csf-3 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	csf-3
Entrez Gene ID	100499617
Full Name	granulocyte colony-stimulating factor precursor
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021556174.1	XP_021411849.1	granulocyte colony-stimulating factor precursor isoform X1
NM_001195184.1	NP_001182113.1	granulocyte colony-stimulating factor precursor

Pathways from BioSystems
Gene Ontology

The following csf-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the csf-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf01082	NM_001195184.1 Latest version!	Oncorhynchus mykiss granulocyte colony-stimulating factor precursor (csf-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OOf60449	XM_021556174.1 Latest version!	Oncorhynchus mykiss granulocyte colony-stimulating factor precursor (csf-3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

csf-3 ( NM_001195184.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001182113.1 Oncorhynchus mykiss granulocyte colony-stimulating factor precursor (csf-3), mRNA.

Price & Availability↑

CloneID	OOf01082
Clone ID Related Accession (Same CDS sequence)	NM_001195184.1
Accession Version	NM_001195184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Oncorhynchus mykiss(rainbow trout)
Product	granulocyte colony-stimulating factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AM982799.1. ##Evidence-Data-START## Transcript exon combination :: AM982799.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00041049, SAMN03784902 [ECO:0000348] ##Evidence-Data-END##

ATGAACACTC TCAAAATTGT AGGTCTGCTC TGCTGTTTAG CTTTTCTGGT CCGGTCCGCA 
CACCTTCCAG AGAGGTCAAT GGACGCAAGG AGTTTGGTCG AAAAAATATT AGGCAACATC 
CCCATGGTTC ACGAGTCTAG TGTCAAAATC CCGGGCCTGA CCCTTGACCC GTCCAGCCAG 
TCTAGGAACA TACCGTACCA GTCCATGGTG ACCTCCCTGG GCATCCCCGC AGCACCAGAA 
CTAAAGACTG TCTGCAGACC CCTAAACCAA ATCGACTTCA CCCTGGAGGT GTGTCTGAGC 
AGTATGTCTG CGGGGCTGCA GCTGTATCAG GATGTGCTGG GTGTGCTGAA GGAGCGTGTG 
ACCACTGAAA AGGTGACAGG ACTCCTGGCT GACATCAGAG ACCTGCTGGC CCAGGTCAAC 
AAGATGCAGG AGCCGGGCCA GATGAGCAGT GTGGCCCAGT ACGAGGCCTC GGGATTGGCC 
TCACGCCTCC CAGGGGACTA CGAGGTTCAG GTTGCCACTC ATTTTATCCT GTTGCAGCTC 
CGTGACTTCA CACAGAACCT AAAACGCAGC CTGCGCAACG TCGAACACCT GACTTCCAGG 
CCAGGACAGA GCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182113.1
CDS	56..673
Translation	MNTLKIVGLLCCLAFLVRSAHLPERSMDARSLVEKILGNIPMVHESSVKIPGLTLDPSSQSRNIPYQSMVTSLGIPAAPELKTVCRPLNQIDFTLEVCLSSMSAGLQLYQDVLGVLKERVTTEKVTGLLADIRDLLAQVNKMQEPGQMSSVAQYEASGLASRLPGDYEVQVATHFILLQLRDFTQNLKRSLRNVEHLTSRPGQSG

Target ORF information:

RefSeq Version	NM_001195184.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss granulocyte colony-stimulating factor precursor (csf-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195184.1

ORF Insert Sequence:

ATGAACACTC TCAAAATTGT AGGTCTGCTC TGCTGTTTAG CTTTTCTGGT CCGGTCCGCA 
CACCTTCCAG AGAGGTCAAT GGACGCAAGG AGTTTGGTCG AAAAAATATT AGGCAACATC 
CCCATGGTTC ACGAGTCTAG TGTCAAAATC CCGGGCCTGA CCCTTGACCC GTCCAGCCAG 
TCTAGGAACA TACCGTACCA GTCCATGGTG ACCTCCCTGG GCATCCCCGC AGCACCAGAA 
CTAAAGACTG TCTGCAGACC CCTAAACCAA ATCGACTTCA CCCTGGAGGT GTGTCTGAGC 
AGTATGTCTG CGGGGCTGCA GCTGTATCAG GATGTGCTGG GTGTGCTGAA GGAGCGTGTG 
ACCACTGAAA AGGTGACAGG ACTCCTGGCT GACATCAGAG ACCTGCTGGC CCAGGTCAAC 
AAGATGCAGG AGCCGGGCCA GATGAGCAGT GTGGCCCAGT ACGAGGCCTC GGGATTGGCC 
TCACGCCTCC CAGGGGACTA CGAGGTTCAG GTTGCCACTC ATTTTATCCT GTTGCAGCTC 
CGTGACTTCA CACAGAACCT AAAACGCAGC CTGCGCAACG TCGAACACCT GACTTCCAGG 
CCAGGACAGA GCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

csf-3 ( XM_021556174.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021411849.1 Oncorhynchus mykiss granulocyte colony-stimulating factor precursor (csf-3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf60449
Clone ID Related Accession (Same CDS sequence)	XM_021556174.1
Accession Version	XM_021556174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	granulocyte colony-stimulating factor precursor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035089.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCAA GGAGTTTGGT CGAAAAAATA TTAGGCAACA TCCCCATGGT TCACGGGTCT 
AGTGTCAAAA TCCCGGGCCT GACCCTTGAC CCGTCCAGCC AGTCTAGGAA CATACCGTAC 
CAGTCCATGG TGACCTCCCT GGGCATCCCC GCAGCACCAG AACTAAAGAC TGTCTGCAGA 
CCCCTAAACC AAATCGACTT CACCCTGGAG GTGTGTCTGA GCAGTATGTC TGCGGGGCTG 
CAGCTGTATC AGGATGTGCT GGGTGTGCTG AAGGAGCGTG TGACCACTGA CAAGGTGACA 
GGACTCCTGG CTGACATCAG AGACCTGCTG GCCCAGGTCA ACAAGATGCA GGAGCCGGGC 
CAGATGAGCA GTGTGGCCCA GTACGAGGCC TCGGGACTGG CCTCACGCCT CCCAGGGGAC 
TACGAGGTTC AGGTTGCCAC TCATTTTATC CTGTTGCAGC TCCGTGACTT CACACAGAAC 
CTAAAACGCA GCCTGCGCAA CGTCGAACAC CTGACTTCCA GGCCAGGACA GAGCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021411849.1
CDS	208..747
Translation	MDARSLVEKILGNIPMVHGSSVKIPGLTLDPSSQSRNIPYQSMVTSLGIPAAPELKTVCRPLNQIDFTLEVCLSSMSAGLQLYQDVLGVLKERVTTDKVTGLLADIRDLLAQVNKMQEPGQMSSVAQYEASGLASRLPGDYEVQVATHFILLQLRDFTQNLKRSLRNVEHLTSRPGQSG

Target ORF information:

RefSeq Version	XM_021556174.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss granulocyte colony-stimulating factor precursor (csf-3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021556174.1

ORF Insert Sequence:

ATGGACGCAA GGAGTTTGGT CGAAAAAATA TTAGGCAACA TCCCCATGGT TCACGGGTCT 
AGTGTCAAAA TCCCGGGCCT GACCCTTGAC CCGTCCAGCC AGTCTAGGAA CATACCGTAC 
CAGTCCATGG TGACCTCCCT GGGCATCCCC GCAGCACCAG AACTAAAGAC TGTCTGCAGA 
CCCCTAAACC AAATCGACTT CACCCTGGAG GTGTGTCTGA GCAGTATGTC TGCGGGGCTG 
CAGCTGTATC AGGATGTGCT GGGTGTGCTG AAGGAGCGTG TGACCACTGA CAAGGTGACA 
GGACTCCTGG CTGACATCAG AGACCTGCTG GCCCAGGTCA ACAAGATGCA GGAGCCGGGC 
CAGATGAGCA GTGTGGCCCA GTACGAGGCC TCGGGACTGG CCTCACGCCT CCCAGGGGAC 
TACGAGGTTC AGGTTGCCAC TCATTTTATC CTGTTGCAGC TCCGTGACTT CACACAGAAC 
CTAAAACGCA GCCTGCGCAA CGTCGAACAC CTGACTTCCA GGCCAGGACA GAGCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.