defb3 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	defb3
Entrez Gene ID	100499616
Full Name	defensin beta 3
Synonyms	db-3
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001195183.2	NP_001182112.2	beta-defensin 3 precursor
NM_001195183.1	NP_001182112.1	beta-defensin 3 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Characterization of the rainbow trout transcriptome using Sanger and 454-pyrosequencing approaches.
Salem M, Rexroad CE 3rd, Wang J, Thorgaard GH, Yao J
BMC genomics11564(2010 Oct)

Characterization of three novel beta-defensin antimicrobial peptides in rainbow trout (Oncorhynchus mykiss).
Casadei E, Wang T, Zou J, Gonz?lez Vecino JL, Wadsworth S, Secombes CJ
Molecular immunology46(16)3358-66(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following defb3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the defb3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf01078	NM_001195183.1 Latest version!	Oncorhynchus mykiss defensin beta 3 (defb3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OOf60713	NM_001195183.2 Latest version!	Oncorhynchus mykiss defensin beta 3 (defb3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

defb3 ( NM_001195183.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001182112.1 Oncorhynchus mykiss defensin beta 3 (defb3), mRNA.

Price & Availability↑

CloneID	OOf01078
Clone ID Related Accession (Same CDS sequence)	NM_001195183.1
Accession Version	NM_001195183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Oncorhynchus mykiss(rainbow trout)
Product	beta-defensin 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FM212657.1. ##Evidence-Data-START## Transcript exon combination :: FM212657.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00041048, SAMD00041049 [ECO:0000348] ##Evidence-Data-END##

ATGCGCCTGA TGATCTTGAT GCTAGTGGCT CTTTTAGTCA TTGCTTGTGG AATACAAGAG 
TCATCTGCAT CTCTACACTT ATGTTTCATT AGTGGGGGCG GGTGCAGAAA CCTTCGTTTG 
TGCCTTGCTT CTGGTGGTAC TAACATTGGA AAAATGGGAT GTACATGGCC GAATGTATGC 
TGTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182112.1
CDS	22..210
Translation	MRLMILMLVALLVIACGIQESSASLHLCFISGGGCRNLRLCLASGGTNIGKMGCTWPNVCCK

Target ORF information:

RefSeq Version	NM_001195183.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss defensin beta 3 (defb3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195183.1

ORF Insert Sequence:

ATGCGCCTGA TGATCTTGAT GCTAGTGGCT CTTTTAGTCA TTGCTTGTGG AATACAAGAG 
TCATCTGCAT CTCTACACTT ATGTTTCATT AGTGGGGGCG GGTGCAGAAA CCTTCGTTTG 
TGCCTTGCTT CTGGTGGTAC TAACATTGGA AAAATGGGAT GTACATGGCC GAATGTATGC 
TGTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

defb3 ( NM_001195183.2 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001182112.2 Oncorhynchus mykiss defensin beta 3 (defb3), mRNA.

Price & Availability↑

CloneID	OOf60713
Clone ID Related Accession (Same CDS sequence)	NM_001195183.2
Accession Version	NM_001195183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-09
Organism	Oncorhynchus mykiss(rainbow trout)
Product	beta-defensin 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EZ763638.1. On Jun 5, 2018 this sequence version replaced NM_001195183.1. ##Evidence-Data-START## Transcript exon combination :: EZ763638.1, GFIN01021093.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00041048, SAMD00041049 [ECO:0000348] ##Evidence-Data-END##

ATGAACTGCC TGATGATCTT CATGCTAGTG GCTCTTTTAG TCATTGCTTG TGGAATACAA 
GAGTCATCTG CATCTCTACA CTTATGTTTC ATTAGTGGGG GCGGGTGCAG AAACCTTCGT 
TTGTGCCTTG CTTCTGGTGG TACTAACATT GGAAAAATGG GATGTACATG GCCGAATGTA 
TGCTGTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182112.2
CDS	91..282
Translation	MNCLMIFMLVALLVIACGIQESSASLHLCFISGGGCRNLRLCLASGGTNIGKMGCTWPNVCCK

Target ORF information:

RefSeq Version	NM_001195183.2
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss defensin beta 3 (defb3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195183.2

ORF Insert Sequence:

ATGAACTGCC TGATGATCTT CATGCTAGTG GCTCTTTTAG TCATTGCTTG TGGAATACAA 
GAGTCATCTG CATCTCTACA CTTATGTTTC ATTAGTGGGG GCGGGTGCAG AAACCTTCGT 
TTGTGCCTTG CTTCTGGTGG TACTAACATT GGAAAAATGG GATGTACATG GCCGAATGTA 
TGCTGTAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Characterization of the rainbow trout transcriptome using Sanger and 454-pyrosequencing approaches.
BMC genomics11564(2010 Oct)
Salem M,Rexroad CE 3rd,Wang J,Thorgaard GH,Yao J

Characterization of three novel beta-defensin antimicrobial peptides in rainbow trout (Oncorhynchus mykiss).
Molecular immunology46(16)3358-66(2009 Oct)
Casadei E,Wang T,Zou J,Gonz?lez Vecino JL,Wadsworth S,Secombes CJ