Cers1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cers1
Entrez Gene ID	93898
Full Name	ceramide synthase 1
Synonyms	Lass1,Uog-1,to
General protein information	Preferred Names ceramide synthase 1 Names ceramide synthase 1 LAG1 homolog, ceramide synthase 1 LAG1 longevity assurance homolog 1 longevity assurance gene 1 protein homolog 1 longevity assurance homolog 1 protein UOG-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 B3.3
Summary	This gene encodes a ceramide synthase enzyme, which catalyzes the synthesis of ceramide, the hydrophobic moiety of sphingolipids. The encoded enzyme synthesizes 18-carbon (C18) ceramide in brain neurons. Mice lacking a functional copy of this gene exhibit impaired cerebellar development, locomotion and motor coordination. This protein is transcribed from a bicistronic mRNA, which also encodes growth differentiation factor 1. [provided by RefSeq, Jul 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138647.3	NP_619588.1	ceramide synthase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LASS1	XP_524160.3
Homo sapiens (human)	CERS1	NP_067090.1
Gallus gallus (chicken)	CERS1	NP_001264694.1
Caenorhabditis elegans (roundworm)	lagr-1	NP_493403.1
Danio rerio (zebrafish)	cers1	XP_005161672.1
Bos taurus (cattle)	CERS1	NP_001076983.1
Mus musculus (house mouse)	Cers1	NP_619588.1
Rattus norvegicus (Norway rat)	Cers1	NP_001037695.1
Xenopus tropicalis (tropical clawed frog)	cers1	XP_002940648.1

Gene Ontology
Bibliography

Related articles in PubMed

Ectopic expression of ceramide synthase 2 in neurons suppresses neurodegeneration induced by ceramide synthase 1 deficiency.
Spassieva SD, Ji X, Liu Y, Gable K, Bielawski J, Dunn TM, Bieberich E, Zhao L
Proceedings of the National Academy of Sciences of the United States of America113(21)5928-33(2016 May)

SIRT3 Deacetylates Ceramide Synthases: IMPLICATIONS FOR MITOCHONDRIAL DYSFUNCTION AND BRAIN INJURY.
Novgorodov SA, Riley CL, Keffler JA, Yu J, Kindy MS, Macklin WB, Lombard DB, Gudz TI
The Journal of biological chemistry291(4)1957-73(2016 Jan)

Histological analyses by matrix-assisted laser desorption/ionization-imaging mass spectrometry reveal differential localization of sphingomyelin molecular species regulated by particular ceramide synthase in mouse brains.
Sugimoto M, Shimizu Y, Yoshioka T, Wakabayashi M, Tanaka Y, Higashino K, Numata Y, Sakai S, Kihara A, Igarashi Y, Kuge Y
Biochimica et biophysica acta1851(12)1554-65(2015 Dec)

Morphology-based mammalian stem cell tests reveal potential developmental toxicity of donepezil.
Lau CG, Marikawa Y
Molecular reproduction and development81(11)994-1008(2014 Nov)

Oxidized phospholipids induce ceramide accumulation in RAW 264.7 macrophages: role of ceramide synthases.
Halasiddappa LM, Koefeler H, Futerman AH, Hermetter A
PloS one8(7)e70002(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The strong association of LCB levels with neuronal survival both in vivo and in vitro suggests high-level accumulation of LCBs is a possible underlying cause of the CerS1 deficiency-induced neuronal death.
Title: Ectopic expression of ceramide synthase 2 in neurons suppresses neurodegeneration induced by ceramide synthase 1 deficiency.

CerS1, -2, and -6 are hyperacetylated in the mitochondria of SIRT3-null mice.
Title: SIRT3 Deacetylates Ceramide Synthases: IMPLICATIONS FOR MITOCHONDRIAL DYSFUNCTION AND BRAIN INJURY.

study is the first comparison of spatial distribution between SM molecular species and CerS isoforms, and revealed their distinct association in the brain.
Title: Histological analyses by matrix-assisted laser desorption/ionization-imaging mass spectrometry reveal differential localization of sphingomyelin molecular species regulated by particular ceramide synthase in mouse brains.

Data indicate that oxidized phospholipids (OxPLs)-induced ceramide synthases (CerS1-Cers6) activity in macrophages is responsible for the accumulation of ceramide.
Title: Oxidized phospholipids induce ceramide accumulation in RAW 264.7 macrophages: role of ceramide synthases.

an essential function of CerS1-derived ceramide in the regulation of cerebellar development and neurodevelopmentally regulated behavior.
Title: Ablation of neuronal ceramide synthase 1 in mice decreases ganglioside levels and expression of myelin-associated glycoprotein in oligodendrocytes.

The following Cers1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cers1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19051	NM_138647.3 Latest version!	Mus musculus ceramide synthase 1 (Cers1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cers1 ( NM_138647.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_619588.1 Mus musculus ceramide synthase 1 (Cers1), mRNA.

Price & Availability↑

CloneID	OMu19051
Clone ID Related Accession (Same CDS sequence)	NM_138647.3
Accession Version	NM_138647.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	ceramide synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB665603.1, M62301.1 and BC056973.1. On Jul 15, 2009 this sequence version replaced NM_138647.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056973.1, AK053885.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## bicistronic transcript :: PMID: 2034669 NMD candidate :: PMID: 2034669 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCTG CCGCGGCGAC CCCCAGGCTC GAGGCGCCAG AGCCCATGCC GAGTTATGCG 
CAGATGTTGC AACGAAGCTG GGCCTCGGCG CTGGCGGCGG CTCAGGGCTG CGGGGACTGC 
GGCTGGGGAC TGGCGCGCCG CGGCCTGGCG GAGCACGCGC ACCTGGCTGC ACCCGAGCTG 
CTGCTGGCCG TGCTCTGCGC TCTGGGGTGG ACAGCGTTGC GCTGGGCAGC CACCACACAC 
ATCTTTCGGC CCCTGGCCAA GCGGTGTCGT CTGCAGCCTA GAGATGCTGC CAGGTTACCT 
GAGAGCGCCT GGAAGCTTCT GTTCTACTTG GCCTGTTGGA GCTACTGCGC TTACCTGCTC 
CTGGGCACCA GTTATCCTTT CTTCCATGAC CCGCCCTCTG TCTTCTATGA CTGGAGGTCA 
GGCATGGCAG TGCCCTGGGA CATCGCGGTG GCCTATTTGC TGCAGGGGAG TTTCTACTGC 
CACTCCATCT ATGCCACCGT GTACATGGAC AGCTGGCGTA AGGACTCGGT GGTCATGCTG 
GTGCATCACG TGGTCACCCT GCTCCTCATT GCCTCTTCCT ACGCCTTCCG GTACCACAAC 
GTAGGCCTCC TCGTGTTCTT CCTGCATGAC GTCAGCGATG TGCAGCTGGA GTTCACAAAA 
CTCAACATCT ACTTTAAGGC TAGGGGTGGT GCCTACCATC GCTTGCATGG GCTGGTGGCC 
AACCTGGGCT GCCTCAGCTT CTGTTTCTGC TGGTTCTGGT TCCGCCTCTA CTGGTTCCCG 
CTCAAGGTTC TGTACGCCAC TTGCCACTGC AGCCTGCAGT CTGTGCCTGA CATTCCGTAC 
TACTTCTTCT TCAACATTCT GCTGTTGCTC CTGATGGTCA TGAACATCTA TTGGTTCCTG 
TACATTGTGG CTTTCGCAGC CAAGGTGCTG ACTGGTCAGA TGCGTGAACT GGAAGACTTG 
AGGGAGTACG ACACTCTGGA AGCTCAGACA GCCAAGCCCT GCAAAGCCGA GAAGCCACTG 
AGGAATGGCC TGGTGAAGGA CAAGCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619588.1
CDS	86..1138
Translation	MAAAAATPRLEAPEPMPSYAQMLQRSWASALAAAQGCGDCGWGLARRGLAEHAHLAAPELLLAVLCALGWTALRWAATTHIFRPLAKRCRLQPRDAARLPESAWKLLFYLACWSYCAYLLLGTSYPFFHDPPSVFYDWRSGMAVPWDIAVAYLLQGSFYCHSIYATVYMDSWRKDSVVMLVHHVVTLLLIASSYAFRYHNVGLLVFFLHDVSDVQLEFTKLNIYFKARGGAYHRLHGLVANLGCLSFCFCWFWFRLYWFPLKVLYATCHCSLQSVPDIPYYFFFNILLLLLMVMNIYWFLYIVAFAAKVLTGQMRELEDLREYDTLEAQTAKPCKAEKPLRNGLVKDKLF

Target ORF information:

RefSeq Version	NM_138647.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ceramide synthase 1 (Cers1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138647.3

ORF Insert Sequence:

ATGGCTGCTG CCGCGGCGAC CCCCAGGCTC GAGGCGCCAG AGCCCATGCC GAGTTATGCG 
CAGATGTTGC AACGAAGCTG GGCCTCGGCG CTGGCGGCGG CTCAGGGCTG CGGGGACTGC 
GGCTGGGGAC TGGCGCGCCG CGGCCTGGCG GAGCACGCGC ACCTGGCTGC ACCCGAGCTG 
CTGCTGGCCG TGCTCTGCGC TCTGGGGTGG ACAGCGTTGC GCTGGGCAGC CACCACACAC 
ATCTTTCGGC CCCTGGCCAA GCGGTGTCGT CTGCAGCCTA GAGATGCTGC CAGGTTACCT 
GAGAGCGCCT GGAAGCTTCT GTTCTACTTG GCCTGTTGGA GCTACTGCGC TTACCTGCTC 
CTGGGCACCA GTTATCCTTT CTTCCATGAC CCGCCCTCTG TCTTCTATGA CTGGAGGTCA 
GGCATGGCAG TGCCCTGGGA CATCGCGGTG GCCTATTTGC TGCAGGGGAG TTTCTACTGC 
CACTCCATCT ATGCCACCGT GTACATGGAC AGCTGGCGTA AGGACTCGGT GGTCATGCTG 
GTGCATCACG TGGTCACCCT GCTCCTCATT GCCTCTTCCT ACGCCTTCCG GTACCACAAC 
GTAGGCCTCC TCGTGTTCTT CCTGCATGAC GTCAGCGATG TGCAGCTGGA GTTCACAAAA 
CTCAACATCT ACTTTAAGGC TAGGGGTGGT GCCTACCATC GCTTGCATGG GCTGGTGGCC 
AACCTGGGCT GCCTCAGCTT CTGTTTCTGC TGGTTCTGGT TCCGCCTCTA CTGGTTCCCG 
CTCAAGGTTC TGTACGCCAC TTGCCACTGC AGCCTGCAGT CTGTGCCTGA CATTCCGTAC 
TACTTCTTCT TCAACATTCT GCTGTTGCTC CTGATGGTCA TGAACATCTA TTGGTTCCTG 
TACATTGTGG CTTTCGCAGC CAAGGTGCTG ACTGGTCAGA TGCGTGAACT GGAAGACTTG 
AGGGAGTACG ACACTCTGGA AGCTCAGACA GCCAAGCCCT GCAAAGCCGA GAAGCCACTG 
AGGAATGGCC TGGTGAAGGA CAAGCTCTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ectopic expression of ceramide synthase 2 in neurons suppresses neurodegeneration induced by ceramide synthase 1 deficiency.
Proceedings of the National Academy of Sciences of the United States of America113(21)5928-33(2016 May)
Spassieva SD,Ji X,Liu Y,Gable K,Bielawski J,Dunn TM,Bieberich E,Zhao L

SIRT3 Deacetylates Ceramide Synthases: IMPLICATIONS FOR MITOCHONDRIAL DYSFUNCTION AND BRAIN INJURY.
The Journal of biological chemistry291(4)1957-73(2016 Jan)
Novgorodov SA,Riley CL,Keffler JA,Yu J,Kindy MS,Macklin WB,Lombard DB,Gudz TI

Histological analyses by matrix-assisted laser desorption/ionization-imaging mass spectrometry reveal differential localization of sphingomyelin molecular species regulated by particular ceramide synthase in mouse brains.
Biochimica et biophysica acta1851(12)1554-65(2015 Dec)
Sugimoto M,Shimizu Y,Yoshioka T,Wakabayashi M,Tanaka Y,Higashino K,Numata Y,Sakai S,Kihara A,Igarashi Y,Kuge Y

Morphology-based mammalian stem cell tests reveal potential developmental toxicity of donepezil.
Molecular reproduction and development81(11)994-1008(2014 Nov)
Lau CG,Marikawa Y

Oxidized phospholipids induce ceramide accumulation in RAW 264.7 macrophages: role of ceramide synthases.
PloS one8(7)e70002(2013)
Halasiddappa LM,Koefeler H,Futerman AH,Hermetter A

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Cers1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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