pks-4 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	pks-4
Entrez Gene ID	3879386
Full Name	polyketide synthase-4
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011396978.1	XP_011395280.1	polyketide synthase 4, variant
XM_011396977.1	XP_011395279.1	polyketide synthase 4

Pathways from BioSystems

Homologies

Neurospora crassa	NCU08399	XP_963238.1
Magnaporthe oryzae (rice blast fungus)	MGG_11638	XP_003718917.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pks-4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pks-4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf01857	XM_011396977.1 Latest version!	Neurospora crassa OR74A polyketide synthase 4 (pks-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ONf01857	XM_011396978.1 Latest version!	Neurospora crassa OR74A polyketide synthase 4, variant (pks-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pks-4 ( XM_011396978.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011395280.1 Neurospora crassa OR74A polyketide synthase 4, variant (pks-4), mRNA.

Price & Availability↑

CloneID	ONf01857
Clone ID Related Accession (Same CDS sequence)	XM_011396978.1 , XM_011396977.1
Accession Version	XM_011396978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	polyketide synthase 4, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507).

ATGTCTTACA AGATTCCGCT GTCTGAGCCC ATCGCCATTG TGGGCAGCAG CTGCCGCTTT 
ACGGGCGAAG CCACATCCCC CGCCAAGCTG TGGGAGCTTC TGAAGGACCC CAAGGATCTT 
ACGCGCGAAG TCCCCAAGGA TCGGTTTAAC GTCGAGGGCT TCTATCACCC CGATGGTGAG 
TATCACGGCA CTACCAATTC GGCCAAGGCC TACTTCCTCG AGCAAGACCA TCGCCTCTTC 
GATGCCAGCT TCTTCAACAT CACCCCGAAG GAGGCTGAGG CCATTGATCC CCAACAGCGT 
ATGCTGCTGG AGGTAGTGTA CGAGGCTCTC GAGTCCGCCG GTTACACCCT CCAAGATTAC 
TCCGGCAAGA AGGTTGCCGT CTTCGCTGGT GTCATGACAG CCGATTACGA CACCCTTTCT 
CAGCGCGACG ATCTGTCTGT CAGCCAGTAC TACGCCACTG GCAATGCTCG CTCCATCATT 
TCGAACCGCG TGTCGTACTT CTTCAACTTC CATGGTCCTT CTATGACCAT CGACACAGCC 
TGCTCTTCCA GTCTGGTCGC CCTTCACCAA GCCGTTCTCT CTCTTCGCTC GGGTGAGGCT 
GAGATGGCAT GCGTTTCAGG TGTCAACTTG ATTCTCACCC CCGAGCAGTT CGTCGTCGAG 
TCGAGCCTGC ACATGCTTAG TCCCACTGGC CGCTGCCACA TGTGGGACGA TCGCGCCGAC 
GGTTATGCAC GTGGCGAGGG TGTCGCCGCG ATCTTCATCA AGCCTCTTAG CAAGGCTTTG 
GCCGACGGTG ATCGTATTGA GGCCATCATC CGCGAGACCG GTGTCAACTC TGACGGCCGC 
AGCAAGGGCA TCACCATGCC CAACTGGGAG GCACAGTCCG CCCTGATCCA AGACACCTAT 
CGTCGCTCTG GCTTGAACGC CAGAGACCCC ATTGATCGTT GCCAATTCTT CGAGGCTCAT 
GGTACCGGCA CTGCTGCTGG TGATCCCAAT GAGGCTAGAG CGATTGAGGA TGCCTTTTTC 
GGCCGTAACG TCAGCACTTC GAGCCAAGAC ACGGCCGAAG TGTCAAAGCT ACTTGTGGGC 
TCTGTCAAGA CTGTCATCGG TCACACCGAA GGTGCCGCCG GATTGGCCGG TCTCTTCAAG 
GTGGTTCATG CCATGATCAA CGGCACTGTG CCTCCTAATT TGCACTTCAA CAGACTTCAG 
CCAGCTGTCG CCAAGTATTA CTCGCACCTT CATGTGCCCA CCAAGGCCCT TGCCTGGCCT 
TCTGTCGCGG CTGGACAGCC CAGACGTGCC ACTGTTAATT CCTTCGGATT CGGAGGAACC 
AACTCGACTG CAATTTGCGA AATGTACGTT CCGGAGATTC ATGACCCGGT CGCCAAGATC 
TTTGCTCCGA CCCTCATTGT GCCCAAGCTC ATCAATTCGG GCTCTCTTTC GGCTGATGGC 
CAGACTCGTC TGCCCCTGGT CCTTTCCGCC AGCTCGCCCA GGTCTATGGG CGCTGTTGCC 
AAGTGCTATC GCGATTACCT CGCGCAGGTG TCGTTGCCTC TTGAGGAGGC TGCGTGGTAT 
GCTTACAAGA ACCGCACCGC ATTTCCTTAC CGCACTGCCG TTAGCGGATC TTCACTGTCG 
GACATTATGA AGGGTCTCGA CACCTTGATT GCAAAGGCCG AGGCTGGCAA AGCAGCTACA 
ATCAGTGTCC GCGCTCGCCC CGAGACCGAT GTTCCCAAGA TCCTGGGTAT CTTTACCGGC 
CAAGGTGCTC AGTGGGCCAC CATGTCCCGT GGTTTACTGC AGAGCAACAA TGTTTATGCT 
CAGTCCATTC GCAACCTCGA CCAGGTCCTT CAGGCCTGTC CTCATCCGCC CTCGTGGTTC 
CTTGAACAGG AGATCATGGC CGACGCAAGT CTCTCTAGAG TTGGGAAGGC GGCCATCTCC 
CAGCCTCTCT GCACCGCGGT CCAGATCGCC CTCATCGATC TTCTCGCTCA CTTGGGCATT 
ACTTTCCACA CCGTTGTCGG TCATTCATCC GGCGAAATTG CTGCGGCTTA CGCGGCGGGC 
AAACTTTCTG CTCGCGATTC GATTCTCATC TCGTATTATC GCGGAAAATA CGCCCACCTC 
GCCAGCGGTG CCCAGGGCAG TCTGGGTGGC ATGATGGCTG CAGGTTTGAC GAAGGCAGAA 
GCCGAAGAGC TTTGCGCCCT GCCTCAGTTT GGCAACCGCG TTTGCGTTGC TGCCAGCAAC 
GGACCCAGCA GTGTCACCTT GTCAGGCGAC CTTGACATTG TGCAAAAGGT CCATGATCAC 
CTTGTTGACG AGAAGAAGTT TGCGAGGATG CTGCAGGTCG ACACAGCCTA TCACTCGCCT 
CACATGGTGC GCCCTGCTAA TGAGTACATT GAGGCCCTCA AGGCTTGCAA CATTGCTCCA 
TTGCAGGATG GCAACGGCAC CACCTGGGTG TCCAGCGTCT ACGGTCATGG TGAACCCACG 
TCTGAAGAGC TCGCGTCCTC CTACTGGCGC GACAACATGG TCAATGCAGT CCTTTTCTTT 
GAGGCTGTCA GCACTGCTCT GGAAGGACAA GGTCCCTTTG ACTGTGCCAT CGAGGTTGGA 
CCTCACGCGG CCCTGCGCGG CCCCGTCATT CAGACCATGA AGGCCAAGTC GGGAGAGGCC 
ATTATCTACT CTGGTCTTCT CGACAGAAAG CTCGACGATC GCATTGCTTT TGCGGACTTC 
TTGGGCTGGA TGTGGTCTAA CTTCGGCTCA TCGTCATCAT TGATTGAGAG GTTTGTTCAG 
AGCTCAGTCA ATCCTGAATT GGCTCACCTT CGTTTCCAGC AGGGTCCCAA TTACCCTTGG 
GACCACTCGC ATACCTACTA CCGCGAGTCA CGCATTTCTC GTCAGTATCA CTTCAGGACC 
CACAAACCTC ATGAGCTTCT CGGTATTCGT ACCAGGGATG ACAACGAGCA TGAGCTCAGA 
TGGCGCAACA TCATGAAGTA CGCCAAGCTT CCATGGGCCC AGGGCCACAA GTTCCAAGGA 
CAGGCGCTGC TTCCGGCTTC AGCATACTGT ATCATGGTGT TGGATGCTGC ACGCGTCCTT 
TCGGGTAACC GCGCCGCTTC TGTTGTCGAG CTGCGCGACT TGAAGTTTAT GGCTGGCATC 
ACGCTGGAGC CTGAGTCTCC CGGTGTTGAG ACCCTCTTCT CTTTGGTGGT GTCCAACTCG 
CCCAAGAACG CCTCCACTAT TGAGGCCTTA TTCACGCTGA CCTCGGCGCC TGCTGATGGC 
ACCATTGACA TGAAGCTGAA TTTCAGTGGA CAGCTTCATA TTGTCCTCGG AGAGAAGTCT 
CCCAACGCAC TTAGCCGTCG TCCCGCGCAG CGCGCTGAGA CCCTCACCGC CCAACCCGAG 
GCGTTCTACA AGATGATGGC AGGCACAGGT CTGGATTATA ACGGACCTTT CAAGGGATTG 
ACGGCCATTG AGCGTCGCTA CCAGTTTTCC TCTGGCACTG TGAAGAGGTT CCATGCAGAT 
GACCAGACTG GCTTGGACAT CAGCCCTGCC ACGCTGGACA GCTGCTTGCA GACTGCGTTC 
CTCACTGTTT CTTCCCCTGG TGACGGGTCC CTGTGGACTT CGTTCCTTCC CAAGGTGATG 
AGTCGTGTCA AGTTCAACCT TGCTACCTGT CGCAGCGGTG GGCGCGATGA TAACCTTGTT 
GTTGATGCCT ATCTTACTAA AGGCACTCCT TTCTCCAAAA CGGCTGCAGC CTCTTTCACA 
GCTGAGATCA ACATCTTCAA CCCCGAGGGC AACCTGGAGA TTCAAATTGA GGGACTGACC 
GTTGGCTCCT TCACATCGAC TCGCCCTGAA AATGACAAGG AGCTTTATCT CACTACCGTC 
TACGAACTCG ACCCTGAGGA TGAGATCGTT GCTTCTCCAG CGGAGGCCCT CAATGGCGTG 
AGTCCCATGC TCTTCGAGAG CTGTGCCAGA GTTGCCTCGT TTTACGTCGA GCGTGCCGCG 
CTTAAACGCC TCAAGGAAAC GCCCAGGGAT CTGCTTGAGC CAGCGGGTTA CCAGACCGAG 
ACTCGCTTGG TGTCCAACTC CTGGCCCAAC GAGACCGAGC AGAGTCTGGA GAAGTTCATC 
CGTTCTTCGC CCTATTTCAT GACTCTCGAT TTCGTTCGCC ACCTGGGAGA GAATCTCCCT 
GACGTCCTTC CGGGCATGCT CCCTACCTTG GTTCAAGAGG CACAGCAGCT CGCCTATTTC 
CAGAAGCAGG TCGGCCGTGT TGTTCGCCAG ATCGTTCACA AGTACCCTCG CATGAACATC 
CTTGGCCTGA CTGATCCCGA ATTGGGCTTG ACCGAGCATC TCATGGCAGC ACTCGATGGA 
TCGTTCCTTA GCTACCGCAT TGGTGGCAAG CCTGAGACAA ACCTCAAGGA GCGTTCTCCT 
CTCGCCGAAG GGATTCGCAA GAAGATCATG ATTGACGAGA TTGACTTCGA GGGCGATGCT 
GAATCTGTCG GAGGACCTTA TGATCTTGTG GTTCTCAACA CGTCCATCCT TGAGGACAAG 
AAGACTCATT CCGCGTTACA GCTTATCAGG GATTCGATGC GTCCTGGCGG CTTCCTTGTT 
GTCGTCCATG CATCTCGTAC TCCACTCAAG GAGAGAATGC TTCGTTGCGC TGGCCGTACC 
TCGAGCAACG ACGGAATCGC CACACCTCCT GACTGGCCTG ATCTCATCGA GGAATGCGGA 
TTTGAGAACA CAGCTAAGAA CTCGGATATG TGGTTCCATC CTGGATTCAC CCTCAGTGTA 
AGACAGGCAA TGTCACAGAG AAAGAAGCAG CTGCTGTGGC CTCTGCATCA CGCCACCGGA 
ACTCCTCTCG TGAAGCATCT TTTGATTGTG GGTGGTAAGA AGGTCTGGAC GTCTTGCATT 
TCCTCTGGTG TCTTCCAGGA ACTTGCCTCT TTCTGTGGAA AGATCACCGC TGTTGACTCC 
CTGGAGGATA TCCACAATGA TGAATTGCTC TCATCTTGCA CTGCAGCCAT CATCCTTGGC 
GATATTGATC AGCCTATTCT TGCTGATATG ACGGCCGAGC GAATGGACAC CATTCGGTCC 
TTGCTGCGCC CCCAAATGCT CATTATGTGG GTTACTCATA ATGCCAGGTT CGACAACCCC 
GATCATGCCG CATCGCTTGG CTTCACTCGA ACGGTTACCG GTGAAGTCCC TGGCTTGCAT 
ATACAAGTGC TCGATCTCGA CACCATTGAC ACCTGTCCGG CTGTTCGCGC CGTGTCGAAG 
ACGTTCGCTC GTTTCATCGT TCATTTCCTC GGCCCCAAGA ATGAGGATGA TGAGCCCCTG 
TGGGTTCGTG AGCACGAAGT GCACGTTGAG GGTGGCCGCT TGATCGTTCC TCGCGTTCTC 
CCGTGGAAAG AGGGTAATCA GCGTGTCAAT GCGCCCCGCC GCGTGATGAC CGACACAGTC 
AACACTCTGG AAGACGTTGT CGAGGTCCTC GCTTCAGCCT CTGGCCGGTA CGAGACTCGG 
GTCGAGAAGA GCGAAGCCTA TCCTTCGCAT GTTGAATCGG CGATGCACGT GGACTACAGC 
ACTGTCAAGG TGTTCAACAT CGGTGCCATT TGCTCCGGTC ATATCTGCAT TGGCCGCAAC 
AACGAGACTG GCAAGGCTCA AGTTGCCCTC TCTAAATCGA ATAAGTCCTT TATTTCGACG 
GCCTCTACAA TCTTCAGCAC GCTCCCTGAA GGCACTGTCG GCGTCTGTGA GCCGCTCTAC 
CTTAGCTTGC TCAGCCGGTA TATTTACGCT CTCACTATCG TCAACATGGC TAATGGCAGG 
TCTGTCATGA TCATTGAGCC TGATGGTCTC CTCTTCCAAT GCGCCAAGGA AGTTTGTAGC 
AACCGCGGCA TCTGGTTTAT GGGTGCTTCC ACGGATGAGC GTCGTAGCCG AGTTGTTCCC 
GATATTGCCT TTGTTCATTC ACAGTCAACT TCCAGGCAGA TCAAGGCCCT CTATCCCCTC 
GACGGGGCTT GCATTGTCAA CATGCTGCCC AAGACTCACG ACGTCTCTCG CATGCTTGTT 
GACTCGGTGC CTGAGAATTG CGAGTATCTC ACTCAGGATA AACTTATCTC GACTCACTTC 
ACGGCGCACC CCAACGCGGC ATTCGTTCAG AAGCTATGGG AAGAGGCTGT GACACTTTCT 
TACAACAGCA TGAAGAGCAT GCTGCTTAAT GCCATCGAGC CTTCTTTCGA CATCCTGGAC 
CCCATCACTT TGCCCAAGTT GCTTGACTCT GCTGTGTCTT CTGAGGCTTT CCAGATGCTC 
GACTGGAAGG CCGAGAGGTC TATCGCTCAC ATGATCAAGC CTATCGTGGG TGAGAAGATG 
CTTCGCCCTA ACAGTACCTA TGTCCTGGTT GGCATCACTC GCGACATGGG CCAGAGTCTC 
TGCTCTCTCT TCATCGAGCA GGGTGCTCGC CATCTTGTCT TGTGCAGTCG AAACCCCCCG 
AAGGAGCAGC CTCAGTGGCA GAAGGAGCTC AATGCTCGCG GCATCAAGGT CAAGTTCGAG 
GCGATGGATG TTACCAACCT CAACACGGTG ATTGCCTTCA AGACGAAGCT TGCCGAAGAT 
TCTTGGCCGT CTGTCGGGGG CGTGGTCAAC GGTGCCATGG TTCTTGACGA CCGTGTGTTC 
TCCGAGATGT CCGTTGAGAC TTGGAATCGC GTCATGAATC CAAAGGTGGT CGGTTCGAGG 
AATCTCGACA TAGTCTTCAA CTCTCCGGAT ATGGATTTCT TTATCATGAC CTCGTCTTTC 
GCTGCCATTG GCGGCCATGC CGGTCAATCG AACTATGCCG CGGCTAACAT GTACATGAAC 
GGCATTGCCG CTTCTCGTCG TTGCCGCGGA CTGCCGGCCA CGGCTCTGAA CATTGGGGTC 
GTCTACGGCC TTGGCTTCTT GCACCGTGAG AAGGACGATC TGTACGCCGG TCTCGAGCGT 
GAGGGATACC CGCCCATTTC CGAGCGTGAT CTTCATCACA TGTTCCTCGA GGCCATTGCT 
GCTGGAAAGC CCGTGGGACA CACCGTCTAC GACATCACTA CGGGCATGAA CCGATTCCGC 
ATGGACGCAC CGAATCAGCA CTGGCAGCTC GATCCTCGTT TCTCACACAA TACCCTCCCC 
GAGGAGGACG AGACCGCCGC TGGTTCTGCC GGCCAGCAGA TCAAGTCTCT TGCCGAGGAT 
CTTGATGCTG CCACCACCAA AGAGGACGTG ATGAAGGTGT ACGTGCCTGC CTTTATTGGC 
CGCTTGGAGG CACTTCTGCA GATTGCCGAG GGCGAAATCA CACCTGCCAA CAGCGTCACT 
GAGCTTGGTG TCGACTCTCT TATCGCAGTC GAAATCCGCT CCTGGATTTG GAAGACGGTC 
GCCCAAGATG TTGCGGTGAT GAAGATCCTT AGCGCTACCA GCATTGCCCA GTTGTGCGAG 
GGAATTGCCA GTGATCTTTT GGTCGCCCGC GGTGCTGCAG CCAAGGATTC TACTGCTCCT 
ACTGAACAGA AGGAGACTCC CGCTGGCTCT CAGACTTTGC AGGCTGTCAG TGGCTCGGTC 
AAATCAGCTC CGAGCACCAC TCCATCTCTC GAGGCTTCGG CTAGCACTTT GACTGAAGCC 
ACTGTGGTTT CGATTGTCAG CACTTCCACT GTGGTATCCA GCGCCAGCAC TGCTACCAGC 
GTTGATGGTG ACGCTGAGGA GGATAACGCC TTGTCGGCTA GTTGGGAGGA CGTAGGAAAG 
AAGGAGTCTT CCATTTTTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011395280.1
CDS	2978..10681
Translation	MSYKIPLSEPIAIVGSSCRFTGEATSPAKLWELLKDPKDLTREVPKDRFNVEGFYHPDGEYHGTTNSAKAYFLEQDHRLFDASFFNITPKEAEAIDPQQRMLLEVVYEALESAGYTLQDYSGKKVAVFAGVMTADYDTLSQRDDLSVSQYYATGNARSIISNRVSYFFNFHGPSMTIDTACSSSLVALHQAVLSLRSGEAEMACVSGVNLILTPEQFVVESSLHMLSPTGRCHMWDDRADGYARGEGVAAIFIKPLSKALADGDRIEAIIRETGVNSDGRSKGITMPNWEAQSALIQDTYRRSGLNARDPIDRCQFFEAHGTGTAAGDPNEARAIEDAFFGRNVSTSSQDTAEVSKLLVGSVKTVIGHTEGAAGLAGLFKVVHAMINGTVPPNLHFNRLQPAVAKYYSHLHVPTKALAWPSVAAGQPRRATVNSFGFGGTNSTAICEMYVPEIHDPVAKIFAPTLIVPKLINSGSLSADGQTRLPLVLSASSPRSMGAVAKCYRDYLAQVSLPLEEAAWYAYKNRTAFPYRTAVSGSSLSDIMKGLDTLIAKAEAGKAATISVRARPETDVPKILGIFTGQGAQWATMSRGLLQSNNVYAQSIRNLDQVLQACPHPPSWFLEQEIMADASLSRVGKAAISQPLCTAVQIALIDLLAHLGITFHTVVGHSSGEIAAAYAAGKLSARDSILISYYRGKYAHLASGAQGSLGGMMAAGLTKAEAEELCALPQFGNRVCVAASNGPSSVTLSGDLDIVQKVHDHLVDEKKFARMLQVDTAYHSPHMVRPANEYIEALKACNIAPLQDGNGTTWVSSVYGHGEPTSEELASSYWRDNMVNAVLFFEAVSTALEGQGPFDCAIEVGPHAALRGPVIQTMKAKSGEAIIYSGLLDRKLDDRIAFADFLGWMWSNFGSSSSLIERFVQSSVNPELAHLRFQQGPNYPWDHSHTYYRESRISRQYHFRTHKPHELLGIRTRDDNEHELRWRNIMKYAKLPWAQGHKFQGQALLPASAYCIMVLDAARVLSGNRAASVVELRDLKFMAGITLEPESPGVETLFSLVVSNSPKNASTIEALFTLTSAPADGTIDMKLNFSGQLHIVLGEKSPNALSRRPAQRAETLTAQPEAFYKMMAGTGLDYNGPFKGLTAIERRYQFSSGTVKRFHADDQTGLDISPATLDSCLQTAFLTVSSPGDGSLWTSFLPKVMSRVKFNLATCRSGGRDDNLVVDAYLTKGTPFSKTAAASFTAEINIFNPEGNLEIQIEGLTVGSFTSTRPENDKELYLTTVYELDPEDEIVASPAEALNGVSPMLFESCARVASFYVERAALKRLKETPRDLLEPAGYQTETRLVSNSWPNETEQSLEKFIRSSPYFMTLDFVRHLGENLPDVLPGMLPTLVQEAQQLAYFQKQVGRVVRQIVHKYPRMNILGLTDPELGLTEHLMAALDGSFLSYRIGGKPETNLKERSPLAEGIRKKIMIDEIDFEGDAESVGGPYDLVVLNTSILEDKKTHSALQLIRDSMRPGGFLVVVHASRTPLKERMLRCAGRTSSNDGIATPPDWPDLIEECGFENTAKNSDMWFHPGFTLSVRQAMSQRKKQLLWPLHHATGTPLVKHLLIVGGKKVWTSCISSGVFQELASFCGKITAVDSLEDIHNDELLSSCTAAIILGDIDQPILADMTAERMDTIRSLLRPQMLIMWVTHNARFDNPDHAASLGFTRTVTGEVPGLHIQVLDLDTIDTCPAVRAVSKTFARFIVHFLGPKNEDDEPLWVREHEVHVEGGRLIVPRVLPWKEGNQRVNAPRRVMTDTVNTLEDVVEVLASASGRYETRVEKSEAYPSHVESAMHVDYSTVKVFNIGAICSGHICIGRNNETGKAQVALSKSNKSFISTASTIFSTLPEGTVGVCEPLYLSLLSRYIYALTIVNMANGRSVMIIEPDGLLFQCAKEVCSNRGIWFMGASTDERRSRVVPDIAFVHSQSTSRQIKALYPLDGACIVNMLPKTHDVSRMLVDSVPENCEYLTQDKLISTHFTAHPNAAFVQKLWEEAVTLSYNSMKSMLLNAIEPSFDILDPITLPKLLDSAVSSEAFQMLDWKAERSIAHMIKPIVGEKMLRPNSTYVLVGITRDMGQSLCSLFIEQGARHLVLCSRNPPKEQPQWQKELNARGIKVKFEAMDVTNLNTVIAFKTKLAEDSWPSVGGVVNGAMVLDDRVFSEMSVETWNRVMNPKVVGSRNLDIVFNSPDMDFFIMTSSFAAIGGHAGQSNYAAANMYMNGIAASRRCRGLPATALNIGVVYGLGFLHREKDDLYAGLEREGYPPISERDLHHMFLEAIAAGKPVGHTVYDITTGMNRFRMDAPNQHWQLDPRFSHNTLPEEDETAAGSAGQQIKSLAEDLDAATTKEDVMKVYVPAFIGRLEALLQIAEGEITPANSVTELGVDSLIAVEIRSWIWKTVAQDVAVMKILSATSIAQLCEGIASDLLVARGAAAKDSTAPTEQKETPAGSQTLQAVSGSVKSAPSTTPSLEASASTLTEATVVSIVSTSTVVSSASTATSVDGDAEEDNALSASWEDVGKKESSIFL

Target ORF information:

RefSeq Version	XM_011396978.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A polyketide synthase 4, variant (pks-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396978.1

ORF Insert Sequence:

ATGTCTTACA AGATTCCGCT GTCTGAGCCC ATCGCCATTG TGGGCAGCAG CTGCCGCTTT 
ACGGGCGAAG CCACATCCCC CGCCAAGCTG TGGGAGCTTC TGAAGGACCC CAAGGATCTT 
ACGCGCGAAG TCCCCAAGGA TCGGTTTAAC GTCGAGGGCT TCTATCACCC CGATGGTGAG 
TATCACGGCA CTACCAATTC GGCCAAGGCC TACTTCCTCG AGCAAGACCA TCGCCTCTTC 
GATGCCAGCT TCTTCAACAT CACCCCGAAG GAGGCTGAGG CCATTGATCC CCAACAGCGT 
ATGCTGCTGG AGGTAGTGTA CGAGGCTCTC GAGTCCGCCG GTTACACCCT CCAAGATTAC 
TCCGGCAAGA AGGTTGCCGT CTTCGCTGGT GTCATGACAG CCGATTACGA CACCCTTTCT 
CAGCGCGACG ATCTGTCTGT CAGCCAGTAC TACGCCACTG GCAATGCTCG CTCCATCATT 
TCGAACCGCG TGTCGTACTT CTTCAACTTC CATGGTCCTT CTATGACCAT CGACACAGCC 
TGCTCTTCCA GTCTGGTCGC CCTTCACCAA GCCGTTCTCT CTCTTCGCTC GGGTGAGGCT 
GAGATGGCAT GCGTTTCAGG TGTCAACTTG ATTCTCACCC CCGAGCAGTT CGTCGTCGAG 
TCGAGCCTGC ACATGCTTAG TCCCACTGGC CGCTGCCACA TGTGGGACGA TCGCGCCGAC 
GGTTATGCAC GTGGCGAGGG TGTCGCCGCG ATCTTCATCA AGCCTCTTAG CAAGGCTTTG 
GCCGACGGTG ATCGTATTGA GGCCATCATC CGCGAGACCG GTGTCAACTC TGACGGCCGC 
AGCAAGGGCA TCACCATGCC CAACTGGGAG GCACAGTCCG CCCTGATCCA AGACACCTAT 
CGTCGCTCTG GCTTGAACGC CAGAGACCCC ATTGATCGTT GCCAATTCTT CGAGGCTCAT 
GGTACCGGCA CTGCTGCTGG TGATCCCAAT GAGGCTAGAG CGATTGAGGA TGCCTTTTTC 
GGCCGTAACG TCAGCACTTC GAGCCAAGAC ACGGCCGAAG TGTCAAAGCT ACTTGTGGGC 
TCTGTCAAGA CTGTCATCGG TCACACCGAA GGTGCCGCCG GATTGGCCGG TCTCTTCAAG 
GTGGTTCATG CCATGATCAA CGGCACTGTG CCTCCTAATT TGCACTTCAA CAGACTTCAG 
CCAGCTGTCG CCAAGTATTA CTCGCACCTT CATGTGCCCA CCAAGGCCCT TGCCTGGCCT 
TCTGTCGCGG CTGGACAGCC CAGACGTGCC ACTGTTAATT CCTTCGGATT CGGAGGAACC 
AACTCGACTG CAATTTGCGA AATGTACGTT CCGGAGATTC ATGACCCGGT CGCCAAGATC 
TTTGCTCCGA CCCTCATTGT GCCCAAGCTC ATCAATTCGG GCTCTCTTTC GGCTGATGGC 
CAGACTCGTC TGCCCCTGGT CCTTTCCGCC AGCTCGCCCA GGTCTATGGG CGCTGTTGCC 
AAGTGCTATC GCGATTACCT CGCGCAGGTG TCGTTGCCTC TTGAGGAGGC TGCGTGGTAT 
GCTTACAAGA ACCGCACCGC ATTTCCTTAC CGCACTGCCG TTAGCGGATC TTCACTGTCG 
GACATTATGA AGGGTCTCGA CACCTTGATT GCAAAGGCCG AGGCTGGCAA AGCAGCTACA 
ATCAGTGTCC GCGCTCGCCC CGAGACCGAT GTTCCCAAGA TCCTGGGTAT CTTTACCGGC 
CAAGGTGCTC AGTGGGCCAC CATGTCCCGT GGTTTACTGC AGAGCAACAA TGTTTATGCT 
CAGTCCATTC GCAACCTCGA CCAGGTCCTT CAGGCCTGTC CTCATCCGCC CTCGTGGTTC 
CTTGAACAGG AGATCATGGC CGACGCAAGT CTCTCTAGAG TTGGGAAGGC GGCCATCTCC 
CAGCCTCTCT GCACCGCGGT CCAGATCGCC CTCATCGATC TTCTCGCTCA CTTGGGCATT 
ACTTTCCACA CCGTTGTCGG TCATTCATCC GGCGAAATTG CTGCGGCTTA CGCGGCGGGC 
AAACTTTCTG CTCGCGATTC GATTCTCATC TCGTATTATC GCGGAAAATA CGCCCACCTC 
GCCAGCGGTG CCCAGGGCAG TCTGGGTGGC ATGATGGCTG CAGGTTTGAC GAAGGCAGAA 
GCCGAAGAGC TTTGCGCCCT GCCTCAGTTT GGCAACCGCG TTTGCGTTGC TGCCAGCAAC 
GGACCCAGCA GTGTCACCTT GTCAGGCGAC CTTGACATTG TGCAAAAGGT CCATGATCAC 
CTTGTTGACG AGAAGAAGTT TGCGAGGATG CTGCAGGTCG ACACAGCCTA TCACTCGCCT 
CACATGGTGC GCCCTGCTAA TGAGTACATT GAGGCCCTCA AGGCTTGCAA CATTGCTCCA 
TTGCAGGATG GCAACGGCAC CACCTGGGTG TCCAGCGTCT ACGGTCATGG TGAACCCACG 
TCTGAAGAGC TCGCGTCCTC CTACTGGCGC GACAACATGG TCAATGCAGT CCTTTTCTTT 
GAGGCTGTCA GCACTGCTCT GGAAGGACAA GGTCCCTTTG ACTGTGCCAT CGAGGTTGGA 
CCTCACGCGG CCCTGCGCGG CCCCGTCATT CAGACCATGA AGGCCAAGTC GGGAGAGGCC 
ATTATCTACT CTGGTCTTCT CGACAGAAAG CTCGACGATC GCATTGCTTT TGCGGACTTC 
TTGGGCTGGA TGTGGTCTAA CTTCGGCTCA TCGTCATCAT TGATTGAGAG GTTTGTTCAG 
AGCTCAGTCA ATCCTGAATT GGCTCACCTT CGTTTCCAGC AGGGTCCCAA TTACCCTTGG 
GACCACTCGC ATACCTACTA CCGCGAGTCA CGCATTTCTC GTCAGTATCA CTTCAGGACC 
CACAAACCTC ATGAGCTTCT CGGTATTCGT ACCAGGGATG ACAACGAGCA TGAGCTCAGA 
TGGCGCAACA TCATGAAGTA CGCCAAGCTT CCATGGGCCC AGGGCCACAA GTTCCAAGGA 
CAGGCGCTGC TTCCGGCTTC AGCATACTGT ATCATGGTGT TGGATGCTGC ACGCGTCCTT 
TCGGGTAACC GCGCCGCTTC TGTTGTCGAG CTGCGCGACT TGAAGTTTAT GGCTGGCATC 
ACGCTGGAGC CTGAGTCTCC CGGTGTTGAG ACCCTCTTCT CTTTGGTGGT GTCCAACTCG 
CCCAAGAACG CCTCCACTAT TGAGGCCTTA TTCACGCTGA CCTCGGCGCC TGCTGATGGC 
ACCATTGACA TGAAGCTGAA TTTCAGTGGA CAGCTTCATA TTGTCCTCGG AGAGAAGTCT 
CCCAACGCAC TTAGCCGTCG TCCCGCGCAG CGCGCTGAGA CCCTCACCGC CCAACCCGAG 
GCGTTCTACA AGATGATGGC AGGCACAGGT CTGGATTATA ACGGACCTTT CAAGGGATTG 
ACGGCCATTG AGCGTCGCTA CCAGTTTTCC TCTGGCACTG TGAAGAGGTT CCATGCAGAT 
GACCAGACTG GCTTGGACAT CAGCCCTGCC ACGCTGGACA GCTGCTTGCA GACTGCGTTC 
CTCACTGTTT CTTCCCCTGG TGACGGGTCC CTGTGGACTT CGTTCCTTCC CAAGGTGATG 
AGTCGTGTCA AGTTCAACCT TGCTACCTGT CGCAGCGGTG GGCGCGATGA TAACCTTGTT 
GTTGATGCCT ATCTTACTAA AGGCACTCCT TTCTCCAAAA CGGCTGCAGC CTCTTTCACA 
GCTGAGATCA ACATCTTCAA CCCCGAGGGC AACCTGGAGA TTCAAATTGA GGGACTGACC 
GTTGGCTCCT TCACATCGAC TCGCCCTGAA AATGACAAGG AGCTTTATCT CACTACCGTC 
TACGAACTCG ACCCTGAGGA TGAGATCGTT GCTTCTCCAG CGGAGGCCCT CAATGGCGTG 
AGTCCCATGC TCTTCGAGAG CTGTGCCAGA GTTGCCTCGT TTTACGTCGA GCGTGCCGCG 
CTTAAACGCC TCAAGGAAAC GCCCAGGGAT CTGCTTGAGC CAGCGGGTTA CCAGACCGAG 
ACTCGCTTGG TGTCCAACTC CTGGCCCAAC GAGACCGAGC AGAGTCTGGA GAAGTTCATC 
CGTTCTTCGC CCTATTTCAT GACTCTCGAT TTCGTTCGCC ACCTGGGAGA GAATCTCCCT 
GACGTCCTTC CGGGCATGCT CCCTACCTTG GTTCAAGAGG CACAGCAGCT CGCCTATTTC 
CAGAAGCAGG TCGGCCGTGT TGTTCGCCAG ATCGTTCACA AGTACCCTCG CATGAACATC 
CTTGGCCTGA CTGATCCCGA ATTGGGCTTG ACCGAGCATC TCATGGCAGC ACTCGATGGA 
TCGTTCCTTA GCTACCGCAT TGGTGGCAAG CCTGAGACAA ACCTCAAGGA GCGTTCTCCT 
CTCGCCGAAG GGATTCGCAA GAAGATCATG ATTGACGAGA TTGACTTCGA GGGCGATGCT 
GAATCTGTCG GAGGACCTTA TGATCTTGTG GTTCTCAACA CGTCCATCCT TGAGGACAAG 
AAGACTCATT CCGCGTTACA GCTTATCAGG GATTCGATGC GTCCTGGCGG CTTCCTTGTT 
GTCGTCCATG CATCTCGTAC TCCACTCAAG GAGAGAATGC TTCGTTGCGC TGGCCGTACC 
TCGAGCAACG ACGGAATCGC CACACCTCCT GACTGGCCTG ATCTCATCGA GGAATGCGGA 
TTTGAGAACA CAGCTAAGAA CTCGGATATG TGGTTCCATC CTGGATTCAC CCTCAGTGTA 
AGACAGGCAA TGTCACAGAG AAAGAAGCAG CTGCTGTGGC CTCTGCATCA CGCCACCGGA 
ACTCCTCTCG TGAAGCATCT TTTGATTGTG GGTGGTAAGA AGGTCTGGAC GTCTTGCATT 
TCCTCTGGTG TCTTCCAGGA ACTTGCCTCT TTCTGTGGAA AGATCACCGC TGTTGACTCC 
CTGGAGGATA TCCACAATGA TGAATTGCTC TCATCTTGCA CTGCAGCCAT CATCCTTGGC 
GATATTGATC AGCCTATTCT TGCTGATATG ACGGCCGAGC GAATGGACAC CATTCGGTCC 
TTGCTGCGCC CCCAAATGCT CATTATGTGG GTTACTCATA ATGCCAGGTT CGACAACCCC 
GATCATGCCG CATCGCTTGG CTTCACTCGA ACGGTTACCG GTGAAGTCCC TGGCTTGCAT 
ATACAAGTGC TCGATCTCGA CACCATTGAC ACCTGTCCGG CTGTTCGCGC CGTGTCGAAG 
ACGTTCGCTC GTTTCATCGT TCATTTCCTC GGCCCCAAGA ATGAGGATGA TGAGCCCCTG 
TGGGTTCGTG AGCACGAAGT GCACGTTGAG GGTGGCCGCT TGATCGTTCC TCGCGTTCTC 
CCGTGGAAAG AGGGTAATCA GCGTGTCAAT GCGCCCCGCC GCGTGATGAC CGACACAGTC 
AACACTCTGG AAGACGTTGT CGAGGTCCTC GCTTCAGCCT CTGGCCGGTA CGAGACTCGG 
GTCGAGAAGA GCGAAGCCTA TCCTTCGCAT GTTGAATCGG CGATGCACGT GGACTACAGC 
ACTGTCAAGG TGTTCAACAT CGGTGCCATT TGCTCCGGTC ATATCTGCAT TGGCCGCAAC 
AACGAGACTG GCAAGGCTCA AGTTGCCCTC TCTAAATCGA ATAAGTCCTT TATTTCGACG 
GCCTCTACAA TCTTCAGCAC GCTCCCTGAA GGCACTGTCG GCGTCTGTGA GCCGCTCTAC 
CTTAGCTTGC TCAGCCGGTA TATTTACGCT CTCACTATCG TCAACATGGC TAATGGCAGG 
TCTGTCATGA TCATTGAGCC TGATGGTCTC CTCTTCCAAT GCGCCAAGGA AGTTTGTAGC 
AACCGCGGCA TCTGGTTTAT GGGTGCTTCC ACGGATGAGC GTCGTAGCCG AGTTGTTCCC 
GATATTGCCT TTGTTCATTC ACAGTCAACT TCCAGGCAGA TCAAGGCCCT CTATCCCCTC 
GACGGGGCTT GCATTGTCAA CATGCTGCCC AAGACTCACG ACGTCTCTCG CATGCTTGTT 
GACTCGGTGC CTGAGAATTG CGAGTATCTC ACTCAGGATA AACTTATCTC GACTCACTTC 
ACGGCGCACC CCAACGCGGC ATTCGTTCAG AAGCTATGGG AAGAGGCTGT GACACTTTCT 
TACAACAGCA TGAAGAGCAT GCTGCTTAAT GCCATCGAGC CTTCTTTCGA CATCCTGGAC 
CCCATCACTT TGCCCAAGTT GCTTGACTCT GCTGTGTCTT CTGAGGCTTT CCAGATGCTC 
GACTGGAAGG CCGAGAGGTC TATCGCTCAC ATGATCAAGC CTATCGTGGG TGAGAAGATG 
CTTCGCCCTA ACAGTACCTA TGTCCTGGTT GGCATCACTC GCGACATGGG CCAGAGTCTC 
TGCTCTCTCT TCATCGAGCA GGGTGCTCGC CATCTTGTCT TGTGCAGTCG AAACCCCCCG 
AAGGAGCAGC CTCAGTGGCA GAAGGAGCTC AATGCTCGCG GCATCAAGGT CAAGTTCGAG 
GCGATGGATG TTACCAACCT CAACACGGTG ATTGCCTTCA AGACGAAGCT TGCCGAAGAT 
TCTTGGCCGT CTGTCGGGGG CGTGGTCAAC GGTGCCATGG TTCTTGACGA CCGTGTGTTC 
TCCGAGATGT CCGTTGAGAC TTGGAATCGC GTCATGAATC CAAAGGTGGT CGGTTCGAGG 
AATCTCGACA TAGTCTTCAA CTCTCCGGAT ATGGATTTCT TTATCATGAC CTCGTCTTTC 
GCTGCCATTG GCGGCCATGC CGGTCAATCG AACTATGCCG CGGCTAACAT GTACATGAAC 
GGCATTGCCG CTTCTCGTCG TTGCCGCGGA CTGCCGGCCA CGGCTCTGAA CATTGGGGTC 
GTCTACGGCC TTGGCTTCTT GCACCGTGAG AAGGACGATC TGTACGCCGG TCTCGAGCGT 
GAGGGATACC CGCCCATTTC CGAGCGTGAT CTTCATCACA TGTTCCTCGA GGCCATTGCT 
GCTGGAAAGC CCGTGGGACA CACCGTCTAC GACATCACTA CGGGCATGAA CCGATTCCGC 
ATGGACGCAC CGAATCAGCA CTGGCAGCTC GATCCTCGTT TCTCACACAA TACCCTCCCC 
GAGGAGGACG AGACCGCCGC TGGTTCTGCC GGCCAGCAGA TCAAGTCTCT TGCCGAGGAT 
CTTGATGCTG CCACCACCAA AGAGGACGTG ATGAAGGTGT ACGTGCCTGC CTTTATTGGC 
CGCTTGGAGG CACTTCTGCA GATTGCCGAG GGCGAAATCA CACCTGCCAA CAGCGTCACT 
GAGCTTGGTG TCGACTCTCT TATCGCAGTC GAAATCCGCT CCTGGATTTG GAAGACGGTC 
GCCCAAGATG TTGCGGTGAT GAAGATCCTT AGCGCTACCA GCATTGCCCA GTTGTGCGAG 
GGAATTGCCA GTGATCTTTT GGTCGCCCGC GGTGCTGCAG CCAAGGATTC TACTGCTCCT 
ACTGAACAGA AGGAGACTCC CGCTGGCTCT CAGACTTTGC AGGCTGTCAG TGGCTCGGTC 
AAATCAGCTC CGAGCACCAC TCCATCTCTC GAGGCTTCGG CTAGCACTTT GACTGAAGCC 
ACTGTGGTTT CGATTGTCAG CACTTCCACT GTGGTATCCA GCGCCAGCAC TGCTACCAGC 
GTTGATGGTG ACGCTGAGGA GGATAACGCC TTGTCGGCTA GTTGGGAGGA CGTAGGAAAG 
AAGGAGTCTT CCATTTTTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pks-4 ( XM_011396977.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011395279.1 Neurospora crassa OR74A polyketide synthase 4 (pks-4), mRNA.

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CloneID	ONf01857
Clone ID Related Accession (Same CDS sequence)	XM_011396978.1 , XM_011396977.1
Accession Version	XM_011396977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	polyketide synthase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507).

ATGTCTTACA AGATTCCGCT GTCTGAGCCC ATCGCCATTG TGGGCAGCAG CTGCCGCTTT 
ACGGGCGAAG CCACATCCCC CGCCAAGCTG TGGGAGCTTC TGAAGGACCC CAAGGATCTT 
ACGCGCGAAG TCCCCAAGGA TCGGTTTAAC GTCGAGGGCT TCTATCACCC CGATGGTGAG 
TATCACGGCA CTACCAATTC GGCCAAGGCC TACTTCCTCG AGCAAGACCA TCGCCTCTTC 
GATGCCAGCT TCTTCAACAT CACCCCGAAG GAGGCTGAGG CCATTGATCC CCAACAGCGT 
ATGCTGCTGG AGGTAGTGTA CGAGGCTCTC GAGTCCGCCG GTTACACCCT CCAAGATTAC 
TCCGGCAAGA AGGTTGCCGT CTTCGCTGGT GTCATGACAG CCGATTACGA CACCCTTTCT 
CAGCGCGACG ATCTGTCTGT CAGCCAGTAC TACGCCACTG GCAATGCTCG CTCCATCATT 
TCGAACCGCG TGTCGTACTT CTTCAACTTC CATGGTCCTT CTATGACCAT CGACACAGCC 
TGCTCTTCCA GTCTGGTCGC CCTTCACCAA GCCGTTCTCT CTCTTCGCTC GGGTGAGGCT 
GAGATGGCAT GCGTTTCAGG TGTCAACTTG ATTCTCACCC CCGAGCAGTT CGTCGTCGAG 
TCGAGCCTGC ACATGCTTAG TCCCACTGGC CGCTGCCACA TGTGGGACGA TCGCGCCGAC 
GGTTATGCAC GTGGCGAGGG TGTCGCCGCG ATCTTCATCA AGCCTCTTAG CAAGGCTTTG 
GCCGACGGTG ATCGTATTGA GGCCATCATC CGCGAGACCG GTGTCAACTC TGACGGCCGC 
AGCAAGGGCA TCACCATGCC CAACTGGGAG GCACAGTCCG CCCTGATCCA AGACACCTAT 
CGTCGCTCTG GCTTGAACGC CAGAGACCCC ATTGATCGTT GCCAATTCTT CGAGGCTCAT 
GGTACCGGCA CTGCTGCTGG TGATCCCAAT GAGGCTAGAG CGATTGAGGA TGCCTTTTTC 
GGCCGTAACG TCAGCACTTC GAGCCAAGAC ACGGCCGAAG TGTCAAAGCT ACTTGTGGGC 
TCTGTCAAGA CTGTCATCGG TCACACCGAA GGTGCCGCCG GATTGGCCGG TCTCTTCAAG 
GTGGTTCATG CCATGATCAA CGGCACTGTG CCTCCTAATT TGCACTTCAA CAGACTTCAG 
CCAGCTGTCG CCAAGTATTA CTCGCACCTT CATGTGCCCA CCAAGGCCCT TGCCTGGCCT 
TCTGTCGCGG CTGGACAGCC CAGACGTGCC ACTGTTAATT CCTTCGGATT CGGAGGAACC 
AACTCGACTG CAATTTGCGA AATGTACGTT CCGGAGATTC ATGACCCGGT CGCCAAGATC 
TTTGCTCCGA CCCTCATTGT GCCCAAGCTC ATCAATTCGG GCTCTCTTTC GGCTGATGGC 
CAGACTCGTC TGCCCCTGGT CCTTTCCGCC AGCTCGCCCA GGTCTATGGG CGCTGTTGCC 
AAGTGCTATC GCGATTACCT CGCGCAGGTG TCGTTGCCTC TTGAGGAGGC TGCGTGGTAT 
GCTTACAAGA ACCGCACCGC ATTTCCTTAC CGCACTGCCG TTAGCGGATC TTCACTGTCG 
GACATTATGA AGGGTCTCGA CACCTTGATT GCAAAGGCCG AGGCTGGCAA AGCAGCTACA 
ATCAGTGTCC GCGCTCGCCC CGAGACCGAT GTTCCCAAGA TCCTGGGTAT CTTTACCGGC 
CAAGGTGCTC AGTGGGCCAC CATGTCCCGT GGTTTACTGC AGAGCAACAA TGTTTATGCT 
CAGTCCATTC GCAACCTCGA CCAGGTCCTT CAGGCCTGTC CTCATCCGCC CTCGTGGTTC 
CTTGAACAGG AGATCATGGC CGACGCAAGT CTCTCTAGAG TTGGGAAGGC GGCCATCTCC 
CAGCCTCTCT GCACCGCGGT CCAGATCGCC CTCATCGATC TTCTCGCTCA CTTGGGCATT 
ACTTTCCACA CCGTTGTCGG TCATTCATCC GGCGAAATTG CTGCGGCTTA CGCGGCGGGC 
AAACTTTCTG CTCGCGATTC GATTCTCATC TCGTATTATC GCGGAAAATA CGCCCACCTC 
GCCAGCGGTG CCCAGGGCAG TCTGGGTGGC ATGATGGCTG CAGGTTTGAC GAAGGCAGAA 
GCCGAAGAGC TTTGCGCCCT GCCTCAGTTT GGCAACCGCG TTTGCGTTGC TGCCAGCAAC 
GGACCCAGCA GTGTCACCTT GTCAGGCGAC CTTGACATTG TGCAAAAGGT CCATGATCAC 
CTTGTTGACG AGAAGAAGTT TGCGAGGATG CTGCAGGTCG ACACAGCCTA TCACTCGCCT 
CACATGGTGC GCCCTGCTAA TGAGTACATT GAGGCCCTCA AGGCTTGCAA CATTGCTCCA 
TTGCAGGATG GCAACGGCAC CACCTGGGTG TCCAGCGTCT ACGGTCATGG TGAACCCACG 
TCTGAAGAGC TCGCGTCCTC CTACTGGCGC GACAACATGG TCAATGCAGT CCTTTTCTTT 
GAGGCTGTCA GCACTGCTCT GGAAGGACAA GGTCCCTTTG ACTGTGCCAT CGAGGTTGGA 
CCTCACGCGG CCCTGCGCGG CCCCGTCATT CAGACCATGA AGGCCAAGTC GGGAGAGGCC 
ATTATCTACT CTGGTCTTCT CGACAGAAAG CTCGACGATC GCATTGCTTT TGCGGACTTC 
TTGGGCTGGA TGTGGTCTAA CTTCGGCTCA TCGTCATCAT TGATTGAGAG GTTTGTTCAG 
AGCTCAGTCA ATCCTGAATT GGCTCACCTT CGTTTCCAGC AGGGTCCCAA TTACCCTTGG 
GACCACTCGC ATACCTACTA CCGCGAGTCA CGCATTTCTC GTCAGTATCA CTTCAGGACC 
CACAAACCTC ATGAGCTTCT CGGTATTCGT ACCAGGGATG ACAACGAGCA TGAGCTCAGA 
TGGCGCAACA TCATGAAGTA CGCCAAGCTT CCATGGGCCC AGGGCCACAA GTTCCAAGGA 
CAGGCGCTGC TTCCGGCTTC AGCATACTGT ATCATGGTGT TGGATGCTGC ACGCGTCCTT 
TCGGGTAACC GCGCCGCTTC TGTTGTCGAG CTGCGCGACT TGAAGTTTAT GGCTGGCATC 
ACGCTGGAGC CTGAGTCTCC CGGTGTTGAG ACCCTCTTCT CTTTGGTGGT GTCCAACTCG 
CCCAAGAACG CCTCCACTAT TGAGGCCTTA TTCACGCTGA CCTCGGCGCC TGCTGATGGC 
ACCATTGACA TGAAGCTGAA TTTCAGTGGA CAGCTTCATA TTGTCCTCGG AGAGAAGTCT 
CCCAACGCAC TTAGCCGTCG TCCCGCGCAG CGCGCTGAGA CCCTCACCGC CCAACCCGAG 
GCGTTCTACA AGATGATGGC AGGCACAGGT CTGGATTATA ACGGACCTTT CAAGGGATTG 
ACGGCCATTG AGCGTCGCTA CCAGTTTTCC TCTGGCACTG TGAAGAGGTT CCATGCAGAT 
GACCAGACTG GCTTGGACAT CAGCCCTGCC ACGCTGGACA GCTGCTTGCA GACTGCGTTC 
CTCACTGTTT CTTCCCCTGG TGACGGGTCC CTGTGGACTT CGTTCCTTCC CAAGGTGATG 
AGTCGTGTCA AGTTCAACCT TGCTACCTGT CGCAGCGGTG GGCGCGATGA TAACCTTGTT 
GTTGATGCCT ATCTTACTAA AGGCACTCCT TTCTCCAAAA CGGCTGCAGC CTCTTTCACA 
GCTGAGATCA ACATCTTCAA CCCCGAGGGC AACCTGGAGA TTCAAATTGA GGGACTGACC 
GTTGGCTCCT TCACATCGAC TCGCCCTGAA AATGACAAGG AGCTTTATCT CACTACCGTC 
TACGAACTCG ACCCTGAGGA TGAGATCGTT GCTTCTCCAG CGGAGGCCCT CAATGGCGTG 
AGTCCCATGC TCTTCGAGAG CTGTGCCAGA GTTGCCTCGT TTTACGTCGA GCGTGCCGCG 
CTTAAACGCC TCAAGGAAAC GCCCAGGGAT CTGCTTGAGC CAGCGGGTTA CCAGACCGAG 
ACTCGCTTGG TGTCCAACTC CTGGCCCAAC GAGACCGAGC AGAGTCTGGA GAAGTTCATC 
CGTTCTTCGC CCTATTTCAT GACTCTCGAT TTCGTTCGCC ACCTGGGAGA GAATCTCCCT 
GACGTCCTTC CGGGCATGCT CCCTACCTTG GTTCAAGAGG CACAGCAGCT CGCCTATTTC 
CAGAAGCAGG TCGGCCGTGT TGTTCGCCAG ATCGTTCACA AGTACCCTCG CATGAACATC 
CTTGGCCTGA CTGATCCCGA ATTGGGCTTG ACCGAGCATC TCATGGCAGC ACTCGATGGA 
TCGTTCCTTA GCTACCGCAT TGGTGGCAAG CCTGAGACAA ACCTCAAGGA GCGTTCTCCT 
CTCGCCGAAG GGATTCGCAA GAAGATCATG ATTGACGAGA TTGACTTCGA GGGCGATGCT 
GAATCTGTCG GAGGACCTTA TGATCTTGTG GTTCTCAACA CGTCCATCCT TGAGGACAAG 
AAGACTCATT CCGCGTTACA GCTTATCAGG GATTCGATGC GTCCTGGCGG CTTCCTTGTT 
GTCGTCCATG CATCTCGTAC TCCACTCAAG GAGAGAATGC TTCGTTGCGC TGGCCGTACC 
TCGAGCAACG ACGGAATCGC CACACCTCCT GACTGGCCTG ATCTCATCGA GGAATGCGGA 
TTTGAGAACA CAGCTAAGAA CTCGGATATG TGGTTCCATC CTGGATTCAC CCTCAGTGTA 
AGACAGGCAA TGTCACAGAG AAAGAAGCAG CTGCTGTGGC CTCTGCATCA CGCCACCGGA 
ACTCCTCTCG TGAAGCATCT TTTGATTGTG GGTGGTAAGA AGGTCTGGAC GTCTTGCATT 
TCCTCTGGTG TCTTCCAGGA ACTTGCCTCT TTCTGTGGAA AGATCACCGC TGTTGACTCC 
CTGGAGGATA TCCACAATGA TGAATTGCTC TCATCTTGCA CTGCAGCCAT CATCCTTGGC 
GATATTGATC AGCCTATTCT TGCTGATATG ACGGCCGAGC GAATGGACAC CATTCGGTCC 
TTGCTGCGCC CCCAAATGCT CATTATGTGG GTTACTCATA ATGCCAGGTT CGACAACCCC 
GATCATGCCG CATCGCTTGG CTTCACTCGA ACGGTTACCG GTGAAGTCCC TGGCTTGCAT 
ATACAAGTGC TCGATCTCGA CACCATTGAC ACCTGTCCGG CTGTTCGCGC CGTGTCGAAG 
ACGTTCGCTC GTTTCATCGT TCATTTCCTC GGCCCCAAGA ATGAGGATGA TGAGCCCCTG 
TGGGTTCGTG AGCACGAAGT GCACGTTGAG GGTGGCCGCT TGATCGTTCC TCGCGTTCTC 
CCGTGGAAAG AGGGTAATCA GCGTGTCAAT GCGCCCCGCC GCGTGATGAC CGACACAGTC 
AACACTCTGG AAGACGTTGT CGAGGTCCTC GCTTCAGCCT CTGGCCGGTA CGAGACTCGG 
GTCGAGAAGA GCGAAGCCTA TCCTTCGCAT GTTGAATCGG CGATGCACGT GGACTACAGC 
ACTGTCAAGG TGTTCAACAT CGGTGCCATT TGCTCCGGTC ATATCTGCAT TGGCCGCAAC 
AACGAGACTG GCAAGGCTCA AGTTGCCCTC TCTAAATCGA ATAAGTCCTT TATTTCGACG 
GCCTCTACAA TCTTCAGCAC GCTCCCTGAA GGCACTGTCG GCGTCTGTGA GCCGCTCTAC 
CTTAGCTTGC TCAGCCGGTA TATTTACGCT CTCACTATCG TCAACATGGC TAATGGCAGG 
TCTGTCATGA TCATTGAGCC TGATGGTCTC CTCTTCCAAT GCGCCAAGGA AGTTTGTAGC 
AACCGCGGCA TCTGGTTTAT GGGTGCTTCC ACGGATGAGC GTCGTAGCCG AGTTGTTCCC 
GATATTGCCT TTGTTCATTC ACAGTCAACT TCCAGGCAGA TCAAGGCCCT CTATCCCCTC 
GACGGGGCTT GCATTGTCAA CATGCTGCCC AAGACTCACG ACGTCTCTCG CATGCTTGTT 
GACTCGGTGC CTGAGAATTG CGAGTATCTC ACTCAGGATA AACTTATCTC GACTCACTTC 
ACGGCGCACC CCAACGCGGC ATTCGTTCAG AAGCTATGGG AAGAGGCTGT GACACTTTCT 
TACAACAGCA TGAAGAGCAT GCTGCTTAAT GCCATCGAGC CTTCTTTCGA CATCCTGGAC 
CCCATCACTT TGCCCAAGTT GCTTGACTCT GCTGTGTCTT CTGAGGCTTT CCAGATGCTC 
GACTGGAAGG CCGAGAGGTC TATCGCTCAC ATGATCAAGC CTATCGTGGG TGAGAAGATG 
CTTCGCCCTA ACAGTACCTA TGTCCTGGTT GGCATCACTC GCGACATGGG CCAGAGTCTC 
TGCTCTCTCT TCATCGAGCA GGGTGCTCGC CATCTTGTCT TGTGCAGTCG AAACCCCCCG 
AAGGAGCAGC CTCAGTGGCA GAAGGAGCTC AATGCTCGCG GCATCAAGGT CAAGTTCGAG 
GCGATGGATG TTACCAACCT CAACACGGTG ATTGCCTTCA AGACGAAGCT TGCCGAAGAT 
TCTTGGCCGT CTGTCGGGGG CGTGGTCAAC GGTGCCATGG TTCTTGACGA CCGTGTGTTC 
TCCGAGATGT CCGTTGAGAC TTGGAATCGC GTCATGAATC CAAAGGTGGT CGGTTCGAGG 
AATCTCGACA TAGTCTTCAA CTCTCCGGAT ATGGATTTCT TTATCATGAC CTCGTCTTTC 
GCTGCCATTG GCGGCCATGC CGGTCAATCG AACTATGCCG CGGCTAACAT GTACATGAAC 
GGCATTGCCG CTTCTCGTCG TTGCCGCGGA CTGCCGGCCA CGGCTCTGAA CATTGGGGTC 
GTCTACGGCC TTGGCTTCTT GCACCGTGAG AAGGACGATC TGTACGCCGG TCTCGAGCGT 
GAGGGATACC CGCCCATTTC CGAGCGTGAT CTTCATCACA TGTTCCTCGA GGCCATTGCT 
GCTGGAAAGC CCGTGGGACA CACCGTCTAC GACATCACTA CGGGCATGAA CCGATTCCGC 
ATGGACGCAC CGAATCAGCA CTGGCAGCTC GATCCTCGTT TCTCACACAA TACCCTCCCC 
GAGGAGGACG AGACCGCCGC TGGTTCTGCC GGCCAGCAGA TCAAGTCTCT TGCCGAGGAT 
CTTGATGCTG CCACCACCAA AGAGGACGTG ATGAAGGTGT ACGTGCCTGC CTTTATTGGC 
CGCTTGGAGG CACTTCTGCA GATTGCCGAG GGCGAAATCA CACCTGCCAA CAGCGTCACT 
GAGCTTGGTG TCGACTCTCT TATCGCAGTC GAAATCCGCT CCTGGATTTG GAAGACGGTC 
GCCCAAGATG TTGCGGTGAT GAAGATCCTT AGCGCTACCA GCATTGCCCA GTTGTGCGAG 
GGAATTGCCA GTGATCTTTT GGTCGCCCGC GGTGCTGCAG CCAAGGATTC TACTGCTCCT 
ACTGAACAGA AGGAGACTCC CGCTGGCTCT CAGACTTTGC AGGCTGTCAG TGGCTCGGTC 
AAATCAGCTC CGAGCACCAC TCCATCTCTC GAGGCTTCGG CTAGCACTTT GACTGAAGCC 
ACTGTGGTTT CGATTGTCAG CACTTCCACT GTGGTATCCA GCGCCAGCAC TGCTACCAGC 
GTTGATGGTG ACGCTGAGGA GGATAACGCC TTGTCGGCTA GTTGGGAGGA CGTAGGAAAG 
AAGGAGTCTT CCATTTTTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011395279.1
CDS	3034..10737
Translation	MSYKIPLSEPIAIVGSSCRFTGEATSPAKLWELLKDPKDLTREVPKDRFNVEGFYHPDGEYHGTTNSAKAYFLEQDHRLFDASFFNITPKEAEAIDPQQRMLLEVVYEALESAGYTLQDYSGKKVAVFAGVMTADYDTLSQRDDLSVSQYYATGNARSIISNRVSYFFNFHGPSMTIDTACSSSLVALHQAVLSLRSGEAEMACVSGVNLILTPEQFVVESSLHMLSPTGRCHMWDDRADGYARGEGVAAIFIKPLSKALADGDRIEAIIRETGVNSDGRSKGITMPNWEAQSALIQDTYRRSGLNARDPIDRCQFFEAHGTGTAAGDPNEARAIEDAFFGRNVSTSSQDTAEVSKLLVGSVKTVIGHTEGAAGLAGLFKVVHAMINGTVPPNLHFNRLQPAVAKYYSHLHVPTKALAWPSVAAGQPRRATVNSFGFGGTNSTAICEMYVPEIHDPVAKIFAPTLIVPKLINSGSLSADGQTRLPLVLSASSPRSMGAVAKCYRDYLAQVSLPLEEAAWYAYKNRTAFPYRTAVSGSSLSDIMKGLDTLIAKAEAGKAATISVRARPETDVPKILGIFTGQGAQWATMSRGLLQSNNVYAQSIRNLDQVLQACPHPPSWFLEQEIMADASLSRVGKAAISQPLCTAVQIALIDLLAHLGITFHTVVGHSSGEIAAAYAAGKLSARDSILISYYRGKYAHLASGAQGSLGGMMAAGLTKAEAEELCALPQFGNRVCVAASNGPSSVTLSGDLDIVQKVHDHLVDEKKFARMLQVDTAYHSPHMVRPANEYIEALKACNIAPLQDGNGTTWVSSVYGHGEPTSEELASSYWRDNMVNAVLFFEAVSTALEGQGPFDCAIEVGPHAALRGPVIQTMKAKSGEAIIYSGLLDRKLDDRIAFADFLGWMWSNFGSSSSLIERFVQSSVNPELAHLRFQQGPNYPWDHSHTYYRESRISRQYHFRTHKPHELLGIRTRDDNEHELRWRNIMKYAKLPWAQGHKFQGQALLPASAYCIMVLDAARVLSGNRAASVVELRDLKFMAGITLEPESPGVETLFSLVVSNSPKNASTIEALFTLTSAPADGTIDMKLNFSGQLHIVLGEKSPNALSRRPAQRAETLTAQPEAFYKMMAGTGLDYNGPFKGLTAIERRYQFSSGTVKRFHADDQTGLDISPATLDSCLQTAFLTVSSPGDGSLWTSFLPKVMSRVKFNLATCRSGGRDDNLVVDAYLTKGTPFSKTAAASFTAEINIFNPEGNLEIQIEGLTVGSFTSTRPENDKELYLTTVYELDPEDEIVASPAEALNGVSPMLFESCARVASFYVERAALKRLKETPRDLLEPAGYQTETRLVSNSWPNETEQSLEKFIRSSPYFMTLDFVRHLGENLPDVLPGMLPTLVQEAQQLAYFQKQVGRVVRQIVHKYPRMNILGLTDPELGLTEHLMAALDGSFLSYRIGGKPETNLKERSPLAEGIRKKIMIDEIDFEGDAESVGGPYDLVVLNTSILEDKKTHSALQLIRDSMRPGGFLVVVHASRTPLKERMLRCAGRTSSNDGIATPPDWPDLIEECGFENTAKNSDMWFHPGFTLSVRQAMSQRKKQLLWPLHHATGTPLVKHLLIVGGKKVWTSCISSGVFQELASFCGKITAVDSLEDIHNDELLSSCTAAIILGDIDQPILADMTAERMDTIRSLLRPQMLIMWVTHNARFDNPDHAASLGFTRTVTGEVPGLHIQVLDLDTIDTCPAVRAVSKTFARFIVHFLGPKNEDDEPLWVREHEVHVEGGRLIVPRVLPWKEGNQRVNAPRRVMTDTVNTLEDVVEVLASASGRYETRVEKSEAYPSHVESAMHVDYSTVKVFNIGAICSGHICIGRNNETGKAQVALSKSNKSFISTASTIFSTLPEGTVGVCEPLYLSLLSRYIYALTIVNMANGRSVMIIEPDGLLFQCAKEVCSNRGIWFMGASTDERRSRVVPDIAFVHSQSTSRQIKALYPLDGACIVNMLPKTHDVSRMLVDSVPENCEYLTQDKLISTHFTAHPNAAFVQKLWEEAVTLSYNSMKSMLLNAIEPSFDILDPITLPKLLDSAVSSEAFQMLDWKAERSIAHMIKPIVGEKMLRPNSTYVLVGITRDMGQSLCSLFIEQGARHLVLCSRNPPKEQPQWQKELNARGIKVKFEAMDVTNLNTVIAFKTKLAEDSWPSVGGVVNGAMVLDDRVFSEMSVETWNRVMNPKVVGSRNLDIVFNSPDMDFFIMTSSFAAIGGHAGQSNYAAANMYMNGIAASRRCRGLPATALNIGVVYGLGFLHREKDDLYAGLEREGYPPISERDLHHMFLEAIAAGKPVGHTVYDITTGMNRFRMDAPNQHWQLDPRFSHNTLPEEDETAAGSAGQQIKSLAEDLDAATTKEDVMKVYVPAFIGRLEALLQIAEGEITPANSVTELGVDSLIAVEIRSWIWKTVAQDVAVMKILSATSIAQLCEGIASDLLVARGAAAKDSTAPTEQKETPAGSQTLQAVSGSVKSAPSTTPSLEASASTLTEATVVSIVSTSTVVSSASTATSVDGDAEEDNALSASWEDVGKKESSIFL

Target ORF information:

RefSeq Version	XM_011396977.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A polyketide synthase 4 (pks-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396977.1

ORF Insert Sequence:

ATGTCTTACA AGATTCCGCT GTCTGAGCCC ATCGCCATTG TGGGCAGCAG CTGCCGCTTT 
ACGGGCGAAG CCACATCCCC CGCCAAGCTG TGGGAGCTTC TGAAGGACCC CAAGGATCTT 
ACGCGCGAAG TCCCCAAGGA TCGGTTTAAC GTCGAGGGCT TCTATCACCC CGATGGTGAG 
TATCACGGCA CTACCAATTC GGCCAAGGCC TACTTCCTCG AGCAAGACCA TCGCCTCTTC 
GATGCCAGCT TCTTCAACAT CACCCCGAAG GAGGCTGAGG CCATTGATCC CCAACAGCGT 
ATGCTGCTGG AGGTAGTGTA CGAGGCTCTC GAGTCCGCCG GTTACACCCT CCAAGATTAC 
TCCGGCAAGA AGGTTGCCGT CTTCGCTGGT GTCATGACAG CCGATTACGA CACCCTTTCT 
CAGCGCGACG ATCTGTCTGT CAGCCAGTAC TACGCCACTG GCAATGCTCG CTCCATCATT 
TCGAACCGCG TGTCGTACTT CTTCAACTTC CATGGTCCTT CTATGACCAT CGACACAGCC 
TGCTCTTCCA GTCTGGTCGC CCTTCACCAA GCCGTTCTCT CTCTTCGCTC GGGTGAGGCT 
GAGATGGCAT GCGTTTCAGG TGTCAACTTG ATTCTCACCC CCGAGCAGTT CGTCGTCGAG 
TCGAGCCTGC ACATGCTTAG TCCCACTGGC CGCTGCCACA TGTGGGACGA TCGCGCCGAC 
GGTTATGCAC GTGGCGAGGG TGTCGCCGCG ATCTTCATCA AGCCTCTTAG CAAGGCTTTG 
GCCGACGGTG ATCGTATTGA GGCCATCATC CGCGAGACCG GTGTCAACTC TGACGGCCGC 
AGCAAGGGCA TCACCATGCC CAACTGGGAG GCACAGTCCG CCCTGATCCA AGACACCTAT 
CGTCGCTCTG GCTTGAACGC CAGAGACCCC ATTGATCGTT GCCAATTCTT CGAGGCTCAT 
GGTACCGGCA CTGCTGCTGG TGATCCCAAT GAGGCTAGAG CGATTGAGGA TGCCTTTTTC 
GGCCGTAACG TCAGCACTTC GAGCCAAGAC ACGGCCGAAG TGTCAAAGCT ACTTGTGGGC 
TCTGTCAAGA CTGTCATCGG TCACACCGAA GGTGCCGCCG GATTGGCCGG TCTCTTCAAG 
GTGGTTCATG CCATGATCAA CGGCACTGTG CCTCCTAATT TGCACTTCAA CAGACTTCAG 
CCAGCTGTCG CCAAGTATTA CTCGCACCTT CATGTGCCCA CCAAGGCCCT TGCCTGGCCT 
TCTGTCGCGG CTGGACAGCC CAGACGTGCC ACTGTTAATT CCTTCGGATT CGGAGGAACC 
AACTCGACTG CAATTTGCGA AATGTACGTT CCGGAGATTC ATGACCCGGT CGCCAAGATC 
TTTGCTCCGA CCCTCATTGT GCCCAAGCTC ATCAATTCGG GCTCTCTTTC GGCTGATGGC 
CAGACTCGTC TGCCCCTGGT CCTTTCCGCC AGCTCGCCCA GGTCTATGGG CGCTGTTGCC 
AAGTGCTATC GCGATTACCT CGCGCAGGTG TCGTTGCCTC TTGAGGAGGC TGCGTGGTAT 
GCTTACAAGA ACCGCACCGC ATTTCCTTAC CGCACTGCCG TTAGCGGATC TTCACTGTCG 
GACATTATGA AGGGTCTCGA CACCTTGATT GCAAAGGCCG AGGCTGGCAA AGCAGCTACA 
ATCAGTGTCC GCGCTCGCCC CGAGACCGAT GTTCCCAAGA TCCTGGGTAT CTTTACCGGC 
CAAGGTGCTC AGTGGGCCAC CATGTCCCGT GGTTTACTGC AGAGCAACAA TGTTTATGCT 
CAGTCCATTC GCAACCTCGA CCAGGTCCTT CAGGCCTGTC CTCATCCGCC CTCGTGGTTC 
CTTGAACAGG AGATCATGGC CGACGCAAGT CTCTCTAGAG TTGGGAAGGC GGCCATCTCC 
CAGCCTCTCT GCACCGCGGT CCAGATCGCC CTCATCGATC TTCTCGCTCA CTTGGGCATT 
ACTTTCCACA CCGTTGTCGG TCATTCATCC GGCGAAATTG CTGCGGCTTA CGCGGCGGGC 
AAACTTTCTG CTCGCGATTC GATTCTCATC TCGTATTATC GCGGAAAATA CGCCCACCTC 
GCCAGCGGTG CCCAGGGCAG TCTGGGTGGC ATGATGGCTG CAGGTTTGAC GAAGGCAGAA 
GCCGAAGAGC TTTGCGCCCT GCCTCAGTTT GGCAACCGCG TTTGCGTTGC TGCCAGCAAC 
GGACCCAGCA GTGTCACCTT GTCAGGCGAC CTTGACATTG TGCAAAAGGT CCATGATCAC 
CTTGTTGACG AGAAGAAGTT TGCGAGGATG CTGCAGGTCG ACACAGCCTA TCACTCGCCT 
CACATGGTGC GCCCTGCTAA TGAGTACATT GAGGCCCTCA AGGCTTGCAA CATTGCTCCA 
TTGCAGGATG GCAACGGCAC CACCTGGGTG TCCAGCGTCT ACGGTCATGG TGAACCCACG 
TCTGAAGAGC TCGCGTCCTC CTACTGGCGC GACAACATGG TCAATGCAGT CCTTTTCTTT 
GAGGCTGTCA GCACTGCTCT GGAAGGACAA GGTCCCTTTG ACTGTGCCAT CGAGGTTGGA 
CCTCACGCGG CCCTGCGCGG CCCCGTCATT CAGACCATGA AGGCCAAGTC GGGAGAGGCC 
ATTATCTACT CTGGTCTTCT CGACAGAAAG CTCGACGATC GCATTGCTTT TGCGGACTTC 
TTGGGCTGGA TGTGGTCTAA CTTCGGCTCA TCGTCATCAT TGATTGAGAG GTTTGTTCAG 
AGCTCAGTCA ATCCTGAATT GGCTCACCTT CGTTTCCAGC AGGGTCCCAA TTACCCTTGG 
GACCACTCGC ATACCTACTA CCGCGAGTCA CGCATTTCTC GTCAGTATCA CTTCAGGACC 
CACAAACCTC ATGAGCTTCT CGGTATTCGT ACCAGGGATG ACAACGAGCA TGAGCTCAGA 
TGGCGCAACA TCATGAAGTA CGCCAAGCTT CCATGGGCCC AGGGCCACAA GTTCCAAGGA 
CAGGCGCTGC TTCCGGCTTC AGCATACTGT ATCATGGTGT TGGATGCTGC ACGCGTCCTT 
TCGGGTAACC GCGCCGCTTC TGTTGTCGAG CTGCGCGACT TGAAGTTTAT GGCTGGCATC 
ACGCTGGAGC CTGAGTCTCC CGGTGTTGAG ACCCTCTTCT CTTTGGTGGT GTCCAACTCG 
CCCAAGAACG CCTCCACTAT TGAGGCCTTA TTCACGCTGA CCTCGGCGCC TGCTGATGGC 
ACCATTGACA TGAAGCTGAA TTTCAGTGGA CAGCTTCATA TTGTCCTCGG AGAGAAGTCT 
CCCAACGCAC TTAGCCGTCG TCCCGCGCAG CGCGCTGAGA CCCTCACCGC CCAACCCGAG 
GCGTTCTACA AGATGATGGC AGGCACAGGT CTGGATTATA ACGGACCTTT CAAGGGATTG 
ACGGCCATTG AGCGTCGCTA CCAGTTTTCC TCTGGCACTG TGAAGAGGTT CCATGCAGAT 
GACCAGACTG GCTTGGACAT CAGCCCTGCC ACGCTGGACA GCTGCTTGCA GACTGCGTTC 
CTCACTGTTT CTTCCCCTGG TGACGGGTCC CTGTGGACTT CGTTCCTTCC CAAGGTGATG 
AGTCGTGTCA AGTTCAACCT TGCTACCTGT CGCAGCGGTG GGCGCGATGA TAACCTTGTT 
GTTGATGCCT ATCTTACTAA AGGCACTCCT TTCTCCAAAA CGGCTGCAGC CTCTTTCACA 
GCTGAGATCA ACATCTTCAA CCCCGAGGGC AACCTGGAGA TTCAAATTGA GGGACTGACC 
GTTGGCTCCT TCACATCGAC TCGCCCTGAA AATGACAAGG AGCTTTATCT CACTACCGTC 
TACGAACTCG ACCCTGAGGA TGAGATCGTT GCTTCTCCAG CGGAGGCCCT CAATGGCGTG 
AGTCCCATGC TCTTCGAGAG CTGTGCCAGA GTTGCCTCGT TTTACGTCGA GCGTGCCGCG 
CTTAAACGCC TCAAGGAAAC GCCCAGGGAT CTGCTTGAGC CAGCGGGTTA CCAGACCGAG 
ACTCGCTTGG TGTCCAACTC CTGGCCCAAC GAGACCGAGC AGAGTCTGGA GAAGTTCATC 
CGTTCTTCGC CCTATTTCAT GACTCTCGAT TTCGTTCGCC ACCTGGGAGA GAATCTCCCT 
GACGTCCTTC CGGGCATGCT CCCTACCTTG GTTCAAGAGG CACAGCAGCT CGCCTATTTC 
CAGAAGCAGG TCGGCCGTGT TGTTCGCCAG ATCGTTCACA AGTACCCTCG CATGAACATC 
CTTGGCCTGA CTGATCCCGA ATTGGGCTTG ACCGAGCATC TCATGGCAGC ACTCGATGGA 
TCGTTCCTTA GCTACCGCAT TGGTGGCAAG CCTGAGACAA ACCTCAAGGA GCGTTCTCCT 
CTCGCCGAAG GGATTCGCAA GAAGATCATG ATTGACGAGA TTGACTTCGA GGGCGATGCT 
GAATCTGTCG GAGGACCTTA TGATCTTGTG GTTCTCAACA CGTCCATCCT TGAGGACAAG 
AAGACTCATT CCGCGTTACA GCTTATCAGG GATTCGATGC GTCCTGGCGG CTTCCTTGTT 
GTCGTCCATG CATCTCGTAC TCCACTCAAG GAGAGAATGC TTCGTTGCGC TGGCCGTACC 
TCGAGCAACG ACGGAATCGC CACACCTCCT GACTGGCCTG ATCTCATCGA GGAATGCGGA 
TTTGAGAACA CAGCTAAGAA CTCGGATATG TGGTTCCATC CTGGATTCAC CCTCAGTGTA 
AGACAGGCAA TGTCACAGAG AAAGAAGCAG CTGCTGTGGC CTCTGCATCA CGCCACCGGA 
ACTCCTCTCG TGAAGCATCT TTTGATTGTG GGTGGTAAGA AGGTCTGGAC GTCTTGCATT 
TCCTCTGGTG TCTTCCAGGA ACTTGCCTCT TTCTGTGGAA AGATCACCGC TGTTGACTCC 
CTGGAGGATA TCCACAATGA TGAATTGCTC TCATCTTGCA CTGCAGCCAT CATCCTTGGC 
GATATTGATC AGCCTATTCT TGCTGATATG ACGGCCGAGC GAATGGACAC CATTCGGTCC 
TTGCTGCGCC CCCAAATGCT CATTATGTGG GTTACTCATA ATGCCAGGTT CGACAACCCC 
GATCATGCCG CATCGCTTGG CTTCACTCGA ACGGTTACCG GTGAAGTCCC TGGCTTGCAT 
ATACAAGTGC TCGATCTCGA CACCATTGAC ACCTGTCCGG CTGTTCGCGC CGTGTCGAAG 
ACGTTCGCTC GTTTCATCGT TCATTTCCTC GGCCCCAAGA ATGAGGATGA TGAGCCCCTG 
TGGGTTCGTG AGCACGAAGT GCACGTTGAG GGTGGCCGCT TGATCGTTCC TCGCGTTCTC 
CCGTGGAAAG AGGGTAATCA GCGTGTCAAT GCGCCCCGCC GCGTGATGAC CGACACAGTC 
AACACTCTGG AAGACGTTGT CGAGGTCCTC GCTTCAGCCT CTGGCCGGTA CGAGACTCGG 
GTCGAGAAGA GCGAAGCCTA TCCTTCGCAT GTTGAATCGG CGATGCACGT GGACTACAGC 
ACTGTCAAGG TGTTCAACAT CGGTGCCATT TGCTCCGGTC ATATCTGCAT TGGCCGCAAC 
AACGAGACTG GCAAGGCTCA AGTTGCCCTC TCTAAATCGA ATAAGTCCTT TATTTCGACG 
GCCTCTACAA TCTTCAGCAC GCTCCCTGAA GGCACTGTCG GCGTCTGTGA GCCGCTCTAC 
CTTAGCTTGC TCAGCCGGTA TATTTACGCT CTCACTATCG TCAACATGGC TAATGGCAGG 
TCTGTCATGA TCATTGAGCC TGATGGTCTC CTCTTCCAAT GCGCCAAGGA AGTTTGTAGC 
AACCGCGGCA TCTGGTTTAT GGGTGCTTCC ACGGATGAGC GTCGTAGCCG AGTTGTTCCC 
GATATTGCCT TTGTTCATTC ACAGTCAACT TCCAGGCAGA TCAAGGCCCT CTATCCCCTC 
GACGGGGCTT GCATTGTCAA CATGCTGCCC AAGACTCACG ACGTCTCTCG CATGCTTGTT 
GACTCGGTGC CTGAGAATTG CGAGTATCTC ACTCAGGATA AACTTATCTC GACTCACTTC 
ACGGCGCACC CCAACGCGGC ATTCGTTCAG AAGCTATGGG AAGAGGCTGT GACACTTTCT 
TACAACAGCA TGAAGAGCAT GCTGCTTAAT GCCATCGAGC CTTCTTTCGA CATCCTGGAC 
CCCATCACTT TGCCCAAGTT GCTTGACTCT GCTGTGTCTT CTGAGGCTTT CCAGATGCTC 
GACTGGAAGG CCGAGAGGTC TATCGCTCAC ATGATCAAGC CTATCGTGGG TGAGAAGATG 
CTTCGCCCTA ACAGTACCTA TGTCCTGGTT GGCATCACTC GCGACATGGG CCAGAGTCTC 
TGCTCTCTCT TCATCGAGCA GGGTGCTCGC CATCTTGTCT TGTGCAGTCG AAACCCCCCG 
AAGGAGCAGC CTCAGTGGCA GAAGGAGCTC AATGCTCGCG GCATCAAGGT CAAGTTCGAG 
GCGATGGATG TTACCAACCT CAACACGGTG ATTGCCTTCA AGACGAAGCT TGCCGAAGAT 
TCTTGGCCGT CTGTCGGGGG CGTGGTCAAC GGTGCCATGG TTCTTGACGA CCGTGTGTTC 
TCCGAGATGT CCGTTGAGAC TTGGAATCGC GTCATGAATC CAAAGGTGGT CGGTTCGAGG 
AATCTCGACA TAGTCTTCAA CTCTCCGGAT ATGGATTTCT TTATCATGAC CTCGTCTTTC 
GCTGCCATTG GCGGCCATGC CGGTCAATCG AACTATGCCG CGGCTAACAT GTACATGAAC 
GGCATTGCCG CTTCTCGTCG TTGCCGCGGA CTGCCGGCCA CGGCTCTGAA CATTGGGGTC 
GTCTACGGCC TTGGCTTCTT GCACCGTGAG AAGGACGATC TGTACGCCGG TCTCGAGCGT 
GAGGGATACC CGCCCATTTC CGAGCGTGAT CTTCATCACA TGTTCCTCGA GGCCATTGCT 
GCTGGAAAGC CCGTGGGACA CACCGTCTAC GACATCACTA CGGGCATGAA CCGATTCCGC 
ATGGACGCAC CGAATCAGCA CTGGCAGCTC GATCCTCGTT TCTCACACAA TACCCTCCCC 
GAGGAGGACG AGACCGCCGC TGGTTCTGCC GGCCAGCAGA TCAAGTCTCT TGCCGAGGAT 
CTTGATGCTG CCACCACCAA AGAGGACGTG ATGAAGGTGT ACGTGCCTGC CTTTATTGGC 
CGCTTGGAGG CACTTCTGCA GATTGCCGAG GGCGAAATCA CACCTGCCAA CAGCGTCACT 
GAGCTTGGTG TCGACTCTCT TATCGCAGTC GAAATCCGCT CCTGGATTTG GAAGACGGTC 
GCCCAAGATG TTGCGGTGAT GAAGATCCTT AGCGCTACCA GCATTGCCCA GTTGTGCGAG 
GGAATTGCCA GTGATCTTTT GGTCGCCCGC GGTGCTGCAG CCAAGGATTC TACTGCTCCT 
ACTGAACAGA AGGAGACTCC CGCTGGCTCT CAGACTTTGC AGGCTGTCAG TGGCTCGGTC 
AAATCAGCTC CGAGCACCAC TCCATCTCTC GAGGCTTCGG CTAGCACTTT GACTGAAGCC 
ACTGTGGTTT CGATTGTCAG CACTTCCACT GTGGTATCCA GCGCCAGCAC TGCTACCAGC 
GTTGATGGTG ACGCTGAGGA GGATAACGCC TTGTCGGCTA GTTGGGAGGA CGTAGGAAAG 
AAGGAGTCTT CCATTTTTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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pks-4 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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