Oga cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Oga
Entrez Gene ID	76055
Full Name	meningioma expressed antigen 5 (hyaluronidase)
Synonyms	Hy5,Ncoat,OGA
General protein information	Preferred Names meningioma expressed antigen 5 (hyaluronidase) Names protein O-GlcNAcase N-acetyl-beta-D-glucosaminidase N-acetyl-beta-glucosaminidase beta-N-acetylhexosaminidase beta-hexosaminidase bifunctional protein NCOAT meningioma-expressed antigen 5 nuclear cytoplasmic O-GlcNAcase and acetyltransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 C3\|19 38.75 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017318305.1	XP_017173794.1	protein O-GlcNAcase isoform X1
XM_017318305.2	XP_017173794.1	protein O-GlcNAcase isoform X1
NM_023799.3	NP_076288.1	protein O-GlcNAcase
NM_023799.4	NP_076288.1	protein O-GlcNAcase

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mgea5	NP_076288.1
Bos taurus (cattle)	MGEA5	NP_001193377.1
Danio rerio (zebrafish)	mgea5	XP_002664185.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002287	XP_307891.5
Caenorhabditis elegans (roundworm)	oga-1	NP_001123184.1
Xenopus tropicalis (tropical clawed frog)	mgea5	NP_001120363.1
Rattus norvegicus (Norway rat)	Mgea5	NP_571979.1
Pan troglodytes (chimpanzee)	MGEA5	XP_507996.3
Canis lupus familiaris (dog)	MGEA5	XP_534996.2
Gallus gallus (chicken)	MGEA5	NP_001034394.1
Homo sapiens (human)	MGEA5	NP_036347.1
Drosophila melanogaster (fruit fly)	Oga	NP_650956.1

Gene Ontology
Bibliography

Related articles in PubMed

O-GlcNAc is required for the survival of primed pluripotent stem cells and their reversion to the na?ve state.
Miura T, Nishihara S
Biochemical and biophysical research communications480(4)655-661(2016 11)

O-Linked N-Acetylglucosamine (O-GlcNAc) Transferase and O-GlcNAcase Interact with Mi2? Protein at the A?-Globin Promoter.
Zhang Z, Costa FC, Tan EP, Bushue N, DiTacchio L, Costello CE, McComb ME, Whelan SA, Peterson KR, Slawson C
The Journal of biological chemistry291(30)15628-40(2016 07)

Obesity resistance and increased energy expenditure by white adipose tissue browning in Oga(+/-) mice.
Yang YR, Jang HJ, Choi SS, Lee YH, Lee GH, Seo YK, Choi JH, Park D, Koh A, Kim IS, Lee H, Ryu SH, Suh PG
Diabetologia58(12)2867-76(2015 Dec)

E2F1 Transcription Factor Regulates O-linked N-acetylglucosamine (O-GlcNAc) Transferase and O-GlcNAcase Expression.
Muthusamy S, Hong KU, Dassanayaka S, Hamid T, Jones SP
The Journal of biological chemistry290(52)31013-24(2015 Dec)

O-GlcNAc cycling enzymes control vascular development of the placenta by modulating the levels of HIF-1?.
Yang YR, Jang HJ, Lee YH, Kim IS, Lee H, Ryu SH, Suh PG
Placenta36(10)1063-8(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Furthermore, both Ogt and Oga were required for the reversion from primed ESD-EpiSCs to naive rESCs. These findings indicate that O-GlcNAcylation plays an important role in the survival of primed ESD-EpiSCs and in their reversion to naive rESCs.
Title: O-GlcNAc is required for the survival of primed pluripotent stem cells and their reversion to the na?ve state.

the O-linked N-acetylglucosamine (O-GlcNAc) processing enzymes, O-GlcNAc-transferase (OGT) and O-GlcNAcase (OGA), interact with the (A)gamma-globin promoter at the -566 GATA repressor site
Title: O-Linked N-Acetylglucosamine (O-GlcNAc) Transferase and O-GlcNAcase Interact with Mi2? Protein at the A?-Globin Promoter.

Oga(+/-) mice resist high-fat diet-induced obesity with ameliorated hepatic steatosis and improved glucose metabolism
Title: Obesity resistance and increased energy expenditure by white adipose tissue browning in Oga(+/-) mice.

E2F1 negatively regulates both Ogt and Mgea5 expression in an Rb1 protein-dependent manner.
Title: E2F1 Transcription Factor Regulates O-linked N-acetylglucosamine (O-GlcNAc) Transferase and O-GlcNAcase Expression.

plays a critical role in placental vasculogenesis by modulating HIF-1alpha stabilization
Title: O-GlcNAc cycling enzymes control vascular development of the placenta by modulating the levels of HIF-1?.

The following Oga gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Oga cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07077	NM_023799.4 Latest version!	Mus musculus O-GlcNAcase (Oga), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$538.30 $769.00
OMu80024	XM_017318305.2 Latest version!	Mus musculus O-GlcNAcase (Oga), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu80024	XM_017318305.1	Mus musculus meningioma expressed antigen 5 (hyaluronidase) (Mgea5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu07077	NM_023799.3	Mus musculus meningioma expressed antigen 5 (hyaluronidase) (Mgea5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$538.30 $769.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Oga ( NM_023799.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_076288.1 Mus musculus meningioma expressed antigen 5 (hyaluronidase) (Mgea5), mRNA.

Price & Availability↑

CloneID	OMu07077
Clone ID Related Accession (Same CDS sequence)	NM_023799.3 , NM_023799.4
Accession Version	NM_023799.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-22
Organism	Mus musculus(House mouse)
Product	protein O-GlcNAcase
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC149086.4. On Nov 17, 2006 this sequence version replaced NM_023799.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054821.1, AK129188.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTGCAGA AGGAGAGCCA GGCGGCGCTG GAGGAGCGGG AGAGCGAGCG CAACGCCAAC 
CCTGCCGCCG CCTCGGGGGC TTCGTTGGAG CAGTCGGTAG CTCCCGCCCC CGGAGAAGAT 
AACCCATCGG GAGCCGGGGC TGCGGCCGTG GTCGGGGCGG CCGGAGGGGC CCGCAGGTTC 
CTGTGCGGTG TCGTGGAAGG GTTTTATGGA AGACCTTGGG TTATGGAGCA GAGAAAAGAG 
CTCTTTAGAA GGCTTCAGAA ATGGGAATTA AACACATACT TGTATGCTCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AAGAAGCTGA GCAACTTATG 
ACTCTGATCT CTGCTGCACG AGAATATGAA ATAGAATTCA TCTATGCTAT TTCGCCAGGA 
CTAGATATCA CTTTTTCTAA CCCCAAGGAA GTATCAACGT TGAAACGCAA ATTGGACCAG 
GTCTCTCAGT TTGGGTGCAG GTCGTTTGCT TTGCTTTTTG ATGATATTGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT GTTCAGTTCT TTTGCACATG CACAAGTTTC CATCACCAAT 
GAAATTTATC AGTACCTGGG AGAGCCAGAA ACCTTCCTCT TCTGCCCTAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTATCTCAG TCTCCTTACT TGAGGACTGT GGGTGAGAAG 
CTTCTACCTG GAATTGAGGT GCTTTGGACA GGTCCAAAAG TTGTTTCAAA AGAAATTCCA 
GTGGAATCTA TTGAAGAAGT TTCTAAGATC ATTAAGAGAG CTCCAGTGAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGG CTGTTTCTGG GTCCCTACAA AGGAAGATCC 
ACAGAGCTCA TCCCACGGTT AAAGGGAGTA CTAACCAATC CAAATTGTGA ATTTGAAGCT 
AACTACGTTG CCATACACAC CCTTGCCACT TGGTACAAGT CAAACATGAA CGGAGTGAGG 
AAGGATGTGG TGATGACTGA CAGTGAAGAC AGTACTGTAT CAATACAGAT AAAGTTAGAA 
AATGAAGGCA GTGACGAAGA CATTGAGACT GATGTTCTCT ACAGCCCACA GATGGCTTTA 
AAGCTGGCTT TAACTGAGTG GCTGCAGGAG TTCGGTGTCC CTCATCAGTA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAGACAAGT GTAGTTGATG GGACTCCCTT AGTTGCTGCA 
CCCTCTTTAA ATGCCACAAC TGTAGTTACA ACAGTTTACC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCTT TGAGTGGTGA ACCCTCAGTT CTGACCAAGG AAGAGGAAAA GAAACAGCCA 
GATGAGGAAC CCATGGATAT GGTGGTCGAA AAGCAAGAAG AAGCAGAGCA CAAGAATGAC 
AACCAGATAC TAACTGAGAT TGTTGAAGCG AAGATGGCAG AGGAGTTGAG ACCAATGGAC 
ACGGATAAGG AGAGCATGGC TGAGTCCAAA TCCCCAGAGA TGTCCATGCA GGAAGACTGC 
ATTCCTGACG TGGCTCCTAT GCAGACAGAT GAGCAAACAC AGAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTCT GTACACCGCA GAGCCTGTGA CTCTGGAGGA CTTACAGTTA 
CTCGCTGACC TGTTCTACCT TCCTTACGAG CACGGGCCCA AAGGAGCGCA GATGCTACGG 
GAATTCCAGT GGCTTCGAGC AAACAGCAGT GTTGTCAGTG TCAACTGCAA AGGGAAAGAC 
TCAGAAAAAA TAGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGCACTA 
GTGATGGGGA TGTTCACCCG GCTTTCCAAC TGTGCCAACA GGACCATCCT TTATGACATG 
TACTCCTATG TGTGGGACAT CAAGAGTATA ATGTCTATGG TGAAATCTTT TGTGCAGTGG 
TTAGGGTGTC GTAGTCACTC TTCAGCACAG TTCTTAATTG GAGACCAGGA GCCCTGGGCC 
TTTAGAGGTG GTCTAGCAGG AGAGTTCCAG CGTTTGCTGC CAATTGATGG AGCAAATGAT 
CTCTTTTTTC AACCACCCCC ACTGACTCCA ACCTCCAAAG TTTATACTAT CAGACCTTAT 
TTCCCTAAAG ATGAGGCTTC CGTGTACAAG ATTTGCAGAG AAATGTATGA TGATGGAGTG 
GGTTTCCCTT TTCAAAGTCA ACCTGACCTT ATTGGAGACA AGTTAGTAGG AGGACTGCTC 
TCCCTCAGCC TGGATTACTG CTTCGTCCTA GAAGATGAAG ATGGCATTTG TGGTTATGCC 
TTAGGCACAG TAGATGTGAC CCCCTTCATT AAAAAGTGTA AAATTTCCTG GATTCCCTTC 
ATGCAAGAGA AGTATACCAA GCCAAATGGT GACAAAGAGC TCTCAGAGGC TGAGAAAATA 
ATGTTGAGTT TCCATGAAGA GCAGGAGGTG TTGCCAGAAA CTTTCCTTGC CAACTTCCCT 
TCTCTGATAA AAATGGATAT TCATAAAAAA GTGACTGACC CGAGTGTTGC CAAAAGCATG 
ATGGCTTGCC TCCTGTCTTC ACTGAAGGCC AATGGCTCCC GGGGGGCTTT CTGTGAAGTA 
AGACCAGATG ATAAAAGGAT TCTGGAATTT TACAGCAAGT TAGGCTGTTT TGAAATTGCA 
AAAATGGAAG GATTTCCAAA GGATGTGGTT ATACTTGGTC GAAGCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076288.1
CDS	162..2912
Translation	MVQKESQAALEERESERNANPAAASGASLEQSVAPAPGEDNPSGAGAAAVVGAAGGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKTSVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPSVLTKEEEKKQPDEEPMDMVVEKQEEAEHKNDNQILTEIVEAKMAEELRPMDTDKESMAESKSPEMSMQEDCIPDVAPMQTDEQTQKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCALVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGANDLFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGFPFQSQPDLIGDKLVGGLLSLSLDYCFVLEDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGRSL

Target ORF information:

RefSeq Version	NM_023799.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus meningioma expressed antigen 5 (hyaluronidase) (Mgea5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023799.3

ORF Insert Sequence:

ATGGTGCAGA AGGAGAGCCA GGCGGCGCTG GAGGAGCGGG AGAGCGAGCG CAACGCCAAC 
CCTGCCGCCG CCTCGGGGGC TTCGTTGGAG CAGTCGGTAG CTCCCGCCCC CGGAGAAGAT 
AACCCATCGG GAGCCGGGGC TGCGGCCGTG GTCGGGGCGG CCGGAGGGGC CCGCAGGTTC 
CTGTGCGGTG TCGTGGAAGG GTTTTATGGA AGACCTTGGG TTATGGAGCA GAGAAAAGAG 
CTCTTTAGAA GGCTTCAGAA ATGGGAATTA AACACATACT TGTATGCTCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AAGAAGCTGA GCAACTTATG 
ACTCTGATCT CTGCTGCACG AGAATATGAA ATAGAATTCA TCTATGCTAT TTCGCCAGGA 
CTAGATATCA CTTTTTCTAA CCCCAAGGAA GTATCAACGT TGAAACGCAA ATTGGACCAG 
GTCTCTCAGT TTGGGTGCAG GTCGTTTGCT TTGCTTTTTG ATGATATTGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT GTTCAGTTCT TTTGCACATG CACAAGTTTC CATCACCAAT 
GAAATTTATC AGTACCTGGG AGAGCCAGAA ACCTTCCTCT TCTGCCCTAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTATCTCAG TCTCCTTACT TGAGGACTGT GGGTGAGAAG 
CTTCTACCTG GAATTGAGGT GCTTTGGACA GGTCCAAAAG TTGTTTCAAA AGAAATTCCA 
GTGGAATCTA TTGAAGAAGT TTCTAAGATC ATTAAGAGAG CTCCAGTGAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGG CTGTTTCTGG GTCCCTACAA AGGAAGATCC 
ACAGAGCTCA TCCCACGGTT AAAGGGAGTA CTAACCAATC CAAATTGTGA ATTTGAAGCT 
AACTACGTTG CCATACACAC CCTTGCCACT TGGTACAAGT CAAACATGAA CGGAGTGAGG 
AAGGATGTGG TGATGACTGA CAGTGAAGAC AGTACTGTAT CAATACAGAT AAAGTTAGAA 
AATGAAGGCA GTGACGAAGA CATTGAGACT GATGTTCTCT ACAGCCCACA GATGGCTTTA 
AAGCTGGCTT TAACTGAGTG GCTGCAGGAG TTCGGTGTCC CTCATCAGTA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAGACAAGT GTAGTTGATG GGACTCCCTT AGTTGCTGCA 
CCCTCTTTAA ATGCCACAAC TGTAGTTACA ACAGTTTACC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCTT TGAGTGGTGA ACCCTCAGTT CTGACCAAGG AAGAGGAAAA GAAACAGCCA 
GATGAGGAAC CCATGGATAT GGTGGTCGAA AAGCAAGAAG AAGCAGAGCA CAAGAATGAC 
AACCAGATAC TAACTGAGAT TGTTGAAGCG AAGATGGCAG AGGAGTTGAG ACCAATGGAC 
ACGGATAAGG AGAGCATGGC TGAGTCCAAA TCCCCAGAGA TGTCCATGCA GGAAGACTGC 
ATTCCTGACG TGGCTCCTAT GCAGACAGAT GAGCAAACAC AGAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTCT GTACACCGCA GAGCCTGTGA CTCTGGAGGA CTTACAGTTA 
CTCGCTGACC TGTTCTACCT TCCTTACGAG CACGGGCCCA AAGGAGCGCA GATGCTACGG 
GAATTCCAGT GGCTTCGAGC AAACAGCAGT GTTGTCAGTG TCAACTGCAA AGGGAAAGAC 
TCAGAAAAAA TAGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGCACTA 
GTGATGGGGA TGTTCACCCG GCTTTCCAAC TGTGCCAACA GGACCATCCT TTATGACATG 
TACTCCTATG TGTGGGACAT CAAGAGTATA ATGTCTATGG TGAAATCTTT TGTGCAGTGG 
TTAGGGTGTC GTAGTCACTC TTCAGCACAG TTCTTAATTG GAGACCAGGA GCCCTGGGCC 
TTTAGAGGTG GTCTAGCAGG AGAGTTCCAG CGTTTGCTGC CAATTGATGG AGCAAATGAT 
CTCTTTTTTC AACCACCCCC ACTGACTCCA ACCTCCAAAG TTTATACTAT CAGACCTTAT 
TTCCCTAAAG ATGAGGCTTC CGTGTACAAG ATTTGCAGAG AAATGTATGA TGATGGAGTG 
GGTTTCCCTT TTCAAAGTCA ACCTGACCTT ATTGGAGACA AGTTAGTAGG AGGACTGCTC 
TCCCTCAGCC TGGATTACTG CTTCGTCCTA GAAGATGAAG ATGGCATTTG TGGTTATGCC 
TTAGGCACAG TAGATGTGAC CCCCTTCATT AAAAAGTGTA AAATTTCCTG GATTCCCTTC 
ATGCAAGAGA AGTATACCAA GCCAAATGGT GACAAAGAGC TCTCAGAGGC TGAGAAAATA 
ATGTTGAGTT TCCATGAAGA GCAGGAGGTG TTGCCAGAAA CTTTCCTTGC CAACTTCCCT 
TCTCTGATAA AAATGGATAT TCATAAAAAA GTGACTGACC CGAGTGTTGC CAAAAGCATG 
ATGGCTTGCC TCCTGTCTTC ACTGAAGGCC AATGGCTCCC GGGGGGCTTT CTGTGAAGTA 
AGACCAGATG ATAAAAGGAT TCTGGAATTT TACAGCAAGT TAGGCTGTTT TGAAATTGCA 
AAAATGGAAG GATTTCCAAA GGATGTGGTT ATACTTGGTC GAAGCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Oga ( NM_023799.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_076288.1 Mus musculus O-GlcNAcase (Oga), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07077
Clone ID Related Accession (Same CDS sequence)	NM_023799.3 , NM_023799.4
Accession Version	NM_023799.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	protein O-GlcNAcase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC149086.4. On Oct 14, 2017 this sequence version replaced NM_023799.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK129188.1, SRR1660815.198586.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGTGCAGA AGGAGAGCCA GGCGGCGCTG GAGGAGCGGG AGAGCGAGCG CAACGCCAAC 
CCTGCCGCCG CCTCGGGGGC TTCGTTGGAG CAGTCGGTAG CTCCCGCCCC CGGAGAAGAT 
AACCCATCGG GAGCCGGGGC TGCGGCCGTG GTCGGGGCGG CCGGAGGGGC CCGCAGGTTC 
CTGTGCGGTG TCGTGGAAGG GTTTTATGGA AGACCTTGGG TTATGGAGCA GAGAAAAGAG 
CTCTTTAGAA GGCTTCAGAA ATGGGAATTA AACACATACT TGTATGCTCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AAGAAGCTGA GCAACTTATG 
ACTCTGATCT CTGCTGCACG AGAATATGAA ATAGAATTCA TCTATGCTAT TTCGCCAGGA 
CTAGATATCA CTTTTTCTAA CCCCAAGGAA GTATCAACGT TGAAACGCAA ATTGGACCAG 
GTCTCTCAGT TTGGGTGCAG GTCGTTTGCT TTGCTTTTTG ATGATATTGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT GTTCAGTTCT TTTGCACATG CACAAGTTTC CATCACCAAT 
GAAATTTATC AGTACCTGGG AGAGCCAGAA ACCTTCCTCT TCTGCCCTAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTATCTCAG TCTCCTTACT TGAGGACTGT GGGTGAGAAG 
CTTCTACCTG GAATTGAGGT GCTTTGGACA GGTCCAAAAG TTGTTTCAAA AGAAATTCCA 
GTGGAATCTA TTGAAGAAGT TTCTAAGATC ATTAAGAGAG CTCCAGTGAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGG CTGTTTCTGG GTCCCTACAA AGGAAGATCC 
ACAGAGCTCA TCCCACGGTT AAAGGGAGTA CTAACCAATC CAAATTGTGA ATTTGAAGCT 
AACTACGTTG CCATACACAC CCTTGCCACT TGGTACAAGT CAAACATGAA CGGAGTGAGG 
AAGGATGTGG TGATGACTGA CAGTGAAGAC AGTACTGTAT CAATACAGAT AAAGTTAGAA 
AATGAAGGCA GTGACGAAGA CATTGAGACT GATGTTCTCT ACAGCCCACA GATGGCTTTA 
AAGCTGGCTT TAACTGAGTG GCTGCAGGAG TTCGGTGTCC CTCATCAGTA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAGACAAGT GTAGTTGATG GGACTCCCTT AGTTGCTGCA 
CCCTCTTTAA ATGCCACAAC TGTAGTTACA ACAGTTTACC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCTT TGAGTGGTGA ACCCTCAGTT CTGACCAAGG AAGAGGAAAA GAAACAGCCA 
GATGAGGAAC CCATGGATAT GGTGGTCGAA AAGCAAGAAG AAGCAGAGCA CAAGAATGAC 
AACCAGATAC TAACTGAGAT TGTTGAAGCG AAGATGGCAG AGGAGTTGAG ACCAATGGAC 
ACGGATAAGG AGAGCATGGC TGAGTCCAAA TCCCCAGAGA TGTCCATGCA GGAAGACTGC 
ATTCCTGACG TGGCTCCTAT GCAGACAGAT GAGCAAACAC AGAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTCT GTACACCGCA GAGCCTGTGA CTCTGGAGGA CTTACAGTTA 
CTCGCTGACC TGTTCTACCT TCCTTACGAG CACGGGCCCA AAGGAGCGCA GATGCTACGG 
GAATTCCAGT GGCTTCGAGC AAACAGCAGT GTTGTCAGTG TCAACTGCAA AGGGAAAGAC 
TCAGAAAAAA TAGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGCACTA 
GTGATGGGGA TGTTCACCCG GCTTTCCAAC TGTGCCAACA GGACCATCCT TTATGACATG 
TACTCCTATG TGTGGGACAT CAAGAGTATA ATGTCTATGG TGAAATCTTT TGTGCAGTGG 
TTAGGGTGTC GTAGTCACTC TTCAGCACAG TTCTTAATTG GAGACCAGGA GCCCTGGGCC 
TTTAGAGGTG GTCTAGCAGG AGAGTTCCAG CGTTTGCTGC CAATTGATGG AGCAAATGAT 
CTCTTTTTTC AACCACCCCC ACTGACTCCA ACCTCCAAAG TTTATACTAT CAGACCTTAT 
TTCCCTAAAG ATGAGGCTTC CGTGTACAAG ATTTGCAGAG AAATGTATGA TGATGGAGTG 
GGTTTCCCTT TTCAAAGTCA ACCTGACCTT ATTGGAGACA AGTTAGTAGG AGGACTGCTC 
TCCCTCAGCC TGGATTACTG CTTCGTCCTA GAAGATGAAG ATGGCATTTG TGGTTATGCC 
TTAGGCACAG TAGATGTGAC CCCCTTCATT AAAAAGTGTA AAATTTCCTG GATTCCCTTC 
ATGCAAGAGA AGTATACCAA GCCAAATGGT GACAAAGAGC TCTCAGAGGC TGAGAAAATA 
ATGTTGAGTT TCCATGAAGA GCAGGAGGTG TTGCCAGAAA CTTTCCTTGC CAACTTCCCT 
TCTCTGATAA AAATGGATAT TCATAAAAAA GTGACTGACC CGAGTGTTGC CAAAAGCATG 
ATGGCTTGCC TCCTGTCTTC ACTGAAGGCC AATGGCTCCC GGGGGGCTTT CTGTGAAGTA 
AGACCAGATG ATAAAAGGAT TCTGGAATTT TACAGCAAGT TAGGCTGTTT TGAAATTGCA 
AAAATGGAAG GATTTCCAAA GGATGTGGTT ATACTTGGTC GAAGCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076288.1
CDS	424..3174
Translation	MVQKESQAALEERESERNANPAAASGASLEQSVAPAPGEDNPSGAGAAAVVGAAGGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKTSVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPSVLTKEEEKKQPDEEPMDMVVEKQEEAEHKNDNQILTEIVEAKMAEELRPMDTDKESMAESKSPEMSMQEDCIPDVAPMQTDEQTQKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCALVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGANDLFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGFPFQSQPDLIGDKLVGGLLSLSLDYCFVLEDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGRSL

Target ORF information:

RefSeq Version	NM_023799.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus O-GlcNAcase (Oga), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023799.4

ORF Insert Sequence:

ATGGTGCAGA AGGAGAGCCA GGCGGCGCTG GAGGAGCGGG AGAGCGAGCG CAACGCCAAC 
CCTGCCGCCG CCTCGGGGGC TTCGTTGGAG CAGTCGGTAG CTCCCGCCCC CGGAGAAGAT 
AACCCATCGG GAGCCGGGGC TGCGGCCGTG GTCGGGGCGG CCGGAGGGGC CCGCAGGTTC 
CTGTGCGGTG TCGTGGAAGG GTTTTATGGA AGACCTTGGG TTATGGAGCA GAGAAAAGAG 
CTCTTTAGAA GGCTTCAGAA ATGGGAATTA AACACATACT TGTATGCTCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AAGAAGCTGA GCAACTTATG 
ACTCTGATCT CTGCTGCACG AGAATATGAA ATAGAATTCA TCTATGCTAT TTCGCCAGGA 
CTAGATATCA CTTTTTCTAA CCCCAAGGAA GTATCAACGT TGAAACGCAA ATTGGACCAG 
GTCTCTCAGT TTGGGTGCAG GTCGTTTGCT TTGCTTTTTG ATGATATTGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT GTTCAGTTCT TTTGCACATG CACAAGTTTC CATCACCAAT 
GAAATTTATC AGTACCTGGG AGAGCCAGAA ACCTTCCTCT TCTGCCCTAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTATCTCAG TCTCCTTACT TGAGGACTGT GGGTGAGAAG 
CTTCTACCTG GAATTGAGGT GCTTTGGACA GGTCCAAAAG TTGTTTCAAA AGAAATTCCA 
GTGGAATCTA TTGAAGAAGT TTCTAAGATC ATTAAGAGAG CTCCAGTGAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGG CTGTTTCTGG GTCCCTACAA AGGAAGATCC 
ACAGAGCTCA TCCCACGGTT AAAGGGAGTA CTAACCAATC CAAATTGTGA ATTTGAAGCT 
AACTACGTTG CCATACACAC CCTTGCCACT TGGTACAAGT CAAACATGAA CGGAGTGAGG 
AAGGATGTGG TGATGACTGA CAGTGAAGAC AGTACTGTAT CAATACAGAT AAAGTTAGAA 
AATGAAGGCA GTGACGAAGA CATTGAGACT GATGTTCTCT ACAGCCCACA GATGGCTTTA 
AAGCTGGCTT TAACTGAGTG GCTGCAGGAG TTCGGTGTCC CTCATCAGTA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAGACAAGT GTAGTTGATG GGACTCCCTT AGTTGCTGCA 
CCCTCTTTAA ATGCCACAAC TGTAGTTACA ACAGTTTACC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCTT TGAGTGGTGA ACCCTCAGTT CTGACCAAGG AAGAGGAAAA GAAACAGCCA 
GATGAGGAAC CCATGGATAT GGTGGTCGAA AAGCAAGAAG AAGCAGAGCA CAAGAATGAC 
AACCAGATAC TAACTGAGAT TGTTGAAGCG AAGATGGCAG AGGAGTTGAG ACCAATGGAC 
ACGGATAAGG AGAGCATGGC TGAGTCCAAA TCCCCAGAGA TGTCCATGCA GGAAGACTGC 
ATTCCTGACG TGGCTCCTAT GCAGACAGAT GAGCAAACAC AGAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTCT GTACACCGCA GAGCCTGTGA CTCTGGAGGA CTTACAGTTA 
CTCGCTGACC TGTTCTACCT TCCTTACGAG CACGGGCCCA AAGGAGCGCA GATGCTACGG 
GAATTCCAGT GGCTTCGAGC AAACAGCAGT GTTGTCAGTG TCAACTGCAA AGGGAAAGAC 
TCAGAAAAAA TAGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGCACTA 
GTGATGGGGA TGTTCACCCG GCTTTCCAAC TGTGCCAACA GGACCATCCT TTATGACATG 
TACTCCTATG TGTGGGACAT CAAGAGTATA ATGTCTATGG TGAAATCTTT TGTGCAGTGG 
TTAGGGTGTC GTAGTCACTC TTCAGCACAG TTCTTAATTG GAGACCAGGA GCCCTGGGCC 
TTTAGAGGTG GTCTAGCAGG AGAGTTCCAG CGTTTGCTGC CAATTGATGG AGCAAATGAT 
CTCTTTTTTC AACCACCCCC ACTGACTCCA ACCTCCAAAG TTTATACTAT CAGACCTTAT 
TTCCCTAAAG ATGAGGCTTC CGTGTACAAG ATTTGCAGAG AAATGTATGA TGATGGAGTG 
GGTTTCCCTT TTCAAAGTCA ACCTGACCTT ATTGGAGACA AGTTAGTAGG AGGACTGCTC 
TCCCTCAGCC TGGATTACTG CTTCGTCCTA GAAGATGAAG ATGGCATTTG TGGTTATGCC 
TTAGGCACAG TAGATGTGAC CCCCTTCATT AAAAAGTGTA AAATTTCCTG GATTCCCTTC 
ATGCAAGAGA AGTATACCAA GCCAAATGGT GACAAAGAGC TCTCAGAGGC TGAGAAAATA 
ATGTTGAGTT TCCATGAAGA GCAGGAGGTG TTGCCAGAAA CTTTCCTTGC CAACTTCCCT 
TCTCTGATAA AAATGGATAT TCATAAAAAA GTGACTGACC CGAGTGTTGC CAAAAGCATG 
ATGGCTTGCC TCCTGTCTTC ACTGAAGGCC AATGGCTCCC GGGGGGCTTT CTGTGAAGTA 
AGACCAGATG ATAAAAGGAT TCTGGAATTT TACAGCAAGT TAGGCTGTTT TGAAATTGCA 
AAAATGGAAG GATTTCCAAA GGATGTGGTT ATACTTGGTC GAAGCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Oga ( XM_017318305.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173794.1 Mus musculus meningioma expressed antigen 5 (hyaluronidase) (Mgea5), transcript variant X1, mRNA.

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CloneID	OMu80024
Clone ID Related Accession (Same CDS sequence)	XM_017318305.1 , XM_017318305.2
Accession Version	XM_017318305.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein O-GlcNAcase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGGAGAGCCA GGCGGCGCTG GAGGAGCGGG AGAGCGAGCG CAACGCCAAC 
CCTGCCGCCG CCTCGGGGGC TTCGTTGGAG CAGTCGGTAG CTCCCGCCCC CGGAGAAGAT 
AACCCATCGG GAGCCGGGGC TGCGGCCGTG GTCGGGGCGG CCGGAGGGGC CCGCAGGTTC 
CTGTGCGGTG TCGTGGAAGG GTTTTATGGA AGACCTTGGG TTATGGAGCA GAGAAAAGAG 
CTCTTTAGAA GGCTTCAGAA ATGGGAATTA AACACATACT TGTATGCTCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AAGAAGCTGA GCAACTTATG 
ACTCTGATCT CTGCTGCACG AGAATATGAA ATAGAATTCA TCTATGCTAT TTCGCCAGGA 
CTAGATATCA CTTTTTCTAA CCCCAAGGAA GTATCAACGT TGAAACGCAA ATTGGACCAG 
GTCTCTCAGT TTGGGTGCAG GTCGTTTGCT TTGCTTTTTG ATGATATTGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT GTTCAGTTCT TTTGCACATG CACAAGTTTC CATCACCAAT 
GAAATTTATC AGTACCTGGG AGAGCCAGAA ACCTTCCTCT TCTGCCCTAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTATCTCAG TCTCCTTACT TGAGGACTGT GGGTGAGAAG 
CTTCTACCTG GAATTGAGGT GCTTTGGACA GGTCCAAAAG TTGTTTCAAA AGAAATTCCA 
GTGGAATCTA TTGAAGAAGT TTCTAAGATC ATTAAGAGAG CTCCAGTGAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGG CTGTTTCTGG GTCCCTACAA AGGAAGATCC 
ACAGAGCTCA TCCCACGGTT AAAGGGAGTA CTAACCAATC CAAATTGTGA ATTTGAAGCT 
AACTACGTTG CCATACACAC CCTTGCCACT TGGTACAAGT CAAACATGAA CGGAGTGAGG 
AAGGATGTGG TGATGACTGA CAGTGAAGAC AGTACTGTAT CAATACAGAT AAAGTTAGAA 
AATGAAGGCA GTGACGAAGA CATTGAGACT GATGTTCTCT ACAGCCCACA GATGGCTTTA 
AAGCTGGCTT TAACTGAGTG GCTGCAGGAG TTCGGTGTCC CTCATCAGTA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAGACAAGT GTAGTTGATG GGACTCCCTT AGTTGCTGCA 
CCCTCTTTAA ATGCCACAAC TGTAGTTACA ACAGTTTACC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCTT TGAGTGGTGA ACCCTCAGTT CTGACCAAGG AAGAGGAAAA GAAACAGCCA 
GATGAGGAAC CCATGGATAT GGTGGTCGAA AAGCAAGAAG AAGCAGAGCA CAAGAATGAC 
AACCAGATAC TAACTGAGAT TGTTGAAGCG AAGATGGCAG AGGAGTTGAG ACCAATGGAC 
ACGGATAAGG AGAGCATGGC TGAGTCCAAA TCCCCAGAGA TGTCCATGCA GGAAGACTGC 
ATTCCTGACG TGGCTCCTAT GCAGACAGAT GAGCAAACAC AGAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTCT GTACACCGCA GAGCCTGTGA CTCTGGAGGA CTTACAGTTA 
CTCGCTGACC TGTTCTACCT TCCTTACGAG CACGGGCCCA AAGGAGCGCA GATGCTACGG 
GAATTCCAGT GGCTTCGAGC AAACAGCAGT GTTGTCAGTG TCAACTGCAA AGGGAAAGAC 
TCAGAAAAAA TAGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGCACTA 
GTGATGGGGA TGTTCACCCG GCTTTCCAAC TGTGCCAACA GGACCATCCT TTATGACATG 
TACTCCTATG TGTGGGACAT CAAGAGTATA ATGTCTATGG TGAAATCTTT TGTGCAGTGG 
TTAGATGGGA GAATCCTCAG CACAAGTGTC TACTGCTATT GGATGGACAG CGGCAGATGT 
TCACAGCGCG TCATGATTAA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173794.1
CDS	424..2487
Translation	MVQKESQAALEERESERNANPAAASGASLEQSVAPAPGEDNPSGAGAAAVVGAAGGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKTSVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPSVLTKEEEKKQPDEEPMDMVVEKQEEAEHKNDNQILTEIVEAKMAEELRPMDTDKESMAESKSPEMSMQEDCIPDVAPMQTDEQTQKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCALVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLDGRILSTSVYCYWMDSGRCSQRVMIN

Target ORF information:

RefSeq Version	XM_017318305.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus meningioma expressed antigen 5 (hyaluronidase) (Mgea5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318305.1

ORF Insert Sequence:

ATGGTGCAGA AGGAGAGCCA GGCGGCGCTG GAGGAGCGGG AGAGCGAGCG CAACGCCAAC 
CCTGCCGCCG CCTCGGGGGC TTCGTTGGAG CAGTCGGTAG CTCCCGCCCC CGGAGAAGAT 
AACCCATCGG GAGCCGGGGC TGCGGCCGTG GTCGGGGCGG CCGGAGGGGC CCGCAGGTTC 
CTGTGCGGTG TCGTGGAAGG GTTTTATGGA AGACCTTGGG TTATGGAGCA GAGAAAAGAG 
CTCTTTAGAA GGCTTCAGAA ATGGGAATTA AACACATACT TGTATGCTCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AAGAAGCTGA GCAACTTATG 
ACTCTGATCT CTGCTGCACG AGAATATGAA ATAGAATTCA TCTATGCTAT TTCGCCAGGA 
CTAGATATCA CTTTTTCTAA CCCCAAGGAA GTATCAACGT TGAAACGCAA ATTGGACCAG 
GTCTCTCAGT TTGGGTGCAG GTCGTTTGCT TTGCTTTTTG ATGATATTGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT GTTCAGTTCT TTTGCACATG CACAAGTTTC CATCACCAAT 
GAAATTTATC AGTACCTGGG AGAGCCAGAA ACCTTCCTCT TCTGCCCTAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTATCTCAG TCTCCTTACT TGAGGACTGT GGGTGAGAAG 
CTTCTACCTG GAATTGAGGT GCTTTGGACA GGTCCAAAAG TTGTTTCAAA AGAAATTCCA 
GTGGAATCTA TTGAAGAAGT TTCTAAGATC ATTAAGAGAG CTCCAGTGAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGG CTGTTTCTGG GTCCCTACAA AGGAAGATCC 
ACAGAGCTCA TCCCACGGTT AAAGGGAGTA CTAACCAATC CAAATTGTGA ATTTGAAGCT 
AACTACGTTG CCATACACAC CCTTGCCACT TGGTACAAGT CAAACATGAA CGGAGTGAGG 
AAGGATGTGG TGATGACTGA CAGTGAAGAC AGTACTGTAT CAATACAGAT AAAGTTAGAA 
AATGAAGGCA GTGACGAAGA CATTGAGACT GATGTTCTCT ACAGCCCACA GATGGCTTTA 
AAGCTGGCTT TAACTGAGTG GCTGCAGGAG TTCGGTGTCC CTCATCAGTA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAGACAAGT GTAGTTGATG GGACTCCCTT AGTTGCTGCA 
CCCTCTTTAA ATGCCACAAC TGTAGTTACA ACAGTTTACC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCTT TGAGTGGTGA ACCCTCAGTT CTGACCAAGG AAGAGGAAAA GAAACAGCCA 
GATGAGGAAC CCATGGATAT GGTGGTCGAA AAGCAAGAAG AAGCAGAGCA CAAGAATGAC 
AACCAGATAC TAACTGAGAT TGTTGAAGCG AAGATGGCAG AGGAGTTGAG ACCAATGGAC 
ACGGATAAGG AGAGCATGGC TGAGTCCAAA TCCCCAGAGA TGTCCATGCA GGAAGACTGC 
ATTCCTGACG TGGCTCCTAT GCAGACAGAT GAGCAAACAC AGAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTCT GTACACCGCA GAGCCTGTGA CTCTGGAGGA CTTACAGTTA 
CTCGCTGACC TGTTCTACCT TCCTTACGAG CACGGGCCCA AAGGAGCGCA GATGCTACGG 
GAATTCCAGT GGCTTCGAGC AAACAGCAGT GTTGTCAGTG TCAACTGCAA AGGGAAAGAC 
TCAGAAAAAA TAGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGCACTA 
GTGATGGGGA TGTTCACCCG GCTTTCCAAC TGTGCCAACA GGACCATCCT TTATGACATG 
TACTCCTATG TGTGGGACAT CAAGAGTATA ATGTCTATGG TGAAATCTTT TGTGCAGTGG 
TTAGATGGGA GAATCCTCAG CACAAGTGTC TACTGCTATT GGATGGACAG CGGCAGATGT 
TCACAGCGCG TCATGATTAA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Oga ( XM_017318305.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173794.1 Mus musculus O-GlcNAcase (Oga), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80024
Clone ID Related Accession (Same CDS sequence)	XM_017318305.1 , XM_017318305.2
Accession Version	XM_017318305.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein O-GlcNAcase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318305.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGGAGAGCCA GGCGGCGCTG GAGGAGCGGG AGAGCGAGCG CAACGCCAAC 
CCTGCCGCCG CCTCGGGGGC TTCGTTGGAG CAGTCGGTAG CTCCCGCCCC CGGAGAAGAT 
AACCCATCGG GAGCCGGGGC TGCGGCCGTG GTCGGGGCGG CCGGAGGGGC CCGCAGGTTC 
CTGTGCGGTG TCGTGGAAGG GTTTTATGGA AGACCTTGGG TTATGGAGCA GAGAAAAGAG 
CTCTTTAGAA GGCTTCAGAA ATGGGAATTA AACACATACT TGTATGCTCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AAGAAGCTGA GCAACTTATG 
ACTCTGATCT CTGCTGCACG AGAATATGAA ATAGAATTCA TCTATGCTAT TTCGCCAGGA 
CTAGATATCA CTTTTTCTAA CCCCAAGGAA GTATCAACGT TGAAACGCAA ATTGGACCAG 
GTCTCTCAGT TTGGGTGCAG GTCGTTTGCT TTGCTTTTTG ATGATATTGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT GTTCAGTTCT TTTGCACATG CACAAGTTTC CATCACCAAT 
GAAATTTATC AGTACCTGGG AGAGCCAGAA ACCTTCCTCT TCTGCCCTAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTATCTCAG TCTCCTTACT TGAGGACTGT GGGTGAGAAG 
CTTCTACCTG GAATTGAGGT GCTTTGGACA GGTCCAAAAG TTGTTTCAAA AGAAATTCCA 
GTGGAATCTA TTGAAGAAGT TTCTAAGATC ATTAAGAGAG CTCCAGTGAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGG CTGTTTCTGG GTCCCTACAA AGGAAGATCC 
ACAGAGCTCA TCCCACGGTT AAAGGGAGTA CTAACCAATC CAAATTGTGA ATTTGAAGCT 
AACTACGTTG CCATACACAC CCTTGCCACT TGGTACAAGT CAAACATGAA CGGAGTGAGG 
AAGGATGTGG TGATGACTGA CAGTGAAGAC AGTACTGTAT CAATACAGAT AAAGTTAGAA 
AATGAAGGCA GTGACGAAGA CATTGAGACT GATGTTCTCT ACAGCCCACA GATGGCTTTA 
AAGCTGGCTT TAACTGAGTG GCTGCAGGAG TTCGGTGTCC CTCATCAGTA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAGACAAGT GTAGTTGATG GGACTCCCTT AGTTGCTGCA 
CCCTCTTTAA ATGCCACAAC TGTAGTTACA ACAGTTTACC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCTT TGAGTGGTGA ACCCTCAGTT CTGACCAAGG AAGAGGAAAA GAAACAGCCA 
GATGAGGAAC CCATGGATAT GGTGGTCGAA AAGCAAGAAG AAGCAGAGCA CAAGAATGAC 
AACCAGATAC TAACTGAGAT TGTTGAAGCG AAGATGGCAG AGGAGTTGAG ACCAATGGAC 
ACGGATAAGG AGAGCATGGC TGAGTCCAAA TCCCCAGAGA TGTCCATGCA GGAAGACTGC 
ATTCCTGACG TGGCTCCTAT GCAGACAGAT GAGCAAACAC AGAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTCT GTACACCGCA GAGCCTGTGA CTCTGGAGGA CTTACAGTTA 
CTCGCTGACC TGTTCTACCT TCCTTACGAG CACGGGCCCA AAGGAGCGCA GATGCTACGG 
GAATTCCAGT GGCTTCGAGC AAACAGCAGT GTTGTCAGTG TCAACTGCAA AGGGAAAGAC 
TCAGAAAAAA TAGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGCACTA 
GTGATGGGGA TGTTCACCCG GCTTTCCAAC TGTGCCAACA GGACCATCCT TTATGACATG 
TACTCCTATG TGTGGGACAT CAAGAGTATA ATGTCTATGG TGAAATCTTT TGTGCAGTGG 
TTAGATGGGA GAATCCTCAG CACAAGTGTC TACTGCTATT GGATGGACAG CGGCAGATGT 
TCACAGCGCG TCATGATTAA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173794.1
CDS	515..2578
Translation	MVQKESQAALEERESERNANPAAASGASLEQSVAPAPGEDNPSGAGAAAVVGAAGGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKTSVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPSVLTKEEEKKQPDEEPMDMVVEKQEEAEHKNDNQILTEIVEAKMAEELRPMDTDKESMAESKSPEMSMQEDCIPDVAPMQTDEQTQKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCALVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLDGRILSTSVYCYWMDSGRCSQRVMIN

Target ORF information:

RefSeq Version	XM_017318305.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus O-GlcNAcase (Oga), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318305.2

ORF Insert Sequence:

ATGGTGCAGA AGGAGAGCCA GGCGGCGCTG GAGGAGCGGG AGAGCGAGCG CAACGCCAAC 
CCTGCCGCCG CCTCGGGGGC TTCGTTGGAG CAGTCGGTAG CTCCCGCCCC CGGAGAAGAT 
AACCCATCGG GAGCCGGGGC TGCGGCCGTG GTCGGGGCGG CCGGAGGGGC CCGCAGGTTC 
CTGTGCGGTG TCGTGGAAGG GTTTTATGGA AGACCTTGGG TTATGGAGCA GAGAAAAGAG 
CTCTTTAGAA GGCTTCAGAA ATGGGAATTA AACACATACT TGTATGCTCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AAGAAGCTGA GCAACTTATG 
ACTCTGATCT CTGCTGCACG AGAATATGAA ATAGAATTCA TCTATGCTAT TTCGCCAGGA 
CTAGATATCA CTTTTTCTAA CCCCAAGGAA GTATCAACGT TGAAACGCAA ATTGGACCAG 
GTCTCTCAGT TTGGGTGCAG GTCGTTTGCT TTGCTTTTTG ATGATATTGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT GTTCAGTTCT TTTGCACATG CACAAGTTTC CATCACCAAT 
GAAATTTATC AGTACCTGGG AGAGCCAGAA ACCTTCCTCT TCTGCCCTAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTATCTCAG TCTCCTTACT TGAGGACTGT GGGTGAGAAG 
CTTCTACCTG GAATTGAGGT GCTTTGGACA GGTCCAAAAG TTGTTTCAAA AGAAATTCCA 
GTGGAATCTA TTGAAGAAGT TTCTAAGATC ATTAAGAGAG CTCCAGTGAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGG CTGTTTCTGG GTCCCTACAA AGGAAGATCC 
ACAGAGCTCA TCCCACGGTT AAAGGGAGTA CTAACCAATC CAAATTGTGA ATTTGAAGCT 
AACTACGTTG CCATACACAC CCTTGCCACT TGGTACAAGT CAAACATGAA CGGAGTGAGG 
AAGGATGTGG TGATGACTGA CAGTGAAGAC AGTACTGTAT CAATACAGAT AAAGTTAGAA 
AATGAAGGCA GTGACGAAGA CATTGAGACT GATGTTCTCT ACAGCCCACA GATGGCTTTA 
AAGCTGGCTT TAACTGAGTG GCTGCAGGAG TTCGGTGTCC CTCATCAGTA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAGACAAGT GTAGTTGATG GGACTCCCTT AGTTGCTGCA 
CCCTCTTTAA ATGCCACAAC TGTAGTTACA ACAGTTTACC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCTT TGAGTGGTGA ACCCTCAGTT CTGACCAAGG AAGAGGAAAA GAAACAGCCA 
GATGAGGAAC CCATGGATAT GGTGGTCGAA AAGCAAGAAG AAGCAGAGCA CAAGAATGAC 
AACCAGATAC TAACTGAGAT TGTTGAAGCG AAGATGGCAG AGGAGTTGAG ACCAATGGAC 
ACGGATAAGG AGAGCATGGC TGAGTCCAAA TCCCCAGAGA TGTCCATGCA GGAAGACTGC 
ATTCCTGACG TGGCTCCTAT GCAGACAGAT GAGCAAACAC AGAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTCT GTACACCGCA GAGCCTGTGA CTCTGGAGGA CTTACAGTTA 
CTCGCTGACC TGTTCTACCT TCCTTACGAG CACGGGCCCA AAGGAGCGCA GATGCTACGG 
GAATTCCAGT GGCTTCGAGC AAACAGCAGT GTTGTCAGTG TCAACTGCAA AGGGAAAGAC 
TCAGAAAAAA TAGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGCACTA 
GTGATGGGGA TGTTCACCCG GCTTTCCAAC TGTGCCAACA GGACCATCCT TTATGACATG 
TACTCCTATG TGTGGGACAT CAAGAGTATA ATGTCTATGG TGAAATCTTT TGTGCAGTGG 
TTAGATGGGA GAATCCTCAG CACAAGTGTC TACTGCTATT GGATGGACAG CGGCAGATGT 
TCACAGCGCG TCATGATTAA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

O-GlcNAc is required for the survival of primed pluripotent stem cells and their reversion to the na?ve state.
Biochemical and biophysical research communications480(4)655-661(2016 11)
Miura T,Nishihara S

O-Linked N-Acetylglucosamine (O-GlcNAc) Transferase and O-GlcNAcase Interact with Mi2? Protein at the A?-Globin Promoter.
The Journal of biological chemistry291(30)15628-40(2016 07)
Zhang Z,Costa FC,Tan EP,Bushue N,DiTacchio L,Costello CE,McComb ME,Whelan SA,Peterson KR,Slawson C

Obesity resistance and increased energy expenditure by white adipose tissue browning in Oga(+/-) mice.
Diabetologia58(12)2867-76(2015 Dec)
Yang YR,Jang HJ,Choi SS,Lee YH,Lee GH,Seo YK,Choi JH,Park D,Koh A,Kim IS,Lee H,Ryu SH,Suh PG

E2F1 Transcription Factor Regulates O-linked N-acetylglucosamine (O-GlcNAc) Transferase and O-GlcNAcase Expression.
The Journal of biological chemistry290(52)31013-24(2015 Dec)
Muthusamy S,Hong KU,Dassanayaka S,Hamid T,Jones SP

O-GlcNAc cycling enzymes control vascular development of the placenta by modulating the levels of HIF-1?.
Placenta36(10)1063-8(2015 Oct)
Yang YR,Jang HJ,Lee YH,Kim IS,Lee H,Ryu SH,Suh PG

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Oga cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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