NCU08001 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU08001
Entrez Gene ID	3878835
Full Name	vacuolar protein-sorting protein bro-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_957578.2	XP_962671.1	vacuolar protein-sorting protein bro-1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	SPAC17G6.05c	NP_594252.1
Eremothecium gossypii	AGOS_AGR204W	NP_986870.2
Canis lupus familiaris (dog)	PTPN23	XP_851530.2
Magnaporthe oryzae (rice blast fungus)	MGG_15323	XP_003714762.1
Neurospora crassa	NCU08001	XP_962671.1
Saccharomyces cerevisiae (baker's yeast)	BRO1	NP_015241.1
Kluyveromyces lactis	KLLA0E23871g	XP_455027.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU08001 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU08001 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf01364	XM_957578.2 Latest version!	Neurospora crassa OR74A vacuolar protein-sorting protein bro-1 (NCU08001), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU08001 ( XM_957578.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_962671.1 Neurospora crassa OR74A vacuolar protein-sorting protein bro-1 (NCU08001), mRNA.

Price & Availability↑

CloneID	ONf01364
Clone ID Related Accession (Same CDS sequence)	XM_957578.2
Accession Version	XM_957578.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	vacuolar protein-sorting protein bro-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_957578.1.

ATGGTGCAGG CGCCCATGAT CTCGGTGCCC CTCAAGGCCA CAAGCGAGAT TGACTGGGTT 
GCTCCCCTCA AAAACTACAT TCGAAATACG TACGGCGATG ATCCGGAGCG CTATGCCGAA 
GAATGTGCGA CCCTCAACCG GTTACGCCAG GATATGCGAG GCGCCGGGAA GGACAGCACG 
TCGGGAAGGG ACCTGCTCTA TCGCTACTAC GGCCAGCTCG AACTTTTGGA TTTGAGATTT 
CCGGTGGACG AGAAGAACAT CAAGATTTCC TTTACATGGT TCGACGCTTT TACTCACAAG 
CCCACAGCGC AATATTCGCT GGCTTTTGAG AAGGCCTCCA TCATCTTCAA CATTTCCGCC 
GTTCTATCCT GCCATGCGGC ACACCAATTG CGAACCGAGG AGGCGGGACT CAAGACGGCA 
TACCACTCGT TCCAGGCCTC GGCCGGCATG TTCACCTACA TCAACGAGAA CTTCCTCCAC 
GCGCCCTCGT CCGATCTGAG CAGGGAAACG GTCAAGACGC TAATCAGCAT CATGTTGGCC 
CAGGCGCAAG AGGTCTTCCT TGAGAAGCAG ATTGCTGATC AAAAAAAGAA TGGTCTGCTC 
GCCAAGTTGT CTAGCCAAGC AGCCGCTCTC TACGCCCAGG CTGTTGAGGG CGTTCAGGAA 
AACGTCACCA AGGCCATTTT CGAGAAAGTT TGGCTGTCTG TGGTTCAGAT CAAGCTTAAC 
TTCATGAACT CACTCGCCCA GTACTATCAA GCCCTCGCTG ATGAGGATGC AAACTCTTAT 
GGCGTGGCCA TTGCGCGTCT TGAAATTGCA CAGGGTCTTG CCAAAGAAGC AAACAAGATG 
GCACACAGTT TTCCCACCTC AGTGCCCCCG AACTCGAATT TGACTTCGGA CTGTGGCCAC 
ATCCTGGCAG ATGCGACCAA AAGACACCTC GCAACTGTCA AGGAAAAGTT GGAGGAACTC 
AACAAGGAAA ATGATATGAT CTACCATCAA CCCGTACCTG CTGAGGCCAG CGTTGCACCA 
GTCCCCAAGC TGCCGGCTGC GAAACCGATA CCCGTTAGCG AACTATATGC CGGCCAGGAT 
ATTCAACGGA TCACCGGCCC CGACCTATTC GCGAAAATTG TGCCTCTCGC GGTCACCGAA 
TCGGCTAGCT TGTACGATGA AGAGAAGGCC AAGCTGGTGA GAGCTGAGAC GGAAAGAGTA 
GAAACCGCAA ACAGCGAGAT GGCGGCTAGC CTGGACTATC TCAGGCTTCC GGGCGCTCTT 
CAGGTTTTGA AGGGAGGGTT CGATCAGGAT ATCCTTCCTG ACGAAGACTT TAGGACATGG 
TGTGTGGATG TCGCGGATCA CGAAAGCCCT CATAGGATTT TCGAATATCT TCATACCGAA 
AAGCAGGCCA TTTCAACAAT ACTGGATAAG AGTAGCAGGC AGTTGGATAT GGAGGAAAGC 
GTGTGTGAGA AGATGCGTTC CAAGTACGAT GCGGAATGGA CTCAGCAGCC GAGTTCGCGG 
TTGACCACTA CGCTCAGAAC GGACATTAGG AGGTATAGGG AGGCCTTGGA AGTGGCAGCG 
AAGAGTGATG GGCAGCTCGC GACAAAGCTT AGAGCCAACG AAACTGAGTT GGATGAGATG 
CGCCAGGCCG CGCAGCATGG AGAGATCGAT GAGTTGTTCC AGAGAGCAGT CAGGAAGTCG 
CGAAAGTCAA ACCCGAATAG CCCCGCCACC GTGGAGCCTA ATCTTTTGGA GGCCGATTTT 
GATGATGGTG GCCCGAGTGT GGTTGAGCAG ATCCAAAAGG TGGAAGATAT TCTGAAGAAG 
CTCAGCTTGG TGAAGAAGGA GCGTCTGCAG GTACTACAGG ATCTGAAGCA AAAGGCGCAC 
AGTGATGATA TCTCCCAAAT TCTTATTCTC AACAAGAAAT CGATTGCAAA CTACGAGCAG 
CAACTCTTTC AACAGGAACT GGAGAAGTTC AGGCCTCATC AGAACCGCCT CGTGCAAGCT 
TCACACAAGC AGGCTGCTCT CATGAGGGAG CTTACAGTAA CATTTAACAA CCTCCTTCAG 
GACAAGCGAG TGCGTGCCGA CCAAAGCCGA TACGAGTCGG TACAACGATC GCGCACCTCC 
GTCATCAACA AGTACAAGCG TGCCTACCAA GAGTTCTTGG ACCTTGAGGC GGGTCTGCAG 
AGTGCCAAGA ACTGGTATAA GGATATGCGA CAGGAGGCCG AGAGCCTGGA GAAGAACGTG 
GAGGCGTTCG TCAATAACCG TAGAGCAGAA GGTGCCCAGC TTCTCAACCA GATCGAGCAG 
GACCGGGCTG CCAACAAGAG CTCCCACGCC GCGCTGGAAC AAGAGCGGTT GAAGAATCTC 
ATGGAGCGCA TGTCGATGGA CCCATCACCG ACCTCCCCCA AGCCTTCATC GGGATCAGGA 
GGCCGACCTA CGCCCGCTCC CTTATCATTC GCCCCAGCAG CTGTCTCCAA CACGCCACTG 
TCAGCCTACC AAAAGTCCAA CTTTTCTACC CAGTATCCCG CCTCGCCGCC AGCCACACAA 
GTTCCCCACA ACCCCGGGGG CCAGCAACAG ACGCCCTATC AACAATACAA CCCCAGCTCC 
CTCGGCCGCA TCCCCGGCCC CGCCTCCCCA CCTCCGAACC AAACAAGCTT CAACATCGGC 
CCCGGCCGCC ACCCCGCCTC TCCGCCGCCG ACGCAAACCT CCTTCGCCCA ATCCCGCCCT 
TACAGCTTGA CGACGTACGG TAACCCTTCG GCACTGAATC CTCAGGGTGG GCAACCGCAG 
CAATCGCAGC CAGGAGGCTA CGTCCCTCCA GGCTTCGTCC CACCGCCGCC GCCTCCGGGA 
CCCCCGCCGC TGGGTCCGCA GCAAACGGTG CATTACGGCG GGAACGAGTA TTATGCGGGT 
GCCATGGGTA ATCCGAATAT TGGAAGGCCG GGGTCGGGGC AGCAGGGGCC ACAGGGGCAG 
CAAGGGGGTT GGGGACAGCC GCCGCCGCAA CAGCAGCTGT ATCAGCAGCA GGGAGGAGGA 
GGGGGTGATC CTTGGGCTGG GTTGAGTGCT TGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_962671.1
CDS	711..3749
Translation	MVQAPMISVPLKATSEIDWVAPLKNYIRNTYGDDPERYAEECATLNRLRQDMRGAGKDSTSGRDLLYRYYGQLELLDLRFPVDEKNIKISFTWFDAFTHKPTAQYSLAFEKASIIFNISAVLSCHAAHQLRTEEAGLKTAYHSFQASAGMFTYINENFLHAPSSDLSRETVKTLISIMLAQAQEVFLEKQIADQKKNGLLAKLSSQAAALYAQAVEGVQENVTKAIFEKVWLSVVQIKLNFMNSLAQYYQALADEDANSYGVAIARLEIAQGLAKEANKMAHSFPTSVPPNSNLTSDCGHILADATKRHLATVKEKLEELNKENDMIYHQPVPAEASVAPVPKLPAAKPIPVSELYAGQDIQRITGPDLFAKIVPLAVTESASLYDEEKAKLVRAETERVETANSEMAASLDYLRLPGALQVLKGGFDQDILPDEDFRTWCVDVADHESPHRIFEYLHTEKQAISTILDKSSRQLDMEESVCEKMRSKYDAEWTQQPSSRLTTTLRTDIRRYREALEVAAKSDGQLATKLRANETELDEMRQAAQHGEIDELFQRAVRKSRKSNPNSPATVEPNLLEADFDDGGPSVVEQIQKVEDILKKLSLVKKERLQVLQDLKQKAHSDDISQILILNKKSIANYEQQLFQQELEKFRPHQNRLVQASHKQAALMRELTVTFNNLLQDKRVRADQSRYESVQRSRTSVINKYKRAYQEFLDLEAGLQSAKNWYKDMRQEAESLEKNVEAFVNNRRAEGAQLLNQIEQDRAANKSSHAALEQERLKNLMERMSMDPSPTSPKPSSGSGGRPTPAPLSFAPAAVSNTPLSAYQKSNFSTQYPASPPATQVPHNPGGQQQTPYQQYNPSSLGRIPGPASPPPNQTSFNIGPGRHPASPPPTQTSFAQSRPYSLTTYGNPSALNPQGGQPQQSQPGGYVPPGFVPPPPPPGPPPLGPQQTVHYGGNEYYAGAMGNPNIGRPGSGQQGPQGQQGGWGQPPPQQQLYQQQGGGGGDPWAGLSAWK

Target ORF information:

RefSeq Version	XM_957578.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A vacuolar protein-sorting protein bro-1 (NCU08001), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_957578.2

ORF Insert Sequence:

ATGGTGCAGG CGCCCATGAT CTCGGTGCCC CTCAAGGCCA CAAGCGAGAT TGACTGGGTT 
GCTCCCCTCA AAAACTACAT TCGAAATACG TACGGCGATG ATCCGGAGCG CTATGCCGAA 
GAATGTGCGA CCCTCAACCG GTTACGCCAG GATATGCGAG GCGCCGGGAA GGACAGCACG 
TCGGGAAGGG ACCTGCTCTA TCGCTACTAC GGCCAGCTCG AACTTTTGGA TTTGAGATTT 
CCGGTGGACG AGAAGAACAT CAAGATTTCC TTTACATGGT TCGACGCTTT TACTCACAAG 
CCCACAGCGC AATATTCGCT GGCTTTTGAG AAGGCCTCCA TCATCTTCAA CATTTCCGCC 
GTTCTATCCT GCCATGCGGC ACACCAATTG CGAACCGAGG AGGCGGGACT CAAGACGGCA 
TACCACTCGT TCCAGGCCTC GGCCGGCATG TTCACCTACA TCAACGAGAA CTTCCTCCAC 
GCGCCCTCGT CCGATCTGAG CAGGGAAACG GTCAAGACGC TAATCAGCAT CATGTTGGCC 
CAGGCGCAAG AGGTCTTCCT TGAGAAGCAG ATTGCTGATC AAAAAAAGAA TGGTCTGCTC 
GCCAAGTTGT CTAGCCAAGC AGCCGCTCTC TACGCCCAGG CTGTTGAGGG CGTTCAGGAA 
AACGTCACCA AGGCCATTTT CGAGAAAGTT TGGCTGTCTG TGGTTCAGAT CAAGCTTAAC 
TTCATGAACT CACTCGCCCA GTACTATCAA GCCCTCGCTG ATGAGGATGC AAACTCTTAT 
GGCGTGGCCA TTGCGCGTCT TGAAATTGCA CAGGGTCTTG CCAAAGAAGC AAACAAGATG 
GCACACAGTT TTCCCACCTC AGTGCCCCCG AACTCGAATT TGACTTCGGA CTGTGGCCAC 
ATCCTGGCAG ATGCGACCAA AAGACACCTC GCAACTGTCA AGGAAAAGTT GGAGGAACTC 
AACAAGGAAA ATGATATGAT CTACCATCAA CCCGTACCTG CTGAGGCCAG CGTTGCACCA 
GTCCCCAAGC TGCCGGCTGC GAAACCGATA CCCGTTAGCG AACTATATGC CGGCCAGGAT 
ATTCAACGGA TCACCGGCCC CGACCTATTC GCGAAAATTG TGCCTCTCGC GGTCACCGAA 
TCGGCTAGCT TGTACGATGA AGAGAAGGCC AAGCTGGTGA GAGCTGAGAC GGAAAGAGTA 
GAAACCGCAA ACAGCGAGAT GGCGGCTAGC CTGGACTATC TCAGGCTTCC GGGCGCTCTT 
CAGGTTTTGA AGGGAGGGTT CGATCAGGAT ATCCTTCCTG ACGAAGACTT TAGGACATGG 
TGTGTGGATG TCGCGGATCA CGAAAGCCCT CATAGGATTT TCGAATATCT TCATACCGAA 
AAGCAGGCCA TTTCAACAAT ACTGGATAAG AGTAGCAGGC AGTTGGATAT GGAGGAAAGC 
GTGTGTGAGA AGATGCGTTC CAAGTACGAT GCGGAATGGA CTCAGCAGCC GAGTTCGCGG 
TTGACCACTA CGCTCAGAAC GGACATTAGG AGGTATAGGG AGGCCTTGGA AGTGGCAGCG 
AAGAGTGATG GGCAGCTCGC GACAAAGCTT AGAGCCAACG AAACTGAGTT GGATGAGATG 
CGCCAGGCCG CGCAGCATGG AGAGATCGAT GAGTTGTTCC AGAGAGCAGT CAGGAAGTCG 
CGAAAGTCAA ACCCGAATAG CCCCGCCACC GTGGAGCCTA ATCTTTTGGA GGCCGATTTT 
GATGATGGTG GCCCGAGTGT GGTTGAGCAG ATCCAAAAGG TGGAAGATAT TCTGAAGAAG 
CTCAGCTTGG TGAAGAAGGA GCGTCTGCAG GTACTACAGG ATCTGAAGCA AAAGGCGCAC 
AGTGATGATA TCTCCCAAAT TCTTATTCTC AACAAGAAAT CGATTGCAAA CTACGAGCAG 
CAACTCTTTC AACAGGAACT GGAGAAGTTC AGGCCTCATC AGAACCGCCT CGTGCAAGCT 
TCACACAAGC AGGCTGCTCT CATGAGGGAG CTTACAGTAA CATTTAACAA CCTCCTTCAG 
GACAAGCGAG TGCGTGCCGA CCAAAGCCGA TACGAGTCGG TACAACGATC GCGCACCTCC 
GTCATCAACA AGTACAAGCG TGCCTACCAA GAGTTCTTGG ACCTTGAGGC GGGTCTGCAG 
AGTGCCAAGA ACTGGTATAA GGATATGCGA CAGGAGGCCG AGAGCCTGGA GAAGAACGTG 
GAGGCGTTCG TCAATAACCG TAGAGCAGAA GGTGCCCAGC TTCTCAACCA GATCGAGCAG 
GACCGGGCTG CCAACAAGAG CTCCCACGCC GCGCTGGAAC AAGAGCGGTT GAAGAATCTC 
ATGGAGCGCA TGTCGATGGA CCCATCACCG ACCTCCCCCA AGCCTTCATC GGGATCAGGA 
GGCCGACCTA CGCCCGCTCC CTTATCATTC GCCCCAGCAG CTGTCTCCAA CACGCCACTG 
TCAGCCTACC AAAAGTCCAA CTTTTCTACC CAGTATCCCG CCTCGCCGCC AGCCACACAA 
GTTCCCCACA ACCCCGGGGG CCAGCAACAG ACGCCCTATC AACAATACAA CCCCAGCTCC 
CTCGGCCGCA TCCCCGGCCC CGCCTCCCCA CCTCCGAACC AAACAAGCTT CAACATCGGC 
CCCGGCCGCC ACCCCGCCTC TCCGCCGCCG ACGCAAACCT CCTTCGCCCA ATCCCGCCCT 
TACAGCTTGA CGACGTACGG TAACCCTTCG GCACTGAATC CTCAGGGTGG GCAACCGCAG 
CAATCGCAGC CAGGAGGCTA CGTCCCTCCA GGCTTCGTCC CACCGCCGCC GCCTCCGGGA 
CCCCCGCCGC TGGGTCCGCA GCAAACGGTG CATTACGGCG GGAACGAGTA TTATGCGGGT 
GCCATGGGTA ATCCGAATAT TGGAAGGCCG GGGTCGGGGC AGCAGGGGCC ACAGGGGCAG 
CAAGGGGGTT GGGGACAGCC GCCGCCGCAA CAGCAGCTGT ATCAGCAGCA GGGAGGAGGA 
GGGGGTGATC CTTGGGCTGG GTTGAGTGCT TGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU08001 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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