NCU04472 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU04472
Entrez Gene ID	3873184
Full Name	DEAD/DEAH box helicase
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395800.1	XP_011394102.1	DEAD/DEAH box helicase
XM_011395801.1	XP_011394103.1	DEAD/DEAH box helicase, variant

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	SPAC694.02	NP_594482.1
Magnaporthe oryzae (rice blast fungus)	MGG_07250	XP_003715470.1
Neurospora crassa	NCU04472	XP_957041.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU04472 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU04472 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf10268	XM_011395800.1 Latest version!	Neurospora crassa OR74A DEAD/DEAH box helicase (NCU04472), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ONf10269	XM_011395801.1 Latest version!	Neurospora crassa OR74A DEAD/DEAH box helicase, variant (NCU04472), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU04472 ( XM_011395800.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394102.1 Neurospora crassa OR74A DEAD/DEAH box helicase (NCU04472), mRNA.

Price & Availability↑

CloneID	ONf10268
Clone ID Related Accession (Same CDS sequence)	XM_011395800.1
Accession Version	XM_011395800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	DEAD/DEAH box helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504).

ATGGCTGGTC CCGATAATTC CAGCGAGTCG CCTGGTTACG GCACTGACAC TGGCGACAAC 
CGCCTGAGGA GCTGGTTCCG ATCGTTGTCC CCGACAAAGG TTGACATTGT CGGCGATTTC 
ACCGGCAAAG AGCGCTTTGC CATTCATGGA GAAGCCCTGC TTGCACATTG TCTTCATGCC 
ATGAAAGTCG ACTTTGACTA CGGCTTCCAG ATTCTCCATG CCGTTCATTC AGTTGAGACT 
CTTCTCCAGA GGCTGCATGA TCGAGGATGC AACTTTCACG TCCTCTGGTT CAATCGAGAG 
TATCAACTAT CCATCCCCAC GGACATCCCA GCTGAGATCA CGTACCGATA CCAGCTCACT 
CGAAGCATCC TGATCGAGCA CTTCGCTCAC GCACAGACTG ACAAACAGAC CATTTTCAGT 
CTTGTGTTCA CGAGCCTGGA CAGCAATTCT TTCGCTCAAT ATCTCCAAGA ACATCATCCG 
CACTTTTTCT TGGGATCTAA TGGTACAACG CCGTGGTGGA CGGGTGGCCC GCAACTTCTG 
GTGCCTCTTC ACATGCTGTA CCGCATCAAT TCAGCAGGAT TGCATATCGC GTGCGTTGAG 
AATCTGGAGT TCAAGAGCTC AAGGGCTTTC CTACCGGTAG CCTCTCCCAG AGGGGCGCAA 
AGATCGCTCA ATGGCGACAC TCTTTCTCCA ATTCCCGACA CCTCCTTGAT GGAGGACAAC 
ATTCAACATC GTCTTGGAGG ACTCGCCAAG ACACACAGCC TGACTGCTCG GGAAATAATA 
TCCCTATACT CACTGGCTCA TGTGCTTGCC AACCATGCGC TCGATGCCAG TGAGGGCGAC 
GAGATTGGGC AGCACGTTAA AGCGCTGCTC CTCCAGCTAG TGTTCATGAG ACACTGCAAT 
GTTTCGAGCC GGAGCTTTGA GTCGCGGGGA TCGGGACTCG AGTTGGGCAG CGCCGCCAAT 
TCTTTCATTT GTTCCTTGGC TGAGCCGGCT CAACGCATTC TTGGCTCGTG GAGTTCAGGA 
CCATTCGCAA ACTCAGCATG GGATGCATTT GATCTCTTTG ATGGGCGGCT CTACCTGGCA 
TTACTGCGTT CTTCACACAA CATACACTTG TCCTGCGCTC TTCACGAACA GTTCGTCCAG 
CTCGGTGGAC TACTCGAGCA GCTCTGTGGC TACGATGCCA CTGCAAAGTG GCCGGTCGAT 
CTGACGAATA CCGATGGAGA AGAAGCTCGT TTGAAGATCA GCGATCCCCA ACCTAATCAG 
TTGGTACCGT CGCTGTCTAT ATTGCCTTTC AGCCATCCCG TTCTGGACAA GTATCTTACT 
TCAGTGCACC TACGCACAGA TACTGTCATC TCCTCTCCAG TTGATTACAA AGTGCACAAA 
GAGCTGACGC ATTGGCACAA CAAAAAGCCA ATTGACCCTA AGCAGCCCAC CAAGGCTCCT 
GACTTTTGGG CAAGACGGCG AAACCAACGC TTCATGGCCG ACATCATTGC CTACTCTGCT 
AGTCTTACCG GTGCATCTGG AAAGATTATC GAACCAGAAA TCGTTGTTGT ACGCGGCTCG 
GTCGAAAGAA GCAAGCCCGT TAGTAGACAT GCGTCGAACA AGGGCAAGAA ACAGGCAAAT 
TCCAACATAC ATCTGGAGAT AAGCCGCGCG CAATACGCTC CTAAAGAGGC AGAACGGCAG 
CCCGCTAATA GGCATGCATT GAACAAGGGC AAGAAACAGG CAAATTCCAA CATACATCCG 
GAGATAGGCG GCGCGCAATA CGCTCATCAA GAGGTAGAAC GGCGCAAGGA AGCCAAGTTC 
AAGGTCAAAT CGCTGGCAAT CATCACCTCC TGGGAACAAA GGTGTCTCGA GTTCTCAAAG 
GAGGCATCTC TGGTTAAGCG ATATTTGCTG GTCGAAAAGT ATCTCTTGGG CTTGAGTCCA 
GACCATTTGT CTGTGATTGG GGCGGAAGTC TCCTTGTATC TTTGCCATAT TCTACGCCAA 
ATCCAGGGTC GCTACAACCC GGACACGCTG ACTCACTGCC ATGTTTTGGC CATGCTTTGG 
TCCCGAACTC AGGAGACCGC CAAAATGACC ATGACCAAAG AAGTCCACAC ACTTGTGAAC 
AGAGTTTCAA AGTCTCTCGA ACTCCCCCAT TACAAGATCC CGGCACTCCT TTCAGAGAGA 
AAGCTGCCAT TTGAGTTCCT GGATATTGCG AGCGGGGGTT TACTGCCTCC TGGAAAGTCT 
TCGCTCGACT TCCAGCTCGG ATTTTGTGGA CCGTTCTTTG AACGAAGTTT CGACTCGGCC 
CCAGATTCTC GTGTGCCTTT TGAGCCCGAT GCCTGGCAGA GAAAGGTACT TGACGCTATC 
GATGAGAACA AGAGTCTGCT CGTGATTGCC CCAACGTCGG CTGGCAAGAC GTTCATCTCA 
TTCTACGCTA TGAGGAAGGT TCTCGAAGCC AACAACGACG ATGTACTGGT GTACATTGCC 
CCGACCAAAG CTCTCGTCAA CCAAATTGCC GCCGAGATCC AAGCCCGATT CTCTAAGCAA 
TATGGCCAAG AAGCGCGGTC TGTCTGGGCT ATCCATACTA GGGATTACCG CATCAATAAT 
GTGAAGGGAT GCCAAATTCT TGTAACCGTC CCGGATATCT TGCAGATTCT TCTGCTGGCT 
CCTTCCAATG CAGCAGGCCC AACGTCTTTT TCCCGGAGAA TAAAGAGAAT CATTTTCGAT 
GAGGTTCATT GTATCGGCCA GTCTGAAGAC GGGATTGTTT GGGAGCAGCT TTTGCTGCTT 
GCACCATGTC CCATCATTGC GCTATCGGCC ACCGTGGGAA ACCCCCTCGA ATTCCGAGAC 
TGGCTTTCCA ATGCACAGAA GGCCAAGGGA TTCGATATGG AGATGGTGGT ACATTCATCC 
AGGTACTCAG ACCTCCGCAA GTTCATCCAT GATCCGTCTC CCGGTTCATA TGAATTCGAA 
GGCCTCAAAC CCGTTGAACG TCTCCCATTT CCTGGACTAG ACTCTGATTG CGAGAATCCG 
TTGCCATTCT CGTTTATACA CCCAATCGCA GCTATCATGG ATAGGAGCCG GGACACGCTT 
AACGATGCCA GTCTTGAACC TCGAGACTGT ATCGTGCTTT GGAAGCACAT GACCAAACAT 
CAGATTGGCC AGTATCAAGT TCCAACCTCC CTGGATCCTA AGAAAATGTT GCCGTCGCTC 
GTCAAAAAGT CCGACGTTAC GAAATGGGAG TCGGCTCTCA AGGACGTCTT GGCTGACTGG 
ATGCTTGACC CCAACTCCCC GTTCGACGAA CTTCGAAACG ATTTGCGTGG AGAACGGTTT 
GCTCACCTAT CATCAACGCA CAATCAACCC GAATCCATCG AATCCCCGCC TCGTCAATCT 
GGACCTATTG TATCACGACG TTCCATATTC TCACTTGTGA CTGATCTTAG GTCGCATGGA 
GCATTGCCTG CCATCATCTT TAATTACGAC CGGGTTGGCT GCGAGACTGT TCTTCGAGAA 
CTCTACGAGA CTCTTAAGTC CGCGGAAGCA GCATACAAGC AACACGATTC AAAGTGGCTT 
AAAAAGCTGG CGGGCTTCGA AGAATGGAAG AAACGCCGTG AGAGCGCAAA AACGAAGGAC 
ACAAAGATGA AGCAAACCAA GAAGACCGGC TTGAGAAATG AGGACGACGA TGGCGCAATG 
AGTAAAGGAG ACCGAGCCCG TGACGCAGCA AATCGAGAAC TGAGCGTTTG GGACAGCTTT 
GACCCAGAAG CCCCCCTTCA GCAATTCAGC TTTGCTGATA ACACCAAACT GACACAGGAA 
GAGCTGAATA CGCGATTGAA GTCACTCAGG GCGGATGCCG TCCGTCCTTG GATCCGAGAT 
GCTCTCTTCC GAGGAATCGG CGTACACCAT GCAGGGATGA ACCGCCAATA TAGACAGATA 
GTCGAAATGC TTTTCCGTAA AGGTTATTTG ACTGTGGTCG TCGCGACCGG GACACTTGCC 
ATGGGTCTCA ACATGCCTTG TAAAACAGTT GTCTTTACCG GCGATTCAGT TTTCCTGACT 
GCTCTCAACT ACCGTCAAGC CTCGGGTCGT GCTGGCCGGC GTGGCTTCGA CCTCCTTGGA 
AACGTTGTCT TTCATGGCCT CCATCCGCAC CGGGTTTTTG AAATAATGTC TGCGAGGCTC 
CCGGATCTTC GCGGGCAGTT TCCAACATCG GTCACGCTCA TCCTACGGCT CTTCATCCTT 
CTACACGGAA CGCAGAACTC TGAGTTTGCA GCCAACGCTG TAAAGGGCCT GCTGACCCAA 
AGCAGACTTT GCTTGGGTGG ACCTGATGCG AAAATGTCGA TCGCACATCA TCTTAGATTT 
TCGATTGACT ACCTCAGACG CCAACACCTG TTATCCGAAA ATGGTGTCCC TCTTAACTTC 
TCTGGCCTTG TGGGACATCT GTACTACACA GAGAATGCTG TCTTTGCATT CCATGCCCTC 
CTCAAGGACG GATACTTTCA CGACCTCAGT ACTAGCAACC GAAGCCGGGA AGACGTCATT 
CTTGAGATTA TGCTTGTTCT AAGTCACTTG TTCTGTCGCC ACGATTTGCC CCAACACAAA 
GACAAGCAGT TCCTCGAGCG TGTACATCGC TCTCCGTGTG TTGGAATTCT CCCAGATCTC 
CCCCAGAAAG CAAGTGAGGT CCTGGAGCGC CACAACCAGC AGACCTTGTC CATTTTCCAG 
AACTATGTCA GCTCTTATAC CAATCAACAT CTGACCGGGA TGCCGGACAA CTGTCTCCCC 
TATACCAAGT ACAAGGTTGA TCCTGTCGAT GCTCAACCAA CGCCGGACCT TTCAACTGTA 
CTTCCCTCCT CTGACCGACC TCGGCCGCCC ACGGTTGTTC GATCTCCATT CTCTGCACTC 
TCTGGCTTTA CGGACGAATT TGCCACTATC CACGAGCTTT GCGAGACTGT CCGCGCAGGC 
GTCTTCCTTG AAGAGTCTGC AGTCCCGTAT ATTCCGATGG CACCAAAGGA AACGAATGGA 
GTACCTTGGA ACGCCTACCT TTATGACTTC TTCAAGCATG GAGATATGGA AGCACTCAAG 
ACTGTCAACA TGATCAAGGG TGGCGATATC TGGTACCACC TCAAAGACTT CTCGCTCATT 
CTTTCTTCAA TTGTGACCAG CCTTGCCAAT TTTCTGGAGC TTCCAAATGC CGACGGTGAT 
TTCGGTTTTG AGGAGGAGGA GGATGATGAT GAGCTTGAGA TGGCAGATGA TGATAGACCA 
ATCTTTAGCG GGGAGGAGGG GAATGGGATA AGGAATGATG TCTGCGGCGC TCCCGCTCCA 
CCACCGCAGA AGGAACAGAA GAAGACCAAG GCGAAAAAGG AAGTGGTTGC CGAGTCATGG 
GATGACGAGA TCAGCGATGA AGACACAGAG GAGGACACAA AAACGGCCAA TCACGGTGGC 
AATTCGTGGT CTGGATCAGC CTCTGCTTAC CCTCAGGCGC CCAGATGGGC TGACGACGGC 
GGTCAGTCGT TGATGGAGGT CTTCCGAAAT TTCTCGGTAC TTCGACAGGA GTTTGAGGAA 
AAATTCCGCA AGACATTTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394102.1
CDS	808..6351
Translation	MAGPDNSSESPGYGTDTGDNRLRSWFRSLSPTKVDIVGDFTGKERFAIHGEALLAHCLHAMKVDFDYGFQILHAVHSVETLLQRLHDRGCNFHVLWFNREYQLSIPTDIPAEITYRYQLTRSILIEHFAHAQTDKQTIFSLVFTSLDSNSFAQYLQEHHPHFFLGSNGTTPWWTGGPQLLVPLHMLYRINSAGLHIACVENLEFKSSRAFLPVASPRGAQRSLNGDTLSPIPDTSLMEDNIQHRLGGLAKTHSLTAREIISLYSLAHVLANHALDASEGDEIGQHVKALLLQLVFMRHCNVSSRSFESRGSGLELGSAANSFICSLAEPAQRILGSWSSGPFANSAWDAFDLFDGRLYLALLRSSHNIHLSCALHEQFVQLGGLLEQLCGYDATAKWPVDLTNTDGEEARLKISDPQPNQLVPSLSILPFSHPVLDKYLTSVHLRTDTVISSPVDYKVHKELTHWHNKKPIDPKQPTKAPDFWARRRNQRFMADIIAYSASLTGASGKIIEPEIVVVRGSVERSKPVSRHASNKGKKQANSNIHLEISRAQYAPKEAERQPANRHALNKGKKQANSNIHPEIGGAQYAHQEVERRKEAKFKVKSLAIITSWEQRCLEFSKEASLVKRYLLVEKYLLGLSPDHLSVIGAEVSLYLCHILRQIQGRYNPDTLTHCHVLAMLWSRTQETAKMTMTKEVHTLVNRVSKSLELPHYKIPALLSERKLPFEFLDIASGGLLPPGKSSLDFQLGFCGPFFERSFDSAPDSRVPFEPDAWQRKVLDAIDENKSLLVIAPTSAGKTFISFYAMRKVLEANNDDVLVYIAPTKALVNQIAAEIQARFSKQYGQEARSVWAIHTRDYRINNVKGCQILVTVPDILQILLLAPSNAAGPTSFSRRIKRIIFDEVHCIGQSEDGIVWEQLLLLAPCPIIALSATVGNPLEFRDWLSNAQKAKGFDMEMVVHSSRYSDLRKFIHDPSPGSYEFEGLKPVERLPFPGLDSDCENPLPFSFIHPIAAIMDRSRDTLNDASLEPRDCIVLWKHMTKHQIGQYQVPTSLDPKKMLPSLVKKSDVTKWESALKDVLADWMLDPNSPFDELRNDLRGERFAHLSSTHNQPESIESPPRQSGPIVSRRSIFSLVTDLRSHGALPAIIFNYDRVGCETVLRELYETLKSAEAAYKQHDSKWLKKLAGFEEWKKRRESAKTKDTKMKQTKKTGLRNEDDDGAMSKGDRARDAANRELSVWDSFDPEAPLQQFSFADNTKLTQEELNTRLKSLRADAVRPWIRDALFRGIGVHHAGMNRQYRQIVEMLFRKGYLTVVVATGTLAMGLNMPCKTVVFTGDSVFLTALNYRQASGRAGRRGFDLLGNVVFHGLHPHRVFEIMSARLPDLRGQFPTSVTLILRLFILLHGTQNSEFAANAVKGLLTQSRLCLGGPDAKMSIAHHLRFSIDYLRRQHLLSENGVPLNFSGLVGHLYYTENAVFAFHALLKDGYFHDLSTSNRSREDVILEIMLVLSHLFCRHDLPQHKDKQFLERVHRSPCVGILPDLPQKASEVLERHNQQTLSIFQNYVSSYTNQHLTGMPDNCLPYTKYKVDPVDAQPTPDLSTVLPSSDRPRPPTVVRSPFSALSGFTDEFATIHELCETVRAGVFLEESAVPYIPMAPKETNGVPWNAYLYDFFKHGDMEALKTVNMIKGGDIWYHLKDFSLILSSIVTSLANFLELPNADGDFGFEEEEDDDELEMADDDRPIFSGEEGNGIRNDVCGAPAPPPQKEQKKTKAKKEVVAESWDDEISDEDTEEDTKTANHGGNSWSGSASAYPQAPRWADDGGQSLMEVFRNFSVLRQEFEEKFRKTFA

Target ORF information:

RefSeq Version	XM_011395800.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A DEAD/DEAH box helicase (NCU04472), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395800.1

ORF Insert Sequence:

ATGGCTGGTC CCGATAATTC CAGCGAGTCG CCTGGTTACG GCACTGACAC TGGCGACAAC 
CGCCTGAGGA GCTGGTTCCG ATCGTTGTCC CCGACAAAGG TTGACATTGT CGGCGATTTC 
ACCGGCAAAG AGCGCTTTGC CATTCATGGA GAAGCCCTGC TTGCACATTG TCTTCATGCC 
ATGAAAGTCG ACTTTGACTA CGGCTTCCAG ATTCTCCATG CCGTTCATTC AGTTGAGACT 
CTTCTCCAGA GGCTGCATGA TCGAGGATGC AACTTTCACG TCCTCTGGTT CAATCGAGAG 
TATCAACTAT CCATCCCCAC GGACATCCCA GCTGAGATCA CGTACCGATA CCAGCTCACT 
CGAAGCATCC TGATCGAGCA CTTCGCTCAC GCACAGACTG ACAAACAGAC CATTTTCAGT 
CTTGTGTTCA CGAGCCTGGA CAGCAATTCT TTCGCTCAAT ATCTCCAAGA ACATCATCCG 
CACTTTTTCT TGGGATCTAA TGGTACAACG CCGTGGTGGA CGGGTGGCCC GCAACTTCTG 
GTGCCTCTTC ACATGCTGTA CCGCATCAAT TCAGCAGGAT TGCATATCGC GTGCGTTGAG 
AATCTGGAGT TCAAGAGCTC AAGGGCTTTC CTACCGGTAG CCTCTCCCAG AGGGGCGCAA 
AGATCGCTCA ATGGCGACAC TCTTTCTCCA ATTCCCGACA CCTCCTTGAT GGAGGACAAC 
ATTCAACATC GTCTTGGAGG ACTCGCCAAG ACACACAGCC TGACTGCTCG GGAAATAATA 
TCCCTATACT CACTGGCTCA TGTGCTTGCC AACCATGCGC TCGATGCCAG TGAGGGCGAC 
GAGATTGGGC AGCACGTTAA AGCGCTGCTC CTCCAGCTAG TGTTCATGAG ACACTGCAAT 
GTTTCGAGCC GGAGCTTTGA GTCGCGGGGA TCGGGACTCG AGTTGGGCAG CGCCGCCAAT 
TCTTTCATTT GTTCCTTGGC TGAGCCGGCT CAACGCATTC TTGGCTCGTG GAGTTCAGGA 
CCATTCGCAA ACTCAGCATG GGATGCATTT GATCTCTTTG ATGGGCGGCT CTACCTGGCA 
TTACTGCGTT CTTCACACAA CATACACTTG TCCTGCGCTC TTCACGAACA GTTCGTCCAG 
CTCGGTGGAC TACTCGAGCA GCTCTGTGGC TACGATGCCA CTGCAAAGTG GCCGGTCGAT 
CTGACGAATA CCGATGGAGA AGAAGCTCGT TTGAAGATCA GCGATCCCCA ACCTAATCAG 
TTGGTACCGT CGCTGTCTAT ATTGCCTTTC AGCCATCCCG TTCTGGACAA GTATCTTACT 
TCAGTGCACC TACGCACAGA TACTGTCATC TCCTCTCCAG TTGATTACAA AGTGCACAAA 
GAGCTGACGC ATTGGCACAA CAAAAAGCCA ATTGACCCTA AGCAGCCCAC CAAGGCTCCT 
GACTTTTGGG CAAGACGGCG AAACCAACGC TTCATGGCCG ACATCATTGC CTACTCTGCT 
AGTCTTACCG GTGCATCTGG AAAGATTATC GAACCAGAAA TCGTTGTTGT ACGCGGCTCG 
GTCGAAAGAA GCAAGCCCGT TAGTAGACAT GCGTCGAACA AGGGCAAGAA ACAGGCAAAT 
TCCAACATAC ATCTGGAGAT AAGCCGCGCG CAATACGCTC CTAAAGAGGC AGAACGGCAG 
CCCGCTAATA GGCATGCATT GAACAAGGGC AAGAAACAGG CAAATTCCAA CATACATCCG 
GAGATAGGCG GCGCGCAATA CGCTCATCAA GAGGTAGAAC GGCGCAAGGA AGCCAAGTTC 
AAGGTCAAAT CGCTGGCAAT CATCACCTCC TGGGAACAAA GGTGTCTCGA GTTCTCAAAG 
GAGGCATCTC TGGTTAAGCG ATATTTGCTG GTCGAAAAGT ATCTCTTGGG CTTGAGTCCA 
GACCATTTGT CTGTGATTGG GGCGGAAGTC TCCTTGTATC TTTGCCATAT TCTACGCCAA 
ATCCAGGGTC GCTACAACCC GGACACGCTG ACTCACTGCC ATGTTTTGGC CATGCTTTGG 
TCCCGAACTC AGGAGACCGC CAAAATGACC ATGACCAAAG AAGTCCACAC ACTTGTGAAC 
AGAGTTTCAA AGTCTCTCGA ACTCCCCCAT TACAAGATCC CGGCACTCCT TTCAGAGAGA 
AAGCTGCCAT TTGAGTTCCT GGATATTGCG AGCGGGGGTT TACTGCCTCC TGGAAAGTCT 
TCGCTCGACT TCCAGCTCGG ATTTTGTGGA CCGTTCTTTG AACGAAGTTT CGACTCGGCC 
CCAGATTCTC GTGTGCCTTT TGAGCCCGAT GCCTGGCAGA GAAAGGTACT TGACGCTATC 
GATGAGAACA AGAGTCTGCT CGTGATTGCC CCAACGTCGG CTGGCAAGAC GTTCATCTCA 
TTCTACGCTA TGAGGAAGGT TCTCGAAGCC AACAACGACG ATGTACTGGT GTACATTGCC 
CCGACCAAAG CTCTCGTCAA CCAAATTGCC GCCGAGATCC AAGCCCGATT CTCTAAGCAA 
TATGGCCAAG AAGCGCGGTC TGTCTGGGCT ATCCATACTA GGGATTACCG CATCAATAAT 
GTGAAGGGAT GCCAAATTCT TGTAACCGTC CCGGATATCT TGCAGATTCT TCTGCTGGCT 
CCTTCCAATG CAGCAGGCCC AACGTCTTTT TCCCGGAGAA TAAAGAGAAT CATTTTCGAT 
GAGGTTCATT GTATCGGCCA GTCTGAAGAC GGGATTGTTT GGGAGCAGCT TTTGCTGCTT 
GCACCATGTC CCATCATTGC GCTATCGGCC ACCGTGGGAA ACCCCCTCGA ATTCCGAGAC 
TGGCTTTCCA ATGCACAGAA GGCCAAGGGA TTCGATATGG AGATGGTGGT ACATTCATCC 
AGGTACTCAG ACCTCCGCAA GTTCATCCAT GATCCGTCTC CCGGTTCATA TGAATTCGAA 
GGCCTCAAAC CCGTTGAACG TCTCCCATTT CCTGGACTAG ACTCTGATTG CGAGAATCCG 
TTGCCATTCT CGTTTATACA CCCAATCGCA GCTATCATGG ATAGGAGCCG GGACACGCTT 
AACGATGCCA GTCTTGAACC TCGAGACTGT ATCGTGCTTT GGAAGCACAT GACCAAACAT 
CAGATTGGCC AGTATCAAGT TCCAACCTCC CTGGATCCTA AGAAAATGTT GCCGTCGCTC 
GTCAAAAAGT CCGACGTTAC GAAATGGGAG TCGGCTCTCA AGGACGTCTT GGCTGACTGG 
ATGCTTGACC CCAACTCCCC GTTCGACGAA CTTCGAAACG ATTTGCGTGG AGAACGGTTT 
GCTCACCTAT CATCAACGCA CAATCAACCC GAATCCATCG AATCCCCGCC TCGTCAATCT 
GGACCTATTG TATCACGACG TTCCATATTC TCACTTGTGA CTGATCTTAG GTCGCATGGA 
GCATTGCCTG CCATCATCTT TAATTACGAC CGGGTTGGCT GCGAGACTGT TCTTCGAGAA 
CTCTACGAGA CTCTTAAGTC CGCGGAAGCA GCATACAAGC AACACGATTC AAAGTGGCTT 
AAAAAGCTGG CGGGCTTCGA AGAATGGAAG AAACGCCGTG AGAGCGCAAA AACGAAGGAC 
ACAAAGATGA AGCAAACCAA GAAGACCGGC TTGAGAAATG AGGACGACGA TGGCGCAATG 
AGTAAAGGAG ACCGAGCCCG TGACGCAGCA AATCGAGAAC TGAGCGTTTG GGACAGCTTT 
GACCCAGAAG CCCCCCTTCA GCAATTCAGC TTTGCTGATA ACACCAAACT GACACAGGAA 
GAGCTGAATA CGCGATTGAA GTCACTCAGG GCGGATGCCG TCCGTCCTTG GATCCGAGAT 
GCTCTCTTCC GAGGAATCGG CGTACACCAT GCAGGGATGA ACCGCCAATA TAGACAGATA 
GTCGAAATGC TTTTCCGTAA AGGTTATTTG ACTGTGGTCG TCGCGACCGG GACACTTGCC 
ATGGGTCTCA ACATGCCTTG TAAAACAGTT GTCTTTACCG GCGATTCAGT TTTCCTGACT 
GCTCTCAACT ACCGTCAAGC CTCGGGTCGT GCTGGCCGGC GTGGCTTCGA CCTCCTTGGA 
AACGTTGTCT TTCATGGCCT CCATCCGCAC CGGGTTTTTG AAATAATGTC TGCGAGGCTC 
CCGGATCTTC GCGGGCAGTT TCCAACATCG GTCACGCTCA TCCTACGGCT CTTCATCCTT 
CTACACGGAA CGCAGAACTC TGAGTTTGCA GCCAACGCTG TAAAGGGCCT GCTGACCCAA 
AGCAGACTTT GCTTGGGTGG ACCTGATGCG AAAATGTCGA TCGCACATCA TCTTAGATTT 
TCGATTGACT ACCTCAGACG CCAACACCTG TTATCCGAAA ATGGTGTCCC TCTTAACTTC 
TCTGGCCTTG TGGGACATCT GTACTACACA GAGAATGCTG TCTTTGCATT CCATGCCCTC 
CTCAAGGACG GATACTTTCA CGACCTCAGT ACTAGCAACC GAAGCCGGGA AGACGTCATT 
CTTGAGATTA TGCTTGTTCT AAGTCACTTG TTCTGTCGCC ACGATTTGCC CCAACACAAA 
GACAAGCAGT TCCTCGAGCG TGTACATCGC TCTCCGTGTG TTGGAATTCT CCCAGATCTC 
CCCCAGAAAG CAAGTGAGGT CCTGGAGCGC CACAACCAGC AGACCTTGTC CATTTTCCAG 
AACTATGTCA GCTCTTATAC CAATCAACAT CTGACCGGGA TGCCGGACAA CTGTCTCCCC 
TATACCAAGT ACAAGGTTGA TCCTGTCGAT GCTCAACCAA CGCCGGACCT TTCAACTGTA 
CTTCCCTCCT CTGACCGACC TCGGCCGCCC ACGGTTGTTC GATCTCCATT CTCTGCACTC 
TCTGGCTTTA CGGACGAATT TGCCACTATC CACGAGCTTT GCGAGACTGT CCGCGCAGGC 
GTCTTCCTTG AAGAGTCTGC AGTCCCGTAT ATTCCGATGG CACCAAAGGA AACGAATGGA 
GTACCTTGGA ACGCCTACCT TTATGACTTC TTCAAGCATG GAGATATGGA AGCACTCAAG 
ACTGTCAACA TGATCAAGGG TGGCGATATC TGGTACCACC TCAAAGACTT CTCGCTCATT 
CTTTCTTCAA TTGTGACCAG CCTTGCCAAT TTTCTGGAGC TTCCAAATGC CGACGGTGAT 
TTCGGTTTTG AGGAGGAGGA GGATGATGAT GAGCTTGAGA TGGCAGATGA TGATAGACCA 
ATCTTTAGCG GGGAGGAGGG GAATGGGATA AGGAATGATG TCTGCGGCGC TCCCGCTCCA 
CCACCGCAGA AGGAACAGAA GAAGACCAAG GCGAAAAAGG AAGTGGTTGC CGAGTCATGG 
GATGACGAGA TCAGCGATGA AGACACAGAG GAGGACACAA AAACGGCCAA TCACGGTGGC 
AATTCGTGGT CTGGATCAGC CTCTGCTTAC CCTCAGGCGC CCAGATGGGC TGACGACGGC 
GGTCAGTCGT TGATGGAGGT CTTCCGAAAT TTCTCGGTAC TTCGACAGGA GTTTGAGGAA 
AAATTCCGCA AGACATTTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU04472 ( XM_011395801.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394103.1 Neurospora crassa OR74A DEAD/DEAH box helicase, variant (NCU04472), mRNA.

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CloneID	ONf10269
Clone ID Related Accession (Same CDS sequence)	XM_011395801.1
Accession Version	XM_011395801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	DEAD/DEAH box helicase, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504).

ATGGCTGGTC CCGATAATTC CAGCGAGTCG CCTGGTTACG GCACTGACAC TGGCGACAAC 
CGCCTGAGGA GCTGGTTCCG ATCGTTGTCC CCGACAAAGG TTGACATTGT CGGCGATTTC 
ACCGGCAAAG AGCGCTTTGC CATTCATGGA GAAGCCCTGC TTGCACATTG TCTTCATGCC 
ATGAAAGTCG ACTTTGACTA CGGCTTCCAG ATTCTCCATG CCGTTCATTC AGTTGAGACT 
CTTCTCCAGA GGCTGCATGA TCGAGGATGC AACTTTCACG TCCTCTGGTT CAATCGAGAG 
TATCAACTAT CCATCCCCAC GGACATCCCA GCTGAGATCA CGTACCGATA CCAGCTCACT 
CGAAGCATCC TGATCGAGCA CTTCGCTCAC GCACAGACTG ACAAACAGAC CATTTTCAGT 
CTTGTGTTCA CGAGCCTGGA CAGCAATTCT TTCGCTCAAT ATCTCCAAGA ACATCATCCG 
CACTTTTTCT TGGGATCTAA TGGTACAACG CCGTGGTGGA CGGGTGGCCC GCAACTTCTG 
GTGCCTCTTC ACATGCTGTA CCGCATCAAT TCAGCAGGAT TGCATATCGC GTGCGTTGAG 
AATCTGGAGT TCAAGAGCTC AAGGGCTTTC CTACCGGTAG CCTCTCCCAG AGGGGCGCAA 
AGATCGCTCA ATGGCGACAC TCTTTCTCCA ATTCCCGACA CCTCCTTGAT GGAGGACAAC 
ATTCAACATC GTCTTGGAGG ACTCGCCAAG ACACACAGCC TGACTGCTCG GGAAATAATA 
TCCCTATACT CACTGGCTCA TGTGCTTGCC AACCATGCGC TCGATGCCAG TGAGGGCGAC 
GAGATTGGGC AGCACGTTAA AGCGCTGCTC CTCCAGCTAG TGTTCATGAG ACACTGCAAT 
GTTTCGAGCC GGAGCTTTGA GTCGCGGGGA TCGGGACTCG AGTTGGGCAG CGCCGCCAAT 
TCTTTCATTT GTTCCTTGGC TGAGCCGGCT CAACGCATTC TTGGCTCGTG GAGTTCAGGA 
CCATTCGCAA ACTCAGCATG GGATGCATTT GATCTCTTTG ATGGGCGGCT CTACCTGGCA 
TTACTGCGTT CTTCACACAA CATACACTTG TCCTGCGCTC TTCACGAACA GTTCGTCCAG 
CTCGGTGGAC TACTCGAGCA GCTCTGTGGC TACGATGCCA CTGCAAAGTG GCCGGTCGAT 
CTGACGAATA CCGATGGAGA AGAAGCTCGT TTGAAGATCA GCGATCCCCA ACCTAATCAG 
TTGGTACCGT CGCTGTCTAT ATTGCCTTTC AGCCATCCCG TTCTGGACAA GTATCTTACT 
TCAGTGCACC TACGCACAGA TACTGTCATC TCCTCTCCAG TTGATTACAA AGTGCACAAA 
GAGCTGACGC ATTGGCACAA CAAAAAGCCA ATTGACCCTA AGCAGCCCAC CAAGGCTCCT 
GACTTTTGGG CAAGACGGCG AAACCAACGC TTCATGGCCG ACATCATTGC CTACTCTGCT 
AGTCTTACCG GTGCATCTGG AAAGATTATC GAACCAGAAA TCGTTGTTGT ACGCGGCTCG 
GTCGAAAGAA GCAAGCCCGT TAGTAGACAT GCGTCGAACA AGGGCAAGAA ACAGGCAAAT 
TCCAACATAC ATCCGGAGAT AGGCGGCGCG CAATACGCTC ATCAAGAGGT AGAACGGCGC 
AAGGAAGCCA AGTTCAAGGT CAAATCGCTG GCAATCATCA CCTCCTGGGA ACAAAGGTGT 
CTCGAGTTCT CAAAGGAGGC ATCTCTGGTT AAGCGATATT TGCTGGTCGA AAAGTATCTC 
TTGGGCTTGA GTCCAGACCA TTTGTCTGTG ATTGGGGCGG AAGTCTCCTT GTATCTTTGC 
CATATTCTAC GCCAAATCCA GGGTCGCTAC AACCCGGACA CGCTGACTCA CTGCCATGTT 
TTGGCCATGC TTTGGTCCCG AACTCAGGAG ACCGCCAAAA TGACCATGAC CAAAGAAGTC 
CACACACTTG TGAACAGAGT TTCAAAGTCT CTCGAACTCC CCCATTACAA GATCCCGGCA 
CTCCTTTCAG AGAGAAAGCT GCCATTTGAG TTCCTGGATA TTGCGAGCGG GGGTTTACTG 
CCTCCTGGAA AGTCTTCGCT CGACTTCCAG CTCGGATTTT GTGGACCGTT CTTTGAACGA 
AGTTTCGACT CGGCCCCAGA TTCTCGTGTG CCTTTTGAGC CCGATGCCTG GCAGAGAAAG 
GTACTTGACG CTATCGATGA GAACAAGAGT CTGCTCGTGA TTGCCCCAAC GTCGGCTGGC 
AAGACGTTCA TCTCATTCTA CGCTATGAGG AAGGTTCTCG AAGCCAACAA CGACGATGTA 
CTGGTGTACA TTGCCCCGAC CAAAGCTCTC GTCAACCAAA TTGCCGCCGA GATCCAAGCC 
CGATTCTCTA AGCAATATGG CCAAGAAGCG CGGTCTGTCT GGGCTATCCA TACTAGGGAT 
TACCGCATCA ATAATGTGAA GGGATGCCAA ATTCTTGTAA CCGTCCCGGA TATCTTGCAG 
ATTCTTCTGC TGGCTCCTTC CAATGCAGCA GGCCCAACGT CTTTTTCCCG GAGAATAAAG 
AGAATCATTT TCGATGAGGT TCATTGTATC GGCCAGTCTG AAGACGGGAT TGTTTGGGAG 
CAGCTTTTGC TGCTTGCACC ATGTCCCATC ATTGCGCTAT CGGCCACCGT GGGAAACCCC 
CTCGAATTCC GAGACTGGCT TTCCAATGCA CAGAAGGCCA AGGGATTCGA TATGGAGATG 
GTGGTACATT CATCCAGGTA CTCAGACCTC CGCAAGTTCA TCCATGATCC GTCTCCCGGT 
TCATATGAAT TCGAAGGCCT CAAACCCGTT GAACGTCTCC CATTTCCTGG ACTAGACTCT 
GATTGCGAGA ATCCGTTGCC ATTCTCGTTT ATACACCCAA TCGCAGCTAT CATGGATAGG 
AGCCGGGACA CGCTTAACGA TGCCAGTCTT GAACCTCGAG ACTGTATCGT GCTTTGGAAG 
CACATGACCA AACATCAGAT TGGCCAGTAT CAAGTTCCAA CCTCCCTGGA TCCTAAGAAA 
ATGTTGCCGT CGCTCGTCAA AAAGTCCGAC GTTACGAAAT GGGAGTCGGC TCTCAAGGAC 
GTCTTGGCTG ACTGGATGCT TGACCCCAAC TCCCCGTTCG ACGAACTTCG AAACGATTTG 
CGTGGAGAAC GGTTTGCTCA CCTATCATCA ACGCACAATC AACCCGAATC CATCGAATCC 
CCGCCTCGTC AATCTGGACC TATTGTATCA CGACGTTCCA TATTCTCACT TGTGACTGAT 
CTTAGGTCGC ATGGAGCATT GCCTGCCATC ATCTTTAATT ACGACCGGGT TGGCTGCGAG 
ACTGTTCTTC GAGAACTCTA CGAGACTCTT AAGTCCGCGG AAGCAGCATA CAAGCAACAC 
GATTCAAAGT GGCTTAAAAA GCTGGCGGGC TTCGAAGAAT GGAAGAAACG CCGTGAGAGC 
GCAAAAACGA AGGACACAAA GATGAAGCAA ACCAAGAAGA CCGGCTTGAG AAATGAGGAC 
GACGATGGCG CAATGAGTAA AGGAGACCGA GCCCGTGACG CAGCAAATCG AGAACTGAGC 
GTTTGGGACA GCTTTGACCC AGAAGCCCCC CTTCAGCAAT TCAGCTTTGC TGATAACACC 
AAACTGACAC AGGAAGAGCT GAATACGCGA TTGAAGTCAC TCAGGGCGGA TGCCGTCCGT 
CCTTGGATCC GAGATGCTCT CTTCCGAGGA ATCGGCGTAC ACCATGCAGG GATGAACCGC 
CAATATAGAC AGATAGTCGA AATGCTTTTC CGTAAAGGTT ATTTGACTGT GGTCGTCGCG 
ACCGGGACAC TTGCCATGGG TCTCAACATG CCTTGTAAAA CAGTTGTCTT TACCGGCGAT 
TCAGTTTTCC TGACTGCTCT CAACTACCGT CAAGCCTCGG GTCGTGCTGG CCGGCGTGGC 
TTCGACCTCC TTGGAAACGT TGTCTTTCAT GGCCTCCATC CGCACCGGGT TTTTGAAATA 
ATGTCTGCGA GGCTCCCGGA TCTTCGCGGG CAGTTTCCAA CATCGGTCAC GCTCATCCTA 
CGGCTCTTCA TCCTTCTACA CGGAACGCAG AACTCTGAGT TTGCAGCCAA CGCTGTAAAG 
GGCCTGCTGA CCCAAAGCAG ACTTTGCTTG GGTGGACCTG ATGCGAAAAT GTCGATCGCA 
CATCATCTTA GATTTTCGAT TGACTACCTC AGACGCCAAC ACCTGTTATC CGAAAATGGT 
GTCCCTCTTA ACTTCTCTGG CCTTGTGGGA CATCTGTACT ACACAGAGAA TGCTGTCTTT 
GCATTCCATG CCCTCCTCAA GGACGGATAC TTTCACGACC TCAGTACTAG CAACCGAAGC 
CGGGAAGACG TCATTCTTGA GATTATGCTT GTTCTAAGTC ACTTGTTCTG TCGCCACGAT 
TTGCCCCAAC ACAAAGACAA GCAGTTCCTC GAGCGTGTAC ATCGCTCTCC GTGTGTTGGA 
ATTCTCCCAG ATCTCCCCCA GAAAGCAAGT GAGGTCCTGG AGCGCCACAA CCAGCAGACC 
TTGTCCATTT TCCAGAACTA TGTCAGCTCT TATACCAATC AACATCTGAC CGGGATGCCG 
GACAACTGTC TCCCCTATAC CAAGTACAAG GTTGATCCTG TCGATGCTCA ACCAACGCCG 
GACCTTTCAA CTGTACTTCC CTCCTCTGAC CGACCTCGGC CGCCCACGGT TGTTCGATCT 
CCATTCTCTG CACTCTCTGG CTTTACGGAC GAATTTGCCA CTATCCACGA GCTTTGCGAG 
ACTGTCCGCG CAGGCGTCTT CCTTGAAGAG TCTGCAGTCC CGTATATTCC GATGGCACCA 
AAGGAAACGA ATGGAGTACC TTGGAACGCC TACCTTTATG ACTTCTTCAA GCATGGAGAT 
ATGGAAGCAC TCAAGACTGT CAACATGATC AAGGGTGGCG ATATCTGGTA CCACCTCAAA 
GACTTCTCGC TCATTCTTTC TTCAATTGTG ACCAGCCTTG CCAATTTTCT GGAGCTTCCA 
AATGCCGACG GTGATTTCGG TTTTGAGGAG GAGGAGGATG ATGATGAGCT TGAGATGGCA 
GATGATGATA GACCAATCTT TAGCGGGGAG GAGGGGAATG GGATAAGGAA TGATGTCTGC 
GGCGCTCCCG CTCCACCACC GCAGAAGGAA CAGAAGAAGA CCAAGGCGAA AAAGGAAGTG 
GTTGCCGAGT CATGGGATGA CGAGATCAGC GATGAAGACA CAGAGGAGGA CACAAAAACG 
GCCAATCACG GTGGCAATTC GTGGTCTGGA TCAGCCTCTG CTTACCCTCA GGCGCCCAGA 
TGGGCTGACG ACGGCGGTCA GTCGTTGATG GAGGTCTTCC GAAATTTCTC GGTACTTCGA 
CAGGAGTTTG AGGAAAAATT CCGCAAGACA TTTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394103.1
CDS	808..6246
Translation	MAGPDNSSESPGYGTDTGDNRLRSWFRSLSPTKVDIVGDFTGKERFAIHGEALLAHCLHAMKVDFDYGFQILHAVHSVETLLQRLHDRGCNFHVLWFNREYQLSIPTDIPAEITYRYQLTRSILIEHFAHAQTDKQTIFSLVFTSLDSNSFAQYLQEHHPHFFLGSNGTTPWWTGGPQLLVPLHMLYRINSAGLHIACVENLEFKSSRAFLPVASPRGAQRSLNGDTLSPIPDTSLMEDNIQHRLGGLAKTHSLTAREIISLYSLAHVLANHALDASEGDEIGQHVKALLLQLVFMRHCNVSSRSFESRGSGLELGSAANSFICSLAEPAQRILGSWSSGPFANSAWDAFDLFDGRLYLALLRSSHNIHLSCALHEQFVQLGGLLEQLCGYDATAKWPVDLTNTDGEEARLKISDPQPNQLVPSLSILPFSHPVLDKYLTSVHLRTDTVISSPVDYKVHKELTHWHNKKPIDPKQPTKAPDFWARRRNQRFMADIIAYSASLTGASGKIIEPEIVVVRGSVERSKPVSRHASNKGKKQANSNIHPEIGGAQYAHQEVERRKEAKFKVKSLAIITSWEQRCLEFSKEASLVKRYLLVEKYLLGLSPDHLSVIGAEVSLYLCHILRQIQGRYNPDTLTHCHVLAMLWSRTQETAKMTMTKEVHTLVNRVSKSLELPHYKIPALLSERKLPFEFLDIASGGLLPPGKSSLDFQLGFCGPFFERSFDSAPDSRVPFEPDAWQRKVLDAIDENKSLLVIAPTSAGKTFISFYAMRKVLEANNDDVLVYIAPTKALVNQIAAEIQARFSKQYGQEARSVWAIHTRDYRINNVKGCQILVTVPDILQILLLAPSNAAGPTSFSRRIKRIIFDEVHCIGQSEDGIVWEQLLLLAPCPIIALSATVGNPLEFRDWLSNAQKAKGFDMEMVVHSSRYSDLRKFIHDPSPGSYEFEGLKPVERLPFPGLDSDCENPLPFSFIHPIAAIMDRSRDTLNDASLEPRDCIVLWKHMTKHQIGQYQVPTSLDPKKMLPSLVKKSDVTKWESALKDVLADWMLDPNSPFDELRNDLRGERFAHLSSTHNQPESIESPPRQSGPIVSRRSIFSLVTDLRSHGALPAIIFNYDRVGCETVLRELYETLKSAEAAYKQHDSKWLKKLAGFEEWKKRRESAKTKDTKMKQTKKTGLRNEDDDGAMSKGDRARDAANRELSVWDSFDPEAPLQQFSFADNTKLTQEELNTRLKSLRADAVRPWIRDALFRGIGVHHAGMNRQYRQIVEMLFRKGYLTVVVATGTLAMGLNMPCKTVVFTGDSVFLTALNYRQASGRAGRRGFDLLGNVVFHGLHPHRVFEIMSARLPDLRGQFPTSVTLILRLFILLHGTQNSEFAANAVKGLLTQSRLCLGGPDAKMSIAHHLRFSIDYLRRQHLLSENGVPLNFSGLVGHLYYTENAVFAFHALLKDGYFHDLSTSNRSREDVILEIMLVLSHLFCRHDLPQHKDKQFLERVHRSPCVGILPDLPQKASEVLERHNQQTLSIFQNYVSSYTNQHLTGMPDNCLPYTKYKVDPVDAQPTPDLSTVLPSSDRPRPPTVVRSPFSALSGFTDEFATIHELCETVRAGVFLEESAVPYIPMAPKETNGVPWNAYLYDFFKHGDMEALKTVNMIKGGDIWYHLKDFSLILSSIVTSLANFLELPNADGDFGFEEEEDDDELEMADDDRPIFSGEEGNGIRNDVCGAPAPPPQKEQKKTKAKKEVVAESWDDEISDEDTEEDTKTANHGGNSWSGSASAYPQAPRWADDGGQSLMEVFRNFSVLRQEFEEKFRKTFA

Target ORF information:

RefSeq Version	XM_011395801.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A DEAD/DEAH box helicase, variant (NCU04472), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395801.1

ORF Insert Sequence:

ATGGCTGGTC CCGATAATTC CAGCGAGTCG CCTGGTTACG GCACTGACAC TGGCGACAAC 
CGCCTGAGGA GCTGGTTCCG ATCGTTGTCC CCGACAAAGG TTGACATTGT CGGCGATTTC 
ACCGGCAAAG AGCGCTTTGC CATTCATGGA GAAGCCCTGC TTGCACATTG TCTTCATGCC 
ATGAAAGTCG ACTTTGACTA CGGCTTCCAG ATTCTCCATG CCGTTCATTC AGTTGAGACT 
CTTCTCCAGA GGCTGCATGA TCGAGGATGC AACTTTCACG TCCTCTGGTT CAATCGAGAG 
TATCAACTAT CCATCCCCAC GGACATCCCA GCTGAGATCA CGTACCGATA CCAGCTCACT 
CGAAGCATCC TGATCGAGCA CTTCGCTCAC GCACAGACTG ACAAACAGAC CATTTTCAGT 
CTTGTGTTCA CGAGCCTGGA CAGCAATTCT TTCGCTCAAT ATCTCCAAGA ACATCATCCG 
CACTTTTTCT TGGGATCTAA TGGTACAACG CCGTGGTGGA CGGGTGGCCC GCAACTTCTG 
GTGCCTCTTC ACATGCTGTA CCGCATCAAT TCAGCAGGAT TGCATATCGC GTGCGTTGAG 
AATCTGGAGT TCAAGAGCTC AAGGGCTTTC CTACCGGTAG CCTCTCCCAG AGGGGCGCAA 
AGATCGCTCA ATGGCGACAC TCTTTCTCCA ATTCCCGACA CCTCCTTGAT GGAGGACAAC 
ATTCAACATC GTCTTGGAGG ACTCGCCAAG ACACACAGCC TGACTGCTCG GGAAATAATA 
TCCCTATACT CACTGGCTCA TGTGCTTGCC AACCATGCGC TCGATGCCAG TGAGGGCGAC 
GAGATTGGGC AGCACGTTAA AGCGCTGCTC CTCCAGCTAG TGTTCATGAG ACACTGCAAT 
GTTTCGAGCC GGAGCTTTGA GTCGCGGGGA TCGGGACTCG AGTTGGGCAG CGCCGCCAAT 
TCTTTCATTT GTTCCTTGGC TGAGCCGGCT CAACGCATTC TTGGCTCGTG GAGTTCAGGA 
CCATTCGCAA ACTCAGCATG GGATGCATTT GATCTCTTTG ATGGGCGGCT CTACCTGGCA 
TTACTGCGTT CTTCACACAA CATACACTTG TCCTGCGCTC TTCACGAACA GTTCGTCCAG 
CTCGGTGGAC TACTCGAGCA GCTCTGTGGC TACGATGCCA CTGCAAAGTG GCCGGTCGAT 
CTGACGAATA CCGATGGAGA AGAAGCTCGT TTGAAGATCA GCGATCCCCA ACCTAATCAG 
TTGGTACCGT CGCTGTCTAT ATTGCCTTTC AGCCATCCCG TTCTGGACAA GTATCTTACT 
TCAGTGCACC TACGCACAGA TACTGTCATC TCCTCTCCAG TTGATTACAA AGTGCACAAA 
GAGCTGACGC ATTGGCACAA CAAAAAGCCA ATTGACCCTA AGCAGCCCAC CAAGGCTCCT 
GACTTTTGGG CAAGACGGCG AAACCAACGC TTCATGGCCG ACATCATTGC CTACTCTGCT 
AGTCTTACCG GTGCATCTGG AAAGATTATC GAACCAGAAA TCGTTGTTGT ACGCGGCTCG 
GTCGAAAGAA GCAAGCCCGT TAGTAGACAT GCGTCGAACA AGGGCAAGAA ACAGGCAAAT 
TCCAACATAC ATCCGGAGAT AGGCGGCGCG CAATACGCTC ATCAAGAGGT AGAACGGCGC 
AAGGAAGCCA AGTTCAAGGT CAAATCGCTG GCAATCATCA CCTCCTGGGA ACAAAGGTGT 
CTCGAGTTCT CAAAGGAGGC ATCTCTGGTT AAGCGATATT TGCTGGTCGA AAAGTATCTC 
TTGGGCTTGA GTCCAGACCA TTTGTCTGTG ATTGGGGCGG AAGTCTCCTT GTATCTTTGC 
CATATTCTAC GCCAAATCCA GGGTCGCTAC AACCCGGACA CGCTGACTCA CTGCCATGTT 
TTGGCCATGC TTTGGTCCCG AACTCAGGAG ACCGCCAAAA TGACCATGAC CAAAGAAGTC 
CACACACTTG TGAACAGAGT TTCAAAGTCT CTCGAACTCC CCCATTACAA GATCCCGGCA 
CTCCTTTCAG AGAGAAAGCT GCCATTTGAG TTCCTGGATA TTGCGAGCGG GGGTTTACTG 
CCTCCTGGAA AGTCTTCGCT CGACTTCCAG CTCGGATTTT GTGGACCGTT CTTTGAACGA 
AGTTTCGACT CGGCCCCAGA TTCTCGTGTG CCTTTTGAGC CCGATGCCTG GCAGAGAAAG 
GTACTTGACG CTATCGATGA GAACAAGAGT CTGCTCGTGA TTGCCCCAAC GTCGGCTGGC 
AAGACGTTCA TCTCATTCTA CGCTATGAGG AAGGTTCTCG AAGCCAACAA CGACGATGTA 
CTGGTGTACA TTGCCCCGAC CAAAGCTCTC GTCAACCAAA TTGCCGCCGA GATCCAAGCC 
CGATTCTCTA AGCAATATGG CCAAGAAGCG CGGTCTGTCT GGGCTATCCA TACTAGGGAT 
TACCGCATCA ATAATGTGAA GGGATGCCAA ATTCTTGTAA CCGTCCCGGA TATCTTGCAG 
ATTCTTCTGC TGGCTCCTTC CAATGCAGCA GGCCCAACGT CTTTTTCCCG GAGAATAAAG 
AGAATCATTT TCGATGAGGT TCATTGTATC GGCCAGTCTG AAGACGGGAT TGTTTGGGAG 
CAGCTTTTGC TGCTTGCACC ATGTCCCATC ATTGCGCTAT CGGCCACCGT GGGAAACCCC 
CTCGAATTCC GAGACTGGCT TTCCAATGCA CAGAAGGCCA AGGGATTCGA TATGGAGATG 
GTGGTACATT CATCCAGGTA CTCAGACCTC CGCAAGTTCA TCCATGATCC GTCTCCCGGT 
TCATATGAAT TCGAAGGCCT CAAACCCGTT GAACGTCTCC CATTTCCTGG ACTAGACTCT 
GATTGCGAGA ATCCGTTGCC ATTCTCGTTT ATACACCCAA TCGCAGCTAT CATGGATAGG 
AGCCGGGACA CGCTTAACGA TGCCAGTCTT GAACCTCGAG ACTGTATCGT GCTTTGGAAG 
CACATGACCA AACATCAGAT TGGCCAGTAT CAAGTTCCAA CCTCCCTGGA TCCTAAGAAA 
ATGTTGCCGT CGCTCGTCAA AAAGTCCGAC GTTACGAAAT GGGAGTCGGC TCTCAAGGAC 
GTCTTGGCTG ACTGGATGCT TGACCCCAAC TCCCCGTTCG ACGAACTTCG AAACGATTTG 
CGTGGAGAAC GGTTTGCTCA CCTATCATCA ACGCACAATC AACCCGAATC CATCGAATCC 
CCGCCTCGTC AATCTGGACC TATTGTATCA CGACGTTCCA TATTCTCACT TGTGACTGAT 
CTTAGGTCGC ATGGAGCATT GCCTGCCATC ATCTTTAATT ACGACCGGGT TGGCTGCGAG 
ACTGTTCTTC GAGAACTCTA CGAGACTCTT AAGTCCGCGG AAGCAGCATA CAAGCAACAC 
GATTCAAAGT GGCTTAAAAA GCTGGCGGGC TTCGAAGAAT GGAAGAAACG CCGTGAGAGC 
GCAAAAACGA AGGACACAAA GATGAAGCAA ACCAAGAAGA CCGGCTTGAG AAATGAGGAC 
GACGATGGCG CAATGAGTAA AGGAGACCGA GCCCGTGACG CAGCAAATCG AGAACTGAGC 
GTTTGGGACA GCTTTGACCC AGAAGCCCCC CTTCAGCAAT TCAGCTTTGC TGATAACACC 
AAACTGACAC AGGAAGAGCT GAATACGCGA TTGAAGTCAC TCAGGGCGGA TGCCGTCCGT 
CCTTGGATCC GAGATGCTCT CTTCCGAGGA ATCGGCGTAC ACCATGCAGG GATGAACCGC 
CAATATAGAC AGATAGTCGA AATGCTTTTC CGTAAAGGTT ATTTGACTGT GGTCGTCGCG 
ACCGGGACAC TTGCCATGGG TCTCAACATG CCTTGTAAAA CAGTTGTCTT TACCGGCGAT 
TCAGTTTTCC TGACTGCTCT CAACTACCGT CAAGCCTCGG GTCGTGCTGG CCGGCGTGGC 
TTCGACCTCC TTGGAAACGT TGTCTTTCAT GGCCTCCATC CGCACCGGGT TTTTGAAATA 
ATGTCTGCGA GGCTCCCGGA TCTTCGCGGG CAGTTTCCAA CATCGGTCAC GCTCATCCTA 
CGGCTCTTCA TCCTTCTACA CGGAACGCAG AACTCTGAGT TTGCAGCCAA CGCTGTAAAG 
GGCCTGCTGA CCCAAAGCAG ACTTTGCTTG GGTGGACCTG ATGCGAAAAT GTCGATCGCA 
CATCATCTTA GATTTTCGAT TGACTACCTC AGACGCCAAC ACCTGTTATC CGAAAATGGT 
GTCCCTCTTA ACTTCTCTGG CCTTGTGGGA CATCTGTACT ACACAGAGAA TGCTGTCTTT 
GCATTCCATG CCCTCCTCAA GGACGGATAC TTTCACGACC TCAGTACTAG CAACCGAAGC 
CGGGAAGACG TCATTCTTGA GATTATGCTT GTTCTAAGTC ACTTGTTCTG TCGCCACGAT 
TTGCCCCAAC ACAAAGACAA GCAGTTCCTC GAGCGTGTAC ATCGCTCTCC GTGTGTTGGA 
ATTCTCCCAG ATCTCCCCCA GAAAGCAAGT GAGGTCCTGG AGCGCCACAA CCAGCAGACC 
TTGTCCATTT TCCAGAACTA TGTCAGCTCT TATACCAATC AACATCTGAC CGGGATGCCG 
GACAACTGTC TCCCCTATAC CAAGTACAAG GTTGATCCTG TCGATGCTCA ACCAACGCCG 
GACCTTTCAA CTGTACTTCC CTCCTCTGAC CGACCTCGGC CGCCCACGGT TGTTCGATCT 
CCATTCTCTG CACTCTCTGG CTTTACGGAC GAATTTGCCA CTATCCACGA GCTTTGCGAG 
ACTGTCCGCG CAGGCGTCTT CCTTGAAGAG TCTGCAGTCC CGTATATTCC GATGGCACCA 
AAGGAAACGA ATGGAGTACC TTGGAACGCC TACCTTTATG ACTTCTTCAA GCATGGAGAT 
ATGGAAGCAC TCAAGACTGT CAACATGATC AAGGGTGGCG ATATCTGGTA CCACCTCAAA 
GACTTCTCGC TCATTCTTTC TTCAATTGTG ACCAGCCTTG CCAATTTTCT GGAGCTTCCA 
AATGCCGACG GTGATTTCGG TTTTGAGGAG GAGGAGGATG ATGATGAGCT TGAGATGGCA 
GATGATGATA GACCAATCTT TAGCGGGGAG GAGGGGAATG GGATAAGGAA TGATGTCTGC 
GGCGCTCCCG CTCCACCACC GCAGAAGGAA CAGAAGAAGA CCAAGGCGAA AAAGGAAGTG 
GTTGCCGAGT CATGGGATGA CGAGATCAGC GATGAAGACA CAGAGGAGGA CACAAAAACG 
GCCAATCACG GTGGCAATTC GTGGTCTGGA TCAGCCTCTG CTTACCCTCA GGCGCCCAGA 
TGGGCTGACG ACGGCGGTCA GTCGTTGATG GAGGTCTTCC GAAATTTCTC GGTACTTCGA 
CAGGAGTTTG AGGAAAAATT CCGCAAGACA TTTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU04472 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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