wc-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	wc-1
Entrez Gene ID	3875924
Full Name	white collar-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011396849.1	XP_011395151.1	white collar 1 protein
XM_011396851.1	XP_011395153.1	white collar 1 protein, variant 3
XM_011396850.1	XP_011395152.1	white collar 1 protein, variant 1
XM_011396848.1	XP_011395150.1	white collar 1 protein, variant 2

Pathways from BioSystems

Homologies

Neurospora crassa	NCU02356	XP_959777.2
Magnaporthe oryzae (rice blast fungus)	MGG_03538	XP_003716379.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DNA binding for light induction by the White Collar complex requires only WC-2, whereas DNA binding for circadian functions requires WC-2 .
Title: Alternative Use of DNA Binding Domains by the Neurospora White Collar Complex Dictates Circadian Regulation and Light Responses.

WC-1 stability is reduced in the fastest photocycle mutant even in the presence of clock gene frequency as a second stabilizing factor.
Title: Biological Significance of Photoreceptor Photocycle Length: VIVID Photocycle Governs the Dynamic VIVID-White Collar Complex Pool Mediating Photo-adaptation and Response to Changes in Light Intensity.

Our data suggest that WCC acts as a general switch for light-induced chromatin remodeling and gene expression. SUB1 and FF7 synergistically determine the extent of light-induction of target genes in common with WCC( WC1 and WC2)
Title: Combinatorial control of light induced chromatin remodeling and gene activation in Neurospora.

The not1 transcript displays a circadian oscillation with a similar phase as frq. Down-regulation of not1 leads to low levels of WC-1 and WC-2 and a delayed circadian phase as a result of increased protein degradation and increased WC activity.
Title: The Ccr4-not protein complex regulates the phase of the Neurospora circadian clock by controlling white collar protein stability and activity.

A WC-1/NGF-1 complex is preassembled in the dark on light-inducible promoters that, after exposure to light stimulation, leads to histone H3 acetylation and transcriptional activation.
Title: Physical association of the WC-1 photoreceptor and the histone acetyltransferase NGF-1 is required for blue light signal transduction in Neurospora crassa.

The following wc-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wc-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONf11183	XM_011396851.1 Latest version!	Neurospora crassa OR74A white collar 1 protein, variant 3 (wc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONf11183	XM_011396848.1 Latest version!	Neurospora crassa OR74A white collar 1 protein, variant 2 (wc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONf11183	XM_011396850.1 Latest version!	Neurospora crassa OR74A white collar 1 protein, variant 1 (wc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ONf11183	XM_011396849.1 Latest version!	Neurospora crassa OR74A white collar 1 protein (wc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wc-1 ( XM_011396849.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011395151.1 Neurospora crassa OR74A white collar 1 protein (wc-1), mRNA.

Price & Availability↑

CloneID	ONf11183
Clone ID Related Accession (Same CDS sequence)	XM_011396849.1 , XM_011396851.1 , XM_011396850.1 , XM_011396848.1
Accession Version	XM_011396849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	white collar 1 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507).

ATGAACAACA ACTACTACGG TTCCCCGCTG TCTCCTGAGG AGCTTCAGCA TCAAATGCAC 
CAGCACCAGC AGCAGCAGCA GCAACAACAA CAACAACAAC AGCAGCAGCA GCAGCAGCAA 
CAACAACAAC AGCAACAACA GCAACAACAG CATCAGCATC AGCAGCAACA AAAAACCAAT 
CAGCATCGCA ATGCCGGCAT GATGAATACG CCTCCAACTA CAAATCAAGG AAACAGCACG 
ATTCACGCTT CAGATGTAAC CATGTCAGGA GGAAGCGACT CTCTTGATGA AATCATCCAG 
CAGAACCTCG ACGAAATGCA TCGAAGGCGG AGTGTCCCCC AGCCCTATGG GGGCCAGACT 
AGGAGGTTGT CCATGTTTGA TTACGCGAAC CCCAACGATG GTTTCTCGGA CTATCAACTC 
GATAATATGT CAGGAAATTA CGGGGACATG ACTGGCGGCA TGGGCATGTC AGGGCACTCG 
AGCCCGTATG CAGGGCAGAA CATCATGGCC ATGTCGGACC ACAGCGGTGG ATACTCGCAC 
ATGTCGCCCA ATGTTATGGG CAACATGATG ACTTACCCCA ACCTCAACAT GTACCACTCT 
CCCCCGATCG AGAACCCCTA TTCTTCGGCC GGACTCGACA CTATTCGCAC CGATTTTTCC 
ATGGACATGA ACATGGATAG TGGCTCCGTC AGCGCAGCCA GCGTACATCC CACCCCTGGG 
CTTAATAAGC AGGACGACGA GATGATGACC ATGGAGCAGG GCTTTGGTGG AGGTGACGAT 
GCCAACGCCT CTCACCAGGC CCAACAAAAC ATGGGCGGTT TGACTCCGGC CATGACTCCG 
GCCATGACCC CTGCAATGAC CCCGGGTGTC AGCAACTTTG CACAAGGCAT GGCTACACCA 
GTCTCACAGG ATGCGGCAAG CACACCCGCG ACAACCTTCC AGTCTCCGTC CTTATCCGCA 
ACAACACAGA CAATACGAAT TGGGCCGCCG CCACCGCCGA GCGTCACCAA TGCCCCCACC 
CCAGCGCCAT TTACCTCGAC GCCGTCAGGT GGAGGAGCGT CCCAGACAAA GAGCATTTAC 
TCCAAAAGCG GTTTCGACAT GCTGAGGGCG TTGTGGTATG TGGCATCGAG GAAGGACCCA 
AAGCTTAAGC TCGGTGCCGT GGACATGTCT TGTGCATTTG TCGTCTGTGA TGTAACACTC 
AACGATTGCC CCATCATTTA TGTGTCCGAT AACTTCCAAA ACCTCACGGG CTATAGTCGC 
CACGAGATTG TCGGGCGGAA CTGCCGCTTC TTGCAGGCCC CCGACGGTAA TGTGGAGGCT 
GGCACCAAGC GCGAATTTGT CGAAAACAAC GCCGTATATA CGCTCAAGAA GACCATCGCG 
GAGGGCCAGG AGATTCAGCA GAGTCTGATC AATTATCGGA AGGGCGGCAA GCCTTTCCTG 
AATTTGTTGA CCATGATTCC CATTCCCTGG GATACCGAAG AAATCCGTTA TTTCATTGGA 
TTTCAGATCG ATCTCGTGGA ATGCCCTGAT GCCATAATCG GCCAGGAAGG AAATGGGCCC 
ATGCAGGTCA ACTATACCCA CAGCGATATC GGGCAGTACA TCTGGACGCC ACCTACCCAG 
AAGCAACTGG AGCCTGCCGA CGGCCAGACT TTGGGCGTTG ATGATGTTTC GACACTTTTG 
CAGCAATGTA ATTCGAAGGG TGTTGCATCC GACTGGCACA AACAATCCTG GGATAAGATG 
CTTTTGGAGA ACGCAGACGA TGTTGTCCAT GTGTTGTCGC TCAAGGGTCT CTTCTTATAC 
CTATCTCCGG CCTGTAAGAA AGTGTTGGAG TACGATGCCA GCGACCTCGT TGGGACCTCG 
CTGTCCTCGA TTTGCCACCC GTCCGACATC GTGCCGGTGA CGAGGGAGTT GAAAGAGGCC 
CAGCAACACA CTCCAGTCAA TATAGTCTTC AGAATTCGGA GAAAGAACAG TGGTTATACC 
TGGTTTGAAA GCCACGGAAC ATTGTTCAAC GAACAGGGTA AGGGTCGCAA GTGTATCATT 
CTTGTGGGGA GAAAACGCCC CGTTTTTGCT CTACACAGAA AGGACCTCGA ACTCAACGGC 
GGTATTGGTG ACAGCGAAAT TTGGACCAAG GTCTCGACAT CAGGCATGTT CTTGTTCGTT 
TCGTCGAATG TCCGGTCGCT CCTGGATTTG CTCCCGGAGA ACCTCCAAGG TACCAGCATG 
CAAGATCTCA TGCGCAAGGA ATCCAGAGCC GAGTTTGGGA GGACGATAGA GAAAGCTAGA 
AAGGGCAAGA TCGCGAGCTG CAAGCATGAG GTGCAAAACA AGCGTGGCCA GGTGCTCCAG 
GCCTATACAA CATTCTACCC CGGAGATGGT GGTGAGGGTC AAAGGCCAAC GTTTTTGTTG 
GCGCAAACAA AGCTGTTGAA GGCGTCATCA AGGACACTAG CTCCCGCGAC CGTAACGGTC 
AAGAATATGT CTCCGGGCGG TGTTCCTTTG TCGCCAATGA AAGGGATCCA GACCGACAGT 
GATAGCAACA CTCTCATGGG CGGGATGTCG AAGTCGGGAA GCAGCGATAG TACGGGGGCC 
ATGGTCTCAG CTCGTAGCAG TGCAGGACCC GGCCAGGATG CGGCTTTGGA CGCCGATAAC 
ATATTTGACG AACTCAAGAC AACGCGTTGC ACCAGCTGGC AGTACGAGCT GAGGCAAATG 
GAGAAGGTGA ATCGCATGTT GGCCGAAGAG CTGGCGCAGC TTTTGTCTAA CAAGAAAAAA 
CGGAAACGAA GAAAGGGAGG AGGTAACATG GTGAGGGATT GTGCAAATTG CCATACGAGG 
AACACGCCCG AATGGCGGCG TGGGCCAAGC GGGAATCGAG ATCTATGCAA CAGTTGTGGG 
TTGAGATGGG CAAAGCAGAC TGGGCGCGTA TCGCCGCGCA CCTCATCCCG AGGTGGGAAT 
GGTGATTCGA TGAGCAAGAA GAGCAATTCA CCTAGTCACT CGTCGCCCCT TCATCGAGAA 
GTCGGCAACG ATTCACCGTC TACTACCACC GCTACCAAGA ACTCACCCTC TCTTCGAGGA 
AGTAGCACCA CTGCACCCGG TACGATCACA ACGGACAGCG GACCAGCAGT CGCATCGAGC 
GCTAGTGGAA CCGGTAGTAC GACTATAGCT ACTTCAGCCA ATTCTGCTGC ATCAACCGTC 
AACGCCCTGG GCCCTCCTGC GACAGGTCCC AGTGGCGGCT CACCGGCTCA ACATCTTCCG 
CCTCATCTCC AGGGAACACA CCTTAACGCC CAGGCCATGC AGCGTGTACA CCAGCATAAG 
CAGCATCAGC AGCATCAACA GCAGCATCAA CAGCAGCATC AACAGCAGCA TCAACAGCAG 
CACCAACAGC TTCAGCAGCA TCAGTTCAAC CCTCCACAAA GCCAGCCACT CCTGGAAGGC 
GGAAGCGGTT TCCGTGGAAG CGGAATGGAG ATGACCTCAA TTAGGGAAGA GATGGGCGAA 
CATCAACAGG GCTTAAGTGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011395151.1
CDS	1758..5261
Translation	MNNNYYGSPLSPEELQHQMHQHQQQQQQQQQQQQQQQQQQQQQQQQQQQQHQHQQQQKTNQHRNAGMMNTPPTTNQGNSTIHASDVTMSGGSDSLDEIIQQNLDEMHRRRSVPQPYGGQTRRLSMFDYANPNDGFSDYQLDNMSGNYGDMTGGMGMSGHSSPYAGQNIMAMSDHSGGYSHMSPNVMGNMMTYPNLNMYHSPPIENPYSSAGLDTIRTDFSMDMNMDSGSVSAASVHPTPGLNKQDDEMMTMEQGFGGGDDANASHQAQQNMGGLTPAMTPAMTPAMTPGVSNFAQGMATPVSQDAASTPATTFQSPSLSATTQTIRIGPPPPPSVTNAPTPAPFTSTPSGGGASQTKSIYSKSGFDMLRALWYVASRKDPKLKLGAVDMSCAFVVCDVTLNDCPIIYVSDNFQNLTGYSRHEIVGRNCRFLQAPDGNVEAGTKREFVENNAVYTLKKTIAEGQEIQQSLINYRKGGKPFLNLLTMIPIPWDTEEIRYFIGFQIDLVECPDAIIGQEGNGPMQVNYTHSDIGQYIWTPPTQKQLEPADGQTLGVDDVSTLLQQCNSKGVASDWHKQSWDKMLLENADDVVHVLSLKGLFLYLSPACKKVLEYDASDLVGTSLSSICHPSDIVPVTRELKEAQQHTPVNIVFRIRRKNSGYTWFESHGTLFNEQGKGRKCIILVGRKRPVFALHRKDLELNGGIGDSEIWTKVSTSGMFLFVSSNVRSLLDLLPENLQGTSMQDLMRKESRAEFGRTIEKARKGKIASCKHEVQNKRGQVLQAYTTFYPGDGGEGQRPTFLLAQTKLLKASSRTLAPATVTVKNMSPGGVPLSPMKGIQTDSDSNTLMGGMSKSGSSDSTGAMVSARSSAGPGQDAALDADNIFDELKTTRCTSWQYELRQMEKVNRMLAEELAQLLSNKKKRKRRKGGGNMVRDCANCHTRNTPEWRRGPSGNRDLCNSCGLRWAKQTGRVSPRTSSRGGNGDSMSKKSNSPSHSSPLHREVGNDSPSTTTATKNSPSLRGSSTTAPGTITTDSGPAVASSASGTGSTTIATSANSAASTVNALGPPATGPSGGSPAQHLPPHLQGTHLNAQAMQRVHQHKQHQQHQQQHQQQHQQQHQQQHQQLQQHQFNPPQSQPLLEGGSGFRGSGMEMTSIREEMGEHQQGLSV

Target ORF information:

RefSeq Version	XM_011396849.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A white collar 1 protein (wc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396849.1

ORF Insert Sequence:

ATGAACAACA ACTACTACGG TTCCCCGCTG TCTCCTGAGG AGCTTCAGCA TCAAATGCAC 
CAGCACCAGC AGCAGCAGCA GCAACAACAA CAACAACAAC AGCAGCAGCA GCAGCAGCAA 
CAACAACAAC AGCAACAACA GCAACAACAG CATCAGCATC AGCAGCAACA AAAAACCAAT 
CAGCATCGCA ATGCCGGCAT GATGAATACG CCTCCAACTA CAAATCAAGG AAACAGCACG 
ATTCACGCTT CAGATGTAAC CATGTCAGGA GGAAGCGACT CTCTTGATGA AATCATCCAG 
CAGAACCTCG ACGAAATGCA TCGAAGGCGG AGTGTCCCCC AGCCCTATGG GGGCCAGACT 
AGGAGGTTGT CCATGTTTGA TTACGCGAAC CCCAACGATG GTTTCTCGGA CTATCAACTC 
GATAATATGT CAGGAAATTA CGGGGACATG ACTGGCGGCA TGGGCATGTC AGGGCACTCG 
AGCCCGTATG CAGGGCAGAA CATCATGGCC ATGTCGGACC ACAGCGGTGG ATACTCGCAC 
ATGTCGCCCA ATGTTATGGG CAACATGATG ACTTACCCCA ACCTCAACAT GTACCACTCT 
CCCCCGATCG AGAACCCCTA TTCTTCGGCC GGACTCGACA CTATTCGCAC CGATTTTTCC 
ATGGACATGA ACATGGATAG TGGCTCCGTC AGCGCAGCCA GCGTACATCC CACCCCTGGG 
CTTAATAAGC AGGACGACGA GATGATGACC ATGGAGCAGG GCTTTGGTGG AGGTGACGAT 
GCCAACGCCT CTCACCAGGC CCAACAAAAC ATGGGCGGTT TGACTCCGGC CATGACTCCG 
GCCATGACCC CTGCAATGAC CCCGGGTGTC AGCAACTTTG CACAAGGCAT GGCTACACCA 
GTCTCACAGG ATGCGGCAAG CACACCCGCG ACAACCTTCC AGTCTCCGTC CTTATCCGCA 
ACAACACAGA CAATACGAAT TGGGCCGCCG CCACCGCCGA GCGTCACCAA TGCCCCCACC 
CCAGCGCCAT TTACCTCGAC GCCGTCAGGT GGAGGAGCGT CCCAGACAAA GAGCATTTAC 
TCCAAAAGCG GTTTCGACAT GCTGAGGGCG TTGTGGTATG TGGCATCGAG GAAGGACCCA 
AAGCTTAAGC TCGGTGCCGT GGACATGTCT TGTGCATTTG TCGTCTGTGA TGTAACACTC 
AACGATTGCC CCATCATTTA TGTGTCCGAT AACTTCCAAA ACCTCACGGG CTATAGTCGC 
CACGAGATTG TCGGGCGGAA CTGCCGCTTC TTGCAGGCCC CCGACGGTAA TGTGGAGGCT 
GGCACCAAGC GCGAATTTGT CGAAAACAAC GCCGTATATA CGCTCAAGAA GACCATCGCG 
GAGGGCCAGG AGATTCAGCA GAGTCTGATC AATTATCGGA AGGGCGGCAA GCCTTTCCTG 
AATTTGTTGA CCATGATTCC CATTCCCTGG GATACCGAAG AAATCCGTTA TTTCATTGGA 
TTTCAGATCG ATCTCGTGGA ATGCCCTGAT GCCATAATCG GCCAGGAAGG AAATGGGCCC 
ATGCAGGTCA ACTATACCCA CAGCGATATC GGGCAGTACA TCTGGACGCC ACCTACCCAG 
AAGCAACTGG AGCCTGCCGA CGGCCAGACT TTGGGCGTTG ATGATGTTTC GACACTTTTG 
CAGCAATGTA ATTCGAAGGG TGTTGCATCC GACTGGCACA AACAATCCTG GGATAAGATG 
CTTTTGGAGA ACGCAGACGA TGTTGTCCAT GTGTTGTCGC TCAAGGGTCT CTTCTTATAC 
CTATCTCCGG CCTGTAAGAA AGTGTTGGAG TACGATGCCA GCGACCTCGT TGGGACCTCG 
CTGTCCTCGA TTTGCCACCC GTCCGACATC GTGCCGGTGA CGAGGGAGTT GAAAGAGGCC 
CAGCAACACA CTCCAGTCAA TATAGTCTTC AGAATTCGGA GAAAGAACAG TGGTTATACC 
TGGTTTGAAA GCCACGGAAC ATTGTTCAAC GAACAGGGTA AGGGTCGCAA GTGTATCATT 
CTTGTGGGGA GAAAACGCCC CGTTTTTGCT CTACACAGAA AGGACCTCGA ACTCAACGGC 
GGTATTGGTG ACAGCGAAAT TTGGACCAAG GTCTCGACAT CAGGCATGTT CTTGTTCGTT 
TCGTCGAATG TCCGGTCGCT CCTGGATTTG CTCCCGGAGA ACCTCCAAGG TACCAGCATG 
CAAGATCTCA TGCGCAAGGA ATCCAGAGCC GAGTTTGGGA GGACGATAGA GAAAGCTAGA 
AAGGGCAAGA TCGCGAGCTG CAAGCATGAG GTGCAAAACA AGCGTGGCCA GGTGCTCCAG 
GCCTATACAA CATTCTACCC CGGAGATGGT GGTGAGGGTC AAAGGCCAAC GTTTTTGTTG 
GCGCAAACAA AGCTGTTGAA GGCGTCATCA AGGACACTAG CTCCCGCGAC CGTAACGGTC 
AAGAATATGT CTCCGGGCGG TGTTCCTTTG TCGCCAATGA AAGGGATCCA GACCGACAGT 
GATAGCAACA CTCTCATGGG CGGGATGTCG AAGTCGGGAA GCAGCGATAG TACGGGGGCC 
ATGGTCTCAG CTCGTAGCAG TGCAGGACCC GGCCAGGATG CGGCTTTGGA CGCCGATAAC 
ATATTTGACG AACTCAAGAC AACGCGTTGC ACCAGCTGGC AGTACGAGCT GAGGCAAATG 
GAGAAGGTGA ATCGCATGTT GGCCGAAGAG CTGGCGCAGC TTTTGTCTAA CAAGAAAAAA 
CGGAAACGAA GAAAGGGAGG AGGTAACATG GTGAGGGATT GTGCAAATTG CCATACGAGG 
AACACGCCCG AATGGCGGCG TGGGCCAAGC GGGAATCGAG ATCTATGCAA CAGTTGTGGG 
TTGAGATGGG CAAAGCAGAC TGGGCGCGTA TCGCCGCGCA CCTCATCCCG AGGTGGGAAT 
GGTGATTCGA TGAGCAAGAA GAGCAATTCA CCTAGTCACT CGTCGCCCCT TCATCGAGAA 
GTCGGCAACG ATTCACCGTC TACTACCACC GCTACCAAGA ACTCACCCTC TCTTCGAGGA 
AGTAGCACCA CTGCACCCGG TACGATCACA ACGGACAGCG GACCAGCAGT CGCATCGAGC 
GCTAGTGGAA CCGGTAGTAC GACTATAGCT ACTTCAGCCA ATTCTGCTGC ATCAACCGTC 
AACGCCCTGG GCCCTCCTGC GACAGGTCCC AGTGGCGGCT CACCGGCTCA ACATCTTCCG 
CCTCATCTCC AGGGAACACA CCTTAACGCC CAGGCCATGC AGCGTGTACA CCAGCATAAG 
CAGCATCAGC AGCATCAACA GCAGCATCAA CAGCAGCATC AACAGCAGCA TCAACAGCAG 
CACCAACAGC TTCAGCAGCA TCAGTTCAAC CCTCCACAAA GCCAGCCACT CCTGGAAGGC 
GGAAGCGGTT TCCGTGGAAG CGGAATGGAG ATGACCTCAA TTAGGGAAGA GATGGGCGAA 
CATCAACAGG GCTTAAGTGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wc-1 ( XM_011396851.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011395153.1 Neurospora crassa OR74A white collar 1 protein, variant 3 (wc-1), mRNA.

Price & Availability↑

CloneID	ONf11183
Clone ID Related Accession (Same CDS sequence)	XM_011396849.1 , XM_011396851.1 , XM_011396850.1 , XM_011396848.1
Accession Version	XM_011396851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	white collar 1 protein, variant 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507).

ATGAACAACA ACTACTACGG TTCCCCGCTG TCTCCTGAGG AGCTTCAGCA TCAAATGCAC 
CAGCACCAGC AGCAGCAGCA GCAACAACAA CAACAACAAC AGCAGCAGCA GCAGCAGCAA 
CAACAACAAC AGCAACAACA GCAACAACAG CATCAGCATC AGCAGCAACA AAAAACCAAT 
CAGCATCGCA ATGCCGGCAT GATGAATACG CCTCCAACTA CAAATCAAGG AAACAGCACG 
ATTCACGCTT CAGATGTAAC CATGTCAGGA GGAAGCGACT CTCTTGATGA AATCATCCAG 
CAGAACCTCG ACGAAATGCA TCGAAGGCGG AGTGTCCCCC AGCCCTATGG GGGCCAGACT 
AGGAGGTTGT CCATGTTTGA TTACGCGAAC CCCAACGATG GTTTCTCGGA CTATCAACTC 
GATAATATGT CAGGAAATTA CGGGGACATG ACTGGCGGCA TGGGCATGTC AGGGCACTCG 
AGCCCGTATG CAGGGCAGAA CATCATGGCC ATGTCGGACC ACAGCGGTGG ATACTCGCAC 
ATGTCGCCCA ATGTTATGGG CAACATGATG ACTTACCCCA ACCTCAACAT GTACCACTCT 
CCCCCGATCG AGAACCCCTA TTCTTCGGCC GGACTCGACA CTATTCGCAC CGATTTTTCC 
ATGGACATGA ACATGGATAG TGGCTCCGTC AGCGCAGCCA GCGTACATCC CACCCCTGGG 
CTTAATAAGC AGGACGACGA GATGATGACC ATGGAGCAGG GCTTTGGTGG AGGTGACGAT 
GCCAACGCCT CTCACCAGGC CCAACAAAAC ATGGGCGGTT TGACTCCGGC CATGACTCCG 
GCCATGACCC CTGCAATGAC CCCGGGTGTC AGCAACTTTG CACAAGGCAT GGCTACACCA 
GTCTCACAGG ATGCGGCAAG CACACCCGCG ACAACCTTCC AGTCTCCGTC CTTATCCGCA 
ACAACACAGA CAATACGAAT TGGGCCGCCG CCACCGCCGA GCGTCACCAA TGCCCCCACC 
CCAGCGCCAT TTACCTCGAC GCCGTCAGGT GGAGGAGCGT CCCAGACAAA GAGCATTTAC 
TCCAAAAGCG GTTTCGACAT GCTGAGGGCG TTGTGGTATG TGGCATCGAG GAAGGACCCA 
AAGCTTAAGC TCGGTGCCGT GGACATGTCT TGTGCATTTG TCGTCTGTGA TGTAACACTC 
AACGATTGCC CCATCATTTA TGTGTCCGAT AACTTCCAAA ACCTCACGGG CTATAGTCGC 
CACGAGATTG TCGGGCGGAA CTGCCGCTTC TTGCAGGCCC CCGACGGTAA TGTGGAGGCT 
GGCACCAAGC GCGAATTTGT CGAAAACAAC GCCGTATATA CGCTCAAGAA GACCATCGCG 
GAGGGCCAGG AGATTCAGCA GAGTCTGATC AATTATCGGA AGGGCGGCAA GCCTTTCCTG 
AATTTGTTGA CCATGATTCC CATTCCCTGG GATACCGAAG AAATCCGTTA TTTCATTGGA 
TTTCAGATCG ATCTCGTGGA ATGCCCTGAT GCCATAATCG GCCAGGAAGG AAATGGGCCC 
ATGCAGGTCA ACTATACCCA CAGCGATATC GGGCAGTACA TCTGGACGCC ACCTACCCAG 
AAGCAACTGG AGCCTGCCGA CGGCCAGACT TTGGGCGTTG ATGATGTTTC GACACTTTTG 
CAGCAATGTA ATTCGAAGGG TGTTGCATCC GACTGGCACA AACAATCCTG GGATAAGATG 
CTTTTGGAGA ACGCAGACGA TGTTGTCCAT GTGTTGTCGC TCAAGGGTCT CTTCTTATAC 
CTATCTCCGG CCTGTAAGAA AGTGTTGGAG TACGATGCCA GCGACCTCGT TGGGACCTCG 
CTGTCCTCGA TTTGCCACCC GTCCGACATC GTGCCGGTGA CGAGGGAGTT GAAAGAGGCC 
CAGCAACACA CTCCAGTCAA TATAGTCTTC AGAATTCGGA GAAAGAACAG TGGTTATACC 
TGGTTTGAAA GCCACGGAAC ATTGTTCAAC GAACAGGGTA AGGGTCGCAA GTGTATCATT 
CTTGTGGGGA GAAAACGCCC CGTTTTTGCT CTACACAGAA AGGACCTCGA ACTCAACGGC 
GGTATTGGTG ACAGCGAAAT TTGGACCAAG GTCTCGACAT CAGGCATGTT CTTGTTCGTT 
TCGTCGAATG TCCGGTCGCT CCTGGATTTG CTCCCGGAGA ACCTCCAAGG TACCAGCATG 
CAAGATCTCA TGCGCAAGGA ATCCAGAGCC GAGTTTGGGA GGACGATAGA GAAAGCTAGA 
AAGGGCAAGA TCGCGAGCTG CAAGCATGAG GTGCAAAACA AGCGTGGCCA GGTGCTCCAG 
GCCTATACAA CATTCTACCC CGGAGATGGT GGTGAGGGTC AAAGGCCAAC GTTTTTGTTG 
GCGCAAACAA AGCTGTTGAA GGCGTCATCA AGGACACTAG CTCCCGCGAC CGTAACGGTC 
AAGAATATGT CTCCGGGCGG TGTTCCTTTG TCGCCAATGA AAGGGATCCA GACCGACAGT 
GATAGCAACA CTCTCATGGG CGGGATGTCG AAGTCGGGAA GCAGCGATAG TACGGGGGCC 
ATGGTCTCAG CTCGTAGCAG TGCAGGACCC GGCCAGGATG CGGCTTTGGA CGCCGATAAC 
ATATTTGACG AACTCAAGAC AACGCGTTGC ACCAGCTGGC AGTACGAGCT GAGGCAAATG 
GAGAAGGTGA ATCGCATGTT GGCCGAAGAG CTGGCGCAGC TTTTGTCTAA CAAGAAAAAA 
CGGAAACGAA GAAAGGGAGG AGGTAACATG GTGAGGGATT GTGCAAATTG CCATACGAGG 
AACACGCCCG AATGGCGGCG TGGGCCAAGC GGGAATCGAG ATCTATGCAA CAGTTGTGGG 
TTGAGATGGG CAAAGCAGAC TGGGCGCGTA TCGCCGCGCA CCTCATCCCG AGGTGGGAAT 
GGTGATTCGA TGAGCAAGAA GAGCAATTCA CCTAGTCACT CGTCGCCCCT TCATCGAGAA 
GTCGGCAACG ATTCACCGTC TACTACCACC GCTACCAAGA ACTCACCCTC TCTTCGAGGA 
AGTAGCACCA CTGCACCCGG TACGATCACA ACGGACAGCG GACCAGCAGT CGCATCGAGC 
GCTAGTGGAA CCGGTAGTAC GACTATAGCT ACTTCAGCCA ATTCTGCTGC ATCAACCGTC 
AACGCCCTGG GCCCTCCTGC GACAGGTCCC AGTGGCGGCT CACCGGCTCA ACATCTTCCG 
CCTCATCTCC AGGGAACACA CCTTAACGCC CAGGCCATGC AGCGTGTACA CCAGCATAAG 
CAGCATCAGC AGCATCAACA GCAGCATCAA CAGCAGCATC AACAGCAGCA TCAACAGCAG 
CACCAACAGC TTCAGCAGCA TCAGTTCAAC CCTCCACAAA GCCAGCCACT CCTGGAAGGC 
GGAAGCGGTT TCCGTGGAAG CGGAATGGAG ATGACCTCAA TTAGGGAAGA GATGGGCGAA 
CATCAACAGG GCTTAAGTGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011395153.1
CDS	470..3973
Translation	MNNNYYGSPLSPEELQHQMHQHQQQQQQQQQQQQQQQQQQQQQQQQQQQQHQHQQQQKTNQHRNAGMMNTPPTTNQGNSTIHASDVTMSGGSDSLDEIIQQNLDEMHRRRSVPQPYGGQTRRLSMFDYANPNDGFSDYQLDNMSGNYGDMTGGMGMSGHSSPYAGQNIMAMSDHSGGYSHMSPNVMGNMMTYPNLNMYHSPPIENPYSSAGLDTIRTDFSMDMNMDSGSVSAASVHPTPGLNKQDDEMMTMEQGFGGGDDANASHQAQQNMGGLTPAMTPAMTPAMTPGVSNFAQGMATPVSQDAASTPATTFQSPSLSATTQTIRIGPPPPPSVTNAPTPAPFTSTPSGGGASQTKSIYSKSGFDMLRALWYVASRKDPKLKLGAVDMSCAFVVCDVTLNDCPIIYVSDNFQNLTGYSRHEIVGRNCRFLQAPDGNVEAGTKREFVENNAVYTLKKTIAEGQEIQQSLINYRKGGKPFLNLLTMIPIPWDTEEIRYFIGFQIDLVECPDAIIGQEGNGPMQVNYTHSDIGQYIWTPPTQKQLEPADGQTLGVDDVSTLLQQCNSKGVASDWHKQSWDKMLLENADDVVHVLSLKGLFLYLSPACKKVLEYDASDLVGTSLSSICHPSDIVPVTRELKEAQQHTPVNIVFRIRRKNSGYTWFESHGTLFNEQGKGRKCIILVGRKRPVFALHRKDLELNGGIGDSEIWTKVSTSGMFLFVSSNVRSLLDLLPENLQGTSMQDLMRKESRAEFGRTIEKARKGKIASCKHEVQNKRGQVLQAYTTFYPGDGGEGQRPTFLLAQTKLLKASSRTLAPATVTVKNMSPGGVPLSPMKGIQTDSDSNTLMGGMSKSGSSDSTGAMVSARSSAGPGQDAALDADNIFDELKTTRCTSWQYELRQMEKVNRMLAEELAQLLSNKKKRKRRKGGGNMVRDCANCHTRNTPEWRRGPSGNRDLCNSCGLRWAKQTGRVSPRTSSRGGNGDSMSKKSNSPSHSSPLHREVGNDSPSTTTATKNSPSLRGSSTTAPGTITTDSGPAVASSASGTGSTTIATSANSAASTVNALGPPATGPSGGSPAQHLPPHLQGTHLNAQAMQRVHQHKQHQQHQQQHQQQHQQQHQQQHQQLQQHQFNPPQSQPLLEGGSGFRGSGMEMTSIREEMGEHQQGLSV

Target ORF information:

RefSeq Version	XM_011396851.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A white collar 1 protein, variant 3 (wc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396851.1

ORF Insert Sequence:

ATGAACAACA ACTACTACGG TTCCCCGCTG TCTCCTGAGG AGCTTCAGCA TCAAATGCAC 
CAGCACCAGC AGCAGCAGCA GCAACAACAA CAACAACAAC AGCAGCAGCA GCAGCAGCAA 
CAACAACAAC AGCAACAACA GCAACAACAG CATCAGCATC AGCAGCAACA AAAAACCAAT 
CAGCATCGCA ATGCCGGCAT GATGAATACG CCTCCAACTA CAAATCAAGG AAACAGCACG 
ATTCACGCTT CAGATGTAAC CATGTCAGGA GGAAGCGACT CTCTTGATGA AATCATCCAG 
CAGAACCTCG ACGAAATGCA TCGAAGGCGG AGTGTCCCCC AGCCCTATGG GGGCCAGACT 
AGGAGGTTGT CCATGTTTGA TTACGCGAAC CCCAACGATG GTTTCTCGGA CTATCAACTC 
GATAATATGT CAGGAAATTA CGGGGACATG ACTGGCGGCA TGGGCATGTC AGGGCACTCG 
AGCCCGTATG CAGGGCAGAA CATCATGGCC ATGTCGGACC ACAGCGGTGG ATACTCGCAC 
ATGTCGCCCA ATGTTATGGG CAACATGATG ACTTACCCCA ACCTCAACAT GTACCACTCT 
CCCCCGATCG AGAACCCCTA TTCTTCGGCC GGACTCGACA CTATTCGCAC CGATTTTTCC 
ATGGACATGA ACATGGATAG TGGCTCCGTC AGCGCAGCCA GCGTACATCC CACCCCTGGG 
CTTAATAAGC AGGACGACGA GATGATGACC ATGGAGCAGG GCTTTGGTGG AGGTGACGAT 
GCCAACGCCT CTCACCAGGC CCAACAAAAC ATGGGCGGTT TGACTCCGGC CATGACTCCG 
GCCATGACCC CTGCAATGAC CCCGGGTGTC AGCAACTTTG CACAAGGCAT GGCTACACCA 
GTCTCACAGG ATGCGGCAAG CACACCCGCG ACAACCTTCC AGTCTCCGTC CTTATCCGCA 
ACAACACAGA CAATACGAAT TGGGCCGCCG CCACCGCCGA GCGTCACCAA TGCCCCCACC 
CCAGCGCCAT TTACCTCGAC GCCGTCAGGT GGAGGAGCGT CCCAGACAAA GAGCATTTAC 
TCCAAAAGCG GTTTCGACAT GCTGAGGGCG TTGTGGTATG TGGCATCGAG GAAGGACCCA 
AAGCTTAAGC TCGGTGCCGT GGACATGTCT TGTGCATTTG TCGTCTGTGA TGTAACACTC 
AACGATTGCC CCATCATTTA TGTGTCCGAT AACTTCCAAA ACCTCACGGG CTATAGTCGC 
CACGAGATTG TCGGGCGGAA CTGCCGCTTC TTGCAGGCCC CCGACGGTAA TGTGGAGGCT 
GGCACCAAGC GCGAATTTGT CGAAAACAAC GCCGTATATA CGCTCAAGAA GACCATCGCG 
GAGGGCCAGG AGATTCAGCA GAGTCTGATC AATTATCGGA AGGGCGGCAA GCCTTTCCTG 
AATTTGTTGA CCATGATTCC CATTCCCTGG GATACCGAAG AAATCCGTTA TTTCATTGGA 
TTTCAGATCG ATCTCGTGGA ATGCCCTGAT GCCATAATCG GCCAGGAAGG AAATGGGCCC 
ATGCAGGTCA ACTATACCCA CAGCGATATC GGGCAGTACA TCTGGACGCC ACCTACCCAG 
AAGCAACTGG AGCCTGCCGA CGGCCAGACT TTGGGCGTTG ATGATGTTTC GACACTTTTG 
CAGCAATGTA ATTCGAAGGG TGTTGCATCC GACTGGCACA AACAATCCTG GGATAAGATG 
CTTTTGGAGA ACGCAGACGA TGTTGTCCAT GTGTTGTCGC TCAAGGGTCT CTTCTTATAC 
CTATCTCCGG CCTGTAAGAA AGTGTTGGAG TACGATGCCA GCGACCTCGT TGGGACCTCG 
CTGTCCTCGA TTTGCCACCC GTCCGACATC GTGCCGGTGA CGAGGGAGTT GAAAGAGGCC 
CAGCAACACA CTCCAGTCAA TATAGTCTTC AGAATTCGGA GAAAGAACAG TGGTTATACC 
TGGTTTGAAA GCCACGGAAC ATTGTTCAAC GAACAGGGTA AGGGTCGCAA GTGTATCATT 
CTTGTGGGGA GAAAACGCCC CGTTTTTGCT CTACACAGAA AGGACCTCGA ACTCAACGGC 
GGTATTGGTG ACAGCGAAAT TTGGACCAAG GTCTCGACAT CAGGCATGTT CTTGTTCGTT 
TCGTCGAATG TCCGGTCGCT CCTGGATTTG CTCCCGGAGA ACCTCCAAGG TACCAGCATG 
CAAGATCTCA TGCGCAAGGA ATCCAGAGCC GAGTTTGGGA GGACGATAGA GAAAGCTAGA 
AAGGGCAAGA TCGCGAGCTG CAAGCATGAG GTGCAAAACA AGCGTGGCCA GGTGCTCCAG 
GCCTATACAA CATTCTACCC CGGAGATGGT GGTGAGGGTC AAAGGCCAAC GTTTTTGTTG 
GCGCAAACAA AGCTGTTGAA GGCGTCATCA AGGACACTAG CTCCCGCGAC CGTAACGGTC 
AAGAATATGT CTCCGGGCGG TGTTCCTTTG TCGCCAATGA AAGGGATCCA GACCGACAGT 
GATAGCAACA CTCTCATGGG CGGGATGTCG AAGTCGGGAA GCAGCGATAG TACGGGGGCC 
ATGGTCTCAG CTCGTAGCAG TGCAGGACCC GGCCAGGATG CGGCTTTGGA CGCCGATAAC 
ATATTTGACG AACTCAAGAC AACGCGTTGC ACCAGCTGGC AGTACGAGCT GAGGCAAATG 
GAGAAGGTGA ATCGCATGTT GGCCGAAGAG CTGGCGCAGC TTTTGTCTAA CAAGAAAAAA 
CGGAAACGAA GAAAGGGAGG AGGTAACATG GTGAGGGATT GTGCAAATTG CCATACGAGG 
AACACGCCCG AATGGCGGCG TGGGCCAAGC GGGAATCGAG ATCTATGCAA CAGTTGTGGG 
TTGAGATGGG CAAAGCAGAC TGGGCGCGTA TCGCCGCGCA CCTCATCCCG AGGTGGGAAT 
GGTGATTCGA TGAGCAAGAA GAGCAATTCA CCTAGTCACT CGTCGCCCCT TCATCGAGAA 
GTCGGCAACG ATTCACCGTC TACTACCACC GCTACCAAGA ACTCACCCTC TCTTCGAGGA 
AGTAGCACCA CTGCACCCGG TACGATCACA ACGGACAGCG GACCAGCAGT CGCATCGAGC 
GCTAGTGGAA CCGGTAGTAC GACTATAGCT ACTTCAGCCA ATTCTGCTGC ATCAACCGTC 
AACGCCCTGG GCCCTCCTGC GACAGGTCCC AGTGGCGGCT CACCGGCTCA ACATCTTCCG 
CCTCATCTCC AGGGAACACA CCTTAACGCC CAGGCCATGC AGCGTGTACA CCAGCATAAG 
CAGCATCAGC AGCATCAACA GCAGCATCAA CAGCAGCATC AACAGCAGCA TCAACAGCAG 
CACCAACAGC TTCAGCAGCA TCAGTTCAAC CCTCCACAAA GCCAGCCACT CCTGGAAGGC 
GGAAGCGGTT TCCGTGGAAG CGGAATGGAG ATGACCTCAA TTAGGGAAGA GATGGGCGAA 
CATCAACAGG GCTTAAGTGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wc-1 ( XM_011396850.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011395152.1 Neurospora crassa OR74A white collar 1 protein, variant 1 (wc-1), mRNA.

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CloneID	ONf11183
Clone ID Related Accession (Same CDS sequence)	XM_011396849.1 , XM_011396851.1 , XM_011396850.1 , XM_011396848.1
Accession Version	XM_011396850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	white collar 1 protein, variant 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507).

ATGAACAACA ACTACTACGG TTCCCCGCTG TCTCCTGAGG AGCTTCAGCA TCAAATGCAC 
CAGCACCAGC AGCAGCAGCA GCAACAACAA CAACAACAAC AGCAGCAGCA GCAGCAGCAA 
CAACAACAAC AGCAACAACA GCAACAACAG CATCAGCATC AGCAGCAACA AAAAACCAAT 
CAGCATCGCA ATGCCGGCAT GATGAATACG CCTCCAACTA CAAATCAAGG AAACAGCACG 
ATTCACGCTT CAGATGTAAC CATGTCAGGA GGAAGCGACT CTCTTGATGA AATCATCCAG 
CAGAACCTCG ACGAAATGCA TCGAAGGCGG AGTGTCCCCC AGCCCTATGG GGGCCAGACT 
AGGAGGTTGT CCATGTTTGA TTACGCGAAC CCCAACGATG GTTTCTCGGA CTATCAACTC 
GATAATATGT CAGGAAATTA CGGGGACATG ACTGGCGGCA TGGGCATGTC AGGGCACTCG 
AGCCCGTATG CAGGGCAGAA CATCATGGCC ATGTCGGACC ACAGCGGTGG ATACTCGCAC 
ATGTCGCCCA ATGTTATGGG CAACATGATG ACTTACCCCA ACCTCAACAT GTACCACTCT 
CCCCCGATCG AGAACCCCTA TTCTTCGGCC GGACTCGACA CTATTCGCAC CGATTTTTCC 
ATGGACATGA ACATGGATAG TGGCTCCGTC AGCGCAGCCA GCGTACATCC CACCCCTGGG 
CTTAATAAGC AGGACGACGA GATGATGACC ATGGAGCAGG GCTTTGGTGG AGGTGACGAT 
GCCAACGCCT CTCACCAGGC CCAACAAAAC ATGGGCGGTT TGACTCCGGC CATGACTCCG 
GCCATGACCC CTGCAATGAC CCCGGGTGTC AGCAACTTTG CACAAGGCAT GGCTACACCA 
GTCTCACAGG ATGCGGCAAG CACACCCGCG ACAACCTTCC AGTCTCCGTC CTTATCCGCA 
ACAACACAGA CAATACGAAT TGGGCCGCCG CCACCGCCGA GCGTCACCAA TGCCCCCACC 
CCAGCGCCAT TTACCTCGAC GCCGTCAGGT GGAGGAGCGT CCCAGACAAA GAGCATTTAC 
TCCAAAAGCG GTTTCGACAT GCTGAGGGCG TTGTGGTATG TGGCATCGAG GAAGGACCCA 
AAGCTTAAGC TCGGTGCCGT GGACATGTCT TGTGCATTTG TCGTCTGTGA TGTAACACTC 
AACGATTGCC CCATCATTTA TGTGTCCGAT AACTTCCAAA ACCTCACGGG CTATAGTCGC 
CACGAGATTG TCGGGCGGAA CTGCCGCTTC TTGCAGGCCC CCGACGGTAA TGTGGAGGCT 
GGCACCAAGC GCGAATTTGT CGAAAACAAC GCCGTATATA CGCTCAAGAA GACCATCGCG 
GAGGGCCAGG AGATTCAGCA GAGTCTGATC AATTATCGGA AGGGCGGCAA GCCTTTCCTG 
AATTTGTTGA CCATGATTCC CATTCCCTGG GATACCGAAG AAATCCGTTA TTTCATTGGA 
TTTCAGATCG ATCTCGTGGA ATGCCCTGAT GCCATAATCG GCCAGGAAGG AAATGGGCCC 
ATGCAGGTCA ACTATACCCA CAGCGATATC GGGCAGTACA TCTGGACGCC ACCTACCCAG 
AAGCAACTGG AGCCTGCCGA CGGCCAGACT TTGGGCGTTG ATGATGTTTC GACACTTTTG 
CAGCAATGTA ATTCGAAGGG TGTTGCATCC GACTGGCACA AACAATCCTG GGATAAGATG 
CTTTTGGAGA ACGCAGACGA TGTTGTCCAT GTGTTGTCGC TCAAGGGTCT CTTCTTATAC 
CTATCTCCGG CCTGTAAGAA AGTGTTGGAG TACGATGCCA GCGACCTCGT TGGGACCTCG 
CTGTCCTCGA TTTGCCACCC GTCCGACATC GTGCCGGTGA CGAGGGAGTT GAAAGAGGCC 
CAGCAACACA CTCCAGTCAA TATAGTCTTC AGAATTCGGA GAAAGAACAG TGGTTATACC 
TGGTTTGAAA GCCACGGAAC ATTGTTCAAC GAACAGGGTA AGGGTCGCAA GTGTATCATT 
CTTGTGGGGA GAAAACGCCC CGTTTTTGCT CTACACAGAA AGGACCTCGA ACTCAACGGC 
GGTATTGGTG ACAGCGAAAT TTGGACCAAG GTCTCGACAT CAGGCATGTT CTTGTTCGTT 
TCGTCGAATG TCCGGTCGCT CCTGGATTTG CTCCCGGAGA ACCTCCAAGG TACCAGCATG 
CAAGATCTCA TGCGCAAGGA ATCCAGAGCC GAGTTTGGGA GGACGATAGA GAAAGCTAGA 
AAGGGCAAGA TCGCGAGCTG CAAGCATGAG GTGCAAAACA AGCGTGGCCA GGTGCTCCAG 
GCCTATACAA CATTCTACCC CGGAGATGGT GGTGAGGGTC AAAGGCCAAC GTTTTTGTTG 
GCGCAAACAA AGCTGTTGAA GGCGTCATCA AGGACACTAG CTCCCGCGAC CGTAACGGTC 
AAGAATATGT CTCCGGGCGG TGTTCCTTTG TCGCCAATGA AAGGGATCCA GACCGACAGT 
GATAGCAACA CTCTCATGGG CGGGATGTCG AAGTCGGGAA GCAGCGATAG TACGGGGGCC 
ATGGTCTCAG CTCGTAGCAG TGCAGGACCC GGCCAGGATG CGGCTTTGGA CGCCGATAAC 
ATATTTGACG AACTCAAGAC AACGCGTTGC ACCAGCTGGC AGTACGAGCT GAGGCAAATG 
GAGAAGGTGA ATCGCATGTT GGCCGAAGAG CTGGCGCAGC TTTTGTCTAA CAAGAAAAAA 
CGGAAACGAA GAAAGGGAGG AGGTAACATG GTGAGGGATT GTGCAAATTG CCATACGAGG 
AACACGCCCG AATGGCGGCG TGGGCCAAGC GGGAATCGAG ATCTATGCAA CAGTTGTGGG 
TTGAGATGGG CAAAGCAGAC TGGGCGCGTA TCGCCGCGCA CCTCATCCCG AGGTGGGAAT 
GGTGATTCGA TGAGCAAGAA GAGCAATTCA CCTAGTCACT CGTCGCCCCT TCATCGAGAA 
GTCGGCAACG ATTCACCGTC TACTACCACC GCTACCAAGA ACTCACCCTC TCTTCGAGGA 
AGTAGCACCA CTGCACCCGG TACGATCACA ACGGACAGCG GACCAGCAGT CGCATCGAGC 
GCTAGTGGAA CCGGTAGTAC GACTATAGCT ACTTCAGCCA ATTCTGCTGC ATCAACCGTC 
AACGCCCTGG GCCCTCCTGC GACAGGTCCC AGTGGCGGCT CACCGGCTCA ACATCTTCCG 
CCTCATCTCC AGGGAACACA CCTTAACGCC CAGGCCATGC AGCGTGTACA CCAGCATAAG 
CAGCATCAGC AGCATCAACA GCAGCATCAA CAGCAGCATC AACAGCAGCA TCAACAGCAG 
CACCAACAGC TTCAGCAGCA TCAGTTCAAC CCTCCACAAA GCCAGCCACT CCTGGAAGGC 
GGAAGCGGTT TCCGTGGAAG CGGAATGGAG ATGACCTCAA TTAGGGAAGA GATGGGCGAA 
CATCAACAGG GCTTAAGTGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011395152.1
CDS	773..4276
Translation	MNNNYYGSPLSPEELQHQMHQHQQQQQQQQQQQQQQQQQQQQQQQQQQQQHQHQQQQKTNQHRNAGMMNTPPTTNQGNSTIHASDVTMSGGSDSLDEIIQQNLDEMHRRRSVPQPYGGQTRRLSMFDYANPNDGFSDYQLDNMSGNYGDMTGGMGMSGHSSPYAGQNIMAMSDHSGGYSHMSPNVMGNMMTYPNLNMYHSPPIENPYSSAGLDTIRTDFSMDMNMDSGSVSAASVHPTPGLNKQDDEMMTMEQGFGGGDDANASHQAQQNMGGLTPAMTPAMTPAMTPGVSNFAQGMATPVSQDAASTPATTFQSPSLSATTQTIRIGPPPPPSVTNAPTPAPFTSTPSGGGASQTKSIYSKSGFDMLRALWYVASRKDPKLKLGAVDMSCAFVVCDVTLNDCPIIYVSDNFQNLTGYSRHEIVGRNCRFLQAPDGNVEAGTKREFVENNAVYTLKKTIAEGQEIQQSLINYRKGGKPFLNLLTMIPIPWDTEEIRYFIGFQIDLVECPDAIIGQEGNGPMQVNYTHSDIGQYIWTPPTQKQLEPADGQTLGVDDVSTLLQQCNSKGVASDWHKQSWDKMLLENADDVVHVLSLKGLFLYLSPACKKVLEYDASDLVGTSLSSICHPSDIVPVTRELKEAQQHTPVNIVFRIRRKNSGYTWFESHGTLFNEQGKGRKCIILVGRKRPVFALHRKDLELNGGIGDSEIWTKVSTSGMFLFVSSNVRSLLDLLPENLQGTSMQDLMRKESRAEFGRTIEKARKGKIASCKHEVQNKRGQVLQAYTTFYPGDGGEGQRPTFLLAQTKLLKASSRTLAPATVTVKNMSPGGVPLSPMKGIQTDSDSNTLMGGMSKSGSSDSTGAMVSARSSAGPGQDAALDADNIFDELKTTRCTSWQYELRQMEKVNRMLAEELAQLLSNKKKRKRRKGGGNMVRDCANCHTRNTPEWRRGPSGNRDLCNSCGLRWAKQTGRVSPRTSSRGGNGDSMSKKSNSPSHSSPLHREVGNDSPSTTTATKNSPSLRGSSTTAPGTITTDSGPAVASSASGTGSTTIATSANSAASTVNALGPPATGPSGGSPAQHLPPHLQGTHLNAQAMQRVHQHKQHQQHQQQHQQQHQQQHQQQHQQLQQHQFNPPQSQPLLEGGSGFRGSGMEMTSIREEMGEHQQGLSV

Target ORF information:

RefSeq Version	XM_011396850.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A white collar 1 protein, variant 1 (wc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396850.1

ORF Insert Sequence:

ATGAACAACA ACTACTACGG TTCCCCGCTG TCTCCTGAGG AGCTTCAGCA TCAAATGCAC 
CAGCACCAGC AGCAGCAGCA GCAACAACAA CAACAACAAC AGCAGCAGCA GCAGCAGCAA 
CAACAACAAC AGCAACAACA GCAACAACAG CATCAGCATC AGCAGCAACA AAAAACCAAT 
CAGCATCGCA ATGCCGGCAT GATGAATACG CCTCCAACTA CAAATCAAGG AAACAGCACG 
ATTCACGCTT CAGATGTAAC CATGTCAGGA GGAAGCGACT CTCTTGATGA AATCATCCAG 
CAGAACCTCG ACGAAATGCA TCGAAGGCGG AGTGTCCCCC AGCCCTATGG GGGCCAGACT 
AGGAGGTTGT CCATGTTTGA TTACGCGAAC CCCAACGATG GTTTCTCGGA CTATCAACTC 
GATAATATGT CAGGAAATTA CGGGGACATG ACTGGCGGCA TGGGCATGTC AGGGCACTCG 
AGCCCGTATG CAGGGCAGAA CATCATGGCC ATGTCGGACC ACAGCGGTGG ATACTCGCAC 
ATGTCGCCCA ATGTTATGGG CAACATGATG ACTTACCCCA ACCTCAACAT GTACCACTCT 
CCCCCGATCG AGAACCCCTA TTCTTCGGCC GGACTCGACA CTATTCGCAC CGATTTTTCC 
ATGGACATGA ACATGGATAG TGGCTCCGTC AGCGCAGCCA GCGTACATCC CACCCCTGGG 
CTTAATAAGC AGGACGACGA GATGATGACC ATGGAGCAGG GCTTTGGTGG AGGTGACGAT 
GCCAACGCCT CTCACCAGGC CCAACAAAAC ATGGGCGGTT TGACTCCGGC CATGACTCCG 
GCCATGACCC CTGCAATGAC CCCGGGTGTC AGCAACTTTG CACAAGGCAT GGCTACACCA 
GTCTCACAGG ATGCGGCAAG CACACCCGCG ACAACCTTCC AGTCTCCGTC CTTATCCGCA 
ACAACACAGA CAATACGAAT TGGGCCGCCG CCACCGCCGA GCGTCACCAA TGCCCCCACC 
CCAGCGCCAT TTACCTCGAC GCCGTCAGGT GGAGGAGCGT CCCAGACAAA GAGCATTTAC 
TCCAAAAGCG GTTTCGACAT GCTGAGGGCG TTGTGGTATG TGGCATCGAG GAAGGACCCA 
AAGCTTAAGC TCGGTGCCGT GGACATGTCT TGTGCATTTG TCGTCTGTGA TGTAACACTC 
AACGATTGCC CCATCATTTA TGTGTCCGAT AACTTCCAAA ACCTCACGGG CTATAGTCGC 
CACGAGATTG TCGGGCGGAA CTGCCGCTTC TTGCAGGCCC CCGACGGTAA TGTGGAGGCT 
GGCACCAAGC GCGAATTTGT CGAAAACAAC GCCGTATATA CGCTCAAGAA GACCATCGCG 
GAGGGCCAGG AGATTCAGCA GAGTCTGATC AATTATCGGA AGGGCGGCAA GCCTTTCCTG 
AATTTGTTGA CCATGATTCC CATTCCCTGG GATACCGAAG AAATCCGTTA TTTCATTGGA 
TTTCAGATCG ATCTCGTGGA ATGCCCTGAT GCCATAATCG GCCAGGAAGG AAATGGGCCC 
ATGCAGGTCA ACTATACCCA CAGCGATATC GGGCAGTACA TCTGGACGCC ACCTACCCAG 
AAGCAACTGG AGCCTGCCGA CGGCCAGACT TTGGGCGTTG ATGATGTTTC GACACTTTTG 
CAGCAATGTA ATTCGAAGGG TGTTGCATCC GACTGGCACA AACAATCCTG GGATAAGATG 
CTTTTGGAGA ACGCAGACGA TGTTGTCCAT GTGTTGTCGC TCAAGGGTCT CTTCTTATAC 
CTATCTCCGG CCTGTAAGAA AGTGTTGGAG TACGATGCCA GCGACCTCGT TGGGACCTCG 
CTGTCCTCGA TTTGCCACCC GTCCGACATC GTGCCGGTGA CGAGGGAGTT GAAAGAGGCC 
CAGCAACACA CTCCAGTCAA TATAGTCTTC AGAATTCGGA GAAAGAACAG TGGTTATACC 
TGGTTTGAAA GCCACGGAAC ATTGTTCAAC GAACAGGGTA AGGGTCGCAA GTGTATCATT 
CTTGTGGGGA GAAAACGCCC CGTTTTTGCT CTACACAGAA AGGACCTCGA ACTCAACGGC 
GGTATTGGTG ACAGCGAAAT TTGGACCAAG GTCTCGACAT CAGGCATGTT CTTGTTCGTT 
TCGTCGAATG TCCGGTCGCT CCTGGATTTG CTCCCGGAGA ACCTCCAAGG TACCAGCATG 
CAAGATCTCA TGCGCAAGGA ATCCAGAGCC GAGTTTGGGA GGACGATAGA GAAAGCTAGA 
AAGGGCAAGA TCGCGAGCTG CAAGCATGAG GTGCAAAACA AGCGTGGCCA GGTGCTCCAG 
GCCTATACAA CATTCTACCC CGGAGATGGT GGTGAGGGTC AAAGGCCAAC GTTTTTGTTG 
GCGCAAACAA AGCTGTTGAA GGCGTCATCA AGGACACTAG CTCCCGCGAC CGTAACGGTC 
AAGAATATGT CTCCGGGCGG TGTTCCTTTG TCGCCAATGA AAGGGATCCA GACCGACAGT 
GATAGCAACA CTCTCATGGG CGGGATGTCG AAGTCGGGAA GCAGCGATAG TACGGGGGCC 
ATGGTCTCAG CTCGTAGCAG TGCAGGACCC GGCCAGGATG CGGCTTTGGA CGCCGATAAC 
ATATTTGACG AACTCAAGAC AACGCGTTGC ACCAGCTGGC AGTACGAGCT GAGGCAAATG 
GAGAAGGTGA ATCGCATGTT GGCCGAAGAG CTGGCGCAGC TTTTGTCTAA CAAGAAAAAA 
CGGAAACGAA GAAAGGGAGG AGGTAACATG GTGAGGGATT GTGCAAATTG CCATACGAGG 
AACACGCCCG AATGGCGGCG TGGGCCAAGC GGGAATCGAG ATCTATGCAA CAGTTGTGGG 
TTGAGATGGG CAAAGCAGAC TGGGCGCGTA TCGCCGCGCA CCTCATCCCG AGGTGGGAAT 
GGTGATTCGA TGAGCAAGAA GAGCAATTCA CCTAGTCACT CGTCGCCCCT TCATCGAGAA 
GTCGGCAACG ATTCACCGTC TACTACCACC GCTACCAAGA ACTCACCCTC TCTTCGAGGA 
AGTAGCACCA CTGCACCCGG TACGATCACA ACGGACAGCG GACCAGCAGT CGCATCGAGC 
GCTAGTGGAA CCGGTAGTAC GACTATAGCT ACTTCAGCCA ATTCTGCTGC ATCAACCGTC 
AACGCCCTGG GCCCTCCTGC GACAGGTCCC AGTGGCGGCT CACCGGCTCA ACATCTTCCG 
CCTCATCTCC AGGGAACACA CCTTAACGCC CAGGCCATGC AGCGTGTACA CCAGCATAAG 
CAGCATCAGC AGCATCAACA GCAGCATCAA CAGCAGCATC AACAGCAGCA TCAACAGCAG 
CACCAACAGC TTCAGCAGCA TCAGTTCAAC CCTCCACAAA GCCAGCCACT CCTGGAAGGC 
GGAAGCGGTT TCCGTGGAAG CGGAATGGAG ATGACCTCAA TTAGGGAAGA GATGGGCGAA 
CATCAACAGG GCTTAAGTGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wc-1 ( XM_011396848.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011395150.1 Neurospora crassa OR74A white collar 1 protein, variant 2 (wc-1), mRNA.

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CloneID	ONf11183
Clone ID Related Accession (Same CDS sequence)	XM_011396849.1 , XM_011396851.1 , XM_011396850.1 , XM_011396848.1
Accession Version	XM_011396848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	white collar 1 protein, variant 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507).

ATGAACAACA ACTACTACGG TTCCCCGCTG TCTCCTGAGG AGCTTCAGCA TCAAATGCAC 
CAGCACCAGC AGCAGCAGCA GCAACAACAA CAACAACAAC AGCAGCAGCA GCAGCAGCAA 
CAACAACAAC AGCAACAACA GCAACAACAG CATCAGCATC AGCAGCAACA AAAAACCAAT 
CAGCATCGCA ATGCCGGCAT GATGAATACG CCTCCAACTA CAAATCAAGG AAACAGCACG 
ATTCACGCTT CAGATGTAAC CATGTCAGGA GGAAGCGACT CTCTTGATGA AATCATCCAG 
CAGAACCTCG ACGAAATGCA TCGAAGGCGG AGTGTCCCCC AGCCCTATGG GGGCCAGACT 
AGGAGGTTGT CCATGTTTGA TTACGCGAAC CCCAACGATG GTTTCTCGGA CTATCAACTC 
GATAATATGT CAGGAAATTA CGGGGACATG ACTGGCGGCA TGGGCATGTC AGGGCACTCG 
AGCCCGTATG CAGGGCAGAA CATCATGGCC ATGTCGGACC ACAGCGGTGG ATACTCGCAC 
ATGTCGCCCA ATGTTATGGG CAACATGATG ACTTACCCCA ACCTCAACAT GTACCACTCT 
CCCCCGATCG AGAACCCCTA TTCTTCGGCC GGACTCGACA CTATTCGCAC CGATTTTTCC 
ATGGACATGA ACATGGATAG TGGCTCCGTC AGCGCAGCCA GCGTACATCC CACCCCTGGG 
CTTAATAAGC AGGACGACGA GATGATGACC ATGGAGCAGG GCTTTGGTGG AGGTGACGAT 
GCCAACGCCT CTCACCAGGC CCAACAAAAC ATGGGCGGTT TGACTCCGGC CATGACTCCG 
GCCATGACCC CTGCAATGAC CCCGGGTGTC AGCAACTTTG CACAAGGCAT GGCTACACCA 
GTCTCACAGG ATGCGGCAAG CACACCCGCG ACAACCTTCC AGTCTCCGTC CTTATCCGCA 
ACAACACAGA CAATACGAAT TGGGCCGCCG CCACCGCCGA GCGTCACCAA TGCCCCCACC 
CCAGCGCCAT TTACCTCGAC GCCGTCAGGT GGAGGAGCGT CCCAGACAAA GAGCATTTAC 
TCCAAAAGCG GTTTCGACAT GCTGAGGGCG TTGTGGTATG TGGCATCGAG GAAGGACCCA 
AAGCTTAAGC TCGGTGCCGT GGACATGTCT TGTGCATTTG TCGTCTGTGA TGTAACACTC 
AACGATTGCC CCATCATTTA TGTGTCCGAT AACTTCCAAA ACCTCACGGG CTATAGTCGC 
CACGAGATTG TCGGGCGGAA CTGCCGCTTC TTGCAGGCCC CCGACGGTAA TGTGGAGGCT 
GGCACCAAGC GCGAATTTGT CGAAAACAAC GCCGTATATA CGCTCAAGAA GACCATCGCG 
GAGGGCCAGG AGATTCAGCA GAGTCTGATC AATTATCGGA AGGGCGGCAA GCCTTTCCTG 
AATTTGTTGA CCATGATTCC CATTCCCTGG GATACCGAAG AAATCCGTTA TTTCATTGGA 
TTTCAGATCG ATCTCGTGGA ATGCCCTGAT GCCATAATCG GCCAGGAAGG AAATGGGCCC 
ATGCAGGTCA ACTATACCCA CAGCGATATC GGGCAGTACA TCTGGACGCC ACCTACCCAG 
AAGCAACTGG AGCCTGCCGA CGGCCAGACT TTGGGCGTTG ATGATGTTTC GACACTTTTG 
CAGCAATGTA ATTCGAAGGG TGTTGCATCC GACTGGCACA AACAATCCTG GGATAAGATG 
CTTTTGGAGA ACGCAGACGA TGTTGTCCAT GTGTTGTCGC TCAAGGGTCT CTTCTTATAC 
CTATCTCCGG CCTGTAAGAA AGTGTTGGAG TACGATGCCA GCGACCTCGT TGGGACCTCG 
CTGTCCTCGA TTTGCCACCC GTCCGACATC GTGCCGGTGA CGAGGGAGTT GAAAGAGGCC 
CAGCAACACA CTCCAGTCAA TATAGTCTTC AGAATTCGGA GAAAGAACAG TGGTTATACC 
TGGTTTGAAA GCCACGGAAC ATTGTTCAAC GAACAGGGTA AGGGTCGCAA GTGTATCATT 
CTTGTGGGGA GAAAACGCCC CGTTTTTGCT CTACACAGAA AGGACCTCGA ACTCAACGGC 
GGTATTGGTG ACAGCGAAAT TTGGACCAAG GTCTCGACAT CAGGCATGTT CTTGTTCGTT 
TCGTCGAATG TCCGGTCGCT CCTGGATTTG CTCCCGGAGA ACCTCCAAGG TACCAGCATG 
CAAGATCTCA TGCGCAAGGA ATCCAGAGCC GAGTTTGGGA GGACGATAGA GAAAGCTAGA 
AAGGGCAAGA TCGCGAGCTG CAAGCATGAG GTGCAAAACA AGCGTGGCCA GGTGCTCCAG 
GCCTATACAA CATTCTACCC CGGAGATGGT GGTGAGGGTC AAAGGCCAAC GTTTTTGTTG 
GCGCAAACAA AGCTGTTGAA GGCGTCATCA AGGACACTAG CTCCCGCGAC CGTAACGGTC 
AAGAATATGT CTCCGGGCGG TGTTCCTTTG TCGCCAATGA AAGGGATCCA GACCGACAGT 
GATAGCAACA CTCTCATGGG CGGGATGTCG AAGTCGGGAA GCAGCGATAG TACGGGGGCC 
ATGGTCTCAG CTCGTAGCAG TGCAGGACCC GGCCAGGATG CGGCTTTGGA CGCCGATAAC 
ATATTTGACG AACTCAAGAC AACGCGTTGC ACCAGCTGGC AGTACGAGCT GAGGCAAATG 
GAGAAGGTGA ATCGCATGTT GGCCGAAGAG CTGGCGCAGC TTTTGTCTAA CAAGAAAAAA 
CGGAAACGAA GAAAGGGAGG AGGTAACATG GTGAGGGATT GTGCAAATTG CCATACGAGG 
AACACGCCCG AATGGCGGCG TGGGCCAAGC GGGAATCGAG ATCTATGCAA CAGTTGTGGG 
TTGAGATGGG CAAAGCAGAC TGGGCGCGTA TCGCCGCGCA CCTCATCCCG AGGTGGGAAT 
GGTGATTCGA TGAGCAAGAA GAGCAATTCA CCTAGTCACT CGTCGCCCCT TCATCGAGAA 
GTCGGCAACG ATTCACCGTC TACTACCACC GCTACCAAGA ACTCACCCTC TCTTCGAGGA 
AGTAGCACCA CTGCACCCGG TACGATCACA ACGGACAGCG GACCAGCAGT CGCATCGAGC 
GCTAGTGGAA CCGGTAGTAC GACTATAGCT ACTTCAGCCA ATTCTGCTGC ATCAACCGTC 
AACGCCCTGG GCCCTCCTGC GACAGGTCCC AGTGGCGGCT CACCGGCTCA ACATCTTCCG 
CCTCATCTCC AGGGAACACA CCTTAACGCC CAGGCCATGC AGCGTGTACA CCAGCATAAG 
CAGCATCAGC AGCATCAACA GCAGCATCAA CAGCAGCATC AACAGCAGCA TCAACAGCAG 
CACCAACAGC TTCAGCAGCA TCAGTTCAAC CCTCCACAAA GCCAGCCACT CCTGGAAGGC 
GGAAGCGGTT TCCGTGGAAG CGGAATGGAG ATGACCTCAA TTAGGGAAGA GATGGGCGAA 
CATCAACAGG GCTTAAGTGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011395150.1
CDS	470..3973
Translation	MNNNYYGSPLSPEELQHQMHQHQQQQQQQQQQQQQQQQQQQQQQQQQQQQHQHQQQQKTNQHRNAGMMNTPPTTNQGNSTIHASDVTMSGGSDSLDEIIQQNLDEMHRRRSVPQPYGGQTRRLSMFDYANPNDGFSDYQLDNMSGNYGDMTGGMGMSGHSSPYAGQNIMAMSDHSGGYSHMSPNVMGNMMTYPNLNMYHSPPIENPYSSAGLDTIRTDFSMDMNMDSGSVSAASVHPTPGLNKQDDEMMTMEQGFGGGDDANASHQAQQNMGGLTPAMTPAMTPAMTPGVSNFAQGMATPVSQDAASTPATTFQSPSLSATTQTIRIGPPPPPSVTNAPTPAPFTSTPSGGGASQTKSIYSKSGFDMLRALWYVASRKDPKLKLGAVDMSCAFVVCDVTLNDCPIIYVSDNFQNLTGYSRHEIVGRNCRFLQAPDGNVEAGTKREFVENNAVYTLKKTIAEGQEIQQSLINYRKGGKPFLNLLTMIPIPWDTEEIRYFIGFQIDLVECPDAIIGQEGNGPMQVNYTHSDIGQYIWTPPTQKQLEPADGQTLGVDDVSTLLQQCNSKGVASDWHKQSWDKMLLENADDVVHVLSLKGLFLYLSPACKKVLEYDASDLVGTSLSSICHPSDIVPVTRELKEAQQHTPVNIVFRIRRKNSGYTWFESHGTLFNEQGKGRKCIILVGRKRPVFALHRKDLELNGGIGDSEIWTKVSTSGMFLFVSSNVRSLLDLLPENLQGTSMQDLMRKESRAEFGRTIEKARKGKIASCKHEVQNKRGQVLQAYTTFYPGDGGEGQRPTFLLAQTKLLKASSRTLAPATVTVKNMSPGGVPLSPMKGIQTDSDSNTLMGGMSKSGSSDSTGAMVSARSSAGPGQDAALDADNIFDELKTTRCTSWQYELRQMEKVNRMLAEELAQLLSNKKKRKRRKGGGNMVRDCANCHTRNTPEWRRGPSGNRDLCNSCGLRWAKQTGRVSPRTSSRGGNGDSMSKKSNSPSHSSPLHREVGNDSPSTTTATKNSPSLRGSSTTAPGTITTDSGPAVASSASGTGSTTIATSANSAASTVNALGPPATGPSGGSPAQHLPPHLQGTHLNAQAMQRVHQHKQHQQHQQQHQQQHQQQHQQQHQQLQQHQFNPPQSQPLLEGGSGFRGSGMEMTSIREEMGEHQQGLSV

Target ORF information:

RefSeq Version	XM_011396848.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A white collar 1 protein, variant 2 (wc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396848.1

ORF Insert Sequence:

ATGAACAACA ACTACTACGG TTCCCCGCTG TCTCCTGAGG AGCTTCAGCA TCAAATGCAC 
CAGCACCAGC AGCAGCAGCA GCAACAACAA CAACAACAAC AGCAGCAGCA GCAGCAGCAA 
CAACAACAAC AGCAACAACA GCAACAACAG CATCAGCATC AGCAGCAACA AAAAACCAAT 
CAGCATCGCA ATGCCGGCAT GATGAATACG CCTCCAACTA CAAATCAAGG AAACAGCACG 
ATTCACGCTT CAGATGTAAC CATGTCAGGA GGAAGCGACT CTCTTGATGA AATCATCCAG 
CAGAACCTCG ACGAAATGCA TCGAAGGCGG AGTGTCCCCC AGCCCTATGG GGGCCAGACT 
AGGAGGTTGT CCATGTTTGA TTACGCGAAC CCCAACGATG GTTTCTCGGA CTATCAACTC 
GATAATATGT CAGGAAATTA CGGGGACATG ACTGGCGGCA TGGGCATGTC AGGGCACTCG 
AGCCCGTATG CAGGGCAGAA CATCATGGCC ATGTCGGACC ACAGCGGTGG ATACTCGCAC 
ATGTCGCCCA ATGTTATGGG CAACATGATG ACTTACCCCA ACCTCAACAT GTACCACTCT 
CCCCCGATCG AGAACCCCTA TTCTTCGGCC GGACTCGACA CTATTCGCAC CGATTTTTCC 
ATGGACATGA ACATGGATAG TGGCTCCGTC AGCGCAGCCA GCGTACATCC CACCCCTGGG 
CTTAATAAGC AGGACGACGA GATGATGACC ATGGAGCAGG GCTTTGGTGG AGGTGACGAT 
GCCAACGCCT CTCACCAGGC CCAACAAAAC ATGGGCGGTT TGACTCCGGC CATGACTCCG 
GCCATGACCC CTGCAATGAC CCCGGGTGTC AGCAACTTTG CACAAGGCAT GGCTACACCA 
GTCTCACAGG ATGCGGCAAG CACACCCGCG ACAACCTTCC AGTCTCCGTC CTTATCCGCA 
ACAACACAGA CAATACGAAT TGGGCCGCCG CCACCGCCGA GCGTCACCAA TGCCCCCACC 
CCAGCGCCAT TTACCTCGAC GCCGTCAGGT GGAGGAGCGT CCCAGACAAA GAGCATTTAC 
TCCAAAAGCG GTTTCGACAT GCTGAGGGCG TTGTGGTATG TGGCATCGAG GAAGGACCCA 
AAGCTTAAGC TCGGTGCCGT GGACATGTCT TGTGCATTTG TCGTCTGTGA TGTAACACTC 
AACGATTGCC CCATCATTTA TGTGTCCGAT AACTTCCAAA ACCTCACGGG CTATAGTCGC 
CACGAGATTG TCGGGCGGAA CTGCCGCTTC TTGCAGGCCC CCGACGGTAA TGTGGAGGCT 
GGCACCAAGC GCGAATTTGT CGAAAACAAC GCCGTATATA CGCTCAAGAA GACCATCGCG 
GAGGGCCAGG AGATTCAGCA GAGTCTGATC AATTATCGGA AGGGCGGCAA GCCTTTCCTG 
AATTTGTTGA CCATGATTCC CATTCCCTGG GATACCGAAG AAATCCGTTA TTTCATTGGA 
TTTCAGATCG ATCTCGTGGA ATGCCCTGAT GCCATAATCG GCCAGGAAGG AAATGGGCCC 
ATGCAGGTCA ACTATACCCA CAGCGATATC GGGCAGTACA TCTGGACGCC ACCTACCCAG 
AAGCAACTGG AGCCTGCCGA CGGCCAGACT TTGGGCGTTG ATGATGTTTC GACACTTTTG 
CAGCAATGTA ATTCGAAGGG TGTTGCATCC GACTGGCACA AACAATCCTG GGATAAGATG 
CTTTTGGAGA ACGCAGACGA TGTTGTCCAT GTGTTGTCGC TCAAGGGTCT CTTCTTATAC 
CTATCTCCGG CCTGTAAGAA AGTGTTGGAG TACGATGCCA GCGACCTCGT TGGGACCTCG 
CTGTCCTCGA TTTGCCACCC GTCCGACATC GTGCCGGTGA CGAGGGAGTT GAAAGAGGCC 
CAGCAACACA CTCCAGTCAA TATAGTCTTC AGAATTCGGA GAAAGAACAG TGGTTATACC 
TGGTTTGAAA GCCACGGAAC ATTGTTCAAC GAACAGGGTA AGGGTCGCAA GTGTATCATT 
CTTGTGGGGA GAAAACGCCC CGTTTTTGCT CTACACAGAA AGGACCTCGA ACTCAACGGC 
GGTATTGGTG ACAGCGAAAT TTGGACCAAG GTCTCGACAT CAGGCATGTT CTTGTTCGTT 
TCGTCGAATG TCCGGTCGCT CCTGGATTTG CTCCCGGAGA ACCTCCAAGG TACCAGCATG 
CAAGATCTCA TGCGCAAGGA ATCCAGAGCC GAGTTTGGGA GGACGATAGA GAAAGCTAGA 
AAGGGCAAGA TCGCGAGCTG CAAGCATGAG GTGCAAAACA AGCGTGGCCA GGTGCTCCAG 
GCCTATACAA CATTCTACCC CGGAGATGGT GGTGAGGGTC AAAGGCCAAC GTTTTTGTTG 
GCGCAAACAA AGCTGTTGAA GGCGTCATCA AGGACACTAG CTCCCGCGAC CGTAACGGTC 
AAGAATATGT CTCCGGGCGG TGTTCCTTTG TCGCCAATGA AAGGGATCCA GACCGACAGT 
GATAGCAACA CTCTCATGGG CGGGATGTCG AAGTCGGGAA GCAGCGATAG TACGGGGGCC 
ATGGTCTCAG CTCGTAGCAG TGCAGGACCC GGCCAGGATG CGGCTTTGGA CGCCGATAAC 
ATATTTGACG AACTCAAGAC AACGCGTTGC ACCAGCTGGC AGTACGAGCT GAGGCAAATG 
GAGAAGGTGA ATCGCATGTT GGCCGAAGAG CTGGCGCAGC TTTTGTCTAA CAAGAAAAAA 
CGGAAACGAA GAAAGGGAGG AGGTAACATG GTGAGGGATT GTGCAAATTG CCATACGAGG 
AACACGCCCG AATGGCGGCG TGGGCCAAGC GGGAATCGAG ATCTATGCAA CAGTTGTGGG 
TTGAGATGGG CAAAGCAGAC TGGGCGCGTA TCGCCGCGCA CCTCATCCCG AGGTGGGAAT 
GGTGATTCGA TGAGCAAGAA GAGCAATTCA CCTAGTCACT CGTCGCCCCT TCATCGAGAA 
GTCGGCAACG ATTCACCGTC TACTACCACC GCTACCAAGA ACTCACCCTC TCTTCGAGGA 
AGTAGCACCA CTGCACCCGG TACGATCACA ACGGACAGCG GACCAGCAGT CGCATCGAGC 
GCTAGTGGAA CCGGTAGTAC GACTATAGCT ACTTCAGCCA ATTCTGCTGC ATCAACCGTC 
AACGCCCTGG GCCCTCCTGC GACAGGTCCC AGTGGCGGCT CACCGGCTCA ACATCTTCCG 
CCTCATCTCC AGGGAACACA CCTTAACGCC CAGGCCATGC AGCGTGTACA CCAGCATAAG 
CAGCATCAGC AGCATCAACA GCAGCATCAA CAGCAGCATC AACAGCAGCA TCAACAGCAG 
CACCAACAGC TTCAGCAGCA TCAGTTCAAC CCTCCACAAA GCCAGCCACT CCTGGAAGGC 
GGAAGCGGTT TCCGTGGAAG CGGAATGGAG ATGACCTCAA TTAGGGAAGA GATGGGCGAA 
CATCAACAGG GCTTAAGTGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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wc-1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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