CCL2 cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	CCL2
Entrez Gene ID	100009130
Full Name	chemokine (C-C motif) ligand 2
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001082294.1	NP_001075763.1	C-C motif chemokine 2 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

[Peripheral blood endothelial progenitor cells enhance the expressions of SDF-1 and MCP-1 of bone marrow stromal cells and promote their homing ability].
Wei H, Li C, Zhao X, Yuan R, Dai X, Li Y, Liu L
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology32(1)20-4(2016 Jan)

[Dachengqi decoction reduces the serum levels of mast cell tryptase and inflammatory cytokines in rabbits with post-cardiac arrest syndrome].
Yang J, Xiao M, Wang X, Li X, Liang P, Chai L, Luo M
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology30(10)1026-9(2014 Oct)

Increased CCL2 expression and macrophage/monocyte migration during microbicide-induced vaginal irritation.
Alt C, Harrison T, Dousman L, Fujita N, Shew K, Tran TT, Shayesteh S, Matsukawa A, Mirsalis J, D'Andrea A
Current HIV research7(6)639-49(2009 Nov)

Effects of antiatherosclerosis in carotid artery by RNAi-mediated silencing of MCP-1 expression.
Hu ZJ, Liao Y, Hu LY, Wang JL, Li J, Lu WM, Wang SM
Annals of vascular surgery23(5)652-62(2009 Sep-Oct)

Neutrophil attractant/activation protein-1 and monocyte chemoattractant protein-1 in rabbit. cDNA cloning and their expression in spleen cells.
Yoshimura T, Yuhki N
Journal of immunology (Baltimore, Md. : 1950)146(10)3483-8(1991 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that peripheral blood endothelial progenitor cells can increase the expressions of chemokines CCL2 and CXCL12 and promote the migration of enhanced green fluorescent protein-positive bone marrow stromal cells to bone defect site.
Title: [Peripheral blood endothelial progenitor cells enhance the expressions of SDF-1 and MCP-1 of bone marrow stromal cells and promote their homing ability].

Data show that the serum levels of mast cell tryptase, monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) decreased significantly in Dachengqi decoction treatment group.
Title: [Dachengqi decoction reduces the serum levels of mast cell tryptase and inflammatory cytokines in rabbits with post-cardiac arrest syndrome].

measurements of CCL2 and macrophages can serve as new inflammatory biomarkers to evaluate the safety of promising novel microbicides for prevention of HIV.
Title: Increased CCL2 expression and macrophage/monocyte migration during microbicide-induced vaginal irritation.

Expression of MCP-1 was successfully inhibited by transfecting MCP-1 siRNA expression plasmid to the carotid artery, and the progression of atherosclerosis was restricted by RNAi-mediated silencing of MCP-1 expression.
Title: Effects of antiatherosclerosis in carotid artery by RNAi-mediated silencing of MCP-1 expression.

The following CCL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOb00298	NM_001082294.1 Latest version!	Oryctolagus cuniculus chemokine (C-C motif) ligand 2 (CCL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCL2 ( NM_001082294.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> NP_001075763.1 Oryctolagus cuniculus chemokine (C-C motif) ligand 2 (CCL2), mRNA.

Price & Availability↑

CloneID	OOb00298
Clone ID Related Accession (Same CDS sequence)	NM_001082294.1
Accession Version	NM_001082294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Oryctolagus cuniculus(rabbit)
Product	C-C motif chemokine 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M57440.1. ##Evidence-Data-START## Transcript exon combination :: M57440.1, CU465008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3321799, SAMEA3321800 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGTCT CTGCAACGCT TCTGTGCCTG CTGCTCATAG CAGTCGCCTT CAGCTCCCAT 
GTGCTTGCCC AGCCAGATGC CGTGAATTCC CCAGTCACCT GCTGCTATAC ATTCACCAAC 
AAGACCATCT CAGTGAAGAG GCTAATGAGC TATAGAAGAA TCAACAGCAC CAAGTGTCCC 
AAAGAAGCTG TGATCTTCAT GACCAAACTG GCCAAGGGGA TCTGTGCTGA CCCCAAGCAG 
AAGTGGGTCC AGGATGCCAT TGCCAACCTG GACAAGAAAA TGCAGACACC AAAGACTTTG 
ACCTCTTATT CCACAACCCA AGAACACACG ACTAATTTAT CTTCCACCCG GACACCCTCT 
ACTACTACTT CATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075763.1
CDS	16..393
Translation	MKVSATLLCLLLIAVAFSSHVLAQPDAVNSPVTCCYTFTNKTISVKRLMSYRRINSTKCPKEAVIFMTKLAKGICADPKQKWVQDAIANLDKKMQTPKTLTSYSTTQEHTTNLSSTRTPSTTTSL

Target ORF information:

RefSeq Version	NM_001082294.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus chemokine (C-C motif) ligand 2 (CCL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082294.1

ORF Insert Sequence:

ATGAAGGTCT CTGCAACGCT TCTGTGCCTG CTGCTCATAG CAGTCGCCTT CAGCTCCCAT 
GTGCTTGCCC AGCCAGATGC CGTGAATTCC CCAGTCACCT GCTGCTATAC ATTCACCAAC 
AAGACCATCT CAGTGAAGAG GCTAATGAGC TATAGAAGAA TCAACAGCAC CAAGTGTCCC 
AAAGAAGCTG TGATCTTCAT GACCAAACTG GCCAAGGGGA TCTGTGCTGA CCCCAAGCAG 
AAGTGGGTCC AGGATGCCAT TGCCAACCTG GACAAGAAAA TGCAGACACC AAAGACTTTG 
ACCTCTTATT CCACAACCCA AGAACACACG ACTAATTTAT CTTCCACCCG GACACCCTCT 
ACTACTACTT CATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[Peripheral blood endothelial progenitor cells enhance the expressions of SDF-1 and MCP-1 of bone marrow stromal cells and promote their homing ability].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology32(1)20-4(2016 Jan)
Wei H,Li C,Zhao X,Yuan R,Dai X,Li Y,Liu L

[Dachengqi decoction reduces the serum levels of mast cell tryptase and inflammatory cytokines in rabbits with post-cardiac arrest syndrome].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology30(10)1026-9(2014 Oct)
Yang J,Xiao M,Wang X,Li X,Liang P,Chai L,Luo M

Increased CCL2 expression and macrophage/monocyte migration during microbicide-induced vaginal irritation.
Current HIV research7(6)639-49(2009 Nov)
Alt C,Harrison T,Dousman L,Fujita N,Shew K,Tran TT,Shayesteh S,Matsukawa A,Mirsalis J,D'Andrea A

Effects of antiatherosclerosis in carotid artery by RNAi-mediated silencing of MCP-1 expression.
Annals of vascular surgery23(5)652-62(2009 Sep-Oct)
Hu ZJ,Liao Y,Hu LY,Wang JL,Li J,Lu WM,Wang SM

Neutrophil attractant/activation protein-1 and monocyte chemoattractant protein-1 in rabbit. cDNA cloning and their expression in spleen cells.
Journal of immunology (Baltimore, Md. : 1950)146(10)3483-8(1991 May)
Yoshimura T,Yuhki N