nik-2 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	nik-2
Entrez Gene ID	3872764
Full Name	nonidentical kinase-2
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395212.1	XP_011393514.1	two-component histidine kinase CHK-1, variant
XM_011395211.1	XP_011393513.1	two-component histidine kinase CHK-1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	mak1	NP_594687.1
Magnaporthe oryzae (rice blast fungus)	MGG_11882	XP_003709348.1
Neurospora crassa	NCU01833	XP_956617.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nik-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nik-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf10114	XM_011395212.1 Latest version!	Neurospora crassa OR74A two-component histidine kinase CHK-1, variant (nik-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ONf10114	XM_011395211.1 Latest version!	Neurospora crassa OR74A two-component histidine kinase CHK-1 (nik-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nik-2 ( XM_011395212.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393514.1 Neurospora crassa OR74A two-component histidine kinase CHK-1, variant (nik-2), mRNA.

Price & Availability↑

CloneID	ONf10114
Clone ID Related Accession (Same CDS sequence)	XM_011395212.1 , XM_011395211.1
Accession Version	XM_011395212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	two-component histidine kinase CHK-1, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502).

ATGCGATTTT TCAGCCTTCA TCGCCGAAGC CACAGTAAAG AGAAAGAGAA AGAGAAGGAG 
AAGGAGAAGG AGAAAAGAAA GAGCATAACC TTGCCTCTTC TGCGCCCTCG CTCCACCGAA 
AGACCAAGAA CAACCGAATC CACGCTCACA TCCACTACCA CTACGACTCC CGCTCTCACT 
CCTACTCCCA CTCTCACAAT CACAGATACT ACGGCGGCCA CGACGCCTAC GATCACGACC 
GCGAAGCCAG TCACGCCCCC CCGCACGACG ACTCCTCCCA GGACTGTCAT CGAGTCACCC 
GACAGTCACT GTTCTAACCA GTACGCCATT GCGCGATCCC ACACCGGTCC TACCGATGGT 
TATGCCCTGA ATCCTCCAGA GGCTTCTGCG CCGTCTGAAC AGCTTGAACA TCAGGACCAA 
ATGACTGGTG GCGCCAACGG GAGAGGAGGG CTAGGGTCGC TTCAACTTCC AACGCCGCAT 
TTACGGGCTT CCAATCAGCA CACTCTTGCA CCCGTTGTGG ATGATATCCG GTCTCGGGAC 
TCGGTGCGCA AGCCCTCCAG GCTTTCTTCC GAAGTTAGCT TCAACGATAG CCCTTTCAGC 
GAACCGACAT CTGAAGAGAG GGACTTGCAG CAGGTAGAGA ATGGAAAGAG AACAGTCGGG 
GAATCGCAGC GGGGTAAGAT GGATACGTCC GAGGCAAAAC GGCAGCTAAG TCAATACCGT 
TTGCACTTGG CCCGCGAGAT GGAAAAAAGA GAGATTGTTG CGGCAACGGA GAACCGGCTG 
GCCATCCCGG GCCCGGCCCG CGTATCTCCT ATACAGGAGG AGGCCAGCAG TCCCTCGCCA 
CAGCTCCCCC AGAGCGGCAG CGAGCCTACC ACGACATCGA CCGAGTCTGG GAATACCATC 
CGTGGCGGTG TCACCCCCGG CTTGACGGCT AGAACTCCCT CATACCCCTT CCCGCGCATG 
GCATCCTCGC CCGGCTTTCC ACACAGTGGC CTGCCATTTC ACCGCCCGTT TACCACGCTC 
TCACCAACTA CATTTCCGAC TACGCATTCG TCTACGGGCG CTCACGTCTC GTTTGCCGGC 
ATTCAGGGTC TTGACCATTC TGTCTCTAAC CCCTCTACTC CTGCCTCAGC AATGACCTTT 
CTGCCCCCAG GGTGCTCCGG TACTGTGCAA GGTGAATTTG CGACACCCAA TCTATACGAC 
CTGACCCTCA TGCTTTCGGC TGAGCCGGGC TTGGATGCAT GGTGGCACAC AGTTGTTCAG 
ATCATGACTG AAGTGTACAG GGCTGAAAGG GTAACCCTGG CTGTGCCTGC CGATCCGACG 
GATATTGAGA ACGTGCCATG GGGCCAAAAG GCAACGTACA ACCCCCACCA GGAGGATGGC 
CTGAGCCTAG GCTATCTTGC TAGAGGCTCG AGCTTTGTCC ACAGCAGTAC CGACGATATC 
ACCGACCTCT ATCAGAACAA CGACGAATCT CTCAAGCCCA AGGGCTTAGC GCGCCCCGGC 
CTGCCGAGCC GACACTCGTT TTCTTCTTAT GAAGACAGCA AGGACAAGAA GGAGTCGATT 
GAACGAACGC CAGTGACACC CCTTCGGCCA CCAATGGCAT CGCGAAGCAA GACTGCTCAC 
CCAGGAGTCG GACGCGATCG AGATGTGAGT GAGGATGATG ATGTTGCTGT GAGCTTGAAT 
CGTGAAGCTT TGGCAGAGCA TGACGCCCTT GAGGGCCAGC AGCCCATCCC AAGCTGGGAA 
GCACCAATAA CCAATAAGAG AGAAGGGCAC GGGAAGCTCT TGAATGTTCT CCAGGCATTG 
GACTACGAAG CTGATCCATT AATCGATCAC AATGGGGTAT TGCGGGTTTT GGAACGTGGT 
CGTGTTGTTG CTCTTACTAG GTCATACCCG TATCTTACAC AAACATCGTC AACCGACAGC 
AAAGCCGCCG ATCCCAGAGT TGCGACGCGC AGCCAGTCGC CAGAGGGGCC ACGGAAAAAG 
ATCAAGAAAA TTCGCACGCG ATCCGAGTCA GCGACTAAAC TCTCTGCGCT TCTGGGCAGT 
GCGACTTTGT CAACCGCCGC AGGCAGGACC TCGGGCGGGC ACGGCTTGGA GAAGAAAGCT 
ACCGGAATTG CCTCTCGCTT GGATGACGAA TACCCAAGAC CCCCGACACC CAAGTATGAG 
GAGTACGAGC AGGCACCACC TTCTCCGTGG TCTCAATCCC CGGCCCCGTC CCCCGCTGTG 
AGGGCTGACC CTAGCGAAAA TCCGTTCTTT GCGGATGCCA TGGTCGATGA GGAGTCGTTT 
AATCCAGGCT CGGCTCCTCC TGACTACTCA GCTCTGCAAC CACCTGAAGC TATTGGGGTT 
GATAACTCGT GGACGGTGTT GCATATCCCT TTAACCCATG TCCTACTGTC GAAACCTCAG 
CAGCCAGGCT TTAAGCTGGA TTCGACGTTT TTGGAGCAAA AGATGTCACA ACGGGGCAAG 
TCAAGTCACA CGCCGACAAT TCCTACTCCC TACCAAGAAC CTGTCGCCAA GGAAAAGCAC 
GCTCCGATTG CGATTCTGTC TATACTCAGC CCCATTATCC CGTATCCCAC CAATCTACGC 
CACTCGTTGG AGCACTTGGC ACCACATCTG GCCACATCTT TTTCTCTGTG TCGTCACTAT 
ACTAACCTCG AAACGGAGCT GAACGGTCTC CAGAAACGAA GGCCACAGAC GGTTGGCTTT 
GGTGCGCTGG GTCCTGACGG ACGTCCGCTG GCTGATCCTA CCGCTTTCAC AATGGGATAC 
CTCCCAACCG ATGACAGTAT CTCTCAGCAG TCGCACGCTG GCAGCATAAC CAGTCCTAGC 
GAGTACTCTG GTCCCTCTCG CAGTGTCATC CACTCTCCGA ATGGGACGCC TACTTGGGAT 
TCCTCGGCTC TGAGTTATCT AATGGGCAAG AGGTCATCCT CTGCCAGCCC AGCTCCTGTC 
GCCGCTGGGG ACAGTTACTT CACCACCACG CCAAAGCCGG GAGGTACTGG TGTGGGACAA 
CGAACCAAGA CAGGATCACG AGAAAGTACA ACCCTTGAAA GGAAGGGTTC CTTGAAGATA 
CCCGGAGGGA AGTCTCAGTG GCAGGATGCA GCTGGTGTCG CTCCCGTGTC GCAGGAGTCT 
CAGGGAAGCG GAGAAAGCAA CCGCCGGAGT ATTGATGAAC CTTCGCCGAC AAACTCACAA 
CAGAAGACTG CCGTTCTCAA AGAGCAGCGG GAAGAAAGAG GCGGCATCGC AAGCAGTCAG 
CCAGTCGAGA CTCAACGGAG AAAGCCTGCT GGTACTCCCA CAGGCCAGCA TCGGCACACG 
CAGTTGCACT CATATGGTGC CGACTTTGCC ACCACATTCC AGTCCCTTCC TCCGAGCTCC 
ACCGTCGGCA AAGGTATAAA CCAGGGCGCA ACGGCATCTA GGCAAGGATC GATCTCATCG 
ACAGACATGA CACCACCATC GGACAAGCTC AAAGGCCTTA TCCTTGACTC TTTGCCCGCT 
CATGTCTTCG TAGCCCTTCC ACAAACGGGT GAAATCGTCT GGGTGAACAG TCGGTTCTTG 
ACATACCGCG GGCAAACCTC TAGCGATTTG GCCGCAGACC CTTGGGGGAG CATCCACCCT 
GAAGACAGAG AAGAGTACCT TCGTAAATGG AGTGCGTCTG TCCGTACCGG GGAACCGCTC 
TCAGAGACCG TAAGAATCCG TCGCTTCGAC GGGGCTTATC GGTGGTTCCA AGCGCGGGCT 
GTCGCCTCCA AGGACAAGCG GGGTGTTATC ATGCAGTTCC TGGGTTCATA TATGGATATC 
CATGATAAGC ATGTCGCACA GCTCAGAGCA GCACGGCAGG AAGAGATCGA GGTATCGGAG 
GCAAAGCATC GTCTGCTTGC TAACTTAATC CCCCAGATCA TCTTCACTGC AACTGAAGAC 
GAGGGTATCA CTTTCGCGAA CGAGCAATGG CTTTCTTATA CCGGACAAGG TTTTGATGAT 
GTTCTTGGAC TTGGCTTCAT GGATTTCGTG CACCCAGAGG ATCTGGCTAA ATGCCGGATT 
CCCCTCGAAC GACCCACCTC ACCACATGTC AGGCAGGACA AGACAAAGCA TGACCATTCG 
CCTTCGCCCA GCAAGTTTGG CATGGCCGCT ATGGGCAAAC AGCCTGCTAC CCCTCACGCT 
GAAAATGTGT CTGGGCCCGT ACACCCAGCT CTCACCCGAC ACAGCAGTAG CACCAGCAGT 
TCTGCTAACG ATCTCGCCGG TCCTAACCTG TCCGAGCTTG CCAGGCAGGG AGTCATCAAA 
ATCAGCACCG ACAGCAAGGG CCAGCTCTCA TACACAACAG AGATCCGACT ACGTTCCAAG 
ACCGGCGAGT ATCGCTGGCA TCTCATTCGA TGCGTTGAGA TTGATAATGT CGACTTTGGT 
AATGGCGTCA GCTCTTATTT CGGCTCTGCC ACCGACATCA ACGATCTGAA ACTCCTGGAA 
ACCAAGTTAA AGGAGGCTAT GGAGTCGAAG AGCCGTTTCC TTAGCAACAT GTCGCATGAG 
ATCCGAACCC CTCTTATTGG CATTTCTGGT ATGGTCAGTT TCCTTCAGGA TACCACACTC 
AACGAGGAGC AACGTGATTA CACGAATACC ATTCAGACCA GCGCCAACAG TCTGATCATG 
ATCATCAACG ATATCCTGGA TCTTTCCAAG GTTGATGCAG GCATGATGAA GTTGAACTAC 
GAATGGTTTC ACACGCGCTC TCTCATCGAG GATGTCAACG AGCTGGTGTC CACCATGGCG 
ATCGCCAAGC GTCTCGAGCT CAACTATGTT GTCGAAGAAG ACGTGCCTGC ATGGGTCAAG 
GGTGATAAGG TCCGAATCCG CCAGGTCTTG CTCAACGTCA TCGGCAATGC CATCAAGTTC 
ACTAATGAAG GAGAGGTATT CAGCCGCTGC AAGGTTGTTG TTCCCAACCA TGGCCATTTG 
TACGAGAACG AGATTATGCT TGAGTTCTCC ATCATTGACA CTGGAAGAGG CTTCACCGAA 
GAGGAAGCCG AGTTGATCTT CAAACCCTTC AGCCAGATTG ATGGAAGCAG TACCCGCCAA 
CACGGTGGAA GTGGCCTAGG TTTGGTCATT TCGCGCCAAC TGGTCGAGCT ACACGGCGGT 
AAGATGGACG GTAAGGCGGT GCTAGGAAAG GGATCAACGT TCACCTTTAC CGCAAGGTTT 
GGGCTTCCCA CAGAATCCGA CAACCCTGAC CTGGCTGCAC CACCTGCGCC TACGCCAGGC 
CTTATACCGG AAATGAACAT TCTCCGTCGG TCCTCCATGG CATCCCCTCA CAGTAGGCCA 
TATCTCACTT CTGCACCTTC ATCGGGCTCA GTGGTACCTG TTGAGTCTGA CACTAGATCG 
CCGGCAGCAG TCTCCACCGG TAGCTCGGAA CCGTCTATCC ACTCGTCACG CACCCGAAAC 
ACTGAACGCT CATCCATATC GTCTGTCAAC GTCGGGTCCT TAGCACGATT TAGCGAGGCT 
GCCCGTGCTA GTGGGCACGA TTTGTCCCAG ATGAAGCTTG AAATGCCTCA TGGCAGAGGA 
GGAGGTGGCT CCGACGCGGG CACGACCCCA ACGCCTAGCA ACTCCAGCAA CGAGCGCCTG 
TCGAGGCCTA CCATTAAAGA GGGCGGTGGT TCAGAAGATA GCGATGGGTA TCACACATCC 
CTATTTTCCA TCTTGATTAT CTGCCCGCAA ACGCACAGCC GCGAAGCCAC GACGAAGCAT 
ATCGAGATGA CGCTGCCAAA GAGTAACCCT CACCAGATTA CAGCGGTCGC ATCTGTTCAG 
GAGGCGCGCA AGCTGATCGG TGGTGAGGAC CCCGTCTTCT TCACGCACGT GGTGGTTAAC 
CTTCCGACAC CTGAGCAGAT CATTGAGTTG ATGGATGAAG TCCAGCGCTC AACCATGATT 
GGCCGCGGCG TTACCATCCT CATTCTCTCC GACTCGGTAC AGCGACAGGC GGTTCTGAAG 
CAGGTAGCCG GTACCCAGTA TGAGGACATT TTGTCTGAAA ACCGGGTGAC CTATATCTAC 
AAACCTGTCA AGCCTTCACG GTTCGCGGTG ATCTTTGATC CGGCCAAGGA GCGTGATCTC 
AGCATCGACC GGAACAGATC AACGGCAGAG CAGTTAGTGG AAAACCAAAA GCAGAGTTAC 
CTTGAGGTGG AGAAGCGCAT GGGCAACAAG GGGTACAAAG TCCTCTTAGT TGAGGACAAC 
TTGGTCAACC AAAAGGTGCT GCAAAAGTAT CTTAAGAAGG TCGGCGTGGA CGTGGAGTTG 
GTCGCAGACG GAGTAGAGTG CACTGATGTC GTGTTTTCGC GGCCTCATGA CTACTTTTCG 
TTGATCTTGT GCGATCTCCA CATGCCACGC AAGGACGGAT ATCAAGCCTG CCGTGAAATT 
CGTGAGTGGG AAGCCAAGGG CAACTTTCCT GGAAGGGCGT TGCCCATCAT TGCCCTGTCG 
GCAAACGTCA TGAGTGACGT CCAGGATAAG TGTCTCCAAG CAGGGTTCAA CGACTACGTA 
ACGAAACCTG TCGACTTTAT CAATCTGAGT AATGCTCTTT CGAGGTTCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393514.1
CDS	928..7461
Translation	MRFFSLHRRSHSKEKEKEKEKEKEKRKSITLPLLRPRSTERPRTTESTLTSTTTTTPALTPTPTLTITDTTAATTPTITTAKPVTPPRTTTPPRTVIESPDSHCSNQYAIARSHTGPTDGYALNPPEASAPSEQLEHQDQMTGGANGRGGLGSLQLPTPHLRASNQHTLAPVVDDIRSRDSVRKPSRLSSEVSFNDSPFSEPTSEERDLQQVENGKRTVGESQRGKMDTSEAKRQLSQYRLHLAREMEKREIVAATENRLAIPGPARVSPIQEEASSPSPQLPQSGSEPTTTSTESGNTIRGGVTPGLTARTPSYPFPRMASSPGFPHSGLPFHRPFTTLSPTTFPTTHSSTGAHVSFAGIQGLDHSVSNPSTPASAMTFLPPGCSGTVQGEFATPNLYDLTLMLSAEPGLDAWWHTVVQIMTEVYRAERVTLAVPADPTDIENVPWGQKATYNPHQEDGLSLGYLARGSSFVHSSTDDITDLYQNNDESLKPKGLARPGLPSRHSFSSYEDSKDKKESIERTPVTPLRPPMASRSKTAHPGVGRDRDVSEDDDVAVSLNREALAEHDALEGQQPIPSWEAPITNKREGHGKLLNVLQALDYEADPLIDHNGVLRVLERGRVVALTRSYPYLTQTSSTDSKAADPRVATRSQSPEGPRKKIKKIRTRSESATKLSALLGSATLSTAAGRTSGGHGLEKKATGIASRLDDEYPRPPTPKYEEYEQAPPSPWSQSPAPSPAVRADPSENPFFADAMVDEESFNPGSAPPDYSALQPPEAIGVDNSWTVLHIPLTHVLLSKPQQPGFKLDSTFLEQKMSQRGKSSHTPTIPTPYQEPVAKEKHAPIAILSILSPIIPYPTNLRHSLEHLAPHLATSFSLCRHYTNLETELNGLQKRRPQTVGFGALGPDGRPLADPTAFTMGYLPTDDSISQQSHAGSITSPSEYSGPSRSVIHSPNGTPTWDSSALSYLMGKRSSSASPAPVAAGDSYFTTTPKPGGTGVGQRTKTGSRESTTLERKGSLKIPGGKSQWQDAAGVAPVSQESQGSGESNRRSIDEPSPTNSQQKTAVLKEQREERGGIASSQPVETQRRKPAGTPTGQHRHTQLHSYGADFATTFQSLPPSSTVGKGINQGATASRQGSISSTDMTPPSDKLKGLILDSLPAHVFVALPQTGEIVWVNSRFLTYRGQTSSDLAADPWGSIHPEDREEYLRKWSASVRTGEPLSETVRIRRFDGAYRWFQARAVASKDKRGVIMQFLGSYMDIHDKHVAQLRAARQEEIEVSEAKHRLLANLIPQIIFTATEDEGITFANEQWLSYTGQGFDDVLGLGFMDFVHPEDLAKCRIPLERPTSPHVRQDKTKHDHSPSPSKFGMAAMGKQPATPHAENVSGPVHPALTRHSSSTSSSANDLAGPNLSELARQGVIKISTDSKGQLSYTTEIRLRSKTGEYRWHLIRCVEIDNVDFGNGVSSYFGSATDINDLKLLETKLKEAMESKSRFLSNMSHEIRTPLIGISGMVSFLQDTTLNEEQRDYTNTIQTSANSLIMIINDILDLSKVDAGMMKLNYEWFHTRSLIEDVNELVSTMAIAKRLELNYVVEEDVPAWVKGDKVRIRQVLLNVIGNAIKFTNEGEVFSRCKVVVPNHGHLYENEIMLEFSIIDTGRGFTEEEAELIFKPFSQIDGSSTRQHGGSGLGLVISRQLVELHGGKMDGKAVLGKGSTFTFTARFGLPTESDNPDLAAPPAPTPGLIPEMNILRRSSMASPHSRPYLTSAPSSGSVVPVESDTRSPAAVSTGSSEPSIHSSRTRNTERSSISSVNVGSLARFSEAARASGHDLSQMKLEMPHGRGGGGSDAGTTPTPSNSSNERLSRPTIKEGGGSEDSDGYHTSLFSILIICPQTHSREATTKHIEMTLPKSNPHQITAVASVQEARKLIGGEDPVFFTHVVVNLPTPEQIIELMDEVQRSTMIGRGVTILILSDSVQRQAVLKQVAGTQYEDILSENRVTYIYKPVKPSRFAVIFDPAKERDLSIDRNRSTAEQLVENQKQSYLEVEKRMGNKGYKVLLVEDNLVNQKVLQKYLKKVGVDVELVADGVECTDVVFSRPHDYFSLILCDLHMPRKDGYQACREIREWEAKGNFPGRALPIIALSANVMSDVQDKCLQAGFNDYVTKPVDFINLSNALSRFF

Target ORF information:

RefSeq Version	XM_011395212.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A two-component histidine kinase CHK-1, variant (nik-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395212.1

ORF Insert Sequence:

ATGCGATTTT TCAGCCTTCA TCGCCGAAGC CACAGTAAAG AGAAAGAGAA AGAGAAGGAG 
AAGGAGAAGG AGAAAAGAAA GAGCATAACC TTGCCTCTTC TGCGCCCTCG CTCCACCGAA 
AGACCAAGAA CAACCGAATC CACGCTCACA TCCACTACCA CTACGACTCC CGCTCTCACT 
CCTACTCCCA CTCTCACAAT CACAGATACT ACGGCGGCCA CGACGCCTAC GATCACGACC 
GCGAAGCCAG TCACGCCCCC CCGCACGACG ACTCCTCCCA GGACTGTCAT CGAGTCACCC 
GACAGTCACT GTTCTAACCA GTACGCCATT GCGCGATCCC ACACCGGTCC TACCGATGGT 
TATGCCCTGA ATCCTCCAGA GGCTTCTGCG CCGTCTGAAC AGCTTGAACA TCAGGACCAA 
ATGACTGGTG GCGCCAACGG GAGAGGAGGG CTAGGGTCGC TTCAACTTCC AACGCCGCAT 
TTACGGGCTT CCAATCAGCA CACTCTTGCA CCCGTTGTGG ATGATATCCG GTCTCGGGAC 
TCGGTGCGCA AGCCCTCCAG GCTTTCTTCC GAAGTTAGCT TCAACGATAG CCCTTTCAGC 
GAACCGACAT CTGAAGAGAG GGACTTGCAG CAGGTAGAGA ATGGAAAGAG AACAGTCGGG 
GAATCGCAGC GGGGTAAGAT GGATACGTCC GAGGCAAAAC GGCAGCTAAG TCAATACCGT 
TTGCACTTGG CCCGCGAGAT GGAAAAAAGA GAGATTGTTG CGGCAACGGA GAACCGGCTG 
GCCATCCCGG GCCCGGCCCG CGTATCTCCT ATACAGGAGG AGGCCAGCAG TCCCTCGCCA 
CAGCTCCCCC AGAGCGGCAG CGAGCCTACC ACGACATCGA CCGAGTCTGG GAATACCATC 
CGTGGCGGTG TCACCCCCGG CTTGACGGCT AGAACTCCCT CATACCCCTT CCCGCGCATG 
GCATCCTCGC CCGGCTTTCC ACACAGTGGC CTGCCATTTC ACCGCCCGTT TACCACGCTC 
TCACCAACTA CATTTCCGAC TACGCATTCG TCTACGGGCG CTCACGTCTC GTTTGCCGGC 
ATTCAGGGTC TTGACCATTC TGTCTCTAAC CCCTCTACTC CTGCCTCAGC AATGACCTTT 
CTGCCCCCAG GGTGCTCCGG TACTGTGCAA GGTGAATTTG CGACACCCAA TCTATACGAC 
CTGACCCTCA TGCTTTCGGC TGAGCCGGGC TTGGATGCAT GGTGGCACAC AGTTGTTCAG 
ATCATGACTG AAGTGTACAG GGCTGAAAGG GTAACCCTGG CTGTGCCTGC CGATCCGACG 
GATATTGAGA ACGTGCCATG GGGCCAAAAG GCAACGTACA ACCCCCACCA GGAGGATGGC 
CTGAGCCTAG GCTATCTTGC TAGAGGCTCG AGCTTTGTCC ACAGCAGTAC CGACGATATC 
ACCGACCTCT ATCAGAACAA CGACGAATCT CTCAAGCCCA AGGGCTTAGC GCGCCCCGGC 
CTGCCGAGCC GACACTCGTT TTCTTCTTAT GAAGACAGCA AGGACAAGAA GGAGTCGATT 
GAACGAACGC CAGTGACACC CCTTCGGCCA CCAATGGCAT CGCGAAGCAA GACTGCTCAC 
CCAGGAGTCG GACGCGATCG AGATGTGAGT GAGGATGATG ATGTTGCTGT GAGCTTGAAT 
CGTGAAGCTT TGGCAGAGCA TGACGCCCTT GAGGGCCAGC AGCCCATCCC AAGCTGGGAA 
GCACCAATAA CCAATAAGAG AGAAGGGCAC GGGAAGCTCT TGAATGTTCT CCAGGCATTG 
GACTACGAAG CTGATCCATT AATCGATCAC AATGGGGTAT TGCGGGTTTT GGAACGTGGT 
CGTGTTGTTG CTCTTACTAG GTCATACCCG TATCTTACAC AAACATCGTC AACCGACAGC 
AAAGCCGCCG ATCCCAGAGT TGCGACGCGC AGCCAGTCGC CAGAGGGGCC ACGGAAAAAG 
ATCAAGAAAA TTCGCACGCG ATCCGAGTCA GCGACTAAAC TCTCTGCGCT TCTGGGCAGT 
GCGACTTTGT CAACCGCCGC AGGCAGGACC TCGGGCGGGC ACGGCTTGGA GAAGAAAGCT 
ACCGGAATTG CCTCTCGCTT GGATGACGAA TACCCAAGAC CCCCGACACC CAAGTATGAG 
GAGTACGAGC AGGCACCACC TTCTCCGTGG TCTCAATCCC CGGCCCCGTC CCCCGCTGTG 
AGGGCTGACC CTAGCGAAAA TCCGTTCTTT GCGGATGCCA TGGTCGATGA GGAGTCGTTT 
AATCCAGGCT CGGCTCCTCC TGACTACTCA GCTCTGCAAC CACCTGAAGC TATTGGGGTT 
GATAACTCGT GGACGGTGTT GCATATCCCT TTAACCCATG TCCTACTGTC GAAACCTCAG 
CAGCCAGGCT TTAAGCTGGA TTCGACGTTT TTGGAGCAAA AGATGTCACA ACGGGGCAAG 
TCAAGTCACA CGCCGACAAT TCCTACTCCC TACCAAGAAC CTGTCGCCAA GGAAAAGCAC 
GCTCCGATTG CGATTCTGTC TATACTCAGC CCCATTATCC CGTATCCCAC CAATCTACGC 
CACTCGTTGG AGCACTTGGC ACCACATCTG GCCACATCTT TTTCTCTGTG TCGTCACTAT 
ACTAACCTCG AAACGGAGCT GAACGGTCTC CAGAAACGAA GGCCACAGAC GGTTGGCTTT 
GGTGCGCTGG GTCCTGACGG ACGTCCGCTG GCTGATCCTA CCGCTTTCAC AATGGGATAC 
CTCCCAACCG ATGACAGTAT CTCTCAGCAG TCGCACGCTG GCAGCATAAC CAGTCCTAGC 
GAGTACTCTG GTCCCTCTCG CAGTGTCATC CACTCTCCGA ATGGGACGCC TACTTGGGAT 
TCCTCGGCTC TGAGTTATCT AATGGGCAAG AGGTCATCCT CTGCCAGCCC AGCTCCTGTC 
GCCGCTGGGG ACAGTTACTT CACCACCACG CCAAAGCCGG GAGGTACTGG TGTGGGACAA 
CGAACCAAGA CAGGATCACG AGAAAGTACA ACCCTTGAAA GGAAGGGTTC CTTGAAGATA 
CCCGGAGGGA AGTCTCAGTG GCAGGATGCA GCTGGTGTCG CTCCCGTGTC GCAGGAGTCT 
CAGGGAAGCG GAGAAAGCAA CCGCCGGAGT ATTGATGAAC CTTCGCCGAC AAACTCACAA 
CAGAAGACTG CCGTTCTCAA AGAGCAGCGG GAAGAAAGAG GCGGCATCGC AAGCAGTCAG 
CCAGTCGAGA CTCAACGGAG AAAGCCTGCT GGTACTCCCA CAGGCCAGCA TCGGCACACG 
CAGTTGCACT CATATGGTGC CGACTTTGCC ACCACATTCC AGTCCCTTCC TCCGAGCTCC 
ACCGTCGGCA AAGGTATAAA CCAGGGCGCA ACGGCATCTA GGCAAGGATC GATCTCATCG 
ACAGACATGA CACCACCATC GGACAAGCTC AAAGGCCTTA TCCTTGACTC TTTGCCCGCT 
CATGTCTTCG TAGCCCTTCC ACAAACGGGT GAAATCGTCT GGGTGAACAG TCGGTTCTTG 
ACATACCGCG GGCAAACCTC TAGCGATTTG GCCGCAGACC CTTGGGGGAG CATCCACCCT 
GAAGACAGAG AAGAGTACCT TCGTAAATGG AGTGCGTCTG TCCGTACCGG GGAACCGCTC 
TCAGAGACCG TAAGAATCCG TCGCTTCGAC GGGGCTTATC GGTGGTTCCA AGCGCGGGCT 
GTCGCCTCCA AGGACAAGCG GGGTGTTATC ATGCAGTTCC TGGGTTCATA TATGGATATC 
CATGATAAGC ATGTCGCACA GCTCAGAGCA GCACGGCAGG AAGAGATCGA GGTATCGGAG 
GCAAAGCATC GTCTGCTTGC TAACTTAATC CCCCAGATCA TCTTCACTGC AACTGAAGAC 
GAGGGTATCA CTTTCGCGAA CGAGCAATGG CTTTCTTATA CCGGACAAGG TTTTGATGAT 
GTTCTTGGAC TTGGCTTCAT GGATTTCGTG CACCCAGAGG ATCTGGCTAA ATGCCGGATT 
CCCCTCGAAC GACCCACCTC ACCACATGTC AGGCAGGACA AGACAAAGCA TGACCATTCG 
CCTTCGCCCA GCAAGTTTGG CATGGCCGCT ATGGGCAAAC AGCCTGCTAC CCCTCACGCT 
GAAAATGTGT CTGGGCCCGT ACACCCAGCT CTCACCCGAC ACAGCAGTAG CACCAGCAGT 
TCTGCTAACG ATCTCGCCGG TCCTAACCTG TCCGAGCTTG CCAGGCAGGG AGTCATCAAA 
ATCAGCACCG ACAGCAAGGG CCAGCTCTCA TACACAACAG AGATCCGACT ACGTTCCAAG 
ACCGGCGAGT ATCGCTGGCA TCTCATTCGA TGCGTTGAGA TTGATAATGT CGACTTTGGT 
AATGGCGTCA GCTCTTATTT CGGCTCTGCC ACCGACATCA ACGATCTGAA ACTCCTGGAA 
ACCAAGTTAA AGGAGGCTAT GGAGTCGAAG AGCCGTTTCC TTAGCAACAT GTCGCATGAG 
ATCCGAACCC CTCTTATTGG CATTTCTGGT ATGGTCAGTT TCCTTCAGGA TACCACACTC 
AACGAGGAGC AACGTGATTA CACGAATACC ATTCAGACCA GCGCCAACAG TCTGATCATG 
ATCATCAACG ATATCCTGGA TCTTTCCAAG GTTGATGCAG GCATGATGAA GTTGAACTAC 
GAATGGTTTC ACACGCGCTC TCTCATCGAG GATGTCAACG AGCTGGTGTC CACCATGGCG 
ATCGCCAAGC GTCTCGAGCT CAACTATGTT GTCGAAGAAG ACGTGCCTGC ATGGGTCAAG 
GGTGATAAGG TCCGAATCCG CCAGGTCTTG CTCAACGTCA TCGGCAATGC CATCAAGTTC 
ACTAATGAAG GAGAGGTATT CAGCCGCTGC AAGGTTGTTG TTCCCAACCA TGGCCATTTG 
TACGAGAACG AGATTATGCT TGAGTTCTCC ATCATTGACA CTGGAAGAGG CTTCACCGAA 
GAGGAAGCCG AGTTGATCTT CAAACCCTTC AGCCAGATTG ATGGAAGCAG TACCCGCCAA 
CACGGTGGAA GTGGCCTAGG TTTGGTCATT TCGCGCCAAC TGGTCGAGCT ACACGGCGGT 
AAGATGGACG GTAAGGCGGT GCTAGGAAAG GGATCAACGT TCACCTTTAC CGCAAGGTTT 
GGGCTTCCCA CAGAATCCGA CAACCCTGAC CTGGCTGCAC CACCTGCGCC TACGCCAGGC 
CTTATACCGG AAATGAACAT TCTCCGTCGG TCCTCCATGG CATCCCCTCA CAGTAGGCCA 
TATCTCACTT CTGCACCTTC ATCGGGCTCA GTGGTACCTG TTGAGTCTGA CACTAGATCG 
CCGGCAGCAG TCTCCACCGG TAGCTCGGAA CCGTCTATCC ACTCGTCACG CACCCGAAAC 
ACTGAACGCT CATCCATATC GTCTGTCAAC GTCGGGTCCT TAGCACGATT TAGCGAGGCT 
GCCCGTGCTA GTGGGCACGA TTTGTCCCAG ATGAAGCTTG AAATGCCTCA TGGCAGAGGA 
GGAGGTGGCT CCGACGCGGG CACGACCCCA ACGCCTAGCA ACTCCAGCAA CGAGCGCCTG 
TCGAGGCCTA CCATTAAAGA GGGCGGTGGT TCAGAAGATA GCGATGGGTA TCACACATCC 
CTATTTTCCA TCTTGATTAT CTGCCCGCAA ACGCACAGCC GCGAAGCCAC GACGAAGCAT 
ATCGAGATGA CGCTGCCAAA GAGTAACCCT CACCAGATTA CAGCGGTCGC ATCTGTTCAG 
GAGGCGCGCA AGCTGATCGG TGGTGAGGAC CCCGTCTTCT TCACGCACGT GGTGGTTAAC 
CTTCCGACAC CTGAGCAGAT CATTGAGTTG ATGGATGAAG TCCAGCGCTC AACCATGATT 
GGCCGCGGCG TTACCATCCT CATTCTCTCC GACTCGGTAC AGCGACAGGC GGTTCTGAAG 
CAGGTAGCCG GTACCCAGTA TGAGGACATT TTGTCTGAAA ACCGGGTGAC CTATATCTAC 
AAACCTGTCA AGCCTTCACG GTTCGCGGTG ATCTTTGATC CGGCCAAGGA GCGTGATCTC 
AGCATCGACC GGAACAGATC AACGGCAGAG CAGTTAGTGG AAAACCAAAA GCAGAGTTAC 
CTTGAGGTGG AGAAGCGCAT GGGCAACAAG GGGTACAAAG TCCTCTTAGT TGAGGACAAC 
TTGGTCAACC AAAAGGTGCT GCAAAAGTAT CTTAAGAAGG TCGGCGTGGA CGTGGAGTTG 
GTCGCAGACG GAGTAGAGTG CACTGATGTC GTGTTTTCGC GGCCTCATGA CTACTTTTCG 
TTGATCTTGT GCGATCTCCA CATGCCACGC AAGGACGGAT ATCAAGCCTG CCGTGAAATT 
CGTGAGTGGG AAGCCAAGGG CAACTTTCCT GGAAGGGCGT TGCCCATCAT TGCCCTGTCG 
GCAAACGTCA TGAGTGACGT CCAGGATAAG TGTCTCCAAG CAGGGTTCAA CGACTACGTA 
ACGAAACCTG TCGACTTTAT CAATCTGAGT AATGCTCTTT CGAGGTTCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nik-2 ( XM_011395211.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393513.1 Neurospora crassa OR74A two-component histidine kinase CHK-1 (nik-2), mRNA.

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CloneID	ONf10114
Clone ID Related Accession (Same CDS sequence)	XM_011395212.1 , XM_011395211.1
Accession Version	XM_011395211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	two-component histidine kinase CHK-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502).

ATGCGATTTT TCAGCCTTCA TCGCCGAAGC CACAGTAAAG AGAAAGAGAA AGAGAAGGAG 
AAGGAGAAGG AGAAAAGAAA GAGCATAACC TTGCCTCTTC TGCGCCCTCG CTCCACCGAA 
AGACCAAGAA CAACCGAATC CACGCTCACA TCCACTACCA CTACGACTCC CGCTCTCACT 
CCTACTCCCA CTCTCACAAT CACAGATACT ACGGCGGCCA CGACGCCTAC GATCACGACC 
GCGAAGCCAG TCACGCCCCC CCGCACGACG ACTCCTCCCA GGACTGTCAT CGAGTCACCC 
GACAGTCACT GTTCTAACCA GTACGCCATT GCGCGATCCC ACACCGGTCC TACCGATGGT 
TATGCCCTGA ATCCTCCAGA GGCTTCTGCG CCGTCTGAAC AGCTTGAACA TCAGGACCAA 
ATGACTGGTG GCGCCAACGG GAGAGGAGGG CTAGGGTCGC TTCAACTTCC AACGCCGCAT 
TTACGGGCTT CCAATCAGCA CACTCTTGCA CCCGTTGTGG ATGATATCCG GTCTCGGGAC 
TCGGTGCGCA AGCCCTCCAG GCTTTCTTCC GAAGTTAGCT TCAACGATAG CCCTTTCAGC 
GAACCGACAT CTGAAGAGAG GGACTTGCAG CAGGTAGAGA ATGGAAAGAG AACAGTCGGG 
GAATCGCAGC GGGGTAAGAT GGATACGTCC GAGGCAAAAC GGCAGCTAAG TCAATACCGT 
TTGCACTTGG CCCGCGAGAT GGAAAAAAGA GAGATTGTTG CGGCAACGGA GAACCGGCTG 
GCCATCCCGG GCCCGGCCCG CGTATCTCCT ATACAGGAGG AGGCCAGCAG TCCCTCGCCA 
CAGCTCCCCC AGAGCGGCAG CGAGCCTACC ACGACATCGA CCGAGTCTGG GAATACCATC 
CGTGGCGGTG TCACCCCCGG CTTGACGGCT AGAACTCCCT CATACCCCTT CCCGCGCATG 
GCATCCTCGC CCGGCTTTCC ACACAGTGGC CTGCCATTTC ACCGCCCGTT TACCACGCTC 
TCACCAACTA CATTTCCGAC TACGCATTCG TCTACGGGCG CTCACGTCTC GTTTGCCGGC 
ATTCAGGGTC TTGACCATTC TGTCTCTAAC CCCTCTACTC CTGCCTCAGC AATGACCTTT 
CTGCCCCCAG GGTGCTCCGG TACTGTGCAA GGTGAATTTG CGACACCCAA TCTATACGAC 
CTGACCCTCA TGCTTTCGGC TGAGCCGGGC TTGGATGCAT GGTGGCACAC AGTTGTTCAG 
ATCATGACTG AAGTGTACAG GGCTGAAAGG GTAACCCTGG CTGTGCCTGC CGATCCGACG 
GATATTGAGA ACGTGCCATG GGGCCAAAAG GCAACGTACA ACCCCCACCA GGAGGATGGC 
CTGAGCCTAG GCTATCTTGC TAGAGGCTCG AGCTTTGTCC ACAGCAGTAC CGACGATATC 
ACCGACCTCT ATCAGAACAA CGACGAATCT CTCAAGCCCA AGGGCTTAGC GCGCCCCGGC 
CTGCCGAGCC GACACTCGTT TTCTTCTTAT GAAGACAGCA AGGACAAGAA GGAGTCGATT 
GAACGAACGC CAGTGACACC CCTTCGGCCA CCAATGGCAT CGCGAAGCAA GACTGCTCAC 
CCAGGAGTCG GACGCGATCG AGATGTGAGT GAGGATGATG ATGTTGCTGT GAGCTTGAAT 
CGTGAAGCTT TGGCAGAGCA TGACGCCCTT GAGGGCCAGC AGCCCATCCC AAGCTGGGAA 
GCACCAATAA CCAATAAGAG AGAAGGGCAC GGGAAGCTCT TGAATGTTCT CCAGGCATTG 
GACTACGAAG CTGATCCATT AATCGATCAC AATGGGGTAT TGCGGGTTTT GGAACGTGGT 
CGTGTTGTTG CTCTTACTAG GTCATACCCG TATCTTACAC AAACATCGTC AACCGACAGC 
AAAGCCGCCG ATCCCAGAGT TGCGACGCGC AGCCAGTCGC CAGAGGGGCC ACGGAAAAAG 
ATCAAGAAAA TTCGCACGCG ATCCGAGTCA GCGACTAAAC TCTCTGCGCT TCTGGGCAGT 
GCGACTTTGT CAACCGCCGC AGGCAGGACC TCGGGCGGGC ACGGCTTGGA GAAGAAAGCT 
ACCGGAATTG CCTCTCGCTT GGATGACGAA TACCCAAGAC CCCCGACACC CAAGTATGAG 
GAGTACGAGC AGGCACCACC TTCTCCGTGG TCTCAATCCC CGGCCCCGTC CCCCGCTGTG 
AGGGCTGACC CTAGCGAAAA TCCGTTCTTT GCGGATGCCA TGGTCGATGA GGAGTCGTTT 
AATCCAGGCT CGGCTCCTCC TGACTACTCA GCTCTGCAAC CACCTGAAGC TATTGGGGTT 
GATAACTCGT GGACGGTGTT GCATATCCCT TTAACCCATG TCCTACTGTC GAAACCTCAG 
CAGCCAGGCT TTAAGCTGGA TTCGACGTTT TTGGAGCAAA AGATGTCACA ACGGGGCAAG 
TCAAGTCACA CGCCGACAAT TCCTACTCCC TACCAAGAAC CTGTCGCCAA GGAAAAGCAC 
GCTCCGATTG CGATTCTGTC TATACTCAGC CCCATTATCC CGTATCCCAC CAATCTACGC 
CACTCGTTGG AGCACTTGGC ACCACATCTG GCCACATCTT TTTCTCTGTG TCGTCACTAT 
ACTAACCTCG AAACGGAGCT GAACGGTCTC CAGAAACGAA GGCCACAGAC GGTTGGCTTT 
GGTGCGCTGG GTCCTGACGG ACGTCCGCTG GCTGATCCTA CCGCTTTCAC AATGGGATAC 
CTCCCAACCG ATGACAGTAT CTCTCAGCAG TCGCACGCTG GCAGCATAAC CAGTCCTAGC 
GAGTACTCTG GTCCCTCTCG CAGTGTCATC CACTCTCCGA ATGGGACGCC TACTTGGGAT 
TCCTCGGCTC TGAGTTATCT AATGGGCAAG AGGTCATCCT CTGCCAGCCC AGCTCCTGTC 
GCCGCTGGGG ACAGTTACTT CACCACCACG CCAAAGCCGG GAGGTACTGG TGTGGGACAA 
CGAACCAAGA CAGGATCACG AGAAAGTACA ACCCTTGAAA GGAAGGGTTC CTTGAAGATA 
CCCGGAGGGA AGTCTCAGTG GCAGGATGCA GCTGGTGTCG CTCCCGTGTC GCAGGAGTCT 
CAGGGAAGCG GAGAAAGCAA CCGCCGGAGT ATTGATGAAC CTTCGCCGAC AAACTCACAA 
CAGAAGACTG CCGTTCTCAA AGAGCAGCGG GAAGAAAGAG GCGGCATCGC AAGCAGTCAG 
CCAGTCGAGA CTCAACGGAG AAAGCCTGCT GGTACTCCCA CAGGCCAGCA TCGGCACACG 
CAGTTGCACT CATATGGTGC CGACTTTGCC ACCACATTCC AGTCCCTTCC TCCGAGCTCC 
ACCGTCGGCA AAGGTATAAA CCAGGGCGCA ACGGCATCTA GGCAAGGATC GATCTCATCG 
ACAGACATGA CACCACCATC GGACAAGCTC AAAGGCCTTA TCCTTGACTC TTTGCCCGCT 
CATGTCTTCG TAGCCCTTCC ACAAACGGGT GAAATCGTCT GGGTGAACAG TCGGTTCTTG 
ACATACCGCG GGCAAACCTC TAGCGATTTG GCCGCAGACC CTTGGGGGAG CATCCACCCT 
GAAGACAGAG AAGAGTACCT TCGTAAATGG AGTGCGTCTG TCCGTACCGG GGAACCGCTC 
TCAGAGACCG TAAGAATCCG TCGCTTCGAC GGGGCTTATC GGTGGTTCCA AGCGCGGGCT 
GTCGCCTCCA AGGACAAGCG GGGTGTTATC ATGCAGTTCC TGGGTTCATA TATGGATATC 
CATGATAAGC ATGTCGCACA GCTCAGAGCA GCACGGCAGG AAGAGATCGA GGTATCGGAG 
GCAAAGCATC GTCTGCTTGC TAACTTAATC CCCCAGATCA TCTTCACTGC AACTGAAGAC 
GAGGGTATCA CTTTCGCGAA CGAGCAATGG CTTTCTTATA CCGGACAAGG TTTTGATGAT 
GTTCTTGGAC TTGGCTTCAT GGATTTCGTG CACCCAGAGG ATCTGGCTAA ATGCCGGATT 
CCCCTCGAAC GACCCACCTC ACCACATGTC AGGCAGGACA AGACAAAGCA TGACCATTCG 
CCTTCGCCCA GCAAGTTTGG CATGGCCGCT ATGGGCAAAC AGCCTGCTAC CCCTCACGCT 
GAAAATGTGT CTGGGCCCGT ACACCCAGCT CTCACCCGAC ACAGCAGTAG CACCAGCAGT 
TCTGCTAACG ATCTCGCCGG TCCTAACCTG TCCGAGCTTG CCAGGCAGGG AGTCATCAAA 
ATCAGCACCG ACAGCAAGGG CCAGCTCTCA TACACAACAG AGATCCGACT ACGTTCCAAG 
ACCGGCGAGT ATCGCTGGCA TCTCATTCGA TGCGTTGAGA TTGATAATGT CGACTTTGGT 
AATGGCGTCA GCTCTTATTT CGGCTCTGCC ACCGACATCA ACGATCTGAA ACTCCTGGAA 
ACCAAGTTAA AGGAGGCTAT GGAGTCGAAG AGCCGTTTCC TTAGCAACAT GTCGCATGAG 
ATCCGAACCC CTCTTATTGG CATTTCTGGT ATGGTCAGTT TCCTTCAGGA TACCACACTC 
AACGAGGAGC AACGTGATTA CACGAATACC ATTCAGACCA GCGCCAACAG TCTGATCATG 
ATCATCAACG ATATCCTGGA TCTTTCCAAG GTTGATGCAG GCATGATGAA GTTGAACTAC 
GAATGGTTTC ACACGCGCTC TCTCATCGAG GATGTCAACG AGCTGGTGTC CACCATGGCG 
ATCGCCAAGC GTCTCGAGCT CAACTATGTT GTCGAAGAAG ACGTGCCTGC ATGGGTCAAG 
GGTGATAAGG TCCGAATCCG CCAGGTCTTG CTCAACGTCA TCGGCAATGC CATCAAGTTC 
ACTAATGAAG GAGAGGTATT CAGCCGCTGC AAGGTTGTTG TTCCCAACCA TGGCCATTTG 
TACGAGAACG AGATTATGCT TGAGTTCTCC ATCATTGACA CTGGAAGAGG CTTCACCGAA 
GAGGAAGCCG AGTTGATCTT CAAACCCTTC AGCCAGATTG ATGGAAGCAG TACCCGCCAA 
CACGGTGGAA GTGGCCTAGG TTTGGTCATT TCGCGCCAAC TGGTCGAGCT ACACGGCGGT 
AAGATGGACG GTAAGGCGGT GCTAGGAAAG GGATCAACGT TCACCTTTAC CGCAAGGTTT 
GGGCTTCCCA CAGAATCCGA CAACCCTGAC CTGGCTGCAC CACCTGCGCC TACGCCAGGC 
CTTATACCGG AAATGAACAT TCTCCGTCGG TCCTCCATGG CATCCCCTCA CAGTAGGCCA 
TATCTCACTT CTGCACCTTC ATCGGGCTCA GTGGTACCTG TTGAGTCTGA CACTAGATCG 
CCGGCAGCAG TCTCCACCGG TAGCTCGGAA CCGTCTATCC ACTCGTCACG CACCCGAAAC 
ACTGAACGCT CATCCATATC GTCTGTCAAC GTCGGGTCCT TAGCACGATT TAGCGAGGCT 
GCCCGTGCTA GTGGGCACGA TTTGTCCCAG ATGAAGCTTG AAATGCCTCA TGGCAGAGGA 
GGAGGTGGCT CCGACGCGGG CACGACCCCA ACGCCTAGCA ACTCCAGCAA CGAGCGCCTG 
TCGAGGCCTA CCATTAAAGA GGGCGGTGGT TCAGAAGATA GCGATGGGTA TCACACATCC 
CTATTTTCCA TCTTGATTAT CTGCCCGCAA ACGCACAGCC GCGAAGCCAC GACGAAGCAT 
ATCGAGATGA CGCTGCCAAA GAGTAACCCT CACCAGATTA CAGCGGTCGC ATCTGTTCAG 
GAGGCGCGCA AGCTGATCGG TGGTGAGGAC CCCGTCTTCT TCACGCACGT GGTGGTTAAC 
CTTCCGACAC CTGAGCAGAT CATTGAGTTG ATGGATGAAG TCCAGCGCTC AACCATGATT 
GGCCGCGGCG TTACCATCCT CATTCTCTCC GACTCGGTAC AGCGACAGGC GGTTCTGAAG 
CAGGTAGCCG GTACCCAGTA TGAGGACATT TTGTCTGAAA ACCGGGTGAC CTATATCTAC 
AAACCTGTCA AGCCTTCACG GTTCGCGGTG ATCTTTGATC CGGCCAAGGA GCGTGATCTC 
AGCATCGACC GGAACAGATC AACGGCAGAG CAGTTAGTGG AAAACCAAAA GCAGAGTTAC 
CTTGAGGTGG AGAAGCGCAT GGGCAACAAG GGGTACAAAG TCCTCTTAGT TGAGGACAAC 
TTGGTCAACC AAAAGGTGCT GCAAAAGTAT CTTAAGAAGG TCGGCGTGGA CGTGGAGTTG 
GTCGCAGACG GAGTAGAGTG CACTGATGTC GTGTTTTCGC GGCCTCATGA CTACTTTTCG 
TTGATCTTGT GCGATCTCCA CATGCCACGC AAGGACGGAT ATCAAGCCTG CCGTGAAATT 
CGTGAGTGGG AAGCCAAGGG CAACTTTCCT GGAAGGGCGT TGCCCATCAT TGCCCTGTCG 
GCAAACGTCA TGAGTGACGT CCAGGATAAG TGTCTCCAAG CAGGGTTCAA CGACTACGTA 
ACGAAACCTG TCGACTTTAT CAATCTGAGT AATGCTCTTT CGAGGTTCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393513.1
CDS	981..7514
Translation	MRFFSLHRRSHSKEKEKEKEKEKEKRKSITLPLLRPRSTERPRTTESTLTSTTTTTPALTPTPTLTITDTTAATTPTITTAKPVTPPRTTTPPRTVIESPDSHCSNQYAIARSHTGPTDGYALNPPEASAPSEQLEHQDQMTGGANGRGGLGSLQLPTPHLRASNQHTLAPVVDDIRSRDSVRKPSRLSSEVSFNDSPFSEPTSEERDLQQVENGKRTVGESQRGKMDTSEAKRQLSQYRLHLAREMEKREIVAATENRLAIPGPARVSPIQEEASSPSPQLPQSGSEPTTTSTESGNTIRGGVTPGLTARTPSYPFPRMASSPGFPHSGLPFHRPFTTLSPTTFPTTHSSTGAHVSFAGIQGLDHSVSNPSTPASAMTFLPPGCSGTVQGEFATPNLYDLTLMLSAEPGLDAWWHTVVQIMTEVYRAERVTLAVPADPTDIENVPWGQKATYNPHQEDGLSLGYLARGSSFVHSSTDDITDLYQNNDESLKPKGLARPGLPSRHSFSSYEDSKDKKESIERTPVTPLRPPMASRSKTAHPGVGRDRDVSEDDDVAVSLNREALAEHDALEGQQPIPSWEAPITNKREGHGKLLNVLQALDYEADPLIDHNGVLRVLERGRVVALTRSYPYLTQTSSTDSKAADPRVATRSQSPEGPRKKIKKIRTRSESATKLSALLGSATLSTAAGRTSGGHGLEKKATGIASRLDDEYPRPPTPKYEEYEQAPPSPWSQSPAPSPAVRADPSENPFFADAMVDEESFNPGSAPPDYSALQPPEAIGVDNSWTVLHIPLTHVLLSKPQQPGFKLDSTFLEQKMSQRGKSSHTPTIPTPYQEPVAKEKHAPIAILSILSPIIPYPTNLRHSLEHLAPHLATSFSLCRHYTNLETELNGLQKRRPQTVGFGALGPDGRPLADPTAFTMGYLPTDDSISQQSHAGSITSPSEYSGPSRSVIHSPNGTPTWDSSALSYLMGKRSSSASPAPVAAGDSYFTTTPKPGGTGVGQRTKTGSRESTTLERKGSLKIPGGKSQWQDAAGVAPVSQESQGSGESNRRSIDEPSPTNSQQKTAVLKEQREERGGIASSQPVETQRRKPAGTPTGQHRHTQLHSYGADFATTFQSLPPSSTVGKGINQGATASRQGSISSTDMTPPSDKLKGLILDSLPAHVFVALPQTGEIVWVNSRFLTYRGQTSSDLAADPWGSIHPEDREEYLRKWSASVRTGEPLSETVRIRRFDGAYRWFQARAVASKDKRGVIMQFLGSYMDIHDKHVAQLRAARQEEIEVSEAKHRLLANLIPQIIFTATEDEGITFANEQWLSYTGQGFDDVLGLGFMDFVHPEDLAKCRIPLERPTSPHVRQDKTKHDHSPSPSKFGMAAMGKQPATPHAENVSGPVHPALTRHSSSTSSSANDLAGPNLSELARQGVIKISTDSKGQLSYTTEIRLRSKTGEYRWHLIRCVEIDNVDFGNGVSSYFGSATDINDLKLLETKLKEAMESKSRFLSNMSHEIRTPLIGISGMVSFLQDTTLNEEQRDYTNTIQTSANSLIMIINDILDLSKVDAGMMKLNYEWFHTRSLIEDVNELVSTMAIAKRLELNYVVEEDVPAWVKGDKVRIRQVLLNVIGNAIKFTNEGEVFSRCKVVVPNHGHLYENEIMLEFSIIDTGRGFTEEEAELIFKPFSQIDGSSTRQHGGSGLGLVISRQLVELHGGKMDGKAVLGKGSTFTFTARFGLPTESDNPDLAAPPAPTPGLIPEMNILRRSSMASPHSRPYLTSAPSSGSVVPVESDTRSPAAVSTGSSEPSIHSSRTRNTERSSISSVNVGSLARFSEAARASGHDLSQMKLEMPHGRGGGGSDAGTTPTPSNSSNERLSRPTIKEGGGSEDSDGYHTSLFSILIICPQTHSREATTKHIEMTLPKSNPHQITAVASVQEARKLIGGEDPVFFTHVVVNLPTPEQIIELMDEVQRSTMIGRGVTILILSDSVQRQAVLKQVAGTQYEDILSENRVTYIYKPVKPSRFAVIFDPAKERDLSIDRNRSTAEQLVENQKQSYLEVEKRMGNKGYKVLLVEDNLVNQKVLQKYLKKVGVDVELVADGVECTDVVFSRPHDYFSLILCDLHMPRKDGYQACREIREWEAKGNFPGRALPIIALSANVMSDVQDKCLQAGFNDYVTKPVDFINLSNALSRFF

Target ORF information:

RefSeq Version	XM_011395211.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A two-component histidine kinase CHK-1 (nik-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395211.1

ORF Insert Sequence:

ATGCGATTTT TCAGCCTTCA TCGCCGAAGC CACAGTAAAG AGAAAGAGAA AGAGAAGGAG 
AAGGAGAAGG AGAAAAGAAA GAGCATAACC TTGCCTCTTC TGCGCCCTCG CTCCACCGAA 
AGACCAAGAA CAACCGAATC CACGCTCACA TCCACTACCA CTACGACTCC CGCTCTCACT 
CCTACTCCCA CTCTCACAAT CACAGATACT ACGGCGGCCA CGACGCCTAC GATCACGACC 
GCGAAGCCAG TCACGCCCCC CCGCACGACG ACTCCTCCCA GGACTGTCAT CGAGTCACCC 
GACAGTCACT GTTCTAACCA GTACGCCATT GCGCGATCCC ACACCGGTCC TACCGATGGT 
TATGCCCTGA ATCCTCCAGA GGCTTCTGCG CCGTCTGAAC AGCTTGAACA TCAGGACCAA 
ATGACTGGTG GCGCCAACGG GAGAGGAGGG CTAGGGTCGC TTCAACTTCC AACGCCGCAT 
TTACGGGCTT CCAATCAGCA CACTCTTGCA CCCGTTGTGG ATGATATCCG GTCTCGGGAC 
TCGGTGCGCA AGCCCTCCAG GCTTTCTTCC GAAGTTAGCT TCAACGATAG CCCTTTCAGC 
GAACCGACAT CTGAAGAGAG GGACTTGCAG CAGGTAGAGA ATGGAAAGAG AACAGTCGGG 
GAATCGCAGC GGGGTAAGAT GGATACGTCC GAGGCAAAAC GGCAGCTAAG TCAATACCGT 
TTGCACTTGG CCCGCGAGAT GGAAAAAAGA GAGATTGTTG CGGCAACGGA GAACCGGCTG 
GCCATCCCGG GCCCGGCCCG CGTATCTCCT ATACAGGAGG AGGCCAGCAG TCCCTCGCCA 
CAGCTCCCCC AGAGCGGCAG CGAGCCTACC ACGACATCGA CCGAGTCTGG GAATACCATC 
CGTGGCGGTG TCACCCCCGG CTTGACGGCT AGAACTCCCT CATACCCCTT CCCGCGCATG 
GCATCCTCGC CCGGCTTTCC ACACAGTGGC CTGCCATTTC ACCGCCCGTT TACCACGCTC 
TCACCAACTA CATTTCCGAC TACGCATTCG TCTACGGGCG CTCACGTCTC GTTTGCCGGC 
ATTCAGGGTC TTGACCATTC TGTCTCTAAC CCCTCTACTC CTGCCTCAGC AATGACCTTT 
CTGCCCCCAG GGTGCTCCGG TACTGTGCAA GGTGAATTTG CGACACCCAA TCTATACGAC 
CTGACCCTCA TGCTTTCGGC TGAGCCGGGC TTGGATGCAT GGTGGCACAC AGTTGTTCAG 
ATCATGACTG AAGTGTACAG GGCTGAAAGG GTAACCCTGG CTGTGCCTGC CGATCCGACG 
GATATTGAGA ACGTGCCATG GGGCCAAAAG GCAACGTACA ACCCCCACCA GGAGGATGGC 
CTGAGCCTAG GCTATCTTGC TAGAGGCTCG AGCTTTGTCC ACAGCAGTAC CGACGATATC 
ACCGACCTCT ATCAGAACAA CGACGAATCT CTCAAGCCCA AGGGCTTAGC GCGCCCCGGC 
CTGCCGAGCC GACACTCGTT TTCTTCTTAT GAAGACAGCA AGGACAAGAA GGAGTCGATT 
GAACGAACGC CAGTGACACC CCTTCGGCCA CCAATGGCAT CGCGAAGCAA GACTGCTCAC 
CCAGGAGTCG GACGCGATCG AGATGTGAGT GAGGATGATG ATGTTGCTGT GAGCTTGAAT 
CGTGAAGCTT TGGCAGAGCA TGACGCCCTT GAGGGCCAGC AGCCCATCCC AAGCTGGGAA 
GCACCAATAA CCAATAAGAG AGAAGGGCAC GGGAAGCTCT TGAATGTTCT CCAGGCATTG 
GACTACGAAG CTGATCCATT AATCGATCAC AATGGGGTAT TGCGGGTTTT GGAACGTGGT 
CGTGTTGTTG CTCTTACTAG GTCATACCCG TATCTTACAC AAACATCGTC AACCGACAGC 
AAAGCCGCCG ATCCCAGAGT TGCGACGCGC AGCCAGTCGC CAGAGGGGCC ACGGAAAAAG 
ATCAAGAAAA TTCGCACGCG ATCCGAGTCA GCGACTAAAC TCTCTGCGCT TCTGGGCAGT 
GCGACTTTGT CAACCGCCGC AGGCAGGACC TCGGGCGGGC ACGGCTTGGA GAAGAAAGCT 
ACCGGAATTG CCTCTCGCTT GGATGACGAA TACCCAAGAC CCCCGACACC CAAGTATGAG 
GAGTACGAGC AGGCACCACC TTCTCCGTGG TCTCAATCCC CGGCCCCGTC CCCCGCTGTG 
AGGGCTGACC CTAGCGAAAA TCCGTTCTTT GCGGATGCCA TGGTCGATGA GGAGTCGTTT 
AATCCAGGCT CGGCTCCTCC TGACTACTCA GCTCTGCAAC CACCTGAAGC TATTGGGGTT 
GATAACTCGT GGACGGTGTT GCATATCCCT TTAACCCATG TCCTACTGTC GAAACCTCAG 
CAGCCAGGCT TTAAGCTGGA TTCGACGTTT TTGGAGCAAA AGATGTCACA ACGGGGCAAG 
TCAAGTCACA CGCCGACAAT TCCTACTCCC TACCAAGAAC CTGTCGCCAA GGAAAAGCAC 
GCTCCGATTG CGATTCTGTC TATACTCAGC CCCATTATCC CGTATCCCAC CAATCTACGC 
CACTCGTTGG AGCACTTGGC ACCACATCTG GCCACATCTT TTTCTCTGTG TCGTCACTAT 
ACTAACCTCG AAACGGAGCT GAACGGTCTC CAGAAACGAA GGCCACAGAC GGTTGGCTTT 
GGTGCGCTGG GTCCTGACGG ACGTCCGCTG GCTGATCCTA CCGCTTTCAC AATGGGATAC 
CTCCCAACCG ATGACAGTAT CTCTCAGCAG TCGCACGCTG GCAGCATAAC CAGTCCTAGC 
GAGTACTCTG GTCCCTCTCG CAGTGTCATC CACTCTCCGA ATGGGACGCC TACTTGGGAT 
TCCTCGGCTC TGAGTTATCT AATGGGCAAG AGGTCATCCT CTGCCAGCCC AGCTCCTGTC 
GCCGCTGGGG ACAGTTACTT CACCACCACG CCAAAGCCGG GAGGTACTGG TGTGGGACAA 
CGAACCAAGA CAGGATCACG AGAAAGTACA ACCCTTGAAA GGAAGGGTTC CTTGAAGATA 
CCCGGAGGGA AGTCTCAGTG GCAGGATGCA GCTGGTGTCG CTCCCGTGTC GCAGGAGTCT 
CAGGGAAGCG GAGAAAGCAA CCGCCGGAGT ATTGATGAAC CTTCGCCGAC AAACTCACAA 
CAGAAGACTG CCGTTCTCAA AGAGCAGCGG GAAGAAAGAG GCGGCATCGC AAGCAGTCAG 
CCAGTCGAGA CTCAACGGAG AAAGCCTGCT GGTACTCCCA CAGGCCAGCA TCGGCACACG 
CAGTTGCACT CATATGGTGC CGACTTTGCC ACCACATTCC AGTCCCTTCC TCCGAGCTCC 
ACCGTCGGCA AAGGTATAAA CCAGGGCGCA ACGGCATCTA GGCAAGGATC GATCTCATCG 
ACAGACATGA CACCACCATC GGACAAGCTC AAAGGCCTTA TCCTTGACTC TTTGCCCGCT 
CATGTCTTCG TAGCCCTTCC ACAAACGGGT GAAATCGTCT GGGTGAACAG TCGGTTCTTG 
ACATACCGCG GGCAAACCTC TAGCGATTTG GCCGCAGACC CTTGGGGGAG CATCCACCCT 
GAAGACAGAG AAGAGTACCT TCGTAAATGG AGTGCGTCTG TCCGTACCGG GGAACCGCTC 
TCAGAGACCG TAAGAATCCG TCGCTTCGAC GGGGCTTATC GGTGGTTCCA AGCGCGGGCT 
GTCGCCTCCA AGGACAAGCG GGGTGTTATC ATGCAGTTCC TGGGTTCATA TATGGATATC 
CATGATAAGC ATGTCGCACA GCTCAGAGCA GCACGGCAGG AAGAGATCGA GGTATCGGAG 
GCAAAGCATC GTCTGCTTGC TAACTTAATC CCCCAGATCA TCTTCACTGC AACTGAAGAC 
GAGGGTATCA CTTTCGCGAA CGAGCAATGG CTTTCTTATA CCGGACAAGG TTTTGATGAT 
GTTCTTGGAC TTGGCTTCAT GGATTTCGTG CACCCAGAGG ATCTGGCTAA ATGCCGGATT 
CCCCTCGAAC GACCCACCTC ACCACATGTC AGGCAGGACA AGACAAAGCA TGACCATTCG 
CCTTCGCCCA GCAAGTTTGG CATGGCCGCT ATGGGCAAAC AGCCTGCTAC CCCTCACGCT 
GAAAATGTGT CTGGGCCCGT ACACCCAGCT CTCACCCGAC ACAGCAGTAG CACCAGCAGT 
TCTGCTAACG ATCTCGCCGG TCCTAACCTG TCCGAGCTTG CCAGGCAGGG AGTCATCAAA 
ATCAGCACCG ACAGCAAGGG CCAGCTCTCA TACACAACAG AGATCCGACT ACGTTCCAAG 
ACCGGCGAGT ATCGCTGGCA TCTCATTCGA TGCGTTGAGA TTGATAATGT CGACTTTGGT 
AATGGCGTCA GCTCTTATTT CGGCTCTGCC ACCGACATCA ACGATCTGAA ACTCCTGGAA 
ACCAAGTTAA AGGAGGCTAT GGAGTCGAAG AGCCGTTTCC TTAGCAACAT GTCGCATGAG 
ATCCGAACCC CTCTTATTGG CATTTCTGGT ATGGTCAGTT TCCTTCAGGA TACCACACTC 
AACGAGGAGC AACGTGATTA CACGAATACC ATTCAGACCA GCGCCAACAG TCTGATCATG 
ATCATCAACG ATATCCTGGA TCTTTCCAAG GTTGATGCAG GCATGATGAA GTTGAACTAC 
GAATGGTTTC ACACGCGCTC TCTCATCGAG GATGTCAACG AGCTGGTGTC CACCATGGCG 
ATCGCCAAGC GTCTCGAGCT CAACTATGTT GTCGAAGAAG ACGTGCCTGC ATGGGTCAAG 
GGTGATAAGG TCCGAATCCG CCAGGTCTTG CTCAACGTCA TCGGCAATGC CATCAAGTTC 
ACTAATGAAG GAGAGGTATT CAGCCGCTGC AAGGTTGTTG TTCCCAACCA TGGCCATTTG 
TACGAGAACG AGATTATGCT TGAGTTCTCC ATCATTGACA CTGGAAGAGG CTTCACCGAA 
GAGGAAGCCG AGTTGATCTT CAAACCCTTC AGCCAGATTG ATGGAAGCAG TACCCGCCAA 
CACGGTGGAA GTGGCCTAGG TTTGGTCATT TCGCGCCAAC TGGTCGAGCT ACACGGCGGT 
AAGATGGACG GTAAGGCGGT GCTAGGAAAG GGATCAACGT TCACCTTTAC CGCAAGGTTT 
GGGCTTCCCA CAGAATCCGA CAACCCTGAC CTGGCTGCAC CACCTGCGCC TACGCCAGGC 
CTTATACCGG AAATGAACAT TCTCCGTCGG TCCTCCATGG CATCCCCTCA CAGTAGGCCA 
TATCTCACTT CTGCACCTTC ATCGGGCTCA GTGGTACCTG TTGAGTCTGA CACTAGATCG 
CCGGCAGCAG TCTCCACCGG TAGCTCGGAA CCGTCTATCC ACTCGTCACG CACCCGAAAC 
ACTGAACGCT CATCCATATC GTCTGTCAAC GTCGGGTCCT TAGCACGATT TAGCGAGGCT 
GCCCGTGCTA GTGGGCACGA TTTGTCCCAG ATGAAGCTTG AAATGCCTCA TGGCAGAGGA 
GGAGGTGGCT CCGACGCGGG CACGACCCCA ACGCCTAGCA ACTCCAGCAA CGAGCGCCTG 
TCGAGGCCTA CCATTAAAGA GGGCGGTGGT TCAGAAGATA GCGATGGGTA TCACACATCC 
CTATTTTCCA TCTTGATTAT CTGCCCGCAA ACGCACAGCC GCGAAGCCAC GACGAAGCAT 
ATCGAGATGA CGCTGCCAAA GAGTAACCCT CACCAGATTA CAGCGGTCGC ATCTGTTCAG 
GAGGCGCGCA AGCTGATCGG TGGTGAGGAC CCCGTCTTCT TCACGCACGT GGTGGTTAAC 
CTTCCGACAC CTGAGCAGAT CATTGAGTTG ATGGATGAAG TCCAGCGCTC AACCATGATT 
GGCCGCGGCG TTACCATCCT CATTCTCTCC GACTCGGTAC AGCGACAGGC GGTTCTGAAG 
CAGGTAGCCG GTACCCAGTA TGAGGACATT TTGTCTGAAA ACCGGGTGAC CTATATCTAC 
AAACCTGTCA AGCCTTCACG GTTCGCGGTG ATCTTTGATC CGGCCAAGGA GCGTGATCTC 
AGCATCGACC GGAACAGATC AACGGCAGAG CAGTTAGTGG AAAACCAAAA GCAGAGTTAC 
CTTGAGGTGG AGAAGCGCAT GGGCAACAAG GGGTACAAAG TCCTCTTAGT TGAGGACAAC 
TTGGTCAACC AAAAGGTGCT GCAAAAGTAT CTTAAGAAGG TCGGCGTGGA CGTGGAGTTG 
GTCGCAGACG GAGTAGAGTG CACTGATGTC GTGTTTTCGC GGCCTCATGA CTACTTTTCG 
TTGATCTTGT GCGATCTCCA CATGCCACGC AAGGACGGAT ATCAAGCCTG CCGTGAAATT 
CGTGAGTGGG AAGCCAAGGG CAACTTTCCT GGAAGGGCGT TGCCCATCAT TGCCCTGTCG 
GCAAACGTCA TGAGTGACGT CCAGGATAAG TGTCTCCAAG CAGGGTTCAA CGACTACGTA 
ACGAAACCTG TCGACTTTAT CAATCTGAGT AATGCTCTTT CGAGGTTCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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nik-2 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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