NCU09318 cDNA ORF clone, Neurospora crassa OR74A

The following NCU09318 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU09318 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf02483 XM_958929.3
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Neurospora crassa OR74A ATP-dependent DNA helicase mph-1 (NCU09318), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf02483
    Clone ID Related Accession (Same CDS sequence) XM_958929.3
    Accession Version XM_958929.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product ATP-dependent DNA helicase mph-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_958929.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATGACG ATGACTTTGA CGATATTCCT GATGAGGATT TGATGTTGGC CTTCACCCAG 
    GCCACCGGCA ACATCACATC ACATCACCCC AGCAACAGCA AGCAGCTTGC CTCGTCGGCA
    AAACCACCAG CACAAGCGTG GCCAATATCC GATTCTGCAA GACGAATCGT TACTCCAACC
    GTATCGCAGG GTCAGGCTAC AGCTACAGGG CGTGCCAAAA CAGCAAGCAA GCCGACCACG
    TCAGCGACAA CGTCTCGCCC GTCCCTAGCT CAGTCCAGCC AGCGTAAGAA TCTGCGTCAG
    ACGACACTAT GGGGTGGCAC GCTGGAGGAG GATGCGCAAC CCGCCCCGCA AGCGGTGTCG
    AATCGACCAT TCCGTGCCGA TATGCCCCCA GAGCAGCCCA CTCACCATGA GATTGACATA
    GAAGAAATGA AAACATGGGT TTATCCGATG AATCTGGGAC CTATTCGAGA CTACCAGTTC
    AGCATTGTCA AAAATGGCCT CTTCAACAAC ACATTGGTCG CCCTCCCCAC GGGTTTGGGA
    AAGACATTTA TTGCTGCGAC AATCATGCTA AACTACATCC GCTGGACAAA AACCGCAAAG
    GCTGTCTTTG TTGCTCCTAC GAAACCCCTT GCTTCACAAC AAGTTCAAGC ATGTCTCAGC
    ATAGCGGGCA TCCCGCGATC TCAGGCGACT CTCCTAACCG GAGAGACTCC TCCTGTTCTA
    CGTGAGGACG AATGGGCAAC AAAGCGCCTG TTCTTCATGA CACCCCAAAC ACTAATGAAT
    GACCTTTCCA AGGGCTATGC CGATCCCAAG TCCATCGTAC TGCTGGTTAT TGACGAGGCG
    CATCGTGCGA CTGGAGACTA TGCATACGTC AAGGTGGTCG AATTTTTAAG GCGTTTCTCC
    AAGAGCTTTC GAATTCTTGC TCTGACTGCT ACACCAGGAT CGTCTCTCGA AGGTGTTCAG
    GACGTCATTG ATAATCTGGG CATTTCTCAC GTCGAGATCA GAACCGAAGA GTCAATCGAC
    ATTCGTCAAT ATGTGCACTC AAGAGACATC AACACGATAA CATTCGACCC CTCTGACGAA
    ATGATGGAAG TCAGAGACTT GTTCTCCAAG GCCCTGAAGC CCCTTGTGAC CAAGCTGAGT
    TCACAAAATA TATACTACGG CAGGGACCCC ATGAGCCTGA CTACCTACGG CCTGATGAAA
    GCCAGGAATG ACTGGATGGC AGGTCCGGGT AGGCACGTAA ACCAAGGAAC CAAATTCAGT
    GTCATAGCCA CATTTGCGAT TCTCCAGAGT TTGGCGCATT CCATCAAACT ACTCAACTTC
    CATGGAATCA AACCGTTCTA CAATAATCTG GCCGAATTCC GCACCACCGA GGAGGAGAAA
    GGAGGAAAAG GGTCAAAGCT CAAGCGGCAA GTCTTGGAGG ATGAGAACTT TCAGAAGATG
    ATGGACATGA TTGAGGGTTG GATGAAGATT GATGGGTTCC TCGGACACCC CAAGCTGGAA
    TATCTTTGTG AGACCCTGGT CAACCACTTC ATGGATGCTG GCGAGGGCTC CAATACCAGA
    GCCATTGTAT TCAGTGAATA CCGAGACAGT GCCGAAGAGA TTGTGCGCAT TCTCAACAAG
    CAACCCCTTA TCAAGGCCAC AGTCTTCGTT GGACAAGCGG ACTCGAAGAG GAGTGAGGGC
    ATGAAACAGA AACAACAAAT TGAGACGATC GAGAAGTTCA AGAACGGCGC TCACAACGTT
    CTGGTCGCAA CATCCATTGG CGAGGAGGGT CTTGATATTG GCCAAGTGGA TCTCATTGTC
    TGCTACGATG CATCAGCGTC ACCGATCCGA ATGCTTCAGC GTATGGGTCG TACCGGCCGC
    AAGAGAGCGG GTAACATTGT ACTTTTGTTG ATGAAAGGGA AAGAGGAAGA CAAATTCAAC
    GAGGCGAAGG ACAACTACGC GACCATGCAG AGAATGATAT GCGAGGGCAG CCGCTTTACC
    TTTCGTCACG ACCTTTCTTC ACGTATTGTG CCACGAGACA TACGACCTGA GGTGGAAAAG
    AAGGTTGTGG AGATTCCACT TGAGAACTCC CAAAATCCAG AGCTCCCAGA ACCAAAAAGA
    TCTGCCGCAA GAATGAGGAC AAAGCCGGCA AAGAAGAAGT TCAACATGCC TGACGGTGTT
    GAAACAGGGT TCATCAAGGC TTCTTTCTTT GGTCAAGCTG GAGCTAAGAC AGCCAAACCA
    CCCGCTAGGC CACCTGCGCC TAAGGAAACT GACTTTATTG CCGAACGGCC CAAACTCGAA
    AGCATTTTGC TGAGCACGTC GCAGGAAAAC GAGCTCCGCA GAAACTATAC AAAGATACCC
    CTCGGACACT CGAAGGTTGA AGAGTTGGAT ATAGACTGGT ATCGCCATCC AACATCCAGA
    CGAGTGGTCC AGAAAACCAT CCATGTCAAA CACGGGGAAT ATACCAAGCG ATGTGTGAAA
    CTGTTTAGGA GCCTGGCAAA GTCTCAAGCT CCTGCGAATA GGTATACTAA GCCATACGGT
    GAAACAGACA CGTCCTCCTG GGAATTGATA CCATTGCCGC CCCTTGCGGA CGAAACAGAA
    GGAGAAACGT CACGAAAGGG GCAAAAGAAG AGGCCGCGCC TCGAGAGTGG GCAGGAAGCA
    GAGGAAGCAG AGCAATATGC CGCACCTAAG AAACGTCAAG CGACAGCAAA AACCAAATCT
    ACCGGGGTCT CCAAACAGAC CAACAAGCCT CGAGCACGAC ATACCGCACT TATCAGCGAC
    TGTGAAGAGG GTGGCAACGA ATATGATGGC AATGTCGACG ATGACGAACA AAGTAGGCCC
    CGAAACTTCC GCAGCAAGGG TCGTGGACGT GGCAGCGGGC GGGGAAAAAA ATCTCAACCT
    AAACAAGGAG ACCCCAACGT GGACTACGGT GACGACTGCA CCCGCACCAG CGACATGGAA
    ATGGGTACCG ATGGCTCTGA TGACGGTGCC GACCTCGAGG ACTTTATCGT CAGCGATGGC
    GAAGTCACCT CGAGTTTACA GCATCGTCCC CGAGGGTCCA CCTCGCCGAC AACAGCTCCT
    GACGCAGGGT CGTCTTCCTT GTCCTCGAAA ACGGGACGCA AGCAGCAAGC ACCAGATTCC
    TTCGCGTCGG ATGAAGATGA TGGCGATGTG TTTGGACCCA AATTTGTACC GGTAACCGCG
    TCGGCAGCTA AAGGCTCGTT GCCATCAACA GCGAGACGAG AGAAGCCCAA GCCGTTTTAT
    GTGCCGGTAG AATTACCTGC AACCCAGGAC ACAACGGACG GAGACGACGA CTTACCCGAT
    ATCGAATTTC TGTCTGCTAA GAGAAAGAGG GAAGGCACAG GTACGGGGAT GCGAACAGGG
    AGCCCAGGAC ATGTTAAAGT TGGCGACACC TCGAAAGGGG GCAGCGGCGG AGATCAAACT
    CGAGAGAAAC CTTCTGGTGG TGCGGCGTCC CATGCCCGAG CGAGGAAGCG AACTGTGGTG
    ATGGATAGTG ATGATGACCA GGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_964022.1
    CDS258..3764
    Translation

    Target ORF information:

    RefSeq Version XM_958929.3
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A ATP-dependent DNA helicase mph-1 (NCU09318), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_958929.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATGACG ATGACTTTGA CGATATTCCT GATGAGGATT TGATGTTGGC CTTCACCCAG 
    GCCACCGGCA ACATCACATC ACATCACCCC AGCAACAGCA AGCAGCTTGC CTCGTCGGCA
    AAACCACCAG CACAAGCGTG GCCAATATCC GATTCTGCAA GACGAATCGT TACTCCAACC
    GTATCGCAGG GTCAGGCTAC AGCTACAGGG CGTGCCAAAA CAGCAAGCAA GCCGACCACG
    TCAGCGACAA CGTCTCGCCC GTCCCTAGCT CAGTCCAGCC AGCGTAAGAA TCTGCGTCAG
    ACGACACTAT GGGGTGGCAC GCTGGAGGAG GATGCGCAAC CCGCCCCGCA AGCGGTGTCG
    AATCGACCAT TCCGTGCCGA TATGCCCCCA GAGCAGCCCA CTCACCATGA GATTGACATA
    GAAGAAATGA AAACATGGGT TTATCCGATG AATCTGGGAC CTATTCGAGA CTACCAGTTC
    AGCATTGTCA AAAATGGCCT CTTCAACAAC ACATTGGTCG CCCTCCCCAC GGGTTTGGGA
    AAGACATTTA TTGCTGCGAC AATCATGCTA AACTACATCC GCTGGACAAA AACCGCAAAG
    GCTGTCTTTG TTGCTCCTAC GAAACCCCTT GCTTCACAAC AAGTTCAAGC ATGTCTCAGC
    ATAGCGGGCA TCCCGCGATC TCAGGCGACT CTCCTAACCG GAGAGACTCC TCCTGTTCTA
    CGTGAGGACG AATGGGCAAC AAAGCGCCTG TTCTTCATGA CACCCCAAAC ACTAATGAAT
    GACCTTTCCA AGGGCTATGC CGATCCCAAG TCCATCGTAC TGCTGGTTAT TGACGAGGCG
    CATCGTGCGA CTGGAGACTA TGCATACGTC AAGGTGGTCG AATTTTTAAG GCGTTTCTCC
    AAGAGCTTTC GAATTCTTGC TCTGACTGCT ACACCAGGAT CGTCTCTCGA AGGTGTTCAG
    GACGTCATTG ATAATCTGGG CATTTCTCAC GTCGAGATCA GAACCGAAGA GTCAATCGAC
    ATTCGTCAAT ATGTGCACTC AAGAGACATC AACACGATAA CATTCGACCC CTCTGACGAA
    ATGATGGAAG TCAGAGACTT GTTCTCCAAG GCCCTGAAGC CCCTTGTGAC CAAGCTGAGT
    TCACAAAATA TATACTACGG CAGGGACCCC ATGAGCCTGA CTACCTACGG CCTGATGAAA
    GCCAGGAATG ACTGGATGGC AGGTCCGGGT AGGCACGTAA ACCAAGGAAC CAAATTCAGT
    GTCATAGCCA CATTTGCGAT TCTCCAGAGT TTGGCGCATT CCATCAAACT ACTCAACTTC
    CATGGAATCA AACCGTTCTA CAATAATCTG GCCGAATTCC GCACCACCGA GGAGGAGAAA
    GGAGGAAAAG GGTCAAAGCT CAAGCGGCAA GTCTTGGAGG ATGAGAACTT TCAGAAGATG
    ATGGACATGA TTGAGGGTTG GATGAAGATT GATGGGTTCC TCGGACACCC CAAGCTGGAA
    TATCTTTGTG AGACCCTGGT CAACCACTTC ATGGATGCTG GCGAGGGCTC CAATACCAGA
    GCCATTGTAT TCAGTGAATA CCGAGACAGT GCCGAAGAGA TTGTGCGCAT TCTCAACAAG
    CAACCCCTTA TCAAGGCCAC AGTCTTCGTT GGACAAGCGG ACTCGAAGAG GAGTGAGGGC
    ATGAAACAGA AACAACAAAT TGAGACGATC GAGAAGTTCA AGAACGGCGC TCACAACGTT
    CTGGTCGCAA CATCCATTGG CGAGGAGGGT CTTGATATTG GCCAAGTGGA TCTCATTGTC
    TGCTACGATG CATCAGCGTC ACCGATCCGA ATGCTTCAGC GTATGGGTCG TACCGGCCGC
    AAGAGAGCGG GTAACATTGT ACTTTTGTTG ATGAAAGGGA AAGAGGAAGA CAAATTCAAC
    GAGGCGAAGG ACAACTACGC GACCATGCAG AGAATGATAT GCGAGGGCAG CCGCTTTACC
    TTTCGTCACG ACCTTTCTTC ACGTATTGTG CCACGAGACA TACGACCTGA GGTGGAAAAG
    AAGGTTGTGG AGATTCCACT TGAGAACTCC CAAAATCCAG AGCTCCCAGA ACCAAAAAGA
    TCTGCCGCAA GAATGAGGAC AAAGCCGGCA AAGAAGAAGT TCAACATGCC TGACGGTGTT
    GAAACAGGGT TCATCAAGGC TTCTTTCTTT GGTCAAGCTG GAGCTAAGAC AGCCAAACCA
    CCCGCTAGGC CACCTGCGCC TAAGGAAACT GACTTTATTG CCGAACGGCC CAAACTCGAA
    AGCATTTTGC TGAGCACGTC GCAGGAAAAC GAGCTCCGCA GAAACTATAC AAAGATACCC
    CTCGGACACT CGAAGGTTGA AGAGTTGGAT ATAGACTGGT ATCGCCATCC AACATCCAGA
    CGAGTGGTCC AGAAAACCAT CCATGTCAAA CACGGGGAAT ATACCAAGCG ATGTGTGAAA
    CTGTTTAGGA GCCTGGCAAA GTCTCAAGCT CCTGCGAATA GGTATACTAA GCCATACGGT
    GAAACAGACA CGTCCTCCTG GGAATTGATA CCATTGCCGC CCCTTGCGGA CGAAACAGAA
    GGAGAAACGT CACGAAAGGG GCAAAAGAAG AGGCCGCGCC TCGAGAGTGG GCAGGAAGCA
    GAGGAAGCAG AGCAATATGC CGCACCTAAG AAACGTCAAG CGACAGCAAA AACCAAATCT
    ACCGGGGTCT CCAAACAGAC CAACAAGCCT CGAGCACGAC ATACCGCACT TATCAGCGAC
    TGTGAAGAGG GTGGCAACGA ATATGATGGC AATGTCGACG ATGACGAACA AAGTAGGCCC
    CGAAACTTCC GCAGCAAGGG TCGTGGACGT GGCAGCGGGC GGGGAAAAAA ATCTCAACCT
    AAACAAGGAG ACCCCAACGT GGACTACGGT GACGACTGCA CCCGCACCAG CGACATGGAA
    ATGGGTACCG ATGGCTCTGA TGACGGTGCC GACCTCGAGG ACTTTATCGT CAGCGATGGC
    GAAGTCACCT CGAGTTTACA GCATCGTCCC CGAGGGTCCA CCTCGCCGAC AACAGCTCCT
    GACGCAGGGT CGTCTTCCTT GTCCTCGAAA ACGGGACGCA AGCAGCAAGC ACCAGATTCC
    TTCGCGTCGG ATGAAGATGA TGGCGATGTG TTTGGACCCA AATTTGTACC GGTAACCGCG
    TCGGCAGCTA AAGGCTCGTT GCCATCAACA GCGAGACGAG AGAAGCCCAA GCCGTTTTAT
    GTGCCGGTAG AATTACCTGC AACCCAGGAC ACAACGGACG GAGACGACGA CTTACCCGAT
    ATCGAATTTC TGTCTGCTAA GAGAAAGAGG GAAGGCACAG GTACGGGGAT GCGAACAGGG
    AGCCCAGGAC ATGTTAAAGT TGGCGACACC TCGAAAGGGG GCAGCGGCGG AGATCAAACT
    CGAGAGAAAC CTTCTGGTGG TGCGGCGTCC CATGCCCGAG CGAGGAAGCG AACTGTGGTG
    ATGGATAGTG ATGATGACCA GGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B