SNURF cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	SNURF
Entrez Gene ID	84704
Full Name	SNRPN upstream reading frame
Synonyms	2410045I01Rik
General protein information	Preferred Names SNRPN upstream reading frame Names SNRPN upstream reading frame protein small nuclear ribonucleoprotein N
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B5
Summary	This gene is located within the mouse orthologous Prader-Willi Syndrome critical region and is imprinted and expressed from the paternal allele. This transcipt is thought to be bicistronic and can encode the small nuclear ribonucleoprotein polypeptide N (Snrpn) from a downstream open reading frame. The small protein represented by this gene is encoded by an evolutionarily-conserved upstream open reading frame and is localized to the nucleus. [provided by RefSeq, Mar 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_033174.4	NP_149409.1	SNRPN upstream reading frame protein
NM_033174.3	NP_149409.1	SNRPN upstream reading frame protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC100612044	XP_003317775.1
Bos taurus (cattle)	SNURF	NP_776888.1
Mus musculus (house mouse)	Snurf	NP_149409.1
Rattus norvegicus (Norway rat)	Snurf	NP_001257641.1
Homo sapiens (human)	SNURF	NP_005669.2

Gene Ontology
Bibliography

Related articles in PubMed

Global deficits in development, function, and gene expression in the endocrine pancreas in a deletion mouse model of Prader-Willi syndrome.
Stefan M, Simmons RA, Bertera S, Trucco M, Esni F, Drain P, Nicholls RD
American journal of physiology. Endocrinology and metabolism300(5)E909-22(2011 May)

Developmentally dynamic changes of DNA methylation in the mouse Snurf/Snrpn gene.
Miyazaki K, Mapendano CK, Fuchigami T, Kondo S, Ohta T, Kinoshita A, Tsukamoto K, Yoshiura K, Niikawa N, Kishino T
Gene432(1-2)97-101(2009 Mar)

Loss of methylation imprint of Snrpn in postovulatory aging mouse oocyte.
Liang XW, Zhu JQ, Miao YL, Liu JH, Wei L, Lu SS, Hou Y, Schatten H, Lu KH, Sun QY
Biochemical and biophysical research communications371(1)16-21(2008 Jun)

Disruption of parental-specific expression of imprinted genes in uniparental fetuses.
Ogawa H, Wu Q, Komiyama J, Obata Y, Kono T
FEBS letters580(22)5377-84(2006 Oct)

Expression of the Snurf-Snrpn IC transcript in the oocyte and its putative role in the imprinting establishment of the mouse 7C imprinting domain.
Mapendano CK, Kishino T, Miyazaki K, Kondo S, Yoshiura K, Hishikawa Y, Koji T, Niikawa N, Ohta T
Journal of human genetics51(3)236-43(2006)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the ATG-to-AAG mutation causes a 15-fold or more increase in translation of the downstream ORF in two fusion constructs, and it is likely that similar translational control affects the normal Snurf-Snrpn transcript as well
Title: Evidence for translational regulation of the imprinted Snurf-Snrpn locus in mice.

The following SNURF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SNURF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13647	NM_033174.4 Latest version!	Mus musculus SNRPN upstream reading frame (Snurf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu13647	NM_033174.3	Mus musculus SNRPN upstream reading frame (Snurf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SNURF ( NM_033174.4 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_149409.1 Mus musculus SNRPN upstream reading frame (Snurf), mRNA.

Price & Availability↑

CloneID	OMu13647
Clone ID Related Accession (Same CDS sequence)	NM_033174.4 , NM_033174.3
Accession Version	NM_033174.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	SNRPN upstream reading frame protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC167813.4, BC132082.1, CF751578.1, BY648312.1 and AC172750.1. On Mar 23, 2017 this sequence version replaced NM_033174.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.105967.1, SRR1660817.202313.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164141 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## bicistronic transcript :: PMID: 10318933 imprinted gene :: PMID: 11431693 NMD candidate :: PMID: 10318933 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGAG GAAGGGATCG CTTACACTTG AGAAGAACTA CTGAACAGCA CGTGCCCGAG 
GTCGAGGTCC AGGTCAAACG TCGAAGGACA GCCTCACTGA GCAACCAAGA GTGTCACTTG 
TACCCACGAC GTTCTCAGCA ACAGCAAGTT CCTGTGGTGG ATTTCCAGGC AGAACTAAGA 
CAGGCATTCT TAGCTGAGAC ACCAAGAGGT GGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149409.1
CDS	151..366
Translation	MERGRDRLHLRRTTEQHVPEVEVQVKRRRTASLSNQECHLYPRRSQQQQVPVVDFQAELRQAFLAETPRGG

Target ORF information:

RefSeq Version	NM_033174.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SNRPN upstream reading frame (Snurf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033174.4

ORF Insert Sequence:

ATGGAGCGAG GAAGGGATCG CTTACACTTG AGAAGAACTA CTGAACAGCA CGTGCCCGAG 
GTCGAGGTCC AGGTCAAACG TCGAAGGACA GCCTCACTGA GCAACCAAGA GTGTCACTTG 
TACCCACGAC GTTCTCAGCA ACAGCAAGTT CCTGTGGTGG ATTTCCAGGC AGAACTAAGA 
CAGGCATTCT TAGCTGAGAC ACCAAGAGGT GGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNURF ( NM_033174.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_149409.1 Mus musculus SNRPN upstream reading frame (Snurf), mRNA.

Price & Availability↑

CloneID	OMu13647
Clone ID Related Accession (Same CDS sequence)	NM_033174.4 , NM_033174.3
Accession Version	NM_033174.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	SNRPN upstream reading frame protein
Comment

ATGGAGCGAG GAAGGGATCG CTTACACTTG AGAAGAACTA CTGAACAGCA CGTGCCCGAG 
GTCGAGGTCC AGGTCAAACG TCGAAGGACA GCCTCACTGA GCAACCAAGA GTGTCACTTG 
TACCCACGAC GTTCTCAGCA ACAGCAAGTT CCTGTGGTGG ATTTCCAGGC AGAACTAAGA 
CAGGCATTCT TAGCTGAGAC ACCAAGAGGT GGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149409.1
Translation	MERGRDRLHLRRTTEQHVPEVEVQVKRRRTASLSNQECHLYPRRSQQQQVPVVDFQAELRQAFLAETPRGG

Target ORF information:

RefSeq Version	NM_033174.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus SNRPN upstream reading frame (Snurf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033174.3

ORF Insert Sequence:

ATGGAGCGAG GAAGGGATCG CTTACACTTG AGAAGAACTA CTGAACAGCA CGTGCCCGAG 
GTCGAGGTCC AGGTCAAACG TCGAAGGACA GCCTCACTGA GCAACCAAGA GTGTCACTTG 
TACCCACGAC GTTCTCAGCA ACAGCAAGTT CCTGTGGTGG ATTTCCAGGC AGAACTAAGA 
CAGGCATTCT TAGCTGAGAC ACCAAGAGGT GGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Global deficits in development, function, and gene expression in the endocrine pancreas in a deletion mouse model of Prader-Willi syndrome.
American journal of physiology. Endocrinology and metabolism300(5)E909-22(2011 May)
Stefan M,Simmons RA,Bertera S,Trucco M,Esni F,Drain P,Nicholls RD

Developmentally dynamic changes of DNA methylation in the mouse Snurf/Snrpn gene.
Gene432(1-2)97-101(2009 Mar)
Miyazaki K,Mapendano CK,Fuchigami T,Kondo S,Ohta T,Kinoshita A,Tsukamoto K,Yoshiura K,Niikawa N,Kishino T

Loss of methylation imprint of Snrpn in postovulatory aging mouse oocyte.
Biochemical and biophysical research communications371(1)16-21(2008 Jun)
Liang XW,Zhu JQ,Miao YL,Liu JH,Wei L,Lu SS,Hou Y,Schatten H,Lu KH,Sun QY

Disruption of parental-specific expression of imprinted genes in uniparental fetuses.
FEBS letters580(22)5377-84(2006 Oct)
Ogawa H,Wu Q,Komiyama J,Obata Y,Kono T

Expression of the Snurf-Snrpn IC transcript in the oocyte and its putative role in the imprinting establishment of the mouse 7C imprinting domain.
Journal of human genetics51(3)236-43(2006)
Mapendano CK,Kishino T,Miyazaki K,Kondo S,Yoshiura K,Hishikawa Y,Koji T,Niikawa N,Ohta T

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SNURF cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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