crf7-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	crf7-1
Entrez Gene ID	3876976
Full Name	chromatin remodelling factor 7-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_955736.3	XP_960829.3	chromodomain-helicase-DNA-binding protein 4

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Chd3	NP_649111.1
Magnaporthe oryzae (rice blast fungus)	MGG_00736	XP_003718265.1
Neurospora crassa	NCU10337	XP_960829.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following crf7-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crf7-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf11556	XM_955736.3 Latest version!	Neurospora crassa OR74A chromodomain-helicase-DNA-binding protein 4 (crf7-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crf7-1 ( XM_955736.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_960829.3 Neurospora crassa OR74A chromodomain-helicase-DNA-binding protein 4 (crf7-1), mRNA.

Price & Availability↑

CloneID	ONf11556
Clone ID Related Accession (Same CDS sequence)	XM_955736.3
Accession Version	XM_955736.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	chromodomain-helicase-DNA-binding protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026505). On Feb 23, 2015 this sequence version replaced XM_955736.2.

ATGGCAAACA ACGATAACGC CCACCCAAGC TCCGAAGACG AGCTATTCGT GTCAACGGCA 
ACGGAGCCCA TGGCGTATAC CTTTCAATCT GATCTCTCTG AAGACGAGAT TAAGATTGCT 
ACCGCACAGC CAGCGCCCAA ACCAATATCA CCGGCTGCCT CTGTCGCCGA TCGGTCCAGG 
GCGTTTTCTG ACTCCGATGC TGCCATACCG GACCCTCAGC GCTTGATTCC TTCCCCCGCG 
GTGGCTGAAG ATGACGACCC TGCTTTGTTC CTCGAGCAAG CCGCGGCAGC GCTCGCCGCA 
GTGACGAAAG CAGACACCTC GACCAAACCC AAGAAGCGAG AGTCGGAGTC ACTAGCAAGT 
TGGCGTGGAA GTGTCAATGC TTTGACGAAC GGGGTATCAG ACCACAATAT AGCTGGCACT 
AGGCGTGTTG ACATCGAGGT CGCTTTGCCC TGGCTCCCCC CATCGAAGCG AGCAGCATTT 
ACCTACGTTG AGGTTGAGGA TTCGGATGAG GGGACCAAAC ACTATGATAC CCGTAGGCGA 
AGAAATAAGC CCGCTTACGA TGATTACCGG TTCGATGACG AACTCGAGGG GCTCGGCCTG 
CGCAAGCGCA AGAGACGGTA TGAAGACGAT GAAGAACTCT ACGAGGAAAA CTCCGGTGAT 
GATTATGGTG GTCGTCACTC GCTTCATGAC TATGGCTTTG TCAACGGTGC TAAGCGCAGC 
ACGCGTACCA GCGCCCGTGC TTCTAGTGAA AGAAGCATAG CCTATGAAAG CGGCACAGAA 
TCGCGAGCGT TGAGGCCCCG CTCAGCCAAG AAATATCGAG ATTATGACAG GGACGAACTC 
CAAAATTCGG ACGATGACGA TGGTTTTTTC GTTGTCACGT CCGACATCGT CAAGACCAGA 
GGCAGGAAAA AACGGAAATC TACTGCGAAG CTCAAGACGA CAATGGGAGA CGGCGACTCA 
GATATCGAGT TCGAGTCAAA GCGGCGTTCC TCTCGAGCCA ACAAATCCAA GAAAAGCATG 
AAGGAGATTT TCGACGACGA TGATGACGAC GACATCTTCT ATGTTCAGGA CACAAAACCC 
ACAGCACCCC GGATTGCCAG CGTCCGAGAG GTCTTCAAGC CGGCCCCGGT GGACTTTAAG 
GACGCCCACC GTCCGGTATG TGACAGCTGT GGGTACCCTG AAGGTAATCC TAATAAGGGA 
GCCTTGGTTT ACTGTCAAGG CTGTTCCAAT TCTTACCACA AGATATGTCT TGGCACTCGC 
AATACCCGCG AGCAGCGGGT CACCAAAGTC GACACGGACT CCTTTGTCAT GCAATGTCGG 
TTCTGCGTTG GCTCCTACAC CAAGAAGGAC CATCGTGCTC CAAAGTATGA TACTTGCCAA 
TGCTGTCATC AAGCTAACCC GTCCTGTGCT CCCTTTTCTG AGAAAAAGAC ACCCAAACAA 
GAAGAAAAGC TGCGCGAGGA AAACGATGGA GTAGATCCGA TTATGCCAGT AGACCCGAAA 
CTGGTCAACA ATCCTCTCAA TGTCTTGTTC CGTTGCAGCA AATGCCACAG AGGCTGGCAT 
TATGAACACT TGCCTCACCC TAACGATACC CGTGATCCAG CCATCAGCGA TCCTCTCAAT 
CTGCGCAATC ACCGTCTCCA AGAGTATCAA ACGACTTGGC GATGCAAAGA GTGCATTGAT 
ACGGAGGACC TCAAGATTGA TAAGGTGGTG GCGTGGAGAC CAACTCATAT TAAGCCCAGC 
CAATACGTCA GAGGTCAGAC CATCCTCAAC TTCAATGAGG ATGAAATCGA GTATTTGGTC 
AAGTGGGAGA ACAAGTCCTA TGCGCATTGC GTCTGGATGC CAGGTCCATG GATTTACGGT 
GTTAGCAAGG GAAACATGCG TCTTTCTTTC ATCAAGAGGG CTCTCGGAGA GGGCATGGAA 
GACCAGGCAA GTAGGGATGA GCCTGCTGAC GCACTCCTCA AATGGACTGA AAAAGATGCC 
ATTCACGACT CATGGTTGAC CCCCGATATC ATCCTCGATG TGCAATATGC TGCCAAGTCG 
ATCGAAGACA CAAAGAAATA CAAGGCAATG TCGCTTGCCG ACAGGATGGA GTACGATTTG 
AGCCGGATCT TTCACGTGGT TAAGATCTTC GTCAAATTTG AAGGGTTGGG CTACGATGAC 
GCTGTCTGGG ACACTCCACC TCCTGCGGAC TCTTCCATGT ATGACGCCTA TCTTGAGGCC 
TATCGGGAAT ATCTGAACGG CAAGCACTTC AAGTCAGAGC CCTGGCGTAA TCAGAAGGAA 
CGTATTGAAG CCTTCAGGCA GATGGAGTTC AACGGTGCCA TTGAGGTCAA AAAACAACCG 
CCCGGACTTA CTCGTGGCAA GCTGATGACC TATCAGCTTG AGGGTCTCAA CTGGATGCTC 
TACAATTTCC GCCATGACCG CAGCGTTATT CTTGCCGATG AAATGGGTCT TGGAAAGACT 
GTTCAGGTGG TTGCTTTGCT CTCCAGCATG ATCCAGGATA ACCCCAAGAT CTGGCCATTC 
TTGATTGTCG TTCCCAACGC AACGTGCGCC AACTGGCGTC GTGAAATCAA GAAATGGGCC 
CCTGATCTTC GGGTAGTGGC CTACTACGGC GGGCGGGTTT CCCAAACTTT GGCTCTTCAA 
TATGAACTGT TTCCAAATGG CACTAAGGAC ATGAAGGCGC ACGTTGTCAT CATGTCTTAC 
GACTCCATCA AAGACACTGA CACTCGTAGT CGGTTCAACT CCGTCAAATG GGCTGGTCTT 
ATCGTTGACG AGGCACAAGC ACTGAAGAAT GACGAGAACA GTCTTTACAA AGCACTCTCA 
GTCATGAATA TCCCCTTCAA GCTTTTGCTC ACGGGCACAC CGTTGCAGAA CAATAAGCGA 
GAGCTTTTCA ACCTCTTGCA GTTTATTGAC CCGAAGCTAA AAGCGGAGGA ACTTGACGAG 
GAATTCAAGG ATATCACCAA GGAAAATCTT CCAAAGCTCC ATGAGCTGAT CAGGCCATAC 
TTCCTTCGGC GTACCAAGGC CATGGTACTC ACCTTCCTGC CGCCGATGGC CCAGATCATT 
TTACCAGTGA GCATGACGGT TTTGCAGGAA AAGCTCTGCA AGTCGATCAT GGAGCGCAAT 
CCCCAGCTCA TCCGCGCGAT TTTCTCCGCC AACGGGAAGC TGAAATCACA AGACCGCGGC 
TCGTTGAGCA ACATTCTCAT GCAGCTTCGC AAGTGTCTTT GCCATCCCTT CGTCTACAGT 
CAGTCGATCG AAGATCGGAA CTTGTCACCA GAAGTCACCA AGCGAAACCT TATCGAGGCC 
TCCTCCAAGC TTCTGTTGCT TGAAGTCATG CTGCCTAAAT TGAGGGAGCG TGGACACAGA 
GTTTTGATAT TCAGTCAGTT CCTCGACCAG TTGACAATCC TTGAAGACTT CCTCGCCGGT 
ATGGACCTTC CGTATCAACG ACTTGATGGA AGTCAATCTA GTATGGAAAA GCAGAAGAGG 
ATTGATGCCT TCAATGCACC GGATTCCCAA TTATTCTGCA TGCTGCTGTC AACCCGTGCT 
GGTGGTGTCG GTATCAACCT TGCGACTGCC GACACTGTCA TTATCCTCGA TCCCGATTGG 
AATCCGCATC AGGATATCCA GGCGCTTTCG AGAGCTCATC GTATCGGTCA ACAGAAGAAA 
GTGCTCTGCT TCCAGCTCAT GACCGTCGAC TCTGCCGAGG AGAAGATTCT GCAGATTGGT 
CGTAAGAAGA TGGCTCTTGA TCATCTGCTG ATTGAGACCA TGGACGACAA GGAAGGCGAG 
GCTGCTAATG ATGTGGAGAC GGTGCTTAAA CACGGAGCTG CTGCTTTGTT TGGCGAGGGA 
CGCAAGAAGG ACAGGATCGT ATATGATGAA GCGGCAATCG ATAAGCTTCT TGATCGTTCA 
GCCAAGGAAG AAACGAAGCA AGATGGCGAC AAGTCTGCCG AGTCTGCTTT TGCTTTTGCC 
CGCGTTTGGG CACAAGATGA GGGAGGGCTG AAGGATGACG TGGAGACAGA ACAAAAAGCA 
TTAACCATGA GTGTTTGGGA CCAGATTCTT CAGCAGCGAG AAGAAGAGGC ACGCCTCGAG 
AGGGAGAAGG AACTGCAGGC CTTGGGACGT GGTGGCCGAC GACGTGGAAC GGCGAACTAC 
CGCGGACCCA AGTTCGAGTT TGAAGAAGGT CAGGAAGGAG GTGAGAGTGA TCGTGGTGAC 
CAGGACGGTG ACTTCATGGG CGGCGATGAC AGCGATGGGG AAACAAGCGG TAACGAAAAT 
GGGAACAGCC CCCACGGTGA GTTCTTACCC AACGGAGGCA GCTCACGCAA GTCACAGGCA 
AGGAATGCGC AACAAGACGC CCAAGACAGT TCGGACCCCC GGCACCCCCA GCAAACCCGC 
TCGAGGCAAG GCTCCCAAAA CCAACTCGTC GTCCAAATTG CGCCCGCTCC CGGACCAGCA 
GCCCCAAACC CAAAAGGCCG CCCAACTAAG CCCGTCCGAC CTAGCAGCCA CAACCAAGAA 
GTCCACCGCA TTGCCGACTT TAACACCCCC CATGGTCAAA TCCCCGCATC CGGCGGCCTT 
GCCATGCCCT CCCATGCCAT CGGCGGTGAC CTTGCCGTTC ACCCCAGGCC CGTCCCTTCC 
ACTCACCGAC CAAATGGCAA CCCCAACGGG GCATCCAAAC TAACCTCCAA TACCCCTATT 
CTCGTCCCCA GACCAGGAGC CGGAGTTGTA ACTGGAGCCA GAATTCGGCC ACCATCTCTT 
AATGGTCTTC CCAATCTGAA CCCTAACCGA CAGCCTAACA ACCTCCCCTC AGACGTCACT 
AAACAGCCCA AACCCGGTAT TCTCTGGTCT AACCAAGGCC CACCCGTACC GCCAGGTGTT 
CCGCACACTT CATGCTTTGT CTGCGGCTTC GTACACCCTC CTACGTGGAT GTGTCCCGAG 
ATGAGCCAAG AGGTTCCTCT TCGGCTGGCG CTGGATAATC TTAGGCTCAT ACCACACCAA 
ACTCCGGAAA GAATGGAGAT TAAGCGTAAA TTCTTGCTCG AGATGTTGAG GGTGGTGCAG 
CAGAGGAAGG AAGAAGCTAT GAGGGCGAAA CAGCGGCAGC AGTCGGGTCA GCAGACTCAG 
GAGGGTCAGC AGAGCCAACA GGGCCAGCAG GGCCAGCAGA GGCAAGAAGG ACAAGAACCA 
CCGCAGGTTC CTCCATCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_960829.3
CDS	253..5433
Translation	MANNDNAHPSSEDELFVSTATEPMAYTFQSDLSEDEIKIATAQPAPKPISPAASVADRSRAFSDSDAAIPDPQRLIPSPAVAEDDDPALFLEQAAAALAAVTKADTSTKPKKRESESLASWRGSVNALTNGVSDHNIAGTRRVDIEVALPWLPPSKRAAFTYVEVEDSDEGTKHYDTRRRRNKPAYDDYRFDDELEGLGLRKRKRRYEDDEELYEENSGDDYGGRHSLHDYGFVNGAKRSTRTSARASSERSIAYESGTESRALRPRSAKKYRDYDRDELQNSDDDDGFFVVTSDIVKTRGRKKRKSTAKLKTTMGDGDSDIEFESKRRSSRANKSKKSMKEIFDDDDDDDIFYVQDTKPTAPRIASVREVFKPAPVDFKDAHRPVCDSCGYPEGNPNKGALVYCQGCSNSYHKICLGTRNTREQRVTKVDTDSFVMQCRFCVGSYTKKDHRAPKYDTCQCCHQANPSCAPFSEKKTPKQEEKLREENDGVDPIMPVDPKLVNNPLNVLFRCSKCHRGWHYEHLPHPNDTRDPAISDPLNLRNHRLQEYQTTWRCKECIDTEDLKIDKVVAWRPTHIKPSQYVRGQTILNFNEDEIEYLVKWENKSYAHCVWMPGPWIYGVSKGNMRLSFIKRALGEGMEDQASRDEPADALLKWTEKDAIHDSWLTPDIILDVQYAAKSIEDTKKYKAMSLADRMEYDLSRIFHVVKIFVKFEGLGYDDAVWDTPPPADSSMYDAYLEAYREYLNGKHFKSEPWRNQKERIEAFRQMEFNGAIEVKKQPPGLTRGKLMTYQLEGLNWMLYNFRHDRSVILADEMGLGKTVQVVALLSSMIQDNPKIWPFLIVVPNATCANWRREIKKWAPDLRVVAYYGGRVSQTLALQYELFPNGTKDMKAHVVIMSYDSIKDTDTRSRFNSVKWAGLIVDEAQALKNDENSLYKALSVMNIPFKLLLTGTPLQNNKRELFNLLQFIDPKLKAEELDEEFKDITKENLPKLHELIRPYFLRRTKAMVLTFLPPMAQIILPVSMTVLQEKLCKSIMERNPQLIRAIFSANGKLKSQDRGSLSNILMQLRKCLCHPFVYSQSIEDRNLSPEVTKRNLIEASSKLLLLEVMLPKLRERGHRVLIFSQFLDQLTILEDFLAGMDLPYQRLDGSQSSMEKQKRIDAFNAPDSQLFCMLLSTRAGGVGINLATADTVIILDPDWNPHQDIQALSRAHRIGQQKKVLCFQLMTVDSAEEKILQIGRKKMALDHLLIETMDDKEGEAANDVETVLKHGAAALFGEGRKKDRIVYDEAAIDKLLDRSAKEETKQDGDKSAESAFAFARVWAQDEGGLKDDVETEQKALTMSVWDQILQQREEEARLEREKELQALGRGGRRRGTANYRGPKFEFEEGQEGGESDRGDQDGDFMGGDDSDGETSGNENGNSPHGEFLPNGGSSRKSQARNAQQDAQDSSDPRHPQQTRSRQGSQNQLVVQIAPAPGPAAPNPKGRPTKPVRPSSHNQEVHRIADFNTPHGQIPASGGLAMPSHAIGGDLAVHPRPVPSTHRPNGNPNGASKLTSNTPILVPRPGAGVVTGARIRPPSLNGLPNLNPNRQPNNLPSDVTKQPKPGILWSNQGPPVPPGVPHTSCFVCGFVHPPTWMCPEMSQEVPLRLALDNLRLIPHQTPERMEIKRKFLLEMLRVVQQRKEEAMRAKQRQQSGQQTQEGQQSQQGQQGQQRQEGQEPPQVPPS

Target ORF information:

RefSeq Version	XM_955736.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A chromodomain-helicase-DNA-binding protein 4 (crf7-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_955736.3

ORF Insert Sequence:

ATGGCAAACA ACGATAACGC CCACCCAAGC TCCGAAGACG AGCTATTCGT GTCAACGGCA 
ACGGAGCCCA TGGCGTATAC CTTTCAATCT GATCTCTCTG AAGACGAGAT TAAGATTGCT 
ACCGCACAGC CAGCGCCCAA ACCAATATCA CCGGCTGCCT CTGTCGCCGA TCGGTCCAGG 
GCGTTTTCTG ACTCCGATGC TGCCATACCG GACCCTCAGC GCTTGATTCC TTCCCCCGCG 
GTGGCTGAAG ATGACGACCC TGCTTTGTTC CTCGAGCAAG CCGCGGCAGC GCTCGCCGCA 
GTGACGAAAG CAGACACCTC GACCAAACCC AAGAAGCGAG AGTCGGAGTC ACTAGCAAGT 
TGGCGTGGAA GTGTCAATGC TTTGACGAAC GGGGTATCAG ACCACAATAT AGCTGGCACT 
AGGCGTGTTG ACATCGAGGT CGCTTTGCCC TGGCTCCCCC CATCGAAGCG AGCAGCATTT 
ACCTACGTTG AGGTTGAGGA TTCGGATGAG GGGACCAAAC ACTATGATAC CCGTAGGCGA 
AGAAATAAGC CCGCTTACGA TGATTACCGG TTCGATGACG AACTCGAGGG GCTCGGCCTG 
CGCAAGCGCA AGAGACGGTA TGAAGACGAT GAAGAACTCT ACGAGGAAAA CTCCGGTGAT 
GATTATGGTG GTCGTCACTC GCTTCATGAC TATGGCTTTG TCAACGGTGC TAAGCGCAGC 
ACGCGTACCA GCGCCCGTGC TTCTAGTGAA AGAAGCATAG CCTATGAAAG CGGCACAGAA 
TCGCGAGCGT TGAGGCCCCG CTCAGCCAAG AAATATCGAG ATTATGACAG GGACGAACTC 
CAAAATTCGG ACGATGACGA TGGTTTTTTC GTTGTCACGT CCGACATCGT CAAGACCAGA 
GGCAGGAAAA AACGGAAATC TACTGCGAAG CTCAAGACGA CAATGGGAGA CGGCGACTCA 
GATATCGAGT TCGAGTCAAA GCGGCGTTCC TCTCGAGCCA ACAAATCCAA GAAAAGCATG 
AAGGAGATTT TCGACGACGA TGATGACGAC GACATCTTCT ATGTTCAGGA CACAAAACCC 
ACAGCACCCC GGATTGCCAG CGTCCGAGAG GTCTTCAAGC CGGCCCCGGT GGACTTTAAG 
GACGCCCACC GTCCGGTATG TGACAGCTGT GGGTACCCTG AAGGTAATCC TAATAAGGGA 
GCCTTGGTTT ACTGTCAAGG CTGTTCCAAT TCTTACCACA AGATATGTCT TGGCACTCGC 
AATACCCGCG AGCAGCGGGT CACCAAAGTC GACACGGACT CCTTTGTCAT GCAATGTCGG 
TTCTGCGTTG GCTCCTACAC CAAGAAGGAC CATCGTGCTC CAAAGTATGA TACTTGCCAA 
TGCTGTCATC AAGCTAACCC GTCCTGTGCT CCCTTTTCTG AGAAAAAGAC ACCCAAACAA 
GAAGAAAAGC TGCGCGAGGA AAACGATGGA GTAGATCCGA TTATGCCAGT AGACCCGAAA 
CTGGTCAACA ATCCTCTCAA TGTCTTGTTC CGTTGCAGCA AATGCCACAG AGGCTGGCAT 
TATGAACACT TGCCTCACCC TAACGATACC CGTGATCCAG CCATCAGCGA TCCTCTCAAT 
CTGCGCAATC ACCGTCTCCA AGAGTATCAA ACGACTTGGC GATGCAAAGA GTGCATTGAT 
ACGGAGGACC TCAAGATTGA TAAGGTGGTG GCGTGGAGAC CAACTCATAT TAAGCCCAGC 
CAATACGTCA GAGGTCAGAC CATCCTCAAC TTCAATGAGG ATGAAATCGA GTATTTGGTC 
AAGTGGGAGA ACAAGTCCTA TGCGCATTGC GTCTGGATGC CAGGTCCATG GATTTACGGT 
GTTAGCAAGG GAAACATGCG TCTTTCTTTC ATCAAGAGGG CTCTCGGAGA GGGCATGGAA 
GACCAGGCAA GTAGGGATGA GCCTGCTGAC GCACTCCTCA AATGGACTGA AAAAGATGCC 
ATTCACGACT CATGGTTGAC CCCCGATATC ATCCTCGATG TGCAATATGC TGCCAAGTCG 
ATCGAAGACA CAAAGAAATA CAAGGCAATG TCGCTTGCCG ACAGGATGGA GTACGATTTG 
AGCCGGATCT TTCACGTGGT TAAGATCTTC GTCAAATTTG AAGGGTTGGG CTACGATGAC 
GCTGTCTGGG ACACTCCACC TCCTGCGGAC TCTTCCATGT ATGACGCCTA TCTTGAGGCC 
TATCGGGAAT ATCTGAACGG CAAGCACTTC AAGTCAGAGC CCTGGCGTAA TCAGAAGGAA 
CGTATTGAAG CCTTCAGGCA GATGGAGTTC AACGGTGCCA TTGAGGTCAA AAAACAACCG 
CCCGGACTTA CTCGTGGCAA GCTGATGACC TATCAGCTTG AGGGTCTCAA CTGGATGCTC 
TACAATTTCC GCCATGACCG CAGCGTTATT CTTGCCGATG AAATGGGTCT TGGAAAGACT 
GTTCAGGTGG TTGCTTTGCT CTCCAGCATG ATCCAGGATA ACCCCAAGAT CTGGCCATTC 
TTGATTGTCG TTCCCAACGC AACGTGCGCC AACTGGCGTC GTGAAATCAA GAAATGGGCC 
CCTGATCTTC GGGTAGTGGC CTACTACGGC GGGCGGGTTT CCCAAACTTT GGCTCTTCAA 
TATGAACTGT TTCCAAATGG CACTAAGGAC ATGAAGGCGC ACGTTGTCAT CATGTCTTAC 
GACTCCATCA AAGACACTGA CACTCGTAGT CGGTTCAACT CCGTCAAATG GGCTGGTCTT 
ATCGTTGACG AGGCACAAGC ACTGAAGAAT GACGAGAACA GTCTTTACAA AGCACTCTCA 
GTCATGAATA TCCCCTTCAA GCTTTTGCTC ACGGGCACAC CGTTGCAGAA CAATAAGCGA 
GAGCTTTTCA ACCTCTTGCA GTTTATTGAC CCGAAGCTAA AAGCGGAGGA ACTTGACGAG 
GAATTCAAGG ATATCACCAA GGAAAATCTT CCAAAGCTCC ATGAGCTGAT CAGGCCATAC 
TTCCTTCGGC GTACCAAGGC CATGGTACTC ACCTTCCTGC CGCCGATGGC CCAGATCATT 
TTACCAGTGA GCATGACGGT TTTGCAGGAA AAGCTCTGCA AGTCGATCAT GGAGCGCAAT 
CCCCAGCTCA TCCGCGCGAT TTTCTCCGCC AACGGGAAGC TGAAATCACA AGACCGCGGC 
TCGTTGAGCA ACATTCTCAT GCAGCTTCGC AAGTGTCTTT GCCATCCCTT CGTCTACAGT 
CAGTCGATCG AAGATCGGAA CTTGTCACCA GAAGTCACCA AGCGAAACCT TATCGAGGCC 
TCCTCCAAGC TTCTGTTGCT TGAAGTCATG CTGCCTAAAT TGAGGGAGCG TGGACACAGA 
GTTTTGATAT TCAGTCAGTT CCTCGACCAG TTGACAATCC TTGAAGACTT CCTCGCCGGT 
ATGGACCTTC CGTATCAACG ACTTGATGGA AGTCAATCTA GTATGGAAAA GCAGAAGAGG 
ATTGATGCCT TCAATGCACC GGATTCCCAA TTATTCTGCA TGCTGCTGTC AACCCGTGCT 
GGTGGTGTCG GTATCAACCT TGCGACTGCC GACACTGTCA TTATCCTCGA TCCCGATTGG 
AATCCGCATC AGGATATCCA GGCGCTTTCG AGAGCTCATC GTATCGGTCA ACAGAAGAAA 
GTGCTCTGCT TCCAGCTCAT GACCGTCGAC TCTGCCGAGG AGAAGATTCT GCAGATTGGT 
CGTAAGAAGA TGGCTCTTGA TCATCTGCTG ATTGAGACCA TGGACGACAA GGAAGGCGAG 
GCTGCTAATG ATGTGGAGAC GGTGCTTAAA CACGGAGCTG CTGCTTTGTT TGGCGAGGGA 
CGCAAGAAGG ACAGGATCGT ATATGATGAA GCGGCAATCG ATAAGCTTCT TGATCGTTCA 
GCCAAGGAAG AAACGAAGCA AGATGGCGAC AAGTCTGCCG AGTCTGCTTT TGCTTTTGCC 
CGCGTTTGGG CACAAGATGA GGGAGGGCTG AAGGATGACG TGGAGACAGA ACAAAAAGCA 
TTAACCATGA GTGTTTGGGA CCAGATTCTT CAGCAGCGAG AAGAAGAGGC ACGCCTCGAG 
AGGGAGAAGG AACTGCAGGC CTTGGGACGT GGTGGCCGAC GACGTGGAAC GGCGAACTAC 
CGCGGACCCA AGTTCGAGTT TGAAGAAGGT CAGGAAGGAG GTGAGAGTGA TCGTGGTGAC 
CAGGACGGTG ACTTCATGGG CGGCGATGAC AGCGATGGGG AAACAAGCGG TAACGAAAAT 
GGGAACAGCC CCCACGGTGA GTTCTTACCC AACGGAGGCA GCTCACGCAA GTCACAGGCA 
AGGAATGCGC AACAAGACGC CCAAGACAGT TCGGACCCCC GGCACCCCCA GCAAACCCGC 
TCGAGGCAAG GCTCCCAAAA CCAACTCGTC GTCCAAATTG CGCCCGCTCC CGGACCAGCA 
GCCCCAAACC CAAAAGGCCG CCCAACTAAG CCCGTCCGAC CTAGCAGCCA CAACCAAGAA 
GTCCACCGCA TTGCCGACTT TAACACCCCC CATGGTCAAA TCCCCGCATC CGGCGGCCTT 
GCCATGCCCT CCCATGCCAT CGGCGGTGAC CTTGCCGTTC ACCCCAGGCC CGTCCCTTCC 
ACTCACCGAC CAAATGGCAA CCCCAACGGG GCATCCAAAC TAACCTCCAA TACCCCTATT 
CTCGTCCCCA GACCAGGAGC CGGAGTTGTA ACTGGAGCCA GAATTCGGCC ACCATCTCTT 
AATGGTCTTC CCAATCTGAA CCCTAACCGA CAGCCTAACA ACCTCCCCTC AGACGTCACT 
AAACAGCCCA AACCCGGTAT TCTCTGGTCT AACCAAGGCC CACCCGTACC GCCAGGTGTT 
CCGCACACTT CATGCTTTGT CTGCGGCTTC GTACACCCTC CTACGTGGAT GTGTCCCGAG 
ATGAGCCAAG AGGTTCCTCT TCGGCTGGCG CTGGATAATC TTAGGCTCAT ACCACACCAA 
ACTCCGGAAA GAATGGAGAT TAAGCGTAAA TTCTTGCTCG AGATGTTGAG GGTGGTGCAG 
CAGAGGAAGG AAGAAGCTAT GAGGGCGAAA CAGCGGCAGC AGTCGGGTCA GCAGACTCAG 
GAGGGTCAGC AGAGCCAACA GGGCCAGCAG GGCCAGCAGA GGCAAGAAGG ACAAGAACCA 
CCGCAGGTTC CTCCATCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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crf7-1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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