Slc35d3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc35d3
Entrez Gene ID	76157
Full Name	solute carrier family 35, member D3
Synonyms	6230421J19Rik,Frcl1
General protein information	Preferred Names solute carrier family 35, member D3 Names solute carrier family 35 member D3 frc, fringe-like 1 fringe connection-like protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006512916.3	XP_006512979.1	solute carrier family 35 member D3 isoform X1
XM_006512916.4	XP_006512979.1	solute carrier family 35 member D3 isoform X1
NM_029529.3	NP_083805.1	solute carrier family 35 member D3

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SLC35D3	XP_426171.2
Arabidopsis thaliana (thale cress)	AT4G31600	NP_567879.1
Canis lupus familiaris (dog)	SLC35D3	XP_003638819.1
Mus musculus (house mouse)	Slc35d3	NP_083805.1
Oryza sativa (rice)	Os06g0188100	NP_001057020.1
Xenopus tropicalis (tropical clawed frog)	slc35d3	XP_002936313.2
Pan troglodytes (chimpanzee)	SLC35D3	XP_518764.2
	SLC35D3	XP_001097534.1
Bos taurus (cattle)	SLC35D3	NP_001179261.1
Danio rerio (zebrafish)	LOC565603	XP_693964.1
Rattus norvegicus (Norway rat)	Slc35d3	NP_001100992.1
Homo sapiens (human)	SLC35D3	NP_001008783.1

Gene Ontology
Bibliography

Related articles in PubMed

SLC35D3 increases autophagic activity in midbrain dopaminergic neurons by enhancing BECN1-ATG14-PIK3C3 complex formation.
Wei ZB, Yuan YF, Jaouen F, Ma MS, Hao CJ, Zhang Z, Chen Q, Yuan Z, Yu L, Beurrier C, Li W
Autophagy12(7)1168-79(2016 Jul)

Mutation of SLC35D3 causes metabolic syndrome by impairing dopamine signaling in striatal D1 neurons.
Zhang Z, Hao CJ, Li CG, Zang DJ, Zhao J, Li XN, Wei AH, Wei ZB, Yang L, He X, Zhen XC, Gao X, Speakman JR, Li W
PLoS genetics10(2)e1004124(2014 Feb)

Dual role for Islet-1 in promoting striatonigral and repressing striatopallidal genetic programs to specify striatonigral cell identity.
Lu KM, Evans SM, Hirano S, Liu FC
Proceedings of the National Academy of Sciences of the United States of America111(1)E168-77(2014 Jan)

SLC35D3 delivery from megakaryocyte early endosomes is required for platelet dense granule biogenesis and is differentially defective in Hermansky-Pudlak syndrome models.
Meng R, Wang Y, Yao Y, Zhang Z, Harper DC, Heijnen HF, Sitaram A, Li W, Raposo G, Weiss MJ, Poncz M, Marks MS
Blood120(2)404-14(2012 Jul)

The Slc35d3 gene, encoding an orphan nucleotide sugar transporter, regulates platelet-dense granules.
Chintala S, Tan J, Gautam R, Rusiniak ME, Guo X, Li W, Gahl WA, Huizing M, Spritz RA, Hutton S, Novak EK, Swank RT
Blood109(4)1533-40(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

that SLC35D3 enhanced the formation of the BECN1-ATG14-PIK3C3 complex to induce autophagy
Title: SLC35D3 increases autophagic activity in midbrain dopaminergic neurons by enhancing BECN1-ATG14-PIK3C3 complex formation.

SLC35D3 is depleted in platelets from 2 of 3 Hermansky-Pudlak syndrome models and, when expressed ectopically in melanocytes, SLC35D3 localizes to melanosomes in a manner requiring a HPS-associated protein complex
Title: SLC35D3 delivery from megakaryocyte early endosomes is required for platelet dense granule biogenesis and is differentially defective in Hermansky-Pudlak syndrome models.

Slc35d3 specifically regulates the contents of platelet-dense granules. Unlike HPS or CHS genes, it has no apparent effect on other lysosome-related organelles such as melanosomes or lysosomes
Title: The Slc35d3 gene, encoding an orphan nucleotide sugar transporter, regulates platelet-dense granules.

The following Slc35d3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc35d3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07095	NM_029529.3 Latest version!	Mus musculus solute carrier family 35, member D3 (Slc35d3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu44005	XM_006512916.4 Latest version!	Mus musculus solute carrier family 35, member D3 (Slc35d3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu44005	XM_006512916.3	Mus musculus solute carrier family 35, member D3 (Slc35d3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc35d3 ( NM_029529.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083805.1 Mus musculus solute carrier family 35, member D3 (Slc35d3), mRNA.

Price & Availability↑

CloneID	OMu07095
Clone ID Related Accession (Same CDS sequence)	NM_029529.3
Accession Version	NM_029529.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Mus musculus(house mouse)
Product	solute carrier family 35 member D3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC171277.9. On Apr 30, 2007 this sequence version replaced NM_029529.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK047610.1, AK044973.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCAGC TGTGCCGGGG CCGCGTGCTG GGCATCTCGG TGGCGATCGC GCACGGGGTC 
TTTTCTGGCT CCCTCAACAT CCTGCTCAAG TTCCTCATCA GCCGCTACCA GTTCTCCTTC 
CTGACCCTGG TGCAGTGCCT GACCAGCTCC ACCGCCGCGC TGAGCCTGGA GCTGCTGCGG 
CGCCTGGGGC TCATTGCCGT GCCCCCCTTC GGCCTGAGCC TAGCTCGCTC CTTCGCTGGG 
GTTGCCGTGC TCTCCACGCT GCAGTCCAGT CTCACTCTCT GGTCGCTGCG CGGCCTCAGC 
CTGCCCATGT ACGTAGTCTT CAAGCGCTGC CTGCCCCTAG TCACCATGCT CATCGGTGTG 
CTGGTGCTCA AGAACGGCGC GCCCTCGCCC GGAGTGCTAG CGGCCGTGCT CATCACCACC 
TGCGGCGCCG CCCTGGCAGG AGCTGGCGAC CTGACGGGCG ACCCCATTGG GTACGTAACG 
GGCGTGCTGG CTGTATTGGT GCACGCAGCC TACCTGGTGC TGATCCAGAA GGCGAGCGCG 
GACACGGAGC ACGGGCCTCT CACCGCACAG TATGTGATCG CCGTCTCCGC CACCCCTCTG 
CTGGTCATCT GCTCTTTCGC CAGCACCGAC TCCATCCACG CCTGGACCTT TCCAGGCTGG 
AAGGACCCGG CCATGGTTTC CATATTCGTG GCCTGTATCC TGATCGGCTG TGCCATGAAC 
TTCACCACAC TGCACTGTAC CTACATCAAC TCTGCTGTGA CCACCAGCTT CGTGGGCGTG 
GTGAAGAGCA TCGCCACTAT CACGGTGGGC ATGGTGGCGT TCAGCGACGT GGAGCCCACC 
TCTCTATTCA TTGCTGGCGT TGTGGTGAAC ACCCTGGGCT CCATCATTTA TTGTGTGGCC 
AAATTCTTGG AGACCAGAAG GCAAAGCAAC TATGAAGATC TGGAATCGCA GGCAGAGGGA 
GAGGAGCGAC AGCCAAGTGG GGACCAGTTG CCTTTCGTTA TGGAGGAGCT GCCCGCCAAG 
AGCGGAAATA GCGAGCCAGA GAGTGCGGAG GGGGCAGGTG ACTCCGTGCA GCAAGGTGGG 
CAAGAGTCAA GGGGCAGCAT CAGAGGGATT TCACTGGCGG CTAGGAGTTC AAGAGCAGAA 
GGGCATTCTG ATGAGGTGGG AAGGAGGTCC TTAAAGGACA CTTACTTAGA AGTGTGGAGA 
CTAGTTAGGG GAACTAAGTA CATGAAGAAG GATTATTTGA TGGAAAACGA GGCATTACCC 
AGTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083805.1
CDS	438..1706
Translation	MRQLCRGRVLGISVAIAHGVFSGSLNILLKFLISRYQFSFLTLVQCLTSSTAALSLELLRRLGLIAVPPFGLSLARSFAGVAVLSTLQSSLTLWSLRGLSLPMYVVFKRCLPLVTMLIGVLVLKNGAPSPGVLAAVLITTCGAALAGAGDLTGDPIGYVTGVLAVLVHAAYLVLIQKASADTEHGPLTAQYVIAVSATPLLVICSFASTDSIHAWTFPGWKDPAMVSIFVACILIGCAMNFTTLHCTYINSAVTTSFVGVVKSIATITVGMVAFSDVEPTSLFIAGVVVNTLGSIIYCVAKFLETRRQSNYEDLESQAEGEERQPSGDQLPFVMEELPAKSGNSEPESAEGAGDSVQQGGQESRGSIRGISLAARSSRAEGHSDEVGRRSLKDTYLEVWRLVRGTKYMKKDYLMENEALPSP

Target ORF information:

RefSeq Version	NM_029529.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 35, member D3 (Slc35d3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029529.3

ORF Insert Sequence:

ATGCGGCAGC TGTGCCGGGG CCGCGTGCTG GGCATCTCGG TGGCGATCGC GCACGGGGTC 
TTTTCTGGCT CCCTCAACAT CCTGCTCAAG TTCCTCATCA GCCGCTACCA GTTCTCCTTC 
CTGACCCTGG TGCAGTGCCT GACCAGCTCC ACCGCCGCGC TGAGCCTGGA GCTGCTGCGG 
CGCCTGGGGC TCATTGCCGT GCCCCCCTTC GGCCTGAGCC TAGCTCGCTC CTTCGCTGGG 
GTTGCCGTGC TCTCCACGCT GCAGTCCAGT CTCACTCTCT GGTCGCTGCG CGGCCTCAGC 
CTGCCCATGT ACGTAGTCTT CAAGCGCTGC CTGCCCCTAG TCACCATGCT CATCGGTGTG 
CTGGTGCTCA AGAACGGCGC GCCCTCGCCC GGAGTGCTAG CGGCCGTGCT CATCACCACC 
TGCGGCGCCG CCCTGGCAGG AGCTGGCGAC CTGACGGGCG ACCCCATTGG GTACGTAACG 
GGCGTGCTGG CTGTATTGGT GCACGCAGCC TACCTGGTGC TGATCCAGAA GGCGAGCGCG 
GACACGGAGC ACGGGCCTCT CACCGCACAG TATGTGATCG CCGTCTCCGC CACCCCTCTG 
CTGGTCATCT GCTCTTTCGC CAGCACCGAC TCCATCCACG CCTGGACCTT TCCAGGCTGG 
AAGGACCCGG CCATGGTTTC CATATTCGTG GCCTGTATCC TGATCGGCTG TGCCATGAAC 
TTCACCACAC TGCACTGTAC CTACATCAAC TCTGCTGTGA CCACCAGCTT CGTGGGCGTG 
GTGAAGAGCA TCGCCACTAT CACGGTGGGC ATGGTGGCGT TCAGCGACGT GGAGCCCACC 
TCTCTATTCA TTGCTGGCGT TGTGGTGAAC ACCCTGGGCT CCATCATTTA TTGTGTGGCC 
AAATTCTTGG AGACCAGAAG GCAAAGCAAC TATGAAGATC TGGAATCGCA GGCAGAGGGA 
GAGGAGCGAC AGCCAAGTGG GGACCAGTTG CCTTTCGTTA TGGAGGAGCT GCCCGCCAAG 
AGCGGAAATA GCGAGCCAGA GAGTGCGGAG GGGGCAGGTG ACTCCGTGCA GCAAGGTGGG 
CAAGAGTCAA GGGGCAGCAT CAGAGGGATT TCACTGGCGG CTAGGAGTTC AAGAGCAGAA 
GGGCATTCTG ATGAGGTGGG AAGGAGGTCC TTAAAGGACA CTTACTTAGA AGTGTGGAGA 
CTAGTTAGGG GAACTAAGTA CATGAAGAAG GATTATTTGA TGGAAAACGA GGCATTACCC 
AGTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc35d3 ( XM_006512916.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512979.1 Mus musculus solute carrier family 35, member D3 (Slc35d3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu44005
Clone ID Related Accession (Same CDS sequence)	XM_006512916.3 , XM_006512916.4
Accession Version	XM_006512916.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	solute carrier family 35 member D3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512916.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCAGC TGTGCCGGGG CCGCGTGCTG GGCATCTCGG TGGCGATCGC GCACGGGGTC 
TTTTCTGGCT CCCTCAACAT CCTGCTCAAG TTCCTCATCA GCCGCTACCA GTTCTCCTTC 
CTGACCCTGG TGCAGTGCCT GACCAGCTCC ACCGCCGCGC TGAGCCTGGA GCTGCTGCGG 
CGCCTGGGGC TCATTGCCGT GCCCCCCTTC GGCCTGAGCC TAGCTCGCTC CTTCGCTGGG 
GTTGCCGTGC TCTCCACGCT GCAGTCCAGT CTCACTCTCT GGTCGCTGCG CGGCCTCAGC 
CTGCCCATGT ACGTAGTCTT CAAGCGCTGC CTGCCCCTAG TCACCATGCT CATCGGTGTG 
CTGGTGCTCA AGAACGGCGC GCCCTCGCCC GGAGTGCTAG CGGCCGTGCT CATCACCACC 
TGCGGCGCCG CCCTGGCAGC CCCTTCCTCA GGAGGGATGG AGAGTCGCCA GCGCAGAGAA 
CTGGAGTCCA GCCCTGCACT GAGCCGGAAG GAAGGGGGCC GGGAACTTCC TCGGATCACG 
CGGGGTTGGG GAGCTGGAGA GACGGGAGTG AAGCCTGAGC AACCTGGGCT CAGTACCCGG 
GCCCTAGGCA AAAGGAACCG CCCCGGGGCT CAAGGGCAGG AGGGGCGGGG AGGACCCCCG 
GGGAATCCAG CTGCGGGCAG AGGGGAAACA CTCTTTCCGG GGCCGTGCCC GCACTCGGTT 
GAGGCCGCTA GCTGGGGTTC CCTGTCCCTT CCGGTGCCCA CGGGGCGGGA ACCACCCTCC 
CAGGTGGCCT CCGTGCAGGC TTCAGGGACC CCAAGGAGCC TACCCAGAGA GCATCTGATC 
TCCCCCTGGC CTTCGCCCTG GCTAGAGGAA GACCGGGTAC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512979.1
CDS	1342..2226
Translation	MRQLCRGRVLGISVAIAHGVFSGSLNILLKFLISRYQFSFLTLVQCLTSSTAALSLELLRRLGLIAVPPFGLSLARSFAGVAVLSTLQSSLTLWSLRGLSLPMYVVFKRCLPLVTMLIGVLVLKNGAPSPGVLAAVLITTCGAALAAPSSGGMESRQRRELESSPALSRKEGGRELPRITRGWGAGETGVKPEQPGLSTRALGKRNRPGAQGQEGRGGPPGNPAAGRGETLFPGPCPHSVEAASWGSLSLPVPTGREPPSQVASVQASGTPRSLPREHLISPWPSPWLEEDRVH

Target ORF information:

RefSeq Version	XM_006512916.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 35, member D3 (Slc35d3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512916.3

ORF Insert Sequence:

ATGCGGCAGC TGTGCCGGGG CCGCGTGCTG GGCATCTCGG TGGCGATCGC GCACGGGGTC 
TTTTCTGGCT CCCTCAACAT CCTGCTCAAG TTCCTCATCA GCCGCTACCA GTTCTCCTTC 
CTGACCCTGG TGCAGTGCCT GACCAGCTCC ACCGCCGCGC TGAGCCTGGA GCTGCTGCGG 
CGCCTGGGGC TCATTGCCGT GCCCCCCTTC GGCCTGAGCC TAGCTCGCTC CTTCGCTGGG 
GTTGCCGTGC TCTCCACGCT GCAGTCCAGT CTCACTCTCT GGTCGCTGCG CGGCCTCAGC 
CTGCCCATGT ACGTAGTCTT CAAGCGCTGC CTGCCCCTAG TCACCATGCT CATCGGTGTG 
CTGGTGCTCA AGAACGGCGC GCCCTCGCCC GGAGTGCTAG CGGCCGTGCT CATCACCACC 
TGCGGCGCCG CCCTGGCAGC CCCTTCCTCA GGAGGGATGG AGAGTCGCCA GCGCAGAGAA 
CTGGAGTCCA GCCCTGCACT GAGCCGGAAG GAAGGGGGCC GGGAACTTCC TCGGATCACG 
CGGGGTTGGG GAGCTGGAGA GACGGGAGTG AAGCCTGAGC AACCTGGGCT CAGTACCCGG 
GCCCTAGGCA AAAGGAACCG CCCCGGGGCT CAAGGGCAGG AGGGGCGGGG AGGACCCCCG 
GGGAATCCAG CTGCGGGCAG AGGGGAAACA CTCTTTCCGG GGCCGTGCCC GCACTCGGTT 
GAGGCCGCTA GCTGGGGTTC CCTGTCCCTT CCGGTGCCCA CGGGGCGGGA ACCACCCTCC 
CAGGTGGCCT CCGTGCAGGC TTCAGGGACC CCAAGGAGCC TACCCAGAGA GCATCTGATC 
TCCCCCTGGC CTTCGCCCTG GCTAGAGGAA GACCGGGTAC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc35d3 ( XM_006512916.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512979.1 Mus musculus solute carrier family 35, member D3 (Slc35d3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu44005
Clone ID Related Accession (Same CDS sequence)	XM_006512916.3 , XM_006512916.4
Accession Version	XM_006512916.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 35 member D3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512916.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCAGC TGTGCCGGGG CCGCGTGCTG GGCATCTCGG TGGCGATCGC GCACGGGGTC 
TTTTCTGGCT CCCTCAACAT CCTGCTCAAG TTCCTCATCA GCCGCTACCA GTTCTCCTTC 
CTGACCCTGG TGCAGTGCCT GACCAGCTCC ACCGCCGCGC TGAGCCTGGA GCTGCTGCGG 
CGCCTGGGGC TCATTGCCGT GCCCCCCTTC GGCCTGAGCC TAGCTCGCTC CTTCGCTGGG 
GTTGCCGTGC TCTCCACGCT GCAGTCCAGT CTCACTCTCT GGTCGCTGCG CGGCCTCAGC 
CTGCCCATGT ACGTAGTCTT CAAGCGCTGC CTGCCCCTAG TCACCATGCT CATCGGTGTG 
CTGGTGCTCA AGAACGGCGC GCCCTCGCCC GGAGTGCTAG CGGCCGTGCT CATCACCACC 
TGCGGCGCCG CCCTGGCAGC CCCTTCCTCA GGAGGGATGG AGAGTCGCCA GCGCAGAGAA 
CTGGAGTCCA GCCCTGCACT GAGCCGGAAG GAAGGGGGCC GGGAACTTCC TCGGATCACG 
CGGGGTTGGG GAGCTGGAGA GACGGGAGTG AAGCCTGAGC AACCTGGGCT CAGTACCCGG 
GCCCTAGGCA AAAGGAACCG CCCCGGGGCT CAAGGGCAGG AGGGGCGGGG AGGACCCCCG 
GGGAATCCAG CTGCGGGCAG AGGGGAAACA CTCTTTCCGG GGCCGTGCCC GCACTCGGTT 
GAGGCCGCTA GCTGGGGTTC CCTGTCCCTT CCGGTGCCCA CGGGGCGGGA ACCACCCTCC 
CAGGTGGCCT CCGTGCAGGC TTCAGGGACC CCAAGGAGCC TACCCAGAGA GCATCTGATC 
TCCCCCTGGC CTTCGCCCTG GCTAGAGGAA GACCGGGTAC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512979.1
CDS	5613..6497
Translation	MRQLCRGRVLGISVAIAHGVFSGSLNILLKFLISRYQFSFLTLVQCLTSSTAALSLELLRRLGLIAVPPFGLSLARSFAGVAVLSTLQSSLTLWSLRGLSLPMYVVFKRCLPLVTMLIGVLVLKNGAPSPGVLAAVLITTCGAALAAPSSGGMESRQRRELESSPALSRKEGGRELPRITRGWGAGETGVKPEQPGLSTRALGKRNRPGAQGQEGRGGPPGNPAAGRGETLFPGPCPHSVEAASWGSLSLPVPTGREPPSQVASVQASGTPRSLPREHLISPWPSPWLEEDRVH

Target ORF information:

RefSeq Version	XM_006512916.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 35, member D3 (Slc35d3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512916.4

ORF Insert Sequence:

ATGCGGCAGC TGTGCCGGGG CCGCGTGCTG GGCATCTCGG TGGCGATCGC GCACGGGGTC 
TTTTCTGGCT CCCTCAACAT CCTGCTCAAG TTCCTCATCA GCCGCTACCA GTTCTCCTTC 
CTGACCCTGG TGCAGTGCCT GACCAGCTCC ACCGCCGCGC TGAGCCTGGA GCTGCTGCGG 
CGCCTGGGGC TCATTGCCGT GCCCCCCTTC GGCCTGAGCC TAGCTCGCTC CTTCGCTGGG 
GTTGCCGTGC TCTCCACGCT GCAGTCCAGT CTCACTCTCT GGTCGCTGCG CGGCCTCAGC 
CTGCCCATGT ACGTAGTCTT CAAGCGCTGC CTGCCCCTAG TCACCATGCT CATCGGTGTG 
CTGGTGCTCA AGAACGGCGC GCCCTCGCCC GGAGTGCTAG CGGCCGTGCT CATCACCACC 
TGCGGCGCCG CCCTGGCAGC CCCTTCCTCA GGAGGGATGG AGAGTCGCCA GCGCAGAGAA 
CTGGAGTCCA GCCCTGCACT GAGCCGGAAG GAAGGGGGCC GGGAACTTCC TCGGATCACG 
CGGGGTTGGG GAGCTGGAGA GACGGGAGTG AAGCCTGAGC AACCTGGGCT CAGTACCCGG 
GCCCTAGGCA AAAGGAACCG CCCCGGGGCT CAAGGGCAGG AGGGGCGGGG AGGACCCCCG 
GGGAATCCAG CTGCGGGCAG AGGGGAAACA CTCTTTCCGG GGCCGTGCCC GCACTCGGTT 
GAGGCCGCTA GCTGGGGTTC CCTGTCCCTT CCGGTGCCCA CGGGGCGGGA ACCACCCTCC 
CAGGTGGCCT CCGTGCAGGC TTCAGGGACC CCAAGGAGCC TACCCAGAGA GCATCTGATC 
TCCCCCTGGC CTTCGCCCTG GCTAGAGGAA GACCGGGTAC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SLC35D3 increases autophagic activity in midbrain dopaminergic neurons by enhancing BECN1-ATG14-PIK3C3 complex formation.
Autophagy12(7)1168-79(2016 Jul)
Wei ZB,Yuan YF,Jaouen F,Ma MS,Hao CJ,Zhang Z,Chen Q,Yuan Z,Yu L,Beurrier C,Li W

Mutation of SLC35D3 causes metabolic syndrome by impairing dopamine signaling in striatal D1 neurons.
PLoS genetics10(2)e1004124(2014 Feb)
Zhang Z,Hao CJ,Li CG,Zang DJ,Zhao J,Li XN,Wei AH,Wei ZB,Yang L,He X,Zhen XC,Gao X,Speakman JR,Li W

Dual role for Islet-1 in promoting striatonigral and repressing striatopallidal genetic programs to specify striatonigral cell identity.
Proceedings of the National Academy of Sciences of the United States of America111(1)E168-77(2014 Jan)
Lu KM,Evans SM,Hirano S,Liu FC

SLC35D3 delivery from megakaryocyte early endosomes is required for platelet dense granule biogenesis and is differentially defective in Hermansky-Pudlak syndrome models.
Blood120(2)404-14(2012 Jul)
Meng R,Wang Y,Yao Y,Zhang Z,Harper DC,Heijnen HF,Sitaram A,Li W,Raposo G,Weiss MJ,Poncz M,Marks MS

The Slc35d3 gene, encoding an orphan nucleotide sugar transporter, regulates platelet-dense granules.
Blood109(4)1533-40(2007 Feb)
Chintala S,Tan J,Gautam R,Rusiniak ME,Guo X,Li W,Gahl WA,Huizing M,Spritz RA,Hutton S,Novak EK,Swank RT