NCU07010 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU07010
Entrez Gene ID	3877979
Full Name	cortical actin cytoskeleton protein asp1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395914.1	XP_011394216.1	cortical actin cytoskeleton protein asp1, variant
XM_011395913.1	XP_011394215.1	cortical actin cytoskeleton protein asp1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B02805g	XP_451657.1
Schizosaccharomyces pombe (fission yeast)	asp1	NP_587877.1
Saccharomyces cerevisiae (baker's yeast)	VIP1	NP_013514.1
Magnaporthe oryzae (rice blast fungus)	MGG_06039	XP_003711888.1
Eremothecium gossypii	AGOS_AER082W	NP_984942.1
Neurospora crassa	NCU07010	XP_961831.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU07010 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU07010 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf11890	XM_011395913.1 Latest version!	Neurospora crassa OR74A cortical actin cytoskeleton protein asp1 (NCU07010), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ONf11891	XM_011395914.1 Latest version!	Neurospora crassa OR74A cortical actin cytoskeleton protein asp1, variant (NCU07010), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU07010 ( XM_011395913.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394215.1 Neurospora crassa OR74A cortical actin cytoskeleton protein asp1 (NCU07010), mRNA.

Price & Availability↑

CloneID	ONf11890
Clone ID Related Accession (Same CDS sequence)	XM_011395913.1
Accession Version	XM_011395913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	cortical actin cytoskeleton protein asp1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504).

ATGGAAGCAC ACCTCGAACC CACCTCCCTC GATCGTACGC CAGTGTCCGC CGACAGCGTG 
CCGTCCTCCG CCTCCGAAGC TTCCTCTTCC ACTCATCCGG ACCAAGACCC AGACACTCCT 
GTCGCGACAT CAGAAGCAGC AACCGGCGGC TTGGACGTGC CTGTAGAAGA AGCCGAAGAC 
ATGGCAGGCA CACCGTCGTC GAGCATCGCA GTCCCTGGCG CCATCCCCAT CACCAGTGAC 
AGCGGCATCA CAAGTACCGG AAAATCCAAA CGACGACACA GTCTCGCCTC TTCCTTTACC 
AACAGCATAG CTAGCCGAAA GAGCAGGTAC TCTGAGCCTG GCAGCCAATC TGTTCATGCT 
GATCGCGCCA ATGTGTCGAT GCCCCCTCCC GTCGCCCTGC CACAAGCATC GTCGTCATTA 
GCAAAGAGCC CGCGCGCCTC GCTCTCGCAC AGCCGCCGCG AATCGTCCAA TTCGTTCCGC 
TCCTTCATCT CGGACGCCTG GAGCGACGCA AATAACAGCC AAGGATACTC GGACTTGGGT 
GGTGGAGTAC ATGTGCCCGG CAGCTTTGGC AGCGCCTCGG CGGTCCCACC CCTGTTTTCT 
CCCCGAGATT TCGCCTTGGC CGCCGGCGAT AACGGCCAGA ATCAAGCTAC GATCGAAGAC 
TCGGAGACTC CCCGTCTACG CCCCGGGACC GGTAACACGA ACGCCGAATC ACCTCAACCA 
CCACCACCAC AACAACAACA ACAACGCCAA CTCCCCGATC CCGCTCTGCA GAATCCCACT 
ACTCCCACCC AACGCGAATA CCCCTACCAC TCCCGCCGCA GAAACTCGTC CTTCTTTGGC 
TTCGAGTACA GCCAGTCAGA CAGCAACAAG CGAAAGAGTA TTTCGTCCAT ATACTCTCTA 
GCTTCGGCCC GTGGGGTTCC CAGTTCCGAG AGCGGCAGCA CCAGCGGCGC CACCGGCCCT 
GCCCGAGTGG TATCAGGCTT GATGTCTTCC TCCGCCCCCT CCAAATCGGG AGCCATCCCA 
CCTGCCAGCA CCGCCCAGAC TGCAGAAGCC AGCGTCTCGA CAACCACCGT TACCACGGGA 
AGCCAAGGCG CGCCAGGCAC CCACCAGCTC TCGCCGCGGG AATCGCATTT TCAGCAGATG 
GGCGACTTGC TCAAGGATGG CCAGAGCAGG GGAAACCATC ACCATACTTC GCAGGCCCAC 
GGCCACGGTG TTCCTGCTCC TGTGGACACG GCTGTTGCTA ACGGCGCACC ACCCGCACCA 
CCCACACCGC GCGCAATTCC ACCCAGGTCA AGGAGCAGGG CAAAGCGCAG GTTCAGTGGT 
AGTACCGCTG CTAGCGGAAA TCAGAGTCCC AATGGTGAAA GAGTGGTATC AGGCCATGAA 
CACCGGCCTC CCAAGGAGGA GCACAAGCCT GCGCCCTACG GTGTTATCGG TGTGTGTGCC 
TTGGACGTGA AGGCGAGGAG CAAGCCCAGC AGGAATATCC TCAACCGCTT GATTCAGAAC 
GGCGAATTTG ATGTTTGTGT CTTTGGCGAC AAAGTGATTC TTGATGAAGA GGTCGAAAAC 
TGGCCCATGT GCGACTATTT AATCTCCTTT TACTCGGACG GCTTCCCCCT TGACAAGGCC 
ATCGCCTACG TCAAGGCACG CAAGCCCTTC TGCGTTAACG ATGTCCCTAT GCAAAAGATA 
CTATGGGACC GCCGCCTCTG CCTTCGCCTC TTGGACCGCA TCAATGTCCC CACCCCTCAG 
CGCATCGAAG TGAACCGGGA CGGTGGCCCT CACCTGTTGA CCCCCGAAAT TTGCAAGCTC 
ATCAAGGACG TCAGCGGTGT ACAGTTTGAG CCCACTAATA CGGATCCAGA GTACGCCAAG 
CAGGTCGCGC CGCGCAAGAT CGAGCTCCTC GAAAACGGCG ATGTTCTCTC TGTCGACGGA 
ACGCTCATCA AGAAGCCGTT TGTCGAGAAG CCTACGAGCG GTGAAGACCA CAATATCATC 
ATCTACTTTC CTTCCTACGC TGGCGGTGGT GCAAGGAAGC TTTTCCGCAA GATCGGCAAC 
AAGAGCAGTG AATATGTGGA GGATCTCAAC GTGCCCAGGT GCATCACTCA GCCCGACGAG 
AGTTTCATTT ACGAAAAGTT CATGCAGGTC GACAATGCCG AGGACGTGAA AGCGTATACT 
GTTGGGCCAA CATACTGCCA CGCTGAGACT CGCAAGTCCC CTGTTGTGGA CGGCGTCGTG 
CGAAGAAACA CGCATGGAAA AGAGGTCCGC TATGTCACAG CCTTGAGCGA CGAGGAAAAG 
GAAATTGCGA GCAAAATCAG CACAGCCTTC GGACAACGCG TTTGCGGTTT CGACTTATTG 
CGGGCGGGGG GCAAGAGTTA TGTCATTGAC GTGAACGGCT GGAGTTTCGT CAAAGATAAT 
GAGGACTATT ACAACCAGTG CGCCAAAATC CTCAGGGAAA TGTTTATCGC CGAGAGGCAG 
CGCAGGGGTG GAATACCTTC GCCGTGCCCG TCTCCCGCCA TCTCCGAGGT GCCTGATCCT 
CTTGCCGCTC GCGCAGCTCT CATGAACAAG GAGAAGGAAC TCAGCGCGTT GGCGGCAGCC 
CAGGGTAAGG TGAGCTTGGA CAAGCAGAGG TCGGACAACA CCAGACACCA CGATGCGCCA 
CCGCACGTCA AGTCAGATAC CTCGCTTGTC TCTAAGCTGT CAACCAAGCC GAGCAGCCCG 
CGCAACAGTC AGTACGGAGA GAGTGCACCA CCCACAGCGC CATCAACGCT GCCCACTACC 
CCTTCGCTCA CTTCTGTCGC TATTGCTGAA CAGGAGCATG AGCATGAGGA GACACCGCCG 
CCGCCTCCCC CGAAGCCTAG CTGGAAGCTC AAGGGTGTGG TATCTGTCAT CCGCCACGCT 
GATCGTACGC CGAAGCAGAA GTACAAATTC ACGTTCCACA CAGATCCCTT CATTCAGCTA 
CTCAAGGGTC ACCAAGAAGA AGTGCTCCTG ATTGGTGAGC CGGCGCTGGC AAGTGTACTT 
GATGCTGTTG ATGTGGCCCT GAAGGCTGGT ATTGAAGACC CCGTCAAGCT CAAGTCACTC 
CGCAACGTCC TTGTGAAGAA GGGCGGATGG GCTGGTACCA AGGTCCAAAT AAAGCCAATG 
TTCAGGAAGA AAGACAAGCC CAAGAAGGAA GACAAAGAGA AGGAGGGCGA AAAGCCTGAC 
CAAGAGGCCA AGGAGGATGC TGCACCCGGT GAAGGTGACA GTGTCGACAA GGACGGTAAC 
TCCAAGCCTC GTAGGCCAGT CAAGAGGCAC GATTCCTTGT CAGGCGTTAC CATGTCCAAG 
TTCACTGCTG CCGAGGAGAG CTTGGTGCTG GATAAGCTTC AGCTCATTGT GAAATGGGGC 
GGCGAGCCGA CACACTCCGC CCGCTACCAG GCACAGGAAC TCGGTGAGAG CATGCGTAGT 
GATCTTGGTC TCATGAACCG TGATATCCTG GAGGAGGTCC ACGTCTTCAG CAGCAGCGAG 
CGTCGTGTCG TTACCAGCGC TCAGATCTGG GCAGCTAGCT TCTGCAAGAA GAAGGAATTA 
CCTCCCGATT TTATCACCAT CCGTAAGGAC CTTCTTGACG ACTCGAACGC TGCCAAGGAC 
GAGATGGATA AGGTGAAGAA GAAGTTGAAG GGTCTTTTGC GGAAGGGCAA TGAGCGGCCG 
CCCCAGTTTG CCTGGCCTGA AAACATGCCT GAGCCGTCCG AGGTACAGAC ACGCGTGGTC 
CAGCTCATGA ACTTCCACCG CAAGGTCATG CAGCACAACT ACGCCAAGCT TTACAGCGGT 
GCTGTGAACT CTCTCAATGC GATCAACAAC CCTAGTATGG AGATGATCAG TGAGACTTCC 
AGCTCGTCGC TGTCGTCACT TGGCTCATCA CATGTGAACG CGGTGAACAG CATCCAGGCC 
AGATGGTGCT GTGGTGAGGA CGCCGAGCTG TTCAAGGAGC GTTGGGAGAA GCTGTTCGCC 
GAGTTCTGCG ACGGCGAAAA GGTTGACCCT AGCAAGATTT CGGAACTCTA CGATACCATG 
AAGTTCGATG CCCTCCACAA CCGTCAATTC CTCGAATGGG TGTTCACTCC ACCAAAGACT 
ATGCTTGAGG AGGACTACGG AATCCTGGCC CCTCCTAATG GCAGCAAGGA TGCAAAGTCA 
CCGTCCCCTG TTTCGAGCAC CGACAATGAA GCTTCCAAGC CTACCGAGAG CTCATTGGCA 
GAAAAGATCG ACTCAAAGGC TGTCAAGCGT ATCCTCCGCA GGCGGTCGTT CCTACACGGG 
TTTAGACCTG GGCCAGGTCC GGAATCGGAG CTCCCGGAGA GGTACTTCCA CCTCCACAGG 
GGCAACAGCC AGACCAAGGC CAAGACAGAT GCTCGTTTTG AGCCTTTGCG GGAGCTGTAC 
CAGCTTGCTA AGGTCCTCTT TGACTTTATT TGCCCACAAG AGTATGGCAT CTCGGACAGC 
GAAAAGCTTG AAATTGGACT CCTGACCTCT CTCCCTCTGT TGAAGGAGAT TGTCAAGGAC 
CTTGAGGAAA TGCAGGCTTC GGATGAAGCC AAGTCCTTCA TATACTTCAC CAAGGAGTCG 
CACATCTACA CCCTCCTGAA CTGCATCTTG GAGGGCGGCC TAGAGACCAA GATCAAGCGA 
GCTACCATCC CTGAGCTCGA CTACCTCTCG CAGATCTCGT TCGAGCTGTA CGAAATGCCA 
GCTGACCCGC CAACTGATGC GGATGGCATT CCTGCCTTTA ACTACAGCAT CAAGATTACC 
ATTAGCCCTG GCTGTCACGT TTTTGACCCG CTGGATGTAC AGCTTGACAG CAAGCATTGC 
ATTGGCTGTG CTCCGCGCAG GAGCTTAACG GCTCATACAG ACTGGATAAA GGTTATTGAG 
ACGTTGAGGG CGAAGTTCCA CCAAGTAAAA CTGCCCAAGA CTTTCCTGGC GGTTAACCTG 
TCGGATGCCT TTACTTTCCA AGAGAAGCCG CAGGGTGGTG ACGAGGCCAC CGAAGTCGAA 
GCCGAATCAG AGGCGACACC TGAGGCGGAG CGGGTGAAGG AGGTTGAACC GGAGGTGATA 
GAGCCTGATG ACACCGAGGC TGCCGCTGCT AATACCACCG ACGCTGTGAC CGTTGACGAC 
GTAACACCGG ACGAGCACTC TGATGTCCTG GAGATCAAGA TTCCTGAGGA TCAACATGCC 
GTGGACGTTG AAGTTGTTGA CGATGGCAGT GCTGCCGAAC CTGAAGTATC AGAGTCGCCG 
GAAGCTGCAG CGGGAGATGT ATCACTGCTG CCAGAAGTGG AAGCAGCAAC ACCTCCGACT 
GCTGTTGCGT CGAAGACGGA GCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394215.1
CDS	302..5608
Translation	MEAHLEPTSLDRTPVSADSVPSSASEASSSTHPDQDPDTPVATSEAATGGLDVPVEEAEDMAGTPSSSIAVPGAIPITSDSGITSTGKSKRRHSLASSFTNSIASRKSRYSEPGSQSVHADRANVSMPPPVALPQASSSLAKSPRASLSHSRRESSNSFRSFISDAWSDANNSQGYSDLGGGVHVPGSFGSASAVPPLFSPRDFALAAGDNGQNQATIEDSETPRLRPGTGNTNAESPQPPPPQQQQQRQLPDPALQNPTTPTQREYPYHSRRRNSSFFGFEYSQSDSNKRKSISSIYSLASARGVPSSESGSTSGATGPARVVSGLMSSSAPSKSGAIPPASTAQTAEASVSTTTVTTGSQGAPGTHQLSPRESHFQQMGDLLKDGQSRGNHHHTSQAHGHGVPAPVDTAVANGAPPAPPTPRAIPPRSRSRAKRRFSGSTAASGNQSPNGERVVSGHEHRPPKEEHKPAPYGVIGVCALDVKARSKPSRNILNRLIQNGEFDVCVFGDKVILDEEVENWPMCDYLISFYSDGFPLDKAIAYVKARKPFCVNDVPMQKILWDRRLCLRLLDRINVPTPQRIEVNRDGGPHLLTPEICKLIKDVSGVQFEPTNTDPEYAKQVAPRKIELLENGDVLSVDGTLIKKPFVEKPTSGEDHNIIIYFPSYAGGGARKLFRKIGNKSSEYVEDLNVPRCITQPDESFIYEKFMQVDNAEDVKAYTVGPTYCHAETRKSPVVDGVVRRNTHGKEVRYVTALSDEEKEIASKISTAFGQRVCGFDLLRAGGKSYVIDVNGWSFVKDNEDYYNQCAKILREMFIAERQRRGGIPSPCPSPAISEVPDPLAARAALMNKEKELSALAAAQGKVSLDKQRSDNTRHHDAPPHVKSDTSLVSKLSTKPSSPRNSQYGESAPPTAPSTLPTTPSLTSVAIAEQEHEHEETPPPPPPKPSWKLKGVVSVIRHADRTPKQKYKFTFHTDPFIQLLKGHQEEVLLIGEPALASVLDAVDVALKAGIEDPVKLKSLRNVLVKKGGWAGTKVQIKPMFRKKDKPKKEDKEKEGEKPDQEAKEDAAPGEGDSVDKDGNSKPRRPVKRHDSLSGVTMSKFTAAEESLVLDKLQLIVKWGGEPTHSARYQAQELGESMRSDLGLMNRDILEEVHVFSSSERRVVTSAQIWAASFCKKKELPPDFITIRKDLLDDSNAAKDEMDKVKKKLKGLLRKGNERPPQFAWPENMPEPSEVQTRVVQLMNFHRKVMQHNYAKLYSGAVNSLNAINNPSMEMISETSSSSLSSLGSSHVNAVNSIQARWCCGEDAELFKERWEKLFAEFCDGEKVDPSKISELYDTMKFDALHNRQFLEWVFTPPKTMLEEDYGILAPPNGSKDAKSPSPVSSTDNEASKPTESSLAEKIDSKAVKRILRRRSFLHGFRPGPGPESELPERYFHLHRGNSQTKAKTDARFEPLRELYQLAKVLFDFICPQEYGISDSEKLEIGLLTSLPLLKEIVKDLEEMQASDEAKSFIYFTKESHIYTLLNCILEGGLETKIKRATIPELDYLSQISFELYEMPADPPTDADGIPAFNYSIKITISPGCHVFDPLDVQLDSKHCIGCAPRRSLTAHTDWIKVIETLRAKFHQVKLPKTFLAVNLSDAFTFQEKPQGGDEATEVEAESEATPEAERVKEVEPEVIEPDDTEAAAANTTDAVTVDDVTPDEHSDVLEIKIPEDQHAVDVEVVDDGSAAEPEVSESPEAAAGDVSLLPEVEAATPPTAVASKTEQ

Target ORF information:

RefSeq Version	XM_011395913.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A cortical actin cytoskeleton protein asp1 (NCU07010), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395913.1

ORF Insert Sequence:

ATGGAAGCAC ACCTCGAACC CACCTCCCTC GATCGTACGC CAGTGTCCGC CGACAGCGTG 
CCGTCCTCCG CCTCCGAAGC TTCCTCTTCC ACTCATCCGG ACCAAGACCC AGACACTCCT 
GTCGCGACAT CAGAAGCAGC AACCGGCGGC TTGGACGTGC CTGTAGAAGA AGCCGAAGAC 
ATGGCAGGCA CACCGTCGTC GAGCATCGCA GTCCCTGGCG CCATCCCCAT CACCAGTGAC 
AGCGGCATCA CAAGTACCGG AAAATCCAAA CGACGACACA GTCTCGCCTC TTCCTTTACC 
AACAGCATAG CTAGCCGAAA GAGCAGGTAC TCTGAGCCTG GCAGCCAATC TGTTCATGCT 
GATCGCGCCA ATGTGTCGAT GCCCCCTCCC GTCGCCCTGC CACAAGCATC GTCGTCATTA 
GCAAAGAGCC CGCGCGCCTC GCTCTCGCAC AGCCGCCGCG AATCGTCCAA TTCGTTCCGC 
TCCTTCATCT CGGACGCCTG GAGCGACGCA AATAACAGCC AAGGATACTC GGACTTGGGT 
GGTGGAGTAC ATGTGCCCGG CAGCTTTGGC AGCGCCTCGG CGGTCCCACC CCTGTTTTCT 
CCCCGAGATT TCGCCTTGGC CGCCGGCGAT AACGGCCAGA ATCAAGCTAC GATCGAAGAC 
TCGGAGACTC CCCGTCTACG CCCCGGGACC GGTAACACGA ACGCCGAATC ACCTCAACCA 
CCACCACCAC AACAACAACA ACAACGCCAA CTCCCCGATC CCGCTCTGCA GAATCCCACT 
ACTCCCACCC AACGCGAATA CCCCTACCAC TCCCGCCGCA GAAACTCGTC CTTCTTTGGC 
TTCGAGTACA GCCAGTCAGA CAGCAACAAG CGAAAGAGTA TTTCGTCCAT ATACTCTCTA 
GCTTCGGCCC GTGGGGTTCC CAGTTCCGAG AGCGGCAGCA CCAGCGGCGC CACCGGCCCT 
GCCCGAGTGG TATCAGGCTT GATGTCTTCC TCCGCCCCCT CCAAATCGGG AGCCATCCCA 
CCTGCCAGCA CCGCCCAGAC TGCAGAAGCC AGCGTCTCGA CAACCACCGT TACCACGGGA 
AGCCAAGGCG CGCCAGGCAC CCACCAGCTC TCGCCGCGGG AATCGCATTT TCAGCAGATG 
GGCGACTTGC TCAAGGATGG CCAGAGCAGG GGAAACCATC ACCATACTTC GCAGGCCCAC 
GGCCACGGTG TTCCTGCTCC TGTGGACACG GCTGTTGCTA ACGGCGCACC ACCCGCACCA 
CCCACACCGC GCGCAATTCC ACCCAGGTCA AGGAGCAGGG CAAAGCGCAG GTTCAGTGGT 
AGTACCGCTG CTAGCGGAAA TCAGAGTCCC AATGGTGAAA GAGTGGTATC AGGCCATGAA 
CACCGGCCTC CCAAGGAGGA GCACAAGCCT GCGCCCTACG GTGTTATCGG TGTGTGTGCC 
TTGGACGTGA AGGCGAGGAG CAAGCCCAGC AGGAATATCC TCAACCGCTT GATTCAGAAC 
GGCGAATTTG ATGTTTGTGT CTTTGGCGAC AAAGTGATTC TTGATGAAGA GGTCGAAAAC 
TGGCCCATGT GCGACTATTT AATCTCCTTT TACTCGGACG GCTTCCCCCT TGACAAGGCC 
ATCGCCTACG TCAAGGCACG CAAGCCCTTC TGCGTTAACG ATGTCCCTAT GCAAAAGATA 
CTATGGGACC GCCGCCTCTG CCTTCGCCTC TTGGACCGCA TCAATGTCCC CACCCCTCAG 
CGCATCGAAG TGAACCGGGA CGGTGGCCCT CACCTGTTGA CCCCCGAAAT TTGCAAGCTC 
ATCAAGGACG TCAGCGGTGT ACAGTTTGAG CCCACTAATA CGGATCCAGA GTACGCCAAG 
CAGGTCGCGC CGCGCAAGAT CGAGCTCCTC GAAAACGGCG ATGTTCTCTC TGTCGACGGA 
ACGCTCATCA AGAAGCCGTT TGTCGAGAAG CCTACGAGCG GTGAAGACCA CAATATCATC 
ATCTACTTTC CTTCCTACGC TGGCGGTGGT GCAAGGAAGC TTTTCCGCAA GATCGGCAAC 
AAGAGCAGTG AATATGTGGA GGATCTCAAC GTGCCCAGGT GCATCACTCA GCCCGACGAG 
AGTTTCATTT ACGAAAAGTT CATGCAGGTC GACAATGCCG AGGACGTGAA AGCGTATACT 
GTTGGGCCAA CATACTGCCA CGCTGAGACT CGCAAGTCCC CTGTTGTGGA CGGCGTCGTG 
CGAAGAAACA CGCATGGAAA AGAGGTCCGC TATGTCACAG CCTTGAGCGA CGAGGAAAAG 
GAAATTGCGA GCAAAATCAG CACAGCCTTC GGACAACGCG TTTGCGGTTT CGACTTATTG 
CGGGCGGGGG GCAAGAGTTA TGTCATTGAC GTGAACGGCT GGAGTTTCGT CAAAGATAAT 
GAGGACTATT ACAACCAGTG CGCCAAAATC CTCAGGGAAA TGTTTATCGC CGAGAGGCAG 
CGCAGGGGTG GAATACCTTC GCCGTGCCCG TCTCCCGCCA TCTCCGAGGT GCCTGATCCT 
CTTGCCGCTC GCGCAGCTCT CATGAACAAG GAGAAGGAAC TCAGCGCGTT GGCGGCAGCC 
CAGGGTAAGG TGAGCTTGGA CAAGCAGAGG TCGGACAACA CCAGACACCA CGATGCGCCA 
CCGCACGTCA AGTCAGATAC CTCGCTTGTC TCTAAGCTGT CAACCAAGCC GAGCAGCCCG 
CGCAACAGTC AGTACGGAGA GAGTGCACCA CCCACAGCGC CATCAACGCT GCCCACTACC 
CCTTCGCTCA CTTCTGTCGC TATTGCTGAA CAGGAGCATG AGCATGAGGA GACACCGCCG 
CCGCCTCCCC CGAAGCCTAG CTGGAAGCTC AAGGGTGTGG TATCTGTCAT CCGCCACGCT 
GATCGTACGC CGAAGCAGAA GTACAAATTC ACGTTCCACA CAGATCCCTT CATTCAGCTA 
CTCAAGGGTC ACCAAGAAGA AGTGCTCCTG ATTGGTGAGC CGGCGCTGGC AAGTGTACTT 
GATGCTGTTG ATGTGGCCCT GAAGGCTGGT ATTGAAGACC CCGTCAAGCT CAAGTCACTC 
CGCAACGTCC TTGTGAAGAA GGGCGGATGG GCTGGTACCA AGGTCCAAAT AAAGCCAATG 
TTCAGGAAGA AAGACAAGCC CAAGAAGGAA GACAAAGAGA AGGAGGGCGA AAAGCCTGAC 
CAAGAGGCCA AGGAGGATGC TGCACCCGGT GAAGGTGACA GTGTCGACAA GGACGGTAAC 
TCCAAGCCTC GTAGGCCAGT CAAGAGGCAC GATTCCTTGT CAGGCGTTAC CATGTCCAAG 
TTCACTGCTG CCGAGGAGAG CTTGGTGCTG GATAAGCTTC AGCTCATTGT GAAATGGGGC 
GGCGAGCCGA CACACTCCGC CCGCTACCAG GCACAGGAAC TCGGTGAGAG CATGCGTAGT 
GATCTTGGTC TCATGAACCG TGATATCCTG GAGGAGGTCC ACGTCTTCAG CAGCAGCGAG 
CGTCGTGTCG TTACCAGCGC TCAGATCTGG GCAGCTAGCT TCTGCAAGAA GAAGGAATTA 
CCTCCCGATT TTATCACCAT CCGTAAGGAC CTTCTTGACG ACTCGAACGC TGCCAAGGAC 
GAGATGGATA AGGTGAAGAA GAAGTTGAAG GGTCTTTTGC GGAAGGGCAA TGAGCGGCCG 
CCCCAGTTTG CCTGGCCTGA AAACATGCCT GAGCCGTCCG AGGTACAGAC ACGCGTGGTC 
CAGCTCATGA ACTTCCACCG CAAGGTCATG CAGCACAACT ACGCCAAGCT TTACAGCGGT 
GCTGTGAACT CTCTCAATGC GATCAACAAC CCTAGTATGG AGATGATCAG TGAGACTTCC 
AGCTCGTCGC TGTCGTCACT TGGCTCATCA CATGTGAACG CGGTGAACAG CATCCAGGCC 
AGATGGTGCT GTGGTGAGGA CGCCGAGCTG TTCAAGGAGC GTTGGGAGAA GCTGTTCGCC 
GAGTTCTGCG ACGGCGAAAA GGTTGACCCT AGCAAGATTT CGGAACTCTA CGATACCATG 
AAGTTCGATG CCCTCCACAA CCGTCAATTC CTCGAATGGG TGTTCACTCC ACCAAAGACT 
ATGCTTGAGG AGGACTACGG AATCCTGGCC CCTCCTAATG GCAGCAAGGA TGCAAAGTCA 
CCGTCCCCTG TTTCGAGCAC CGACAATGAA GCTTCCAAGC CTACCGAGAG CTCATTGGCA 
GAAAAGATCG ACTCAAAGGC TGTCAAGCGT ATCCTCCGCA GGCGGTCGTT CCTACACGGG 
TTTAGACCTG GGCCAGGTCC GGAATCGGAG CTCCCGGAGA GGTACTTCCA CCTCCACAGG 
GGCAACAGCC AGACCAAGGC CAAGACAGAT GCTCGTTTTG AGCCTTTGCG GGAGCTGTAC 
CAGCTTGCTA AGGTCCTCTT TGACTTTATT TGCCCACAAG AGTATGGCAT CTCGGACAGC 
GAAAAGCTTG AAATTGGACT CCTGACCTCT CTCCCTCTGT TGAAGGAGAT TGTCAAGGAC 
CTTGAGGAAA TGCAGGCTTC GGATGAAGCC AAGTCCTTCA TATACTTCAC CAAGGAGTCG 
CACATCTACA CCCTCCTGAA CTGCATCTTG GAGGGCGGCC TAGAGACCAA GATCAAGCGA 
GCTACCATCC CTGAGCTCGA CTACCTCTCG CAGATCTCGT TCGAGCTGTA CGAAATGCCA 
GCTGACCCGC CAACTGATGC GGATGGCATT CCTGCCTTTA ACTACAGCAT CAAGATTACC 
ATTAGCCCTG GCTGTCACGT TTTTGACCCG CTGGATGTAC AGCTTGACAG CAAGCATTGC 
ATTGGCTGTG CTCCGCGCAG GAGCTTAACG GCTCATACAG ACTGGATAAA GGTTATTGAG 
ACGTTGAGGG CGAAGTTCCA CCAAGTAAAA CTGCCCAAGA CTTTCCTGGC GGTTAACCTG 
TCGGATGCCT TTACTTTCCA AGAGAAGCCG CAGGGTGGTG ACGAGGCCAC CGAAGTCGAA 
GCCGAATCAG AGGCGACACC TGAGGCGGAG CGGGTGAAGG AGGTTGAACC GGAGGTGATA 
GAGCCTGATG ACACCGAGGC TGCCGCTGCT AATACCACCG ACGCTGTGAC CGTTGACGAC 
GTAACACCGG ACGAGCACTC TGATGTCCTG GAGATCAAGA TTCCTGAGGA TCAACATGCC 
GTGGACGTTG AAGTTGTTGA CGATGGCAGT GCTGCCGAAC CTGAAGTATC AGAGTCGCCG 
GAAGCTGCAG CGGGAGATGT ATCACTGCTG CCAGAAGTGG AAGCAGCAAC ACCTCCGACT 
GCTGTTGCGT CGAAGACGGA GCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU07010 ( XM_011395914.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394216.1 Neurospora crassa OR74A cortical actin cytoskeleton protein asp1, variant (NCU07010), mRNA.

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CloneID	ONf11891
Clone ID Related Accession (Same CDS sequence)	XM_011395914.1
Accession Version	XM_011395914.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	cortical actin cytoskeleton protein asp1, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504).

ATGTGCCCGG CAGCTTTGGC AGCGCCTCGG CGGTCCCACC CCTGTTTTCT CCCCGAGATT 
TCGCCTTGGC CGCCGGCGAT AACGGCCAGA ATCAAGGTCA GAGTCATAAT CATTCTTGCT 
CCACTACCGT CATCACTAAT TCCGTCCGGT TCAGCTACGA TCGAAGACTC GGAGACTCCC 
CGTCTACGCC CCGGGACCGG TAACACGAAC GCCGAATCAC CTCAACCACC ACCACCACAA 
CAACAACAAC AACGCCAACT CCCCGATCCC GCTCTGCAGA ATCCCACTAC TCCCACCCAA 
CGCGAATACC CCTACCACTC CCGCCGCAGA AACTCGTCCT TCTTTGGCTT CGAGTACAGC 
CAGTCAGACA GCAACAAGCG AAAGAGTATT TCGTCCATAT ACTCTCTAGC TTCGGCCCGT 
GGGGTTCCCA GTTCCGAGAG CGGCAGCACC AGCGGCGCCA CCGGCCCTGC CCGAGTGGTA 
TCAGGCTTGA TGTCTTCCTC CGCCCCCTCC AAATCGGGAG CCATCCCACC TGCCAGCACC 
GCCCAGACTG CAGAAGCCAG CGTCTCGACA ACCACCGTTA CCACGGGAAG CCAAGGCGCG 
CCAGGCACCC ACCAGCTCTC GCCGCGGGAA TCGCATTTTC AGCAGATGGG CGACTTGCTC 
AAGGATGGCC AGAGCAGGGG AAACCATCAC CATACTTCGC AGGCCCACGG CCACGGTGTT 
CCTGCTCCTG TGGACACGGC TGTTGCTAAC GGCGCACCAC CCGCACCACC CACACCGCGC 
GCAATTCCAC CCAGGTCAAG GAGCAGGGCA AAGCGCAGGT TCAGTGGTAG TACCGCTGCT 
AGCGGAAATC AGAGTCCCAA TGGTGAAAGA GTGGTATCAG GCCATGAACA CCGGCCTCCC 
AAGGAGGAGC ACAAGCCTGC GCCCTACGGT GTTATCGGTG TGTGTGCCTT GGACGTGAAG 
GCGAGGAGCA AGCCCAGCAG GAATATCCTC AACCGCTTGA TTCAGAACGG CGAATTTGAT 
GTTTGTGTCT TTGGCGACAA AGTGATTCTT GATGAAGAGG TCGAAAACTG GCCCATGTGC 
GACTATTTAA TCTCCTTTTA CTCGGACGGC TTCCCCCTTG ACAAGGCCAT CGCCTACGTC 
AAGGCACGCA AGCCCTTCTG CGTTAACGAT GTCCCTATGC AAAAGATACT ATGGGACCGC 
CGCCTCTGCC TTCGCCTCTT GGACCGCATC AATGTCCCCA CCCCTCAGCG CATCGAAGTG 
AACCGGGACG GTGGCCCTCA CCTGTTGACC CCCGAAATTT GCAAGCTCAT CAAGGACGTC 
AGCGGTGTAC AGTTTGAGCC CACTAATACG GATCCAGAGT ACGCCAAGCA GGTCGCGCCG 
CGCAAGATCG AGCTCCTCGA AAACGGCGAT GTTCTCTCTG TCGACGGAAC GCTCATCAAG 
AAGCCGTTTG TCGAGAAGCC TACGAGCGGT GAAGACCACA ATATCATCAT CTACTTTCCT 
TCCTACGCTG GCGGTGGTGC AAGGAAGCTT TTCCGCAAGA TCGGCAACAA GAGCAGTGAA 
TATGTGGAGG ATCTCAACGT GCCCAGGTGC ATCACTCAGC CCGACGAGAG TTTCATTTAC 
GAAAAGTTCA TGCAGGTCGA CAATGCCGAG GACGTGAAAG CGTATACTGT TGGGCCAACA 
TACTGCCACG CTGAGACTCG CAAGTCCCCT GTTGTGGACG GCGTCGTGCG AAGAAACACG 
CATGGAAAAG AGGTCCGCTA TGTCACAGCC TTGAGCGACG AGGAAAAGGA AATTGCGAGC 
AAAATCAGCA CAGCCTTCGG ACAACGCGTT TGCGGTTTCG ACTTATTGCG GGCGGGGGGC 
AAGAGTTATG TCATTGACGT GAACGGCTGG AGTTTCGTCA AAGATAATGA GGACTATTAC 
AACCAGTGCG CCAAAATCCT CAGGGAAATG TTTATCGCCG AGAGGCAGCG CAGGGGTGGA 
ATACCTTCGC CGTGCCCGTC TCCCGCCATC TCCGAGGTGC CTGATCCTCT TGCCGCTCGC 
GCAGCTCTCA TGAACAAGGA GAAGGAACTC AGCGCGTTGG CGGCAGCCCA GGGTAAGGTG 
AGCTTGGACA AGCAGAGGTC GGACAACACC AGACACCACG ATGCGCCACC GCACGTCAAG 
TCAGATACCT CGCTTGTCTC TAAGCTGTCA ACCAAGCCGA GCAGCCCGCG CAACAGTCAG 
TACGGAGAGA GTGCACCACC CACAGCGCCA TCAACGCTGC CCACTACCCC TTCGCTCACT 
TCTGTCGCTA TTGCTGAACA GGAGCATGAG CATGAGGAGA CACCGCCGCC GCCTCCCCCG 
AAGCCTAGCT GGAAGCTCAA GGGTGTGGTA TCTGTCATCC GCCACGCTGA TCGTACGCCG 
AAGCAGAAGT ACAAATTCAC GTTCCACACA GATCCCTTCA TTCAGCTACT CAAGGGTCAC 
CAAGAAGAAG TGCTCCTGAT TGGTGAGCCG GCGCTGGCAA GTGTACTTGA TGCTGTTGAT 
GTGGCCCTGA AGGCTGGTAT TGAAGACCCC GTCAAGCTCA AGTCACTCCG CAACGTCCTT 
GTGAAGAAGG GCGGATGGGC TGGTACCAAG GTCCAAATAA AGCCAATGTT CAGGAAGAAA 
GACAAGCCCA AGAAGGAAGA CAAAGAGAAG GAGGGCGAAA AGCCTGACCA AGAGGCCAAG 
GAGGATGCTG CACCCGGTGA AGGTGACAGT GTCGACAAGG ACGGTAACTC CAAGCCTCGT 
AGGCCAGTCA AGAGGCACGA TTCCTTGTCA GGCGTTACCA TGTCCAAGTT CACTGCTGCC 
GAGGAGAGCT TGGTGCTGGA TAAGCTTCAG CTCATTGTGA AATGGGGCGG CGAGCCGACA 
CACTCCGCCC GCTACCAGGC ACAGGAACTC GGTGAGAGCA TGCGTAGTGA TCTTGGTCTC 
ATGAACCGTG ATATCCTGGA GGAGGTCCAC GTCTTCAGCA GCAGCGAGCG TCGTGTCGTT 
ACCAGCGCTC AGATCTGGGC AGCTAGCTTC TGCAAGAAGA AGGAATTACC TCCCGATTTT 
ATCACCATCC GTAAGGACCT TCTTGACGAC TCGAACGCTG CCAAGGACGA GATGGATAAG 
GTGAAGAAGA AGTTGAAGGG TCTTTTGCGG AAGGGCAATG AGCGGCCGCC CCAGTTTGCC 
TGGCCTGAAA ACATGCCTGA GCCGTCCGAG GTACAGACAC GCGTGGTCCA GCTCATGAAC 
TTCCACCGCA AGGTCATGCA GCACAACTAC GCCAAGCTTT ACAGCGGTGC TGTGAACTCT 
CTCAATGCGA TCAACAACCC TAGTATGGAG ATGATCAGTG AGACTTCCAG CTCGTCGCTG 
TCGTCACTTG GCTCATCACA TGTGAACGCG GTGAACAGCA TCCAGGCCAG ATGGTGCTGT 
GGTGAGGACG CCGAGCTGTT CAAGGAGCGT TGGGAGAAGC TGTTCGCCGA GTTCTGCGAC 
GGCGAAAAGG TTGACCCTAG CAAGATTTCG GAACTCTACG ATACCATGAA GTTCGATGCC 
CTCCACAACC GTCAATTCCT CGAATGGGTG TTCACTCCAC CAAAGACTAT GCTTGAGGAG 
GACTACGGAA TCCTGGCCCC TCCTAATGGC AGCAAGGATG CAAAGTCACC GTCCCCTGTT 
TCGAGCACCG ACAATGAAGC TTCCAAGCCT ACCGAGAGCT CATTGGCAGA AAAGATCGAC 
TCAAAGGCTG TCAAGCGTAT CCTCCGCAGG CGGTCGTTCC TACACGGGTT TAGACCTGGG 
CCAGGTCCGG AATCGGAGCT CCCGGAGAGG TACTTCCACC TCCACAGGGG CAACAGCCAG 
ACCAAGGCCA AGACAGATGC TCGTTTTGAG CCTTTGCGGG AGCTGTACCA GCTTGCTAAG 
GTCCTCTTTG ACTTTATTTG CCCACAAGAG TATGGCATCT CGGACAGCGA AAAGCTTGAA 
ATTGGACTCC TGACCTCTCT CCCTCTGTTG AAGGAGATTG TCAAGGACCT TGAGGAAATG 
CAGGCTTCGG ATGAAGCCAA GTCCTTCATA TACTTCACCA AGGAGTCGCA CATCTACACC 
CTCCTGAACT GCATCTTGGA GGGCGGCCTA GAGACCAAGA TCAAGCGAGC TACCATCCCT 
GAGCTCGACT ACCTCTCGCA GATCTCGTTC GAGCTGTACG AAATGCCAGC TGACCCGCCA 
ACTGATGCGG ATGGCATTCC TGCCTTTAAC TACAGCATCA AGATTACCAT TAGCCCTGGC 
TGTCACGTTT TTGACCCGCT GGATGTACAG CTTGACAGCA AGCATTGCAT TGGCTGTGCT 
CCGCGCAGGA GCTTAACGGC TCATACAGAC TGGATAAAGG TTATTGAGAC GTTGAGGGCG 
AAGTTCCACC AAGTAAAACT GCCCAAGACT TTCCTGGCGG TTAACCTGTC GGATGCCTTT 
ACTTTCCAAG AGAAGCCGCA GGGTGGTGAC GAGGCCACCG AAGTCGAAGC CGAATCAGAG 
GCGACACCTG AGGCGGAGCG GGTGAAGGAG GTTGAACCGG AGGTGATAGA GCCTGATGAC 
ACCGAGGCTG CCGCTGCTAA TACCACCGAC GCTGTGACCG TTGACGACGT AACACCGGAC 
GAGCACTCTG ATGTCCTGGA GATCAAGATT CCTGAGGATC AACATGCCGT GGACGTTGAA 
GTTGTTGACG ATGGCAGTGC TGCCGAACCT GAAGTATCAG AGTCGCCGGA AGCTGCAGCG 
GGAGATGTAT CACTGCTGCC AGAAGTGGAA GCAGCAACAC CTCCGACTGC TGTTGCGTCG 
AAGACGGAGC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394216.1
CDS	852..5666
Translation	MCPAALAAPRRSHPCFLPEISPWPPAITARIKVRVIIILAPLPSSLIPSGSATIEDSETPRLRPGTGNTNAESPQPPPPQQQQQRQLPDPALQNPTTPTQREYPYHSRRRNSSFFGFEYSQSDSNKRKSISSIYSLASARGVPSSESGSTSGATGPARVVSGLMSSSAPSKSGAIPPASTAQTAEASVSTTTVTTGSQGAPGTHQLSPRESHFQQMGDLLKDGQSRGNHHHTSQAHGHGVPAPVDTAVANGAPPAPPTPRAIPPRSRSRAKRRFSGSTAASGNQSPNGERVVSGHEHRPPKEEHKPAPYGVIGVCALDVKARSKPSRNILNRLIQNGEFDVCVFGDKVILDEEVENWPMCDYLISFYSDGFPLDKAIAYVKARKPFCVNDVPMQKILWDRRLCLRLLDRINVPTPQRIEVNRDGGPHLLTPEICKLIKDVSGVQFEPTNTDPEYAKQVAPRKIELLENGDVLSVDGTLIKKPFVEKPTSGEDHNIIIYFPSYAGGGARKLFRKIGNKSSEYVEDLNVPRCITQPDESFIYEKFMQVDNAEDVKAYTVGPTYCHAETRKSPVVDGVVRRNTHGKEVRYVTALSDEEKEIASKISTAFGQRVCGFDLLRAGGKSYVIDVNGWSFVKDNEDYYNQCAKILREMFIAERQRRGGIPSPCPSPAISEVPDPLAARAALMNKEKELSALAAAQGKVSLDKQRSDNTRHHDAPPHVKSDTSLVSKLSTKPSSPRNSQYGESAPPTAPSTLPTTPSLTSVAIAEQEHEHEETPPPPPPKPSWKLKGVVSVIRHADRTPKQKYKFTFHTDPFIQLLKGHQEEVLLIGEPALASVLDAVDVALKAGIEDPVKLKSLRNVLVKKGGWAGTKVQIKPMFRKKDKPKKEDKEKEGEKPDQEAKEDAAPGEGDSVDKDGNSKPRRPVKRHDSLSGVTMSKFTAAEESLVLDKLQLIVKWGGEPTHSARYQAQELGESMRSDLGLMNRDILEEVHVFSSSERRVVTSAQIWAASFCKKKELPPDFITIRKDLLDDSNAAKDEMDKVKKKLKGLLRKGNERPPQFAWPENMPEPSEVQTRVVQLMNFHRKVMQHNYAKLYSGAVNSLNAINNPSMEMISETSSSSLSSLGSSHVNAVNSIQARWCCGEDAELFKERWEKLFAEFCDGEKVDPSKISELYDTMKFDALHNRQFLEWVFTPPKTMLEEDYGILAPPNGSKDAKSPSPVSSTDNEASKPTESSLAEKIDSKAVKRILRRRSFLHGFRPGPGPESELPERYFHLHRGNSQTKAKTDARFEPLRELYQLAKVLFDFICPQEYGISDSEKLEIGLLTSLPLLKEIVKDLEEMQASDEAKSFIYFTKESHIYTLLNCILEGGLETKIKRATIPELDYLSQISFELYEMPADPPTDADGIPAFNYSIKITISPGCHVFDPLDVQLDSKHCIGCAPRRSLTAHTDWIKVIETLRAKFHQVKLPKTFLAVNLSDAFTFQEKPQGGDEATEVEAESEATPEAERVKEVEPEVIEPDDTEAAAANTTDAVTVDDVTPDEHSDVLEIKIPEDQHAVDVEVVDDGSAAEPEVSESPEAAAGDVSLLPEVEAATPPTAVASKTEQ

Target ORF information:

RefSeq Version	XM_011395914.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A cortical actin cytoskeleton protein asp1, variant (NCU07010), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395914.1

ORF Insert Sequence:

ATGTGCCCGG CAGCTTTGGC AGCGCCTCGG CGGTCCCACC CCTGTTTTCT CCCCGAGATT 
TCGCCTTGGC CGCCGGCGAT AACGGCCAGA ATCAAGGTCA GAGTCATAAT CATTCTTGCT 
CCACTACCGT CATCACTAAT TCCGTCCGGT TCAGCTACGA TCGAAGACTC GGAGACTCCC 
CGTCTACGCC CCGGGACCGG TAACACGAAC GCCGAATCAC CTCAACCACC ACCACCACAA 
CAACAACAAC AACGCCAACT CCCCGATCCC GCTCTGCAGA ATCCCACTAC TCCCACCCAA 
CGCGAATACC CCTACCACTC CCGCCGCAGA AACTCGTCCT TCTTTGGCTT CGAGTACAGC 
CAGTCAGACA GCAACAAGCG AAAGAGTATT TCGTCCATAT ACTCTCTAGC TTCGGCCCGT 
GGGGTTCCCA GTTCCGAGAG CGGCAGCACC AGCGGCGCCA CCGGCCCTGC CCGAGTGGTA 
TCAGGCTTGA TGTCTTCCTC CGCCCCCTCC AAATCGGGAG CCATCCCACC TGCCAGCACC 
GCCCAGACTG CAGAAGCCAG CGTCTCGACA ACCACCGTTA CCACGGGAAG CCAAGGCGCG 
CCAGGCACCC ACCAGCTCTC GCCGCGGGAA TCGCATTTTC AGCAGATGGG CGACTTGCTC 
AAGGATGGCC AGAGCAGGGG AAACCATCAC CATACTTCGC AGGCCCACGG CCACGGTGTT 
CCTGCTCCTG TGGACACGGC TGTTGCTAAC GGCGCACCAC CCGCACCACC CACACCGCGC 
GCAATTCCAC CCAGGTCAAG GAGCAGGGCA AAGCGCAGGT TCAGTGGTAG TACCGCTGCT 
AGCGGAAATC AGAGTCCCAA TGGTGAAAGA GTGGTATCAG GCCATGAACA CCGGCCTCCC 
AAGGAGGAGC ACAAGCCTGC GCCCTACGGT GTTATCGGTG TGTGTGCCTT GGACGTGAAG 
GCGAGGAGCA AGCCCAGCAG GAATATCCTC AACCGCTTGA TTCAGAACGG CGAATTTGAT 
GTTTGTGTCT TTGGCGACAA AGTGATTCTT GATGAAGAGG TCGAAAACTG GCCCATGTGC 
GACTATTTAA TCTCCTTTTA CTCGGACGGC TTCCCCCTTG ACAAGGCCAT CGCCTACGTC 
AAGGCACGCA AGCCCTTCTG CGTTAACGAT GTCCCTATGC AAAAGATACT ATGGGACCGC 
CGCCTCTGCC TTCGCCTCTT GGACCGCATC AATGTCCCCA CCCCTCAGCG CATCGAAGTG 
AACCGGGACG GTGGCCCTCA CCTGTTGACC CCCGAAATTT GCAAGCTCAT CAAGGACGTC 
AGCGGTGTAC AGTTTGAGCC CACTAATACG GATCCAGAGT ACGCCAAGCA GGTCGCGCCG 
CGCAAGATCG AGCTCCTCGA AAACGGCGAT GTTCTCTCTG TCGACGGAAC GCTCATCAAG 
AAGCCGTTTG TCGAGAAGCC TACGAGCGGT GAAGACCACA ATATCATCAT CTACTTTCCT 
TCCTACGCTG GCGGTGGTGC AAGGAAGCTT TTCCGCAAGA TCGGCAACAA GAGCAGTGAA 
TATGTGGAGG ATCTCAACGT GCCCAGGTGC ATCACTCAGC CCGACGAGAG TTTCATTTAC 
GAAAAGTTCA TGCAGGTCGA CAATGCCGAG GACGTGAAAG CGTATACTGT TGGGCCAACA 
TACTGCCACG CTGAGACTCG CAAGTCCCCT GTTGTGGACG GCGTCGTGCG AAGAAACACG 
CATGGAAAAG AGGTCCGCTA TGTCACAGCC TTGAGCGACG AGGAAAAGGA AATTGCGAGC 
AAAATCAGCA CAGCCTTCGG ACAACGCGTT TGCGGTTTCG ACTTATTGCG GGCGGGGGGC 
AAGAGTTATG TCATTGACGT GAACGGCTGG AGTTTCGTCA AAGATAATGA GGACTATTAC 
AACCAGTGCG CCAAAATCCT CAGGGAAATG TTTATCGCCG AGAGGCAGCG CAGGGGTGGA 
ATACCTTCGC CGTGCCCGTC TCCCGCCATC TCCGAGGTGC CTGATCCTCT TGCCGCTCGC 
GCAGCTCTCA TGAACAAGGA GAAGGAACTC AGCGCGTTGG CGGCAGCCCA GGGTAAGGTG 
AGCTTGGACA AGCAGAGGTC GGACAACACC AGACACCACG ATGCGCCACC GCACGTCAAG 
TCAGATACCT CGCTTGTCTC TAAGCTGTCA ACCAAGCCGA GCAGCCCGCG CAACAGTCAG 
TACGGAGAGA GTGCACCACC CACAGCGCCA TCAACGCTGC CCACTACCCC TTCGCTCACT 
TCTGTCGCTA TTGCTGAACA GGAGCATGAG CATGAGGAGA CACCGCCGCC GCCTCCCCCG 
AAGCCTAGCT GGAAGCTCAA GGGTGTGGTA TCTGTCATCC GCCACGCTGA TCGTACGCCG 
AAGCAGAAGT ACAAATTCAC GTTCCACACA GATCCCTTCA TTCAGCTACT CAAGGGTCAC 
CAAGAAGAAG TGCTCCTGAT TGGTGAGCCG GCGCTGGCAA GTGTACTTGA TGCTGTTGAT 
GTGGCCCTGA AGGCTGGTAT TGAAGACCCC GTCAAGCTCA AGTCACTCCG CAACGTCCTT 
GTGAAGAAGG GCGGATGGGC TGGTACCAAG GTCCAAATAA AGCCAATGTT CAGGAAGAAA 
GACAAGCCCA AGAAGGAAGA CAAAGAGAAG GAGGGCGAAA AGCCTGACCA AGAGGCCAAG 
GAGGATGCTG CACCCGGTGA AGGTGACAGT GTCGACAAGG ACGGTAACTC CAAGCCTCGT 
AGGCCAGTCA AGAGGCACGA TTCCTTGTCA GGCGTTACCA TGTCCAAGTT CACTGCTGCC 
GAGGAGAGCT TGGTGCTGGA TAAGCTTCAG CTCATTGTGA AATGGGGCGG CGAGCCGACA 
CACTCCGCCC GCTACCAGGC ACAGGAACTC GGTGAGAGCA TGCGTAGTGA TCTTGGTCTC 
ATGAACCGTG ATATCCTGGA GGAGGTCCAC GTCTTCAGCA GCAGCGAGCG TCGTGTCGTT 
ACCAGCGCTC AGATCTGGGC AGCTAGCTTC TGCAAGAAGA AGGAATTACC TCCCGATTTT 
ATCACCATCC GTAAGGACCT TCTTGACGAC TCGAACGCTG CCAAGGACGA GATGGATAAG 
GTGAAGAAGA AGTTGAAGGG TCTTTTGCGG AAGGGCAATG AGCGGCCGCC CCAGTTTGCC 
TGGCCTGAAA ACATGCCTGA GCCGTCCGAG GTACAGACAC GCGTGGTCCA GCTCATGAAC 
TTCCACCGCA AGGTCATGCA GCACAACTAC GCCAAGCTTT ACAGCGGTGC TGTGAACTCT 
CTCAATGCGA TCAACAACCC TAGTATGGAG ATGATCAGTG AGACTTCCAG CTCGTCGCTG 
TCGTCACTTG GCTCATCACA TGTGAACGCG GTGAACAGCA TCCAGGCCAG ATGGTGCTGT 
GGTGAGGACG CCGAGCTGTT CAAGGAGCGT TGGGAGAAGC TGTTCGCCGA GTTCTGCGAC 
GGCGAAAAGG TTGACCCTAG CAAGATTTCG GAACTCTACG ATACCATGAA GTTCGATGCC 
CTCCACAACC GTCAATTCCT CGAATGGGTG TTCACTCCAC CAAAGACTAT GCTTGAGGAG 
GACTACGGAA TCCTGGCCCC TCCTAATGGC AGCAAGGATG CAAAGTCACC GTCCCCTGTT 
TCGAGCACCG ACAATGAAGC TTCCAAGCCT ACCGAGAGCT CATTGGCAGA AAAGATCGAC 
TCAAAGGCTG TCAAGCGTAT CCTCCGCAGG CGGTCGTTCC TACACGGGTT TAGACCTGGG 
CCAGGTCCGG AATCGGAGCT CCCGGAGAGG TACTTCCACC TCCACAGGGG CAACAGCCAG 
ACCAAGGCCA AGACAGATGC TCGTTTTGAG CCTTTGCGGG AGCTGTACCA GCTTGCTAAG 
GTCCTCTTTG ACTTTATTTG CCCACAAGAG TATGGCATCT CGGACAGCGA AAAGCTTGAA 
ATTGGACTCC TGACCTCTCT CCCTCTGTTG AAGGAGATTG TCAAGGACCT TGAGGAAATG 
CAGGCTTCGG ATGAAGCCAA GTCCTTCATA TACTTCACCA AGGAGTCGCA CATCTACACC 
CTCCTGAACT GCATCTTGGA GGGCGGCCTA GAGACCAAGA TCAAGCGAGC TACCATCCCT 
GAGCTCGACT ACCTCTCGCA GATCTCGTTC GAGCTGTACG AAATGCCAGC TGACCCGCCA 
ACTGATGCGG ATGGCATTCC TGCCTTTAAC TACAGCATCA AGATTACCAT TAGCCCTGGC 
TGTCACGTTT TTGACCCGCT GGATGTACAG CTTGACAGCA AGCATTGCAT TGGCTGTGCT 
CCGCGCAGGA GCTTAACGGC TCATACAGAC TGGATAAAGG TTATTGAGAC GTTGAGGGCG 
AAGTTCCACC AAGTAAAACT GCCCAAGACT TTCCTGGCGG TTAACCTGTC GGATGCCTTT 
ACTTTCCAAG AGAAGCCGCA GGGTGGTGAC GAGGCCACCG AAGTCGAAGC CGAATCAGAG 
GCGACACCTG AGGCGGAGCG GGTGAAGGAG GTTGAACCGG AGGTGATAGA GCCTGATGAC 
ACCGAGGCTG CCGCTGCTAA TACCACCGAC GCTGTGACCG TTGACGACGT AACACCGGAC 
GAGCACTCTG ATGTCCTGGA GATCAAGATT CCTGAGGATC AACATGCCGT GGACGTTGAA 
GTTGTTGACG ATGGCAGTGC TGCCGAACCT GAAGTATCAG AGTCGCCGGA AGCTGCAGCG 
GGAGATGTAT CACTGCTGCC AGAAGTGGAA GCAGCAACAC CTCCGACTGC TGTTGCGTCG 
AAGACGGAGC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU07010 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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