NCU06884 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU06884
Entrez Gene ID	3875403
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_954138.2	XP_959231.2	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU06884 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU06884 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf10992	XM_954138.2 Latest version!	Neurospora crassa OR74A hypothetical protein (NCU06884), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU06884 ( XM_954138.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_959231.2 Neurospora crassa OR74A hypothetical protein (NCU06884), partial mRNA.

Price & Availability↑

CloneID	ONf10992
Clone ID Related Accession (Same CDS sequence)	XM_954138.2
Accession Version	XM_954138.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507). On Feb 23, 2015 this sequence version replaced XM_954138.1. COMPLETENESS: incomplete on the 5' end.

ATGGTATGTG GAAGTGTCAA CAAGCAAATC TCGCGCTTCG AAGTCACAGC GGTCTATACC 
CATCCGCAGG CCACTGCGGA CATTGTCCTG GTCCACGGCC TCAATGGCCA CCCAGAGAAA 
ACATGGACGG CTGCCAACAG CGTGTTCTGG CCCACCGACC TGCTCCCAGC GTCTCTGGAG 
CTGAAAAACC AGCACGCCAA CGTCTTGGTT TACGGCTACA ATGCCGACGT CTACTCAAGG 
GGCACCAGCA CGCCTAGTAA CAACTTCATT CACCAGCACG CCCAGTCTCT GGTCACCTCG 
CTTACATCGT TCCGCAAGAG CGAAGGGACC GAGAGACTGC CAATAATATG GGTGGTGCAC 
AGTTTGGGAG GTATAGTAAC CAAAAGGGCA CTACTATACT CGAATGATAT CCGGGCCCAT 
CAGCAAGAAG ACCTGCGCTC AGTGTTCGTA TCTACGTACG CCATCATCTT CCTCGGCACC 
CCACACCAAG GCTCAGACTT GGCCACCTGG GGCCGCATGC TCCAAGCCAT GTCGGACGCC 
GTCATTCCCC GGCGCTTCTT CGAGACCGAA TCCGTCCTGC TCAAGTCACT CAAGAAGGAC 
AACGAGACCC TGCAGGGAAT CAACAACCAC TTTCTCGACA TCTACCAGCG TTTCAAGATC 
CACATGGTGC ATGAGAACCA TACCACGGAC TTGAGAGGGA CAAGGGCGCT CGTGGTGGAT 
GCGAACTCGG CTAGTCCGCA GTTGCAGGGC GTGATATACT ATGGCATTGA AGCGACACAC 
TCGGGCATGT GCAAGTTTGA TGGGCAGAAC GCGCCTGGCT ATAGGAATGT GTCGACGGCC 
ATTACGGAGT GGGTGGCGGT GGCTAGGGCA GTCATCGACA TACGCTGGGA AGCAGAGGAG 
GAGGAGCGGA AGAAACGGGC TGCGGATGAG TACTATGAGA GGAGTGTGCA GGCTATGCAG 
TCCTACCAAC GCTCGCATTC GCCGGGTTAT AGCAACCAGC AGTTTCCCTC CATCGACAAT 
TACAGCAATG AGACTCTTCT ACTTCAACAC CACCCTCATG GCAACCAGAG ACGTCCCAAG 
CTCCTGACAC CTCTGCCGTC GTCTCCGTCA CAATCTACGT TGTCCACGCG CGAACCTACG 
TTGTCCACGC GCGAACCTAC GTTGTCCACG CGCGAACCTA CGTTGTCCAC GCGCGAACCT 
ACGTTGGTGT TGGAGCCGGG CGTCATCAGC CCTAGCCAGC CCGTTGGTGG GAATAACAAC 
AAACCCCTCA CTACTACTAC CACCGACATT CAACCTCAAC CACCACTTCC AGCGCCCCTT 
ACCCCCCAAC AACCAAACCC ACCTGCAACA CAAACCCACC GTCACTCCCA AACTCAAACT 
TCCTCCCCCG AACAACCAGA ACCCCTATTC ATCCACCCCG ACTCCTTCCG GCCCAACTCC 
TTCTTCGTCG GCCGCGAAGA CGAACTCCGC GGTCTCCACG AAATGCTCCA AGACCGCCGC 
CGCCGTCTCG AAGGCACTTC CGCTGTGCTA ATTCAATGTC TCCCCGGCGG CGGCAAGACG 
CATCTAGCGC GCGAATACGT CTATCGCTAC CGCGATCACT ACCCCGGCGG CGTGTACTGG 
GTGCGCGCCA AGAGCAGAGG GGAGGTGGAG TCAGGTTATT GGAGGGTAGC CAGGATGGAG 
ATTATGCGTA GTAGTACTAC TACTACCACT ACCTCTAACG ATCACGTAAC AAGTTTGGGA 
TTAGGATTGG AAGCCCTGAG GGAAGATGCA AAGACCATGG TGCAGGTTGT CAAGGCATGG 
TTTAATTCCC ACAGGGACTG GTTGTTGGTG TTGGACGGGA TCCAGTTCGA CATCCCAGGC 
TTGTCAGACT TCATTCCGGA CGCAAGGCAC ACGAGTATCA TCTATACCAG CACGGAGAGG 
GCGGTTACGG GCGATCCGAG GTTTGATAAT CCGCAGGTTA TGGAGTTGGG ATTGTTGACT 
CCCAGACAAG CGGTGGAACT GCTGTTGACC GAACTTGACC GGAAACCTAC CTCTACTGTT 
TCTACCTCCT CCGCCAATCA TGCGGGAAAT GATGGCAACG GCCGGAAACA TGGAGGGTGG 
AAAGATGAAG AGGAAGAGAA ACGAGCGCTG GAGCTGGTTA ATCTGATGGG CAGGTTACCG 
CTCATGATCC ATGTCGCGGC GCAGCATCTG AAAGCGACGA GGGAGCCGTT GGCGAGGTAC 
CTAGCGGGGT GGAGGGCAAG TAAGCCGAAG CAGGTGGGCA ATTTGCATGC GTATAAGAAG 
GTCAGGGAGC AGTTGGATGC GAGGGGGAAC GTGGCGGCGT TGAATTTGGT GGGGATGTTG 
GTTTGGTGGG AGCAGTTGGT GCCGGTTGAG ATGGTGGCTT TTGGCCTCTC AGCCTTATCC 
CGCGATACCC CCGTCAAAAC CTCCGATGCG TCCCATAGGA AGACGACGCT CAACAACACC 
CTCAAAGTCC TCATCAAGTT CGCCCTCATC GAACGCACCA CCGAATCCCA CGACAGCATC 
ACACCCCTAA CCCGCAGCGC TAGCACAGTC AGCTCTCGCA CTTCCGCCAA CCGCAGTCTC 
GACACTCCTG GCACGAATCA ACACCGGAAT TACAGTGACT CCTCTGACTC GCTTGACCTC 
TTGCGGATCC ACTCCGTCAT CCAAGCGTTT TTCATCGAGA CGCTGGCAGA GGAAAAAACT 
GCGGTGGCTT GGATCGAGAG GGCTGCGGCG GTTTGGTGTA GGTCTTTTGA CGAGGCGCAT 
AGGCGTATTG AGGCGTCCAA AAGAGAAGGT GGAGGGGAGC ACGCGGAGTT TGAGGGTCCA 
AGGGTGGGAG TGCCGGATGA TTATCGGAGG TACTGCATCC ATGGCCAGAA GTTGTTGCAG 
AACATCGAGA GGTTTGGTAA TGAGAAGAGG GATCCCCAGA GGAGGTTGGA GAAGTGGAGA 
TGGATGGTAA GACAGAGGGT AGAGGAGGCC GGGAGGGGGG TGGAGGAGCT GAGTCGGCTG 
GAGCGGTTGA TGGGGGTTGA AGAGGTGGAG GTGGTAAGTG TGTTTGATGG CAATAATAGT 
GCGAGTCAGG AGGGCAGTAG TATGTCGGAA ACAGATTCGA ATGATACGAC TGCGCCGAGT 
CAAGAGAGTC AAGAGGAAGG TCACGTCGGT CCGGGAAGTT GGGAGAGGGC GTTTGGGGGC 
GCCGCGGTAG ATGAGGGGTT CACGCCGATG GAGTCGCCTA GTAGCTATCA CTTCTCGACT 
GCTTCGGGTC GTCGGAGGCC GTCGGACTTG ACGATGACGA TAGCAGGTCA GGCAGCTTCG 
GGTCCGTTCT CGTGGAAGGA GAGGGGGATG GAAGCCACGG CGCCGTATCC GACCGAAGGA 
GTGATGCCCC CGACTCCTGG ACTGGACAAC AAAATCAGAG GTTTGCTGGA AGACGAAAGG 
ACTGTGGTCC CGGCCGCTGT TTCTGCTTAT TATGAACCTG TTGGCGAACA GTCTCACGAT 
GAAGTTGACC AAGCAGCCCT CACACCTATC GTCGAAACCC CGAGTGTCTA CACGGCCCTA 
GACGGTTTGG AGATTCCCAC GCCCGCTCTT GAAACAGGAC TCTTTGAGGA GTGGAACGCC 
GTCGTTCCCA ACCACCGCGT CATCAAGAAG CAAGAAGCCC GTCGGTACCA CGACCGCGCC 
GGAGCCTGGA GAGATGTTAG GACTGTCGGC GATCCAAGAG TGGGAGTCAC GCTGGATGTG 
GCTGTTGGAG CGTTGGCTGG GAAGCCCGCG ATGGGTGCAG GTACAAGTGT TGTGCCTGTA 
TGGCCACCGC GTAGAACAAG GCGGGTGACG GCCCAGACAC AGAGTGAAGC GCAGCTGGAG 
CTGAACAAAA TTCGACAGAA GGTCGCGGGC ACGCAGGCTG TTATTGCTCA GGCTCAGGCT 
CAGGCTCAGG CTCAGGCTCA GGCGGTTACT GACGGAGGTT TTGGAGGATT GGGTGATAGC 
TTGACGCCGC CAATGCCGCC TAGGAAGATG AGTGGAGGGG GGCTGTCTGC CGTGAGCGAT 
CTTTCTCCTC CTACACCGTC ATCGAGCGGG TTGCTTTCTC CGAGTGGATG GCTGAAGAGG 
TTGAGGGAGG GCCTTGGCTC GAGTGTGCAG AGTGTACCAA CGGCGGTTGG TTCGACATCG 
CCAGGCATGC CTCAGGGTCA GCAGCTTTCT CGATCAGCAC CGCAGGAGGA TGAGCTTGTG 
ATCGTCCCTG GTCCTATCTT TAGAGGAAGT CGGACGTCTC GCTCAAGTCC GGCTGACCTT 
ACCTCCTCTC CTTTTCCACC TCCTTCTTTT GCTGCTACTG CTGATCTCGA TGAATCGTGG 
ATTCGCCCGT CTCGAGCCAC TCATCGATCA TCTGAGCCGT CAAGTCAGGT TGATTGGGAT 
CAAGAAGCTG AGTATCTCTC GTCTTCCCAG CCGATGGTGA ACACTGGGAA AGTGGCTACT 
GGAAGTCCTT CATCGGGGAT GAGCAGAAAC AGCAATCCCA CAGTTCCCGG CACCAACCTG 
AATGCAAATG GCATGGGTAA CAGGCCTTCA CCCATGCAGC TGCAGATGCC TCTGATGCCC 
ATCAGCATCA ACGCTCCTGG GTCAGGCGCT GGTCGACTAC CTCAGAAGAT CCAACAACAG 
CAGCAGCAAC ACCGACCAGC GTCGTACACA GAAACCGAAC CCTCGCCTAG GACAGAGAGT 
GCGTTTCCAG GTGTTGACAC GAGATACTCG CGATGGGACG AGAATCAGAT GCATGCTCAT 
GGCCATCCTC AAAGTACTTC GGCTTCCTAC CAATTTCCCA CGGCCGGTGG TCATGCCCAT 
CAAAAGCCGG CAACTTCCAC CAGTCTCGGA ATGAAGGCCA CCACAGCATC TTCGACTTCA 
GCCCCCCATC CACAACCAGC GCCCGCCATC CTCTGGCGGA ATCTGGGCAG AGGGTTAACG 
AGAAGGAAGA CTAAGGAGCG GATTCAGAAT GTGCTTGGTG CTAATAGCGG TCGATCGCAA 
TCCGTGAGTC TGTCTGCCTT CCCGACTAGA TCACGACGAG GGAGTGGACA TCAGTCTGTG 
TCAGGGCAAC AGCATCCATT GGCGAGTTCA GCCACTTCCA CCACTACTGC TGGAAGTGCA 
ACGCAAGGCC AGGAACATGC GGGACGTCCA GATATGATGG CATCTATATC GAACATGGGC 
GGACAGTTTC CTGGCACCCC TGCTTCTGCC ATTTCTTTCA TGCCGTCGAC TCCAGCACAA 
CAAACCAGCA TTGGCTCAAT CTCCGCGGTA GCTAGCTCAC CCGTTACTCC AGCTCCTACA 
TCAATCTCCT TGCTACAATA TCAGGAACAG CGAAGTTCAT CCTCCCCTGC TCTTGGAACG 
GGAGTAGGGG CAGGAGCAGG CATCCTCCTC AGCGACGGCA CCATCTTCCA ATTTCCTAGC 
AGCAGCGGTG TCACATCAGC TTTCACTCCA GTGACTGATG TCAGCACTGG GTCCCCTATT 
GTCGGTCGAC CGACGGGACA AGAACATGGG TTTCCGCCGC CTGGGTCGCC GGTAGCAAGT 
GGATTGGCAT GGGAAGTGCA GCAGTCACGT TCAAGACCGA TGGCCACGCC TATTCAGGGA 
GGAGGAATGA CAAACGCTGC GCTTGCAATG ACGCCTAGCA CCTCTAGCAT GGGACCGCCA 
ACCATGCCGC CCACGCCCCC GACCGCTACG GGTTTGGGAA TGGCCAGGAG GAAAAGAATC 
GTTCATCAGC TGACTTCGAC TTCTTCGCCT GGGTTACAGT CTCCAAATCC AAATCCCAAT 
ACTCCTCCTC TGAATTCCTC ATTGCCTACG TTACCCTATG AGACTCCGTT GGGTTCGGTG 
CCTCGAACAC TTACTTCACC TGTGCCCTAC GTTCCTGCTT CTCCTAGGGT CCCCGGTGCG 
AATCCCGATG CTGGCACCTC CAGTCTTATA CCCGGTGCTG GTATTGCGAG TACAGCACGA 
GGAGGGGTTC GAGGAACAAG GACGAGAGCT AGAAGCGGAA GTAGTCCTGG ACCTTGGAGG 
CCTTCGACGA TGGCGTCCAT GCCAATGGGG AGTTTGCATA GGCCTTGGAG TCGTGGACAT 
GGGGCGGGTG GAACTGGTTC TGCGTTGCTG TCGAGGTCGG ACAGTCCCCA GTACGGGAAC 
GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_959231.2
CDS	1..6126
Translation	MVCGSVNKQISRFEVTAVYTHPQATADIVLVHGLNGHPEKTWTAANSVFWPTDLLPASLELKNQHANVLVYGYNADVYSRGTSTPSNNFIHQHAQSLVTSLTSFRKSEGTERLPIIWVVHSLGGIVTKRALLYSNDIRAHQQEDLRSVFVSTYAIIFLGTPHQGSDLATWGRMLQAMSDAVIPRRFFETESVLLKSLKKDNETLQGINNHFLDIYQRFKIHMVHENHTTDLRGTRALVVDANSASPQLQGVIYYGIEATHSGMCKFDGQNAPGYRNVSTAITEWVAVARAVIDIRWEAEEEERKKRAADEYYERSVQAMQSYQRSHSPGYSNQQFPSIDNYSNETLLLQHHPHGNQRRPKLLTPLPSSPSQSTLSTREPTLSTREPTLSTREPTLSTREPTLVLEPGVISPSQPVGGNNNKPLTTTTTDIQPQPPLPAPLTPQQPNPPATQTHRHSQTQTSSPEQPEPLFIHPDSFRPNSFFVGREDELRGLHEMLQDRRRRLEGTSAVLIQCLPGGGKTHLAREYVYRYRDHYPGGVYWVRAKSRGEVESGYWRVARMEIMRSSTTTTTTSNDHVTSLGLGLEALREDAKTMVQVVKAWFNSHRDWLLVLDGIQFDIPGLSDFIPDARHTSIIYTSTERAVTGDPRFDNPQVMELGLLTPRQAVELLLTELDRKPTSTVSTSSANHAGNDGNGRKHGGWKDEEEEKRALELVNLMGRLPLMIHVAAQHLKATREPLARYLAGWRASKPKQVGNLHAYKKVREQLDARGNVAALNLVGMLVWWEQLVPVEMVAFGLSALSRDTPVKTSDASHRKTTLNNTLKVLIKFALIERTTESHDSITPLTRSASTVSSRTSANRSLDTPGTNQHRNYSDSSDSLDLLRIHSVIQAFFIETLAEEKTAVAWIERAAAVWCRSFDEAHRRIEASKREGGGEHAEFEGPRVGVPDDYRRYCIHGQKLLQNIERFGNEKRDPQRRLEKWRWMVRQRVEEAGRGVEELSRLERLMGVEEVEVVSVFDGNNSASQEGSSMSETDSNDTTAPSQESQEEGHVGPGSWERAFGGAAVDEGFTPMESPSSYHFSTASGRRRPSDLTMTIAGQAASGPFSWKERGMEATAPYPTEGVMPPTPGLDNKIRGLLEDERTVVPAAVSAYYEPVGEQSHDEVDQAALTPIVETPSVYTALDGLEIPTPALETGLFEEWNAVVPNHRVIKKQEARRYHDRAGAWRDVRTVGDPRVGVTLDVAVGALAGKPAMGAGTSVVPVWPPRRTRRVTAQTQSEAQLELNKIRQKVAGTQAVIAQAQAQAQAQAQAVTDGGFGGLGDSLTPPMPPRKMSGGGLSAVSDLSPPTPSSSGLLSPSGWLKRLREGLGSSVQSVPTAVGSTSPGMPQGQQLSRSAPQEDELVIVPGPIFRGSRTSRSSPADLTSSPFPPPSFAATADLDESWIRPSRATHRSSEPSSQVDWDQEAEYLSSSQPMVNTGKVATGSPSSGMSRNSNPTVPGTNLNANGMGNRPSPMQLQMPLMPISINAPGSGAGRLPQKIQQQQQQHRPASYTETEPSPRTESAFPGVDTRYSRWDENQMHAHGHPQSTSASYQFPTAGGHAHQKPATSTSLGMKATTASSTSAPHPQPAPAILWRNLGRGLTRRKTKERIQNVLGANSGRSQSVSLSAFPTRSRRGSGHQSVSGQQHPLASSATSTTTAGSATQGQEHAGRPDMMASISNMGGQFPGTPASAISFMPSTPAQQTSIGSISAVASSPVTPAPTSISLLQYQEQRSSSSPALGTGVGAGAGILLSDGTIFQFPSSSGVTSAFTPVTDVSTGSPIVGRPTGQEHGFPPPGSPVASGLAWEVQQSRSRPMATPIQGGGMTNAALAMTPSTSSMGPPTMPPTPPTATGLGMARRKRIVHQLTSTSSPGLQSPNPNPNTPPLNSSLPTLPYETPLGSVPRTLTSPVPYVPASPRVPGANPDAGTSSLIPGAGIASTARGGVRGTRTRARSGSSPGPWRPSTMASMPMGSLHRPWSRGHGAGGTGSALLSRSDSPQYGND

Target ORF information:

RefSeq Version	XM_954138.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A hypothetical protein (NCU06884), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_954138.2

ORF Insert Sequence:

ATGGTATGTG GAAGTGTCAA CAAGCAAATC TCGCGCTTCG AAGTCACAGC GGTCTATACC 
CATCCGCAGG CCACTGCGGA CATTGTCCTG GTCCACGGCC TCAATGGCCA CCCAGAGAAA 
ACATGGACGG CTGCCAACAG CGTGTTCTGG CCCACCGACC TGCTCCCAGC GTCTCTGGAG 
CTGAAAAACC AGCACGCCAA CGTCTTGGTT TACGGCTACA ATGCCGACGT CTACTCAAGG 
GGCACCAGCA CGCCTAGTAA CAACTTCATT CACCAGCACG CCCAGTCTCT GGTCACCTCG 
CTTACATCGT TCCGCAAGAG CGAAGGGACC GAGAGACTGC CAATAATATG GGTGGTGCAC 
AGTTTGGGAG GTATAGTAAC CAAAAGGGCA CTACTATACT CGAATGATAT CCGGGCCCAT 
CAGCAAGAAG ACCTGCGCTC AGTGTTCGTA TCTACGTACG CCATCATCTT CCTCGGCACC 
CCACACCAAG GCTCAGACTT GGCCACCTGG GGCCGCATGC TCCAAGCCAT GTCGGACGCC 
GTCATTCCCC GGCGCTTCTT CGAGACCGAA TCCGTCCTGC TCAAGTCACT CAAGAAGGAC 
AACGAGACCC TGCAGGGAAT CAACAACCAC TTTCTCGACA TCTACCAGCG TTTCAAGATC 
CACATGGTGC ATGAGAACCA TACCACGGAC TTGAGAGGGA CAAGGGCGCT CGTGGTGGAT 
GCGAACTCGG CTAGTCCGCA GTTGCAGGGC GTGATATACT ATGGCATTGA AGCGACACAC 
TCGGGCATGT GCAAGTTTGA TGGGCAGAAC GCGCCTGGCT ATAGGAATGT GTCGACGGCC 
ATTACGGAGT GGGTGGCGGT GGCTAGGGCA GTCATCGACA TACGCTGGGA AGCAGAGGAG 
GAGGAGCGGA AGAAACGGGC TGCGGATGAG TACTATGAGA GGAGTGTGCA GGCTATGCAG 
TCCTACCAAC GCTCGCATTC GCCGGGTTAT AGCAACCAGC AGTTTCCCTC CATCGACAAT 
TACAGCAATG AGACTCTTCT ACTTCAACAC CACCCTCATG GCAACCAGAG ACGTCCCAAG 
CTCCTGACAC CTCTGCCGTC GTCTCCGTCA CAATCTACGT TGTCCACGCG CGAACCTACG 
TTGTCCACGC GCGAACCTAC GTTGTCCACG CGCGAACCTA CGTTGTCCAC GCGCGAACCT 
ACGTTGGTGT TGGAGCCGGG CGTCATCAGC CCTAGCCAGC CCGTTGGTGG GAATAACAAC 
AAACCCCTCA CTACTACTAC CACCGACATT CAACCTCAAC CACCACTTCC AGCGCCCCTT 
ACCCCCCAAC AACCAAACCC ACCTGCAACA CAAACCCACC GTCACTCCCA AACTCAAACT 
TCCTCCCCCG AACAACCAGA ACCCCTATTC ATCCACCCCG ACTCCTTCCG GCCCAACTCC 
TTCTTCGTCG GCCGCGAAGA CGAACTCCGC GGTCTCCACG AAATGCTCCA AGACCGCCGC 
CGCCGTCTCG AAGGCACTTC CGCTGTGCTA ATTCAATGTC TCCCCGGCGG CGGCAAGACG 
CATCTAGCGC GCGAATACGT CTATCGCTAC CGCGATCACT ACCCCGGCGG CGTGTACTGG 
GTGCGCGCCA AGAGCAGAGG GGAGGTGGAG TCAGGTTATT GGAGGGTAGC CAGGATGGAG 
ATTATGCGTA GTAGTACTAC TACTACCACT ACCTCTAACG ATCACGTAAC AAGTTTGGGA 
TTAGGATTGG AAGCCCTGAG GGAAGATGCA AAGACCATGG TGCAGGTTGT CAAGGCATGG 
TTTAATTCCC ACAGGGACTG GTTGTTGGTG TTGGACGGGA TCCAGTTCGA CATCCCAGGC 
TTGTCAGACT TCATTCCGGA CGCAAGGCAC ACGAGTATCA TCTATACCAG CACGGAGAGG 
GCGGTTACGG GCGATCCGAG GTTTGATAAT CCGCAGGTTA TGGAGTTGGG ATTGTTGACT 
CCCAGACAAG CGGTGGAACT GCTGTTGACC GAACTTGACC GGAAACCTAC CTCTACTGTT 
TCTACCTCCT CCGCCAATCA TGCGGGAAAT GATGGCAACG GCCGGAAACA TGGAGGGTGG 
AAAGATGAAG AGGAAGAGAA ACGAGCGCTG GAGCTGGTTA ATCTGATGGG CAGGTTACCG 
CTCATGATCC ATGTCGCGGC GCAGCATCTG AAAGCGACGA GGGAGCCGTT GGCGAGGTAC 
CTAGCGGGGT GGAGGGCAAG TAAGCCGAAG CAGGTGGGCA ATTTGCATGC GTATAAGAAG 
GTCAGGGAGC AGTTGGATGC GAGGGGGAAC GTGGCGGCGT TGAATTTGGT GGGGATGTTG 
GTTTGGTGGG AGCAGTTGGT GCCGGTTGAG ATGGTGGCTT TTGGCCTCTC AGCCTTATCC 
CGCGATACCC CCGTCAAAAC CTCCGATGCG TCCCATAGGA AGACGACGCT CAACAACACC 
CTCAAAGTCC TCATCAAGTT CGCCCTCATC GAACGCACCA CCGAATCCCA CGACAGCATC 
ACACCCCTAA CCCGCAGCGC TAGCACAGTC AGCTCTCGCA CTTCCGCCAA CCGCAGTCTC 
GACACTCCTG GCACGAATCA ACACCGGAAT TACAGTGACT CCTCTGACTC GCTTGACCTC 
TTGCGGATCC ACTCCGTCAT CCAAGCGTTT TTCATCGAGA CGCTGGCAGA GGAAAAAACT 
GCGGTGGCTT GGATCGAGAG GGCTGCGGCG GTTTGGTGTA GGTCTTTTGA CGAGGCGCAT 
AGGCGTATTG AGGCGTCCAA AAGAGAAGGT GGAGGGGAGC ACGCGGAGTT TGAGGGTCCA 
AGGGTGGGAG TGCCGGATGA TTATCGGAGG TACTGCATCC ATGGCCAGAA GTTGTTGCAG 
AACATCGAGA GGTTTGGTAA TGAGAAGAGG GATCCCCAGA GGAGGTTGGA GAAGTGGAGA 
TGGATGGTAA GACAGAGGGT AGAGGAGGCC GGGAGGGGGG TGGAGGAGCT GAGTCGGCTG 
GAGCGGTTGA TGGGGGTTGA AGAGGTGGAG GTGGTAAGTG TGTTTGATGG CAATAATAGT 
GCGAGTCAGG AGGGCAGTAG TATGTCGGAA ACAGATTCGA ATGATACGAC TGCGCCGAGT 
CAAGAGAGTC AAGAGGAAGG TCACGTCGGT CCGGGAAGTT GGGAGAGGGC GTTTGGGGGC 
GCCGCGGTAG ATGAGGGGTT CACGCCGATG GAGTCGCCTA GTAGCTATCA CTTCTCGACT 
GCTTCGGGTC GTCGGAGGCC GTCGGACTTG ACGATGACGA TAGCAGGTCA GGCAGCTTCG 
GGTCCGTTCT CGTGGAAGGA GAGGGGGATG GAAGCCACGG CGCCGTATCC GACCGAAGGA 
GTGATGCCCC CGACTCCTGG ACTGGACAAC AAAATCAGAG GTTTGCTGGA AGACGAAAGG 
ACTGTGGTCC CGGCCGCTGT TTCTGCTTAT TATGAACCTG TTGGCGAACA GTCTCACGAT 
GAAGTTGACC AAGCAGCCCT CACACCTATC GTCGAAACCC CGAGTGTCTA CACGGCCCTA 
GACGGTTTGG AGATTCCCAC GCCCGCTCTT GAAACAGGAC TCTTTGAGGA GTGGAACGCC 
GTCGTTCCCA ACCACCGCGT CATCAAGAAG CAAGAAGCCC GTCGGTACCA CGACCGCGCC 
GGAGCCTGGA GAGATGTTAG GACTGTCGGC GATCCAAGAG TGGGAGTCAC GCTGGATGTG 
GCTGTTGGAG CGTTGGCTGG GAAGCCCGCG ATGGGTGCAG GTACAAGTGT TGTGCCTGTA 
TGGCCACCGC GTAGAACAAG GCGGGTGACG GCCCAGACAC AGAGTGAAGC GCAGCTGGAG 
CTGAACAAAA TTCGACAGAA GGTCGCGGGC ACGCAGGCTG TTATTGCTCA GGCTCAGGCT 
CAGGCTCAGG CTCAGGCTCA GGCGGTTACT GACGGAGGTT TTGGAGGATT GGGTGATAGC 
TTGACGCCGC CAATGCCGCC TAGGAAGATG AGTGGAGGGG GGCTGTCTGC CGTGAGCGAT 
CTTTCTCCTC CTACACCGTC ATCGAGCGGG TTGCTTTCTC CGAGTGGATG GCTGAAGAGG 
TTGAGGGAGG GCCTTGGCTC GAGTGTGCAG AGTGTACCAA CGGCGGTTGG TTCGACATCG 
CCAGGCATGC CTCAGGGTCA GCAGCTTTCT CGATCAGCAC CGCAGGAGGA TGAGCTTGTG 
ATCGTCCCTG GTCCTATCTT TAGAGGAAGT CGGACGTCTC GCTCAAGTCC GGCTGACCTT 
ACCTCCTCTC CTTTTCCACC TCCTTCTTTT GCTGCTACTG CTGATCTCGA TGAATCGTGG 
ATTCGCCCGT CTCGAGCCAC TCATCGATCA TCTGAGCCGT CAAGTCAGGT TGATTGGGAT 
CAAGAAGCTG AGTATCTCTC GTCTTCCCAG CCGATGGTGA ACACTGGGAA AGTGGCTACT 
GGAAGTCCTT CATCGGGGAT GAGCAGAAAC AGCAATCCCA CAGTTCCCGG CACCAACCTG 
AATGCAAATG GCATGGGTAA CAGGCCTTCA CCCATGCAGC TGCAGATGCC TCTGATGCCC 
ATCAGCATCA ACGCTCCTGG GTCAGGCGCT GGTCGACTAC CTCAGAAGAT CCAACAACAG 
CAGCAGCAAC ACCGACCAGC GTCGTACACA GAAACCGAAC CCTCGCCTAG GACAGAGAGT 
GCGTTTCCAG GTGTTGACAC GAGATACTCG CGATGGGACG AGAATCAGAT GCATGCTCAT 
GGCCATCCTC AAAGTACTTC GGCTTCCTAC CAATTTCCCA CGGCCGGTGG TCATGCCCAT 
CAAAAGCCGG CAACTTCCAC CAGTCTCGGA ATGAAGGCCA CCACAGCATC TTCGACTTCA 
GCCCCCCATC CACAACCAGC GCCCGCCATC CTCTGGCGGA ATCTGGGCAG AGGGTTAACG 
AGAAGGAAGA CTAAGGAGCG GATTCAGAAT GTGCTTGGTG CTAATAGCGG TCGATCGCAA 
TCCGTGAGTC TGTCTGCCTT CCCGACTAGA TCACGACGAG GGAGTGGACA TCAGTCTGTG 
TCAGGGCAAC AGCATCCATT GGCGAGTTCA GCCACTTCCA CCACTACTGC TGGAAGTGCA 
ACGCAAGGCC AGGAACATGC GGGACGTCCA GATATGATGG CATCTATATC GAACATGGGC 
GGACAGTTTC CTGGCACCCC TGCTTCTGCC ATTTCTTTCA TGCCGTCGAC TCCAGCACAA 
CAAACCAGCA TTGGCTCAAT CTCCGCGGTA GCTAGCTCAC CCGTTACTCC AGCTCCTACA 
TCAATCTCCT TGCTACAATA TCAGGAACAG CGAAGTTCAT CCTCCCCTGC TCTTGGAACG 
GGAGTAGGGG CAGGAGCAGG CATCCTCCTC AGCGACGGCA CCATCTTCCA ATTTCCTAGC 
AGCAGCGGTG TCACATCAGC TTTCACTCCA GTGACTGATG TCAGCACTGG GTCCCCTATT 
GTCGGTCGAC CGACGGGACA AGAACATGGG TTTCCGCCGC CTGGGTCGCC GGTAGCAAGT 
GGATTGGCAT GGGAAGTGCA GCAGTCACGT TCAAGACCGA TGGCCACGCC TATTCAGGGA 
GGAGGAATGA CAAACGCTGC GCTTGCAATG ACGCCTAGCA CCTCTAGCAT GGGACCGCCA 
ACCATGCCGC CCACGCCCCC GACCGCTACG GGTTTGGGAA TGGCCAGGAG GAAAAGAATC 
GTTCATCAGC TGACTTCGAC TTCTTCGCCT GGGTTACAGT CTCCAAATCC AAATCCCAAT 
ACTCCTCCTC TGAATTCCTC ATTGCCTACG TTACCCTATG AGACTCCGTT GGGTTCGGTG 
CCTCGAACAC TTACTTCACC TGTGCCCTAC GTTCCTGCTT CTCCTAGGGT CCCCGGTGCG 
AATCCCGATG CTGGCACCTC CAGTCTTATA CCCGGTGCTG GTATTGCGAG TACAGCACGA 
GGAGGGGTTC GAGGAACAAG GACGAGAGCT AGAAGCGGAA GTAGTCCTGG ACCTTGGAGG 
CCTTCGACGA TGGCGTCCAT GCCAATGGGG AGTTTGCATA GGCCTTGGAG TCGTGGACAT 
GGGGCGGGTG GAACTGGTTC TGCGTTGCTG TCGAGGTCGG ACAGTCCCCA GTACGGGAAC 
GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU06884 cDNA ORF clone, Neurospora crassa OR74A

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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