLASGNYFGMISIIMQLRKVCNHPDLFEGRPIISSFDMAGINMQISSSVGMVLDKGPFSQVDLSDMNFVFTQNEYNMTSWEEDEVAAIFSPSITLRGSGISRSTNDGQRSNGTNIFEEIHNSLWEERIKEANERAASIAWWNRVRCQKRPVYGTNIREVLTIKHPVSDILEKKKNPLCHMEFSSSLANLVLSSVDRFKTMLDFIESFTFAIPAARAPAPLFWCNKGKSPVLIEPAYREKCMNEFSPVFSPIRPAIVRRQVYFPDRRLIQFDCGKLQELAILLRRLKSEGHRALIFTQMTKMLDILEEFINLYGYTYLRLDGSTQPEERQTLMQRFNTNPKFFLFILSTRSGGVGINLVGADTVIFYDSDWNPAMDQQAQDRCHRIGQTREVHIYRLISESTIEENILKKANQKRALDDLVIQRGSYNTEFFKKLDPMEFFSGHSSLHAENQQKDCSLSAGPSNGTNLALSNVDVEAAIRQAEDEADYMALKRLEQEEAVDNQEFSEEAAGRLEEDDLVNEDDTKPDEHTNEEHKYQCSDLVKDKHVALSINQLDEEKAITLAGGDGDIDMLADVKQMAAAAAAAGQASSSFENQLRPIDRYAMRFLELWDPIIDKAAINYQVNVEEEEWELERIEKLKEDLEAEIDEDQEPLSYESWDVDFATTAYRQHVEALAQKQLFEEQERQAREAAKELEEKNDNSTQRKKSKKNKKKAAKFKSLKKGRLSSESEVMVEETSVDTMSIDDNAPSPELMSDESAHHHSNKHKRIMSTNEEENSNSRSLKKLKKAPKSSFSSEALSPKHFLEGKQLKLKDELNDSDPKSGARIKSDGRISVPCMPVKRVMVIKPERLKKKSLWSRDCASDSWTTEEDAVLCATVNEYGPLWELASDSLHSVPGGAFYRGRYRHPVHCCERFRELVCKHILSATDNSNSEKVPSGTGKAILKVSEDQTQMLLNVISELPNNELLLQKHFMAVLSSVWRSKSACESHCVMSYSNTLQKPGRLSENWSMTNFRPNFNLVRTALADAQVQCPRMVVPTSNHESRRNFLELELDFLTDRDDYEADFPSVVNVSILEPEPLKHAVEPVEHSLLSTLSYRHAENRFRMVSETCFEGEGSHWASSAFHTYDAGRHKSGPKSIGKHKASSESGRPPKSKIQRTTEPQEVPVTNNFHRIPGQLLHNSAEFHITQSLSDLGISDSEFTYFDNLPQEAETEFVPYQYDSDVLSGIEELDPLTDFTDIG

Target ORF information:

RefSeq Version	XM_015770121.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015770121.2

ORF Insert Sequence:

ATGCCTGTGG ATGATGAGTC TGCAGACACC GTGGAGGTAA ACAGGGATCT TTCTGCAGAT 
ACCATGAAAC TGACCAGAGA TCAGTCTGCA GAGACCGTAA AAGTGAATAA TGACCAGTCT 
GCAGAGATTG TGGAGGGCAA CAATGACACA TTTGAAGATC ATGAATCTGC AGGCATGCTG 
GGACCTGAGC ATGTCAGTGG TAGTGTTCTA CAACTAGAAA CATCAGAGCC TATTGTACAG 
GAAAATACTG CTAAAGAGGG TGATGTAACT GATACTAAAG CGATGGCCAA TGGAGATAAT 
AGTGATGTGA TTGCTGATGC TGCAGCTGCT GCAAGATCAG CACAACCGAC TGGTAACACG 
TTCTCAACAA CAAATGTGCG GACAAAATTC CCTTTCCTTC TTAAGCATTC TCTACGAGAA 
TATCAGCACA TTGGACTGGA CTGGCTTGTT GCTATGTATG AAAAGAGGCT GAATGGAATT 
CTAGCAGATG AAATGGGTTT GGGGAAGACA ATTATGACCA TCTCCTTGCT AGCACACCTT 
GCATGTGAGA AGGGAATATG GGGTCCACAT CTTATTGTAG TGCCAACTAG TGTTATGCTA 
AATTGGGAGA CTGAATTTCT CAAATGGTGT CCTGCGTTTA AAATACTGAC ATATTTTGGA 
AGTGCAAAAG AGAGAAAACA GAAACGTCAA GGTTGGATGA AACCAAATTA CTTCCATGTA 
TGCATCACTA CATACAGGCT TGTTATTCAG GACTCCAAAG TGTTTAAGCG AAAAAAATGG 
AAGTATCTTA TTCTTGATGA GGCTCATTTG ATAAAGAACT GGAAATCACA AAGATGGCAG 
ACTTTACTAA ATTTTAATTC GAAGCGACGC ATTCTTTTAA CTGGAACTCC TCTACAAAAT 
GACCTCATGG AACTTTGGTC TCTCATGCAC TTTTTGATGC CTCATGTATT TCAGTCACAT 
CAAGAGTTCA AGGACTGGTT CTGCAATCCA ATTTCAGGCA TGGTAGAGGG CCAAGACAAG 
GTAAACAAAG AAGTGATAGA TCGGTTGCAC AACGTCCTTC GTCCGTTTAT ACTTCGTCGA 
TTGAAACGGG ATGTGGAGAA ACAGTTGCCA CAGAAGCATG AGCATGTCAT ATATTGCCGA 
CTTTCCAGAA GGCAAAGGAA CTTGTATGAA GATTTCATTG CAAGCTCAGA GACACAGGCA 
ACATTGGCAA GTGGAAATTA TTTTGGCATG ATTAGCATCA TTATGCAGCT TAGAAAGGTT 
TGTAACCATC CAGATCTTTT TGAAGGCCGT CCAATTATCA GCTCTTTTGA CATGGCAGGG 
ATCAACATGC AAATCAGCTC TTCTGTTGGC ATGGTCCTTG ATAAAGGCCC ATTTTCTCAG 
GTTGACCTGT CAGATATGAA TTTTGTATTT ACTCAAAATG AATATAATAT GACTTCTTGG 
GAGGAAGATG AGGTTGCTGC CATTTTTTCT CCAAGCATAA CCCTCAGGGG TTCTGGCATT 
TCTCGCTCTA CTAATGATGG TCAGAGAAGC AATGGGACAA ATATTTTTGA AGAGATTCAC 
AATTCTTTGT GGGAGGAGAG AATAAAAGAA GCTAATGAAA GAGCAGCTTC CATTGCATGG 
TGGAATAGGG TAAGATGCCA GAAGAGGCCT GTTTATGGCA CAAATATTCG GGAGGTTTTG 
ACCATAAAGC ATCCTGTGTC TGATATTCTC GAGAAGAAGA AGAACCCTTT ATGCCACATG 
GAATTTTCAT CAAGCCTAGC AAATCTTGTT CTATCATCGG TGGATCGCTT CAAGACAATG 
CTTGATTTTA TTGAGTCATT TACTTTTGCA ATTCCTGCTG CACGAGCGCC TGCACCATTA 
TTCTGGTGCA ACAAAGGGAA ATCTCCTGTT CTTATTGAAC CAGCATACAG AGAGAAATGC 
ATGAATGAGT TTTCTCCTGT TTTCTCTCCT ATAAGGCCTG CGATTGTTCG CCGTCAAGTG 
TACTTTCCTG ACAGGCGTTT GATCCAGTTT GACTGTGGAA AGTTGCAGGA ACTTGCTATT 
TTGTTGAGGC GTTTGAAGTC AGAAGGACAC AGGGCCTTGA TATTTACTCA GATGACTAAG 
ATGCTTGATA TACTGGAGGA GTTCATTAAT CTATATGGGT ATACGTATTT GCGGTTAGAT 
GGATCAACCC AGCCAGAAGA GAGGCAGACA CTGATGCAGA GGTTTAATAC TAACCCAAAG 
TTTTTTCTTT TTATCTTATC CACTCGTAGT GGTGGTGTGG GAATCAACCT AGTAGGTGCA 
GACACTGTCA TTTTCTATGA TAGTGACTGG AATCCTGCAA TGGACCAACA GGCCCAAGAC 
AGATGCCACA GGATAGGTCA AACTCGTGAA GTTCACATCT ATAGACTGAT CAGTGAGAGC 
ACTATAGAGG AGAATATACT CAAGAAAGCC AATCAGAAGC GAGCTCTTGA TGATCTAGTG 
ATACAACGCG GTAGCTACAA TACAGAGTTC TTCAAGAAGC TTGATCCTAT GGAATTCTTT 
TCTGGGCACT CATCACTTCA TGCAGAGAAT CAGCAGAAGG ATTGCTCTCT GTCTGCTGGA 
CCCTCAAACG GAACTAATCT GGCGCTGTCA AATGTTGATG TTGAAGCAGC TATTAGACAG 
GCAGAAGATG AAGCTGACTA TATGGCTCTC AAGAGGCTGG AGCAGGAAGA GGCCGTGGAC 
AATCAAGAGT TCAGCGAGGA GGCTGCTGGA AGACTTGAGG AGGATGATCT TGTAAACGAG 
GATGACACTA AACCTGATGA ACACACCAAT GAAGAGCATA AATATCAGTG TTCTGATTTG 
GTCAAGGACA AGCATGTTGC TTTATCTATA AATCAATTAG ATGAAGAAAA GGCTATAACT 
TTGGCTGGAG GAGATGGAGA TATTGACATG CTTGCTGATG TTAAGCAGAT GGCTGCTGCA 
GCTGCTGCAG CAGGTCAAGC AAGTTCGTCT TTTGAAAATC AGCTCCGGCC AATTGATAGA 
TATGCCATGC GGTTTTTGGA ACTCTGGGAT CCAATAATTG ATAAAGCTGC TATAAACTAT 
CAGGTCAATG TTGAAGAGGA AGAATGGGAA CTTGAACGCA TTGAAAAACT CAAAGAAGAT 
TTAGAAGCAG AAATTGATGA AGACCAGGAA CCACTTTCTT ATGAGTCATG GGATGTTGAT 
TTTGCTACTA CAGCATATCG GCAACATGTT GAGGCTCTAG CTCAAAAACA GTTGTTTGAG 
GAACAAGAAA GACAAGCCCG TGAAGCAGCA AAAGAGTTGG AGGAAAAGAA TGATAATAGC 
ACTCAACGAA AAAAGTCAAA AAAGAACAAG AAGAAGGCAG CGAAGTTCAA ATCTTTGAAG 
AAAGGACGTT TATCATCTGA GTCAGAGGTT ATGGTTGAGG AAACATCAGT AGATACTATG 
AGCATTGATG ACAATGCACC CTCACCAGAG CTAATGAGCG ATGAATCAGC ACACCATCAT 
TCTAACAAGC ATAAGAGGAT AATGTCAACC AATGAGGAAG AGAACAGCAA TAGCAGAAGC 
CTGAAGAAGC TCAAGAAAGC CCCTAAATCG AGTTTCAGTT CCGAGGCCTT ATCACCTAAG 
CATTTTCTTG AAGGCAAGCA GCTTAAGTTG AAAGATGAAC TTAATGATTC TGATCCCAAA 
TCGGGGGCAA GAATTAAGAG TGATGGCCGA ATTTCTGTCC CATGCATGCC AGTAAAGCGT 
GTCATGGTAA TCAAGCCTGA GAGATTGAAG AAGAAGAGCC TATGGTCTCG CGATTGTGCT 
TCAGACTCGT GGACAACTGA GGAAGATGCT GTTCTTTGTG CAACTGTAAA TGAGTACGGC 
CCTCTTTGGG AACTGGCTAG TGATTCTCTT CATTCTGTAC CAGGTGGTGC ATTTTATAGA 
GGAAGATACC GTCACCCTGT GCATTGCTGT GAGAGATTCC GGGAATTAGT CTGCAAGCAC 
ATCTTATCAG CAACAGACAA TTCAAACAGT GAGAAGGTTC CTTCTGGGAC TGGGAAGGCT 
ATCTTGAAAG TATCTGAGGA TCAAACTCAG ATGTTGCTCA ATGTAATCAG CGAGCTTCCT 
AACAATGAAT TGCTTCTCCA GAAACATTTC ATGGCTGTTC TTTCATCTGT TTGGAGATCG 
AAGTCTGCCT GTGAGTCCCA CTGTGTTATG AGTTACTCTA ACACACTCCA GAAACCTGGC 
AGACTCAGCG AGAACTGGTC CATGACAAAC TTCAGACCAA ACTTTAATCT AGTAAGGACA 
GCCCTCGCAG ATGCTCAGGT TCAATGTCCA AGAATGGTAG TTCCAACAAG CAATCATGAG 
TCCCGCCGTA ACTTTTTGGA ATTGGAATTG GATTTCTTGA CAGATCGAGA TGATTATGAG 
GCGGACTTTC CATCTGTTGT TAACGTGTCC ATACTAGAAC CGGAACCATT AAAACATGCT 
GTGGAGCCAG TGGAGCACTC ACTACTGTCC ACACTTTCTT ATAGACATGC TGAGAACAGA 
TTCAGGATGG TATCAGAAAC TTGTTTTGAA GGGGAAGGTT CTCACTGGGC CTCATCTGCT 
TTCCACACAT ACGATGCCGG TCGGCATAAA TCTGGTCCTA AGTCCATAGG AAAGCACAAA 
GCATCATCAG AATCTGGAAG GCCTCCCAAA TCCAAAATTC AAAGGACCAC TGAACCACAG 
GAAGTGCCGG TTACAAATAA TTTTCATAGG ATTCCGGGGC AACTTCTGCA TAACTCAGCA 
GAGTTCCACA TTACCCAGTC CCTATCTGAC CTTGGCATCA GTGATTCTGA ATTCACCTAC 
TTTGATAATC TTCCTCAAGA GGCTGAAACA GAGTTTGTTC CGTACCAGTA TGACTCTGAT 
GTCCTTTCTG GCATTGAGGA GTTGGATCCT CTTACAGATT TCACAGACAT CGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4330371 ( XM_015770118.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015625604.1 Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOa28159
Clone ID Related Accession (Same CDS sequence)	XM_015770118.2 , XM_015770119.1 , XM_015770118.1
Accession Version	XM_015770118.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029257.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015770118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCAA AAGGTCCTCG ATCAAAGTTA GATCATGAGA CAAGAGCCAG ACGCCAGAAG 
GCTCTTGAAG CCCCAAGGGG GGAGCCTCGA AGGACGAAAA CTCACTGGGA CCATCTTCTT 
GGAGAAATGG CTTGGCTGGC AAAGGAGTTT GATGCTGAGA GAAAATGGAA GCTGTCCATG 
GCTAAAAGGA TTGCTCAAAG AGCTAATAAG GGTGTAGTTG ACCAAGCAAC AAAGGACGAG 
AGAAAACAGA AGGAGGAAGA AGTTCGCCTG AGAAAAGTTG CCCTAAATAT TTCTAAGGAC 
GTGAAGAAGT TCTGGACCAA AATAGAGAAG CTGGTTTTTT ATAAGAACCA ACTGGAGCTT 
GAGGAAAGGA AAAAAAAGGC CCTAGACAAG CAACTTGATT TCCTCTTAGG ACAGACTGAA 
AGATATTCAA CAATGCTGGC AGAAAATCTT GTTGATGTGC GCCTTCAAAA TCAAGAAAAT 
GACTCTTTAC AGACTAATCA AAGATCCCAG CAGGAACTAG CACAAGAGAA CATAAATGCA 
TCAAGTCCGA CTGATGTTGA CAATGTGGAA ATCGATGATG ATTACAACAG TTCCTTGGGT 
GAAGAGCCAA AAGATGATGA ACACACAATC GATGAGGATG AAGCTCAAAT TACGGAGGCT 
GAGCGCAACG AGGAATTGGC TGCTTTGCAG GCAGAAGCTG ATCTACCACT GGATGATATT 
CTCAAGTTGT ACACTAAAAA TAAAGTTAGC AGGGAAAGCA GTCCAGATGG CAGAGACGTT 
TTTAGTGACT CAGATTCAAA GGATTTGATT AAAGATCCTT TGAACCAGGC TAATGGATGC 
AACGATGAAT CTGATCATAC TTCAAGTGAT GAAGGCATTT CTTCTGAGGA AGCAGATGAT 
TACCAGTCTT ACTCTGAGTT TGTGAAAAAG AACACTGTGA AATGCAATGG CAATATCTCA 
TCTGTTGATG CAAAGGACGA CGAAGATTAT GTTGCTAATG ATGAAGGAAA GGATGATGAA 
GCTACTTTGT CTGAAGAGGA AGAGTTGGCG AAGAAAGAAG ACTGTGATCC TCTAGATGAG 
GTTAAGTTAC TGCAGAAAGA GAGTGAGATC CCACTTGAAG AACTTCTTGC AAGATACCAA 
AAGGATGGCT ATGCAGATGA TGACACAACA GAATTGGAAA ATTCACCTGC ACTATCAGTT 
GAGGATGTCA ATGCCAACAT GCCTGTGGAT GATGAGTCTG CAGACACCGT GGAGGTAAAC 
AGGGATCTTT CTGCAGATAC CATGAAACTG ACCAGAGATC AGTCTGCAGA GACCGTAAAA 
GTGAATAATG ACCAGTCTGC AGAGATTGTG GAGGGCAACA ATGACACATT TGAAGATCAT 
GAATCTGCAG GCATGCTGGG ACCTGAGCAT GTCAGTGGTA GTGTTCTACA ACTAGAAACA 
TCAGAGCCTA TTGTACAGGA AAATACTGCT AAAGAGGGTG ATGTAACTGA TACTAAAGCG 
ATGGCCAATG GAGATAATAG TGATGTGATT GCTGATGCTG CAGCTGCTGC AAGATCAGCA 
CAACCGACTG GTAACACGTT CTCAACAACA AATGTGCGGA CAAAATTCCC TTTCCTTCTT 
AAGCATTCTC TACGAGAATA TCAGCACATT GGACTGGACT GGCTTGTTGC TATGTATGAA 
AAGAGGCTGA ATGGAATTCT AGCAGATGAA ATGGGTTTGG GGAAGACAAT TATGACCATC 
TCCTTGCTAG CACACCTTGC ATGTGAGAAG GGAATATGGG GTCCACATCT TATTGTAGTG 
CCAACTAGTG TTATGCTAAA TTGGGAGACT GAATTTCTCA AATGGTGTCC TGCGTTTAAA 
ATACTGACAT ATTTTGGAAG TGCAAAAGAG AGAAAACAGA AACGTCAAGG TTGGATGAAA 
CCAAATTACT TCCATGTATG CATCACTACA TACAGGCTTG TTATTCAGGA CTCCAAAGTG 
TTTAAGCGAA AAAAATGGAA GTATCTTATT CTTGATGAGG CTCATTTGAT AAAGAACTGG 
AAATCACAAA GATGGCAGAC TTTACTAAAT TTTAATTCGA AGCGACGCAT TCTTTTAACT 
GGAACTCCTC TACAAAATGA CCTCATGGAA CTTTGGTCTC TCATGCACTT TTTGATGCCT 
CATGTATTTC AGTCACATCA AGAGTTCAAG GACTGGTTCT GCAATCCAAT TTCAGGCATG 
GTAGAGGGCC AAGACAAGGT AAACAAAGAA GTGATAGATC GGTTGCACAA CGTCCTTCGT 
CCGTTTATAC TTCGTCGATT GAAACGGGAT GTGGAGAAAC AGTTGCCACA GAAGCATGAG 
CATGTCATAT ATTGCCGACT TTCCAGAAGG CAAAGGAACT TGTATGAAGA TTTCATTGCA 
AGCTCAGAGA CACAGGCAAC ATTGGCAAGT GGAAATTATT TTGGCATGAT TAGCATCATT 
ATGCAGCTTA GAAAGGTTTG TAACCATCCA GATCTTTTTG AAGGCCGTCC AATTATCAGC 
TCTTTTGACA TGGCAGGGAT CAACATGCAA ATCAGCTCTT CTGTTGGCAT GGTCCTTGAT 
AAAGGCCCAT TTTCTCAGGT TGACCTGTCA GATATGAATT TTGTATTTAC TCAAAATGAA 
TATAATATGA CTTCTTGGGA GGAAGATGAG GTTGCTGCCA TTTTTTCTCC AAGCATAACC 
CTCAGGGGTT CTGGCATTTC TCGCTCTACT AATGATGGTC AGAGAAGCAA TGGGACAAAT 
ATTTTTGAAG AGATTCACAA TTCTTTGTGG GAGGAGAGAA TAAAAGAAGC TAATGAAAGA 
GCAGCTTCCA TTGCATGGTG GAATAGGGTA AGATGCCAGA AGAGGCCTGT TTATGGCACA 
AATATTCGGG AGGTTTTGAC CATAAAGCAT CCTGTGTCTG ATATTCTCGA GAAGAAGAAG 
AACCCTTTAT GCCACATGGA ATTTTCATCA AGCCTAGCAA ATCTTGTTCT ATCATCGGTG 
GATCGCTTCA AGACAATGCT TGATTTTATT GAGTCATTTA CTTTTGCAAT TCCTGCTGCA 
CGAGCGCCTG CACCATTATT CTGGTGCAAC AAAGGGAAAT CTCCTGTTCT TATTGAACCA 
GCATACAGAG AGAAATGCAT GAATGAGTTT TCTCCTGTTT TCTCTCCTAT AAGGCCTGCG 
ATTGTTCGCC GTCAAGTGTA CTTTCCTGAC AGGCGTTTGA TCCAGTTTGA CTGTGGAAAG 
TTGCAGGAAC TTGCTATTTT GTTGAGGCGT TTGAAGTCAG AAGGACACAG GGCCTTGATA 
TTTACTCAGA TGACTAAGAT GCTTGATATA CTGGAGGAGT TCATTAATCT ATATGGGTAT 
ACGTATTTGC GGTTAGATGG ATCAACCCAG CCAGAAGAGA GGCAGACACT GATGCAGAGG 
TTTAATACTA ACCCAAAGTT TTTTCTTTTT ATCTTATCCA CTCGTAGTGG TGGTGTGGGA 
ATCAACCTAG TAGGTGCAGA CACTGTCATT TTCTATGATA GTGACTGGAA TCCTGCAATG 
GACCAACAGG CCCAAGACAG ATGCCACAGG ATAGGTCAAA CTCGTGAAGT TCACATCTAT 
AGACTGATCA GTGAGAGCAC TATAGAGGAG AATATACTCA AGAAAGCCAA TCAGAAGCGA 
GCTCTTGATG ATCTAGTGAT ACAACGCGGT AGCTACAATA CAGAGTTCTT CAAGAAGCTT 
GATCCTATGG AATTCTTTTC TGGGCACTCA TCACTTCATG CAGAGAATCA GCAGAAGGAT 
TGCTCTCTGT CTGCTGGACC CTCAAACGGA ACTAATCTGG CGCTGTCAAA TGTTGATGTT 
GAAGCAGCTA TTAGACAGGC AGAAGATGAA GCTGACTATA TGGCTCTCAA GAGGCTGGAG 
CAGGAAGAGG CCGTGGACAA TCAAGAGTTC AGCGAGGAGG CTGCTGGAAG ACTTGAGGAG 
GATGATCTTG TAAACGAGGA TGACACTAAA CCTGATGAAC ACACCAATGA AGAGCATAAA 
TATCAGTGTT CTGATTTGGT CAAGGACAAG CATGTTGCTT TATCTATAAA TCAATTAGAT 
GAAGAAAAGG CTATAACTTT GGCTGGAGGA GATGGAGATA TTGACATGCT TGCTGATGTT 
AAGCAGATGG CTGCTGCAGC TGCTGCAGCA GGTCAAGCAA GTTCGTCTTT TGAAAATCAG 
CTCCGGCCAA TTGATAGATA TGCCATGCGG TTTTTGGAAC TCTGGGATCC AATAATTGAT 
AAAGCTGCTA TAAACTATCA GGTCAATGTT GAAGAGGAAG AATGGGAACT TGAACGCATT 
GAAAAACTCA AAGAAGATTT AGAAGCAGAA ATTGATGAAG ACCAGGAACC ACTTTCTTAT 
GAGTCATGGG ATGTTGATTT TGCTACTACA GCATATCGGC AACATGTTGA GGCTCTAGCT 
CAAAAACAGT TGTTTGAGGA ACAAGAAAGA CAAGCCCGTG AAGCAGCAAA AGAGTTGGAG 
GAAAAGAATG ATAATAGCAC TCAACGAAAA AAGTCAAAAA AGAACAAGAA GAAGGCAGCG 
AAGTTCAAAT CTTTGAAGAA AGGACGTTTA TCATCTGAGT CAGAGGTTAT GGTTGAGGAA 
ACATCAGTAG ATACTATGAG CATTGATGAC AATGCACCCT CACCAGAGCT AATGAGCGAT 
GAATCAGCAC ACCATCATTC TAACAAGCAT AAGAGGATAA TGTCAACCAA TGAGGAAGAG 
AACAGCAATA GCAGAAGCCT GAAGAAGCTC AAGAAAGCCC CTAAATCGAG TTTCAGTTCC 
GAGGCCTTAT CACCTAAGCA TTTTCTTGAA GGCAAGCAGC TTAAGTTGAA AGATGAACTT 
AATGATTCTG ATCCCAAATC GGGGGCAAGA ATTAAGAGTG ATGGCCGAAT TTCTGTCCCA 
TGCATGCCAG TAAAGCGTGT CATGGTAATC AAGCCTGAGA GATTGAAGAA GAAGAGCCTA 
TGGTCTCGCG ATTGTGCTTC AGACTCGTGG ACAACTGAGG AAGATGCTGT TCTTTGTGCA 
ACTGTAAATG AGTACGGCCC TCTTTGGGAA CTGGCTAGTG ATTCTCTTCA TTCTGTACCA 
GGTGGTGCAT TTTATAGAGG AAGATACCGT CACCCTGTGC ATTGCTGTGA GAGATTCCGG 
GAATTAGTCT GCAAGCACAT CTTATCAGCA ACAGACAATT CAAACAGTGA GAAGGTTCCT 
TCTGGGACTG GGAAGGCTAT CTTGAAAGTA TCTGAGGATC AAACTCAGAT GTTGCTCAAT 
GTAATCAGCG AGCTTCCTAA CAATGAATTG CTTCTCCAGA AACATTTCAT GGCTGTTCTT 
TCATCTGTTT GGAGATCGAA GTCTGCCTGT GAGTCCCACT GTGTTATGAG TTACTCTAAC 
ACACTCCAGA AACCTGGCAG ACTCAGCGAG AACTGGTCCA TGACAAACTT CAGACCAAAC 
TTTAATCTAG TAAGGACAGC CCTCGCAGAT GCTCAGGTTC AATGTCCAAG AATGGTAGTT 
CCAACAAGCA ATCATGAGTC CCGCCGTAAC TTTTTGGAAT TGGAATTGGA TTTCTTGACA 
GATCGAGATG ATTATGAGGC GGACTTTCCA TCTGTTGTTA ACGTGTCCAT ACTAGAACCG 
GAACCATTAA AACATGCTGT GGAGCCAGTG GAGCACTCAC TACTGTCCAC ACTTTCTTAT 
AGACATGCTG AGAACAGATT CAGGATGGTA TCAGAAACTT GTTTTGAAGG GGAAGGTTCT 
CACTGGGCCT CATCTGCTTT CCACACATAC GATGCCGGTC GGCATAAATC TGGTCCTAAG 
TCCATAGGAA AGCACAAAGC ATCATCAGAA TCTGGAAGGC CTCCCAAATC CAAAATTCAA 
AGGACCACTG AACCACAGGA AGTGCCGGTT ACAAATAATT TTCATAGGAT TCCGGGGCAA 
CTTCTGCATA ACTCAGCAGA GTTCCACATT ACCCAGTCCC TATCTGACCT TGGCATCAGT 
GATTCTGAAT TCACCTACTT TGATAATCTT CCTCAAGAGG CTGAAACAGA GTTTGTTCCG 
TACCAGTATG ACTCTGATGT CCTTTCTGGC ATTGAGGAGT TGGATCCTCT TACAGATTTC 
ACAGACATCG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015625604.1
CDS	404..6538
Translation	MASKGPRSKLDHETRARRQKALEAPRGEPRRTKTHWDHLLGEMAWLAKEFDAERKWKLSMAKRIAQRANKGVVDQATKDERKQKEEEVRLRKVALNISKDVKKFWTKIEKLVFYKNQLELEERKKKALDKQLDFLLGQTERYSTMLAENLVDVRLQNQENDSLQTNQRSQQELAQENINASSPTDVDNVEIDDDYNSSLGEEPKDDEHTIDEDEAQITEAERNEELAALQAEADLPLDDILKLYTKNKVSRESSPDGRDVFSDSDSKDLIKDPLNQANGCNDESDHTSSDEGISSEEADDYQSYSEFVKKNTVKCNGNISSVDAKDDEDYVANDEGKDDEATLSEEEELAKKEDCDPLDEVKLLQKESEIPLEELLARYQKDGYADDDTTELENSPALSVEDVNANMPVDDESADTVEVNRDLSADTMKLTRDQSAETVKVNNDQSAEIVEGNNDTFEDHESAGMLGPEHVSGSVLQLETSEPIVQENTAKEGDVTDTKAMANGDNSDVIADAAAAARSAQPTGNTFSTTNVRTKFPFLLKHSLREYQHIGLDWLVAMYEKRLNGILADEMGLGKTIMTISLLAHLACEKGIWGPHLIVVPTSVMLNWETEFLKWCPAFKILTYFGSAKERKQKRQGWMKPNYFHVCITTYRLVIQDSKVFKRKKWKYLILDEAHLIKNWKSQRWQTLLNFNSKRRILLTGTPLQNDLMELWSLMHFLMPHVFQSHQEFKDWFCNPISGMVEGQDKVNKEVIDRLHNVLRPFILRRLKRDVEKQLPQKHEHVIYCRLSRRQRNLYEDFIASSETQATLASGNYFGMISIIMQLRKVCNHPDLFEGRPIISSFDMAGINMQISSSVGMVLDKGPFSQVDLSDMNFVFTQNEYNMTSWEEDEVAAIFSPSITLRGSGISRSTNDGQRSNGTNIFEEIHNSLWEERIKEANERAASIAWWNRVRCQKRPVYGTNIREVLTIKHPVSDILEKKKNPLCHMEFSSSLANLVLSSVDRFKTMLDFIESFTFAIPAARAPAPLFWCNKGKSPVLIEPAYREKCMNEFSPVFSPIRPAIVRRQVYFPDRRLIQFDCGKLQELAILLRRLKSEGHRALIFTQMTKMLDILEEFINLYGYTYLRLDGSTQPEERQTLMQRFNTNPKFFLFILSTRSGGVGINLVGADTVIFYDSDWNPAMDQQAQDRCHRIGQTREVHIYRLISESTIEENILKKANQKRALDDLVIQRGSYNTEFFKKLDPMEFFSGHSSLHAENQQKDCSLSAGPSNGTNLALSNVDVEAAIRQAEDEADYMALKRLEQEEAVDNQEFSEEAAGRLEEDDLVNEDDTKPDEHTNEEHKYQCSDLVKDKHVALSINQLDEEKAITLAGGDGDIDMLADVKQMAAAAAAAGQASSSFENQLRPIDRYAMRFLELWDPIIDKAAINYQVNVEEEEWELERIEKLKEDLEAEIDEDQEPLSYESWDVDFATTAYRQHVEALAQKQLFEEQERQAREAAKELEEKNDNSTQRKKSKKNKKKAAKFKSLKKGRLSSESEVMVEETSVDTMSIDDNAPSPELMSDESAHHHSNKHKRIMSTNEEENSNSRSLKKLKKAPKSSFSSEALSPKHFLEGKQLKLKDELNDSDPKSGARIKSDGRISVPCMPVKRVMVIKPERLKKKSLWSRDCASDSWTTEEDAVLCATVNEYGPLWELASDSLHSVPGGAFYRGRYRHPVHCCERFRELVCKHILSATDNSNSEKVPSGTGKAILKVSEDQTQMLLNVISELPNNELLLQKHFMAVLSSVWRSKSACESHCVMSYSNTLQKPGRLSENWSMTNFRPNFNLVRTALADAQVQCPRMVVPTSNHESRRNFLELELDFLTDRDDYEADFPSVVNVSILEPEPLKHAVEPVEHSLLSTLSYRHAENRFRMVSETCFEGEGSHWASSAFHTYDAGRHKSGPKSIGKHKASSESGRPPKSKIQRTTEPQEVPVTNNFHRIPGQLLHNSAEFHITQSLSDLGISDSEFTYFDNLPQEAETEFVPYQYDSDVLSGIEELDPLTDFTDIG

Target ORF information:

RefSeq Version	XM_015770118.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015770118.2

ORF Insert Sequence:

ATGGCATCAA AAGGTCCTCG ATCAAAGTTA GATCATGAGA CAAGAGCCAG ACGCCAGAAG 
GCTCTTGAAG CCCCAAGGGG GGAGCCTCGA AGGACGAAAA CTCACTGGGA CCATCTTCTT 
GGAGAAATGG CTTGGCTGGC AAAGGAGTTT GATGCTGAGA GAAAATGGAA GCTGTCCATG 
GCTAAAAGGA TTGCTCAAAG AGCTAATAAG GGTGTAGTTG ACCAAGCAAC AAAGGACGAG 
AGAAAACAGA AGGAGGAAGA AGTTCGCCTG AGAAAAGTTG CCCTAAATAT TTCTAAGGAC 
GTGAAGAAGT TCTGGACCAA AATAGAGAAG CTGGTTTTTT ATAAGAACCA ACTGGAGCTT 
GAGGAAAGGA AAAAAAAGGC CCTAGACAAG CAACTTGATT TCCTCTTAGG ACAGACTGAA 
AGATATTCAA CAATGCTGGC AGAAAATCTT GTTGATGTGC GCCTTCAAAA TCAAGAAAAT 
GACTCTTTAC AGACTAATCA AAGATCCCAG CAGGAACTAG CACAAGAGAA CATAAATGCA 
TCAAGTCCGA CTGATGTTGA CAATGTGGAA ATCGATGATG ATTACAACAG TTCCTTGGGT 
GAAGAGCCAA AAGATGATGA ACACACAATC GATGAGGATG AAGCTCAAAT TACGGAGGCT 
GAGCGCAACG AGGAATTGGC TGCTTTGCAG GCAGAAGCTG ATCTACCACT GGATGATATT 
CTCAAGTTGT ACACTAAAAA TAAAGTTAGC AGGGAAAGCA GTCCAGATGG CAGAGACGTT 
TTTAGTGACT CAGATTCAAA GGATTTGATT AAAGATCCTT TGAACCAGGC TAATGGATGC 
AACGATGAAT CTGATCATAC TTCAAGTGAT GAAGGCATTT CTTCTGAGGA AGCAGATGAT 
TACCAGTCTT ACTCTGAGTT TGTGAAAAAG AACACTGTGA AATGCAATGG CAATATCTCA 
TCTGTTGATG CAAAGGACGA CGAAGATTAT GTTGCTAATG ATGAAGGAAA GGATGATGAA 
GCTACTTTGT CTGAAGAGGA AGAGTTGGCG AAGAAAGAAG ACTGTGATCC TCTAGATGAG 
GTTAAGTTAC TGCAGAAAGA GAGTGAGATC CCACTTGAAG AACTTCTTGC AAGATACCAA 
AAGGATGGCT ATGCAGATGA TGACACAACA GAATTGGAAA ATTCACCTGC ACTATCAGTT 
GAGGATGTCA ATGCCAACAT GCCTGTGGAT GATGAGTCTG CAGACACCGT GGAGGTAAAC 
AGGGATCTTT CTGCAGATAC CATGAAACTG ACCAGAGATC AGTCTGCAGA GACCGTAAAA 
GTGAATAATG ACCAGTCTGC AGAGATTGTG GAGGGCAACA ATGACACATT TGAAGATCAT 
GAATCTGCAG GCATGCTGGG ACCTGAGCAT GTCAGTGGTA GTGTTCTACA ACTAGAAACA 
TCAGAGCCTA TTGTACAGGA AAATACTGCT AAAGAGGGTG ATGTAACTGA TACTAAAGCG 
ATGGCCAATG GAGATAATAG TGATGTGATT GCTGATGCTG CAGCTGCTGC AAGATCAGCA 
CAACCGACTG GTAACACGTT CTCAACAACA AATGTGCGGA CAAAATTCCC TTTCCTTCTT 
AAGCATTCTC TACGAGAATA TCAGCACATT GGACTGGACT GGCTTGTTGC TATGTATGAA 
AAGAGGCTGA ATGGAATTCT AGCAGATGAA ATGGGTTTGG GGAAGACAAT TATGACCATC 
TCCTTGCTAG CACACCTTGC ATGTGAGAAG GGAATATGGG GTCCACATCT TATTGTAGTG 
CCAACTAGTG TTATGCTAAA TTGGGAGACT GAATTTCTCA AATGGTGTCC TGCGTTTAAA 
ATACTGACAT ATTTTGGAAG TGCAAAAGAG AGAAAACAGA AACGTCAAGG TTGGATGAAA 
CCAAATTACT TCCATGTATG CATCACTACA TACAGGCTTG TTATTCAGGA CTCCAAAGTG 
TTTAAGCGAA AAAAATGGAA GTATCTTATT CTTGATGAGG CTCATTTGAT AAAGAACTGG 
AAATCACAAA GATGGCAGAC TTTACTAAAT TTTAATTCGA AGCGACGCAT TCTTTTAACT 
GGAACTCCTC TACAAAATGA CCTCATGGAA CTTTGGTCTC TCATGCACTT TTTGATGCCT 
CATGTATTTC AGTCACATCA AGAGTTCAAG GACTGGTTCT GCAATCCAAT TTCAGGCATG 
GTAGAGGGCC AAGACAAGGT AAACAAAGAA GTGATAGATC GGTTGCACAA CGTCCTTCGT 
CCGTTTATAC TTCGTCGATT GAAACGGGAT GTGGAGAAAC AGTTGCCACA GAAGCATGAG 
CATGTCATAT ATTGCCGACT TTCCAGAAGG CAAAGGAACT TGTATGAAGA TTTCATTGCA 
AGCTCAGAGA CACAGGCAAC ATTGGCAAGT GGAAATTATT TTGGCATGAT TAGCATCATT 
ATGCAGCTTA GAAAGGTTTG TAACCATCCA GATCTTTTTG AAGGCCGTCC AATTATCAGC 
TCTTTTGACA TGGCAGGGAT CAACATGCAA ATCAGCTCTT CTGTTGGCAT GGTCCTTGAT 
AAAGGCCCAT TTTCTCAGGT TGACCTGTCA GATATGAATT TTGTATTTAC TCAAAATGAA 
TATAATATGA CTTCTTGGGA GGAAGATGAG GTTGCTGCCA TTTTTTCTCC AAGCATAACC 
CTCAGGGGTT CTGGCATTTC TCGCTCTACT AATGATGGTC AGAGAAGCAA TGGGACAAAT 
ATTTTTGAAG AGATTCACAA TTCTTTGTGG GAGGAGAGAA TAAAAGAAGC TAATGAAAGA 
GCAGCTTCCA TTGCATGGTG GAATAGGGTA AGATGCCAGA AGAGGCCTGT TTATGGCACA 
AATATTCGGG AGGTTTTGAC CATAAAGCAT CCTGTGTCTG ATATTCTCGA GAAGAAGAAG 
AACCCTTTAT GCCACATGGA ATTTTCATCA AGCCTAGCAA ATCTTGTTCT ATCATCGGTG 
GATCGCTTCA AGACAATGCT TGATTTTATT GAGTCATTTA CTTTTGCAAT TCCTGCTGCA 
CGAGCGCCTG CACCATTATT CTGGTGCAAC AAAGGGAAAT CTCCTGTTCT TATTGAACCA 
GCATACAGAG AGAAATGCAT GAATGAGTTT TCTCCTGTTT TCTCTCCTAT AAGGCCTGCG 
ATTGTTCGCC GTCAAGTGTA CTTTCCTGAC AGGCGTTTGA TCCAGTTTGA CTGTGGAAAG 
TTGCAGGAAC TTGCTATTTT GTTGAGGCGT TTGAAGTCAG AAGGACACAG GGCCTTGATA 
TTTACTCAGA TGACTAAGAT GCTTGATATA CTGGAGGAGT TCATTAATCT ATATGGGTAT 
ACGTATTTGC GGTTAGATGG ATCAACCCAG CCAGAAGAGA GGCAGACACT GATGCAGAGG 
TTTAATACTA ACCCAAAGTT TTTTCTTTTT ATCTTATCCA CTCGTAGTGG TGGTGTGGGA 
ATCAACCTAG TAGGTGCAGA CACTGTCATT TTCTATGATA GTGACTGGAA TCCTGCAATG 
GACCAACAGG CCCAAGACAG ATGCCACAGG ATAGGTCAAA CTCGTGAAGT TCACATCTAT 
AGACTGATCA GTGAGAGCAC TATAGAGGAG AATATACTCA AGAAAGCCAA TCAGAAGCGA 
GCTCTTGATG ATCTAGTGAT ACAACGCGGT AGCTACAATA CAGAGTTCTT CAAGAAGCTT 
GATCCTATGG AATTCTTTTC TGGGCACTCA TCACTTCATG CAGAGAATCA GCAGAAGGAT 
TGCTCTCTGT CTGCTGGACC CTCAAACGGA ACTAATCTGG CGCTGTCAAA TGTTGATGTT 
GAAGCAGCTA TTAGACAGGC AGAAGATGAA GCTGACTATA TGGCTCTCAA GAGGCTGGAG 
CAGGAAGAGG CCGTGGACAA TCAAGAGTTC AGCGAGGAGG CTGCTGGAAG ACTTGAGGAG 
GATGATCTTG TAAACGAGGA TGACACTAAA CCTGATGAAC ACACCAATGA AGAGCATAAA 
TATCAGTGTT CTGATTTGGT CAAGGACAAG CATGTTGCTT TATCTATAAA TCAATTAGAT 
GAAGAAAAGG CTATAACTTT GGCTGGAGGA GATGGAGATA TTGACATGCT TGCTGATGTT 
AAGCAGATGG CTGCTGCAGC TGCTGCAGCA GGTCAAGCAA GTTCGTCTTT TGAAAATCAG 
CTCCGGCCAA TTGATAGATA TGCCATGCGG TTTTTGGAAC TCTGGGATCC AATAATTGAT 
AAAGCTGCTA TAAACTATCA GGTCAATGTT GAAGAGGAAG AATGGGAACT TGAACGCATT 
GAAAAACTCA AAGAAGATTT AGAAGCAGAA ATTGATGAAG ACCAGGAACC ACTTTCTTAT 
GAGTCATGGG ATGTTGATTT TGCTACTACA GCATATCGGC AACATGTTGA GGCTCTAGCT 
CAAAAACAGT TGTTTGAGGA ACAAGAAAGA CAAGCCCGTG AAGCAGCAAA AGAGTTGGAG 
GAAAAGAATG ATAATAGCAC TCAACGAAAA AAGTCAAAAA AGAACAAGAA GAAGGCAGCG 
AAGTTCAAAT CTTTGAAGAA AGGACGTTTA TCATCTGAGT CAGAGGTTAT GGTTGAGGAA 
ACATCAGTAG ATACTATGAG CATTGATGAC AATGCACCCT CACCAGAGCT AATGAGCGAT 
GAATCAGCAC ACCATCATTC TAACAAGCAT AAGAGGATAA TGTCAACCAA TGAGGAAGAG 
AACAGCAATA GCAGAAGCCT GAAGAAGCTC AAGAAAGCCC CTAAATCGAG TTTCAGTTCC 
GAGGCCTTAT CACCTAAGCA TTTTCTTGAA GGCAAGCAGC TTAAGTTGAA AGATGAACTT 
AATGATTCTG ATCCCAAATC GGGGGCAAGA ATTAAGAGTG ATGGCCGAAT TTCTGTCCCA 
TGCATGCCAG TAAAGCGTGT CATGGTAATC AAGCCTGAGA GATTGAAGAA GAAGAGCCTA 
TGGTCTCGCG ATTGTGCTTC AGACTCGTGG ACAACTGAGG AAGATGCTGT TCTTTGTGCA 
ACTGTAAATG AGTACGGCCC TCTTTGGGAA CTGGCTAGTG ATTCTCTTCA TTCTGTACCA 
GGTGGTGCAT TTTATAGAGG AAGATACCGT CACCCTGTGC ATTGCTGTGA GAGATTCCGG 
GAATTAGTCT GCAAGCACAT CTTATCAGCA ACAGACAATT CAAACAGTGA GAAGGTTCCT 
TCTGGGACTG GGAAGGCTAT CTTGAAAGTA TCTGAGGATC AAACTCAGAT GTTGCTCAAT 
GTAATCAGCG AGCTTCCTAA CAATGAATTG CTTCTCCAGA AACATTTCAT GGCTGTTCTT 
TCATCTGTTT GGAGATCGAA GTCTGCCTGT GAGTCCCACT GTGTTATGAG TTACTCTAAC 
ACACTCCAGA AACCTGGCAG ACTCAGCGAG AACTGGTCCA TGACAAACTT CAGACCAAAC 
TTTAATCTAG TAAGGACAGC CCTCGCAGAT GCTCAGGTTC AATGTCCAAG AATGGTAGTT 
CCAACAAGCA ATCATGAGTC CCGCCGTAAC TTTTTGGAAT TGGAATTGGA TTTCTTGACA 
GATCGAGATG ATTATGAGGC GGACTTTCCA TCTGTTGTTA ACGTGTCCAT ACTAGAACCG 
GAACCATTAA AACATGCTGT GGAGCCAGTG GAGCACTCAC TACTGTCCAC ACTTTCTTAT 
AGACATGCTG AGAACAGATT CAGGATGGTA TCAGAAACTT GTTTTGAAGG GGAAGGTTCT 
CACTGGGCCT CATCTGCTTT CCACACATAC GATGCCGGTC GGCATAAATC TGGTCCTAAG 
TCCATAGGAA AGCACAAAGC ATCATCAGAA TCTGGAAGGC CTCCCAAATC CAAAATTCAA 
AGGACCACTG AACCACAGGA AGTGCCGGTT ACAAATAATT TTCATAGGAT TCCGGGGCAA 
CTTCTGCATA ACTCAGCAGA GTTCCACATT ACCCAGTCCC TATCTGACCT TGGCATCAGT 
GATTCTGAAT TCACCTACTT TGATAATCTT CCTCAAGAGG CTGAAACAGA GTTTGTTCCG 
TACCAGTATG ACTCTGATGT CCTTTCTGGC ATTGAGGAGT TGGATCCTCT TACAGATTTC 
ACAGACATCG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4330371 ( XM_015770119.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015625605.1 Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X2, mRNA.

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CloneID	OOa28159
Clone ID Related Accession (Same CDS sequence)	XM_015770118.2 , XM_015770119.1 , XM_015770118.1
Accession Version	XM_015770119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379175.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCAA AAGGTCCTCG ATCAAAGTTA GATCATGAGA CAAGAGCCAG ACGCCAGAAG 
GCTCTTGAAG CCCCAAGGGG GGAGCCTCGA AGGACGAAAA CTCACTGGGA CCATCTTCTT 
GGAGAAATGG CTTGGCTGGC AAAGGAGTTT GATGCTGAGA GAAAATGGAA GCTGTCCATG 
GCTAAAAGGA TTGCTCAAAG AGCTAATAAG GGTGTAGTTG ACCAAGCAAC AAAGGACGAG 
AGAAAACAGA AGGAGGAAGA AGTTCGCCTG AGAAAAGTTG CCCTAAATAT TTCTAAGGAC 
GTGAAGAAGT TCTGGACCAA AATAGAGAAG CTGGTTTTTT ATAAGAACCA ACTGGAGCTT 
GAGGAAAGGA AAAAAAAGGC CCTAGACAAG CAACTTGATT TCCTCTTAGG ACAGACTGAA 
AGATATTCAA CAATGCTGGC AGAAAATCTT GTTGATGTGC GCCTTCAAAA TCAAGAAAAT 
GACTCTTTAC AGACTAATCA AAGATCCCAG CAGGAACTAG CACAAGAGAA CATAAATGCA 
TCAAGTCCGA CTGATGTTGA CAATGTGGAA ATCGATGATG ATTACAACAG TTCCTTGGGT 
GAAGAGCCAA AAGATGATGA ACACACAATC GATGAGGATG AAGCTCAAAT TACGGAGGCT 
GAGCGCAACG AGGAATTGGC TGCTTTGCAG GCAGAAGCTG ATCTACCACT GGATGATATT 
CTCAAGTTGT ACACTAAAAA TAAAGTTAGC AGGGAAAGCA GTCCAGATGG CAGAGACGTT 
TTTAGTGACT CAGATTCAAA GGATTTGATT AAAGATCCTT TGAACCAGGC TAATGGATGC 
AACGATGAAT CTGATCATAC TTCAAGTGAT GAAGGCATTT CTTCTGAGGA AGCAGATGAT 
TACCAGTCTT ACTCTGAGTT TGTGAAAAAG AACACTGTGA AATGCAATGG CAATATCTCA 
TCTGTTGATG CAAAGGACGA CGAAGATTAT GTTGCTAATG ATGAAGGAAA GGATGATGAA 
GCTACTTTGT CTGAAGAGGA AGAGTTGGCG AAGAAAGAAG ACTGTGATCC TCTAGATGAG 
GTTAAGTTAC TGCAGAAAGA GAGTGAGATC CCACTTGAAG AACTTCTTGC AAGATACCAA 
AAGGATGGCT ATGCAGATGA TGACACAACA GAATTGGAAA ATTCACCTGC ACTATCAGTT 
GAGGATGTCA ATGCCAACAT GCCTGTGGAT GATGAGTCTG CAGACACCGT GGAGGTAAAC 
AGGGATCTTT CTGCAGATAC CATGAAACTG ACCAGAGATC AGTCTGCAGA GACCGTAAAA 
GTGAATAATG ACCAGTCTGC AGAGATTGTG GAGGGCAACA ATGACACATT TGAAGATCAT 
GAATCTGCAG GCATGCTGGG ACCTGAGCAT GTCAGTGGTA GTGTTCTACA ACTAGAAACA 
TCAGAGCCTA TTGTACAGGA AAATACTGCT AAAGAGGGTG ATGTAACTGA TACTAAAGCG 
ATGGCCAATG GAGATAATAG TGATGTGATT GCTGATGCTG CAGCTGCTGC AAGATCAGCA 
CAACCGACTG GTAACACGTT CTCAACAACA AATGTGCGGA CAAAATTCCC TTTCCTTCTT 
AAGCATTCTC TACGAGAATA TCAGCACATT GGACTGGACT GGCTTGTTGC TATGTATGAA 
AAGAGGCTGA ATGGAATTCT AGCAGATGAA ATGGGTTTGG GGAAGACAAT TATGACCATC 
TCCTTGCTAG CACACCTTGC ATGTGAGAAG GGAATATGGG GTCCACATCT TATTGTAGTG 
CCAACTAGTG TTATGCTAAA TTGGGAGACT GAATTTCTCA AATGGTGTCC TGCGTTTAAA 
ATACTGACAT ATTTTGGAAG TGCAAAAGAG AGAAAACAGA AACGTCAAGG TTGGATGAAA 
CCAAATTACT TCCATGTATG CATCACTACA TACAGGCTTG TTATTCAGGA CTCCAAAGTG 
TTTAAGCGAA AAAAATGGAA GTATCTTATT CTTGATGAGG CTCATTTGAT AAAGAACTGG 
AAATCACAAA GATGGCAGAC TTTACTAAAT TTTAATTCGA AGCGACGCAT TCTTTTAACT 
GGAACTCCTC TACAAAATGA CCTCATGGAA CTTTGGTCTC TCATGCACTT TTTGATGCCT 
CATGTATTTC AGTCACATCA AGAGTTCAAG GACTGGTTCT GCAATCCAAT TTCAGGCATG 
GTAGAGGGCC AAGACAAGGT AAACAAAGAA GTGATAGATC GGTTGCACAA CGTCCTTCGT 
CCGTTTATAC TTCGTCGATT GAAACGGGAT GTGGAGAAAC AGTTGCCACA GAAGCATGAG 
CATGTCATAT ATTGCCGACT TTCCAGAAGG CAAAGGAACT TGTATGAAGA TTTCATTGCA 
AGCTCAGAGA CACAGGCAAC ATTGGCAAGT GGAAATTATT TTGGCATGAT TAGCATCATT 
ATGCAGCTTA GAAAGGTTTG TAACCATCCA GATCTTTTTG AAGGCCGTCC AATTATCAGC 
TCTTTTGACA TGGCAGGGAT CAACATGCAA ATCAGCTCTT CTGTTGGCAT GGTCCTTGAT 
AAAGGCCCAT TTTCTCAGGT TGACCTGTCA GATATGAATT TTGTATTTAC TCAAAATGAA 
TATAATATGA CTTCTTGGGA GGAAGATGAG GTTGCTGCCA TTTTTTCTCC AAGCATAACC 
CTCAGGGGTT CTGGCATTTC TCGCTCTACT AATGATGGTC AGAGAAGCAA TGGGACAAAT 
ATTTTTGAAG AGATTCACAA TTCTTTGTGG GAGGAGAGAA TAAAAGAAGC TAATGAAAGA 
GCAGCTTCCA TTGCATGGTG GAATAGGGTA AGATGCCAGA AGAGGCCTGT TTATGGCACA 
AATATTCGGG AGGTTTTGAC CATAAAGCAT CCTGTGTCTG ATATTCTCGA GAAGAAGAAG 
AACCCTTTAT GCCACATGGA ATTTTCATCA AGCCTAGCAA ATCTTGTTCT ATCATCGGTG 
GATCGCTTCA AGACAATGCT TGATTTTATT GAGTCATTTA CTTTTGCAAT TCCTGCTGCA 
CGAGCGCCTG CACCATTATT CTGGTGCAAC AAAGGGAAAT CTCCTGTTCT TATTGAACCA 
GCATACAGAG AGAAATGCAT GAATGAGTTT TCTCCTGTTT TCTCTCCTAT AAGGCCTGCG 
ATTGTTCGCC GTCAAGTGTA CTTTCCTGAC AGGCGTTTGA TCCAGTTTGA CTGTGGAAAG 
TTGCAGGAAC TTGCTATTTT GTTGAGGCGT TTGAAGTCAG AAGGACACAG GGCCTTGATA 
TTTACTCAGA TGACTAAGAT GCTTGATATA CTGGAGGAGT TCATTAATCT ATATGGGTAT 
ACGTATTTGC GGTTAGATGG ATCAACCCAG CCAGAAGAGA GGCAGACACT GATGCAGAGG 
TTTAATACTA ACCCAAAGTT TTTTCTTTTT ATCTTATCCA CTCGTAGTGG TGGTGTGGGA 
ATCAACCTAG TAGGTGCAGA CACTGTCATT TTCTATGATA GTGACTGGAA TCCTGCAATG 
GACCAACAGG CCCAAGACAG ATGCCACAGG ATAGGTCAAA CTCGTGAAGT TCACATCTAT 
AGACTGATCA GTGAGAGCAC TATAGAGGAG AATATACTCA AGAAAGCCAA TCAGAAGCGA 
GCTCTTGATG ATCTAGTGAT ACAACGCGGT AGCTACAATA CAGAGTTCTT CAAGAAGCTT 
GATCCTATGG AATTCTTTTC TGGGCACTCA TCACTTCATG CAGAGAATCA GCAGAAGGAT 
TGCTCTCTGT CTGCTGGACC CTCAAACGGA ACTAATCTGG CGCTGTCAAA TGTTGATGTT 
GAAGCAGCTA TTAGACAGGC AGAAGATGAA GCTGACTATA TGGCTCTCAA GAGGCTGGAG 
CAGGAAGAGG CCGTGGACAA TCAAGAGTTC AGCGAGGAGG CTGCTGGAAG ACTTGAGGAG 
GATGATCTTG TAAACGAGGA TGACACTAAA CCTGATGAAC ACACCAATGA AGAGCATAAA 
TATCAGTGTT CTGATTTGGT CAAGGACAAG CATGTTGCTT TATCTATAAA TCAATTAGAT 
GAAGAAAAGG CTATAACTTT GGCTGGAGGA GATGGAGATA TTGACATGCT TGCTGATGTT 
AAGCAGATGG CTGCTGCAGC TGCTGCAGCA GGTCAAGCAA GTTCGTCTTT TGAAAATCAG 
CTCCGGCCAA TTGATAGATA TGCCATGCGG TTTTTGGAAC TCTGGGATCC AATAATTGAT 
AAAGCTGCTA TAAACTATCA GGTCAATGTT GAAGAGGAAG AATGGGAACT TGAACGCATT 
GAAAAACTCA AAGAAGATTT AGAAGCAGAA ATTGATGAAG ACCAGGAACC ACTTTCTTAT 
GAGTCATGGG ATGTTGATTT TGCTACTACA GCATATCGGC AACATGTTGA GGCTCTAGCT 
CAAAAACAGT TGTTTGAGGA ACAAGAAAGA CAAGCCCGTG AAGCAGCAAA AGAGTTGGAG 
GAAAAGAATG ATAATAGCAC TCAACGAAAA AAGTCAAAAA AGAACAAGAA GAAGGCAGCG 
AAGTTCAAAT CTTTGAAGAA AGGACGTTTA TCATCTGAGT CAGAGGTTAT GGTTGAGGAA 
ACATCAGTAG ATACTATGAG CATTGATGAC AATGCACCCT CACCAGAGCT AATGAGCGAT 
GAATCAGCAC ACCATCATTC TAACAAGCAT AAGAGGATAA TGTCAACCAA TGAGGAAGAG 
AACAGCAATA GCAGAAGCCT GAAGAAGCTC AAGAAAGCCC CTAAATCGAG TTTCAGTTCC 
GAGGCCTTAT CACCTAAGCA TTTTCTTGAA GGCAAGCAGC TTAAGTTGAA AGATGAACTT 
AATGATTCTG ATCCCAAATC GGGGGCAAGA ATTAAGAGTG ATGGCCGAAT TTCTGTCCCA 
TGCATGCCAG TAAAGCGTGT CATGGTAATC AAGCCTGAGA GATTGAAGAA GAAGAGCCTA 
TGGTCTCGCG ATTGTGCTTC AGACTCGTGG ACAACTGAGG AAGATGCTGT TCTTTGTGCA 
ACTGTAAATG AGTACGGCCC TCTTTGGGAA CTGGCTAGTG ATTCTCTTCA TTCTGTACCA 
GGTGGTGCAT TTTATAGAGG AAGATACCGT CACCCTGTGC ATTGCTGTGA GAGATTCCGG 
GAATTAGTCT GCAAGCACAT CTTATCAGCA ACAGACAATT CAAACAGTGA GAAGGTTCCT 
TCTGGGACTG GGAAGGCTAT CTTGAAAGTA TCTGAGGATC AAACTCAGAT GTTGCTCAAT 
GTAATCAGCG AGCTTCCTAA CAATGAATTG CTTCTCCAGA AACATTTCAT GGCTGTTCTT 
TCATCTGTTT GGAGATCGAA GTCTGCCTGT GAGTCCCACT GTGTTATGAG TTACTCTAAC 
ACACTCCAGA AACCTGGCAG ACTCAGCGAG AACTGGTCCA TGACAAACTT CAGACCAAAC 
TTTAATCTAG TAAGGACAGC CCTCGCAGAT GCTCAGGTTC AATGTCCAAG AATGGTAGTT 
CCAACAAGCA ATCATGAGTC CCGCCGTAAC TTTTTGGAAT TGGAATTGGA TTTCTTGACA 
GATCGAGATG ATTATGAGGC GGACTTTCCA TCTGTTGTTA ACGTGTCCAT ACTAGAACCG 
GAACCATTAA AACATGCTGT GGAGCCAGTG GAGCACTCAC TACTGTCCAC ACTTTCTTAT 
AGACATGCTG AGAACAGATT CAGGATGGTA TCAGAAACTT GTTTTGAAGG GGAAGGTTCT 
CACTGGGCCT CATCTGCTTT CCACACATAC GATGCCGGTC GGCATAAATC TGGTCCTAAG 
TCCATAGGAA AGCACAAAGC ATCATCAGAA TCTGGAAGGC CTCCCAAATC CAAAATTCAA 
AGGACCACTG AACCACAGGA AGTGCCGGTT ACAAATAATT TTCATAGGAT TCCGGGGCAA 
CTTCTGCATA ACTCAGCAGA GTTCCACATT ACCCAGTCCC TATCTGACCT TGGCATCAGT 
GATTCTGAAT TCACCTACTT TGATAATCTT CCTCAAGAGG CTGAAACAGA GTTTGTTCCG 
TACCAGTATG ACTCTGATGT CCTTTCTGGC ATTGAGGAGT TGGATCCTCT TACAGATTTC 
ACAGACATCG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015625605.1
CDS	214..6348
Translation	MASKGPRSKLDHETRARRQKALEAPRGEPRRTKTHWDHLLGEMAWLAKEFDAERKWKLSMAKRIAQRANKGVVDQATKDERKQKEEEVRLRKVALNISKDVKKFWTKIEKLVFYKNQLELEERKKKALDKQLDFLLGQTERYSTMLAENLVDVRLQNQENDSLQTNQRSQQELAQENINASSPTDVDNVEIDDDYNSSLGEEPKDDEHTIDEDEAQITEAERNEELAALQAEADLPLDDILKLYTKNKVSRESSPDGRDVFSDSDSKDLIKDPLNQANGCNDESDHTSSDEGISSEEADDYQSYSEFVKKNTVKCNGNISSVDAKDDEDYVANDEGKDDEATLSEEEELAKKEDCDPLDEVKLLQKESEIPLEELLARYQKDGYADDDTTELENSPALSVEDVNANMPVDDESADTVEVNRDLSADTMKLTRDQSAETVKVNNDQSAEIVEGNNDTFEDHESAGMLGPEHVSGSVLQLETSEPIVQENTAKEGDVTDTKAMANGDNSDVIADAAAAARSAQPTGNTFSTTNVRTKFPFLLKHSLREYQHIGLDWLVAMYEKRLNGILADEMGLGKTIMTISLLAHLACEKGIWGPHLIVVPTSVMLNWETEFLKWCPAFKILTYFGSAKERKQKRQGWMKPNYFHVCITTYRLVIQDSKVFKRKKWKYLILDEAHLIKNWKSQRWQTLLNFNSKRRILLTGTPLQNDLMELWSLMHFLMPHVFQSHQEFKDWFCNPISGMVEGQDKVNKEVIDRLHNVLRPFILRRLKRDVEKQLPQKHEHVIYCRLSRRQRNLYEDFIASSETQATLASGNYFGMISIIMQLRKVCNHPDLFEGRPIISSFDMAGINMQISSSVGMVLDKGPFSQVDLSDMNFVFTQNEYNMTSWEEDEVAAIFSPSITLRGSGISRSTNDGQRSNGTNIFEEIHNSLWEERIKEANERAASIAWWNRVRCQKRPVYGTNIREVLTIKHPVSDILEKKKNPLCHMEFSSSLANLVLSSVDRFKTMLDFIESFTFAIPAARAPAPLFWCNKGKSPVLIEPAYREKCMNEFSPVFSPIRPAIVRRQVYFPDRRLIQFDCGKLQELAILLRRLKSEGHRALIFTQMTKMLDILEEFINLYGYTYLRLDGSTQPEERQTLMQRFNTNPKFFLFILSTRSGGVGINLVGADTVIFYDSDWNPAMDQQAQDRCHRIGQTREVHIYRLISESTIEENILKKANQKRALDDLVIQRGSYNTEFFKKLDPMEFFSGHSSLHAENQQKDCSLSAGPSNGTNLALSNVDVEAAIRQAEDEADYMALKRLEQEEAVDNQEFSEEAAGRLEEDDLVNEDDTKPDEHTNEEHKYQCSDLVKDKHVALSINQLDEEKAITLAGGDGDIDMLADVKQMAAAAAAAGQASSSFENQLRPIDRYAMRFLELWDPIIDKAAINYQVNVEEEEWELERIEKLKEDLEAEIDEDQEPLSYESWDVDFATTAYRQHVEALAQKQLFEEQERQAREAAKELEEKNDNSTQRKKSKKNKKKAAKFKSLKKGRLSSESEVMVEETSVDTMSIDDNAPSPELMSDESAHHHSNKHKRIMSTNEEENSNSRSLKKLKKAPKSSFSSEALSPKHFLEGKQLKLKDELNDSDPKSGARIKSDGRISVPCMPVKRVMVIKPERLKKKSLWSRDCASDSWTTEEDAVLCATVNEYGPLWELASDSLHSVPGGAFYRGRYRHPVHCCERFRELVCKHILSATDNSNSEKVPSGTGKAILKVSEDQTQMLLNVISELPNNELLLQKHFMAVLSSVWRSKSACESHCVMSYSNTLQKPGRLSENWSMTNFRPNFNLVRTALADAQVQCPRMVVPTSNHESRRNFLELELDFLTDRDDYEADFPSVVNVSILEPEPLKHAVEPVEHSLLSTLSYRHAENRFRMVSETCFEGEGSHWASSAFHTYDAGRHKSGPKSIGKHKASSESGRPPKSKIQRTTEPQEVPVTNNFHRIPGQLLHNSAEFHITQSLSDLGISDSEFTYFDNLPQEAETEFVPYQYDSDVLSGIEELDPLTDFTDIG

Target ORF information:

RefSeq Version	XM_015770119.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015770119.1

ORF Insert Sequence:

ATGGCATCAA AAGGTCCTCG ATCAAAGTTA GATCATGAGA CAAGAGCCAG ACGCCAGAAG 
GCTCTTGAAG CCCCAAGGGG GGAGCCTCGA AGGACGAAAA CTCACTGGGA CCATCTTCTT 
GGAGAAATGG CTTGGCTGGC AAAGGAGTTT GATGCTGAGA GAAAATGGAA GCTGTCCATG 
GCTAAAAGGA TTGCTCAAAG AGCTAATAAG GGTGTAGTTG ACCAAGCAAC AAAGGACGAG 
AGAAAACAGA AGGAGGAAGA AGTTCGCCTG AGAAAAGTTG CCCTAAATAT TTCTAAGGAC 
GTGAAGAAGT TCTGGACCAA AATAGAGAAG CTGGTTTTTT ATAAGAACCA ACTGGAGCTT 
GAGGAAAGGA AAAAAAAGGC CCTAGACAAG CAACTTGATT TCCTCTTAGG ACAGACTGAA 
AGATATTCAA CAATGCTGGC AGAAAATCTT GTTGATGTGC GCCTTCAAAA TCAAGAAAAT 
GACTCTTTAC AGACTAATCA AAGATCCCAG CAGGAACTAG CACAAGAGAA CATAAATGCA 
TCAAGTCCGA CTGATGTTGA CAATGTGGAA ATCGATGATG ATTACAACAG TTCCTTGGGT 
GAAGAGCCAA AAGATGATGA ACACACAATC GATGAGGATG AAGCTCAAAT TACGGAGGCT 
GAGCGCAACG AGGAATTGGC TGCTTTGCAG GCAGAAGCTG ATCTACCACT GGATGATATT 
CTCAAGTTGT ACACTAAAAA TAAAGTTAGC AGGGAAAGCA GTCCAGATGG CAGAGACGTT 
TTTAGTGACT CAGATTCAAA GGATTTGATT AAAGATCCTT TGAACCAGGC TAATGGATGC 
AACGATGAAT CTGATCATAC TTCAAGTGAT GAAGGCATTT CTTCTGAGGA AGCAGATGAT 
TACCAGTCTT ACTCTGAGTT TGTGAAAAAG AACACTGTGA AATGCAATGG CAATATCTCA 
TCTGTTGATG CAAAGGACGA CGAAGATTAT GTTGCTAATG ATGAAGGAAA GGATGATGAA 
GCTACTTTGT CTGAAGAGGA AGAGTTGGCG AAGAAAGAAG ACTGTGATCC TCTAGATGAG 
GTTAAGTTAC TGCAGAAAGA GAGTGAGATC CCACTTGAAG AACTTCTTGC AAGATACCAA 
AAGGATGGCT ATGCAGATGA TGACACAACA GAATTGGAAA ATTCACCTGC ACTATCAGTT 
GAGGATGTCA ATGCCAACAT GCCTGTGGAT GATGAGTCTG CAGACACCGT GGAGGTAAAC 
AGGGATCTTT CTGCAGATAC CATGAAACTG ACCAGAGATC AGTCTGCAGA GACCGTAAAA 
GTGAATAATG ACCAGTCTGC AGAGATTGTG GAGGGCAACA ATGACACATT TGAAGATCAT 
GAATCTGCAG GCATGCTGGG ACCTGAGCAT GTCAGTGGTA GTGTTCTACA ACTAGAAACA 
TCAGAGCCTA TTGTACAGGA AAATACTGCT AAAGAGGGTG ATGTAACTGA TACTAAAGCG 
ATGGCCAATG GAGATAATAG TGATGTGATT GCTGATGCTG CAGCTGCTGC AAGATCAGCA 
CAACCGACTG GTAACACGTT CTCAACAACA AATGTGCGGA CAAAATTCCC TTTCCTTCTT 
AAGCATTCTC TACGAGAATA TCAGCACATT GGACTGGACT GGCTTGTTGC TATGTATGAA 
AAGAGGCTGA ATGGAATTCT AGCAGATGAA ATGGGTTTGG GGAAGACAAT TATGACCATC 
TCCTTGCTAG CACACCTTGC ATGTGAGAAG GGAATATGGG GTCCACATCT TATTGTAGTG 
CCAACTAGTG TTATGCTAAA TTGGGAGACT GAATTTCTCA AATGGTGTCC TGCGTTTAAA 
ATACTGACAT ATTTTGGAAG TGCAAAAGAG AGAAAACAGA AACGTCAAGG TTGGATGAAA 
CCAAATTACT TCCATGTATG CATCACTACA TACAGGCTTG TTATTCAGGA CTCCAAAGTG 
TTTAAGCGAA AAAAATGGAA GTATCTTATT CTTGATGAGG CTCATTTGAT AAAGAACTGG 
AAATCACAAA GATGGCAGAC TTTACTAAAT TTTAATTCGA AGCGACGCAT TCTTTTAACT 
GGAACTCCTC TACAAAATGA CCTCATGGAA CTTTGGTCTC TCATGCACTT TTTGATGCCT 
CATGTATTTC AGTCACATCA AGAGTTCAAG GACTGGTTCT GCAATCCAAT TTCAGGCATG 
GTAGAGGGCC AAGACAAGGT AAACAAAGAA GTGATAGATC GGTTGCACAA CGTCCTTCGT 
CCGTTTATAC TTCGTCGATT GAAACGGGAT GTGGAGAAAC AGTTGCCACA GAAGCATGAG 
CATGTCATAT ATTGCCGACT TTCCAGAAGG CAAAGGAACT TGTATGAAGA TTTCATTGCA 
AGCTCAGAGA CACAGGCAAC ATTGGCAAGT GGAAATTATT TTGGCATGAT TAGCATCATT 
ATGCAGCTTA GAAAGGTTTG TAACCATCCA GATCTTTTTG AAGGCCGTCC AATTATCAGC 
TCTTTTGACA TGGCAGGGAT CAACATGCAA ATCAGCTCTT CTGTTGGCAT GGTCCTTGAT 
AAAGGCCCAT TTTCTCAGGT TGACCTGTCA GATATGAATT TTGTATTTAC TCAAAATGAA 
TATAATATGA CTTCTTGGGA GGAAGATGAG GTTGCTGCCA TTTTTTCTCC AAGCATAACC 
CTCAGGGGTT CTGGCATTTC TCGCTCTACT AATGATGGTC AGAGAAGCAA TGGGACAAAT 
ATTTTTGAAG AGATTCACAA TTCTTTGTGG GAGGAGAGAA TAAAAGAAGC TAATGAAAGA 
GCAGCTTCCA TTGCATGGTG GAATAGGGTA AGATGCCAGA AGAGGCCTGT TTATGGCACA 
AATATTCGGG AGGTTTTGAC CATAAAGCAT CCTGTGTCTG ATATTCTCGA GAAGAAGAAG 
AACCCTTTAT GCCACATGGA ATTTTCATCA AGCCTAGCAA ATCTTGTTCT ATCATCGGTG 
GATCGCTTCA AGACAATGCT TGATTTTATT GAGTCATTTA CTTTTGCAAT TCCTGCTGCA 
CGAGCGCCTG CACCATTATT CTGGTGCAAC AAAGGGAAAT CTCCTGTTCT TATTGAACCA 
GCATACAGAG AGAAATGCAT GAATGAGTTT TCTCCTGTTT TCTCTCCTAT AAGGCCTGCG 
ATTGTTCGCC GTCAAGTGTA CTTTCCTGAC AGGCGTTTGA TCCAGTTTGA CTGTGGAAAG 
TTGCAGGAAC TTGCTATTTT GTTGAGGCGT TTGAAGTCAG AAGGACACAG GGCCTTGATA 
TTTACTCAGA TGACTAAGAT GCTTGATATA CTGGAGGAGT TCATTAATCT ATATGGGTAT 
ACGTATTTGC GGTTAGATGG ATCAACCCAG CCAGAAGAGA GGCAGACACT GATGCAGAGG 
TTTAATACTA ACCCAAAGTT TTTTCTTTTT ATCTTATCCA CTCGTAGTGG TGGTGTGGGA 
ATCAACCTAG TAGGTGCAGA CACTGTCATT TTCTATGATA GTGACTGGAA TCCTGCAATG 
GACCAACAGG CCCAAGACAG ATGCCACAGG ATAGGTCAAA CTCGTGAAGT TCACATCTAT 
AGACTGATCA GTGAGAGCAC TATAGAGGAG AATATACTCA AGAAAGCCAA TCAGAAGCGA 
GCTCTTGATG ATCTAGTGAT ACAACGCGGT AGCTACAATA CAGAGTTCTT CAAGAAGCTT 
GATCCTATGG AATTCTTTTC TGGGCACTCA TCACTTCATG CAGAGAATCA GCAGAAGGAT 
TGCTCTCTGT CTGCTGGACC CTCAAACGGA ACTAATCTGG CGCTGTCAAA TGTTGATGTT 
GAAGCAGCTA TTAGACAGGC AGAAGATGAA GCTGACTATA TGGCTCTCAA GAGGCTGGAG 
CAGGAAGAGG CCGTGGACAA TCAAGAGTTC AGCGAGGAGG CTGCTGGAAG ACTTGAGGAG 
GATGATCTTG TAAACGAGGA TGACACTAAA CCTGATGAAC ACACCAATGA AGAGCATAAA 
TATCAGTGTT CTGATTTGGT CAAGGACAAG CATGTTGCTT TATCTATAAA TCAATTAGAT 
GAAGAAAAGG CTATAACTTT GGCTGGAGGA GATGGAGATA TTGACATGCT TGCTGATGTT 
AAGCAGATGG CTGCTGCAGC TGCTGCAGCA GGTCAAGCAA GTTCGTCTTT TGAAAATCAG 
CTCCGGCCAA TTGATAGATA TGCCATGCGG TTTTTGGAAC TCTGGGATCC AATAATTGAT 
AAAGCTGCTA TAAACTATCA GGTCAATGTT GAAGAGGAAG AATGGGAACT TGAACGCATT 
GAAAAACTCA AAGAAGATTT AGAAGCAGAA ATTGATGAAG ACCAGGAACC ACTTTCTTAT 
GAGTCATGGG ATGTTGATTT TGCTACTACA GCATATCGGC AACATGTTGA GGCTCTAGCT 
CAAAAACAGT TGTTTGAGGA ACAAGAAAGA CAAGCCCGTG AAGCAGCAAA AGAGTTGGAG 
GAAAAGAATG ATAATAGCAC TCAACGAAAA AAGTCAAAAA AGAACAAGAA GAAGGCAGCG 
AAGTTCAAAT CTTTGAAGAA AGGACGTTTA TCATCTGAGT CAGAGGTTAT GGTTGAGGAA 
ACATCAGTAG ATACTATGAG CATTGATGAC AATGCACCCT CACCAGAGCT AATGAGCGAT 
GAATCAGCAC ACCATCATTC TAACAAGCAT AAGAGGATAA TGTCAACCAA TGAGGAAGAG 
AACAGCAATA GCAGAAGCCT GAAGAAGCTC AAGAAAGCCC CTAAATCGAG TTTCAGTTCC 
GAGGCCTTAT CACCTAAGCA TTTTCTTGAA GGCAAGCAGC TTAAGTTGAA AGATGAACTT 
AATGATTCTG ATCCCAAATC GGGGGCAAGA ATTAAGAGTG ATGGCCGAAT TTCTGTCCCA 
TGCATGCCAG TAAAGCGTGT CATGGTAATC AAGCCTGAGA GATTGAAGAA GAAGAGCCTA 
TGGTCTCGCG ATTGTGCTTC AGACTCGTGG ACAACTGAGG AAGATGCTGT TCTTTGTGCA 
ACTGTAAATG AGTACGGCCC TCTTTGGGAA CTGGCTAGTG ATTCTCTTCA TTCTGTACCA 
GGTGGTGCAT TTTATAGAGG AAGATACCGT CACCCTGTGC ATTGCTGTGA GAGATTCCGG 
GAATTAGTCT GCAAGCACAT CTTATCAGCA ACAGACAATT CAAACAGTGA GAAGGTTCCT 
TCTGGGACTG GGAAGGCTAT CTTGAAAGTA TCTGAGGATC AAACTCAGAT GTTGCTCAAT 
GTAATCAGCG AGCTTCCTAA CAATGAATTG CTTCTCCAGA AACATTTCAT GGCTGTTCTT 
TCATCTGTTT GGAGATCGAA GTCTGCCTGT GAGTCCCACT GTGTTATGAG TTACTCTAAC 
ACACTCCAGA AACCTGGCAG ACTCAGCGAG AACTGGTCCA TGACAAACTT CAGACCAAAC 
TTTAATCTAG TAAGGACAGC CCTCGCAGAT GCTCAGGTTC AATGTCCAAG AATGGTAGTT 
CCAACAAGCA ATCATGAGTC CCGCCGTAAC TTTTTGGAAT TGGAATTGGA TTTCTTGACA 
GATCGAGATG ATTATGAGGC GGACTTTCCA TCTGTTGTTA ACGTGTCCAT ACTAGAACCG 
GAACCATTAA AACATGCTGT GGAGCCAGTG GAGCACTCAC TACTGTCCAC ACTTTCTTAT 
AGACATGCTG AGAACAGATT CAGGATGGTA TCAGAAACTT GTTTTGAAGG GGAAGGTTCT 
CACTGGGCCT CATCTGCTTT CCACACATAC GATGCCGGTC GGCATAAATC TGGTCCTAAG 
TCCATAGGAA AGCACAAAGC ATCATCAGAA TCTGGAAGGC CTCCCAAATC CAAAATTCAA 
AGGACCACTG AACCACAGGA AGTGCCGGTT ACAAATAATT TTCATAGGAT TCCGGGGCAA 
CTTCTGCATA ACTCAGCAGA GTTCCACATT ACCCAGTCCC TATCTGACCT TGGCATCAGT 
GATTCTGAAT TCACCTACTT TGATAATCTT CCTCAAGAGG CTGAAACAGA GTTTGTTCCG 
TACCAGTATG ACTCTGATGT CCTTTCTGGC ATTGAGGAGT TGGATCCTCT TACAGATTTC 
ACAGACATCG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4330371 ( XM_015770118.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015625604.1 Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X1, mRNA.

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CloneID	OOa28159
Clone ID Related Accession (Same CDS sequence)	XM_015770118.2 , XM_015770119.1 , XM_015770118.1
Accession Version	XM_015770118.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379175.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCAA AAGGTCCTCG ATCAAAGTTA GATCATGAGA CAAGAGCCAG ACGCCAGAAG 
GCTCTTGAAG CCCCAAGGGG GGAGCCTCGA AGGACGAAAA CTCACTGGGA CCATCTTCTT 
GGAGAAATGG CTTGGCTGGC AAAGGAGTTT GATGCTGAGA GAAAATGGAA GCTGTCCATG 
GCTAAAAGGA TTGCTCAAAG AGCTAATAAG GGTGTAGTTG ACCAAGCAAC AAAGGACGAG 
AGAAAACAGA AGGAGGAAGA AGTTCGCCTG AGAAAAGTTG CCCTAAATAT TTCTAAGGAC 
GTGAAGAAGT TCTGGACCAA AATAGAGAAG CTGGTTTTTT ATAAGAACCA ACTGGAGCTT 
GAGGAAAGGA AAAAAAAGGC CCTAGACAAG CAACTTGATT TCCTCTTAGG ACAGACTGAA 
AGATATTCAA CAATGCTGGC AGAAAATCTT GTTGATGTGC GCCTTCAAAA TCAAGAAAAT 
GACTCTTTAC AGACTAATCA AAGATCCCAG CAGGAACTAG CACAAGAGAA CATAAATGCA 
TCAAGTCCGA CTGATGTTGA CAATGTGGAA ATCGATGATG ATTACAACAG TTCCTTGGGT 
GAAGAGCCAA AAGATGATGA ACACACAATC GATGAGGATG AAGCTCAAAT TACGGAGGCT 
GAGCGCAACG AGGAATTGGC TGCTTTGCAG GCAGAAGCTG ATCTACCACT GGATGATATT 
CTCAAGTTGT ACACTAAAAA TAAAGTTAGC AGGGAAAGCA GTCCAGATGG CAGAGACGTT 
TTTAGTGACT CAGATTCAAA GGATTTGATT AAAGATCCTT TGAACCAGGC TAATGGATGC 
AACGATGAAT CTGATCATAC TTCAAGTGAT GAAGGCATTT CTTCTGAGGA AGCAGATGAT 
TACCAGTCTT ACTCTGAGTT TGTGAAAAAG AACACTGTGA AATGCAATGG CAATATCTCA 
TCTGTTGATG CAAAGGACGA CGAAGATTAT GTTGCTAATG ATGAAGGAAA GGATGATGAA 
GCTACTTTGT CTGAAGAGGA AGAGTTGGCG AAGAAAGAAG ACTGTGATCC TCTAGATGAG 
GTTAAGTTAC TGCAGAAAGA GAGTGAGATC CCACTTGAAG AACTTCTTGC AAGATACCAA 
AAGGATGGCT ATGCAGATGA TGACACAACA GAATTGGAAA ATTCACCTGC ACTATCAGTT 
GAGGATGTCA ATGCCAACAT GCCTGTGGAT GATGAGTCTG CAGACACCGT GGAGGTAAAC 
AGGGATCTTT CTGCAGATAC CATGAAACTG ACCAGAGATC AGTCTGCAGA GACCGTAAAA 
GTGAATAATG ACCAGTCTGC AGAGATTGTG GAGGGCAACA ATGACACATT TGAAGATCAT 
GAATCTGCAG GCATGCTGGG ACCTGAGCAT GTCAGTGGTA GTGTTCTACA ACTAGAAACA 
TCAGAGCCTA TTGTACAGGA AAATACTGCT AAAGAGGGTG ATGTAACTGA TACTAAAGCG 
ATGGCCAATG GAGATAATAG TGATGTGATT GCTGATGCTG CAGCTGCTGC AAGATCAGCA 
CAACCGACTG GTAACACGTT CTCAACAACA AATGTGCGGA CAAAATTCCC TTTCCTTCTT 
AAGCATTCTC TACGAGAATA TCAGCACATT GGACTGGACT GGCTTGTTGC TATGTATGAA 
AAGAGGCTGA ATGGAATTCT AGCAGATGAA ATGGGTTTGG GGAAGACAAT TATGACCATC 
TCCTTGCTAG CACACCTTGC ATGTGAGAAG GGAATATGGG GTCCACATCT TATTGTAGTG 
CCAACTAGTG TTATGCTAAA TTGGGAGACT GAATTTCTCA AATGGTGTCC TGCGTTTAAA 
ATACTGACAT ATTTTGGAAG TGCAAAAGAG AGAAAACAGA AACGTCAAGG TTGGATGAAA 
CCAAATTACT TCCATGTATG CATCACTACA TACAGGCTTG TTATTCAGGA CTCCAAAGTG 
TTTAAGCGAA AAAAATGGAA GTATCTTATT CTTGATGAGG CTCATTTGAT AAAGAACTGG 
AAATCACAAA GATGGCAGAC TTTACTAAAT TTTAATTCGA AGCGACGCAT TCTTTTAACT 
GGAACTCCTC TACAAAATGA CCTCATGGAA CTTTGGTCTC TCATGCACTT TTTGATGCCT 
CATGTATTTC AGTCACATCA AGAGTTCAAG GACTGGTTCT GCAATCCAAT TTCAGGCATG 
GTAGAGGGCC AAGACAAGGT AAACAAAGAA GTGATAGATC GGTTGCACAA CGTCCTTCGT 
CCGTTTATAC TTCGTCGATT GAAACGGGAT GTGGAGAAAC AGTTGCCACA GAAGCATGAG 
CATGTCATAT ATTGCCGACT TTCCAGAAGG CAAAGGAACT TGTATGAAGA TTTCATTGCA 
AGCTCAGAGA CACAGGCAAC ATTGGCAAGT GGAAATTATT TTGGCATGAT TAGCATCATT 
ATGCAGCTTA GAAAGGTTTG TAACCATCCA GATCTTTTTG AAGGCCGTCC AATTATCAGC 
TCTTTTGACA TGGCAGGGAT CAACATGCAA ATCAGCTCTT CTGTTGGCAT GGTCCTTGAT 
AAAGGCCCAT TTTCTCAGGT TGACCTGTCA GATATGAATT TTGTATTTAC TCAAAATGAA 
TATAATATGA CTTCTTGGGA GGAAGATGAG GTTGCTGCCA TTTTTTCTCC AAGCATAACC 
CTCAGGGGTT CTGGCATTTC TCGCTCTACT AATGATGGTC AGAGAAGCAA TGGGACAAAT 
ATTTTTGAAG AGATTCACAA TTCTTTGTGG GAGGAGAGAA TAAAAGAAGC TAATGAAAGA 
GCAGCTTCCA TTGCATGGTG GAATAGGGTA AGATGCCAGA AGAGGCCTGT TTATGGCACA 
AATATTCGGG AGGTTTTGAC CATAAAGCAT CCTGTGTCTG ATATTCTCGA GAAGAAGAAG 
AACCCTTTAT GCCACATGGA ATTTTCATCA AGCCTAGCAA ATCTTGTTCT ATCATCGGTG 
GATCGCTTCA AGACAATGCT TGATTTTATT GAGTCATTTA CTTTTGCAAT TCCTGCTGCA 
CGAGCGCCTG CACCATTATT CTGGTGCAAC AAAGGGAAAT CTCCTGTTCT TATTGAACCA 
GCATACAGAG AGAAATGCAT GAATGAGTTT TCTCCTGTTT TCTCTCCTAT AAGGCCTGCG 
ATTGTTCGCC GTCAAGTGTA CTTTCCTGAC AGGCGTTTGA TCCAGTTTGA CTGTGGAAAG 
TTGCAGGAAC TTGCTATTTT GTTGAGGCGT TTGAAGTCAG AAGGACACAG GGCCTTGATA 
TTTACTCAGA TGACTAAGAT GCTTGATATA CTGGAGGAGT TCATTAATCT ATATGGGTAT 
ACGTATTTGC GGTTAGATGG ATCAACCCAG CCAGAAGAGA GGCAGACACT GATGCAGAGG 
TTTAATACTA ACCCAAAGTT TTTTCTTTTT ATCTTATCCA CTCGTAGTGG TGGTGTGGGA 
ATCAACCTAG TAGGTGCAGA CACTGTCATT TTCTATGATA GTGACTGGAA TCCTGCAATG 
GACCAACAGG CCCAAGACAG ATGCCACAGG ATAGGTCAAA CTCGTGAAGT TCACATCTAT 
AGACTGATCA GTGAGAGCAC TATAGAGGAG AATATACTCA AGAAAGCCAA TCAGAAGCGA 
GCTCTTGATG ATCTAGTGAT ACAACGCGGT AGCTACAATA CAGAGTTCTT CAAGAAGCTT 
GATCCTATGG AATTCTTTTC TGGGCACTCA TCACTTCATG CAGAGAATCA GCAGAAGGAT 
TGCTCTCTGT CTGCTGGACC CTCAAACGGA ACTAATCTGG CGCTGTCAAA TGTTGATGTT 
GAAGCAGCTA TTAGACAGGC AGAAGATGAA GCTGACTATA TGGCTCTCAA GAGGCTGGAG 
CAGGAAGAGG CCGTGGACAA TCAAGAGTTC AGCGAGGAGG CTGCTGGAAG ACTTGAGGAG 
GATGATCTTG TAAACGAGGA TGACACTAAA CCTGATGAAC ACACCAATGA AGAGCATAAA 
TATCAGTGTT CTGATTTGGT CAAGGACAAG CATGTTGCTT TATCTATAAA TCAATTAGAT 
GAAGAAAAGG CTATAACTTT GGCTGGAGGA GATGGAGATA TTGACATGCT TGCTGATGTT 
AAGCAGATGG CTGCTGCAGC TGCTGCAGCA GGTCAAGCAA GTTCGTCTTT TGAAAATCAG 
CTCCGGCCAA TTGATAGATA TGCCATGCGG TTTTTGGAAC TCTGGGATCC AATAATTGAT 
AAAGCTGCTA TAAACTATCA GGTCAATGTT GAAGAGGAAG AATGGGAACT TGAACGCATT 
GAAAAACTCA AAGAAGATTT AGAAGCAGAA ATTGATGAAG ACCAGGAACC ACTTTCTTAT 
GAGTCATGGG ATGTTGATTT TGCTACTACA GCATATCGGC AACATGTTGA GGCTCTAGCT 
CAAAAACAGT TGTTTGAGGA ACAAGAAAGA CAAGCCCGTG AAGCAGCAAA AGAGTTGGAG 
GAAAAGAATG ATAATAGCAC TCAACGAAAA AAGTCAAAAA AGAACAAGAA GAAGGCAGCG 
AAGTTCAAAT CTTTGAAGAA AGGACGTTTA TCATCTGAGT CAGAGGTTAT GGTTGAGGAA 
ACATCAGTAG ATACTATGAG CATTGATGAC AATGCACCCT CACCAGAGCT AATGAGCGAT 
GAATCAGCAC ACCATCATTC TAACAAGCAT AAGAGGATAA TGTCAACCAA TGAGGAAGAG 
AACAGCAATA GCAGAAGCCT GAAGAAGCTC AAGAAAGCCC CTAAATCGAG TTTCAGTTCC 
GAGGCCTTAT CACCTAAGCA TTTTCTTGAA GGCAAGCAGC TTAAGTTGAA AGATGAACTT 
AATGATTCTG ATCCCAAATC GGGGGCAAGA ATTAAGAGTG ATGGCCGAAT TTCTGTCCCA 
TGCATGCCAG TAAAGCGTGT CATGGTAATC AAGCCTGAGA GATTGAAGAA GAAGAGCCTA 
TGGTCTCGCG ATTGTGCTTC AGACTCGTGG ACAACTGAGG AAGATGCTGT TCTTTGTGCA 
ACTGTAAATG AGTACGGCCC TCTTTGGGAA CTGGCTAGTG ATTCTCTTCA TTCTGTACCA 
GGTGGTGCAT TTTATAGAGG AAGATACCGT CACCCTGTGC ATTGCTGTGA GAGATTCCGG 
GAATTAGTCT GCAAGCACAT CTTATCAGCA ACAGACAATT CAAACAGTGA GAAGGTTCCT 
TCTGGGACTG GGAAGGCTAT CTTGAAAGTA TCTGAGGATC AAACTCAGAT GTTGCTCAAT 
GTAATCAGCG AGCTTCCTAA CAATGAATTG CTTCTCCAGA AACATTTCAT GGCTGTTCTT 
TCATCTGTTT GGAGATCGAA GTCTGCCTGT GAGTCCCACT GTGTTATGAG TTACTCTAAC 
ACACTCCAGA AACCTGGCAG ACTCAGCGAG AACTGGTCCA TGACAAACTT CAGACCAAAC 
TTTAATCTAG TAAGGACAGC CCTCGCAGAT GCTCAGGTTC AATGTCCAAG AATGGTAGTT 
CCAACAAGCA ATCATGAGTC CCGCCGTAAC TTTTTGGAAT TGGAATTGGA TTTCTTGACA 
GATCGAGATG ATTATGAGGC GGACTTTCCA TCTGTTGTTA ACGTGTCCAT ACTAGAACCG 
GAACCATTAA AACATGCTGT GGAGCCAGTG GAGCACTCAC TACTGTCCAC ACTTTCTTAT 
AGACATGCTG AGAACAGATT CAGGATGGTA TCAGAAACTT GTTTTGAAGG GGAAGGTTCT 
CACTGGGCCT CATCTGCTTT CCACACATAC GATGCCGGTC GGCATAAATC TGGTCCTAAG 
TCCATAGGAA AGCACAAAGC ATCATCAGAA TCTGGAAGGC CTCCCAAATC CAAAATTCAA 
AGGACCACTG AACCACAGGA AGTGCCGGTT ACAAATAATT TTCATAGGAT TCCGGGGCAA 
CTTCTGCATA ACTCAGCAGA GTTCCACATT ACCCAGTCCC TATCTGACCT TGGCATCAGT 
GATTCTGAAT TCACCTACTT TGATAATCTT CCTCAAGAGG CTGAAACAGA GTTTGTTCCG 
TACCAGTATG ACTCTGATGT CCTTTCTGGC ATTGAGGAGT TGGATCCTCT TACAGATTTC 
ACAGACATCG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015625604.1
CDS	416..6550
Translation	MASKGPRSKLDHETRARRQKALEAPRGEPRRTKTHWDHLLGEMAWLAKEFDAERKWKLSMAKRIAQRANKGVVDQATKDERKQKEEEVRLRKVALNISKDVKKFWTKIEKLVFYKNQLELEERKKKALDKQLDFLLGQTERYSTMLAENLVDVRLQNQENDSLQTNQRSQQELAQENINASSPTDVDNVEIDDDYNSSLGEEPKDDEHTIDEDEAQITEAERNEELAALQAEADLPLDDILKLYTKNKVSRESSPDGRDVFSDSDSKDLIKDPLNQANGCNDESDHTSSDEGISSEEADDYQSYSEFVKKNTVKCNGNISSVDAKDDEDYVANDEGKDDEATLSEEEELAKKEDCDPLDEVKLLQKESEIPLEELLARYQKDGYADDDTTELENSPALSVEDVNANMPVDDESADTVEVNRDLSADTMKLTRDQSAETVKVNNDQSAEIVEGNNDTFEDHESAGMLGPEHVSGSVLQLETSEPIVQENTAKEGDVTDTKAMANGDNSDVIADAAAAARSAQPTGNTFSTTNVRTKFPFLLKHSLREYQHIGLDWLVAMYEKRLNGILADEMGLGKTIMTISLLAHLACEKGIWGPHLIVVPTSVMLNWETEFLKWCPAFKILTYFGSAKERKQKRQGWMKPNYFHVCITTYRLVIQDSKVFKRKKWKYLILDEAHLIKNWKSQRWQTLLNFNSKRRILLTGTPLQNDLMELWSLMHFLMPHVFQSHQEFKDWFCNPISGMVEGQDKVNKEVIDRLHNVLRPFILRRLKRDVEKQLPQKHEHVIYCRLSRRQRNLYEDFIASSETQATLASGNYFGMISIIMQLRKVCNHPDLFEGRPIISSFDMAGINMQISSSVGMVLDKGPFSQVDLSDMNFVFTQNEYNMTSWEEDEVAAIFSPSITLRGSGISRSTNDGQRSNGTNIFEEIHNSLWEERIKEANERAASIAWWNRVRCQKRPVYGTNIREVLTIKHPVSDILEKKKNPLCHMEFSSSLANLVLSSVDRFKTMLDFIESFTFAIPAARAPAPLFWCNKGKSPVLIEPAYREKCMNEFSPVFSPIRPAIVRRQVYFPDRRLIQFDCGKLQELAILLRRLKSEGHRALIFTQMTKMLDILEEFINLYGYTYLRLDGSTQPEERQTLMQRFNTNPKFFLFILSTRSGGVGINLVGADTVIFYDSDWNPAMDQQAQDRCHRIGQTREVHIYRLISESTIEENILKKANQKRALDDLVIQRGSYNTEFFKKLDPMEFFSGHSSLHAENQQKDCSLSAGPSNGTNLALSNVDVEAAIRQAEDEADYMALKRLEQEEAVDNQEFSEEAAGRLEEDDLVNEDDTKPDEHTNEEHKYQCSDLVKDKHVALSINQLDEEKAITLAGGDGDIDMLADVKQMAAAAAAAGQASSSFENQLRPIDRYAMRFLELWDPIIDKAAINYQVNVEEEEWELERIEKLKEDLEAEIDEDQEPLSYESWDVDFATTAYRQHVEALAQKQLFEEQERQAREAAKELEEKNDNSTQRKKSKKNKKKAAKFKSLKKGRLSSESEVMVEETSVDTMSIDDNAPSPELMSDESAHHHSNKHKRIMSTNEEENSNSRSLKKLKKAPKSSFSSEALSPKHFLEGKQLKLKDELNDSDPKSGARIKSDGRISVPCMPVKRVMVIKPERLKKKSLWSRDCASDSWTTEEDAVLCATVNEYGPLWELASDSLHSVPGGAFYRGRYRHPVHCCERFRELVCKHILSATDNSNSEKVPSGTGKAILKVSEDQTQMLLNVISELPNNELLLQKHFMAVLSSVWRSKSACESHCVMSYSNTLQKPGRLSENWSMTNFRPNFNLVRTALADAQVQCPRMVVPTSNHESRRNFLELELDFLTDRDDYEADFPSVVNVSILEPEPLKHAVEPVEHSLLSTLSYRHAENRFRMVSETCFEGEGSHWASSAFHTYDAGRHKSGPKSIGKHKASSESGRPPKSKIQRTTEPQEVPVTNNFHRIPGQLLHNSAEFHITQSLSDLGISDSEFTYFDNLPQEAETEFVPYQYDSDVLSGIEELDPLTDFTDIG

Target ORF information:

RefSeq Version	XM_015770118.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015770118.1

ORF Insert Sequence:

ATGGCATCAA AAGGTCCTCG ATCAAAGTTA GATCATGAGA CAAGAGCCAG ACGCCAGAAG 
GCTCTTGAAG CCCCAAGGGG GGAGCCTCGA AGGACGAAAA CTCACTGGGA CCATCTTCTT 
GGAGAAATGG CTTGGCTGGC AAAGGAGTTT GATGCTGAGA GAAAATGGAA GCTGTCCATG 
GCTAAAAGGA TTGCTCAAAG AGCTAATAAG GGTGTAGTTG ACCAAGCAAC AAAGGACGAG 
AGAAAACAGA AGGAGGAAGA AGTTCGCCTG AGAAAAGTTG CCCTAAATAT TTCTAAGGAC 
GTGAAGAAGT TCTGGACCAA AATAGAGAAG CTGGTTTTTT ATAAGAACCA ACTGGAGCTT 
GAGGAAAGGA AAAAAAAGGC CCTAGACAAG CAACTTGATT TCCTCTTAGG ACAGACTGAA 
AGATATTCAA CAATGCTGGC AGAAAATCTT GTTGATGTGC GCCTTCAAAA TCAAGAAAAT 
GACTCTTTAC AGACTAATCA AAGATCCCAG CAGGAACTAG CACAAGAGAA CATAAATGCA 
TCAAGTCCGA CTGATGTTGA CAATGTGGAA ATCGATGATG ATTACAACAG TTCCTTGGGT 
GAAGAGCCAA AAGATGATGA ACACACAATC GATGAGGATG AAGCTCAAAT TACGGAGGCT 
GAGCGCAACG AGGAATTGGC TGCTTTGCAG GCAGAAGCTG ATCTACCACT GGATGATATT 
CTCAAGTTGT ACACTAAAAA TAAAGTTAGC AGGGAAAGCA GTCCAGATGG CAGAGACGTT 
TTTAGTGACT CAGATTCAAA GGATTTGATT AAAGATCCTT TGAACCAGGC TAATGGATGC 
AACGATGAAT CTGATCATAC TTCAAGTGAT GAAGGCATTT CTTCTGAGGA AGCAGATGAT 
TACCAGTCTT ACTCTGAGTT TGTGAAAAAG AACACTGTGA AATGCAATGG CAATATCTCA 
TCTGTTGATG CAAAGGACGA CGAAGATTAT GTTGCTAATG ATGAAGGAAA GGATGATGAA 
GCTACTTTGT CTGAAGAGGA AGAGTTGGCG AAGAAAGAAG ACTGTGATCC TCTAGATGAG 
GTTAAGTTAC TGCAGAAAGA GAGTGAGATC CCACTTGAAG AACTTCTTGC AAGATACCAA 
AAGGATGGCT ATGCAGATGA TGACACAACA GAATTGGAAA ATTCACCTGC ACTATCAGTT 
GAGGATGTCA ATGCCAACAT GCCTGTGGAT GATGAGTCTG CAGACACCGT GGAGGTAAAC 
AGGGATCTTT CTGCAGATAC CATGAAACTG ACCAGAGATC AGTCTGCAGA GACCGTAAAA 
GTGAATAATG ACCAGTCTGC AGAGATTGTG GAGGGCAACA ATGACACATT TGAAGATCAT 
GAATCTGCAG GCATGCTGGG ACCTGAGCAT GTCAGTGGTA GTGTTCTACA ACTAGAAACA 
TCAGAGCCTA TTGTACAGGA AAATACTGCT AAAGAGGGTG ATGTAACTGA TACTAAAGCG 
ATGGCCAATG GAGATAATAG TGATGTGATT GCTGATGCTG CAGCTGCTGC AAGATCAGCA 
CAACCGACTG GTAACACGTT CTCAACAACA AATGTGCGGA CAAAATTCCC TTTCCTTCTT 
AAGCATTCTC TACGAGAATA TCAGCACATT GGACTGGACT GGCTTGTTGC TATGTATGAA 
AAGAGGCTGA ATGGAATTCT AGCAGATGAA ATGGGTTTGG GGAAGACAAT TATGACCATC 
TCCTTGCTAG CACACCTTGC ATGTGAGAAG GGAATATGGG GTCCACATCT TATTGTAGTG 
CCAACTAGTG TTATGCTAAA TTGGGAGACT GAATTTCTCA AATGGTGTCC TGCGTTTAAA 
ATACTGACAT ATTTTGGAAG TGCAAAAGAG AGAAAACAGA AACGTCAAGG TTGGATGAAA 
CCAAATTACT TCCATGTATG CATCACTACA TACAGGCTTG TTATTCAGGA CTCCAAAGTG 
TTTAAGCGAA AAAAATGGAA GTATCTTATT CTTGATGAGG CTCATTTGAT AAAGAACTGG 
AAATCACAAA GATGGCAGAC TTTACTAAAT TTTAATTCGA AGCGACGCAT TCTTTTAACT 
GGAACTCCTC TACAAAATGA CCTCATGGAA CTTTGGTCTC TCATGCACTT TTTGATGCCT 
CATGTATTTC AGTCACATCA AGAGTTCAAG GACTGGTTCT GCAATCCAAT TTCAGGCATG 
GTAGAGGGCC AAGACAAGGT AAACAAAGAA GTGATAGATC GGTTGCACAA CGTCCTTCGT 
CCGTTTATAC TTCGTCGATT GAAACGGGAT GTGGAGAAAC AGTTGCCACA GAAGCATGAG 
CATGTCATAT ATTGCCGACT TTCCAGAAGG CAAAGGAACT TGTATGAAGA TTTCATTGCA 
AGCTCAGAGA CACAGGCAAC ATTGGCAAGT GGAAATTATT TTGGCATGAT TAGCATCATT 
ATGCAGCTTA GAAAGGTTTG TAACCATCCA GATCTTTTTG AAGGCCGTCC AATTATCAGC 
TCTTTTGACA TGGCAGGGAT CAACATGCAA ATCAGCTCTT CTGTTGGCAT GGTCCTTGAT 
AAAGGCCCAT TTTCTCAGGT TGACCTGTCA GATATGAATT TTGTATTTAC TCAAAATGAA 
TATAATATGA CTTCTTGGGA GGAAGATGAG GTTGCTGCCA TTTTTTCTCC AAGCATAACC 
CTCAGGGGTT CTGGCATTTC TCGCTCTACT AATGATGGTC AGAGAAGCAA TGGGACAAAT 
ATTTTTGAAG AGATTCACAA TTCTTTGTGG GAGGAGAGAA TAAAAGAAGC TAATGAAAGA 
GCAGCTTCCA TTGCATGGTG GAATAGGGTA AGATGCCAGA AGAGGCCTGT TTATGGCACA 
AATATTCGGG AGGTTTTGAC CATAAAGCAT CCTGTGTCTG ATATTCTCGA GAAGAAGAAG 
AACCCTTTAT GCCACATGGA ATTTTCATCA AGCCTAGCAA ATCTTGTTCT ATCATCGGTG 
GATCGCTTCA AGACAATGCT TGATTTTATT GAGTCATTTA CTTTTGCAAT TCCTGCTGCA 
CGAGCGCCTG CACCATTATT CTGGTGCAAC AAAGGGAAAT CTCCTGTTCT TATTGAACCA 
GCATACAGAG AGAAATGCAT GAATGAGTTT TCTCCTGTTT TCTCTCCTAT AAGGCCTGCG 
ATTGTTCGCC GTCAAGTGTA CTTTCCTGAC AGGCGTTTGA TCCAGTTTGA CTGTGGAAAG 
TTGCAGGAAC TTGCTATTTT GTTGAGGCGT TTGAAGTCAG AAGGACACAG GGCCTTGATA 
TTTACTCAGA TGACTAAGAT GCTTGATATA CTGGAGGAGT TCATTAATCT ATATGGGTAT 
ACGTATTTGC GGTTAGATGG ATCAACCCAG CCAGAAGAGA GGCAGACACT GATGCAGAGG 
TTTAATACTA ACCCAAAGTT TTTTCTTTTT ATCTTATCCA CTCGTAGTGG TGGTGTGGGA 
ATCAACCTAG TAGGTGCAGA CACTGTCATT TTCTATGATA GTGACTGGAA TCCTGCAATG 
GACCAACAGG CCCAAGACAG ATGCCACAGG ATAGGTCAAA CTCGTGAAGT TCACATCTAT 
AGACTGATCA GTGAGAGCAC TATAGAGGAG AATATACTCA AGAAAGCCAA TCAGAAGCGA 
GCTCTTGATG ATCTAGTGAT ACAACGCGGT AGCTACAATA CAGAGTTCTT CAAGAAGCTT 
GATCCTATGG AATTCTTTTC TGGGCACTCA TCACTTCATG CAGAGAATCA GCAGAAGGAT 
TGCTCTCTGT CTGCTGGACC CTCAAACGGA ACTAATCTGG CGCTGTCAAA TGTTGATGTT 
GAAGCAGCTA TTAGACAGGC AGAAGATGAA GCTGACTATA TGGCTCTCAA GAGGCTGGAG 
CAGGAAGAGG CCGTGGACAA TCAAGAGTTC AGCGAGGAGG CTGCTGGAAG ACTTGAGGAG 
GATGATCTTG TAAACGAGGA TGACACTAAA CCTGATGAAC ACACCAATGA AGAGCATAAA 
TATCAGTGTT CTGATTTGGT CAAGGACAAG CATGTTGCTT TATCTATAAA TCAATTAGAT 
GAAGAAAAGG CTATAACTTT GGCTGGAGGA GATGGAGATA TTGACATGCT TGCTGATGTT 
AAGCAGATGG CTGCTGCAGC TGCTGCAGCA GGTCAAGCAA GTTCGTCTTT TGAAAATCAG 
CTCCGGCCAA TTGATAGATA TGCCATGCGG TTTTTGGAAC TCTGGGATCC AATAATTGAT 
AAAGCTGCTA TAAACTATCA GGTCAATGTT GAAGAGGAAG AATGGGAACT TGAACGCATT 
GAAAAACTCA AAGAAGATTT AGAAGCAGAA ATTGATGAAG ACCAGGAACC ACTTTCTTAT 
GAGTCATGGG ATGTTGATTT TGCTACTACA GCATATCGGC AACATGTTGA GGCTCTAGCT 
CAAAAACAGT TGTTTGAGGA ACAAGAAAGA CAAGCCCGTG AAGCAGCAAA AGAGTTGGAG 
GAAAAGAATG ATAATAGCAC TCAACGAAAA AAGTCAAAAA AGAACAAGAA GAAGGCAGCG 
AAGTTCAAAT CTTTGAAGAA AGGACGTTTA TCATCTGAGT CAGAGGTTAT GGTTGAGGAA 
ACATCAGTAG ATACTATGAG CATTGATGAC AATGCACCCT CACCAGAGCT AATGAGCGAT 
GAATCAGCAC ACCATCATTC TAACAAGCAT AAGAGGATAA TGTCAACCAA TGAGGAAGAG 
AACAGCAATA GCAGAAGCCT GAAGAAGCTC AAGAAAGCCC CTAAATCGAG TTTCAGTTCC 
GAGGCCTTAT CACCTAAGCA TTTTCTTGAA GGCAAGCAGC TTAAGTTGAA AGATGAACTT 
AATGATTCTG ATCCCAAATC GGGGGCAAGA ATTAAGAGTG ATGGCCGAAT TTCTGTCCCA 
TGCATGCCAG TAAAGCGTGT CATGGTAATC AAGCCTGAGA GATTGAAGAA GAAGAGCCTA 
TGGTCTCGCG ATTGTGCTTC AGACTCGTGG ACAACTGAGG AAGATGCTGT TCTTTGTGCA 
ACTGTAAATG AGTACGGCCC TCTTTGGGAA CTGGCTAGTG ATTCTCTTCA TTCTGTACCA 
GGTGGTGCAT TTTATAGAGG AAGATACCGT CACCCTGTGC ATTGCTGTGA GAGATTCCGG 
GAATTAGTCT GCAAGCACAT CTTATCAGCA ACAGACAATT CAAACAGTGA GAAGGTTCCT 
TCTGGGACTG GGAAGGCTAT CTTGAAAGTA TCTGAGGATC AAACTCAGAT GTTGCTCAAT 
GTAATCAGCG AGCTTCCTAA CAATGAATTG CTTCTCCAGA AACATTTCAT GGCTGTTCTT 
TCATCTGTTT GGAGATCGAA GTCTGCCTGT GAGTCCCACT GTGTTATGAG TTACTCTAAC 
ACACTCCAGA AACCTGGCAG ACTCAGCGAG AACTGGTCCA TGACAAACTT CAGACCAAAC 
TTTAATCTAG TAAGGACAGC CCTCGCAGAT GCTCAGGTTC AATGTCCAAG AATGGTAGTT 
CCAACAAGCA ATCATGAGTC CCGCCGTAAC TTTTTGGAAT TGGAATTGGA TTTCTTGACA 
GATCGAGATG ATTATGAGGC GGACTTTCCA TCTGTTGTTA ACGTGTCCAT ACTAGAACCG 
GAACCATTAA AACATGCTGT GGAGCCAGTG GAGCACTCAC TACTGTCCAC ACTTTCTTAT 
AGACATGCTG AGAACAGATT CAGGATGGTA TCAGAAACTT GTTTTGAAGG GGAAGGTTCT 
CACTGGGCCT CATCTGCTTT CCACACATAC GATGCCGGTC GGCATAAATC TGGTCCTAAG 
TCCATAGGAA AGCACAAAGC ATCATCAGAA TCTGGAAGGC CTCCCAAATC CAAAATTCAA 
AGGACCACTG AACCACAGGA AGTGCCGGTT ACAAATAATT TTCATAGGAT TCCGGGGCAA 
CTTCTGCATA ACTCAGCAGA GTTCCACATT ACCCAGTCCC TATCTGACCT TGGCATCAGT 
GATTCTGAAT TCACCTACTT TGATAATCTT CCTCAAGAGG CTGAAACAGA GTTTGTTCCG 
TACCAGTATG ACTCTGATGT CCTTTCTGGC ATTGAGGAGT TGGATCCTCT TACAGATTTC 
ACAGACATCG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4330371 ( XM_026022820.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_025878605.1 Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X1, mRNA.

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CloneID	OOa45722
Clone ID Related Accession (Same CDS sequence)	XM_026022820.1 , XM_026022821.1
Accession Version	XM_026022820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029257.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCAA AAGGTCCTCG ATCAAAGTTA GATCATGAGA CAAGAGCCAG ACGCCAGAAG 
GCTCTTGAAG CCCCAAGGGG GGAGCCTCGA AGGACGAAAA CTCACTGGGA CCATCTTCTT 
GGAGAAATGG CTTGGCTGGC AAAGGAGTTT GATGCTGAGA GAAAATGGAA GCTGTCCATG 
GCTAAAAGGA TTGCTCAAAG AGCTAATAAG GGTGTAGTTG ACCAAGCAAC AAAGGACGAG 
AGAAAACAGA AGGAGGAAGA AGTTCGCCTG AGAAAAGTTG CCCTAAATAT TTCTAAGGAC 
GTGAAGAAGT TCTGGACCAA AATAGAGAAG CTGGTTTTTT ATAAGAACCA ACTGGAGCTT 
GAGGAAAGGA AAAAAAAGGC CCTAGACAAG CAACTTGATT TCCTCTTAGG ACAGACTGAA 
AGATATTCAA CAATGCTGGC AGAAAATCTT GTTGATGTGC GCCTTCAAAA TCAAGAAAAT 
GACTCTTTAC AGACTAATCA AAGATCCCAG CAGGAACTAG CACAAGAGAA CATAAATGCA 
TCAAGTCCGA CTGATGTTGA CAATGTGGAA ATCGATGATG ATTACAACAG TTCCTTGGGT 
GAAGAGCCAA AAGATGATGA ACACACAATC GATGAGGATG AAGCTCAAAT TACGGAGGCT 
GAGCGCAACG AGGAATTGGC TGCTTTGCAG GCAGAAGCTG ATCTACCACT GGATGATATT 
CTCAAGTTGT ACACTAAAAA TAAAGTTCAA GCTTTCTCAG TTAGCAGGGA AAGCAGTCCA 
GATGGCAGAG ACGTTTTTAG TGACTCAGAT TCAAAGGATT TGATTAAAGA TCCTTTGAAC 
CAGGCTAATG GATGCAACGA TGAATCTGAT CATACTTCAA GTGATGAAGG CATTTCTTCT 
GAGGAAGCAG ATGATTACCA GTCTTACTCT GAGTTTGTGA AAAAGAACAC TGTGAAATGC 
AATGGCAATA TCTCATCTGT TGATGCAAAG GACGACGAAG ATTATGTTGC TAATGATGAA 
GGAAAGGATG ATGAAGCTAC TTTGTCTGAA GAGGAAGAGT TGGCGAAGAA AGAAGACTGT 
GATCCTCTAG ATGAGGTTAA GTTACTGCAG AAAGAGAGTG AGATCCCACT TGAAGAACTT 
CTTGCAAGAT ACCAAAAGGA TGGCTATGCA GATGATGACA CAACAGAATT GGAAAATTCA 
CCTGCACTAT CAGTTGAGGA TGTCAATGCC AACATGCCTG TGGATGATGA GTCTGCAGAC 
ACCGTGGAGG TAAACAGGGA TCTTTCTGCA GATACCATGA AACTGACCAG AGATCAGTCT 
GCAGAGACCG TAAAAGTGAA TAATGACCAG TCTGCAGAGA TTGTGGAGGG CAACAATGAC 
ACATTTGAAG ATCATGAATC TGCAGGCATG CTGGGACCTG AGCATGTCAG TGGTAGTGTT 
CTACAACTAG AAACATCAGA GCCTATTGTA CAGGAAAATA CTGCTAAAGA GGGTGATGTA 
ACTGATACTA AAGCGATGGC CAATGGAGAT AATAGTGATG TGATTGCTGA TGCTGCAGCT 
GCTGCAAGAT CAGCACAACC GACTGGTAAC ACGTTCTCAA CAACAAATGT GCGGACAAAA 
TTCCCTTTCC TTCTTAAGCA TTCTCTACGA GAATATCAGC ACATTGGACT GGACTGGCTT 
GTTGCTATGT ATGAAAAGAG GCTGAATGGA ATTCTAGCAG ATGAAATGGG TTTGGGGAAG 
ACAATTATGA CCATCTCCTT GCTAGCACAC CTTGCATGTG AGAAGGGAAT ATGGGGTCCA 
CATCTTATTG TAGTGCCAAC TAGTGTTATG CTAAATTGGG AGACTGAATT TCTCAAATGG 
TGTCCTGCGT TTAAAATACT GACATATTTT GGAAGTGCAA AAGAGAGAAA ACAGAAACGT 
CAAGGTTGGA TGAAACCAAA TTACTTCCAT GTATGCATCA CTACATACAG GCTTGTTATT 
CAGGACTCCA AAGTGTTTAA GCGAAAAAAA TGGAAGTATC TTATTCTTGA TGAGGCTCAT 
TTGATAAAGA ACTGGAAATC ACAAAGATGG CAGACTTTAC TAAATTTTAA TTCGAAGCGA 
CGCATTCTTT TAACTGGAAC TCCTCTACAA AATGACCTCA TGGAACTTTG GTCTCTCATG 
CACTTTTTGA TGCCTCATGT ATTTCAGTCA CATCAAGAGT TCAAGGACTG GTTCTGCAAT 
CCAATTTCAG GCATGGTAGA GGGCCAAGAC AAGGTAAACA AAGAAGTGAT AGATCGGTTG 
CACAACGTCC TTCGTCCGTT TATACTTCGT CGATTGAAAC GGGATGTGGA GAAACAGTTG 
CCACAGAAGC ATGAGCATGT CATATATTGC CGACTTTCCA GAAGGCAAAG GAACTTGTAT 
GAAGATTTCA TTGCAAGCTC AGAGACACAG GCAACATTGG CAAGTGGAAA TTATTTTGGC 
ATGATTAGCA TCATTATGCA GCTTAGAAAG GTTTGTAACC ATCCAGATCT TTTTGAAGGC 
CGTCCAATTA TCAGCTCTTT TGACATGGCA GGGATCAACA TGCAAATCAG CTCTTCTGTT 
GGCATGGTCC TTGATAAAGG CCCATTTTCT CAGGTTGACC TGTCAGATAT GAATTTTGTA 
TTTACTCAAA ATGAATATAA TATGACTTCT TGGGAGGAAG ATGAGGTTGC TGCCATTTTT 
TCTCCAAGCA TAACCCTCAG GGGTTCTGGC ATTTCTCGCT CTACTAATGA TGGTCAGAGA 
AGCAATGGGA CAAATATTTT TGAAGAGATT CACAATTCTT TGTGGGAGGA GAGAATAAAA 
GAAGCTAATG AAAGAGCAGC TTCCATTGCA TGGTGGAATA GGGTAAGATG CCAGAAGAGG 
CCTGTTTATG GCACAAATAT TCGGGAGGTT TTGACCATAA AGCATCCTGT GTCTGATATT 
CTCGAGAAGA AGAAGAACCC TTTATGCCAC ATGGAATTTT CATCAAGCCT AGCAAATCTT 
GTTCTATCAT CGGTGGATCG CTTCAAGACA ATGCTTGATT TTATTGAGTC ATTTACTTTT 
GCAATTCCTG CTGCACGAGC GCCTGCACCA TTATTCTGGT GCAACAAAGG GAAATCTCCT 
GTTCTTATTG AACCAGCATA CAGAGAGAAA TGCATGAATG AGTTTTCTCC TGTTTTCTCT 
CCTATAAGGC CTGCGATTGT TCGCCGTCAA GTGTACTTTC CTGACAGGCG TTTGATCCAG 
TTTGACTGTG GAAAGTTGCA GGAACTTGCT ATTTTGTTGA GGCGTTTGAA GTCAGAAGGA 
CACAGGGCCT TGATATTTAC TCAGATGACT AAGATGCTTG ATATACTGGA GGAGTTCATT 
AATCTATATG GGTATACGTA TTTGCGGTTA GATGGATCAA CCCAGCCAGA AGAGAGGCAG 
ACACTGATGC AGAGGTTTAA TACTAACCCA AAGTTTTTTC TTTTTATCTT ATCCACTCGT 
AGTGGTGGTG TGGGAATCAA CCTAGTAGGT GCAGACACTG TCATTTTCTA TGATAGTGAC 
TGGAATCCTG CAATGGACCA ACAGGCCCAA GACAGATGCC ACAGGATAGG TCAAACTCGT 
GAAGTTCACA TCTATAGACT GATCAGTGAG AGCACTATAG AGGAGAATAT ACTCAAGAAA 
GCCAATCAGA AGCGAGCTCT TGATGATCTA GTGATACAAC GCGGTAGCTA CAATACAGAG 
TTCTTCAAGA AGCTTGATCC TATGGAATTC TTTTCTGGGC ACTCATCACT TCATGCAGAG 
AATCAGCAGA AGGATTGCTC TCTGTCTGCT GGACCCTCAA ACGGAACTAA TCTGGCGCTG 
TCAAATGTTG ATGTTGAAGC AGCTATTAGA CAGGCAGAAG ATGAAGCTGA CTATATGGCT 
CTCAAGAGGC TGGAGCAGGA AGAGGCCGTG GACAATCAAG AGTTCAGCGA GGAGGCTGCT 
GGAAGACTTG AGGAGGATGA TCTTGTAAAC GAGGATGACA CTAAACCTGA TGAACACACC 
AATGAAGAGC ATAAATATCA GTGTTCTGAT TTGGTCAAGG ACAAGCATGT TGCTTTATCT 
ATAAATCAAT TAGATGAAGA AAAGGCTATA ACTTTGGCTG GAGGAGATGG AGATATTGAC 
ATGCTTGCTG ATGTTAAGCA GATGGCTGCT GCAGCTGCTG CAGCAGGTCA AGCAAGTTCG 
TCTTTTGAAA ATCAGCTCCG GCCAATTGAT AGATATGCCA TGCGGTTTTT GGAACTCTGG 
GATCCAATAA TTGATAAAGC TGCTATAAAC TATCAGGTCA ATGTTGAAGA GGAAGAATGG 
GAACTTGAAC GCATTGAAAA ACTCAAAGAA GATTTAGAAG CAGAAATTGA TGAAGACCAG 
GAACCACTTT CTTATGAGTC ATGGGATGTT GATTTTGCTA CTACAGCATA TCGGCAACAT 
GTTGAGGCTC TAGCTCAAAA ACAGTTGTTT GAGGAACAAG AAAGACAAGC CCGTGAAGCA 
GCAAAAGAGT TGGAGGAAAA GAATGATAAT AGCACTCAAC GAAAAAAGTC AAAAAAGAAC 
AAGAAGAAGG CAGCGAAGTT CAAATCTTTG AAGAAAGGAC GTTTATCATC TGAGTCAGAG 
GTTATGGTTG AGGAAACATC AGTAGATACT ATGAGCATTG ATGACAATGC ACCCTCACCA 
GAGCTAATGA GCGATGAATC AGCACACCAT CATTCTAACA AGCATAAGAG GATAATGTCA 
ACCAATGAGG AAGAGAACAG CAATAGCAGA AGCCTGAAGA AGCTCAAGAA AGCCCCTAAA 
TCGAGTTTCA GTTCCGAGGC CTTATCACCT AAGCATTTTC TTGAAGGCAA GCAGCTTAAG 
TTGAAAGATG AACTTAATGA TTCTGATCCC AAATCGGGGG CAAGAATTAA GAGTGATGGC 
CGAATTTCTG TCCCATGCAT GCCAGTAAAG CGTGTCATGG TAATCAAGCC TGAGAGATTG 
AAGAAGAAGA GCCTATGGTC TCGCGATTGT GCTTCAGACT CGTGGACAAC TGAGGAAGAT 
GCTGTTCTTT GTGCAACTGT AAATGAGTAC GGCCCTCTTT GGGAACTGGC TAGTGATTCT 
CTTCATTCTG TACCAGGTGG TGCATTTTAT AGAGGAAGAT ACCGTCACCC TGTGCATTGC 
TGTGAGAGAT TCCGGGAATT AGTCTGCAAG CACATCTTAT CAGCAACAGA CAATTCAAAC 
AGTGAGAAGG TTCCTTCTGG GACTGGGAAG GCTATCTTGA AAGTATCTGA GGATCAAACT 
CAGATGTTGC TCAATGTAAT CAGCGAGCTT CCTAACAATG AATTGCTTCT CCAGAAACAT 
TTCATGGCTG TTCTTTCATC TGTTTGGAGA TCGAAGTCTG CCTGTGAGTC CCACTGTGTT 
ATGAGTTACT CTAACACACT CCAGAAACCT GGCAGACTCA GCGAGAACTG GTCCATGACA 
AACTTCAGAC CAAACTTTAA TCTAGTAAGG ACAGCCCTCG CAGATGCTCA GGTTCAATGT 
CCAAGAATGG TAGTTCCAAC AAGCAATCAT GAGTCCCGCC GTAACTTTTT GGAATTGGAA 
TTGGATTTCT TGACAGATCG AGATGATTAT GAGGCGGACT TTCCATCTGT TGTTAACGTG 
TCCATACTAG AACCGGAACC ATTAAAACAT GCTGTGGAGC CAGTGGAGCA CTCACTACTG 
TCCACACTTT CTTATAGACA TGCTGAGAAC AGATTCAGGA TGGTATCAGA AACTTGTTTT 
GAAGGGGAAG GTTCTCACTG GGCCTCATCT GCTTTCCACA CATACGATGC CGGTCGGCAT 
AAATCTGGTC CTAAGTCCAT AGGAAAGCAC AAAGCATCAT CAGAATCTGG AAGGCCTCCC 
AAATCCAAAA TTCAAAGGAC CACTGAACCA CAGGAAGTGC CGGTTACAAA TAATTTTCAT 
AGGATTCCGG GGCAACTTCT GCATAACTCA GCAGAGTTCC ACATTACCCA GTCCCTATCT 
GACCTTGGCA TCAGTGATTC TGAATTCACC TACTTTGATA ATCTTCCTCA AGAGGCTGAA 
ACAGAGTTTG TTCCGTACCA GTATGACTCT GATGTCCTTT CTGGCATTGA GGAGTTGGAT 
CCTCTTACAG ATTTCACAGA CATCGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025878605.1
CDS	406..6555
Translation	MASKGPRSKLDHETRARRQKALEAPRGEPRRTKTHWDHLLGEMAWLAKEFDAERKWKLSMAKRIAQRANKGVVDQATKDERKQKEEEVRLRKVALNISKDVKKFWTKIEKLVFYKNQLELEERKKKALDKQLDFLLGQTERYSTMLAENLVDVRLQNQENDSLQTNQRSQQELAQENINASSPTDVDNVEIDDDYNSSLGEEPKDDEHTIDEDEAQITEAERNEELAALQAEADLPLDDILKLYTKNKVQAFSVSRESSPDGRDVFSDSDSKDLIKDPLNQANGCNDESDHTSSDEGISSEEADDYQSYSEFVKKNTVKCNGNISSVDAKDDEDYVANDEGKDDEATLSEEEELAKKEDCDPLDEVKLLQKESEIPLEELLARYQKDGYADDDTTELENSPALSVEDVNANMPVDDESADTVEVNRDLSADTMKLTRDQSAETVKVNNDQSAEIVEGNNDTFEDHESAGMLGPEHVSGSVLQLETSEPIVQENTAKEGDVTDTKAMANGDNSDVIADAAAAARSAQPTGNTFSTTNVRTKFPFLLKHSLREYQHIGLDWLVAMYEKRLNGILADEMGLGKTIMTISLLAHLACEKGIWGPHLIVVPTSVMLNWETEFLKWCPAFKILTYFGSAKERKQKRQGWMKPNYFHVCITTYRLVIQDSKVFKRKKWKYLILDEAHLIKNWKSQRWQTLLNFNSKRRILLTGTPLQNDLMELWSLMHFLMPHVFQSHQEFKDWFCNPISGMVEGQDKVNKEVIDRLHNVLRPFILRRLKRDVEKQLPQKHEHVIYCRLSRRQRNLYEDFIASSETQATLASGNYFGMISIIMQLRKVCNHPDLFEGRPIISSFDMAGINMQISSSVGMVLDKGPFSQVDLSDMNFVFTQNEYNMTSWEEDEVAAIFSPSITLRGSGISRSTNDGQRSNGTNIFEEIHNSLWEERIKEANERAASIAWWNRVRCQKRPVYGTNIREVLTIKHPVSDILEKKKNPLCHMEFSSSLANLVLSSVDRFKTMLDFIESFTFAIPAARAPAPLFWCNKGKSPVLIEPAYREKCMNEFSPVFSPIRPAIVRRQVYFPDRRLIQFDCGKLQELAILLRRLKSEGHRALIFTQMTKMLDILEEFINLYGYTYLRLDGSTQPEERQTLMQRFNTNPKFFLFILSTRSGGVGINLVGADTVIFYDSDWNPAMDQQAQDRCHRIGQTREVHIYRLISESTIEENILKKANQKRALDDLVIQRGSYNTEFFKKLDPMEFFSGHSSLHAENQQKDCSLSAGPSNGTNLALSNVDVEAAIRQAEDEADYMALKRLEQEEAVDNQEFSEEAAGRLEEDDLVNEDDTKPDEHTNEEHKYQCSDLVKDKHVALSINQLDEEKAITLAGGDGDIDMLADVKQMAAAAAAAGQASSSFENQLRPIDRYAMRFLELWDPIIDKAAINYQVNVEEEEWELERIEKLKEDLEAEIDEDQEPLSYESWDVDFATTAYRQHVEALAQKQLFEEQERQAREAAKELEEKNDNSTQRKKSKKNKKKAAKFKSLKKGRLSSESEVMVEETSVDTMSIDDNAPSPELMSDESAHHHSNKHKRIMSTNEEENSNSRSLKKLKKAPKSSFSSEALSPKHFLEGKQLKLKDELNDSDPKSGARIKSDGRISVPCMPVKRVMVIKPERLKKKSLWSRDCASDSWTTEEDAVLCATVNEYGPLWELASDSLHSVPGGAFYRGRYRHPVHCCERFRELVCKHILSATDNSNSEKVPSGTGKAILKVSEDQTQMLLNVISELPNNELLLQKHFMAVLSSVWRSKSACESHCVMSYSNTLQKPGRLSENWSMTNFRPNFNLVRTALADAQVQCPRMVVPTSNHESRRNFLELELDFLTDRDDYEADFPSVVNVSILEPEPLKHAVEPVEHSLLSTLSYRHAENRFRMVSETCFEGEGSHWASSAFHTYDAGRHKSGPKSIGKHKASSESGRPPKSKIQRTTEPQEVPVTNNFHRIPGQLLHNSAEFHITQSLSDLGISDSEFTYFDNLPQEAETEFVPYQYDSDVLSGIEELDPLTDFTDIG

Target ORF information:

RefSeq Version	XM_026022820.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026022820.1

ORF Insert Sequence:

ATGGCATCAA AAGGTCCTCG ATCAAAGTTA GATCATGAGA CAAGAGCCAG ACGCCAGAAG 
GCTCTTGAAG CCCCAAGGGG GGAGCCTCGA AGGACGAAAA CTCACTGGGA CCATCTTCTT 
GGAGAAATGG CTTGGCTGGC AAAGGAGTTT GATGCTGAGA GAAAATGGAA GCTGTCCATG 
GCTAAAAGGA TTGCTCAAAG AGCTAATAAG GGTGTAGTTG ACCAAGCAAC AAAGGACGAG 
AGAAAACAGA AGGAGGAAGA AGTTCGCCTG AGAAAAGTTG CCCTAAATAT TTCTAAGGAC 
GTGAAGAAGT TCTGGACCAA AATAGAGAAG CTGGTTTTTT ATAAGAACCA ACTGGAGCTT 
GAGGAAAGGA AAAAAAAGGC CCTAGACAAG CAACTTGATT TCCTCTTAGG ACAGACTGAA 
AGATATTCAA CAATGCTGGC AGAAAATCTT GTTGATGTGC GCCTTCAAAA TCAAGAAAAT 
GACTCTTTAC AGACTAATCA AAGATCCCAG CAGGAACTAG CACAAGAGAA CATAAATGCA 
TCAAGTCCGA CTGATGTTGA CAATGTGGAA ATCGATGATG ATTACAACAG TTCCTTGGGT 
GAAGAGCCAA AAGATGATGA ACACACAATC GATGAGGATG AAGCTCAAAT TACGGAGGCT 
GAGCGCAACG AGGAATTGGC TGCTTTGCAG GCAGAAGCTG ATCTACCACT GGATGATATT 
CTCAAGTTGT ACACTAAAAA TAAAGTTCAA GCTTTCTCAG TTAGCAGGGA AAGCAGTCCA 
GATGGCAGAG ACGTTTTTAG TGACTCAGAT TCAAAGGATT TGATTAAAGA TCCTTTGAAC 
CAGGCTAATG GATGCAACGA TGAATCTGAT CATACTTCAA GTGATGAAGG CATTTCTTCT 
GAGGAAGCAG ATGATTACCA GTCTTACTCT GAGTTTGTGA AAAAGAACAC TGTGAAATGC 
AATGGCAATA TCTCATCTGT TGATGCAAAG GACGACGAAG ATTATGTTGC TAATGATGAA 
GGAAAGGATG ATGAAGCTAC TTTGTCTGAA GAGGAAGAGT TGGCGAAGAA AGAAGACTGT 
GATCCTCTAG ATGAGGTTAA GTTACTGCAG AAAGAGAGTG AGATCCCACT TGAAGAACTT 
CTTGCAAGAT ACCAAAAGGA TGGCTATGCA GATGATGACA CAACAGAATT GGAAAATTCA 
CCTGCACTAT CAGTTGAGGA TGTCAATGCC AACATGCCTG TGGATGATGA GTCTGCAGAC 
ACCGTGGAGG TAAACAGGGA TCTTTCTGCA GATACCATGA AACTGACCAG AGATCAGTCT 
GCAGAGACCG TAAAAGTGAA TAATGACCAG TCTGCAGAGA TTGTGGAGGG CAACAATGAC 
ACATTTGAAG ATCATGAATC TGCAGGCATG CTGGGACCTG AGCATGTCAG TGGTAGTGTT 
CTACAACTAG AAACATCAGA GCCTATTGTA CAGGAAAATA CTGCTAAAGA GGGTGATGTA 
ACTGATACTA AAGCGATGGC CAATGGAGAT AATAGTGATG TGATTGCTGA TGCTGCAGCT 
GCTGCAAGAT CAGCACAACC GACTGGTAAC ACGTTCTCAA CAACAAATGT GCGGACAAAA 
TTCCCTTTCC TTCTTAAGCA TTCTCTACGA GAATATCAGC ACATTGGACT GGACTGGCTT 
GTTGCTATGT ATGAAAAGAG GCTGAATGGA ATTCTAGCAG ATGAAATGGG TTTGGGGAAG 
ACAATTATGA CCATCTCCTT GCTAGCACAC CTTGCATGTG AGAAGGGAAT ATGGGGTCCA 
CATCTTATTG TAGTGCCAAC TAGTGTTATG CTAAATTGGG AGACTGAATT TCTCAAATGG 
TGTCCTGCGT TTAAAATACT GACATATTTT GGAAGTGCAA AAGAGAGAAA ACAGAAACGT 
CAAGGTTGGA TGAAACCAAA TTACTTCCAT GTATGCATCA CTACATACAG GCTTGTTATT 
CAGGACTCCA AAGTGTTTAA GCGAAAAAAA TGGAAGTATC TTATTCTTGA TGAGGCTCAT 
TTGATAAAGA ACTGGAAATC ACAAAGATGG CAGACTTTAC TAAATTTTAA TTCGAAGCGA 
CGCATTCTTT TAACTGGAAC TCCTCTACAA AATGACCTCA TGGAACTTTG GTCTCTCATG 
CACTTTTTGA TGCCTCATGT ATTTCAGTCA CATCAAGAGT TCAAGGACTG GTTCTGCAAT 
CCAATTTCAG GCATGGTAGA GGGCCAAGAC AAGGTAAACA AAGAAGTGAT AGATCGGTTG 
CACAACGTCC TTCGTCCGTT TATACTTCGT CGATTGAAAC GGGATGTGGA GAAACAGTTG 
CCACAGAAGC ATGAGCATGT CATATATTGC CGACTTTCCA GAAGGCAAAG GAACTTGTAT 
GAAGATTTCA TTGCAAGCTC AGAGACACAG GCAACATTGG CAAGTGGAAA TTATTTTGGC 
ATGATTAGCA TCATTATGCA GCTTAGAAAG GTTTGTAACC ATCCAGATCT TTTTGAAGGC 
CGTCCAATTA TCAGCTCTTT TGACATGGCA GGGATCAACA TGCAAATCAG CTCTTCTGTT 
GGCATGGTCC TTGATAAAGG CCCATTTTCT CAGGTTGACC TGTCAGATAT GAATTTTGTA 
TTTACTCAAA ATGAATATAA TATGACTTCT TGGGAGGAAG ATGAGGTTGC TGCCATTTTT 
TCTCCAAGCA TAACCCTCAG GGGTTCTGGC ATTTCTCGCT CTACTAATGA TGGTCAGAGA 
AGCAATGGGA CAAATATTTT TGAAGAGATT CACAATTCTT TGTGGGAGGA GAGAATAAAA 
GAAGCTAATG AAAGAGCAGC TTCCATTGCA TGGTGGAATA GGGTAAGATG CCAGAAGAGG 
CCTGTTTATG GCACAAATAT TCGGGAGGTT TTGACCATAA AGCATCCTGT GTCTGATATT 
CTCGAGAAGA AGAAGAACCC TTTATGCCAC ATGGAATTTT CATCAAGCCT AGCAAATCTT 
GTTCTATCAT CGGTGGATCG CTTCAAGACA ATGCTTGATT TTATTGAGTC ATTTACTTTT 
GCAATTCCTG CTGCACGAGC GCCTGCACCA TTATTCTGGT GCAACAAAGG GAAATCTCCT 
GTTCTTATTG AACCAGCATA CAGAGAGAAA TGCATGAATG AGTTTTCTCC TGTTTTCTCT 
CCTATAAGGC CTGCGATTGT TCGCCGTCAA GTGTACTTTC CTGACAGGCG TTTGATCCAG 
TTTGACTGTG GAAAGTTGCA GGAACTTGCT ATTTTGTTGA GGCGTTTGAA GTCAGAAGGA 
CACAGGGCCT TGATATTTAC TCAGATGACT AAGATGCTTG ATATACTGGA GGAGTTCATT 
AATCTATATG GGTATACGTA TTTGCGGTTA GATGGATCAA CCCAGCCAGA AGAGAGGCAG 
ACACTGATGC AGAGGTTTAA TACTAACCCA AAGTTTTTTC TTTTTATCTT ATCCACTCGT 
AGTGGTGGTG TGGGAATCAA CCTAGTAGGT GCAGACACTG TCATTTTCTA TGATAGTGAC 
TGGAATCCTG CAATGGACCA ACAGGCCCAA GACAGATGCC ACAGGATAGG TCAAACTCGT 
GAAGTTCACA TCTATAGACT GATCAGTGAG AGCACTATAG AGGAGAATAT ACTCAAGAAA 
GCCAATCAGA AGCGAGCTCT TGATGATCTA GTGATACAAC GCGGTAGCTA CAATACAGAG 
TTCTTCAAGA AGCTTGATCC TATGGAATTC TTTTCTGGGC ACTCATCACT TCATGCAGAG 
AATCAGCAGA AGGATTGCTC TCTGTCTGCT GGACCCTCAA ACGGAACTAA TCTGGCGCTG 
TCAAATGTTG ATGTTGAAGC AGCTATTAGA CAGGCAGAAG ATGAAGCTGA CTATATGGCT 
CTCAAGAGGC TGGAGCAGGA AGAGGCCGTG GACAATCAAG AGTTCAGCGA GGAGGCTGCT 
GGAAGACTTG AGGAGGATGA TCTTGTAAAC GAGGATGACA CTAAACCTGA TGAACACACC 
AATGAAGAGC ATAAATATCA GTGTTCTGAT TTGGTCAAGG ACAAGCATGT TGCTTTATCT 
ATAAATCAAT TAGATGAAGA AAAGGCTATA ACTTTGGCTG GAGGAGATGG AGATATTGAC 
ATGCTTGCTG ATGTTAAGCA GATGGCTGCT GCAGCTGCTG CAGCAGGTCA AGCAAGTTCG 
TCTTTTGAAA ATCAGCTCCG GCCAATTGAT AGATATGCCA TGCGGTTTTT GGAACTCTGG 
GATCCAATAA TTGATAAAGC TGCTATAAAC TATCAGGTCA ATGTTGAAGA GGAAGAATGG 
GAACTTGAAC GCATTGAAAA ACTCAAAGAA GATTTAGAAG CAGAAATTGA TGAAGACCAG 
GAACCACTTT CTTATGAGTC ATGGGATGTT GATTTTGCTA CTACAGCATA TCGGCAACAT 
GTTGAGGCTC TAGCTCAAAA ACAGTTGTTT GAGGAACAAG AAAGACAAGC CCGTGAAGCA 
GCAAAAGAGT TGGAGGAAAA GAATGATAAT AGCACTCAAC GAAAAAAGTC AAAAAAGAAC 
AAGAAGAAGG CAGCGAAGTT CAAATCTTTG AAGAAAGGAC GTTTATCATC TGAGTCAGAG 
GTTATGGTTG AGGAAACATC AGTAGATACT ATGAGCATTG ATGACAATGC ACCCTCACCA 
GAGCTAATGA GCGATGAATC AGCACACCAT CATTCTAACA AGCATAAGAG GATAATGTCA 
ACCAATGAGG AAGAGAACAG CAATAGCAGA AGCCTGAAGA AGCTCAAGAA AGCCCCTAAA 
TCGAGTTTCA GTTCCGAGGC CTTATCACCT AAGCATTTTC TTGAAGGCAA GCAGCTTAAG 
TTGAAAGATG AACTTAATGA TTCTGATCCC AAATCGGGGG CAAGAATTAA GAGTGATGGC 
CGAATTTCTG TCCCATGCAT GCCAGTAAAG CGTGTCATGG TAATCAAGCC TGAGAGATTG 
AAGAAGAAGA GCCTATGGTC TCGCGATTGT GCTTCAGACT CGTGGACAAC TGAGGAAGAT 
GCTGTTCTTT GTGCAACTGT AAATGAGTAC GGCCCTCTTT GGGAACTGGC TAGTGATTCT 
CTTCATTCTG TACCAGGTGG TGCATTTTAT AGAGGAAGAT ACCGTCACCC TGTGCATTGC 
TGTGAGAGAT TCCGGGAATT AGTCTGCAAG CACATCTTAT CAGCAACAGA CAATTCAAAC 
AGTGAGAAGG TTCCTTCTGG GACTGGGAAG GCTATCTTGA AAGTATCTGA GGATCAAACT 
CAGATGTTGC TCAATGTAAT CAGCGAGCTT CCTAACAATG AATTGCTTCT CCAGAAACAT 
TTCATGGCTG TTCTTTCATC TGTTTGGAGA TCGAAGTCTG CCTGTGAGTC CCACTGTGTT 
ATGAGTTACT CTAACACACT CCAGAAACCT GGCAGACTCA GCGAGAACTG GTCCATGACA 
AACTTCAGAC CAAACTTTAA TCTAGTAAGG ACAGCCCTCG CAGATGCTCA GGTTCAATGT 
CCAAGAATGG TAGTTCCAAC AAGCAATCAT GAGTCCCGCC GTAACTTTTT GGAATTGGAA 
TTGGATTTCT TGACAGATCG AGATGATTAT GAGGCGGACT TTCCATCTGT TGTTAACGTG 
TCCATACTAG AACCGGAACC ATTAAAACAT GCTGTGGAGC CAGTGGAGCA CTCACTACTG 
TCCACACTTT CTTATAGACA TGCTGAGAAC AGATTCAGGA TGGTATCAGA AACTTGTTTT 
GAAGGGGAAG GTTCTCACTG GGCCTCATCT GCTTTCCACA CATACGATGC CGGTCGGCAT 
AAATCTGGTC CTAAGTCCAT AGGAAAGCAC AAAGCATCAT CAGAATCTGG AAGGCCTCCC 
AAATCCAAAA TTCAAAGGAC CACTGAACCA CAGGAAGTGC CGGTTACAAA TAATTTTCAT 
AGGATTCCGG GGCAACTTCT GCATAACTCA GCAGAGTTCC ACATTACCCA GTCCCTATCT 
GACCTTGGCA TCAGTGATTC TGAATTCACC TACTTTGATA ATCTTCCTCA AGAGGCTGAA 
ACAGAGTTTG TTCCGTACCA GTATGACTCT GATGTCCTTT CTGGCATTGA GGAGTTGGAT 
CCTCTTACAG ATTTCACAGA CATCGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4330371 ( XM_026022821.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_025878606.1 Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X2, mRNA.

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CloneID	OOa45722
Clone ID Related Accession (Same CDS sequence)	XM_026022820.1 , XM_026022821.1
Accession Version	XM_026022821.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029257.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCAA AAGGTCCTCG ATCAAAGTTA GATCATGAGA CAAGAGCCAG ACGCCAGAAG 
GCTCTTGAAG CCCCAAGGGG GGAGCCTCGA AGGACGAAAA CTCACTGGGA CCATCTTCTT 
GGAGAAATGG CTTGGCTGGC AAAGGAGTTT GATGCTGAGA GAAAATGGAA GCTGTCCATG 
GCTAAAAGGA TTGCTCAAAG AGCTAATAAG GGTGTAGTTG ACCAAGCAAC AAAGGACGAG 
AGAAAACAGA AGGAGGAAGA AGTTCGCCTG AGAAAAGTTG CCCTAAATAT TTCTAAGGAC 
GTGAAGAAGT TCTGGACCAA AATAGAGAAG CTGGTTTTTT ATAAGAACCA ACTGGAGCTT 
GAGGAAAGGA AAAAAAAGGC CCTAGACAAG CAACTTGATT TCCTCTTAGG ACAGACTGAA 
AGATATTCAA CAATGCTGGC AGAAAATCTT GTTGATGTGC GCCTTCAAAA TCAAGAAAAT 
GACTCTTTAC AGACTAATCA AAGATCCCAG CAGGAACTAG CACAAGAGAA CATAAATGCA 
TCAAGTCCGA CTGATGTTGA CAATGTGGAA ATCGATGATG ATTACAACAG TTCCTTGGGT 
GAAGAGCCAA AAGATGATGA ACACACAATC GATGAGGATG AAGCTCAAAT TACGGAGGCT 
GAGCGCAACG AGGAATTGGC TGCTTTGCAG GCAGAAGCTG ATCTACCACT GGATGATATT 
CTCAAGTTGT ACACTAAAAA TAAAGTTCAA GCTTTCTCAG TTAGCAGGGA AAGCAGTCCA 
GATGGCAGAG ACGTTTTTAG TGACTCAGAT TCAAAGGATT TGATTAAAGA TCCTTTGAAC 
CAGGCTAATG GATGCAACGA TGAATCTGAT CATACTTCAA GTGATGAAGG CATTTCTTCT 
GAGGAAGCAG ATGATTACCA GTCTTACTCT GAGTTTGTGA AAAAGAACAC TGTGAAATGC 
AATGGCAATA TCTCATCTGT TGATGCAAAG GACGACGAAG ATTATGTTGC TAATGATGAA 
GGAAAGGATG ATGAAGCTAC TTTGTCTGAA GAGGAAGAGT TGGCGAAGAA AGAAGACTGT 
GATCCTCTAG ATGAGGTTAA GTTACTGCAG AAAGAGAGTG AGATCCCACT TGAAGAACTT 
CTTGCAAGAT ACCAAAAGGA TGGCTATGCA GATGATGACA CAACAGAATT GGAAAATTCA 
CCTGCACTAT CAGTTGAGGA TGTCAATGCC AACATGCCTG TGGATGATGA GTCTGCAGAC 
ACCGTGGAGG TAAACAGGGA TCTTTCTGCA GATACCATGA AACTGACCAG AGATCAGTCT 
GCAGAGACCG TAAAAGTGAA TAATGACCAG TCTGCAGAGA TTGTGGAGGG CAACAATGAC 
ACATTTGAAG ATCATGAATC TGCAGGCATG CTGGGACCTG AGCATGTCAG TGGTAGTGTT 
CTACAACTAG AAACATCAGA GCCTATTGTA CAGGAAAATA CTGCTAAAGA GGGTGATGTA 
ACTGATACTA AAGCGATGGC CAATGGAGAT AATAGTGATG TGATTGCTGA TGCTGCAGCT 
GCTGCAAGAT CAGCACAACC GACTGGTAAC ACGTTCTCAA CAACAAATGT GCGGACAAAA 
TTCCCTTTCC TTCTTAAGCA TTCTCTACGA GAATATCAGC ACATTGGACT GGACTGGCTT 
GTTGCTATGT ATGAAAAGAG GCTGAATGGA ATTCTAGCAG ATGAAATGGG TTTGGGGAAG 
ACAATTATGA CCATCTCCTT GCTAGCACAC CTTGCATGTG AGAAGGGAAT ATGGGGTCCA 
CATCTTATTG TAGTGCCAAC TAGTGTTATG CTAAATTGGG AGACTGAATT TCTCAAATGG 
TGTCCTGCGT TTAAAATACT GACATATTTT GGAAGTGCAA AAGAGAGAAA ACAGAAACGT 
CAAGGTTGGA TGAAACCAAA TTACTTCCAT GTATGCATCA CTACATACAG GCTTGTTATT 
CAGGACTCCA AAGTGTTTAA GCGAAAAAAA TGGAAGTATC TTATTCTTGA TGAGGCTCAT 
TTGATAAAGA ACTGGAAATC ACAAAGATGG CAGACTTTAC TAAATTTTAA TTCGAAGCGA 
CGCATTCTTT TAACTGGAAC TCCTCTACAA AATGACCTCA TGGAACTTTG GTCTCTCATG 
CACTTTTTGA TGCCTCATGT ATTTCAGTCA CATCAAGAGT TCAAGGACTG GTTCTGCAAT 
CCAATTTCAG GCATGGTAGA GGGCCAAGAC AAGGTAAACA AAGAAGTGAT AGATCGGTTG 
CACAACGTCC TTCGTCCGTT TATACTTCGT CGATTGAAAC GGGATGTGGA GAAACAGTTG 
CCACAGAAGC ATGAGCATGT CATATATTGC CGACTTTCCA GAAGGCAAAG GAACTTGTAT 
GAAGATTTCA TTGCAAGCTC AGAGACACAG GCAACATTGG CAAGTGGAAA TTATTTTGGC 
ATGATTAGCA TCATTATGCA GCTTAGAAAG GTTTGTAACC ATCCAGATCT TTTTGAAGGC 
CGTCCAATTA TCAGCTCTTT TGACATGGCA GGGATCAACA TGCAAATCAG CTCTTCTGTT 
GGCATGGTCC TTGATAAAGG CCCATTTTCT CAGGTTGACC TGTCAGATAT GAATTTTGTA 
TTTACTCAAA ATGAATATAA TATGACTTCT TGGGAGGAAG ATGAGGTTGC TGCCATTTTT 
TCTCCAAGCA TAACCCTCAG GGGTTCTGGC ATTTCTCGCT CTACTAATGA TGGTCAGAGA 
AGCAATGGGA CAAATATTTT TGAAGAGATT CACAATTCTT TGTGGGAGGA GAGAATAAAA 
GAAGCTAATG AAAGAGCAGC TTCCATTGCA TGGTGGAATA GGGTAAGATG CCAGAAGAGG 
CCTGTTTATG GCACAAATAT TCGGGAGGTT TTGACCATAA AGCATCCTGT GTCTGATATT 
CTCGAGAAGA AGAAGAACCC TTTATGCCAC ATGGAATTTT CATCAAGCCT AGCAAATCTT 
GTTCTATCAT CGGTGGATCG CTTCAAGACA ATGCTTGATT TTATTGAGTC ATTTACTTTT 
GCAATTCCTG CTGCACGAGC GCCTGCACCA TTATTCTGGT GCAACAAAGG GAAATCTCCT 
GTTCTTATTG AACCAGCATA CAGAGAGAAA TGCATGAATG AGTTTTCTCC TGTTTTCTCT 
CCTATAAGGC CTGCGATTGT TCGCCGTCAA GTGTACTTTC CTGACAGGCG TTTGATCCAG 
TTTGACTGTG GAAAGTTGCA GGAACTTGCT ATTTTGTTGA GGCGTTTGAA GTCAGAAGGA 
CACAGGGCCT TGATATTTAC TCAGATGACT AAGATGCTTG ATATACTGGA GGAGTTCATT 
AATCTATATG GGTATACGTA TTTGCGGTTA GATGGATCAA CCCAGCCAGA AGAGAGGCAG 
ACACTGATGC AGAGGTTTAA TACTAACCCA AAGTTTTTTC TTTTTATCTT ATCCACTCGT 
AGTGGTGGTG TGGGAATCAA CCTAGTAGGT GCAGACACTG TCATTTTCTA TGATAGTGAC 
TGGAATCCTG CAATGGACCA ACAGGCCCAA GACAGATGCC ACAGGATAGG TCAAACTCGT 
GAAGTTCACA TCTATAGACT GATCAGTGAG AGCACTATAG AGGAGAATAT ACTCAAGAAA 
GCCAATCAGA AGCGAGCTCT TGATGATCTA GTGATACAAC GCGGTAGCTA CAATACAGAG 
TTCTTCAAGA AGCTTGATCC TATGGAATTC TTTTCTGGGC ACTCATCACT TCATGCAGAG 
AATCAGCAGA AGGATTGCTC TCTGTCTGCT GGACCCTCAA ACGGAACTAA TCTGGCGCTG 
TCAAATGTTG ATGTTGAAGC AGCTATTAGA CAGGCAGAAG ATGAAGCTGA CTATATGGCT 
CTCAAGAGGC TGGAGCAGGA AGAGGCCGTG GACAATCAAG AGTTCAGCGA GGAGGCTGCT 
GGAAGACTTG AGGAGGATGA TCTTGTAAAC GAGGATGACA CTAAACCTGA TGAACACACC 
AATGAAGAGC ATAAATATCA GTGTTCTGAT TTGGTCAAGG ACAAGCATGT TGCTTTATCT 
ATAAATCAAT TAGATGAAGA AAAGGCTATA ACTTTGGCTG GAGGAGATGG AGATATTGAC 
ATGCTTGCTG ATGTTAAGCA GATGGCTGCT GCAGCTGCTG CAGCAGGTCA AGCAAGTTCG 
TCTTTTGAAA ATCAGCTCCG GCCAATTGAT AGATATGCCA TGCGGTTTTT GGAACTCTGG 
GATCCAATAA TTGATAAAGC TGCTATAAAC TATCAGGTCA ATGTTGAAGA GGAAGAATGG 
GAACTTGAAC GCATTGAAAA ACTCAAAGAA GATTTAGAAG CAGAAATTGA TGAAGACCAG 
GAACCACTTT CTTATGAGTC ATGGGATGTT GATTTTGCTA CTACAGCATA TCGGCAACAT 
GTTGAGGCTC TAGCTCAAAA ACAGTTGTTT GAGGAACAAG AAAGACAAGC CCGTGAAGCA 
GCAAAAGAGT TGGAGGAAAA GAATGATAAT AGCACTCAAC GAAAAAAGTC AAAAAAGAAC 
AAGAAGAAGG CAGCGAAGTT CAAATCTTTG AAGAAAGGAC GTTTATCATC TGAGTCAGAG 
GTTATGGTTG AGGAAACATC AGTAGATACT ATGAGCATTG ATGACAATGC ACCCTCACCA 
GAGCTAATGA GCGATGAATC AGCACACCAT CATTCTAACA AGCATAAGAG GATAATGTCA 
ACCAATGAGG AAGAGAACAG CAATAGCAGA AGCCTGAAGA AGCTCAAGAA AGCCCCTAAA 
TCGAGTTTCA GTTCCGAGGC CTTATCACCT AAGCATTTTC TTGAAGGCAA GCAGCTTAAG 
TTGAAAGATG AACTTAATGA TTCTGATCCC AAATCGGGGG CAAGAATTAA GAGTGATGGC 
CGAATTTCTG TCCCATGCAT GCCAGTAAAG CGTGTCATGG TAATCAAGCC TGAGAGATTG 
AAGAAGAAGA GCCTATGGTC TCGCGATTGT GCTTCAGACT CGTGGACAAC TGAGGAAGAT 
GCTGTTCTTT GTGCAACTGT AAATGAGTAC GGCCCTCTTT GGGAACTGGC TAGTGATTCT 
CTTCATTCTG TACCAGGTGG TGCATTTTAT AGAGGAAGAT ACCGTCACCC TGTGCATTGC 
TGTGAGAGAT TCCGGGAATT AGTCTGCAAG CACATCTTAT CAGCAACAGA CAATTCAAAC 
AGTGAGAAGG TTCCTTCTGG GACTGGGAAG GCTATCTTGA AAGTATCTGA GGATCAAACT 
CAGATGTTGC TCAATGTAAT CAGCGAGCTT CCTAACAATG AATTGCTTCT CCAGAAACAT 
TTCATGGCTG TTCTTTCATC TGTTTGGAGA TCGAAGTCTG CCTGTGAGTC CCACTGTGTT 
ATGAGTTACT CTAACACACT CCAGAAACCT GGCAGACTCA GCGAGAACTG GTCCATGACA 
AACTTCAGAC CAAACTTTAA TCTAGTAAGG ACAGCCCTCG CAGATGCTCA GGTTCAATGT 
CCAAGAATGG TAGTTCCAAC AAGCAATCAT GAGTCCCGCC GTAACTTTTT GGAATTGGAA 
TTGGATTTCT TGACAGATCG AGATGATTAT GAGGCGGACT TTCCATCTGT TGTTAACGTG 
TCCATACTAG AACCGGAACC ATTAAAACAT GCTGTGGAGC CAGTGGAGCA CTCACTACTG 
TCCACACTTT CTTATAGACA TGCTGAGAAC AGATTCAGGA TGGTATCAGA AACTTGTTTT 
GAAGGGGAAG GTTCTCACTG GGCCTCATCT GCTTTCCACA CATACGATGC CGGTCGGCAT 
AAATCTGGTC CTAAGTCCAT AGGAAAGCAC AAAGCATCAT CAGAATCTGG AAGGCCTCCC 
AAATCCAAAA TTCAAAGGAC CACTGAACCA CAGGAAGTGC CGGTTACAAA TAATTTTCAT 
AGGATTCCGG GGCAACTTCT GCATAACTCA GCAGAGTTCC ACATTACCCA GTCCCTATCT 
GACCTTGGCA TCAGTGATTC TGAATTCACC TACTTTGATA ATCTTCCTCA AGAGGCTGAA 
ACAGAGTTTG TTCCGTACCA GTATGACTCT GATGTCCTTT CTGGCATTGA GGAGTTGGAT 
CCTCTTACAG ATTTCACAGA CATCGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025878606.1
CDS	449..6598
Translation	MASKGPRSKLDHETRARRQKALEAPRGEPRRTKTHWDHLLGEMAWLAKEFDAERKWKLSMAKRIAQRANKGVVDQATKDERKQKEEEVRLRKVALNISKDVKKFWTKIEKLVFYKNQLELEERKKKALDKQLDFLLGQTERYSTMLAENLVDVRLQNQENDSLQTNQRSQQELAQENINASSPTDVDNVEIDDDYNSSLGEEPKDDEHTIDEDEAQITEAERNEELAALQAEADLPLDDILKLYTKNKVQAFSVSRESSPDGRDVFSDSDSKDLIKDPLNQANGCNDESDHTSSDEGISSEEADDYQSYSEFVKKNTVKCNGNISSVDAKDDEDYVANDEGKDDEATLSEEEELAKKEDCDPLDEVKLLQKESEIPLEELLARYQKDGYADDDTTELENSPALSVEDVNANMPVDDESADTVEVNRDLSADTMKLTRDQSAETVKVNNDQSAEIVEGNNDTFEDHESAGMLGPEHVSGSVLQLETSEPIVQENTAKEGDVTDTKAMANGDNSDVIADAAAAARSAQPTGNTFSTTNVRTKFPFLLKHSLREYQHIGLDWLVAMYEKRLNGILADEMGLGKTIMTISLLAHLACEKGIWGPHLIVVPTSVMLNWETEFLKWCPAFKILTYFGSAKERKQKRQGWMKPNYFHVCITTYRLVIQDSKVFKRKKWKYLILDEAHLIKNWKSQRWQTLLNFNSKRRILLTGTPLQNDLMELWSLMHFLMPHVFQSHQEFKDWFCNPISGMVEGQDKVNKEVIDRLHNVLRPFILRRLKRDVEKQLPQKHEHVIYCRLSRRQRNLYEDFIASSETQATLASGNYFGMISIIMQLRKVCNHPDLFEGRPIISSFDMAGINMQISSSVGMVLDKGPFSQVDLSDMNFVFTQNEYNMTSWEEDEVAAIFSPSITLRGSGISRSTNDGQRSNGTNIFEEIHNSLWEERIKEANERAASIAWWNRVRCQKRPVYGTNIREVLTIKHPVSDILEKKKNPLCHMEFSSSLANLVLSSVDRFKTMLDFIESFTFAIPAARAPAPLFWCNKGKSPVLIEPAYREKCMNEFSPVFSPIRPAIVRRQVYFPDRRLIQFDCGKLQELAILLRRLKSEGHRALIFTQMTKMLDILEEFINLYGYTYLRLDGSTQPEERQTLMQRFNTNPKFFLFILSTRSGGVGINLVGADTVIFYDSDWNPAMDQQAQDRCHRIGQTREVHIYRLISESTIEENILKKANQKRALDDLVIQRGSYNTEFFKKLDPMEFFSGHSSLHAENQQKDCSLSAGPSNGTNLALSNVDVEAAIRQAEDEADYMALKRLEQEEAVDNQEFSEEAAGRLEEDDLVNEDDTKPDEHTNEEHKYQCSDLVKDKHVALSINQLDEEKAITLAGGDGDIDMLADVKQMAAAAAAAGQASSSFENQLRPIDRYAMRFLELWDPIIDKAAINYQVNVEEEEWELERIEKLKEDLEAEIDEDQEPLSYESWDVDFATTAYRQHVEALAQKQLFEEQERQAREAAKELEEKNDNSTQRKKSKKNKKKAAKFKSLKKGRLSSESEVMVEETSVDTMSIDDNAPSPELMSDESAHHHSNKHKRIMSTNEEENSNSRSLKKLKKAPKSSFSSEALSPKHFLEGKQLKLKDELNDSDPKSGARIKSDGRISVPCMPVKRVMVIKPERLKKKSLWSRDCASDSWTTEEDAVLCATVNEYGPLWELASDSLHSVPGGAFYRGRYRHPVHCCERFRELVCKHILSATDNSNSEKVPSGTGKAILKVSEDQTQMLLNVISELPNNELLLQKHFMAVLSSVWRSKSACESHCVMSYSNTLQKPGRLSENWSMTNFRPNFNLVRTALADAQVQCPRMVVPTSNHESRRNFLELELDFLTDRDDYEADFPSVVNVSILEPEPLKHAVEPVEHSLLSTLSYRHAENRFRMVSETCFEGEGSHWASSAFHTYDAGRHKSGPKSIGKHKASSESGRPPKSKIQRTTEPQEVPVTNNFHRIPGQLLHNSAEFHITQSLSDLGISDSEFTYFDNLPQEAETEFVPYQYDSDVLSGIEELDPLTDFTDIG

Target ORF information:

RefSeq Version	XM_026022821.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (LOC4330371), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026022821.1

ORF Insert Sequence:

ATGGCATCAA AAGGTCCTCG ATCAAAGTTA GATCATGAGA CAAGAGCCAG ACGCCAGAAG 
GCTCTTGAAG CCCCAAGGGG GGAGCCTCGA AGGACGAAAA CTCACTGGGA CCATCTTCTT 
GGAGAAATGG CTTGGCTGGC AAAGGAGTTT GATGCTGAGA GAAAATGGAA GCTGTCCATG 
GCTAAAAGGA TTGCTCAAAG AGCTAATAAG GGTGTAGTTG ACCAAGCAAC AAAGGACGAG 
AGAAAACAGA AGGAGGAAGA AGTTCGCCTG AGAAAAGTTG CCCTAAATAT TTCTAAGGAC 
GTGAAGAAGT TCTGGACCAA AATAGAGAAG CTGGTTTTTT ATAAGAACCA ACTGGAGCTT 
GAGGAAAGGA AAAAAAAGGC CCTAGACAAG CAACTTGATT TCCTCTTAGG ACAGACTGAA 
AGATATTCAA CAATGCTGGC AGAAAATCTT GTTGATGTGC GCCTTCAAAA TCAAGAAAAT 
GACTCTTTAC AGACTAATCA AAGATCCCAG CAGGAACTAG CACAAGAGAA CATAAATGCA 
TCAAGTCCGA CTGATGTTGA CAATGTGGAA ATCGATGATG ATTACAACAG TTCCTTGGGT 
GAAGAGCCAA AAGATGATGA ACACACAATC GATGAGGATG AAGCTCAAAT TACGGAGGCT 
GAGCGCAACG AGGAATTGGC TGCTTTGCAG GCAGAAGCTG ATCTACCACT GGATGATATT 
CTCAAGTTGT ACACTAAAAA TAAAGTTCAA GCTTTCTCAG TTAGCAGGGA AAGCAGTCCA 
GATGGCAGAG ACGTTTTTAG TGACTCAGAT TCAAAGGATT TGATTAAAGA TCCTTTGAAC 
CAGGCTAATG GATGCAACGA TGAATCTGAT CATACTTCAA GTGATGAAGG CATTTCTTCT 
GAGGAAGCAG ATGATTACCA GTCTTACTCT GAGTTTGTGA AAAAGAACAC TGTGAAATGC 
AATGGCAATA TCTCATCTGT TGATGCAAAG GACGACGAAG ATTATGTTGC TAATGATGAA 
GGAAAGGATG ATGAAGCTAC TTTGTCTGAA GAGGAAGAGT TGGCGAAGAA AGAAGACTGT 
GATCCTCTAG ATGAGGTTAA GTTACTGCAG AAAGAGAGTG AGATCCCACT TGAAGAACTT 
CTTGCAAGAT ACCAAAAGGA TGGCTATGCA GATGATGACA CAACAGAATT GGAAAATTCA 
CCTGCACTAT CAGTTGAGGA TGTCAATGCC AACATGCCTG TGGATGATGA GTCTGCAGAC 
ACCGTGGAGG TAAACAGGGA TCTTTCTGCA GATACCATGA AACTGACCAG AGATCAGTCT 
GCAGAGACCG TAAAAGTGAA TAATGACCAG TCTGCAGAGA TTGTGGAGGG CAACAATGAC 
ACATTTGAAG ATCATGAATC TGCAGGCATG CTGGGACCTG AGCATGTCAG TGGTAGTGTT 
CTACAACTAG AAACATCAGA GCCTATTGTA CAGGAAAATA CTGCTAAAGA GGGTGATGTA 
ACTGATACTA AAGCGATGGC CAATGGAGAT AATAGTGATG TGATTGCTGA TGCTGCAGCT 
GCTGCAAGAT CAGCACAACC GACTGGTAAC ACGTTCTCAA CAACAAATGT GCGGACAAAA 
TTCCCTTTCC TTCTTAAGCA TTCTCTACGA GAATATCAGC ACATTGGACT GGACTGGCTT 
GTTGCTATGT ATGAAAAGAG GCTGAATGGA ATTCTAGCAG ATGAAATGGG TTTGGGGAAG 
ACAATTATGA CCATCTCCTT GCTAGCACAC CTTGCATGTG AGAAGGGAAT ATGGGGTCCA 
CATCTTATTG TAGTGCCAAC TAGTGTTATG CTAAATTGGG AGACTGAATT TCTCAAATGG 
TGTCCTGCGT TTAAAATACT GACATATTTT GGAAGTGCAA AAGAGAGAAA ACAGAAACGT 
CAAGGTTGGA TGAAACCAAA TTACTTCCAT GTATGCATCA CTACATACAG GCTTGTTATT 
CAGGACTCCA AAGTGTTTAA GCGAAAAAAA TGGAAGTATC TTATTCTTGA TGAGGCTCAT 
TTGATAAAGA ACTGGAAATC ACAAAGATGG CAGACTTTAC TAAATTTTAA TTCGAAGCGA 
CGCATTCTTT TAACTGGAAC TCCTCTACAA AATGACCTCA TGGAACTTTG GTCTCTCATG 
CACTTTTTGA TGCCTCATGT ATTTCAGTCA CATCAAGAGT TCAAGGACTG GTTCTGCAAT 
CCAATTTCAG GCATGGTAGA GGGCCAAGAC AAGGTAAACA AAGAAGTGAT AGATCGGTTG 
CACAACGTCC TTCGTCCGTT TATACTTCGT CGATTGAAAC GGGATGTGGA GAAACAGTTG 
CCACAGAAGC ATGAGCATGT CATATATTGC CGACTTTCCA GAAGGCAAAG GAACTTGTAT 
GAAGATTTCA TTGCAAGCTC AGAGACACAG GCAACATTGG CAAGTGGAAA TTATTTTGGC 
ATGATTAGCA TCATTATGCA GCTTAGAAAG GTTTGTAACC ATCCAGATCT TTTTGAAGGC 
CGTCCAATTA TCAGCTCTTT TGACATGGCA GGGATCAACA TGCAAATCAG CTCTTCTGTT 
GGCATGGTCC TTGATAAAGG CCCATTTTCT CAGGTTGACC TGTCAGATAT GAATTTTGTA 
TTTACTCAAA ATGAATATAA TATGACTTCT TGGGAGGAAG ATGAGGTTGC TGCCATTTTT 
TCTCCAAGCA TAACCCTCAG GGGTTCTGGC ATTTCTCGCT CTACTAATGA TGGTCAGAGA 
AGCAATGGGA CAAATATTTT TGAAGAGATT CACAATTCTT TGTGGGAGGA GAGAATAAAA 
GAAGCTAATG AAAGAGCAGC TTCCATTGCA TGGTGGAATA GGGTAAGATG CCAGAAGAGG 
CCTGTTTATG GCACAAATAT TCGGGAGGTT TTGACCATAA AGCATCCTGT GTCTGATATT 
CTCGAGAAGA AGAAGAACCC TTTATGCCAC ATGGAATTTT CATCAAGCCT AGCAAATCTT 
GTTCTATCAT CGGTGGATCG CTTCAAGACA ATGCTTGATT TTATTGAGTC ATTTACTTTT 
GCAATTCCTG CTGCACGAGC GCCTGCACCA TTATTCTGGT GCAACAAAGG GAAATCTCCT 
GTTCTTATTG AACCAGCATA CAGAGAGAAA TGCATGAATG AGTTTTCTCC TGTTTTCTCT 
CCTATAAGGC CTGCGATTGT TCGCCGTCAA GTGTACTTTC CTGACAGGCG TTTGATCCAG 
TTTGACTGTG GAAAGTTGCA GGAACTTGCT ATTTTGTTGA GGCGTTTGAA GTCAGAAGGA 
CACAGGGCCT TGATATTTAC TCAGATGACT AAGATGCTTG ATATACTGGA GGAGTTCATT 
AATCTATATG GGTATACGTA TTTGCGGTTA GATGGATCAA CCCAGCCAGA AGAGAGGCAG 
ACACTGATGC AGAGGTTTAA TACTAACCCA AAGTTTTTTC TTTTTATCTT ATCCACTCGT 
AGTGGTGGTG TGGGAATCAA CCTAGTAGGT GCAGACACTG TCATTTTCTA TGATAGTGAC 
TGGAATCCTG CAATGGACCA ACAGGCCCAA GACAGATGCC ACAGGATAGG TCAAACTCGT 
GAAGTTCACA TCTATAGACT GATCAGTGAG AGCACTATAG AGGAGAATAT ACTCAAGAAA 
GCCAATCAGA AGCGAGCTCT TGATGATCTA GTGATACAAC GCGGTAGCTA CAATACAGAG 
TTCTTCAAGA AGCTTGATCC TATGGAATTC TTTTCTGGGC ACTCATCACT TCATGCAGAG 
AATCAGCAGA AGGATTGCTC TCTGTCTGCT GGACCCTCAA ACGGAACTAA TCTGGCGCTG 
TCAAATGTTG ATGTTGAAGC AGCTATTAGA CAGGCAGAAG ATGAAGCTGA CTATATGGCT 
CTCAAGAGGC TGGAGCAGGA AGAGGCCGTG GACAATCAAG AGTTCAGCGA GGAGGCTGCT 
GGAAGACTTG AGGAGGATGA TCTTGTAAAC GAGGATGACA CTAAACCTGA TGAACACACC 
AATGAAGAGC ATAAATATCA GTGTTCTGAT TTGGTCAAGG ACAAGCATGT TGCTTTATCT 
ATAAATCAAT TAGATGAAGA AAAGGCTATA ACTTTGGCTG GAGGAGATGG AGATATTGAC 
ATGCTTGCTG ATGTTAAGCA GATGGCTGCT GCAGCTGCTG CAGCAGGTCA AGCAAGTTCG 
TCTTTTGAAA ATCAGCTCCG GCCAATTGAT AGATATGCCA TGCGGTTTTT GGAACTCTGG 
GATCCAATAA TTGATAAAGC TGCTATAAAC TATCAGGTCA ATGTTGAAGA GGAAGAATGG 
GAACTTGAAC GCATTGAAAA ACTCAAAGAA GATTTAGAAG CAGAAATTGA TGAAGACCAG 
GAACCACTTT CTTATGAGTC ATGGGATGTT GATTTTGCTA CTACAGCATA TCGGCAACAT 
GTTGAGGCTC TAGCTCAAAA ACAGTTGTTT GAGGAACAAG AAAGACAAGC CCGTGAAGCA 
GCAAAAGAGT TGGAGGAAAA GAATGATAAT AGCACTCAAC GAAAAAAGTC AAAAAAGAAC 
AAGAAGAAGG CAGCGAAGTT CAAATCTTTG AAGAAAGGAC GTTTATCATC TGAGTCAGAG 
GTTATGGTTG AGGAAACATC AGTAGATACT ATGAGCATTG ATGACAATGC ACCCTCACCA 
GAGCTAATGA GCGATGAATC AGCACACCAT CATTCTAACA AGCATAAGAG GATAATGTCA 
ACCAATGAGG AAGAGAACAG CAATAGCAGA AGCCTGAAGA AGCTCAAGAA AGCCCCTAAA 
TCGAGTTTCA GTTCCGAGGC CTTATCACCT AAGCATTTTC TTGAAGGCAA GCAGCTTAAG 
TTGAAAGATG AACTTAATGA TTCTGATCCC AAATCGGGGG CAAGAATTAA GAGTGATGGC 
CGAATTTCTG TCCCATGCAT GCCAGTAAAG CGTGTCATGG TAATCAAGCC TGAGAGATTG 
AAGAAGAAGA GCCTATGGTC TCGCGATTGT GCTTCAGACT CGTGGACAAC TGAGGAAGAT 
GCTGTTCTTT GTGCAACTGT AAATGAGTAC GGCCCTCTTT GGGAACTGGC TAGTGATTCT 
CTTCATTCTG TACCAGGTGG TGCATTTTAT AGAGGAAGAT ACCGTCACCC TGTGCATTGC 
TGTGAGAGAT TCCGGGAATT AGTCTGCAAG CACATCTTAT CAGCAACAGA CAATTCAAAC 
AGTGAGAAGG TTCCTTCTGG GACTGGGAAG GCTATCTTGA AAGTATCTGA GGATCAAACT 
CAGATGTTGC TCAATGTAAT CAGCGAGCTT CCTAACAATG AATTGCTTCT CCAGAAACAT 
TTCATGGCTG TTCTTTCATC TGTTTGGAGA TCGAAGTCTG CCTGTGAGTC CCACTGTGTT 
ATGAGTTACT CTAACACACT CCAGAAACCT GGCAGACTCA GCGAGAACTG GTCCATGACA 
AACTTCAGAC CAAACTTTAA TCTAGTAAGG ACAGCCCTCG CAGATGCTCA GGTTCAATGT 
CCAAGAATGG TAGTTCCAAC AAGCAATCAT GAGTCCCGCC GTAACTTTTT GGAATTGGAA 
TTGGATTTCT TGACAGATCG AGATGATTAT GAGGCGGACT TTCCATCTGT TGTTAACGTG 
TCCATACTAG AACCGGAACC ATTAAAACAT GCTGTGGAGC CAGTGGAGCA CTCACTACTG 
TCCACACTTT CTTATAGACA TGCTGAGAAC AGATTCAGGA TGGTATCAGA AACTTGTTTT 
GAAGGGGAAG GTTCTCACTG GGCCTCATCT GCTTTCCACA CATACGATGC CGGTCGGCAT 
AAATCTGGTC CTAAGTCCAT AGGAAAGCAC AAAGCATCAT CAGAATCTGG AAGGCCTCCC 
AAATCCAAAA TTCAAAGGAC CACTGAACCA CAGGAAGTGC CGGTTACAAA TAATTTTCAT 
AGGATTCCGG GGCAACTTCT GCATAACTCA GCAGAGTTCC ACATTACCCA GTCCCTATCT 
GACCTTGGCA TCAGTGATTC TGAATTCACC TACTTTGATA ATCTTCCTCA AGAGGCTGAA 
ACAGAGTTTG TTCCGTACCA GTATGACTCT GATGTCCTTT CTGGCATTGA GGAGTTGGAT 
CCTCTTACAG ATTTCACAGA CATCGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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LOC4330371 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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