NCU08441 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU08441
Entrez Gene ID	3879551
Full Name	non-ribosomal peptide synthetase
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_958318.3	XP_963411.3	non-ribosomal peptide synthetase

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_14767	XP_003714007.1
Neurospora crassa	NCU08441	XP_963411.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU08441 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU08441 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12445	XM_958318.3 Latest version!	Neurospora crassa OR74A non-ribosomal peptide synthetase (NCU08441), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU08441 ( XM_958318.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_963411.3 Neurospora crassa OR74A non-ribosomal peptide synthetase (NCU08441), mRNA.

Price & Availability↑

CloneID	ONf12445
Clone ID Related Accession (Same CDS sequence)	XM_958318.3
Accession Version	XM_958318.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	non-ribosomal peptide synthetase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502). On Feb 23, 2015 this sequence version replaced XM_958318.2.

ATGCAGCAAG TCGTGCAAGG AGCCCTCTCT TTTCTCGGCC TCAACGGCCA GAGCAGCAAC 
GCCACCAGCC ACCTCTTCGA CACACTAGGT CTGGAGACAG TATCCAGGCC ACTATCACTA 
CCATCACAAA AGAACATCTC TTTGCTAGAC GCCTCCCTCC TAGCAGGGCT GTTGCTTCTC 
CAGAGAGAGC GAGGAGAAGA TGATCGGGTA GAAAACTTCA ACTGGGGACG GAGAACATCT 
GATGGGGCGG AAACGTCGTC ACAGTTTTCC GTCAAGGCAG TGCTGGGTTC TGAAGGACTG 
GACCAGACGG CGAGTGTGAA GAATGTACTT GAGGTGCTGA AGAAGGTCAC GGGGCAAGAT 
GTGACAGAGG AGAACAGGAC CCTGTTCTTT AACGATGGGA AAGAGGCGCC GGAGAAGATC 
AAGGAAGAGG GATATGAGAC TGCTCAGCGG CCGAACTGGA AATTCCAGCT CGAGCTCCTT 
GCCTCAGAAG ACGGCGAGCA GCATGACCTC ACCCTCCAAG CCTACTGGTT CAAGAACGTT 
CCCGACGCTC TCACCAGTTC CCAAGTCACC ATTCGCCTGA ACGCCTTTAC CGAGCTTCTT 
GGCCTCATCT TCTCCTCTCC CGAGACAACC CCCATCTCCT CCCTCCTCGC CCCTCTCCCC 
TGTGACCTCA ATCAGATCTG GGCCTTCAAC GCCGTCGTGC CCCCATTGAT CGATCGCACT 
ATGCAGGACA TCATCTCCGA GCAAGCCGCC GCCAACCCCT CCAAGCCGGC CGTCTCCTCC 
TGGGACGGCG AGTTCACCTA CGCCGAGGTG GAATCGAAGT CCGACCTTTT GGCTCGCCAC 
TTGGTCTCCC GCGGCGTCAA GCTCGGCGGC GTGGTCCCGC TCATGTTTGA GAAATCCCGC 
TGGACTATCG TCGCCCTGCT GGCCGTAATG AAAGCCGGCT CAGCATTTGC TCTCACCGAT 
CCCACTTCGC AGCCTGAAGG CCGTTTGCGC GTGATGGTCG AGCAGACCGG TGGCGACATC 
ATCGTCGCTT CTGCTTCCCA GACTGAACTC GCGGAGGTCT GTCTCCCCTC TTCTTCTGGA 
GGAAGCCAAG GACAGGTCAT CACCGTCTCT GACGACCTTT TCACCACCCT CTCTCAAACC 
CCCGAGCTCA CCTCGGTGAC GCTGCCCGTG ATCCCCACGC AAACAACACC CCTCTACATC 
CAATTCACCT CTGGCTCCAC CGGCAAACCA AAAGGCGTCG TCATCTCCCA CGCCAACTAC 
ACCTCCGGCG CCCTGCCTCG CGCCGAAGCA GTCGGTTACA AGTCTTCCTC CCGCTGCTTC 
GAGTTCGCTT CCTACGCTTT TGACGTCTCC ATCGATTGCA TGCTCTGCAC CCTCGCTGTT 
GGTGGAACCA TCTGCATTCC GTCGGACGCT GATCGTATGA ACGATCTCGG GGGCGCTATC 
CTCAAGAGCG GCGCAAACAT GGCGCACATG ACTCCTTCCG TTGCCCGAGT GCTTGATCCA 
GAAGTTATCA AGGGTCTTGA TGTCCTTGGC TTGGGCGGTG AAGCAGTCTC TGCCGCGGAT 
GCAGCTGCTT GGTCGAAGGG CAAGACATCG GTCATTGTTG CGTATGGACC GAGTGAATGC 
ACTGTTGGAT GTACCGTCAA CAACGTCTTT GCGAGAGACG AGGAGGCCAA GAAGAAGGGG 
TTCACCACCG GAAACATCGG TACCGGCGTT GGCGGGTGCG GATGGGTTGT TGATCCCAAC 
GATCATGACC GTCTCGTGCC CGTGGGAGCG GTGGGTGAAT TGCTCATGGA GGGCCCGGTC 
GTGGGACTTG GGTACCTCGG TGAAGAGGAA AAGACCAAGG AGGTGTTCAT TGAAGATCCC 
AAGTGGCTCG TTGCTGGCCA TGGCGATGCT CACCCGGGAA GAAAAGGAAG GTTGTACAAG 
ACTGGTGATT TGGTCCGGTA CGATGCCGAT GGCTCGGGGG CTTTTGTCTT TGTTGGACGC 
AAAGATGCGC AGGTCAAGCT GAGAGGCCAA AGAGTAGAGT TGGTTGAGAT TGAACACCAT 
TTGCGCGCCA AGTTGCCCAG TGGCGTGAAG ATTGCTGCCG AGGTTATCAA GCCTAGTGGT 
GGTGATCCCA CGCTGGTTGC TTTCCTGGCG GAGACTAGCT CGACGGGTAT CTCGGCCAAG 
GACGCCCTGC AGAGTGTTGT TGAGGGTGAG GTTAGCTTCT CTCCTGAACT GCAGGCTGCG 
CTGGATGGGA TTGAGGAGGT GCTGGGCATT GATCTGCCTA GGTACATGGT TCCTAATGCG 
TACATCCCCC TTCGTGAGAT GCCCTCGCTG GTGTCGGGCA AGATTGATCG CAAGACGCTG 
AGGGCTATGG GAGCTGCGAT GACGAGGGAG CAAGTCTCTG GTAGCGCAAA GAAGGCCAGA 
CAGGGTGGCG AGGGCGGTGC TCCTACTACC GAGATGGAGA TTGCTCTTCA CAAGGTCTGG 
AAGCAGCTGC TTGGTACCGA GCAGGAGATT GCCGCTGGCG ATAGCTTCTT TGCTGTTGGC 
GGTGACTCTC TCAGGGCCAT GAGACTTATT GCTGCTGCCA GAGCGGAGGG CGTCAAGCTC 
ACTGTCGCTG ATGTCTTCCG GTATCCCGTA CTCAGGGACA TGGCTGTCGT CGCCACCAAG 
GTGGACACCC AGGACGCGGG TGGCAAGGAC AACAACGAGA TTGCTCCCTT CTCTCTGCTT 
GAAAGCGATT GGGCCATCGA AGACGTCAAG GCCGAGGCCA GCAAGAACTG CAATATCGAC 
AAGGACGCCC TCGAGGACGT CTATCCCTGC ACTCCCCTCC AGGAAGCCCT CATGGCTTTG 
TCCGCCAAGG TCAAGGAAGC TTACGTTGCC CAGCGTGTGC TCAAGATGGA AGGCCAAGCC 
GCCGCTGACA AGCTCAAGGC CGCCTTCGAA GCCATCAACG CCGACTGCGC CATCCTCCGC 
ACCCGCATCA TCCACTCCCC GCGCGGCCTG ATGCAAGTCG TCGTCAAGGA GCCTCTGCAC 
TGGCACTCCG CTACCACCCT CGCCGAGTAC CTTGAAACTG ACCGCAACGA GGAAATGGAC 
CTCGGCAAGC CTCTCGTGCG CTACGGCCTC ATCCAGGACG GCGACGCCTA CCACATTGTC 
CTGACCATGC ACCACGCTTT GTACGACGGC TGGTCCATGC CCCTTGTCGT CGACCGCGTC 
AACCAGGCCT ACCAAGGTCT CGTCCCGCGC AAGCCCGCCG CGGAGTTCAA GCACTTCATC 
AACTACCTCA ACAACACCCT CGACCGCGCC GCCTGCGACG CCTACTGGAA GGACCAACTC 
GCCGGCGCCA CGGGTGTGCA GTTCCCCGCT CTCCCCTTTG AGGGGTACCA AACCCGCGCC 
GACTCCCTCT GGGAGCTCTA CGTCTCTCTC GACGGGCGCA AACCCCCTTC TTCGCCCAAC 
ACCACCATCA CCCTCGCCAC CATCGTGCGC GCTGCCTGGG CCCTGGTAGC CAGCCAGTAC 
ACAGCCGGCA ACAACGACAT TGTCTTTGGC GAGACGCTCA CGGGCCGTAA CGCGCCCATC 
GTCGGCGCAG ATGAGATCGA AGGTCCCATG ATCACCACCG TTCCTGTTCG CGTGTGTATT 
GATCGCGAGC AGACCGTCGC CGAGTACTTG CAGCAGGTGG CAGAGCAAAT GATCACGCAA 
ATCCCTTACG AACACGCAGG TCTGCAGCAC ATCCGTCGGT TGTCCGATGA CGCGCTGCAA 
GCGTGCGAGT TGAGAACGGG TATTGTGCTG CATCCTGCTG CGGGCGAGGT GGGCGTTACG 
GAGAACACGC CGGCGAATGG ACTGGTTCCT GCGGGTGATA GCGAGGCGGC GCAGGAGGCG 
TTGAAGTTTA ACACGTATGC GTTGATGCTG GTTTGCTCGT TGGAGGCGGA TGGGTTCTTG 
GTCATGGCGT CTTTTGATAG TAAGACGGTG GAGATGGATG TGGTGAAGAA GGCGTTGGAT 
CAGCTGCAGG TGGTGGTGAG GCAGTTGTGT GAAGACGGTT GTGGGGAGAA GAAGGTGGGA 
CAGTTGGAGT GTTTGACGGA GAAGGATCAC GAGGAGGTGA AGAAGTTGGC GGGTGTGAAG 
AACCTGACGG CCGAGTCTGA TCCTTTTGTC GGGGGTGTCA CTGCGGAGGA TGTGGAAGGG 
GTGTGGATTG TTGATGCTGC TGATACTGCC AGGCTGGTGC CCGTTTGTGC GGCGGGCGAG 
TTGATTGTTG AGACCAGCAA GGAACTTGAT GCGCCTGTCA CTCTTCTCGC TGAGACTCCG 
GCCTGGTACG AGGGCAACAA GAAGCTGTAC AAGACTGGCC AGCTCGCCAA GTTCAAGGTT 
GACGGCGATT CTGCCTCCCT TTGCATCCTC AAGGCTTCTG AGCCCAAGAC CGAGGTGGCC 
AAGAAGAAGG TTGCTTCCTC TGTTACTCCC TCTGCTGTTG CTGCTACCTC CGCCAAGCAA 
AAGACGCTGC GCAAGATTTG GGCCAAGCTG CTTAAGGTCA AGGAGGATAG CATCTACCTC 
GGCGATAGCT TCTTCAACCG TGGTGGTGAC TCCATCACTG CCATGAAGCT GGTGTCGGAG 
GCACGCCAGC AGGGCCTCAA GATTAGCGTC GCGCAGGTCT TTGCCAACCG CACGCTCTTC 
GACATGGCCA ATGTCATGCA AACCACCGGT ACCGTGGTTG ACGCCTCGGA GAAGGTTGAG 
ACCGAGTACA AGCCTTTCTC TCTCCTCGAG GAGAATAACA AGGCCAGCAT CATGGAGGTC 
GTCAAGCACA GTCTGGCCGA TAAGTCGTGG ACCATCGCCG ACATCCTCCC CACCCGTCCC 
CTCCAGGAGA TCGCCGTCCG CGGCACCGTC GAGCTCCCCC GTTTCTCTCT CCGCTACGAG 
TTGATGCACT TTAACGGCCA GGTCGACAAA GCCGCCCTCT TCCGCGCGTG CCACGAAGTA 
GTCGCACGCA ACGAGATCCT ACGCACCGTT TTCGTCCGCG ACGCTTCCAA CACCTGTTAC 
GGCGCCGTCA TCGACAACCT CATCACCTCC ATCGCCGAGT ACGAAATCGA CGACCACGCC 
GACGTCAAGG ACTTCGCCGC CCAAGTGTGC CGCCTCGACT CGCAAACCCG CATGCCATAC 
GGTTCCTCCT TTGTGAAATG GTTCTTCATC ACCAACGGCA CTTCCCAATG CTCGCTCGCC 
TTCCGCCTTT CGCACGCCCA ATACGACGAG ATCTGCCTCC CCATCATGCT CCACCAGTTC 
CACCAGCTCT ACCTCAACTC CCCCGAATCC TCCATTCCGC CTTCCTACCC CTTCTCCCAC 
TTCGTCGCAC ACACCCTCCG CGACTCCATC CCCGCCGCCA TTCCCTACTG GCGCAACCTC 
CTCTCCGGTT CAAGCGGGAT CACCCGCTTC CGCCCTTCCA CACCCATCAC GTCCCGGAAG 
CACTTCGCCA TTCACCGCGC CGTCGACATC TCCTCCCGGC CACGCGACGT CACCATCGCC 
ACTTTACCTT CCGCCGCCTG GGCTTTGGCT CTCGCGCGCC ACGTCAAAAG TCCCGACGTC 
CTCTTCGGCG AAGTCTCCTC TGGTCGTAGC GTCGACGTCC CAGGCATCAG GGACACCAAC 
GCCGTCACCG GTCCGACATG GCAATACGTC CCTGCTCGAA TCCAATTCGA TAACCCATCT 
AAACCTCTTC GTACAGGCCA CGATTTGTTG GTGGAGATGC AGACGCAGCA TATGGCTTCT 
TCAGCGTATG ATAGTATGGG ACTGGAAGAG ATTGTCAAGA CGTGTACGGA CTGGGAACTG 
CAAAAAGATA TGTGGTTTGA TACCGTGGTG CATCAGGATG TGGCGCATGT GGAGAAGCTG 
GAGTTTTCTT CTGTTTCTGT TTCTGGGGAT GGAAATGGGG AAGAGGAAAA GAAGACTGGC 
GCAAAGGCGG AGTTTGAAAC GATGTATTGC TTTGAGGAGC CGTTGAGGGA GTGGAAGGTG 
CAGGCTTTTA GGGACGAGAA GGGCGAGGAG ATGGTGTTGG AGATTGTGAC GATGGAGTGC 
TGGAAGGAGG AGGCGGAGGT GGTGTTGGGA GAGGTTTGTG AGGCGTTGAA GCAGTTGGTG 
GAGAGGCCTT GGGAGGGGTT GGATGTTGCG AGGCTGGAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_963411.3
CDS	250..6354
Translation	MQQVVQGALSFLGLNGQSSNATSHLFDTLGLETVSRPLSLPSQKNISLLDASLLAGLLLLQRERGEDDRVENFNWGRRTSDGAETSSQFSVKAVLGSEGLDQTASVKNVLEVLKKVTGQDVTEENRTLFFNDGKEAPEKIKEEGYETAQRPNWKFQLELLASEDGEQHDLTLQAYWFKNVPDALTSSQVTIRLNAFTELLGLIFSSPETTPISSLLAPLPCDLNQIWAFNAVVPPLIDRTMQDIISEQAAANPSKPAVSSWDGEFTYAEVESKSDLLARHLVSRGVKLGGVVPLMFEKSRWTIVALLAVMKAGSAFALTDPTSQPEGRLRVMVEQTGGDIIVASASQTELAEVCLPSSSGGSQGQVITVSDDLFTTLSQTPELTSVTLPVIPTQTTPLYIQFTSGSTGKPKGVVISHANYTSGALPRAEAVGYKSSSRCFEFASYAFDVSIDCMLCTLAVGGTICIPSDADRMNDLGGAILKSGANMAHMTPSVARVLDPEVIKGLDVLGLGGEAVSAADAAAWSKGKTSVIVAYGPSECTVGCTVNNVFARDEEAKKKGFTTGNIGTGVGGCGWVVDPNDHDRLVPVGAVGELLMEGPVVGLGYLGEEEKTKEVFIEDPKWLVAGHGDAHPGRKGRLYKTGDLVRYDADGSGAFVFVGRKDAQVKLRGQRVELVEIEHHLRAKLPSGVKIAAEVIKPSGGDPTLVAFLAETSSTGISAKDALQSVVEGEVSFSPELQAALDGIEEVLGIDLPRYMVPNAYIPLREMPSLVSGKIDRKTLRAMGAAMTREQVSGSAKKARQGGEGGAPTTEMEIALHKVWKQLLGTEQEIAAGDSFFAVGGDSLRAMRLIAAARAEGVKLTVADVFRYPVLRDMAVVATKVDTQDAGGKDNNEIAPFSLLESDWAIEDVKAEASKNCNIDKDALEDVYPCTPLQEALMALSAKVKEAYVAQRVLKMEGQAAADKLKAAFEAINADCAILRTRIIHSPRGLMQVVVKEPLHWHSATTLAEYLETDRNEEMDLGKPLVRYGLIQDGDAYHIVLTMHHALYDGWSMPLVVDRVNQAYQGLVPRKPAAEFKHFINYLNNTLDRAACDAYWKDQLAGATGVQFPALPFEGYQTRADSLWELYVSLDGRKPPSSPNTTITLATIVRAAWALVASQYTAGNNDIVFGETLTGRNAPIVGADEIEGPMITTVPVRVCIDREQTVAEYLQQVAEQMITQIPYEHAGLQHIRRLSDDALQACELRTGIVLHPAAGEVGVTENTPANGLVPAGDSEAAQEALKFNTYALMLVCSLEADGFLVMASFDSKTVEMDVVKKALDQLQVVVRQLCEDGCGEKKVGQLECLTEKDHEEVKKLAGVKNLTAESDPFVGGVTAEDVEGVWIVDAADTARLVPVCAAGELIVETSKELDAPVTLLAETPAWYEGNKKLYKTGQLAKFKVDGDSASLCILKASEPKTEVAKKKVASSVTPSAVAATSAKQKTLRKIWAKLLKVKEDSIYLGDSFFNRGGDSITAMKLVSEARQQGLKISVAQVFANRTLFDMANVMQTTGTVVDASEKVETEYKPFSLLEENNKASIMEVVKHSLADKSWTIADILPTRPLQEIAVRGTVELPRFSLRYELMHFNGQVDKAALFRACHEVVARNEILRTVFVRDASNTCYGAVIDNLITSIAEYEIDDHADVKDFAAQVCRLDSQTRMPYGSSFVKWFFITNGTSQCSLAFRLSHAQYDEICLPIMLHQFHQLYLNSPESSIPPSYPFSHFVAHTLRDSIPAAIPYWRNLLSGSSGITRFRPSTPITSRKHFAIHRAVDISSRPRDVTIATLPSAAWALALARHVKSPDVLFGEVSSGRSVDVPGIRDTNAVTGPTWQYVPARIQFDNPSKPLRTGHDLLVEMQTQHMASSAYDSMGLEEIVKTCTDWELQKDMWFDTVVHQDVAHVEKLEFSSVSVSGDGNGEEEKKTGAKAEFETMYCFEEPLREWKVQAFRDEKGEEMVLEIVTMECWKEEAEVVLGEVCEALKQLVERPWEGLDVARLEE

Target ORF information:

RefSeq Version	XM_958318.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A non-ribosomal peptide synthetase (NCU08441), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_958318.3

ORF Insert Sequence:

ATGCAGCAAG TCGTGCAAGG AGCCCTCTCT TTTCTCGGCC TCAACGGCCA GAGCAGCAAC 
GCCACCAGCC ACCTCTTCGA CACACTAGGT CTGGAGACAG TATCCAGGCC ACTATCACTA 
CCATCACAAA AGAACATCTC TTTGCTAGAC GCCTCCCTCC TAGCAGGGCT GTTGCTTCTC 
CAGAGAGAGC GAGGAGAAGA TGATCGGGTA GAAAACTTCA ACTGGGGACG GAGAACATCT 
GATGGGGCGG AAACGTCGTC ACAGTTTTCC GTCAAGGCAG TGCTGGGTTC TGAAGGACTG 
GACCAGACGG CGAGTGTGAA GAATGTACTT GAGGTGCTGA AGAAGGTCAC GGGGCAAGAT 
GTGACAGAGG AGAACAGGAC CCTGTTCTTT AACGATGGGA AAGAGGCGCC GGAGAAGATC 
AAGGAAGAGG GATATGAGAC TGCTCAGCGG CCGAACTGGA AATTCCAGCT CGAGCTCCTT 
GCCTCAGAAG ACGGCGAGCA GCATGACCTC ACCCTCCAAG CCTACTGGTT CAAGAACGTT 
CCCGACGCTC TCACCAGTTC CCAAGTCACC ATTCGCCTGA ACGCCTTTAC CGAGCTTCTT 
GGCCTCATCT TCTCCTCTCC CGAGACAACC CCCATCTCCT CCCTCCTCGC CCCTCTCCCC 
TGTGACCTCA ATCAGATCTG GGCCTTCAAC GCCGTCGTGC CCCCATTGAT CGATCGCACT 
ATGCAGGACA TCATCTCCGA GCAAGCCGCC GCCAACCCCT CCAAGCCGGC CGTCTCCTCC 
TGGGACGGCG AGTTCACCTA CGCCGAGGTG GAATCGAAGT CCGACCTTTT GGCTCGCCAC 
TTGGTCTCCC GCGGCGTCAA GCTCGGCGGC GTGGTCCCGC TCATGTTTGA GAAATCCCGC 
TGGACTATCG TCGCCCTGCT GGCCGTAATG AAAGCCGGCT CAGCATTTGC TCTCACCGAT 
CCCACTTCGC AGCCTGAAGG CCGTTTGCGC GTGATGGTCG AGCAGACCGG TGGCGACATC 
ATCGTCGCTT CTGCTTCCCA GACTGAACTC GCGGAGGTCT GTCTCCCCTC TTCTTCTGGA 
GGAAGCCAAG GACAGGTCAT CACCGTCTCT GACGACCTTT TCACCACCCT CTCTCAAACC 
CCCGAGCTCA CCTCGGTGAC GCTGCCCGTG ATCCCCACGC AAACAACACC CCTCTACATC 
CAATTCACCT CTGGCTCCAC CGGCAAACCA AAAGGCGTCG TCATCTCCCA CGCCAACTAC 
ACCTCCGGCG CCCTGCCTCG CGCCGAAGCA GTCGGTTACA AGTCTTCCTC CCGCTGCTTC 
GAGTTCGCTT CCTACGCTTT TGACGTCTCC ATCGATTGCA TGCTCTGCAC CCTCGCTGTT 
GGTGGAACCA TCTGCATTCC GTCGGACGCT GATCGTATGA ACGATCTCGG GGGCGCTATC 
CTCAAGAGCG GCGCAAACAT GGCGCACATG ACTCCTTCCG TTGCCCGAGT GCTTGATCCA 
GAAGTTATCA AGGGTCTTGA TGTCCTTGGC TTGGGCGGTG AAGCAGTCTC TGCCGCGGAT 
GCAGCTGCTT GGTCGAAGGG CAAGACATCG GTCATTGTTG CGTATGGACC GAGTGAATGC 
ACTGTTGGAT GTACCGTCAA CAACGTCTTT GCGAGAGACG AGGAGGCCAA GAAGAAGGGG 
TTCACCACCG GAAACATCGG TACCGGCGTT GGCGGGTGCG GATGGGTTGT TGATCCCAAC 
GATCATGACC GTCTCGTGCC CGTGGGAGCG GTGGGTGAAT TGCTCATGGA GGGCCCGGTC 
GTGGGACTTG GGTACCTCGG TGAAGAGGAA AAGACCAAGG AGGTGTTCAT TGAAGATCCC 
AAGTGGCTCG TTGCTGGCCA TGGCGATGCT CACCCGGGAA GAAAAGGAAG GTTGTACAAG 
ACTGGTGATT TGGTCCGGTA CGATGCCGAT GGCTCGGGGG CTTTTGTCTT TGTTGGACGC 
AAAGATGCGC AGGTCAAGCT GAGAGGCCAA AGAGTAGAGT TGGTTGAGAT TGAACACCAT 
TTGCGCGCCA AGTTGCCCAG TGGCGTGAAG ATTGCTGCCG AGGTTATCAA GCCTAGTGGT 
GGTGATCCCA CGCTGGTTGC TTTCCTGGCG GAGACTAGCT CGACGGGTAT CTCGGCCAAG 
GACGCCCTGC AGAGTGTTGT TGAGGGTGAG GTTAGCTTCT CTCCTGAACT GCAGGCTGCG 
CTGGATGGGA TTGAGGAGGT GCTGGGCATT GATCTGCCTA GGTACATGGT TCCTAATGCG 
TACATCCCCC TTCGTGAGAT GCCCTCGCTG GTGTCGGGCA AGATTGATCG CAAGACGCTG 
AGGGCTATGG GAGCTGCGAT GACGAGGGAG CAAGTCTCTG GTAGCGCAAA GAAGGCCAGA 
CAGGGTGGCG AGGGCGGTGC TCCTACTACC GAGATGGAGA TTGCTCTTCA CAAGGTCTGG 
AAGCAGCTGC TTGGTACCGA GCAGGAGATT GCCGCTGGCG ATAGCTTCTT TGCTGTTGGC 
GGTGACTCTC TCAGGGCCAT GAGACTTATT GCTGCTGCCA GAGCGGAGGG CGTCAAGCTC 
ACTGTCGCTG ATGTCTTCCG GTATCCCGTA CTCAGGGACA TGGCTGTCGT CGCCACCAAG 
GTGGACACCC AGGACGCGGG TGGCAAGGAC AACAACGAGA TTGCTCCCTT CTCTCTGCTT 
GAAAGCGATT GGGCCATCGA AGACGTCAAG GCCGAGGCCA GCAAGAACTG CAATATCGAC 
AAGGACGCCC TCGAGGACGT CTATCCCTGC ACTCCCCTCC AGGAAGCCCT CATGGCTTTG 
TCCGCCAAGG TCAAGGAAGC TTACGTTGCC CAGCGTGTGC TCAAGATGGA AGGCCAAGCC 
GCCGCTGACA AGCTCAAGGC CGCCTTCGAA GCCATCAACG CCGACTGCGC CATCCTCCGC 
ACCCGCATCA TCCACTCCCC GCGCGGCCTG ATGCAAGTCG TCGTCAAGGA GCCTCTGCAC 
TGGCACTCCG CTACCACCCT CGCCGAGTAC CTTGAAACTG ACCGCAACGA GGAAATGGAC 
CTCGGCAAGC CTCTCGTGCG CTACGGCCTC ATCCAGGACG GCGACGCCTA CCACATTGTC 
CTGACCATGC ACCACGCTTT GTACGACGGC TGGTCCATGC CCCTTGTCGT CGACCGCGTC 
AACCAGGCCT ACCAAGGTCT CGTCCCGCGC AAGCCCGCCG CGGAGTTCAA GCACTTCATC 
AACTACCTCA ACAACACCCT CGACCGCGCC GCCTGCGACG CCTACTGGAA GGACCAACTC 
GCCGGCGCCA CGGGTGTGCA GTTCCCCGCT CTCCCCTTTG AGGGGTACCA AACCCGCGCC 
GACTCCCTCT GGGAGCTCTA CGTCTCTCTC GACGGGCGCA AACCCCCTTC TTCGCCCAAC 
ACCACCATCA CCCTCGCCAC CATCGTGCGC GCTGCCTGGG CCCTGGTAGC CAGCCAGTAC 
ACAGCCGGCA ACAACGACAT TGTCTTTGGC GAGACGCTCA CGGGCCGTAA CGCGCCCATC 
GTCGGCGCAG ATGAGATCGA AGGTCCCATG ATCACCACCG TTCCTGTTCG CGTGTGTATT 
GATCGCGAGC AGACCGTCGC CGAGTACTTG CAGCAGGTGG CAGAGCAAAT GATCACGCAA 
ATCCCTTACG AACACGCAGG TCTGCAGCAC ATCCGTCGGT TGTCCGATGA CGCGCTGCAA 
GCGTGCGAGT TGAGAACGGG TATTGTGCTG CATCCTGCTG CGGGCGAGGT GGGCGTTACG 
GAGAACACGC CGGCGAATGG ACTGGTTCCT GCGGGTGATA GCGAGGCGGC GCAGGAGGCG 
TTGAAGTTTA ACACGTATGC GTTGATGCTG GTTTGCTCGT TGGAGGCGGA TGGGTTCTTG 
GTCATGGCGT CTTTTGATAG TAAGACGGTG GAGATGGATG TGGTGAAGAA GGCGTTGGAT 
CAGCTGCAGG TGGTGGTGAG GCAGTTGTGT GAAGACGGTT GTGGGGAGAA GAAGGTGGGA 
CAGTTGGAGT GTTTGACGGA GAAGGATCAC GAGGAGGTGA AGAAGTTGGC GGGTGTGAAG 
AACCTGACGG CCGAGTCTGA TCCTTTTGTC GGGGGTGTCA CTGCGGAGGA TGTGGAAGGG 
GTGTGGATTG TTGATGCTGC TGATACTGCC AGGCTGGTGC CCGTTTGTGC GGCGGGCGAG 
TTGATTGTTG AGACCAGCAA GGAACTTGAT GCGCCTGTCA CTCTTCTCGC TGAGACTCCG 
GCCTGGTACG AGGGCAACAA GAAGCTGTAC AAGACTGGCC AGCTCGCCAA GTTCAAGGTT 
GACGGCGATT CTGCCTCCCT TTGCATCCTC AAGGCTTCTG AGCCCAAGAC CGAGGTGGCC 
AAGAAGAAGG TTGCTTCCTC TGTTACTCCC TCTGCTGTTG CTGCTACCTC CGCCAAGCAA 
AAGACGCTGC GCAAGATTTG GGCCAAGCTG CTTAAGGTCA AGGAGGATAG CATCTACCTC 
GGCGATAGCT TCTTCAACCG TGGTGGTGAC TCCATCACTG CCATGAAGCT GGTGTCGGAG 
GCACGCCAGC AGGGCCTCAA GATTAGCGTC GCGCAGGTCT TTGCCAACCG CACGCTCTTC 
GACATGGCCA ATGTCATGCA AACCACCGGT ACCGTGGTTG ACGCCTCGGA GAAGGTTGAG 
ACCGAGTACA AGCCTTTCTC TCTCCTCGAG GAGAATAACA AGGCCAGCAT CATGGAGGTC 
GTCAAGCACA GTCTGGCCGA TAAGTCGTGG ACCATCGCCG ACATCCTCCC CACCCGTCCC 
CTCCAGGAGA TCGCCGTCCG CGGCACCGTC GAGCTCCCCC GTTTCTCTCT CCGCTACGAG 
TTGATGCACT TTAACGGCCA GGTCGACAAA GCCGCCCTCT TCCGCGCGTG CCACGAAGTA 
GTCGCACGCA ACGAGATCCT ACGCACCGTT TTCGTCCGCG ACGCTTCCAA CACCTGTTAC 
GGCGCCGTCA TCGACAACCT CATCACCTCC ATCGCCGAGT ACGAAATCGA CGACCACGCC 
GACGTCAAGG ACTTCGCCGC CCAAGTGTGC CGCCTCGACT CGCAAACCCG CATGCCATAC 
GGTTCCTCCT TTGTGAAATG GTTCTTCATC ACCAACGGCA CTTCCCAATG CTCGCTCGCC 
TTCCGCCTTT CGCACGCCCA ATACGACGAG ATCTGCCTCC CCATCATGCT CCACCAGTTC 
CACCAGCTCT ACCTCAACTC CCCCGAATCC TCCATTCCGC CTTCCTACCC CTTCTCCCAC 
TTCGTCGCAC ACACCCTCCG CGACTCCATC CCCGCCGCCA TTCCCTACTG GCGCAACCTC 
CTCTCCGGTT CAAGCGGGAT CACCCGCTTC CGCCCTTCCA CACCCATCAC GTCCCGGAAG 
CACTTCGCCA TTCACCGCGC CGTCGACATC TCCTCCCGGC CACGCGACGT CACCATCGCC 
ACTTTACCTT CCGCCGCCTG GGCTTTGGCT CTCGCGCGCC ACGTCAAAAG TCCCGACGTC 
CTCTTCGGCG AAGTCTCCTC TGGTCGTAGC GTCGACGTCC CAGGCATCAG GGACACCAAC 
GCCGTCACCG GTCCGACATG GCAATACGTC CCTGCTCGAA TCCAATTCGA TAACCCATCT 
AAACCTCTTC GTACAGGCCA CGATTTGTTG GTGGAGATGC AGACGCAGCA TATGGCTTCT 
TCAGCGTATG ATAGTATGGG ACTGGAAGAG ATTGTCAAGA CGTGTACGGA CTGGGAACTG 
CAAAAAGATA TGTGGTTTGA TACCGTGGTG CATCAGGATG TGGCGCATGT GGAGAAGCTG 
GAGTTTTCTT CTGTTTCTGT TTCTGGGGAT GGAAATGGGG AAGAGGAAAA GAAGACTGGC 
GCAAAGGCGG AGTTTGAAAC GATGTATTGC TTTGAGGAGC CGTTGAGGGA GTGGAAGGTG 
CAGGCTTTTA GGGACGAGAA GGGCGAGGAG ATGGTGTTGG AGATTGTGAC GATGGAGTGC 
TGGAAGGAGG AGGCGGAGGT GGTGTTGGGA GAGGTTTGTG AGGCGTTGAA GCAGTTGGTG 
GAGAGGCCTT GGGAGGGGTT GGATGTTGCG AGGCTGGAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU08441 cDNA ORF clone, Neurospora crassa OR74A

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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