NCU02036 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU02036
Entrez Gene ID	3880798
Full Name	tRNA-splicing endonuclease
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_959565.3	XP_964658.3	tRNA-splicing endonuclease

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_02784	XP_003720957.1
Neurospora crassa	NCU02036	XP_964658.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU02036 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU02036 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12904	XM_959565.3 Latest version!	Neurospora crassa OR74A tRNA-splicing endonuclease (NCU02036), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU02036 ( XM_959565.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_964658.3 Neurospora crassa OR74A tRNA-splicing endonuclease (NCU02036), mRNA.

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CloneID	ONf12904
Clone ID Related Accession (Same CDS sequence)	XM_959565.3
Accession Version	XM_959565.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	tRNA-splicing endonuclease
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_959565.2.

ATGTCAGACA AAATGGAATA CCCAGAGCTG GGGACCGCGG ACGTTCAGAT GGCTATGATG 
GAGATGCATC AAGCCTTTGA AGAGTTTCCT CCAGACGCAC ATCCAATTTG CCCTTTGCTC 
AGCGACGATG ACGATACGGA CTACGCGTTG CCAGACCTCC CCGATGCGAA CGGAACAACG 
GTGGCAGAAA AGCAAAAGCG AATCGCTGAC GGGTTCGAGA GACGTAGAAT TACCTACGGC 
CTTTCGCTTC TGTTGGTGAT GCCAGAGGCT GGTAAATGGA TCAACGAGTG GAAAGAGCGT 
GTCAACTTCT TTCTCACAAA ATGTGACCAA TGTGCCAGGA CATGGCATAG GACACGCCAG 
CAATTCATCA GAGCCATTGA TCAATACACT TCCCGAGACA ACGCCGAGCT CTTGCGGAAT 
CTACAGAAAA ACCTCGAAGC CTTCGATGAA GAGCGCATCA CGCGCGGCCT CAAGAGAGCC 
AAATTCATCC TTGAGAGGGA CGGTCCCATG CCGACTACGA AGCTCGTTAG AATCGAACTT 
TGGCCGTGTC TCGCCTTGTT CGAATCGCTC TGCTGCATGG CATACTTGAA CACCAACAAA 
GCAAACCTAG AGATATTCAA TTACGTATTC GAACATGTCC AGCCTAAGAA GCCGCTGAAG 
ATGGTGGGAG GCGCAATTCC GACCATGACA TACTTCCTTT TCGAAGAGTA TCCGGTGCAC 
AGGGCGTTTG CAGAGGCACA ATGGCAACGG ATGCTGCCGG AAAGCCTCAC CGATGAGGAG 
TGGGAATGGG CTGTCAGAAG CGGCCTCAAG TCCCATATAC TCGGCCATAG GGGGATACAG 
CCCCCGGCGC GGCTGTGTCG CTTTTGGAGC GGATTCCTCC TCATTTTGCG AGCTATGAGC 
GAAAAGAGTA TTTTGGAAAA CCTCCGCGGT ATGGAAGTAC AACCGAATGT CTACTTCCTT 
GCTCTCGAAA ACCTCGGCAC CAATAACGTC CCTGCTTTGA GCATGATACT GAAAGCACTT 
CGTACGCTAA TGGAGCGTTC GTCAAAGGCT TTCTGGGACG CCTATGACCA AGTCACTCCG 
AGTATGATTG TGGAGGAAAT CTTCAAGAGC CCGGCTTTTC GGCCCTTTTT AAACCAGTCT 
CTTGAGCCGG ACATGATGGT CGAGGACCAG GAAAACAACG GTCAACTAAT ACCAGCGTTG 
GCAGAATGGG TCAGGGCCTT TATCCGTTCC CTTCCCTCAG CGCGGCGGTC AGACGTTTGC 
GAGACTCTTC TTCGGCATCT GTTCGAAACT CTCCGGGTCG ACGCAGGCGC AGCCCCAGAA 
GCGAAGGCCA CTTGCATCCT TGCTGGTCTG GTGACCTTGA GAGAATGCTT GGACGGCTAC 
CTCAGCCTTC CATCCTTTGA TACAGGAACG TCTCTTATTA TGGTCAACCA GTTACTCAAC 
AGAGTGATTC AGTACAAGGA AGTCATCATT GACGCGACCA GGCTCAAGGC CGCGGACAAG 
TACAACGTTG GCCTCTCACA GGCTGCTATA GCTATCATAC AGTCTGCCCT GGCATTGGAC 
GCGAAGGCGA CGTTGATGGA GTGGGACGCT TTCGTTAAGG AAAAGCCTGT TCAAGATGCC 
GTAAACAGGG ACTCTGGCGC ATTGTGGGAG TCATTCCTCG AGCTCGTCTG GAACGGACAA 
ACAGATCTCG CCAAGGCCAT GTTGAGAGCT ACGATGCCTC TCAGGAGTAT CGAACGGTTT 
ATCCCTAAGC GGAAAGACCA GCTCAAGAAC GAGCATGCAA AATTCAACAG ACGCTTTCAA 
CAGCAAACGG CGGCAATTGG CAAAATGTTA GGCCGCCTGT CTGACTTCAA CCCGGCTGAT 
CTTGACAGTT TCTGCTCGGA TCCGCAGAGT AACACCATCC AGCCCATCGT GGCGACTCTA 
ATACACGGTG AGGAAGCCAT TCGCGAAGCT GGCTTCGAGT TGATCAAAGC CATCACAGGT 
GAGATGACGC CATCTGAGGC CGTGAATAAG ATGCTTGAGG AGTACTTCAG CCAGTTCCTG 
GAGGCCTTCA CTGGCGCCGT AAACCATATC ACCAACGCCA AAGACTCCAA TAATCCGTGG 
AGTCACATGC AACACTTGTT GAAATGCTGT GAATTCGTGC TCAATAGCTT AAGTGATCCG 
TCTGTAGGTC AACTCAGACG CAAGACCCTG ACCCCTGCGG AACATGCTAT CGTCAAGGGA 
TGGTGGGATT GCGAATGGAG AGCCGTCGGC CACTCGTTCG GATCCATGAG ACTCTGGCAC 
GTGAAGGTGG ACAAGAAAGT CATGGAGGAC TTTTGTCGTG ATGTGATGGA ACTTGCAGAG 
AAGCTCCTCG CCCAAGACGG TCTCATGGCT TCGGCACTTA GCAGTCCTCA AAGCATCTCG 
GCGACAAACG GTGACGACGG CTCAGAGGGC ATGAAGTTGG TCCTGGAGCC GCCGAGAATA 
CACTCGCTTC CCTTAGTGGA CATGTTGCAA CTCCGCGATA AATATCTTAT CGCCGGTATT 
ATCGAAATCA CCAAGAAGTT GATCAAGCGT CTGCAAGACA ACGGCATGGA TCTTCCGCCA 
AAAACAGTCG CCTATCTCAA CAATATGCTG AAGAAGAGGA AGATGACTGA TGGGCGAACG 
GACTACCCCA TAAAGACGAA TCTCACCAAC GAGCAACGGA TCGAGCTACT CAAGGCGCTC 
GGGGACGATG CTGCCATCGA AGCGCAGTTC GTTGGTGGAA AGCCTGTGAA GCAGGAGGTC 
AAGCCGGTTA TGAAACAGGC AAAGCTTGAT TTCTCAAAGG CATCACTCAA AGACCACATT 
GCCTCGCTTA CGCCTGCCTT TGAGAAGGCC ACTGAGAGGC GGAAGAATGC TCTGTTGGAG 
ACGATGAAGG CTGAGGCTCC AGCCAAGGCT CCTCCCAAAA TCGACTCAAA GGCTGCCGAC 
AAGATCAAGG AGGCTAGGCG GCTTGAGCGA GAAGCCAAGG CCAAGCGAGA TGCTGAGGCC 
ATCGCGAGGG CAAAGGCCCT GCGTGCGCCA CCGAAGCTGG TCCAGGGAGA AGGCTCAGGT 
CTGGCTGGTG TTGCTGGAGT TCGTGGCAAA GACCACGCCC CAGTGCTGAA GGATGAGATC 
ATGGTTGGTT CATCATCGGA AGACGAAGAT GAGTCTGACG ATGACGAGTT GATCACACTC 
CATGCCGCTG GAAAGAAGAA TATGACTGAG GCCGAGCGTC GCGCCCATCG GTTGTTGGCT 
GAGAAGATGC GCGGACCGGT GAAGAAGGTC AAGATCGAGC GGTCCGCCAA GGATATGCGC 
GCTCGCCTTA TTCCCCCTAT GGATGTGTTG CACCAGGCCA TCTTGGAGTG GGACATCTTC 
CACGAAGGTA ACGATCCGCC GAACGGGTAC CGTTGTGATC ACGTCTCGGA TACCTACCAG 
GACCCGGTCA GCTACAAGCA AACCTTCTTT CCGTTGCTCA TCAATGAAGC CTGGCGCTCG 
TTTGTCACAG CCAAGGATGA AACCACGTCC AAGCCCTTTG GCATCAAGGT ACTTAGTCGC 
ATGACAGTCG ACAAGTTCAT GGAAGTTACG GCGTCGGTTC CAGTTGCAAT CAACAAAGAC 
CGTCAATTGT CTGAAGGAGA TATTGTCATC ATTTCCAAGG GCCAAAACCC GCTTCAAGAG 
CCAGAAGAGC TACACTGTCT TTCGCGTATC TGGAAGACAA CTTACAAAAA AGACAATGTG 
GAGGTCGTCT ACCGCCTGAA TGCCAAAGGG AACCAAATTC TACCTGCGCT ACTGCCTGGC 
TCGGAGTTTA CCGTTGTCAA GATCACCAAC ATGACGACCA TTGAACGCGA GTATGCCGCC 
CTGGAAAGTT TACAGTACTA TGATCTTATG GACGAGGTAT TGAGGGCTGA ACCGTCTCCT 
ATGCTGTCTT TTGGCGAGGA GAACATCAAG AACACCATGA AAAACTGGAA TCTTAACCCA 
GGTCAAGCGA AGGCGATCCT GAACGCCAAG GAGAACGATG GGTTCACCCT TATCCAGGGT 
CCTCCAGGTA CCGGTAAAAC TAAGACCATT GTGGCAATGG TTGGCTGTCT TCTGACCGGC 
GTGCTCAAAA ACCCTACCGC TGGGGTGGCC ATTGGGCGTC CGGGACTCGG AGCTGCGAAG 
AACAACGCTC CAGCAAAGAA GCTGCTTGTT TGCGCGCCCA GTAATGCCGC TGTGGACGAA 
TTGGTGTTGC GCTTGAAGAA TGGTGTCAAG ACCCAGAACG GAACAACTCA CCAAATTGAG 
GTGGTCCGTC TCGGTCGCAG TGATGCCATC AACAGCGCAG TCAAGGATGT CACGCTTGAT 
GAGCTGGTCA AGGCGAAACT CGAGGCTCAG TTGAACAAGG ATGAAGGCCC GACTGACCGG 
GAGAAGCTGC ACCAAGAAGC GGGCGAGATC AAGCAGAAGA TTGCCGAACT CAGACCTCAA 
CTGGAAGCAG CGCGCACGAT TGACGACAGG CAGCTGGTCA ACAAATATCA GCGGGAGTTC 
GACGAACTCA AGCGGCGCCA GGCGCACATC GGAGCGCGCA TCGATGCGGA TAAAGCTAGC 
GGCAACACAT TTGCTAGAGA GACCGAAATC AAACGCCGAC AGGTCCAGCA GGAGATTCTG 
GACAAGGCTC AGGTGCTGTG CGCCACTCTC AGCGGTAGCG GTCACGAGAT GTTCAAGAAC 
TTGAACGTGG AGTTTGAGAC GGTCATCATC GATGAGGCGG CGCAGTGTGT GGAGCTCAGT 
GCGCTCATTC CCCTGAAGTA CGGATGCTCC AAGTGCATTC TTGTCGGTGA TCCTAAGCAG 
TTACCACCTA CCGTTCTCTC GCAGTCCGCT GCACGGTACG GCTACGACCA GAGTTTGTTC 
GTCCGCATGC AGAAGAATCA CGAGAAGGAC GTACATCTCC TCGACACCCA GTATCGTATG 
CATCCTGAAA TCAGCTCCTT CCCCAGAGCC GCCTTTTACG AAGGTCTCCT ACAAGACGGT 
GACGACATGG CCAAATCGCG CCTCCAGCCC TGGCACCGCA GTACTTTGCT TGGTCCGTAC 
CGCTTCTTCG ACGTCCGCGG CTCGCAGGAG CGGGGACCAA AGAATCAGTC TCTGGTCAAC 
GAAGAAGAAC TCAAGGTCGC CATGCAACTT TATCGCCGTT TCAAGGCCGA TTACGGCAAT 
GTCGACCTCA AGGGCAAGAT CGGTATTATC ACTCCTTACA AGGCCCAGCT GTACCGGCTG 
CGGTCTCAGT TTGCCCAGCG CTTCGGCGAC GCCATCACGG ACGAAATCGA GTTCAATACC 
ACTGATGCCT TCCAAGGACG AGAGTGCGAG ATCATTATTT TCTCATGCGT TCGTGCCAGC 
CCCACAGGTG GTATCGGTTT CATGACAGAT ATCCGTCGTA TGAACGTCGG TCTCACGCGT 
GCGCGGTCTT CACTGTGGAT TCTCGGTGAT TCCCGCGCTC TCATGCAGGG TGAGTTCTGG 
GCGAAACTCA TTGAGGATTC TAAGCGGCGC GATAGGTATA CCACGGGAAA TATCATGGGC 
ATGCTAAGCC AGGCGGGGCC ACAGCTGTCT GCTGCGGCGT TCGAGGCTCT GGCGATTACT 
AGCACTGGTA ATACCAGGGA AAGTACGCCA AGCGGTATCA AGCGGGAGGT GGTCGAGGTG 
AAGGACGAGG ATAGCGATGT GCAAATGGGG GATCGATCGT CCTTTAGGGG GTCTTCTCCT 
CCGGCTGCTG CTGTTGGCGG CAGCAGTGCG ATGGGTTCGT CCAGACCTCC CTCCGGACGC 
AATACTCCAA GTCATACCGG GAACGGGTTC GGAAGTTCGC CTGCCCCGCA GCAGCCGCAG 
CCAACACAGT ACAGGGAAGG CTTGCCGGTC ATTCAGGAGA CGACTTTCAC GCCGAGGGTA 
AAGAAGAGGC CGTATGAGGG AGGAGGGGGT GATCAACCTG GCAAGAAGCA ACACATCGAC 
CCCATCGCCA GCAGAGCCCC CAAGGGGCCC AGGGGTTACA ACCCCAATAC CAACAACCTT 
CCACCAAGAC CACCGCCGCA GGCGCCGAGT GACCCCTCAG CGATGGAAGT TCTGGGGTTG 
GCACCCCCTA CCAGAGCACC GGCTGCTCGG CAGCAGCAGC AGCAGCAACC GCAGTACGAC 
TATCAACCTC AGCAGCCTCC TCAATACCAA CCTCGTCCTC AACAAGGAGC TCCGCCTCCG 
TATCCGGGCC ATCAGCAACA GCGACAACCG CCTGCTGGTC CTTCTCGACC CGGTCCTCCA 
ACTGGACCAA GCAGACTGGG TGGTAGTGGT ACAGGTTACC AGCAGCAGCA GCAACAACAA 
CCGCAACAGC AACAACAACA ACAACAGAGA CCGCCGCCGA TAGTAAAGAA GAAGGCGCCT 
GTTGATCCGT TCATCAAGCG GAAGAAGAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_964658.3
CDS	547..6939
Translation	MSDKMEYPELGTADVQMAMMEMHQAFEEFPPDAHPICPLLSDDDDTDYALPDLPDANGTTVAEKQKRIADGFERRRITYGLSLLLVMPEAGKWINEWKERVNFFLTKCDQCARTWHRTRQQFIRAIDQYTSRDNAELLRNLQKNLEAFDEERITRGLKRAKFILERDGPMPTTKLVRIELWPCLALFESLCCMAYLNTNKANLEIFNYVFEHVQPKKPLKMVGGAIPTMTYFLFEEYPVHRAFAEAQWQRMLPESLTDEEWEWAVRSGLKSHILGHRGIQPPARLCRFWSGFLLILRAMSEKSILENLRGMEVQPNVYFLALENLGTNNVPALSMILKALRTLMERSSKAFWDAYDQVTPSMIVEEIFKSPAFRPFLNQSLEPDMMVEDQENNGQLIPALAEWVRAFIRSLPSARRSDVCETLLRHLFETLRVDAGAAPEAKATCILAGLVTLRECLDGYLSLPSFDTGTSLIMVNQLLNRVIQYKEVIIDATRLKAADKYNVGLSQAAIAIIQSALALDAKATLMEWDAFVKEKPVQDAVNRDSGALWESFLELVWNGQTDLAKAMLRATMPLRSIERFIPKRKDQLKNEHAKFNRRFQQQTAAIGKMLGRLSDFNPADLDSFCSDPQSNTIQPIVATLIHGEEAIREAGFELIKAITGEMTPSEAVNKMLEEYFSQFLEAFTGAVNHITNAKDSNNPWSHMQHLLKCCEFVLNSLSDPSVGQLRRKTLTPAEHAIVKGWWDCEWRAVGHSFGSMRLWHVKVDKKVMEDFCRDVMELAEKLLAQDGLMASALSSPQSISATNGDDGSEGMKLVLEPPRIHSLPLVDMLQLRDKYLIAGIIEITKKLIKRLQDNGMDLPPKTVAYLNNMLKKRKMTDGRTDYPIKTNLTNEQRIELLKALGDDAAIEAQFVGGKPVKQEVKPVMKQAKLDFSKASLKDHIASLTPAFEKATERRKNALLETMKAEAPAKAPPKIDSKAADKIKEARRLEREAKAKRDAEAIARAKALRAPPKLVQGEGSGLAGVAGVRGKDHAPVLKDEIMVGSSSEDEDESDDDELITLHAAGKKNMTEAERRAHRLLAEKMRGPVKKVKIERSAKDMRARLIPPMDVLHQAILEWDIFHEGNDPPNGYRCDHVSDTYQDPVSYKQTFFPLLINEAWRSFVTAKDETTSKPFGIKVLSRMTVDKFMEVTASVPVAINKDRQLSEGDIVIISKGQNPLQEPEELHCLSRIWKTTYKKDNVEVVYRLNAKGNQILPALLPGSEFTVVKITNMTTIEREYAALESLQYYDLMDEVLRAEPSPMLSFGEENIKNTMKNWNLNPGQAKAILNAKENDGFTLIQGPPGTGKTKTIVAMVGCLLTGVLKNPTAGVAIGRPGLGAAKNNAPAKKLLVCAPSNAAVDELVLRLKNGVKTQNGTTHQIEVVRLGRSDAINSAVKDVTLDELVKAKLEAQLNKDEGPTDREKLHQEAGEIKQKIAELRPQLEAARTIDDRQLVNKYQREFDELKRRQAHIGARIDADKASGNTFARETEIKRRQVQQEILDKAQVLCATLSGSGHEMFKNLNVEFETVIIDEAAQCVELSALIPLKYGCSKCILVGDPKQLPPTVLSQSAARYGYDQSLFVRMQKNHEKDVHLLDTQYRMHPEISSFPRAAFYEGLLQDGDDMAKSRLQPWHRSTLLGPYRFFDVRGSQERGPKNQSLVNEEELKVAMQLYRRFKADYGNVDLKGKIGIITPYKAQLYRLRSQFAQRFGDAITDEIEFNTTDAFQGRECEIIIFSCVRASPTGGIGFMTDIRRMNVGLTRARSSLWILGDSRALMQGEFWAKLIEDSKRRDRYTTGNIMGMLSQAGPQLSAAAFEALAITSTGNTRESTPSGIKREVVEVKDEDSDVQMGDRSSFRGSSPPAAAVGGSSAMGSSRPPSGRNTPSHTGNGFGSSPAPQQPQPTQYREGLPVIQETTFTPRVKKRPYEGGGGDQPGKKQHIDPIASRAPKGPRGYNPNTNNLPPRPPPQAPSDPSAMEVLGLAPPTRAPAARQQQQQQPQYDYQPQQPPQYQPRPQQGAPPPYPGHQQQRQPPAGPSRPGPPTGPSRLGGSGTGYQQQQQQQPQQQQQQQQRPPPIVKKKAPVDPFIKRKKR

Target ORF information:

RefSeq Version	XM_959565.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A tRNA-splicing endonuclease (NCU02036), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_959565.3

ORF Insert Sequence:

ATGTCAGACA AAATGGAATA CCCAGAGCTG GGGACCGCGG ACGTTCAGAT GGCTATGATG 
GAGATGCATC AAGCCTTTGA AGAGTTTCCT CCAGACGCAC ATCCAATTTG CCCTTTGCTC 
AGCGACGATG ACGATACGGA CTACGCGTTG CCAGACCTCC CCGATGCGAA CGGAACAACG 
GTGGCAGAAA AGCAAAAGCG AATCGCTGAC GGGTTCGAGA GACGTAGAAT TACCTACGGC 
CTTTCGCTTC TGTTGGTGAT GCCAGAGGCT GGTAAATGGA TCAACGAGTG GAAAGAGCGT 
GTCAACTTCT TTCTCACAAA ATGTGACCAA TGTGCCAGGA CATGGCATAG GACACGCCAG 
CAATTCATCA GAGCCATTGA TCAATACACT TCCCGAGACA ACGCCGAGCT CTTGCGGAAT 
CTACAGAAAA ACCTCGAAGC CTTCGATGAA GAGCGCATCA CGCGCGGCCT CAAGAGAGCC 
AAATTCATCC TTGAGAGGGA CGGTCCCATG CCGACTACGA AGCTCGTTAG AATCGAACTT 
TGGCCGTGTC TCGCCTTGTT CGAATCGCTC TGCTGCATGG CATACTTGAA CACCAACAAA 
GCAAACCTAG AGATATTCAA TTACGTATTC GAACATGTCC AGCCTAAGAA GCCGCTGAAG 
ATGGTGGGAG GCGCAATTCC GACCATGACA TACTTCCTTT TCGAAGAGTA TCCGGTGCAC 
AGGGCGTTTG CAGAGGCACA ATGGCAACGG ATGCTGCCGG AAAGCCTCAC CGATGAGGAG 
TGGGAATGGG CTGTCAGAAG CGGCCTCAAG TCCCATATAC TCGGCCATAG GGGGATACAG 
CCCCCGGCGC GGCTGTGTCG CTTTTGGAGC GGATTCCTCC TCATTTTGCG AGCTATGAGC 
GAAAAGAGTA TTTTGGAAAA CCTCCGCGGT ATGGAAGTAC AACCGAATGT CTACTTCCTT 
GCTCTCGAAA ACCTCGGCAC CAATAACGTC CCTGCTTTGA GCATGATACT GAAAGCACTT 
CGTACGCTAA TGGAGCGTTC GTCAAAGGCT TTCTGGGACG CCTATGACCA AGTCACTCCG 
AGTATGATTG TGGAGGAAAT CTTCAAGAGC CCGGCTTTTC GGCCCTTTTT AAACCAGTCT 
CTTGAGCCGG ACATGATGGT CGAGGACCAG GAAAACAACG GTCAACTAAT ACCAGCGTTG 
GCAGAATGGG TCAGGGCCTT TATCCGTTCC CTTCCCTCAG CGCGGCGGTC AGACGTTTGC 
GAGACTCTTC TTCGGCATCT GTTCGAAACT CTCCGGGTCG ACGCAGGCGC AGCCCCAGAA 
GCGAAGGCCA CTTGCATCCT TGCTGGTCTG GTGACCTTGA GAGAATGCTT GGACGGCTAC 
CTCAGCCTTC CATCCTTTGA TACAGGAACG TCTCTTATTA TGGTCAACCA GTTACTCAAC 
AGAGTGATTC AGTACAAGGA AGTCATCATT GACGCGACCA GGCTCAAGGC CGCGGACAAG 
TACAACGTTG GCCTCTCACA GGCTGCTATA GCTATCATAC AGTCTGCCCT GGCATTGGAC 
GCGAAGGCGA CGTTGATGGA GTGGGACGCT TTCGTTAAGG AAAAGCCTGT TCAAGATGCC 
GTAAACAGGG ACTCTGGCGC ATTGTGGGAG TCATTCCTCG AGCTCGTCTG GAACGGACAA 
ACAGATCTCG CCAAGGCCAT GTTGAGAGCT ACGATGCCTC TCAGGAGTAT CGAACGGTTT 
ATCCCTAAGC GGAAAGACCA GCTCAAGAAC GAGCATGCAA AATTCAACAG ACGCTTTCAA 
CAGCAAACGG CGGCAATTGG CAAAATGTTA GGCCGCCTGT CTGACTTCAA CCCGGCTGAT 
CTTGACAGTT TCTGCTCGGA TCCGCAGAGT AACACCATCC AGCCCATCGT GGCGACTCTA 
ATACACGGTG AGGAAGCCAT TCGCGAAGCT GGCTTCGAGT TGATCAAAGC CATCACAGGT 
GAGATGACGC CATCTGAGGC CGTGAATAAG ATGCTTGAGG AGTACTTCAG CCAGTTCCTG 
GAGGCCTTCA CTGGCGCCGT AAACCATATC ACCAACGCCA AAGACTCCAA TAATCCGTGG 
AGTCACATGC AACACTTGTT GAAATGCTGT GAATTCGTGC TCAATAGCTT AAGTGATCCG 
TCTGTAGGTC AACTCAGACG CAAGACCCTG ACCCCTGCGG AACATGCTAT CGTCAAGGGA 
TGGTGGGATT GCGAATGGAG AGCCGTCGGC CACTCGTTCG GATCCATGAG ACTCTGGCAC 
GTGAAGGTGG ACAAGAAAGT CATGGAGGAC TTTTGTCGTG ATGTGATGGA ACTTGCAGAG 
AAGCTCCTCG CCCAAGACGG TCTCATGGCT TCGGCACTTA GCAGTCCTCA AAGCATCTCG 
GCGACAAACG GTGACGACGG CTCAGAGGGC ATGAAGTTGG TCCTGGAGCC GCCGAGAATA 
CACTCGCTTC CCTTAGTGGA CATGTTGCAA CTCCGCGATA AATATCTTAT CGCCGGTATT 
ATCGAAATCA CCAAGAAGTT GATCAAGCGT CTGCAAGACA ACGGCATGGA TCTTCCGCCA 
AAAACAGTCG CCTATCTCAA CAATATGCTG AAGAAGAGGA AGATGACTGA TGGGCGAACG 
GACTACCCCA TAAAGACGAA TCTCACCAAC GAGCAACGGA TCGAGCTACT CAAGGCGCTC 
GGGGACGATG CTGCCATCGA AGCGCAGTTC GTTGGTGGAA AGCCTGTGAA GCAGGAGGTC 
AAGCCGGTTA TGAAACAGGC AAAGCTTGAT TTCTCAAAGG CATCACTCAA AGACCACATT 
GCCTCGCTTA CGCCTGCCTT TGAGAAGGCC ACTGAGAGGC GGAAGAATGC TCTGTTGGAG 
ACGATGAAGG CTGAGGCTCC AGCCAAGGCT CCTCCCAAAA TCGACTCAAA GGCTGCCGAC 
AAGATCAAGG AGGCTAGGCG GCTTGAGCGA GAAGCCAAGG CCAAGCGAGA TGCTGAGGCC 
ATCGCGAGGG CAAAGGCCCT GCGTGCGCCA CCGAAGCTGG TCCAGGGAGA AGGCTCAGGT 
CTGGCTGGTG TTGCTGGAGT TCGTGGCAAA GACCACGCCC CAGTGCTGAA GGATGAGATC 
ATGGTTGGTT CATCATCGGA AGACGAAGAT GAGTCTGACG ATGACGAGTT GATCACACTC 
CATGCCGCTG GAAAGAAGAA TATGACTGAG GCCGAGCGTC GCGCCCATCG GTTGTTGGCT 
GAGAAGATGC GCGGACCGGT GAAGAAGGTC AAGATCGAGC GGTCCGCCAA GGATATGCGC 
GCTCGCCTTA TTCCCCCTAT GGATGTGTTG CACCAGGCCA TCTTGGAGTG GGACATCTTC 
CACGAAGGTA ACGATCCGCC GAACGGGTAC CGTTGTGATC ACGTCTCGGA TACCTACCAG 
GACCCGGTCA GCTACAAGCA AACCTTCTTT CCGTTGCTCA TCAATGAAGC CTGGCGCTCG 
TTTGTCACAG CCAAGGATGA AACCACGTCC AAGCCCTTTG GCATCAAGGT ACTTAGTCGC 
ATGACAGTCG ACAAGTTCAT GGAAGTTACG GCGTCGGTTC CAGTTGCAAT CAACAAAGAC 
CGTCAATTGT CTGAAGGAGA TATTGTCATC ATTTCCAAGG GCCAAAACCC GCTTCAAGAG 
CCAGAAGAGC TACACTGTCT TTCGCGTATC TGGAAGACAA CTTACAAAAA AGACAATGTG 
GAGGTCGTCT ACCGCCTGAA TGCCAAAGGG AACCAAATTC TACCTGCGCT ACTGCCTGGC 
TCGGAGTTTA CCGTTGTCAA GATCACCAAC ATGACGACCA TTGAACGCGA GTATGCCGCC 
CTGGAAAGTT TACAGTACTA TGATCTTATG GACGAGGTAT TGAGGGCTGA ACCGTCTCCT 
ATGCTGTCTT TTGGCGAGGA GAACATCAAG AACACCATGA AAAACTGGAA TCTTAACCCA 
GGTCAAGCGA AGGCGATCCT GAACGCCAAG GAGAACGATG GGTTCACCCT TATCCAGGGT 
CCTCCAGGTA CCGGTAAAAC TAAGACCATT GTGGCAATGG TTGGCTGTCT TCTGACCGGC 
GTGCTCAAAA ACCCTACCGC TGGGGTGGCC ATTGGGCGTC CGGGACTCGG AGCTGCGAAG 
AACAACGCTC CAGCAAAGAA GCTGCTTGTT TGCGCGCCCA GTAATGCCGC TGTGGACGAA 
TTGGTGTTGC GCTTGAAGAA TGGTGTCAAG ACCCAGAACG GAACAACTCA CCAAATTGAG 
GTGGTCCGTC TCGGTCGCAG TGATGCCATC AACAGCGCAG TCAAGGATGT CACGCTTGAT 
GAGCTGGTCA AGGCGAAACT CGAGGCTCAG TTGAACAAGG ATGAAGGCCC GACTGACCGG 
GAGAAGCTGC ACCAAGAAGC GGGCGAGATC AAGCAGAAGA TTGCCGAACT CAGACCTCAA 
CTGGAAGCAG CGCGCACGAT TGACGACAGG CAGCTGGTCA ACAAATATCA GCGGGAGTTC 
GACGAACTCA AGCGGCGCCA GGCGCACATC GGAGCGCGCA TCGATGCGGA TAAAGCTAGC 
GGCAACACAT TTGCTAGAGA GACCGAAATC AAACGCCGAC AGGTCCAGCA GGAGATTCTG 
GACAAGGCTC AGGTGCTGTG CGCCACTCTC AGCGGTAGCG GTCACGAGAT GTTCAAGAAC 
TTGAACGTGG AGTTTGAGAC GGTCATCATC GATGAGGCGG CGCAGTGTGT GGAGCTCAGT 
GCGCTCATTC CCCTGAAGTA CGGATGCTCC AAGTGCATTC TTGTCGGTGA TCCTAAGCAG 
TTACCACCTA CCGTTCTCTC GCAGTCCGCT GCACGGTACG GCTACGACCA GAGTTTGTTC 
GTCCGCATGC AGAAGAATCA CGAGAAGGAC GTACATCTCC TCGACACCCA GTATCGTATG 
CATCCTGAAA TCAGCTCCTT CCCCAGAGCC GCCTTTTACG AAGGTCTCCT ACAAGACGGT 
GACGACATGG CCAAATCGCG CCTCCAGCCC TGGCACCGCA GTACTTTGCT TGGTCCGTAC 
CGCTTCTTCG ACGTCCGCGG CTCGCAGGAG CGGGGACCAA AGAATCAGTC TCTGGTCAAC 
GAAGAAGAAC TCAAGGTCGC CATGCAACTT TATCGCCGTT TCAAGGCCGA TTACGGCAAT 
GTCGACCTCA AGGGCAAGAT CGGTATTATC ACTCCTTACA AGGCCCAGCT GTACCGGCTG 
CGGTCTCAGT TTGCCCAGCG CTTCGGCGAC GCCATCACGG ACGAAATCGA GTTCAATACC 
ACTGATGCCT TCCAAGGACG AGAGTGCGAG ATCATTATTT TCTCATGCGT TCGTGCCAGC 
CCCACAGGTG GTATCGGTTT CATGACAGAT ATCCGTCGTA TGAACGTCGG TCTCACGCGT 
GCGCGGTCTT CACTGTGGAT TCTCGGTGAT TCCCGCGCTC TCATGCAGGG TGAGTTCTGG 
GCGAAACTCA TTGAGGATTC TAAGCGGCGC GATAGGTATA CCACGGGAAA TATCATGGGC 
ATGCTAAGCC AGGCGGGGCC ACAGCTGTCT GCTGCGGCGT TCGAGGCTCT GGCGATTACT 
AGCACTGGTA ATACCAGGGA AAGTACGCCA AGCGGTATCA AGCGGGAGGT GGTCGAGGTG 
AAGGACGAGG ATAGCGATGT GCAAATGGGG GATCGATCGT CCTTTAGGGG GTCTTCTCCT 
CCGGCTGCTG CTGTTGGCGG CAGCAGTGCG ATGGGTTCGT CCAGACCTCC CTCCGGACGC 
AATACTCCAA GTCATACCGG GAACGGGTTC GGAAGTTCGC CTGCCCCGCA GCAGCCGCAG 
CCAACACAGT ACAGGGAAGG CTTGCCGGTC ATTCAGGAGA CGACTTTCAC GCCGAGGGTA 
AAGAAGAGGC CGTATGAGGG AGGAGGGGGT GATCAACCTG GCAAGAAGCA ACACATCGAC 
CCCATCGCCA GCAGAGCCCC CAAGGGGCCC AGGGGTTACA ACCCCAATAC CAACAACCTT 
CCACCAAGAC CACCGCCGCA GGCGCCGAGT GACCCCTCAG CGATGGAAGT TCTGGGGTTG 
GCACCCCCTA CCAGAGCACC GGCTGCTCGG CAGCAGCAGC AGCAGCAACC GCAGTACGAC 
TATCAACCTC AGCAGCCTCC TCAATACCAA CCTCGTCCTC AACAAGGAGC TCCGCCTCCG 
TATCCGGGCC ATCAGCAACA GCGACAACCG CCTGCTGGTC CTTCTCGACC CGGTCCTCCA 
ACTGGACCAA GCAGACTGGG TGGTAGTGGT ACAGGTTACC AGCAGCAGCA GCAACAACAA 
CCGCAACAGC AACAACAACA ACAACAGAGA CCGCCGCCGA TAGTAAAGAA GAAGGCGCCT 
GTTGATCCGT TCATCAAGCG GAAGAAGAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU02036 cDNA ORF clone, Neurospora crassa OR74A

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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