stk-12 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	stk-12
Entrez Gene ID	3875009
Full Name	serine/threonine protein kinase-12
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_953778.2	XP_958871.1	serine threonine protein kinase

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	ppk18	NP_001018270.2
Magnaporthe oryzae (rice blast fungus)	MGG_00345	XP_003718738.1
Neurospora crassa	NCU07378	XP_958871.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following stk-12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the stk-12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf00807	XM_953778.2 Latest version!	Neurospora crassa OR74A serine threonine protein kinase (stk-12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

stk-12 ( XM_953778.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_958871.1 Neurospora crassa OR74A serine threonine protein kinase (stk-12), mRNA.

Price & Availability↑

CloneID	ONf00807
Clone ID Related Accession (Same CDS sequence)	XM_953778.2
Accession Version	XM_953778.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	serine threonine protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_953778.1.

ATGGAGGAAC AATCCAAGGC CCCGAGCCTG CTGGCTCCGC CGGCCGTATC GTCGCTCAGG 
ACTGGCAAGA TGGAGCGATC AGTCAGCGAG AATATCCGCG AGGAGAGAGA GGACCTTCGA 
GAGGCCGCCG AACAGACCCT AAACGTCATC ATGGACCTCA ACCTGGACGG TACTATCCGA 
TGGGTCAGCC CTTCGTGGGT TGATGTGATT GGAACCCAAC CCGAACAAGT TACGGGAACT 
GCTATCACAG ACCTGATCGT CAGCGACAAC AAGTCCATCT TTGCCGATGT CATCGAAAGC 
ATGCAAAGGG ACGATTCAAA ATCAAAGTTC ATCCGCTTTG CTGTCAAGGT CGGCCCACTG 
TCGCGGCTAC TCCCGCTTGA GCAAACAGAG GAGCCGACAA TTATCGACGT GGAGGCACAA 
GGTATCATGG TCTATGATGG TGCATCTGGT AAAGACAGCC ATACCATGTG GATGATACGG 
CCGTGGGTAG CGCCGCGTGA GATCAAAATC GATCTACCAG CCTTTATCGT AGAATCTCTC 
GGCTCAGGAG CTGAGGTTCT CGCTAGCTAT CTTACCCAAC TTGCAGAGTC CGGACTGGAT 
GACCCAAATG GCCATCCTCC ACCAGCGCCA GTGCTCTGCC GTATTTGCGA ACGCCAGATC 
CCGCCTTGGT GGTTCGAAAA GCACACCGAT TTGTGTCTAC AGGAGCATCG AGCGGAAATG 
GATGTTCAGA TGACCCAGGA AAGTCTAATT GATCACCGTC ACGCCATTGT CAAAGTACTC 
GACGCCCTCG AGGCCCGTCA TAGCCGCACT TTGATTGGAG ACCCTGCCAA TTTGCCGATT 
GCCGAGTACA AAGGCATGCC CATTGGTCCT CCAACATCCA CGACATCGTC CCCGGGGACA 
TCATCGCCAA GTTCAAACTC CGGCCGGTCG AGGGACAGAT CATCTGGATT CGGACATTCG 
AGAGCCAGAT CCTTTGCTAT CCGTCGGCCG CAAGCCCGAA TTGTTGAGCT GCTCCTTGAT 
CTATGCGACA CTGCGATCGA GATTAGCACC CCTGCCATCA AGGAGACCAC ACAAAATCCG 
GCAGAATTTC GCACGCAGTC ACCCCAGTCA GAGGGCCGTA TTTCCCAGGT GATGCAATGG 
GAAACACCTG GCACGAACAC ACTCGAGCAA GAACAAGGTC TTGCGTTGCT CTGTACTGAT 
ACGGAGGCCG TTGCTAAGGC CAAGGTTGAA GCTGTCTTCC GCCATCGCAG GATTCTCGAG 
TATGCCGAGA GGATTCGAAT CGAATTTGCC ATCGTGGTCC AGGAGTGTAT CGATGAGGCT 
ATGCGTAGGG CTGCCAAGAT TGCCGCTGGT CGACTTAGTG ACTCTACTGA AGAGGAGGAA 
GAAGAGGACT TCGAGCAGAC TGAGGACGAA GAAGCGACGT CTGCACAGGA GGAGGGGATA 
TTCCCCGGGT CCTTCGATGC TCCTTCGACA CTGGCATTGG CTTTGGAAAA GGCCCACATT 
TCGGGAGAGC GCCCCAGCCG CCGATTATCA ATATCAATCA AGTCTACTCG ATCTAGCAGC 
CCAAAGGAAT GTCCCACACC TCGATCTCAT CGGGGTACAC TTGGTAACCT TGCCAGCGCA 
ACCCAATCGA GACGAGAATC CATGCTCTTG GAAAGCGACG CTGGCGATAG TGATGGCGGA 
AGCATCAGAT CCTCGTCTGT CGCCTCGAGG ATGCCAACCC GAACAGAATC GCCGCTGTCT 
GAGTTTAGCG ACCTTCGCCG GCATGCAAGT TCGCGACAAC GTAATCGAAG GAGCCTCATT 
CTTCCAGGAA CAATATCCCC TCGAAGACAA GAATCCCCGT CGCGAAATGG ACCACCATCG 
TCACCCCTCC GACTTCACAA ACCGCGTAAT TTGCCTTTCC CTTCTGACAG CCTGGTCTCG 
CCCGAAGCAT CACCGATGCT GCCGAGTAGT GAGTTTACTT CTCCGACCCC TCCCAGTATC 
CATCATCACC GGCGACAATC CTCAGCAGCC GTGTCTGCAT CAACCGGGGA TCTTGGTTTC 
AAACCCCCAC CCTCACCTCG TCTCAGCGCG GTCGTTGGCG CTACGCAAGC CAAAGCAGTT 
CCTCCATCGA TCAAGGACTT CGAGATCATC AAACCCATCA GCAAGGGTGC GTTCGGAAGC 
GTCTATCTAT CCAAGAAGAA GTCAACCGGC GAATATTTTG CGATCAAGGT CTTGAAGAAG 
GCCGACATGG TAGCCAAGAA CCAGGTCACC AATGTCAAGG CAGAACGTGC CATCATGATG 
TGGCAGGGCG AAAGCGACTT TGTCGCCAAG CTGTATTGGA CGTTTTCGAG TAAGGACTAC 
CTCTACCTGG TCATGGAATA TTTGAATGGT GGCGACTGCG CGTCATTGAT CAAAGTACTG 
GGGGGTCTCC CCGAGGACTG GGTGAAGAAG TATCTCGGTG AGGTTGTTCT CGGTGTCGAA 
CACCTCCACA GTCGGGGCAT CGTTCACCGT GACCTGAAGC CCGACAATCT GCTTATTGAC 
CAGAAGGGAC ACCTGAAGTT GACTGACTTT GGCCTCTCGC GCATGGGTCT CGTTGGTCGA 
CAGAAGCGTG CTCTTAACAA TGGCGCTGAC GCAGCTCCGG ATTTGCTCAA GCAAGGCCCT 
TTTGTTCGGT CCTCTTCGAT CGCATCATCT CGTTCCACTT CTCTCGACGT TCATGCTCGC 
AACCCATCCC CTCTGTCGAC CCCACAAATG ACGCCGAGCG ACTATGGTGC CGGCCTAGGA 
CAGCCATCCT ACTTCAACCT CGGGGTGTTG TCTCAAGAAC CTCGGCGGTT ATGCGCGAAC 
CGCAGTGACA GTGGCGGTAG CGAAGCACTG TCGCATATGC TTCCAAATCT GTCCCTGAAC 
GACACCCTGT CCGTCCCGCC TCAAGCCATT CAGTCTCCTG GCGAGCGTAG CGAAGCCGAC 
GAGGGCACAA CTCCCGACTT CGTCTCGCTC TCCCACGCAA CCACGCAAAG CAACGTAGAC 
GCCAACAGAG GTACTCCTCC ACAACAGCCT CATATGCCCC CGCCGAATTG GGCGCTTTTC 
GACCCTCAGG ATACCAATCG CCGTTTTGTT GGAACACCCG ACTACCTAGC CCCAGAAACT 
ATCAGGGGAG AGCCTCAAGA CGAGACAAGT GATTGGTGGT CCGTTGGATG CATCATGTAC 
GAGTTTTTGT ATGGAATCCC CCCCTTCCAT GCACCTGAAG CGGAACAAGT CTTTGGGAAC 
ATCCTGGCAC GCAAATTGGA GTTCCCTGAT GACAGTGATA TGGAAATCTC TTCAGAGGCC 
AAGGATCTGA TCAACAAGTT GTTGTGTTCG GATCCTCATC AGCGGCTTGG GGCGAACCGC 
GAAGACAAGT TTCAGTCAGG AGGTGAAGAA ATACGTAGCC ATCCGTGGTT CGAGGGTGTC 
AATTGGGAGA CATTGCTGCA AGACGATGCT CAGTTCGTTC CTCAACCTGA GCATCCAGAA 
GATACCGAAT ATTTTGATTC ACGTGGCGCC GTTCTCCAGT CCTTCGCTGA AGAAATGGAA 
GATCAGTCTT CACCTCCTAG TTCAGCCCCA GCACCTGATT ATCATAACAG GCCGCATGAT 
GCGTTGGCGA AGGTTAGGTC CCAGGTCAAC TCCAACATCA ACACCGTGAA ACGAGGTTTG 
ATGCCGCTTC ACATCCCCCC TCATGTCCGT GATTTGAAGA GCAGACGCTT GAGCGAGCCA 
GTTGCTGCCG ATGATTTCGG AAACTTTTCG TTCAAAAACT TGGCTGTTCT CGAGAAGGCG 
AACAAGGATG TCATACAAAG ACTACGGGCA GAGGCTGCGG AGTCTCAGAC CAAGCCCATC 
AGTCCTGGAG CATTGAGCTC TATCAGCAGT CCTGGCACGA TTGGAGCCTT GGAAGGCAGC 
CCAGTAATCG CCAACCCCGT CCAAAGGACG CTCTCTGCTG CAAAAGCATC TCAGCGTCCT 
CAGTCTCCTT CTGGTATTGG ACACTCAAAC TCTCCGAACA GAGCTTCACA ACCTTCCTCA 
CCTCTCCTTG TGTCGTTTGT GGCTGGACAA GGTGCGGAAG GTCGACGCAA GGCGTCTAGT 
AACTCATCCA GCCTTTCACA CCAATCGGGA TCATTTCAAT CTACGGTGGA AGGGACACGT 
GTTCCACCTA CTCTTCAGAA GGCGGCCACA ACGACTACTG CCTCTTCCCC GTCAAACGGG 
CGGCTCGTCA TGCCTCCTTT ACCTCTGTCT CCGCAAAGGA CTGTTCCTGC ACACACATTA 
ACATCAAGTC CTCGGAATAC TAGCGGTCCA GCAACAGGTC GTGCTCGCTC CCTGACTGTC 
GGTTCTCAAG AAGGCAGTGG AAGTCCGATA TCTGCCGATA TTCTGGCTCA CCACAAGCGC 
AGGAGTCAGG TCTTTGATAT GTCGCCATCG TCATCCGACA CCGAGGGGGA TAAGGCGAAT 
GCGCTTCTTC GGGTACAGCG CCGGCGTCAA AGCTCCAGAC GGATGTCGCA GGTTTTGGAT 
GGTCCCATGT TTCGACCGCT AGATGTCCTG ATCTGTGAAG ACCACCCGGT ATCTCGCATG 
GTGATGGAGA AGCTCTTGGA AAAGCTCCGC TGTCGGACCA TTTCGGCGTC AACAGGTCAG 
GAAGCCTGTC GATATGCTTT GAGCGACATC AAGTTCGATA TCATCTTCAT GGAGTTCAAA 
CTACCAGTAA TCAACGGCTC TGACGTTGCC CGAATGGTCC GCGAAACGAA GAACAAAAAC 
CATCATACTC CCATCGTTGC TATCACGGCG TATCTTAAGG AACTTCATGC TCCGCATTAC 
TTCGATTCGC TTATAGAGAA GCCGATTAGT TCTTCGAAGT TAAGTGAGGT CCTGAGTACT 
TTCTGCCAGT GGAAGCCCGC TTCGCCGGGT CAGGTCCAGA TGTTGTCACT GGGCCTACCG 
CCCCCTGTAC CGTCCGGACT CAGGCAAGAA AGCCTGCGTC TGGAAGACAG CCCTGTTTCT 
GCATCCGGGT CTTCAAAATA CACTGTGCAC TCTGGCGGTA GCGTCAAAGA GGAATCAACT 
TGCTCAACAT TCGGTGACTC CGAATCTGTT GCAACAGACG ACATTCCTGT AGTGGTTAGC 
CGCAAGGCCA CTGGTGACTG GAGTGAAGGT GGGCTAGGTA TCTCAGGCTC AGAGGAGGTC 
CTAGTTGCCG GCGATGATGG ATCCAAACCA CCAGTACATG CACAGTTGTT AACGCAGCAG 
TCCGCACCAG CGCAGATGGA GCACTTGGCA GGCCAAACGA AAGGTCCAGT GCCACAGCGC 
TCACTCGATA AGTTGAGAGC AAGGCGAGAG TCCATCGAGA AGCGCAGGCT TGAAGGCTGC 
ATAGACTCTG CCGATGATGA GGATGAGGAG CTTGGTGCTG GCCAGGCGAA TTCCGGGCAG 
GAACCGCACC CTGCCAAGCA CCATCGCCCT AAGTCTACAT TACCATCGTC GAAGTTGGGA 
ATTGAAATGA TGCGGGCAAA CAGCCATGAT AGCATGACTG CCGCTGACTC TGAGGGCGCC 
AGCAGCGCTA CGCTCGGGCC ACCAACAGCC CAGATTGTCA CTCCCACAAC CGACGTGCAG 
ATGGAACTTC CTCCACTGGT TGTTGATACT GCAGCGGCAA CAATGGCTCC ATACACAACT 
GTAGCAGATG ATGAAGCCGC TGCCGCCACT GTCTCTACGC CTCCTGACCT CAGGCCTGAG 
ACTGAGGGCA GGAAAATCTG CCACGTTGAG CAAACACCGC GCCAATTGTC ACTAGCAAAG 
GTGGATGAGG AGGCTGCCGA AGCAGATGAA GAGCCTACCC CTCGTCCACT TTCCCGGTCT 
GTGAATCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_958871.1
CDS	1322..7153
Translation	MEEQSKAPSLLAPPAVSSLRTGKMERSVSENIREEREDLREAAEQTLNVIMDLNLDGTIRWVSPSWVDVIGTQPEQVTGTAITDLIVSDNKSIFADVIESMQRDDSKSKFIRFAVKVGPLSRLLPLEQTEEPTIIDVEAQGIMVYDGASGKDSHTMWMIRPWVAPREIKIDLPAFIVESLGSGAEVLASYLTQLAESGLDDPNGHPPPAPVLCRICERQIPPWWFEKHTDLCLQEHRAEMDVQMTQESLIDHRHAIVKVLDALEARHSRTLIGDPANLPIAEYKGMPIGPPTSTTSSPGTSSPSSNSGRSRDRSSGFGHSRARSFAIRRPQARIVELLLDLCDTAIEISTPAIKETTQNPAEFRTQSPQSEGRISQVMQWETPGTNTLEQEQGLALLCTDTEAVAKAKVEAVFRHRRILEYAERIRIEFAIVVQECIDEAMRRAAKIAAGRLSDSTEEEEEEDFEQTEDEEATSAQEEGIFPGSFDAPSTLALALEKAHISGERPSRRLSISIKSTRSSSPKECPTPRSHRGTLGNLASATQSRRESMLLESDAGDSDGGSIRSSSVASRMPTRTESPLSEFSDLRRHASSRQRNRRSLILPGTISPRRQESPSRNGPPSSPLRLHKPRNLPFPSDSLVSPEASPMLPSSEFTSPTPPSIHHHRRQSSAAVSASTGDLGFKPPPSPRLSAVVGATQAKAVPPSIKDFEIIKPISKGAFGSVYLSKKKSTGEYFAIKVLKKADMVAKNQVTNVKAERAIMMWQGESDFVAKLYWTFSSKDYLYLVMEYLNGGDCASLIKVLGGLPEDWVKKYLGEVVLGVEHLHSRGIVHRDLKPDNLLIDQKGHLKLTDFGLSRMGLVGRQKRALNNGADAAPDLLKQGPFVRSSSIASSRSTSLDVHARNPSPLSTPQMTPSDYGAGLGQPSYFNLGVLSQEPRRLCANRSDSGGSEALSHMLPNLSLNDTLSVPPQAIQSPGERSEADEGTTPDFVSLSHATTQSNVDANRGTPPQQPHMPPPNWALFDPQDTNRRFVGTPDYLAPETIRGEPQDETSDWWSVGCIMYEFLYGIPPFHAPEAEQVFGNILARKLEFPDDSDMEISSEAKDLINKLLCSDPHQRLGANREDKFQSGGEEIRSHPWFEGVNWETLLQDDAQFVPQPEHPEDTEYFDSRGAVLQSFAEEMEDQSSPPSSAPAPDYHNRPHDALAKVRSQVNSNINTVKRGLMPLHIPPHVRDLKSRRLSEPVAADDFGNFSFKNLAVLEKANKDVIQRLRAEAAESQTKPISPGALSSISSPGTIGALEGSPVIANPVQRTLSAAKASQRPQSPSGIGHSNSPNRASQPSSPLLVSFVAGQGAEGRRKASSNSSSLSHQSGSFQSTVEGTRVPPTLQKAATTTTASSPSNGRLVMPPLPLSPQRTVPAHTLTSSPRNTSGPATGRARSLTVGSQEGSGSPISADILAHHKRRSQVFDMSPSSSDTEGDKANALLRVQRRRQSSRRMSQVLDGPMFRPLDVLICEDHPVSRMVMEKLLEKLRCRTISASTGQEACRYALSDIKFDIIFMEFKLPVINGSDVARMVRETKNKNHHTPIVAITAYLKELHAPHYFDSLIEKPISSSKLSEVLSTFCQWKPASPGQVQMLSLGLPPPVPSGLRQESLRLEDSPVSASGSSKYTVHSGGSVKEESTCSTFGDSESVATDDIPVVVSRKATGDWSEGGLGISGSEEVLVAGDDGSKPPVHAQLLTQQSAPAQMEHLAGQTKGPVPQRSLDKLRARRESIEKRRLEGCIDSADDEDEELGAGQANSGQEPHPAKHHRPKSTLPSSKLGIEMMRANSHDSMTAADSEGASSATLGPPTAQIVTPTTDVQMELPPLVVDTAAATMAPYTTVADDEAAAATVSTPPDLRPETEGRKICHVEQTPRQLSLAKVDEEAAEADEEPTPRPLSRSVNQ

Target ORF information:

RefSeq Version	XM_953778.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A serine threonine protein kinase (stk-12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_953778.2

ORF Insert Sequence:

ATGGAGGAAC AATCCAAGGC CCCGAGCCTG CTGGCTCCGC CGGCCGTATC GTCGCTCAGG 
ACTGGCAAGA TGGAGCGATC AGTCAGCGAG AATATCCGCG AGGAGAGAGA GGACCTTCGA 
GAGGCCGCCG AACAGACCCT AAACGTCATC ATGGACCTCA ACCTGGACGG TACTATCCGA 
TGGGTCAGCC CTTCGTGGGT TGATGTGATT GGAACCCAAC CCGAACAAGT TACGGGAACT 
GCTATCACAG ACCTGATCGT CAGCGACAAC AAGTCCATCT TTGCCGATGT CATCGAAAGC 
ATGCAAAGGG ACGATTCAAA ATCAAAGTTC ATCCGCTTTG CTGTCAAGGT CGGCCCACTG 
TCGCGGCTAC TCCCGCTTGA GCAAACAGAG GAGCCGACAA TTATCGACGT GGAGGCACAA 
GGTATCATGG TCTATGATGG TGCATCTGGT AAAGACAGCC ATACCATGTG GATGATACGG 
CCGTGGGTAG CGCCGCGTGA GATCAAAATC GATCTACCAG CCTTTATCGT AGAATCTCTC 
GGCTCAGGAG CTGAGGTTCT CGCTAGCTAT CTTACCCAAC TTGCAGAGTC CGGACTGGAT 
GACCCAAATG GCCATCCTCC ACCAGCGCCA GTGCTCTGCC GTATTTGCGA ACGCCAGATC 
CCGCCTTGGT GGTTCGAAAA GCACACCGAT TTGTGTCTAC AGGAGCATCG AGCGGAAATG 
GATGTTCAGA TGACCCAGGA AAGTCTAATT GATCACCGTC ACGCCATTGT CAAAGTACTC 
GACGCCCTCG AGGCCCGTCA TAGCCGCACT TTGATTGGAG ACCCTGCCAA TTTGCCGATT 
GCCGAGTACA AAGGCATGCC CATTGGTCCT CCAACATCCA CGACATCGTC CCCGGGGACA 
TCATCGCCAA GTTCAAACTC CGGCCGGTCG AGGGACAGAT CATCTGGATT CGGACATTCG 
AGAGCCAGAT CCTTTGCTAT CCGTCGGCCG CAAGCCCGAA TTGTTGAGCT GCTCCTTGAT 
CTATGCGACA CTGCGATCGA GATTAGCACC CCTGCCATCA AGGAGACCAC ACAAAATCCG 
GCAGAATTTC GCACGCAGTC ACCCCAGTCA GAGGGCCGTA TTTCCCAGGT GATGCAATGG 
GAAACACCTG GCACGAACAC ACTCGAGCAA GAACAAGGTC TTGCGTTGCT CTGTACTGAT 
ACGGAGGCCG TTGCTAAGGC CAAGGTTGAA GCTGTCTTCC GCCATCGCAG GATTCTCGAG 
TATGCCGAGA GGATTCGAAT CGAATTTGCC ATCGTGGTCC AGGAGTGTAT CGATGAGGCT 
ATGCGTAGGG CTGCCAAGAT TGCCGCTGGT CGACTTAGTG ACTCTACTGA AGAGGAGGAA 
GAAGAGGACT TCGAGCAGAC TGAGGACGAA GAAGCGACGT CTGCACAGGA GGAGGGGATA 
TTCCCCGGGT CCTTCGATGC TCCTTCGACA CTGGCATTGG CTTTGGAAAA GGCCCACATT 
TCGGGAGAGC GCCCCAGCCG CCGATTATCA ATATCAATCA AGTCTACTCG ATCTAGCAGC 
CCAAAGGAAT GTCCCACACC TCGATCTCAT CGGGGTACAC TTGGTAACCT TGCCAGCGCA 
ACCCAATCGA GACGAGAATC CATGCTCTTG GAAAGCGACG CTGGCGATAG TGATGGCGGA 
AGCATCAGAT CCTCGTCTGT CGCCTCGAGG ATGCCAACCC GAACAGAATC GCCGCTGTCT 
GAGTTTAGCG ACCTTCGCCG GCATGCAAGT TCGCGACAAC GTAATCGAAG GAGCCTCATT 
CTTCCAGGAA CAATATCCCC TCGAAGACAA GAATCCCCGT CGCGAAATGG ACCACCATCG 
TCACCCCTCC GACTTCACAA ACCGCGTAAT TTGCCTTTCC CTTCTGACAG CCTGGTCTCG 
CCCGAAGCAT CACCGATGCT GCCGAGTAGT GAGTTTACTT CTCCGACCCC TCCCAGTATC 
CATCATCACC GGCGACAATC CTCAGCAGCC GTGTCTGCAT CAACCGGGGA TCTTGGTTTC 
AAACCCCCAC CCTCACCTCG TCTCAGCGCG GTCGTTGGCG CTACGCAAGC CAAAGCAGTT 
CCTCCATCGA TCAAGGACTT CGAGATCATC AAACCCATCA GCAAGGGTGC GTTCGGAAGC 
GTCTATCTAT CCAAGAAGAA GTCAACCGGC GAATATTTTG CGATCAAGGT CTTGAAGAAG 
GCCGACATGG TAGCCAAGAA CCAGGTCACC AATGTCAAGG CAGAACGTGC CATCATGATG 
TGGCAGGGCG AAAGCGACTT TGTCGCCAAG CTGTATTGGA CGTTTTCGAG TAAGGACTAC 
CTCTACCTGG TCATGGAATA TTTGAATGGT GGCGACTGCG CGTCATTGAT CAAAGTACTG 
GGGGGTCTCC CCGAGGACTG GGTGAAGAAG TATCTCGGTG AGGTTGTTCT CGGTGTCGAA 
CACCTCCACA GTCGGGGCAT CGTTCACCGT GACCTGAAGC CCGACAATCT GCTTATTGAC 
CAGAAGGGAC ACCTGAAGTT GACTGACTTT GGCCTCTCGC GCATGGGTCT CGTTGGTCGA 
CAGAAGCGTG CTCTTAACAA TGGCGCTGAC GCAGCTCCGG ATTTGCTCAA GCAAGGCCCT 
TTTGTTCGGT CCTCTTCGAT CGCATCATCT CGTTCCACTT CTCTCGACGT TCATGCTCGC 
AACCCATCCC CTCTGTCGAC CCCACAAATG ACGCCGAGCG ACTATGGTGC CGGCCTAGGA 
CAGCCATCCT ACTTCAACCT CGGGGTGTTG TCTCAAGAAC CTCGGCGGTT ATGCGCGAAC 
CGCAGTGACA GTGGCGGTAG CGAAGCACTG TCGCATATGC TTCCAAATCT GTCCCTGAAC 
GACACCCTGT CCGTCCCGCC TCAAGCCATT CAGTCTCCTG GCGAGCGTAG CGAAGCCGAC 
GAGGGCACAA CTCCCGACTT CGTCTCGCTC TCCCACGCAA CCACGCAAAG CAACGTAGAC 
GCCAACAGAG GTACTCCTCC ACAACAGCCT CATATGCCCC CGCCGAATTG GGCGCTTTTC 
GACCCTCAGG ATACCAATCG CCGTTTTGTT GGAACACCCG ACTACCTAGC CCCAGAAACT 
ATCAGGGGAG AGCCTCAAGA CGAGACAAGT GATTGGTGGT CCGTTGGATG CATCATGTAC 
GAGTTTTTGT ATGGAATCCC CCCCTTCCAT GCACCTGAAG CGGAACAAGT CTTTGGGAAC 
ATCCTGGCAC GCAAATTGGA GTTCCCTGAT GACAGTGATA TGGAAATCTC TTCAGAGGCC 
AAGGATCTGA TCAACAAGTT GTTGTGTTCG GATCCTCATC AGCGGCTTGG GGCGAACCGC 
GAAGACAAGT TTCAGTCAGG AGGTGAAGAA ATACGTAGCC ATCCGTGGTT CGAGGGTGTC 
AATTGGGAGA CATTGCTGCA AGACGATGCT CAGTTCGTTC CTCAACCTGA GCATCCAGAA 
GATACCGAAT ATTTTGATTC ACGTGGCGCC GTTCTCCAGT CCTTCGCTGA AGAAATGGAA 
GATCAGTCTT CACCTCCTAG TTCAGCCCCA GCACCTGATT ATCATAACAG GCCGCATGAT 
GCGTTGGCGA AGGTTAGGTC CCAGGTCAAC TCCAACATCA ACACCGTGAA ACGAGGTTTG 
ATGCCGCTTC ACATCCCCCC TCATGTCCGT GATTTGAAGA GCAGACGCTT GAGCGAGCCA 
GTTGCTGCCG ATGATTTCGG AAACTTTTCG TTCAAAAACT TGGCTGTTCT CGAGAAGGCG 
AACAAGGATG TCATACAAAG ACTACGGGCA GAGGCTGCGG AGTCTCAGAC CAAGCCCATC 
AGTCCTGGAG CATTGAGCTC TATCAGCAGT CCTGGCACGA TTGGAGCCTT GGAAGGCAGC 
CCAGTAATCG CCAACCCCGT CCAAAGGACG CTCTCTGCTG CAAAAGCATC TCAGCGTCCT 
CAGTCTCCTT CTGGTATTGG ACACTCAAAC TCTCCGAACA GAGCTTCACA ACCTTCCTCA 
CCTCTCCTTG TGTCGTTTGT GGCTGGACAA GGTGCGGAAG GTCGACGCAA GGCGTCTAGT 
AACTCATCCA GCCTTTCACA CCAATCGGGA TCATTTCAAT CTACGGTGGA AGGGACACGT 
GTTCCACCTA CTCTTCAGAA GGCGGCCACA ACGACTACTG CCTCTTCCCC GTCAAACGGG 
CGGCTCGTCA TGCCTCCTTT ACCTCTGTCT CCGCAAAGGA CTGTTCCTGC ACACACATTA 
ACATCAAGTC CTCGGAATAC TAGCGGTCCA GCAACAGGTC GTGCTCGCTC CCTGACTGTC 
GGTTCTCAAG AAGGCAGTGG AAGTCCGATA TCTGCCGATA TTCTGGCTCA CCACAAGCGC 
AGGAGTCAGG TCTTTGATAT GTCGCCATCG TCATCCGACA CCGAGGGGGA TAAGGCGAAT 
GCGCTTCTTC GGGTACAGCG CCGGCGTCAA AGCTCCAGAC GGATGTCGCA GGTTTTGGAT 
GGTCCCATGT TTCGACCGCT AGATGTCCTG ATCTGTGAAG ACCACCCGGT ATCTCGCATG 
GTGATGGAGA AGCTCTTGGA AAAGCTCCGC TGTCGGACCA TTTCGGCGTC AACAGGTCAG 
GAAGCCTGTC GATATGCTTT GAGCGACATC AAGTTCGATA TCATCTTCAT GGAGTTCAAA 
CTACCAGTAA TCAACGGCTC TGACGTTGCC CGAATGGTCC GCGAAACGAA GAACAAAAAC 
CATCATACTC CCATCGTTGC TATCACGGCG TATCTTAAGG AACTTCATGC TCCGCATTAC 
TTCGATTCGC TTATAGAGAA GCCGATTAGT TCTTCGAAGT TAAGTGAGGT CCTGAGTACT 
TTCTGCCAGT GGAAGCCCGC TTCGCCGGGT CAGGTCCAGA TGTTGTCACT GGGCCTACCG 
CCCCCTGTAC CGTCCGGACT CAGGCAAGAA AGCCTGCGTC TGGAAGACAG CCCTGTTTCT 
GCATCCGGGT CTTCAAAATA CACTGTGCAC TCTGGCGGTA GCGTCAAAGA GGAATCAACT 
TGCTCAACAT TCGGTGACTC CGAATCTGTT GCAACAGACG ACATTCCTGT AGTGGTTAGC 
CGCAAGGCCA CTGGTGACTG GAGTGAAGGT GGGCTAGGTA TCTCAGGCTC AGAGGAGGTC 
CTAGTTGCCG GCGATGATGG ATCCAAACCA CCAGTACATG CACAGTTGTT AACGCAGCAG 
TCCGCACCAG CGCAGATGGA GCACTTGGCA GGCCAAACGA AAGGTCCAGT GCCACAGCGC 
TCACTCGATA AGTTGAGAGC AAGGCGAGAG TCCATCGAGA AGCGCAGGCT TGAAGGCTGC 
ATAGACTCTG CCGATGATGA GGATGAGGAG CTTGGTGCTG GCCAGGCGAA TTCCGGGCAG 
GAACCGCACC CTGCCAAGCA CCATCGCCCT AAGTCTACAT TACCATCGTC GAAGTTGGGA 
ATTGAAATGA TGCGGGCAAA CAGCCATGAT AGCATGACTG CCGCTGACTC TGAGGGCGCC 
AGCAGCGCTA CGCTCGGGCC ACCAACAGCC CAGATTGTCA CTCCCACAAC CGACGTGCAG 
ATGGAACTTC CTCCACTGGT TGTTGATACT GCAGCGGCAA CAATGGCTCC ATACACAACT 
GTAGCAGATG ATGAAGCCGC TGCCGCCACT GTCTCTACGC CTCCTGACCT CAGGCCTGAG 
ACTGAGGGCA GGAAAATCTG CCACGTTGAG CAAACACCGC GCCAATTGTC ACTAGCAAAG 
GTGGATGAGG AGGCTGCCGA AGCAGATGAA GAGCCTACCC CTCGTCCACT TTCCCGGTCT 
GTGAATCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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stk-12 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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