LOC104105003 cDNA ORF clone, Nicotiana tomentosiformis

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104105003
Entrez Gene ID	104105003
Full Name	5-oxoprolinase
Gene Type	protein-coding
Organism	Nicotiana tomentosiformis

mRNA and Protein(s)

mRNA	Protein	Name
XM_009613233.2	XP_009611528.1	5-oxoprolinase

Pathways from BioSystems
Gene Ontology

The following LOC104105003 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104105003 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONi103841	XM_009613233.2 Latest version!	Nicotiana tomentosiformis 5-oxoprolinase (LOC104105003), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104105003 ( XM_009613233.2 ) cDNA ORF clone, Nicotiana tomentosiformis -> XP_009611528.1 Nicotiana tomentosiformis 5-oxoprolinase (LOC104105003), mRNA.

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CloneID	ONi103841
Clone ID Related Accession (Same CDS sequence)	XM_009613233.2
Accession Version	XM_009613233.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Nicotiana tomentosiformis
Product	5-oxoprolinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008896110.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 19, 2016 this sequence version replaced XM_009613233.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nicotiana tomentosiformis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC AAAGTGAAGG AAAACTTAAA TTTTGTATCG ACAGAGGGGG CACTTTTACT 
GATGTCTATG CAGAAATTCC TGGAAAATCT GAAGGCCGAG TCATGAAGTT GTTGTCAGTT 
GATCCTTCTA ATTATGATGA TGCTCCAGTT GAAGGAATAA GGAGGATTTT GGAGGAATTT 
ACTGGAAAGA AAATTCCCCG TAGTTCGAAA ATTCCTACTG ATAAGATCGA ATGGGTGAGG 
ATGGGTACAA CCGTGGCAAC AAATGCATTG TTGGAGAGGA AAGGGGAGAG AATTGCACTC 
TGTGTCACTC GAGGCTTTAG GGATTTGCTC CAGATTGGTA ATCAGGCTCG TCCGCATATA 
TTTGACCTCA CTGTATCAAA ACCATCAAAT CTTTATGAGG AGGTTGTAGA GGTTGATGAG 
AGAGTTGAGC TTGTTCTTGA TAAGGAGGGA GGAGTAAATT TAGACTCTTC TGCATCACTT 
GTTCAAGGTG TTTCAGGCGA GCTTGTCAAG GTTGTAAAGC CACTCGATGA GGAAGCTCTG 
AAACCTTTGC TGAATGCTTT GCTGCAAAGG GGTATTAGTT GCTTAGCTGT GGTTTTGCTC 
CACTCTTATA CTTTTCCCCA GCATGAAATA CTTCTGGAAA AGTTAGCTCT TAGTTTGGGC 
TTCAGGCATG TATCTATATC ATCTGCTTTG ACTCCTATGG TCCGAGCTGT TCCTCGAGGA 
TTTACAGCAA GTGTAGATGC ATATTTGACT CCTGTTATTA AAGAATACTT GTCAGGTTTC 
ATGTCCAAAT TTGATGAAGG CCTAGGAAAG TTGAATGTTT TATTTATGCA GTCGGATGGA 
GGGCTTGCAC CAGAACATAG ATTTTCAGGC CACAAAGCAA TTTTGTCAGG TCCTGCTGGT 
GGTGTAGTTG GTTACTCTCA GACACTCTTT GGGATTGAAA CAGACAAGGC CCTAATCGGT 
TTTGACATGG GTGGTACCTC TACTGATGTC AGCCGATATG CTGGTACCTA TGAACAAGTT 
ATAGAAACCC AGGTTGCTGG TGCAATAATT CAAGCACCTC AGCTCGATGT AAACACAGTC 
GCTGCTGGCG GTGGATCAAA GTTAAAATTT CAGTTTGGTT CTTTCCGGGT GGGACCAGAT 
TCAGTGGGTG CACACCCAGG ACCAGTTTGT TACCGGAAAG GCGGGCAACT CTCAGTTACA 
GATGCAAATC TTATCTTAGG ATATGTCATT CCTGATTTTT TCCCGTCCAT TTTTGGTCCC 
AATGAGGACC AGCCATTAGA CATTGATGCT ACGAGGGAAG AGTTTGAAAA ACTTGCGAGG 
CAAATCAATT CCTACAGAAA GAGCCAGGAT TCATCTGCAA GGGACATGAC GGTAGAGGAG 
ATTGCACAAG GTTTTGTGAA TGTTGCCAAT GAGACCATGT GCCGCCCAAT ACGGCAATTG 
ACAGAAATGA AGGGTCATGA AACAAGCCAC CATGCTCTTG CTTGCTTCGG AGGTGCAGGT 
CCTCAACATG CCTGTGCCAT TGCTAGATCA CTTGGAATGA AGGAAGTTCT TATTCACAGG 
TTATGTGGAA TCTTAAGTGC ATATGGTATG GGGTTAGCTG ATGTGGTAGA AGATGCGCAA 
GAGCCGTATT CTGCTGTTTA TGGCCCTGAT TCTGTGATTG AAGCTTCTCG CAGAGAAACT 
ATCTTGCTGA AGCAGGTGAT GGATAAGCTG CAGGAGCAAG GATTCAGAGA AGAAAGTATC 
ACCAGCGAAA CATACTTAAA CTTAAGGTAT GAGGGAACAG ACACCGCAAT CATGGTAAAG 
AGACCAACCA ATGATGATGG GTCACCTCCT GATTATGCTG CCGAATTTGT CAAGTTATTC 
CAGCGGGAGT ACGGATTTAA ACTTCAAAAC AGGAATATAG TTATATGTGA TGTTAGGGTT 
CGCGGTGTTG GAGTCACTAA CATACTGAAG CCTCGAGCCC TAGATTCTTC CCCTGGTGCC 
CCTAAAATTG AAAGTCACTA CAAAGTATAC TTTGGGAATG GATGGAATGA CACTCCTTTA 
TTTAAGCTTG AAAATCTGTC ATATGGGAAT GTTGTTCCTG GCCCAGCAAT TATCATGAAT 
GGCAATAGCA CTGTCATTGT TGAGCCCAAC TGCAAGGCCA TTATTACAAA ATATGGCAAC 
ATTAAAATAG AGATCGAGTC GGCTTCCAAT ACTGCAAAAG TAGATGAAAA AGTTGCAGAT 
GTTGTGCAGC TCTCAATTTT CAACAACCGG TTTATGGGAA TAGCTGAACA GATGGGGCGC 
ACCTTGCAGA GGACTTCTAT ATCAACAAAT ATTAAAGAGC GGCTAGACTT TTCTTGCGCA 
CTTTTTGGCC CTGATGGTGG GCTTGTGGCA AATGCACCCC ATGTTCCTGT GCACTTGGGG 
GCTATGTCGA GCACTGTTCG GTGGCAGCTG AAGCACTGGG GTGATAATCT AAATGAGGGA 
GATGTTTTAG TTGCCAATCA TCCTTCTGCT GGTGGTAGCC ACCTGCCTGA TATAACTGTA 
ATTACACCTG TTTTTAACAA AGGTAGATTG ATATTCTTTG TTGCAAGTAG AGGGCATCAT 
GCAGAGATTG GAGGTATTAC TCCTGGAAGT ATGCCGCCTT TCTCAAAGTT CATATGGGAG 
GAAGGAGCAG CAATAAAAGC ATTCAAGCTT GTTGAGAAGG GAACTTTTCA AGAAGAAGGA 
ATCACCAAAC TTCTTCGTTA CCCTTGTTCT GAGGAATCAA CTCATAACAT TCCAGGAACG 
CGCAGGCTTC AAGATAATTT GTCTGACCTT CATGCACAAG TAGCTGCAAA TCAGAGAGGA 
ATTACCCTTA TCAACGAGCT TATTGAGCAA TATGGTCTGG AAACTGTCCA AGCATACATG 
AACTATGTTC AGGCTAATGC TGAAGAAGCA GTGAGAGAAA TGCTTAAATC TGTTGCCGCG 
AGAGTTTCTT CTGAAGCAAA AATGTCCGGA AAGGAAGGCG TAGTGACTAT TGAAGAGGAA 
GATTACATGG ATGATGGGTC TACCATACAC TTAAAGCTTA CTATAGATTC TATGAAGGGA 
GAAGCATTTT TTGATTTCTC CGGAACCAGC CCAGAAGTTT ATGGTAATTG GAATGCCCCA 
GAGGCAGTGA CAGCTGCTGC AGTCATTTAC TGCGTACGCT GTTTGGTGAA CGTTGATATC 
CCTCTCAATC AAGGTTGCTT GGCTCCTGTA AAGATTTATA TACCTCCTGG TTCATTTCTC 
TCTCCAAGTG ACAAGGCTGC AGTGGTGGGT GGTAATGTCC TTACGTCACA GAGGATAACT 
GACGTCGTTC TTACTGCATT CCAAGCTTGT GCCTGCTCAC AGGGTTGCAT GAATAATTTA 
ACCTTTGGGG ATGACACTTT TGGTTACTAT GAAACAATTG GAGGTGGAAG TGGAGCTGGT 
CCTACTTGGG ATGGGACGAG TGCAGTCCAG TGTCACATGA CCAACACCCG AATGACAGAT 
CCAGAGATCT TTGAGCAAAG ATACCCAGTT ATATTGCATA AGTTTGGAAT AAGAGAGAAT 
AGTGGAGGTG CTGGACAACA TAAAGGAGGT GATGGCATTA TCAGGGAGAT TGAATTCAGG 
CGGCCGGTCA TTGTGAGCAT CCTATCAGAA AGGCGTGTGC ATGCACCGAG GGGGCTGATG 
GGTGGAAAAG ATGGGGCTCG AGGTGCTAAT TTCCTCGTCA CGAAAGATAA AAGGAAGGTG 
TATGTTGGTG GTAAAAACAC AATCGAGGTG CAGGCTGGGG AAATACTTCA GATTTTGACA 
CCAGGAGGAG GTGGCTGGGG TTCAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009611528.1
CDS	326..4135
Translation	MGSQSEGKLKFCIDRGGTFTDVYAEIPGKSEGRVMKLLSVDPSNYDDAPVEGIRRILEEFTGKKIPRSSKIPTDKIEWVRMGTTVATNALLERKGERIALCVTRGFRDLLQIGNQARPHIFDLTVSKPSNLYEEVVEVDERVELVLDKEGGVNLDSSASLVQGVSGELVKVVKPLDEEALKPLLNALLQRGISCLAVVLLHSYTFPQHEILLEKLALSLGFRHVSISSALTPMVRAVPRGFTASVDAYLTPVIKEYLSGFMSKFDEGLGKLNVLFMQSDGGLAPEHRFSGHKAILSGPAGGVVGYSQTLFGIETDKALIGFDMGGTSTDVSRYAGTYEQVIETQVAGAIIQAPQLDVNTVAAGGGSKLKFQFGSFRVGPDSVGAHPGPVCYRKGGQLSVTDANLILGYVIPDFFPSIFGPNEDQPLDIDATREEFEKLARQINSYRKSQDSSARDMTVEEIAQGFVNVANETMCRPIRQLTEMKGHETSHHALACFGGAGPQHACAIARSLGMKEVLIHRLCGILSAYGMGLADVVEDAQEPYSAVYGPDSVIEASRRETILLKQVMDKLQEQGFREESITSETYLNLRYEGTDTAIMVKRPTNDDGSPPDYAAEFVKLFQREYGFKLQNRNIVICDVRVRGVGVTNILKPRALDSSPGAPKIESHYKVYFGNGWNDTPLFKLENLSYGNVVPGPAIIMNGNSTVIVEPNCKAIITKYGNIKIEIESASNTAKVDEKVADVVQLSIFNNRFMGIAEQMGRTLQRTSISTNIKERLDFSCALFGPDGGLVANAPHVPVHLGAMSSTVRWQLKHWGDNLNEGDVLVANHPSAGGSHLPDITVITPVFNKGRLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGTFQEEGITKLLRYPCSEESTHNIPGTRRLQDNLSDLHAQVAANQRGITLINELIEQYGLETVQAYMNYVQANAEEAVREMLKSVAARVSSEAKMSGKEGVVTIEEEDYMDDGSTIHLKLTIDSMKGEAFFDFSGTSPEVYGNWNAPEAVTAAAVIYCVRCLVNVDIPLNQGCLAPVKIYIPPGSFLSPSDKAAVVGGNVLTSQRITDVVLTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPTWDGTSAVQCHMTNTRMTDPEIFEQRYPVILHKFGIRENSGGAGQHKGGDGIIREIEFRRPVIVSILSERRVHAPRGLMGGKDGARGANFLVTKDKRKVYVGGKNTIEVQAGEILQILTPGGGGWGSK

Target ORF information:

RefSeq Version	XM_009613233.2
Organism	Nicotiana tomentosiformis
Definition	Nicotiana tomentosiformis 5-oxoprolinase (LOC104105003), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009613233.2

ORF Insert Sequence:

ATGGGGAGTC AAAGTGAAGG AAAACTTAAA TTTTGTATCG ACAGAGGGGG CACTTTTACT 
GATGTCTATG CAGAAATTCC TGGAAAATCT GAAGGCCGAG TCATGAAGTT GTTGTCAGTT 
GATCCTTCTA ATTATGATGA TGCTCCAGTT GAAGGAATAA GGAGGATTTT GGAGGAATTT 
ACTGGAAAGA AAATTCCCCG TAGTTCGAAA ATTCCTACTG ATAAGATCGA ATGGGTGAGG 
ATGGGTACAA CCGTGGCAAC AAATGCATTG TTGGAGAGGA AAGGGGAGAG AATTGCACTC 
TGTGTCACTC GAGGCTTTAG GGATTTGCTC CAGATTGGTA ATCAGGCTCG TCCGCATATA 
TTTGACCTCA CTGTATCAAA ACCATCAAAT CTTTATGAGG AGGTTGTAGA GGTTGATGAG 
AGAGTTGAGC TTGTTCTTGA TAAGGAGGGA GGAGTAAATT TAGACTCTTC TGCATCACTT 
GTTCAAGGTG TTTCAGGCGA GCTTGTCAAG GTTGTAAAGC CACTCGATGA GGAAGCTCTG 
AAACCTTTGC TGAATGCTTT GCTGCAAAGG GGTATTAGTT GCTTAGCTGT GGTTTTGCTC 
CACTCTTATA CTTTTCCCCA GCATGAAATA CTTCTGGAAA AGTTAGCTCT TAGTTTGGGC 
TTCAGGCATG TATCTATATC ATCTGCTTTG ACTCCTATGG TCCGAGCTGT TCCTCGAGGA 
TTTACAGCAA GTGTAGATGC ATATTTGACT CCTGTTATTA AAGAATACTT GTCAGGTTTC 
ATGTCCAAAT TTGATGAAGG CCTAGGAAAG TTGAATGTTT TATTTATGCA GTCGGATGGA 
GGGCTTGCAC CAGAACATAG ATTTTCAGGC CACAAAGCAA TTTTGTCAGG TCCTGCTGGT 
GGTGTAGTTG GTTACTCTCA GACACTCTTT GGGATTGAAA CAGACAAGGC CCTAATCGGT 
TTTGACATGG GTGGTACCTC TACTGATGTC AGCCGATATG CTGGTACCTA TGAACAAGTT 
ATAGAAACCC AGGTTGCTGG TGCAATAATT CAAGCACCTC AGCTCGATGT AAACACAGTC 
GCTGCTGGCG GTGGATCAAA GTTAAAATTT CAGTTTGGTT CTTTCCGGGT GGGACCAGAT 
TCAGTGGGTG CACACCCAGG ACCAGTTTGT TACCGGAAAG GCGGGCAACT CTCAGTTACA 
GATGCAAATC TTATCTTAGG ATATGTCATT CCTGATTTTT TCCCGTCCAT TTTTGGTCCC 
AATGAGGACC AGCCATTAGA CATTGATGCT ACGAGGGAAG AGTTTGAAAA ACTTGCGAGG 
CAAATCAATT CCTACAGAAA GAGCCAGGAT TCATCTGCAA GGGACATGAC GGTAGAGGAG 
ATTGCACAAG GTTTTGTGAA TGTTGCCAAT GAGACCATGT GCCGCCCAAT ACGGCAATTG 
ACAGAAATGA AGGGTCATGA AACAAGCCAC CATGCTCTTG CTTGCTTCGG AGGTGCAGGT 
CCTCAACATG CCTGTGCCAT TGCTAGATCA CTTGGAATGA AGGAAGTTCT TATTCACAGG 
TTATGTGGAA TCTTAAGTGC ATATGGTATG GGGTTAGCTG ATGTGGTAGA AGATGCGCAA 
GAGCCGTATT CTGCTGTTTA TGGCCCTGAT TCTGTGATTG AAGCTTCTCG CAGAGAAACT 
ATCTTGCTGA AGCAGGTGAT GGATAAGCTG CAGGAGCAAG GATTCAGAGA AGAAAGTATC 
ACCAGCGAAA CATACTTAAA CTTAAGGTAT GAGGGAACAG ACACCGCAAT CATGGTAAAG 
AGACCAACCA ATGATGATGG GTCACCTCCT GATTATGCTG CCGAATTTGT CAAGTTATTC 
CAGCGGGAGT ACGGATTTAA ACTTCAAAAC AGGAATATAG TTATATGTGA TGTTAGGGTT 
CGCGGTGTTG GAGTCACTAA CATACTGAAG CCTCGAGCCC TAGATTCTTC CCCTGGTGCC 
CCTAAAATTG AAAGTCACTA CAAAGTATAC TTTGGGAATG GATGGAATGA CACTCCTTTA 
TTTAAGCTTG AAAATCTGTC ATATGGGAAT GTTGTTCCTG GCCCAGCAAT TATCATGAAT 
GGCAATAGCA CTGTCATTGT TGAGCCCAAC TGCAAGGCCA TTATTACAAA ATATGGCAAC 
ATTAAAATAG AGATCGAGTC GGCTTCCAAT ACTGCAAAAG TAGATGAAAA AGTTGCAGAT 
GTTGTGCAGC TCTCAATTTT CAACAACCGG TTTATGGGAA TAGCTGAACA GATGGGGCGC 
ACCTTGCAGA GGACTTCTAT ATCAACAAAT ATTAAAGAGC GGCTAGACTT TTCTTGCGCA 
CTTTTTGGCC CTGATGGTGG GCTTGTGGCA AATGCACCCC ATGTTCCTGT GCACTTGGGG 
GCTATGTCGA GCACTGTTCG GTGGCAGCTG AAGCACTGGG GTGATAATCT AAATGAGGGA 
GATGTTTTAG TTGCCAATCA TCCTTCTGCT GGTGGTAGCC ACCTGCCTGA TATAACTGTA 
ATTACACCTG TTTTTAACAA AGGTAGATTG ATATTCTTTG TTGCAAGTAG AGGGCATCAT 
GCAGAGATTG GAGGTATTAC TCCTGGAAGT ATGCCGCCTT TCTCAAAGTT CATATGGGAG 
GAAGGAGCAG CAATAAAAGC ATTCAAGCTT GTTGAGAAGG GAACTTTTCA AGAAGAAGGA 
ATCACCAAAC TTCTTCGTTA CCCTTGTTCT GAGGAATCAA CTCATAACAT TCCAGGAACG 
CGCAGGCTTC AAGATAATTT GTCTGACCTT CATGCACAAG TAGCTGCAAA TCAGAGAGGA 
ATTACCCTTA TCAACGAGCT TATTGAGCAA TATGGTCTGG AAACTGTCCA AGCATACATG 
AACTATGTTC AGGCTAATGC TGAAGAAGCA GTGAGAGAAA TGCTTAAATC TGTTGCCGCG 
AGAGTTTCTT CTGAAGCAAA AATGTCCGGA AAGGAAGGCG TAGTGACTAT TGAAGAGGAA 
GATTACATGG ATGATGGGTC TACCATACAC TTAAAGCTTA CTATAGATTC TATGAAGGGA 
GAAGCATTTT TTGATTTCTC CGGAACCAGC CCAGAAGTTT ATGGTAATTG GAATGCCCCA 
GAGGCAGTGA CAGCTGCTGC AGTCATTTAC TGCGTACGCT GTTTGGTGAA CGTTGATATC 
CCTCTCAATC AAGGTTGCTT GGCTCCTGTA AAGATTTATA TACCTCCTGG TTCATTTCTC 
TCTCCAAGTG ACAAGGCTGC AGTGGTGGGT GGTAATGTCC TTACGTCACA GAGGATAACT 
GACGTCGTTC TTACTGCATT CCAAGCTTGT GCCTGCTCAC AGGGTTGCAT GAATAATTTA 
ACCTTTGGGG ATGACACTTT TGGTTACTAT GAAACAATTG GAGGTGGAAG TGGAGCTGGT 
CCTACTTGGG ATGGGACGAG TGCAGTCCAG TGTCACATGA CCAACACCCG AATGACAGAT 
CCAGAGATCT TTGAGCAAAG ATACCCAGTT ATATTGCATA AGTTTGGAAT AAGAGAGAAT 
AGTGGAGGTG CTGGACAACA TAAAGGAGGT GATGGCATTA TCAGGGAGAT TGAATTCAGG 
CGGCCGGTCA TTGTGAGCAT CCTATCAGAA AGGCGTGTGC ATGCACCGAG GGGGCTGATG 
GGTGGAAAAG ATGGGGCTCG AGGTGCTAAT TTCCTCGTCA CGAAAGATAA AAGGAAGGTG 
TATGTTGGTG GTAAAAACAC AATCGAGGTG CAGGCTGGGG AAATACTTCA GATTTTGACA 
CCAGGAGGAG GTGGCTGGGG TTCAAAATGA