Pramel1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pramel1
Entrez Gene ID	83491
Full Name	preferentially expressed antigen in melanoma-like 1
General protein information	Preferred Names preferentially expressed antigen in melanoma-like 1 Names preferentially expressed antigen in melanoma-like protein 1 preferentially expressed antigen in melanoma-like 1 prame-like 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_031377.2	NP_113554.1	preferentially expressed antigen in melanoma-like protein 1
XM_006539316.2	XP_006539379.1	preferentially expressed antigen in melanoma-like 1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pramel1	NP_113554.1
Rattus norvegicus (Norway rat)	Pramel1	NP_001103087.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential expression of PRAMEL1, a cancer/testis antigen, during spermatogenesis in the mouse.
Mistry BV, Zhao Y, Chang TC, Yasue H, Chiba M, Oatley J, Diaz F, Liu WS
PloS one8(4)e60611(2013)

Identification of a new expanding family of genes characterized by atypical LRR domains. Localization of a cluster preferentially expressed in oocyte.
Dad? S, Callebaut I, Mermillod P, Monget P
FEBS letters555(3)533-8(2003 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pramel1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pramel1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16305	NM_031377.2 Latest version!	Mus musculus preferentially expressed antigen in melanoma-like 1 (Pramel1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00
OMu34516	XM_006539316.2 Latest version!	Mus musculus preferentially expressed antigen in melanoma-like 1 (Pramel1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pramel1 ( NM_031377.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_113554.1 Mus musculus preferentially expressed antigen in melanoma-like 1 (Pramel1), mRNA.

Price & Availability↑

CloneID	OMu16305
Clone ID Related Accession (Same CDS sequence)	NM_031377.2
Accession Version	NM_031377.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	preferentially expressed antigen in melanoma-like protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL611930.24. On Jul 14, 2009 this sequence version replaced NM_031377.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC132399.1, AF285578.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTGCA AGACTCCACC CACACTCCAG GAACTGGCAG AGAACAGCCT CCTGAAGAAC 
CAGGACTTGG CTATCTCTGC TCTGGATGAC ATACCCTCAC TTTTCTTCCC ATCACTGTTC 
AAAAAGGCCT GCAGAAATAG ATATGTTGGG ATCATAAAGG CGATGGTGCA GGCGTGGCCC 
TTCCCCTGTC TTCCTCTGGG GGCCATGATC AGTAGGAAGA CTGCCTACAG GAGAATCTTA 
GAGATTATCC TGTATGGGCT TGATGCCTTG CTTTCCCAGA AAGTTCCCCA CAGCAGGTGC 
AAGCTGCAAG TGCTGGATTT ACGGGTTATG CCTTTGAAGC TGTGGAACAG GTTGCCTGTG 
TTTGGGACTG CTGGCTGCAG TGAGAATCCA GCAGTGGTGG GCCATTCGGG AACAGAGGTG 
AAACAGCCAG TGAAGGTGCT GGTAGACTTG GTCCTCAAGG AAAGCCCACT AGATTCCACA 
GAGTCCTTCC TCGTTCAGTG GGTGGATAAC AGGAATGGTT TGGTGAGTTT GTGCTGTTGC 
AAGCTGCAGA TCTGGGCTAT GTCCATGTAT TACCACAGAA AACTTTTGGA GATTTTGGAT 
CTGGACTCTG TCCAGGAGCT GCGTATGTAC TGCATCAGTA ATCCTGTCTG CCTGCTTAAC 
TTCGCCCCTT ACTTGGGTCG CATGAGGAAC CTGCGCTGCC TCATCCTCTC TCACCTCTGG 
CAGACCTTCT CGATGACCCC GGTGGAGAAG CAGCAGGTTA TTACCCAGTT CACGTCTCAG 
TTCCTCAAAC TGAAATGCCT CCAGATCCTG CATCTGGATA CTGTCTTCTT CCTAGAGGGT 
CATCTGGATG AGCTATTCTG GTGGCTGAAG ACACCCTTAG AGACCCTGTC TGTGATTGAT 
TGTAATCTCT CAAAATCAGA CTGGTTCCAT ATATCTGAGT TCCAGTGCAC AAGTCAGCTA 
AAACACCTGA ATTTGAAATG GGTCAAACTG ACCCATTTGA GCCCAGAGCC CCTTCGAGTT 
CTGTTACTAA AATCTGCATC TACCCTAACA TCCCTGGATT TGGAGGGCTG TCAAATGATG 
GACTCTCAAC TCAGTGCCAT CCTACCTGCT CTGAGATGCT GTACACAGCT CACCAAGTTT 
AATTTCCATG GGAACTATAT CTCCATGCCT ATCCTGAGGG AGCTGGCATA TAACGTTGTC 
AAGCAGAAAT CCCAACAGTC AAAGATACGC TTTATCCCAA GCTGTAGTCA TCACAGTGGT 
CTGGAGTTTG AGGCCATTTC TCAGCCTCAT ATTGTGTTTG TAGATGTGGA TCGGACTACT 
GGGGAGCAAG AACAAGTCTT GTTTTATGCT ATTTGTTCTG GAGAATATGT GTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113554.1
CDS	129..1505
Translation	MSCKTPPTLQELAENSLLKNQDLAISALDDIPSLFFPSLFKKACRNRYVGIIKAMVQAWPFPCLPLGAMISRKTAYRRILEIILYGLDALLSQKVPHSRCKLQVLDLRVMPLKLWNRLPVFGTAGCSENPAVVGHSGTEVKQPVKVLVDLVLKESPLDSTESFLVQWVDNRNGLVSLCCCKLQIWAMSMYYHRKLLEILDLDSVQELRMYCISNPVCLLNFAPYLGRMRNLRCLILSHLWQTFSMTPVEKQQVITQFTSQFLKLKCLQILHLDTVFFLEGHLDELFWWLKTPLETLSVIDCNLSKSDWFHISEFQCTSQLKHLNLKWVKLTHLSPEPLRVLLLKSASTLTSLDLEGCQMMDSQLSAILPALRCCTQLTKFNFHGNYISMPILRELAYNVVKQKSQQSKIRFIPSCSHHSGLEFEAISQPHIVFVDVDRTTGEQEQVLFYAICSGEYVL

Target ORF information:

RefSeq Version	NM_031377.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus preferentially expressed antigen in melanoma-like 1 (Pramel1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031377.2

ORF Insert Sequence:

ATGAGCTGCA AGACTCCACC CACACTCCAG GAACTGGCAG AGAACAGCCT CCTGAAGAAC 
CAGGACTTGG CTATCTCTGC TCTGGATGAC ATACCCTCAC TTTTCTTCCC ATCACTGTTC 
AAAAAGGCCT GCAGAAATAG ATATGTTGGG ATCATAAAGG CGATGGTGCA GGCGTGGCCC 
TTCCCCTGTC TTCCTCTGGG GGCCATGATC AGTAGGAAGA CTGCCTACAG GAGAATCTTA 
GAGATTATCC TGTATGGGCT TGATGCCTTG CTTTCCCAGA AAGTTCCCCA CAGCAGGTGC 
AAGCTGCAAG TGCTGGATTT ACGGGTTATG CCTTTGAAGC TGTGGAACAG GTTGCCTGTG 
TTTGGGACTG CTGGCTGCAG TGAGAATCCA GCAGTGGTGG GCCATTCGGG AACAGAGGTG 
AAACAGCCAG TGAAGGTGCT GGTAGACTTG GTCCTCAAGG AAAGCCCACT AGATTCCACA 
GAGTCCTTCC TCGTTCAGTG GGTGGATAAC AGGAATGGTT TGGTGAGTTT GTGCTGTTGC 
AAGCTGCAGA TCTGGGCTAT GTCCATGTAT TACCACAGAA AACTTTTGGA GATTTTGGAT 
CTGGACTCTG TCCAGGAGCT GCGTATGTAC TGCATCAGTA ATCCTGTCTG CCTGCTTAAC 
TTCGCCCCTT ACTTGGGTCG CATGAGGAAC CTGCGCTGCC TCATCCTCTC TCACCTCTGG 
CAGACCTTCT CGATGACCCC GGTGGAGAAG CAGCAGGTTA TTACCCAGTT CACGTCTCAG 
TTCCTCAAAC TGAAATGCCT CCAGATCCTG CATCTGGATA CTGTCTTCTT CCTAGAGGGT 
CATCTGGATG AGCTATTCTG GTGGCTGAAG ACACCCTTAG AGACCCTGTC TGTGATTGAT 
TGTAATCTCT CAAAATCAGA CTGGTTCCAT ATATCTGAGT TCCAGTGCAC AAGTCAGCTA 
AAACACCTGA ATTTGAAATG GGTCAAACTG ACCCATTTGA GCCCAGAGCC CCTTCGAGTT 
CTGTTACTAA AATCTGCATC TACCCTAACA TCCCTGGATT TGGAGGGCTG TCAAATGATG 
GACTCTCAAC TCAGTGCCAT CCTACCTGCT CTGAGATGCT GTACACAGCT CACCAAGTTT 
AATTTCCATG GGAACTATAT CTCCATGCCT ATCCTGAGGG AGCTGGCATA TAACGTTGTC 
AAGCAGAAAT CCCAACAGTC AAAGATACGC TTTATCCCAA GCTGTAGTCA TCACAGTGGT 
CTGGAGTTTG AGGCCATTTC TCAGCCTCAT ATTGTGTTTG TAGATGTGGA TCGGACTACT 
GGGGAGCAAG AACAAGTCTT GTTTTATGCT ATTTGTTCTG GAGAATATGT GTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pramel1 ( XM_006539316.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539379.1 Mus musculus preferentially expressed antigen in melanoma-like 1 (Pramel1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34516
Clone ID Related Accession (Same CDS sequence)	XM_006539316.2
Accession Version	XM_006539316.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	preferentially expressed antigen in melanoma-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006539316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGGCG GGGCCCGAGC TTATTTCAGC GGCGTGGGAG CAGAACGTTG TGTTAAAGCT 
TCGGCGGCCA CGAGAGCGAG CGCTTCCTGT CAGGTCTCTG TGAGTTTGAG GCCAGCGGGG 
GAGACAGAGG GACCGGAGGG CTCCGGGGCG GCTCGGAGAC ATCTTGCTTC TGTCCATCTC 
TGCAGGTGCA AGCTGCAAGT GCTGGATTTA CGGGTTATGC CTTTGAAGCT GTGGAACAGG 
TTGCCTGTGT TTGGGACTGC TGGCTGCAGT GAGAATCCAG CAGTGGTGGG CCATTCGGGA 
ACAGAGGTGA AACAGCCAGT GAAGGTGCTG GTAGACTTGG TCCTCAAGGA AAGCCCACTA 
GATTCCACAG AGTCCTTCCT CGTTCAGTGG GTGGATAACA GGAATGGTTT GGTGAGTTTG 
TGCTGTTGCA AGCTGCAGAT CTGGGCTATG TCCATGTATT ACCACAGAAA ACTTTTGGAG 
ATTTTGGATC TGGACTCTGT CCAGGAGCTG CGTATGTACT GCATCAGTAA TCCTGTCTGC 
CTGCTTAACT TCGCCCCTTA CTTGGGTCGC ATGAGGAACC TGCGCTGCCT CATCCTCTCT 
CACCTCTGGC AGACCTTCTC GATGACCCCG GTGGAGAAGC AGCAGGTTAT TACCCAGTTC 
ACGTCTCAGT TCCTCAAACT GAAATGCCTC CAGATCCTGC ATCTGGATAC TGTCTTCTTC 
CTAGAGGGTC ATCTGGATGA GCTATTCTGG TGGCTGAAGA CACCCTTAGA GACCCTGTCT 
GTGATTGATT GTAATCTCTC AAAATCAGAC TGGTTCCATA TATCTGAGTT CCAGTGCACA 
AGTCAGCTAA AACACCTGAA TTTGAAATGG GTCAAACTGA CCCATTTGAG CCCAGAGCCC 
CTTCGAGTTC TGTTACTAAA ATCTGCATCT ACCCTAACAT CCCTGGATTT GGAGGGCTGT 
CAAATGATGG ACTCTCAACT CAGTGCCATC CTACCTGCTC TGAGATGCTG TACACAGCTC 
ACCAAGTTTA ATTTCCATGG GAACTATATC TCCATGCCTA TCCTGAGGGA GCTGGCATAT 
AACGTTGTCA AGCAGAAATC CCAACAGTCA AAGATACGCT TTATCCCAAG CTGTAGTCAT 
CACAGTGGTC TGGAGTTTGA GGCCATTTCT CAGCCTCATA TTGTGTTTGT AGATGTGGAT 
CGGACTACTG GGGAGCAAGA ACAAGTCTTG TTTTATGCTA TTTGTTCTGG AGAATATGTG 
TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539379.1
CDS	4..1269
Translation	MGGGARAYFSGVGAERCVKASAATRASASCQVSVSLRPAGETEGPEGSGAARRHLASVHLCRCKLQVLDLRVMPLKLWNRLPVFGTAGCSENPAVVGHSGTEVKQPVKVLVDLVLKESPLDSTESFLVQWVDNRNGLVSLCCCKLQIWAMSMYYHRKLLEILDLDSVQELRMYCISNPVCLLNFAPYLGRMRNLRCLILSHLWQTFSMTPVEKQQVITQFTSQFLKLKCLQILHLDTVFFLEGHLDELFWWLKTPLETLSVIDCNLSKSDWFHISEFQCTSQLKHLNLKWVKLTHLSPEPLRVLLLKSASTLTSLDLEGCQMMDSQLSAILPALRCCTQLTKFNFHGNYISMPILRELAYNVVKQKSQQSKIRFIPSCSHHSGLEFEAISQPHIVFVDVDRTTGEQEQVLFYAICSGEYVL

Target ORF information:

RefSeq Version	XM_006539316.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus preferentially expressed antigen in melanoma-like 1 (Pramel1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539316.2

ORF Insert Sequence:

ATGGGGGGCG GGGCCCGAGC TTATTTCAGC GGCGTGGGAG CAGAACGTTG TGTTAAAGCT 
TCGGCGGCCA CGAGAGCGAG CGCTTCCTGT CAGGTCTCTG TGAGTTTGAG GCCAGCGGGG 
GAGACAGAGG GACCGGAGGG CTCCGGGGCG GCTCGGAGAC ATCTTGCTTC TGTCCATCTC 
TGCAGGTGCA AGCTGCAAGT GCTGGATTTA CGGGTTATGC CTTTGAAGCT GTGGAACAGG 
TTGCCTGTGT TTGGGACTGC TGGCTGCAGT GAGAATCCAG CAGTGGTGGG CCATTCGGGA 
ACAGAGGTGA AACAGCCAGT GAAGGTGCTG GTAGACTTGG TCCTCAAGGA AAGCCCACTA 
GATTCCACAG AGTCCTTCCT CGTTCAGTGG GTGGATAACA GGAATGGTTT GGTGAGTTTG 
TGCTGTTGCA AGCTGCAGAT CTGGGCTATG TCCATGTATT ACCACAGAAA ACTTTTGGAG 
ATTTTGGATC TGGACTCTGT CCAGGAGCTG CGTATGTACT GCATCAGTAA TCCTGTCTGC 
CTGCTTAACT TCGCCCCTTA CTTGGGTCGC ATGAGGAACC TGCGCTGCCT CATCCTCTCT 
CACCTCTGGC AGACCTTCTC GATGACCCCG GTGGAGAAGC AGCAGGTTAT TACCCAGTTC 
ACGTCTCAGT TCCTCAAACT GAAATGCCTC CAGATCCTGC ATCTGGATAC TGTCTTCTTC 
CTAGAGGGTC ATCTGGATGA GCTATTCTGG TGGCTGAAGA CACCCTTAGA GACCCTGTCT 
GTGATTGATT GTAATCTCTC AAAATCAGAC TGGTTCCATA TATCTGAGTT CCAGTGCACA 
AGTCAGCTAA AACACCTGAA TTTGAAATGG GTCAAACTGA CCCATTTGAG CCCAGAGCCC 
CTTCGAGTTC TGTTACTAAA ATCTGCATCT ACCCTAACAT CCCTGGATTT GGAGGGCTGT 
CAAATGATGG ACTCTCAACT CAGTGCCATC CTACCTGCTC TGAGATGCTG TACACAGCTC 
ACCAAGTTTA ATTTCCATGG GAACTATATC TCCATGCCTA TCCTGAGGGA GCTGGCATAT 
AACGTTGTCA AGCAGAAATC CCAACAGTCA AAGATACGCT TTATCCCAAG CTGTAGTCAT 
CACAGTGGTC TGGAGTTTGA GGCCATTTCT CAGCCTCATA TTGTGTTTGT AGATGTGGAT 
CGGACTACTG GGGAGCAAGA ACAAGTCTTG TTTTATGCTA TTTGTTCTGG AGAATATGTG 
TTATGA