bio-1 cDNA ORF clone, Neurospora crassa OR74A

The following bio-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bio-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf05620 XM_951471.3
Latest version!
Neurospora crassa OR74A biotin synthase (bio-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf05620
    Clone ID Related Accession (Same CDS sequence) XM_951471.3
    Accession Version XM_951471.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1320bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product biotin synthase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506). On Feb 23, 2015 this sequence version replaced XM_951471.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGAAACA CCCACCAGAC TCTCAAGAGA GCGGCGAGTC TCTACAGCAC TTCCCAGTTA 
    AGATACAGCG CCTCATCTAC TCTGCGATTT CAATCCCGCC TTTCACATGC TCAATTCCGC
    TCACTCTCGT CGGCTGCAGG CTCCCAGGTG GCAAACATCC CGGCAACAAA CAAGTACCAG
    AAGCTGGACT CGTTCGCTGG CCGGAACAGT CTCCTACAGG CTACCATTGC AGCAGATGCG
    CCCAGGAATG ACTGGACAAA GGAGGAGATC CGAGAAATAT ACCAAACTCC GCTGATGGAA
    CTGGTCTACT CGGCTTCAAC CCTACACCGA CGATTCTTTG ACCCCGCCAG CGTCCAGCTC
    TGCACGCTCA TGAACATCAA GACAGGTGGA TGCTCAGAAG ACTGCTCCTA CTGCGCCCAG
    TCGAGCCGGT ACAAGACGGG TGTAAAGGCA ACAAAGATAT CATCCGTCGA TGAGGTCCTC
    CAGGCTGCAA AAATCGCAAA AGAAAATGGA TCGACTCGGT TCTGCATGGG GGCCGCCTGG
    AGGGACATGC GGGGCCGCAA GACGAACCTC AAGAACATCA AAGCGATGGT GAGCGGGGTG
    AGGGAGCTCG GTATGGAGGC CTGCGTCACT CTAGGAATGA TTGACGAAAG CCAAGCGGCG
    GAACTGCGCG ATGCCGGACT CACTGCATAC AACCACAACG TCGACACCTC CCGGGAGTAC
    TATCCAAACG TCATCACAAC CCGGACCTAC GACGAGCGAC TCAAGACAAT CAACAACGTC
    CGTCAGGCCG GAATCCACGT CTGCTCGGGT GGCATCCTCG GCCTCGGTGA AGATGACACG
    GACCGTGTGA GCTTCCTATA CACCGTTGCA ACGATGGAAA GCCACCCGGA GAGCTTCCCG
    ATCAACGCTC TCGTCCCCAT CAAGGGAACC CCACTTGGAG ACGCCAGCGC CGCAAAATCG
    GAAACCGGGG AGCCGGAATC GACCGTAACC TTCCACGATA CCGTCCGGAT GGTGGCCACT
    GCACGGCTTG TTCTGCCAGG CTCGGTGATT CGGCTTGCGG CAGGGAGAAT CAATCTGACA
    GAGTCCGAGC AGGCTCTTTG CTTTATGGCT GGAGCAAATG CAATCTTTAC AGGAGAAAAG
    ATGCTGACTA CACCGTGCAG TGGATGGGAC GAGGACAAGG CCATGTTTGA CCGGTGGGGT
    CTCCGGCCGA TGCTTCCAGA CGAGCTGCAT GGATCAGAGA AGAGGCAGCA TGCTGGCACC
    GAAGTCGGCC TTCAGCAGGA GCGTGCTGAT GCTGTGAGAA TGGCGTCTTC GTCTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_956564.1
    CDS305..1624
    Translation

    Target ORF information:

    RefSeq Version XM_951471.3
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A biotin synthase (bio-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_951471.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGAAACA CCCACCAGAC TCTCAAGAGA GCGGCGAGTC TCTACAGCAC TTCCCAGTTA 
    AGATACAGCG CCTCATCTAC TCTGCGATTT CAATCCCGCC TTTCACATGC TCAATTCCGC
    TCACTCTCGT CGGCTGCAGG CTCCCAGGTG GCAAACATCC CGGCAACAAA CAAGTACCAG
    AAGCTGGACT CGTTCGCTGG CCGGAACAGT CTCCTACAGG CTACCATTGC AGCAGATGCG
    CCCAGGAATG ACTGGACAAA GGAGGAGATC CGAGAAATAT ACCAAACTCC GCTGATGGAA
    CTGGTCTACT CGGCTTCAAC CCTACACCGA CGATTCTTTG ACCCCGCCAG CGTCCAGCTC
    TGCACGCTCA TGAACATCAA GACAGGTGGA TGCTCAGAAG ACTGCTCCTA CTGCGCCCAG
    TCGAGCCGGT ACAAGACGGG TGTAAAGGCA ACAAAGATAT CATCCGTCGA TGAGGTCCTC
    CAGGCTGCAA AAATCGCAAA AGAAAATGGA TCGACTCGGT TCTGCATGGG GGCCGCCTGG
    AGGGACATGC GGGGCCGCAA GACGAACCTC AAGAACATCA AAGCGATGGT GAGCGGGGTG
    AGGGAGCTCG GTATGGAGGC CTGCGTCACT CTAGGAATGA TTGACGAAAG CCAAGCGGCG
    GAACTGCGCG ATGCCGGACT CACTGCATAC AACCACAACG TCGACACCTC CCGGGAGTAC
    TATCCAAACG TCATCACAAC CCGGACCTAC GACGAGCGAC TCAAGACAAT CAACAACGTC
    CGTCAGGCCG GAATCCACGT CTGCTCGGGT GGCATCCTCG GCCTCGGTGA AGATGACACG
    GACCGTGTGA GCTTCCTATA CACCGTTGCA ACGATGGAAA GCCACCCGGA GAGCTTCCCG
    ATCAACGCTC TCGTCCCCAT CAAGGGAACC CCACTTGGAG ACGCCAGCGC CGCAAAATCG
    GAAACCGGGG AGCCGGAATC GACCGTAACC TTCCACGATA CCGTCCGGAT GGTGGCCACT
    GCACGGCTTG TTCTGCCAGG CTCGGTGATT CGGCTTGCGG CAGGGAGAAT CAATCTGACA
    GAGTCCGAGC AGGCTCTTTG CTTTATGGCT GGAGCAAATG CAATCTTTAC AGGAGAAAAG
    ATGCTGACTA CACCGTGCAG TGGATGGGAC GAGGACAAGG CCATGTTTGA CCGGTGGGGT
    CTCCGGCCGA TGCTTCCAGA CGAGCTGCAT GGATCAGAGA AGAGGCAGCA TGCTGGCACC
    GAAGTCGGCC TTCAGCAGGA GCGTGCTGAT GCTGTGAGAA TGGCGTCTTC GTCTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B