NCU06318 cDNA ORF clone, Neurospora crassa OR74A

The following NCU06318 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU06318 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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ONf01248 XM_957487.2
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Neurospora crassa OR74A ATP-dependent RNA helicase DHX8 (NCU06318), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf01248
    Clone ID Related Accession (Same CDS sequence) XM_957487.2
    Accession Version XM_957487.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product ATP-dependent RNA helicase DHX8
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_957487.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGACGACT TGGAGAGCCT CGAGCTTCTG TCGCTCGTGT CCAAGGTCAC GTCCGAGTTG 
    CAGAACCACT TGGGCGTCAG CGAAAAGACG CTCGCCGAGT TCATCATCGC CCAACGAGTA
    GAATGCGGGT CATTCGACGA GTTCAAGCAG AAGTTGGCCA CCATCGGCGC CGACTTCCCG
    CCCAGCTTGG TTGAGAGTAT CGACAGACTT GTCCTGACCT TGCACCCAAA GTTCAAGGGC
    CAAAACCATG GACAGACCCG CGCCGATGAA AACGAGAAGC ATGGTCAATC TGCCGAGGAA
    AAGACAAAGG TGTTCAAGGG TCTGTCCATC CCGGACAAGG AAGTGGATGT CATCGACGAC
    ACCCTGGCCT TACTCGAAAG CCTCGAGCCT AAGAGAGCGG AGAAAGAACG ACCAAGGAAA
    CGAAGCCGCA CCCCCGAACG AGACGACTAT GCAGACGACT ACGGGAAGAG GAAGCGCAAG
    GAGCGATACC GGTCAAGGTC GAGGTCAAGG TCCAGGTCAA TTTCGCCACC AAGAGGCCGA
    CGGAAGGGCC GATATGATGA TGAATACGAT GGTGATTTCC GACGGCCACC ACCGCGCGAG
    GTGGACGACT CCCCTATTTT ATACAAGGTT TACGAAGGCC ACGTCACGGG AATCAAGGAC
    TTTGGTGCCT TTGTCAACCT GCATGGCGTT AAGGGCAAGG TTGACGGCTT AGTACATGTA
    TCCGCTTTCG GTCAGCGTGT CAATCACCCT TCGGATCTCT TATCCAAGGG TCAGGAAGTC
    AAAGTCAAAG TCATTAAGCT CGAGGGAGGT AGAGTAGGTT TGTCGATGAA GGACGTAGAC
    CAAGAGACGG GCATGGACCT CGCACCGCAA TTGCGGATAG GTTCGGGCGC CAATATGGAG
    GCATTGGGCG GGAGAGGAGG ATACGATACA AAGAATGTTG TCACCAACGG CTTTGGTGAC
    AAGGGAGCAT TCCAAGCGAC AGCAAATGCC AGGCAACACA AGAAGAGGAT GACGTCTCCT
    GAAAGATGGG AGATTCGTCA AATGATTGCG GCAGGCATTG CAAAGGCATC CGATTACCCA
    GAACTTGAAG AGGACTACCA GGCAACACTC AAAGGCGAGG GACAGATGGA GCTTGAGGAG
    GATGTTGATA TCGAGGTTCG AGACGAGGAA CCTCCCTTCC TGGCTGGACA GACCAAACAG
    TCACTCGAGC TGTCACCCAT CCGCGTTGTT AAAGCTCCCG ACGGGTCCCT TAACCGCGCC
    GCCATGGCAG GCACCAATCT TGCCAAAGAA CGAAAGGAAC TGCGGCAGCA GGAGACTGAA
    GCCAAAAAAG CAGAGGAGAA AAAGGTTGAC TTGTCCTCCC AATGGCAAGA TCCTATGGCC
    AACCCGGAGA CCAGAAAGTT TGCTAGTGAT TTGCGCAAAA ACGCTCAAGC AGCACCAGCG
    GCTCCCGATG CTGTTCCTGA GTGGAAGAGG GCCGTTGCAC CCAAAGAAGT GTCGCTAGGC
    AGGCGCACCA ACATGAGCAT CAAGGAGCAA CGAGAATCTC TCCCCGTCTT TGCCTTTAGA
    GAACAACTAA TCACGGCTGT GAGGGAGAAC CAGGTCCTTA TTGTTGTCGG CGAGACGGGA
    TCCGGAAAAA CGACACAGCT TACTCAATAC CTGGCTGAAG CCGGCTTTAC TAACAATGGG
    ATGATTGGAT GTACGCAGCC TCGCCGTGTC GCCGCCGTGT CTGTTGCGAA ACGTGTTTCT
    GAAGAAGTCG GCTGCCAGCT TGGCCAAGAA GTTGGTTATA CCATCAGATT CGAGGACGTC
    ACCAGCCCAG CAACCAAGAT CAAGTATATG ACCGACGGTA TGCTTCAGAG AGAGATCCTG
    ATCGACCCCG ACCTTAAGAG ATACTCGGTC ATTATGCTTG ATGAGGCCCA CGAACGTACC
    ATCGCCACCG ACGTGCTCTT TGCCCTTTTG AAAAAGACAA TGAAGAGAAG GGAGGACCTC
    AAGGTTATCG TCACTAGTGC TACCTTGGAT GCCGACAAGT TCAGCGAGTA CTTCAACCAA
    TGCCCCATCT TTACCATCCC TGGTCGTACA TTCCCTGTCG AGATTCTCTA CTCCCGTGAG
    CCAGAGTCAG ACTACTTGGA TGCCGCTCTC ACCACCGTCA TGCAAATCCA TCTCAGCGAG
    CCAATGGGCG ACATCCTTTT GTTCTTGACA GGACAGGAAG AAATTGACAC GGCTTGCGAA
    ATTCTATACG AACGGATGAA AGCCTTGGGC CCTAACGTTC CTGAGCTTCT CATCTTGCCC
    GTTTACTCTG CCCTTCCTAG CGAGATGCAG AGTAGGATTT TCGACCCAGC GCCGCCAGGG
    AGTCGAAAGG TTGTTATCGC GACCAACATT GCCGAGACAT CCATCACGAT CGACCATATC
    TACTACGTTG TTGATCCAGG GTTCGTCAAG CAGAACGCAT ACGACCCCAA ACTTGGCATG
    GACTCGTTGG TTGTCACTCC TATTTCGCAG GCTCAAGCCA ACCAGAGAGC CGGTCGAGCT
    GGTAGAACAG GACCAGGAAA GTGCTTCCGC CTGTATACAG AAGCCGCATA CCAGTCTGAA
    ATGTTACCTA CCACAATCCC CGAAATCCAG CGCCAGAATC TGTCTAACAC CATCCTCATG
    CTGAAGGCCA TGGGCATCAA CGACCTTCTC CGGTTCGACT TTATGGACCC ACCTCCCGTC
    AATACTATGC TTACAGCCCT GGAAGAGCTC TACGCCCTCG CTGCGCTGGA CGACGAAGGT
    CTTTTGACAC GCCTTGGAAG AAAGATGGCT GATTTTCCAA TGGAGCCAGC CCTTGCTAAA
    GTTCTTATTG CCTCTGTGGA AAAGGGCTGT TCAGACGAGA TGGTGACTAT TGTCGCCATG
    CTCAACCTCC CCAATGTCTT TTATCGCCCC AAGGAGAAGC AAGCTCAGGC GGACCAGAAG
    AAGGCCAAAT TCCACGATCC TCATGGAGAT CATTTGACCC TCCTCAACGT CTACAACTCG
    TGGAAAAACA ACGGGTATGC GAACCCTTGG TGTTTCGAGA ACTTCATTCA GGCACGTTCT
    ATGCGGCGCG CCAAAGATGT GCGTGACCAG ATTGTCAAGA TCATGGATCG CCACAGGCAT
    CCCGTCATCA GCTGTGGCCG CGATACCAAC AAGGTCCGGC AAGCTCTTTG CGCCGGTTTC
    TTCCGCAACG CGGCTCGCAA GGACCCTCAA GAGGGTTACA AGACGCTGAT CGAAGGCACG
    CCGGTGTATC TGCATCCCAG CTCGGCGCTC TTCGGCAAGC AAGCTGAGTG GGTCGTTTAT
    CACACCTTGG TCTTGACCAC CCGTGAGTAC ATGCACTACA CAACCAGCAT CGAGCCGAAG
    TGGCTGGTCG AGGCCGCACC AACCTTCTTC AAAATGGCGC CGACCGACAA GCTATCGAAG
    CGGAAGAAGG CCGAACGGAT CGAACCGCTG TACAACAAGT ACGCCGGCGC TGATGACTGG
    CGTCTTAGTG CGCAGAGGAG AGGCGGACGT GGTGGTGGTG GCGGTGGTAC TTGGGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_962580.1
    CDS268..3807
    Translation

    Target ORF information:

    RefSeq Version XM_957487.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A ATP-dependent RNA helicase DHX8 (NCU06318), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_957487.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGACGACT TGGAGAGCCT CGAGCTTCTG TCGCTCGTGT CCAAGGTCAC GTCCGAGTTG 
    CAGAACCACT TGGGCGTCAG CGAAAAGACG CTCGCCGAGT TCATCATCGC CCAACGAGTA
    GAATGCGGGT CATTCGACGA GTTCAAGCAG AAGTTGGCCA CCATCGGCGC CGACTTCCCG
    CCCAGCTTGG TTGAGAGTAT CGACAGACTT GTCCTGACCT TGCACCCAAA GTTCAAGGGC
    CAAAACCATG GACAGACCCG CGCCGATGAA AACGAGAAGC ATGGTCAATC TGCCGAGGAA
    AAGACAAAGG TGTTCAAGGG TCTGTCCATC CCGGACAAGG AAGTGGATGT CATCGACGAC
    ACCCTGGCCT TACTCGAAAG CCTCGAGCCT AAGAGAGCGG AGAAAGAACG ACCAAGGAAA
    CGAAGCCGCA CCCCCGAACG AGACGACTAT GCAGACGACT ACGGGAAGAG GAAGCGCAAG
    GAGCGATACC GGTCAAGGTC GAGGTCAAGG TCCAGGTCAA TTTCGCCACC AAGAGGCCGA
    CGGAAGGGCC GATATGATGA TGAATACGAT GGTGATTTCC GACGGCCACC ACCGCGCGAG
    GTGGACGACT CCCCTATTTT ATACAAGGTT TACGAAGGCC ACGTCACGGG AATCAAGGAC
    TTTGGTGCCT TTGTCAACCT GCATGGCGTT AAGGGCAAGG TTGACGGCTT AGTACATGTA
    TCCGCTTTCG GTCAGCGTGT CAATCACCCT TCGGATCTCT TATCCAAGGG TCAGGAAGTC
    AAAGTCAAAG TCATTAAGCT CGAGGGAGGT AGAGTAGGTT TGTCGATGAA GGACGTAGAC
    CAAGAGACGG GCATGGACCT CGCACCGCAA TTGCGGATAG GTTCGGGCGC CAATATGGAG
    GCATTGGGCG GGAGAGGAGG ATACGATACA AAGAATGTTG TCACCAACGG CTTTGGTGAC
    AAGGGAGCAT TCCAAGCGAC AGCAAATGCC AGGCAACACA AGAAGAGGAT GACGTCTCCT
    GAAAGATGGG AGATTCGTCA AATGATTGCG GCAGGCATTG CAAAGGCATC CGATTACCCA
    GAACTTGAAG AGGACTACCA GGCAACACTC AAAGGCGAGG GACAGATGGA GCTTGAGGAG
    GATGTTGATA TCGAGGTTCG AGACGAGGAA CCTCCCTTCC TGGCTGGACA GACCAAACAG
    TCACTCGAGC TGTCACCCAT CCGCGTTGTT AAAGCTCCCG ACGGGTCCCT TAACCGCGCC
    GCCATGGCAG GCACCAATCT TGCCAAAGAA CGAAAGGAAC TGCGGCAGCA GGAGACTGAA
    GCCAAAAAAG CAGAGGAGAA AAAGGTTGAC TTGTCCTCCC AATGGCAAGA TCCTATGGCC
    AACCCGGAGA CCAGAAAGTT TGCTAGTGAT TTGCGCAAAA ACGCTCAAGC AGCACCAGCG
    GCTCCCGATG CTGTTCCTGA GTGGAAGAGG GCCGTTGCAC CCAAAGAAGT GTCGCTAGGC
    AGGCGCACCA ACATGAGCAT CAAGGAGCAA CGAGAATCTC TCCCCGTCTT TGCCTTTAGA
    GAACAACTAA TCACGGCTGT GAGGGAGAAC CAGGTCCTTA TTGTTGTCGG CGAGACGGGA
    TCCGGAAAAA CGACACAGCT TACTCAATAC CTGGCTGAAG CCGGCTTTAC TAACAATGGG
    ATGATTGGAT GTACGCAGCC TCGCCGTGTC GCCGCCGTGT CTGTTGCGAA ACGTGTTTCT
    GAAGAAGTCG GCTGCCAGCT TGGCCAAGAA GTTGGTTATA CCATCAGATT CGAGGACGTC
    ACCAGCCCAG CAACCAAGAT CAAGTATATG ACCGACGGTA TGCTTCAGAG AGAGATCCTG
    ATCGACCCCG ACCTTAAGAG ATACTCGGTC ATTATGCTTG ATGAGGCCCA CGAACGTACC
    ATCGCCACCG ACGTGCTCTT TGCCCTTTTG AAAAAGACAA TGAAGAGAAG GGAGGACCTC
    AAGGTTATCG TCACTAGTGC TACCTTGGAT GCCGACAAGT TCAGCGAGTA CTTCAACCAA
    TGCCCCATCT TTACCATCCC TGGTCGTACA TTCCCTGTCG AGATTCTCTA CTCCCGTGAG
    CCAGAGTCAG ACTACTTGGA TGCCGCTCTC ACCACCGTCA TGCAAATCCA TCTCAGCGAG
    CCAATGGGCG ACATCCTTTT GTTCTTGACA GGACAGGAAG AAATTGACAC GGCTTGCGAA
    ATTCTATACG AACGGATGAA AGCCTTGGGC CCTAACGTTC CTGAGCTTCT CATCTTGCCC
    GTTTACTCTG CCCTTCCTAG CGAGATGCAG AGTAGGATTT TCGACCCAGC GCCGCCAGGG
    AGTCGAAAGG TTGTTATCGC GACCAACATT GCCGAGACAT CCATCACGAT CGACCATATC
    TACTACGTTG TTGATCCAGG GTTCGTCAAG CAGAACGCAT ACGACCCCAA ACTTGGCATG
    GACTCGTTGG TTGTCACTCC TATTTCGCAG GCTCAAGCCA ACCAGAGAGC CGGTCGAGCT
    GGTAGAACAG GACCAGGAAA GTGCTTCCGC CTGTATACAG AAGCCGCATA CCAGTCTGAA
    ATGTTACCTA CCACAATCCC CGAAATCCAG CGCCAGAATC TGTCTAACAC CATCCTCATG
    CTGAAGGCCA TGGGCATCAA CGACCTTCTC CGGTTCGACT TTATGGACCC ACCTCCCGTC
    AATACTATGC TTACAGCCCT GGAAGAGCTC TACGCCCTCG CTGCGCTGGA CGACGAAGGT
    CTTTTGACAC GCCTTGGAAG AAAGATGGCT GATTTTCCAA TGGAGCCAGC CCTTGCTAAA
    GTTCTTATTG CCTCTGTGGA AAAGGGCTGT TCAGACGAGA TGGTGACTAT TGTCGCCATG
    CTCAACCTCC CCAATGTCTT TTATCGCCCC AAGGAGAAGC AAGCTCAGGC GGACCAGAAG
    AAGGCCAAAT TCCACGATCC TCATGGAGAT CATTTGACCC TCCTCAACGT CTACAACTCG
    TGGAAAAACA ACGGGTATGC GAACCCTTGG TGTTTCGAGA ACTTCATTCA GGCACGTTCT
    ATGCGGCGCG CCAAAGATGT GCGTGACCAG ATTGTCAAGA TCATGGATCG CCACAGGCAT
    CCCGTCATCA GCTGTGGCCG CGATACCAAC AAGGTCCGGC AAGCTCTTTG CGCCGGTTTC
    TTCCGCAACG CGGCTCGCAA GGACCCTCAA GAGGGTTACA AGACGCTGAT CGAAGGCACG
    CCGGTGTATC TGCATCCCAG CTCGGCGCTC TTCGGCAAGC AAGCTGAGTG GGTCGTTTAT
    CACACCTTGG TCTTGACCAC CCGTGAGTAC ATGCACTACA CAACCAGCAT CGAGCCGAAG
    TGGCTGGTCG AGGCCGCACC AACCTTCTTC AAAATGGCGC CGACCGACAA GCTATCGAAG
    CGGAAGAAGG CCGAACGGAT CGAACCGCTG TACAACAAGT ACGCCGGCGC TGATGACTGG
    CGTCTTAGTG CGCAGAGGAG AGGCGGACGT GGTGGTGGTG GCGGTGGTAC TTGGGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B