OGA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	OGA
Entrez Gene ID	10724
Full Name	meningioma expressed antigen 5 (hyaluronidase)
Synonyms	MEA5,NCOAT,OGA
General protein information	Preferred Names meningioma expressed antigen 5 (hyaluronidase) Names protein O-GlcNAcase N-acetyl-beta-D-glucosaminidase N-acetyl-beta-glucosaminidase O-GlcNAcase beta-N-acetylglucosaminidase beta-N-acetylhexosaminidase beta-hexosaminidase bifunctional protein NCOAT hyaluronidase in meningioma meningioma-expressed antigen 5 nuclear cytoplasmic O-GlcNAcase and acetyltransferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q24.32
Summary	The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008].
Disorder MIM:	604039

mRNA and Protein(s)

mRNA	Protein	Name
NM_012215.3	NP_036347.1	protein O-GlcNAcase isoform a
NM_012215.4	NP_036347.1	protein O-GlcNAcase isoform a
XM_024447782.1	XP_024303550.1	protein O-GlcNAcase isoform X3
XM_017015585.1	XP_016871074.1	protein O-GlcNAcase isoform X1
NM_012215.5	NP_036347.1	protein O-GlcNAcase isoform a
XM_017015586.1	XP_016871075.1	protein O-GlcNAcase isoform X2
NM_001142434.1	NP_001135906.1	protein O-GlcNAcase isoform b

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mgea5	NP_076288.1
Bos taurus (cattle)	MGEA5	NP_001193377.1
Danio rerio (zebrafish)	mgea5	XP_002664185.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002287	XP_307891.5
Caenorhabditis elegans (roundworm)	oga-1	NP_001123184.1
Xenopus tropicalis (tropical clawed frog)	mgea5	NP_001120363.1
Rattus norvegicus (Norway rat)	Mgea5	NP_571979.1
Pan troglodytes (chimpanzee)	MGEA5	XP_507996.3
Canis lupus familiaris (dog)	MGEA5	XP_534996.2
Gallus gallus (chicken)	MGEA5	NP_001034394.1
Homo sapiens (human)	MGEA5	NP_036347.1
Drosophila melanogaster (fruit fly)	Oga	NP_650956.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural insights into the substrate binding adaptability and specificity of human O-GlcNAcase.
Li B, Li H, Hu CW, Jiang J
Nature communications8(1)666(2017 09)

Hijacking of the O-GlcNAcZYME complex by the HTLV-1 Tax oncoprotein facilitates viral transcription.
Groussaud D, Khair M, Tollenaere AI, Waast L, Kuo MS, Mangeney M, Martella C, Fardini Y, Coste S, Souidi M, Benit L, Pique C, Issad T
PLoS pathogens13(7)e1006518(2017 Jul)

The Sulfur-Linked Analogue of O-GlcNAc (S-GlcNAc) Is an Enzymatically Stable and Reasonable Structural Surrogate for O-GlcNAc at the Peptide and Protein Levels.
De Leon CA, Levine PM, Craven TW, Pratt MR
Biochemistry56(27)3507-3517(2017 07)

Structures of human O-GlcNAcase and its complexes reveal a new substrate recognition mode.
Li B, Li H, Lu L, Jiang J
Nature structural & molecular biology24(4)362-369(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tax interacts with the host OGT/OGA complex and inhibits the activity of OGT-bound OGA.
Title: Hijacking of the O-GlcNAcZYME complex by the HTLV-1 Tax oncoprotein facilitates viral transcription.

Data suggest that the substrate specificity of O-GlcNAcase/OGA does not extend to proteins/peptides modified with S-GlcNAc (an analog of O-GlcNAc); proteins modified with S-GlcNAc appear to be stable against O-GlcNAcase/OGA hydrolysis.
Title: The Sulfur-Linked Analogue of O-GlcNAc (S-GlcNAc) Is an Enzymatically Stable and Reasonable Structural Surrogate for O-GlcNAc at the Peptide and Protein Levels.

hOGA forms an unusual arm-in-arm homodimer in which the catalytic domain of one monomer is covered by the stalk domain of the sister monomer to create a substrate-binding cleft.
Title: Structures of human O-GlcNAcase and its complexes reveal a new substrate recognition mode.

OGA is physically associated with the known RNA polymerase II (pol II) pausing/elongation factors SPT5 and TRIM28-KAP1-TIF1beta, and a purified OGA-SPT5-TIF1beta complex has elongation properties.
Title: O-GlcNAcase Is an RNA Polymerase II Elongation Factor Coupled to Pausing Factors SPT5 and TIF1?.

the O-linked N-acetylglucosamine (O-GlcNAc) processing enzymes, O-GlcNAc-transferase (OGT) and O-GlcNAcase (OGA), interact with the (A)gamma-globin promoter at the -566 GATA repressor site
Title: O-Linked N-Acetylglucosamine (O-GlcNAc) Transferase and O-GlcNAcase Interact with Mi2? Protein at the A?-Globin Promoter.

The following OGA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OGA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06111	NM_012215.5 Latest version!	Homo sapiens O-GlcNAcase (OGA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu07523	NM_001142434.1 Latest version!	Homo sapiens O-GlcNAcase (OGA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$615.30 $879.00
OHu64331	XM_017015585.1 Latest version!	Homo sapiens O-GlcNAcase (OGA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu106417	XM_024447782.1 Latest version!	Homo sapiens O-GlcNAcase (OGA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu91033	XM_017015586.1 Latest version!	Homo sapiens O-GlcNAcase (OGA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu06111	NM_012215.3	Homo sapiens meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu06111	NM_012215.4	Homo sapiens O-GlcNAcase (OGA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OGA ( NM_012215.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036347.1 Homo sapiens meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06111
Clone ID Related Accession (Same CDS sequence)	NM_012215.3 , NM_012215.4 , NM_012215.5
Accession Version	NM_012215.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	protein O-GlcNAcase isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC353958.1, BC047877.1, AB014579.2 and BQ016159.1. On Dec 3, 2008 this sequence version replaced NM_012215.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF036144.2, SRR1803616.4396.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGTGCAGA AGGAGAGTCA AGCGACGTTG GAGGAGCGGG AGAGCGAGCT CAGCTCCAAC 
CCTGCCGCCT CTGCGGGGGC ATCGCTGGAG CCGCCGGCAG CTCCGGCACC CGGAGAAGAC 
AACCCCGCCG GGGCTGGGGG AGCGGCGGTG GCCGGGGCTG CAGGAGGGGC TCGGCGGTTC 
CTCTGCGGTG TGGTGGAAGG ATTTTATGGA AGACCTTGGG TTATGGAACA GAGAAAAGAA 
CTCTTTAGAA GGCTCCAGAA ATGGGAATTA AATACATACT TGTATGCCCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AGGAAGCTGA GCAACTTATG 
ACTCTCATCT CTGCTGCACG AGAATATGAG ATAGAGTTCA TCTATGCGAT CTCACCTGGA 
TTGGATATCA CTTTTTCTAA CCCCAAGGAA GTATCCACAT TGAAACGTAA ATTGGACCAG 
GTTTCTCAGT TTGGGTGCAG ATCATTTGCT TTGCTTTTTG ATGATATAGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT ATTCAGTTCT TTTGCTCATG CCCAAGTCTC CATCACAAAT 
GAAATCTATC AGTACCTAGG AGAGCCAGAA ACTTTCCTCT TCTGTCCCAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTGTCTCAG TCTCCATATT TAAGGACTGT GGGTGAAAAG 
CTTCTACCTG GAATTGAAGT GCTTTGGACA GGTCCCAAAG TTGTTTCTAA AGAAATTCCA 
GTAGAGTCCA TCGAAGAGGT TTCTAAGATT ATTAAGAGAG CTCCAGTAAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGA CTGTTTCTGG GCCCGTACAA AGGAAGATCC 
ACAGAACTCA TCCCACGGTT AAAAGGAGTC CTCACTAATC CAAATTGTGA ATTTGAAGCC 
AACTACGTTG CTATCCACAC CCTTGCCACC TGGTACAAAT CAAACATGAA TGGAGTGAGA 
AAAGATGTAG TGATGACTGA CAGTGAAGAT AGTACTGTGT CCATCCAGAT AAAATTAGAA 
AATGAAGGCA GTGATGAAGA TATTGAAACT GATGTACTCT ATAGTCCACA GATGGCTCTA 
AAGCTAGCAT TAACAGAATG GTTGCAAGAG TTTGGTGTGC CTCATCAATA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAAGCAAGT GTAGTTGATG GGACTCCTTT AGTTGCAGCA 
CCCTCTTTAA ATGCCACAAC CGTAGTAACA ACAGTTTATC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCCT TGAGTGGTGA GCCTACTACT CTGACCAAGG AAGAAGAAAA GAAACAGCCT 
GATGAAGAAC CCATGGACAT GGTGGTGGAA AAACAAGAAG AAACGGACCA CAAGAATGAC 
AATCAAATAC TGAGTGAAAT TGTTGAAGCG AAAATGGCAG AGGAATTGAA ACCAATGGAC 
ACTGATAAAG AGAGCATAGC TGAATCAAAA TCCCCAGAGA TGTCCATGCA AGAAGATTGT 
ATTAGTGACA TTGCCCCCAT GCAAACTGAT GAACAGACAA ACAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTTT GTACACTGCG GAACCAGTGA CCCTGGAGGA TTTGCAGTTA 
CTTGCTGATC TATTCTACCT TCCTTACGAG CATGGACCCA AAGGAGCACA GATGTTACGG 
GAATTTCAAT GGCTTCGAGC AAATAGTAGT GTTGTCAGTG TCAATTGCAA AGGAAAAGAC 
TCTGAAAAAA TTGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGGACTA 
GTGATGGGAA TGTTCACTCG GCTCTCCAAT TGTGCCAACA GGACAATTCT TTATGACATG 
TACTCCTATG TTTGGGATAT CAAGAGTATA ATGTCTATGG TGAAGTCTTT TGTACAGTGG 
TTAGGGTGTC GTAGTCATTC TTCAGCACAA TTCTTAATTG GAGACCAAGA ACCCTGGGCC 
TTTAGAGGTG GTCTAGCAGG AGAGTTCCAG CGTTTGCTGC CAATTGATGG GGCAAATGAT 
CTCTTTTTTC AGCCACCTCC ACTGACTCCT ACCTCCAAAG TTTATACTAT CAGACCTTAT 
TTTCCTAAGG ATGAGGCATC CGTGTACAAG ATTTGCAGAG AAATGTATGA CGATGGAGTG 
GGTTTACCCT TTCAAAGTCA GCCTGATCTT ATTGGAGACA AGTTAGTAGG AGGGCTGCTT 
TCCCTCAGCC TGGATTACTG CTTTGTCCTA GAAGATGAAG ATGGCATATG TGGTTATGCC 
TTGGGCACTG TAGATGTGAC CCCCTTTATT AAAAAATGTA AAATTTCCTG GATCCCCTTC 
ATGCAGGAGA AGTATACCAA GCCAAATGGT GACAAGGAAC TCTCTGAGGC TGAGAAAATA 
ATGTTGAGTT TCCATGAAGA ACAGGAAGTA CTGCCAGAAA CTTTCCTTGC TAATTTCCCT 
TCTCTGATAA AGATGGACAT TCACAAAAAA GTAACTGACC CAAGTGTGGC CAAAAGCATG 
ATGGCTTGCC TCCTGTCTTC ACTGAAGGCT AATGGCTCCC GGGGAGCTTT CTGTGAAGTG 
AGACCAGATG ATAAAAGAAT TCTGGAATTT TACAGCAAGT TAGGATGTTT TGAAATTGCA 
AAAATGGAAG GATTTCCAAA GGATGTGGTT ATACTTGGTC GGAGCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036347.1
CDS	444..3194
Translation	MVQKESQATLEERESELSSNPAASAGASLEPPAAPAPGEDNPAGAGGAAVAGAAGGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKASVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPTTLTKEEEKKQPDEEPMDMVVEKQEETDHKNDNQILSEIVEAKMAEELKPMDTDKESIAESKSPEMSMQEDCISDIAPMQTDEQTNKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCGLVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGANDLFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGLPFQSQPDLIGDKLVGGLLSLSLDYCFVLEDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGRSL

Target ORF information:

RefSeq Version	NM_012215.3
Organism	Homo sapiens(human)
Definition	Homo sapiens meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012215.3

ORF Insert Sequence:

ATGGTGCAGA AGGAGAGTCA AGCGACGTTG GAGGAGCGGG AGAGCGAGCT CAGCTCCAAC 
CCTGCCGCCT CTGCGGGGGC ATCGCTGGAG CCGCCGGCAG CTCCGGCACC CGGAGAAGAC 
AACCCCGCCG GGGCTGGGGG AGCGGCGGTG GCCGGGGCTG CAGGAGGGGC TCGGCGGTTC 
CTCTGCGGTG TGGTGGAAGG ATTTTATGGA AGACCTTGGG TTATGGAACA GAGAAAAGAA 
CTCTTTAGAA GGCTCCAGAA ATGGGAATTA AATACATACT TGTATGCCCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AGGAAGCTGA GCAACTTATG 
ACTCTCATCT CTGCTGCACG AGAATATGAG ATAGAGTTCA TCTATGCGAT CTCACCTGGA 
TTGGATATCA CTTTTTCTAA CCCCAAGGAA GTATCCACAT TGAAACGTAA ATTGGACCAG 
GTTTCTCAGT TTGGGTGCAG ATCATTTGCT TTGCTTTTTG ATGATATAGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT ATTCAGTTCT TTTGCTCATG CCCAAGTCTC CATCACAAAT 
GAAATCTATC AGTACCTAGG AGAGCCAGAA ACTTTCCTCT TCTGTCCCAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTGTCTCAG TCTCCATATT TAAGGACTGT GGGTGAAAAG 
CTTCTACCTG GAATTGAAGT GCTTTGGACA GGTCCCAAAG TTGTTTCTAA AGAAATTCCA 
GTAGAGTCCA TCGAAGAGGT TTCTAAGATT ATTAAGAGAG CTCCAGTAAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGA CTGTTTCTGG GCCCGTACAA AGGAAGATCC 
ACAGAACTCA TCCCACGGTT AAAAGGAGTC CTCACTAATC CAAATTGTGA ATTTGAAGCC 
AACTACGTTG CTATCCACAC CCTTGCCACC TGGTACAAAT CAAACATGAA TGGAGTGAGA 
AAAGATGTAG TGATGACTGA CAGTGAAGAT AGTACTGTGT CCATCCAGAT AAAATTAGAA 
AATGAAGGCA GTGATGAAGA TATTGAAACT GATGTACTCT ATAGTCCACA GATGGCTCTA 
AAGCTAGCAT TAACAGAATG GTTGCAAGAG TTTGGTGTGC CTCATCAATA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAAGCAAGT GTAGTTGATG GGACTCCTTT AGTTGCAGCA 
CCCTCTTTAA ATGCCACAAC CGTAGTAACA ACAGTTTATC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCCT TGAGTGGTGA GCCTACTACT CTGACCAAGG AAGAAGAAAA GAAACAGCCT 
GATGAAGAAC CCATGGACAT GGTGGTGGAA AAACAAGAAG AAACGGACCA CAAGAATGAC 
AATCAAATAC TGAGTGAAAT TGTTGAAGCG AAAATGGCAG AGGAATTGAA ACCAATGGAC 
ACTGATAAAG AGAGCATAGC TGAATCAAAA TCCCCAGAGA TGTCCATGCA AGAAGATTGT 
ATTAGTGACA TTGCCCCCAT GCAAACTGAT GAACAGACAA ACAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTTT GTACACTGCG GAACCAGTGA CCCTGGAGGA TTTGCAGTTA 
CTTGCTGATC TATTCTACCT TCCTTACGAG CATGGACCCA AAGGAGCACA GATGTTACGG 
GAATTTCAAT GGCTTCGAGC AAATAGTAGT GTTGTCAGTG TCAATTGCAA AGGAAAAGAC 
TCTGAAAAAA TTGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGGACTA 
GTGATGGGAA TGTTCACTCG GCTCTCCAAT TGTGCCAACA GGACAATTCT TTATGACATG 
TACTCCTATG TTTGGGATAT CAAGAGTATA ATGTCTATGG TGAAGTCTTT TGTACAGTGG 
TTAGGGTGTC GTAGTCATTC TTCAGCACAA TTCTTAATTG GAGACCAAGA ACCCTGGGCC 
TTTAGAGGTG GTCTAGCAGG AGAGTTCCAG CGTTTGCTGC CAATTGATGG GGCAAATGAT 
CTCTTTTTTC AGCCACCTCC ACTGACTCCT ACCTCCAAAG TTTATACTAT CAGACCTTAT 
TTTCCTAAGG ATGAGGCATC CGTGTACAAG ATTTGCAGAG AAATGTATGA CGATGGAGTG 
GGTTTACCCT TTCAAAGTCA GCCTGATCTT ATTGGAGACA AGTTAGTAGG AGGGCTGCTT 
TCCCTCAGCC TGGATTACTG CTTTGTCCTA GAAGATGAAG ATGGCATATG TGGTTATGCC 
TTGGGCACTG TAGATGTGAC CCCCTTTATT AAAAAATGTA AAATTTCCTG GATCCCCTTC 
ATGCAGGAGA AGTATACCAA GCCAAATGGT GACAAGGAAC TCTCTGAGGC TGAGAAAATA 
ATGTTGAGTT TCCATGAAGA ACAGGAAGTA CTGCCAGAAA CTTTCCTTGC TAATTTCCCT 
TCTCTGATAA AGATGGACAT TCACAAAAAA GTAACTGACC CAAGTGTGGC CAAAAGCATG 
ATGGCTTGCC TCCTGTCTTC ACTGAAGGCT AATGGCTCCC GGGGAGCTTT CTGTGAAGTG 
AGACCAGATG ATAAAAGAAT TCTGGAATTT TACAGCAAGT TAGGATGTTT TGAAATTGCA 
AAAATGGAAG GATTTCCAAA GGATGTGGTT ATACTTGGTC GGAGCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OGA ( NM_012215.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036347.1 Homo sapiens O-GlcNAcase (OGA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06111
Clone ID Related Accession (Same CDS sequence)	NM_012215.3 , NM_012215.4 , NM_012215.5
Accession Version	NM_012215.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	protein O-GlcNAcase isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC353958.1, BC047877.1, AB014579.2 and BQ016159.1. On Nov 22, 2018 this sequence version replaced NM_012215.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF036144.2, SRR1803616.4396.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGCAGA AGGAGAGTCA AGCGACGTTG GAGGAGCGGG AGAGCGAGCT CAGCTCCAAC 
CCTGCCGCCT CTGCGGGGGC ATCGCTGGAG CCGCCGGCAG CTCCGGCACC CGGAGAAGAC 
AACCCCGCCG GGGCTGGGGG AGCGGCGGTG GCCGGGGCTG CAGGAGGGGC TCGGCGGTTC 
CTCTGCGGTG TGGTGGAAGG ATTTTATGGA AGACCTTGGG TTATGGAACA GAGAAAAGAA 
CTCTTTAGAA GGCTCCAGAA ATGGGAATTA AATACATACT TGTATGCCCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AGGAAGCTGA GCAACTTATG 
ACTCTCATCT CTGCTGCACG AGAATATGAG ATAGAGTTCA TCTATGCGAT CTCACCTGGA 
TTGGATATCA CTTTTTCTAA CCCCAAGGAA GTATCCACAT TGAAACGTAA ATTGGACCAG 
GTTTCTCAGT TTGGGTGCAG ATCATTTGCT TTGCTTTTTG ATGATATAGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT ATTCAGTTCT TTTGCTCATG CCCAAGTCTC CATCACAAAT 
GAAATCTATC AGTACCTAGG AGAGCCAGAA ACTTTCCTCT TCTGTCCCAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTGTCTCAG TCTCCATATT TAAGGACTGT GGGTGAAAAG 
CTTCTACCTG GAATTGAAGT GCTTTGGACA GGTCCCAAAG TTGTTTCTAA AGAAATTCCA 
GTAGAGTCCA TCGAAGAGGT TTCTAAGATT ATTAAGAGAG CTCCAGTAAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGA CTGTTTCTGG GCCCGTACAA AGGAAGATCC 
ACAGAACTCA TCCCACGGTT AAAAGGAGTC CTCACTAATC CAAATTGTGA ATTTGAAGCC 
AACTACGTTG CTATCCACAC CCTTGCCACC TGGTACAAAT CAAACATGAA TGGAGTGAGA 
AAAGATGTAG TGATGACTGA CAGTGAAGAT AGTACTGTGT CCATCCAGAT AAAATTAGAA 
AATGAAGGCA GTGATGAAGA TATTGAAACT GATGTACTCT ATAGTCCACA GATGGCTCTA 
AAGCTAGCAT TAACAGAATG GTTGCAAGAG TTTGGTGTGC CTCATCAATA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAAGCAAGT GTAGTTGATG GGACTCCTTT AGTTGCAGCA 
CCCTCTTTAA ATGCCACAAC CGTAGTAACA ACAGTTTATC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCCT TGAGTGGTGA GCCTACTACT CTGACCAAGG AAGAAGAAAA GAAACAGCCT 
GATGAAGAAC CCATGGACAT GGTGGTGGAA AAACAAGAAG AAACGGACCA CAAGAATGAC 
AATCAAATAC TGAGTGAAAT TGTTGAAGCG AAAATGGCAG AGGAATTGAA ACCAATGGAC 
ACTGATAAAG AGAGCATAGC TGAATCAAAA TCCCCAGAGA TGTCCATGCA AGAAGATTGT 
ATTAGTGACA TTGCCCCCAT GCAAACTGAT GAACAGACAA ACAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTTT GTACACTGCG GAACCAGTGA CCCTGGAGGA TTTGCAGTTA 
CTTGCTGATC TATTCTACCT TCCTTACGAG CATGGACCCA AAGGAGCACA GATGTTACGG 
GAATTTCAAT GGCTTCGAGC AAATAGTAGT GTTGTCAGTG TCAATTGCAA AGGAAAAGAC 
TCTGAAAAAA TTGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGGACTA 
GTGATGGGAA TGTTCACTCG GCTCTCCAAT TGTGCCAACA GGACAATTCT TTATGACATG 
TACTCCTATG TTTGGGATAT CAAGAGTATA ATGTCTATGG TGAAGTCTTT TGTACAGTGG 
TTAGGGTGTC GTAGTCATTC TTCAGCACAA TTCTTAATTG GAGACCAAGA ACCCTGGGCC 
TTTAGAGGTG GTCTAGCAGG AGAGTTCCAG CGTTTGCTGC CAATTGATGG GGCAAATGAT 
CTCTTTTTTC AGCCACCTCC ACTGACTCCT ACCTCCAAAG TTTATACTAT CAGACCTTAT 
TTTCCTAAGG ATGAGGCATC CGTGTACAAG ATTTGCAGAG AAATGTATGA CGATGGAGTG 
GGTTTACCCT TTCAAAGTCA GCCTGATCTT ATTGGAGACA AGTTAGTAGG AGGGCTGCTT 
TCCCTCAGCC TGGATTACTG CTTTGTCCTA GAAGATGAAG ATGGCATATG TGGTTATGCC 
TTGGGCACTG TAGATGTGAC CCCCTTTATT AAAAAATGTA AAATTTCCTG GATCCCCTTC 
ATGCAGGAGA AGTATACCAA GCCAAATGGT GACAAGGAAC TCTCTGAGGC TGAGAAAATA 
ATGTTGAGTT TCCATGAAGA ACAGGAAGTA CTGCCAGAAA CTTTCCTTGC TAATTTCCCT 
TCTCTGATAA AGATGGACAT TCACAAAAAA GTAACTGACC CAAGTGTGGC CAAAAGCATG 
ATGGCTTGCC TCCTGTCTTC ACTGAAGGCT AATGGCTCCC GGGGAGCTTT CTGTGAAGTG 
AGACCAGATG ATAAAAGAAT TCTGGAATTT TACAGCAAGT TAGGATGTTT TGAAATTGCA 
AAAATGGAAG GATTTCCAAA GGATGTGGTT ATACTTGGTC GGAGCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036347.1
CDS	423..3173
Translation	MVQKESQATLEERESELSSNPAASAGASLEPPAAPAPGEDNPAGAGGAAVAGAAGGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKASVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPTTLTKEEEKKQPDEEPMDMVVEKQEETDHKNDNQILSEIVEAKMAEELKPMDTDKESIAESKSPEMSMQEDCISDIAPMQTDEQTNKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCGLVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGANDLFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGLPFQSQPDLIGDKLVGGLLSLSLDYCFVLEDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGRSL

Target ORF information:

RefSeq Version	NM_012215.4
Organism	Homo sapiens(human)
Definition	Homo sapiens O-GlcNAcase (OGA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012215.4

ORF Insert Sequence:

ATGGTGCAGA AGGAGAGTCA AGCGACGTTG GAGGAGCGGG AGAGCGAGCT CAGCTCCAAC 
CCTGCCGCCT CTGCGGGGGC ATCGCTGGAG CCGCCGGCAG CTCCGGCACC CGGAGAAGAC 
AACCCCGCCG GGGCTGGGGG AGCGGCGGTG GCCGGGGCTG CAGGAGGGGC TCGGCGGTTC 
CTCTGCGGTG TGGTGGAAGG ATTTTATGGA AGACCTTGGG TTATGGAACA GAGAAAAGAA 
CTCTTTAGAA GGCTCCAGAA ATGGGAATTA AATACATACT TGTATGCCCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AGGAAGCTGA GCAACTTATG 
ACTCTCATCT CTGCTGCACG AGAATATGAG ATAGAGTTCA TCTATGCGAT CTCACCTGGA 
TTGGATATCA CTTTTTCTAA CCCCAAGGAA GTATCCACAT TGAAACGTAA ATTGGACCAG 
GTTTCTCAGT TTGGGTGCAG ATCATTTGCT TTGCTTTTTG ATGATATAGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT ATTCAGTTCT TTTGCTCATG CCCAAGTCTC CATCACAAAT 
GAAATCTATC AGTACCTAGG AGAGCCAGAA ACTTTCCTCT TCTGTCCCAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTGTCTCAG TCTCCATATT TAAGGACTGT GGGTGAAAAG 
CTTCTACCTG GAATTGAAGT GCTTTGGACA GGTCCCAAAG TTGTTTCTAA AGAAATTCCA 
GTAGAGTCCA TCGAAGAGGT TTCTAAGATT ATTAAGAGAG CTCCAGTAAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGA CTGTTTCTGG GCCCGTACAA AGGAAGATCC 
ACAGAACTCA TCCCACGGTT AAAAGGAGTC CTCACTAATC CAAATTGTGA ATTTGAAGCC 
AACTACGTTG CTATCCACAC CCTTGCCACC TGGTACAAAT CAAACATGAA TGGAGTGAGA 
AAAGATGTAG TGATGACTGA CAGTGAAGAT AGTACTGTGT CCATCCAGAT AAAATTAGAA 
AATGAAGGCA GTGATGAAGA TATTGAAACT GATGTACTCT ATAGTCCACA GATGGCTCTA 
AAGCTAGCAT TAACAGAATG GTTGCAAGAG TTTGGTGTGC CTCATCAATA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAAGCAAGT GTAGTTGATG GGACTCCTTT AGTTGCAGCA 
CCCTCTTTAA ATGCCACAAC CGTAGTAACA ACAGTTTATC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCCT TGAGTGGTGA GCCTACTACT CTGACCAAGG AAGAAGAAAA GAAACAGCCT 
GATGAAGAAC CCATGGACAT GGTGGTGGAA AAACAAGAAG AAACGGACCA CAAGAATGAC 
AATCAAATAC TGAGTGAAAT TGTTGAAGCG AAAATGGCAG AGGAATTGAA ACCAATGGAC 
ACTGATAAAG AGAGCATAGC TGAATCAAAA TCCCCAGAGA TGTCCATGCA AGAAGATTGT 
ATTAGTGACA TTGCCCCCAT GCAAACTGAT GAACAGACAA ACAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTTT GTACACTGCG GAACCAGTGA CCCTGGAGGA TTTGCAGTTA 
CTTGCTGATC TATTCTACCT TCCTTACGAG CATGGACCCA AAGGAGCACA GATGTTACGG 
GAATTTCAAT GGCTTCGAGC AAATAGTAGT GTTGTCAGTG TCAATTGCAA AGGAAAAGAC 
TCTGAAAAAA TTGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGGACTA 
GTGATGGGAA TGTTCACTCG GCTCTCCAAT TGTGCCAACA GGACAATTCT TTATGACATG 
TACTCCTATG TTTGGGATAT CAAGAGTATA ATGTCTATGG TGAAGTCTTT TGTACAGTGG 
TTAGGGTGTC GTAGTCATTC TTCAGCACAA TTCTTAATTG GAGACCAAGA ACCCTGGGCC 
TTTAGAGGTG GTCTAGCAGG AGAGTTCCAG CGTTTGCTGC CAATTGATGG GGCAAATGAT 
CTCTTTTTTC AGCCACCTCC ACTGACTCCT ACCTCCAAAG TTTATACTAT CAGACCTTAT 
TTTCCTAAGG ATGAGGCATC CGTGTACAAG ATTTGCAGAG AAATGTATGA CGATGGAGTG 
GGTTTACCCT TTCAAAGTCA GCCTGATCTT ATTGGAGACA AGTTAGTAGG AGGGCTGCTT 
TCCCTCAGCC TGGATTACTG CTTTGTCCTA GAAGATGAAG ATGGCATATG TGGTTATGCC 
TTGGGCACTG TAGATGTGAC CCCCTTTATT AAAAAATGTA AAATTTCCTG GATCCCCTTC 
ATGCAGGAGA AGTATACCAA GCCAAATGGT GACAAGGAAC TCTCTGAGGC TGAGAAAATA 
ATGTTGAGTT TCCATGAAGA ACAGGAAGTA CTGCCAGAAA CTTTCCTTGC TAATTTCCCT 
TCTCTGATAA AGATGGACAT TCACAAAAAA GTAACTGACC CAAGTGTGGC CAAAAGCATG 
ATGGCTTGCC TCCTGTCTTC ACTGAAGGCT AATGGCTCCC GGGGAGCTTT CTGTGAAGTG 
AGACCAGATG ATAAAAGAAT TCTGGAATTT TACAGCAAGT TAGGATGTTT TGAAATTGCA 
AAAATGGAAG GATTTCCAAA GGATGTGGTT ATACTTGGTC GGAGCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OGA ( NM_012215.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_036347.1 Homo sapiens O-GlcNAcase (OGA), transcript variant 1, mRNA.

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CloneID	OHu06111
Clone ID Related Accession (Same CDS sequence)	NM_012215.3 , NM_012215.4 , NM_012215.5
Accession Version	NM_012215.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	protein O-GlcNAcase isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC010789.9. On Aug 31, 2019 this sequence version replaced NM_012215.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB014579.2, AF036144.2 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361464.8/ ENSP00000354850.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGCAGA AGGAGAGTCA AGCGACGTTG GAGGAGCGGG AGAGCGAGCT CAGCTCCAAC 
CCTGCCGCCT CTGCGGGGGC ATCGCTGGAG CCGCCGGCAG CTCCGGCACC CGGAGAAGAC 
AACCCCGCCG GGGCTGGGGG AGCGGCGGTG GCCGGGGCTG CAGGAGGGGC TCGGCGGTTC 
CTCTGCGGTG TGGTGGAAGG ATTTTATGGA AGACCTTGGG TTATGGAACA GAGAAAAGAA 
CTCTTTAGAA GGCTCCAGAA ATGGGAATTA AATACATACT TGTATGCCCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AGGAAGCTGA GCAACTTATG 
ACTCTCATCT CTGCTGCACG AGAATATGAG ATAGAGTTCA TCTATGCGAT CTCACCTGGA 
TTGGATATCA CTTTTTCTAA CCCCAAGGAA GTATCCACAT TGAAACGTAA ATTGGACCAG 
GTTTCTCAGT TTGGGTGCAG ATCATTTGCT TTGCTTTTTG ATGATATAGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT ATTCAGTTCT TTTGCTCATG CCCAAGTCTC CATCACAAAT 
GAAATCTATC AGTACCTAGG AGAGCCAGAA ACTTTCCTCT TCTGTCCCAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTGTCTCAG TCTCCATATT TAAGGACTGT GGGTGAAAAG 
CTTCTACCTG GAATTGAAGT GCTTTGGACA GGTCCCAAAG TTGTTTCTAA AGAAATTCCA 
GTAGAGTCCA TCGAAGAGGT TTCTAAGATT ATTAAGAGAG CTCCAGTAAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGA CTGTTTCTGG GCCCGTACAA AGGAAGATCC 
ACAGAACTCA TCCCACGGTT AAAAGGAGTC CTCACTAATC CAAATTGTGA ATTTGAAGCC 
AACTACGTTG CTATCCACAC CCTTGCCACC TGGTACAAAT CAAACATGAA TGGAGTGAGA 
AAAGATGTAG TGATGACTGA CAGTGAAGAT AGTACTGTGT CCATCCAGAT AAAATTAGAA 
AATGAAGGCA GTGATGAAGA TATTGAAACT GATGTACTCT ATAGTCCACA GATGGCTCTA 
AAGCTAGCAT TAACAGAATG GTTGCAAGAG TTTGGTGTGC CTCATCAATA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAAGCAAGT GTAGTTGATG GGACTCCTTT AGTTGCAGCA 
CCCTCTTTAA ATGCCACAAC CGTAGTAACA ACAGTTTATC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCCT TGAGTGGTGA GCCTACTACT CTGACCAAGG AAGAAGAAAA GAAACAGCCT 
GATGAAGAAC CCATGGACAT GGTGGTGGAA AAACAAGAAG AAACGGACCA CAAGAATGAC 
AATCAAATAC TGAGTGAAAT TGTTGAAGCG AAAATGGCAG AGGAATTGAA ACCAATGGAC 
ACTGATAAAG AGAGCATAGC TGAATCAAAA TCCCCAGAGA TGTCCATGCA AGAAGATTGT 
ATTAGTGACA TTGCCCCCAT GCAAACTGAT GAACAGACAA ACAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTTT GTACACTGCG GAACCAGTGA CCCTGGAGGA TTTGCAGTTA 
CTTGCTGATC TATTCTACCT TCCTTACGAG CATGGACCCA AAGGAGCACA GATGTTACGG 
GAATTTCAAT GGCTTCGAGC AAATAGTAGT GTTGTCAGTG TCAATTGCAA AGGAAAAGAC 
TCTGAAAAAA TTGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGGACTA 
GTGATGGGAA TGTTCACTCG GCTCTCCAAT TGTGCCAACA GGACAATTCT TTATGACATG 
TACTCCTATG TTTGGGATAT CAAGAGTATA ATGTCTATGG TGAAGTCTTT TGTACAGTGG 
TTAGGGTGTC GTAGTCATTC TTCAGCACAA TTCTTAATTG GAGACCAAGA ACCCTGGGCC 
TTTAGAGGTG GTCTAGCAGG AGAGTTCCAG CGTTTGCTGC CAATTGATGG GGCAAATGAT 
CTCTTTTTTC AGCCACCTCC ACTGACTCCT ACCTCCAAAG TTTATACTAT CAGACCTTAT 
TTTCCTAAGG ATGAGGCATC CGTGTACAAG ATTTGCAGAG AAATGTATGA CGATGGAGTG 
GGTTTACCCT TTCAAAGTCA GCCTGATCTT ATTGGAGACA AGTTAGTAGG AGGGCTGCTT 
TCCCTCAGCC TGGATTACTG CTTTGTCCTA GAAGATGAAG ATGGCATATG TGGTTATGCC 
TTGGGCACTG TAGATGTGAC CCCCTTTATT AAAAAATGTA AAATTTCCTG GATCCCCTTC 
ATGCAGGAGA AGTATACCAA GCCAAATGGT GACAAGGAAC TCTCTGAGGC TGAGAAAATA 
ATGTTGAGTT TCCATGAAGA ACAGGAAGTA CTGCCAGAAA CTTTCCTTGC TAATTTCCCT 
TCTCTGATAA AGATGGACAT TCACAAAAAA GTAACTGACC CAAGTGTGGC CAAAAGCATG 
ATGGCTTGCC TCCTGTCTTC ACTGAAGGCT AATGGCTCCC GGGGAGCTTT CTGTGAAGTG 
AGACCAGATG ATAAAAGAAT TCTGGAATTT TACAGCAAGT TAGGATGTTT TGAAATTGCA 
AAAATGGAAG GATTTCCAAA GGATGTGGTT ATACTTGGTC GGAGCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036347.1
CDS	423..3173
Translation	MVQKESQATLEERESELSSNPAASAGASLEPPAAPAPGEDNPAGAGGAAVAGAAGGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKASVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPTTLTKEEEKKQPDEEPMDMVVEKQEETDHKNDNQILSEIVEAKMAEELKPMDTDKESIAESKSPEMSMQEDCISDIAPMQTDEQTNKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCGLVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGANDLFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGLPFQSQPDLIGDKLVGGLLSLSLDYCFVLEDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGRSL

Target ORF information:

RefSeq Version	NM_012215.5
Organism	Homo sapiens(human)
Definition	Homo sapiens O-GlcNAcase (OGA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012215.5

ORF Insert Sequence:

ATGGTGCAGA AGGAGAGTCA AGCGACGTTG GAGGAGCGGG AGAGCGAGCT CAGCTCCAAC 
CCTGCCGCCT CTGCGGGGGC ATCGCTGGAG CCGCCGGCAG CTCCGGCACC CGGAGAAGAC 
AACCCCGCCG GGGCTGGGGG AGCGGCGGTG GCCGGGGCTG CAGGAGGGGC TCGGCGGTTC 
CTCTGCGGTG TGGTGGAAGG ATTTTATGGA AGACCTTGGG TTATGGAACA GAGAAAAGAA 
CTCTTTAGAA GGCTCCAGAA ATGGGAATTA AATACATACT TGTATGCCCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AGGAAGCTGA GCAACTTATG 
ACTCTCATCT CTGCTGCACG AGAATATGAG ATAGAGTTCA TCTATGCGAT CTCACCTGGA 
TTGGATATCA CTTTTTCTAA CCCCAAGGAA GTATCCACAT TGAAACGTAA ATTGGACCAG 
GTTTCTCAGT TTGGGTGCAG ATCATTTGCT TTGCTTTTTG ATGATATAGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT ATTCAGTTCT TTTGCTCATG CCCAAGTCTC CATCACAAAT 
GAAATCTATC AGTACCTAGG AGAGCCAGAA ACTTTCCTCT TCTGTCCCAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTGTCTCAG TCTCCATATT TAAGGACTGT GGGTGAAAAG 
CTTCTACCTG GAATTGAAGT GCTTTGGACA GGTCCCAAAG TTGTTTCTAA AGAAATTCCA 
GTAGAGTCCA TCGAAGAGGT TTCTAAGATT ATTAAGAGAG CTCCAGTAAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGA CTGTTTCTGG GCCCGTACAA AGGAAGATCC 
ACAGAACTCA TCCCACGGTT AAAAGGAGTC CTCACTAATC CAAATTGTGA ATTTGAAGCC 
AACTACGTTG CTATCCACAC CCTTGCCACC TGGTACAAAT CAAACATGAA TGGAGTGAGA 
AAAGATGTAG TGATGACTGA CAGTGAAGAT AGTACTGTGT CCATCCAGAT AAAATTAGAA 
AATGAAGGCA GTGATGAAGA TATTGAAACT GATGTACTCT ATAGTCCACA GATGGCTCTA 
AAGCTAGCAT TAACAGAATG GTTGCAAGAG TTTGGTGTGC CTCATCAATA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAAGCAAGT GTAGTTGATG GGACTCCTTT AGTTGCAGCA 
CCCTCTTTAA ATGCCACAAC CGTAGTAACA ACAGTTTATC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCCT TGAGTGGTGA GCCTACTACT CTGACCAAGG AAGAAGAAAA GAAACAGCCT 
GATGAAGAAC CCATGGACAT GGTGGTGGAA AAACAAGAAG AAACGGACCA CAAGAATGAC 
AATCAAATAC TGAGTGAAAT TGTTGAAGCG AAAATGGCAG AGGAATTGAA ACCAATGGAC 
ACTGATAAAG AGAGCATAGC TGAATCAAAA TCCCCAGAGA TGTCCATGCA AGAAGATTGT 
ATTAGTGACA TTGCCCCCAT GCAAACTGAT GAACAGACAA ACAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTTT GTACACTGCG GAACCAGTGA CCCTGGAGGA TTTGCAGTTA 
CTTGCTGATC TATTCTACCT TCCTTACGAG CATGGACCCA AAGGAGCACA GATGTTACGG 
GAATTTCAAT GGCTTCGAGC AAATAGTAGT GTTGTCAGTG TCAATTGCAA AGGAAAAGAC 
TCTGAAAAAA TTGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGGACTA 
GTGATGGGAA TGTTCACTCG GCTCTCCAAT TGTGCCAACA GGACAATTCT TTATGACATG 
TACTCCTATG TTTGGGATAT CAAGAGTATA ATGTCTATGG TGAAGTCTTT TGTACAGTGG 
TTAGGGTGTC GTAGTCATTC TTCAGCACAA TTCTTAATTG GAGACCAAGA ACCCTGGGCC 
TTTAGAGGTG GTCTAGCAGG AGAGTTCCAG CGTTTGCTGC CAATTGATGG GGCAAATGAT 
CTCTTTTTTC AGCCACCTCC ACTGACTCCT ACCTCCAAAG TTTATACTAT CAGACCTTAT 
TTTCCTAAGG ATGAGGCATC CGTGTACAAG ATTTGCAGAG AAATGTATGA CGATGGAGTG 
GGTTTACCCT TTCAAAGTCA GCCTGATCTT ATTGGAGACA AGTTAGTAGG AGGGCTGCTT 
TCCCTCAGCC TGGATTACTG CTTTGTCCTA GAAGATGAAG ATGGCATATG TGGTTATGCC 
TTGGGCACTG TAGATGTGAC CCCCTTTATT AAAAAATGTA AAATTTCCTG GATCCCCTTC 
ATGCAGGAGA AGTATACCAA GCCAAATGGT GACAAGGAAC TCTCTGAGGC TGAGAAAATA 
ATGTTGAGTT TCCATGAAGA ACAGGAAGTA CTGCCAGAAA CTTTCCTTGC TAATTTCCCT 
TCTCTGATAA AGATGGACAT TCACAAAAAA GTAACTGACC CAAGTGTGGC CAAAAGCATG 
ATGGCTTGCC TCCTGTCTTC ACTGAAGGCT AATGGCTCCC GGGGAGCTTT CTGTGAAGTG 
AGACCAGATG ATAAAAGAAT TCTGGAATTT TACAGCAAGT TAGGATGTTT TGAAATTGCA 
AAAATGGAAG GATTTCCAAA GGATGTGGTT ATACTTGGTC GGAGCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OGA ( NM_001142434.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135906.1 Homo sapiens O-GlcNAcase (OGA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07523
Clone ID Related Accession (Same CDS sequence)	NM_001142434.1
Accession Version	NM_001142434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	protein O-GlcNAcase isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC353958.1, BC039583.2, AB014579.2 and BQ016159.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region, compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGTGCAGA AGGAGAGTCA AGCGACGTTG GAGGAGCGGG AGAGCGAGCT CAGCTCCAAC 
CCTGCCGCCT CTGCGGGGGC ATCGCTGGAG CCGCCGGCAG CTCCGGCACC CGGAGAAGAC 
AACCCCGCCG GGGCTGGGGG AGCGGCGGTG GCCGGGGCTG CAGGAGGGGC TCGGCGGTTC 
CTCTGCGGTG TGGTGGAAGG ATTTTATGGA AGACCTTGGG TTATGGAACA GAGAAAAGAA 
CTCTTTAGAA GGCTCCAGAA ATGGGAATTA AATACATACT TGTATGCCCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AGGAAGCTGA GCAACTTATG 
ACTCTCATCT CTGCTGCACG AGAATATGAG ATAGAGTTCA TCTATGCGAT CTCACCTGGA 
TTGGATATCA CTTTTTCTAA CCCCAAGGAA GTATCCACAT TGAAACGTAA ATTGGACCAG 
GTTTCTCAGT TTGGGTGCAG ATCATTTGCT TTGCTTTTTG ATGATATAGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT ATTCAGTTCT TTTGCTCATG CCCAAGTCTC CATCACAAAT 
GAAATCTATC AGTACCTAGG AGAGCCAGAA ACTTTCCTCT TCTGTCCCAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTGTCTCAG TCTCCATATT TAAGGACTGT GGGTGAAAAG 
CTTCTACCTG GAATTGAAGT GCTTTGGACA GGTCCCAAAG TTGTTTCTAA AGAAATTCCA 
GTAGAGTCCA TCGAAGAGGT TTCTAAGATT ATTAAGAGAG CTCCAGTAAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGA CTGTTTCTGG GCCCGTACAA AGGAAGATCC 
ACAGAACTCA TCCCACGGTT AAAAGGAGTC CTCACTAATC CAAATTGTGA ATTTGAAGCC 
AACTACGTTG CTATCCACAC CCTTGCCACC TGGTACAAAT CAAACATGAA TGGAGTGAGA 
AAAGATGTAG TGATGAGTAG GCAAGTTGCA CACAGTGGAG CTAAAGCAAG TGTAGTTGAT 
GGGACTCCTT TAGTTGCAGC ACCCTCTTTA AATGCCACAA CCGTAGTAAC AACAGTTTAT 
CAGGAGCCCA TTATGAGCCA GGGAGCAGCC TTGAGTGGTG AGCCTACTAC TCTGACCAAG 
GAAGAAGAAA AGAAACAGCC TGATGAAGAA CCCATGGACA TGGTGGTGGA AAAACAAGAA 
GAAACGGACC ACAAGAATGA CAATCAAATA CTGAGTGAAA TTGTTGAAGC GAAAATGGCA 
GAGGAATTGA AACCAATGGA CACTGATAAA GAGAGCATAG CTGAATCAAA ATCCCCAGAG 
ATGTCCATGC AAGAAGATTG TATTAGTGAC ATTGCCCCCA TGCAAACTGA TGAACAGACA 
AACAAGGAGC AGTTTGTGCC AGGTCCAAAT GAAAAGCCTT TGTACACTGC GGAACCAGTG 
ACCCTGGAGG ATTTGCAGTT ACTTGCTGAT CTATTCTACC TTCCTTACGA GCATGGACCC 
AAAGGAGCAC AGATGTTACG GGAATTTCAA TGGCTTCGAG CAAATAGTAG TGTTGTCAGT 
GTCAATTGCA AAGGAAAAGA CTCTGAAAAA ATTGAAGAAT GGCGGTCACG AGCAGCCAAG 
TTTGAAGAGA TGTGTGGACT AGTGATGGGA ATGTTCACTC GGCTCTCCAA TTGTGCCAAC 
AGGACAATTC TTTATGACAT GTACTCCTAT GTTTGGGATA TCAAGAGTAT AATGTCTATG 
GTGAAGTCTT TTGTACAGTG GTTAGGGTGT CGTAGTCATT CTTCAGCACA ATTCTTAATT 
GGAGACCAAG AACCCTGGGC CTTTAGAGGT GGTCTAGCAG GAGAGTTCCA GCGTTTGCTG 
CCAATTGATG GGGCAAATGA TCTCTTTTTT CAGCCACCTC CACTGACTCC TACCTCCAAA 
GTTTATACTA TCAGACCTTA TTTTCCTAAG GATGAGGCAT CCGTGTACAA GATTTGCAGA 
GAAATGTATG ACGATGGAGT GGGTTTACCC TTTCAAAGTC AGCCTGATCT TATTGGAGAC 
AAGTTAGTAG GAGGGCTGCT TTCCCTCAGC CTGGATTACT GCTTTGTCCT AGAAGATGAA 
GATGGCATAT GTGGTTATGC CTTGGGCACT GTAGATGTGA CCCCCTTTAT TAAAAAATGT 
AAAATTTCCT GGATCCCCTT CATGCAGGAG AAGTATACCA AGCCAAATGG TGACAAGGAA 
CTCTCTGAGG CTGAGAAAAT AATGTTGAGT TTCCATGAAG AACAGGAAGT ACTGCCAGAA 
ACTTTCCTTG CTAATTTCCC TTCTCTGATA AAGATGGACA TTCACAAAAA AGTAACTGAC 
CCAAGTGTGG CCAAAAGCAT GATGGCTTGC CTCCTGTCTT CACTGAAGGC TAATGGCTCC 
CGGGGAGCTT TCTGTGAAGT GAGACCAGAT GATAAAAGAA TTCTGGAATT TTACAGCAAG 
TTAGGATGTT TTGAAATTGC AAAAATGGAA GGATTTCCAA AGGATGTGGT TATACTTGGT 
CGGAGCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135906.1
CDS	444..3035
Translation	MVQKESQATLEERESELSSNPAASAGASLEPPAAPAPGEDNPAGAGGAAVAGAAGGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMSRQVAHSGAKASVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPTTLTKEEEKKQPDEEPMDMVVEKQEETDHKNDNQILSEIVEAKMAEELKPMDTDKESIAESKSPEMSMQEDCISDIAPMQTDEQTNKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCGLVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGANDLFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGLPFQSQPDLIGDKLVGGLLSLSLDYCFVLEDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGRSL

Target ORF information:

RefSeq Version	NM_001142434.1
Organism	Homo sapiens(human)
Definition	Homo sapiens O-GlcNAcase (OGA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142434.1

ORF Insert Sequence:

ATGGTGCAGA AGGAGAGTCA AGCGACGTTG GAGGAGCGGG AGAGCGAGCT CAGCTCCAAC 
CCTGCCGCCT CTGCGGGGGC ATCGCTGGAG CCGCCGGCAG CTCCGGCACC CGGAGAAGAC 
AACCCCGCCG GGGCTGGGGG AGCGGCGGTG GCCGGGGCTG CAGGAGGGGC TCGGCGGTTC 
CTCTGCGGTG TGGTGGAAGG ATTTTATGGA AGACCTTGGG TTATGGAACA GAGAAAAGAA 
CTCTTTAGAA GGCTCCAGAA ATGGGAATTA AATACATACT TGTATGCCCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AGGAAGCTGA GCAACTTATG 
ACTCTCATCT CTGCTGCACG AGAATATGAG ATAGAGTTCA TCTATGCGAT CTCACCTGGA 
TTGGATATCA CTTTTTCTAA CCCCAAGGAA GTATCCACAT TGAAACGTAA ATTGGACCAG 
GTTTCTCAGT TTGGGTGCAG ATCATTTGCT TTGCTTTTTG ATGATATAGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT ATTCAGTTCT TTTGCTCATG CCCAAGTCTC CATCACAAAT 
GAAATCTATC AGTACCTAGG AGAGCCAGAA ACTTTCCTCT TCTGTCCCAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTGTCTCAG TCTCCATATT TAAGGACTGT GGGTGAAAAG 
CTTCTACCTG GAATTGAAGT GCTTTGGACA GGTCCCAAAG TTGTTTCTAA AGAAATTCCA 
GTAGAGTCCA TCGAAGAGGT TTCTAAGATT ATTAAGAGAG CTCCAGTAAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGA CTGTTTCTGG GCCCGTACAA AGGAAGATCC 
ACAGAACTCA TCCCACGGTT AAAAGGAGTC CTCACTAATC CAAATTGTGA ATTTGAAGCC 
AACTACGTTG CTATCCACAC CCTTGCCACC TGGTACAAAT CAAACATGAA TGGAGTGAGA 
AAAGATGTAG TGATGAGTAG GCAAGTTGCA CACAGTGGAG CTAAAGCAAG TGTAGTTGAT 
GGGACTCCTT TAGTTGCAGC ACCCTCTTTA AATGCCACAA CCGTAGTAAC AACAGTTTAT 
CAGGAGCCCA TTATGAGCCA GGGAGCAGCC TTGAGTGGTG AGCCTACTAC TCTGACCAAG 
GAAGAAGAAA AGAAACAGCC TGATGAAGAA CCCATGGACA TGGTGGTGGA AAAACAAGAA 
GAAACGGACC ACAAGAATGA CAATCAAATA CTGAGTGAAA TTGTTGAAGC GAAAATGGCA 
GAGGAATTGA AACCAATGGA CACTGATAAA GAGAGCATAG CTGAATCAAA ATCCCCAGAG 
ATGTCCATGC AAGAAGATTG TATTAGTGAC ATTGCCCCCA TGCAAACTGA TGAACAGACA 
AACAAGGAGC AGTTTGTGCC AGGTCCAAAT GAAAAGCCTT TGTACACTGC GGAACCAGTG 
ACCCTGGAGG ATTTGCAGTT ACTTGCTGAT CTATTCTACC TTCCTTACGA GCATGGACCC 
AAAGGAGCAC AGATGTTACG GGAATTTCAA TGGCTTCGAG CAAATAGTAG TGTTGTCAGT 
GTCAATTGCA AAGGAAAAGA CTCTGAAAAA ATTGAAGAAT GGCGGTCACG AGCAGCCAAG 
TTTGAAGAGA TGTGTGGACT AGTGATGGGA ATGTTCACTC GGCTCTCCAA TTGTGCCAAC 
AGGACAATTC TTTATGACAT GTACTCCTAT GTTTGGGATA TCAAGAGTAT AATGTCTATG 
GTGAAGTCTT TTGTACAGTG GTTAGGGTGT CGTAGTCATT CTTCAGCACA ATTCTTAATT 
GGAGACCAAG AACCCTGGGC CTTTAGAGGT GGTCTAGCAG GAGAGTTCCA GCGTTTGCTG 
CCAATTGATG GGGCAAATGA TCTCTTTTTT CAGCCACCTC CACTGACTCC TACCTCCAAA 
GTTTATACTA TCAGACCTTA TTTTCCTAAG GATGAGGCAT CCGTGTACAA GATTTGCAGA 
GAAATGTATG ACGATGGAGT GGGTTTACCC TTTCAAAGTC AGCCTGATCT TATTGGAGAC 
AAGTTAGTAG GAGGGCTGCT TTCCCTCAGC CTGGATTACT GCTTTGTCCT AGAAGATGAA 
GATGGCATAT GTGGTTATGC CTTGGGCACT GTAGATGTGA CCCCCTTTAT TAAAAAATGT 
AAAATTTCCT GGATCCCCTT CATGCAGGAG AAGTATACCA AGCCAAATGG TGACAAGGAA 
CTCTCTGAGG CTGAGAAAAT AATGTTGAGT TTCCATGAAG AACAGGAAGT ACTGCCAGAA 
ACTTTCCTTG CTAATTTCCC TTCTCTGATA AAGATGGACA TTCACAAAAA AGTAACTGAC 
CCAAGTGTGG CCAAAAGCAT GATGGCTTGC CTCCTGTCTT CACTGAAGGC TAATGGCTCC 
CGGGGAGCTT TCTGTGAAGT GAGACCAGAT GATAAAAGAA TTCTGGAATT TTACAGCAAG 
TTAGGATGTT TTGAAATTGC AAAAATGGAA GGATTTCCAA AGGATGTGGT TATACTTGGT 
CGGAGCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OGA ( XM_017015585.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871074.1 Homo sapiens O-GlcNAcase (OGA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu64331
Clone ID Related Accession (Same CDS sequence)	XM_017015585.1
Accession Version	XM_017015585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein O-GlcNAcase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAC TTATGACTCT CATCTCTGCT GCACGAGAAT ATGAGATAGA GTTCATCTAT 
GCGATCTCAC CTGGATTGGA TATCACTTTT TCTAACCCCA AGGAAGTATC CACATTGAAA 
CGTAAATTGG ACCAGGTTTC TCAGTTTGGG TGCAGATCAT TTGCTTTGCT TTTTGATGAT 
ATAGACCATA ATATGTGTGC AGCAGACAAA GAGGTATTCA GTTCTTTTGC TCATGCCCAA 
GTCTCCATCA CAAATGAAAT CTATCAGTAC CTAGGAGAGC CAGAAACTTT CCTCTTCTGT 
CCCACAGAAT ACTGTGGCAC TTTCTGTTAT CCAAATGTGT CTCAGTCTCC ATATTTAAGG 
ACTGTGGGTG AAAAGCTTCT ACCTGGAATT GAAGTGCTTT GGACAGGTCC CAAAGTTGTT 
TCTAAAGAAA TTCCAGTAGA GTCCATCGAA GAGGTTTCTA AGATTATTAA GAGAGCTCCA 
GTAATCTGGG ATAACATTCA TGCTAATGAT TATGATCAGA AGAGACTGTT TCTGGGCCCG 
TACAAAGGAA GATCCACAGA ACTCATCCCA CGGTTAAAAG GAGTCCTCAC TAATCCAAAT 
TGTGAATTTG AAGCCAACTA CGTTGCTATC CACACCCTTG CCACCTGGTA CAAATCAAAC 
ATGAATGGAG TGAGAAAAGA TGTAGTGATG ACTGACAGTG AAGATAGTAC TGTGTCCATC 
CAGATAAAAT TAGAAAATGA AGGCAGTGAT GAAGATATTG AAACTGATGT ACTCTATAGT 
CCACAGATGG CTCTAAAGCT AGCATTAACA GAATGGTTGC AAGAGTTTGG TGTGCCTCAT 
CAATACAGCA GTAGGCAAGT TGCACACAGT GGAGCTAAAG CAAGTGTAGT TGATGGGACT 
CCTTTAGTTG CAGCACCCTC TTTAAATGCC ACAACCGTAG TAACAACAGT TTATCAGGAG 
CCCATTATGA GCCAGGGAGC AGCCTTGAGT GGTGAGCCTA CTACTCTGAC CAAGGAAGAA 
GAAAAGAAAC AGCCTGATGA AGAACCCATG GACATGGTGG TGGAAAAACA AGAAGAAACG 
GACCACAAGA ATGACAATCA AATACTGAGT GAAATTGTTG AAGCGAAAAT GGCAGAGGAA 
TTGAAACCAA TGGACACTGA TAAAGAGAGC ATAGCTGAAT CAAAATCCCC AGAGATGTCC 
ATGCAAGAAG ATTGTATTAG TGACATTGCC CCCATGCAAA CTGATGAACA GACAAACAAG 
GAGCAGTTTG TGCCAGGTCC AAATGAAAAG CCTTTGTACA CTGCGGAACC AGTGACCCTG 
GAGGATTTGC AGTTACTTGC TGATCTATTC TACCTTCCTT ACGAGCATGG ACCCAAAGGA 
GCACAGATGT TACGGGAATT TCAATGGCTT CGAGCAAATA GTAGTGTTGT CAGTGTCAAT 
TGCAAAGGAA AAGACTCTGA AAAAATTGAA GAATGGCGGT CACGAGCAGC CAAGTTTGAA 
GAGATGTGTG GACTAGTGAT GGGAATGTTC ACTCGGCTCT CCAATTGTGC CAACAGGACA 
ATTCTTTATG ACATGTACTC CTATGTTTGG GATATCAAGA GTATAATGTC TATGGTGAAG 
TCTTTTGTAC AGTGGTTAGG GTGTCGTAGT CATTCTTCAG CACAATTCTT AATTGGAGAC 
CAAGAACCCT GGGCCTTTAG AGGTGGTCTA GCAGGAGAGT TCCAGCGTTT GCTGCCAATT 
GATGGGGCAA ATGATCTCTT TTTTCAGCCA CCTCCACTGA CTCCTACCTC CAAAGTTTAT 
ACTATCAGAC CTTATTTTCC TAAGGATGAG GCATCCGTGT ACAAGATTTG CAGAGAAATG 
TATGACGATG GAGTGGGTTT ACCCTTTCAA AGTCAGCCTG ATCTTATTGG AGACAAGTTA 
GTAGGAGGGC TGCTTTCCCT CAGCCTGGAT TACTGCTTTG TCCTAGAAGA TGAAGATGGC 
ATATGTGGTT ATGCCTTGGG CACTGTAGAT GTGACCCCCT TTATTAAAAA ATGTAAAATT 
TCCTGGATCC CCTTCATGCA GGAGAAGTAT ACCAAGCCAA ATGGTGACAA GGAACTCTCT 
GAGGCTGAGA AAATAATGTT GAGTTTCCAT GAAGAACAGG AAGTACTGCC AGAAACTTTC 
CTTGCTAATT TCCCTTCTCT GATAAAGATG GACATTCACA AAAAAGTAAC TGACCCAAGT 
GTGGCCAAAA GCATGATGGC TTGCCTCCTG TCTTCACTGA AGGCTAATGG CTCCCGGGGA 
GCTTTCTGTG AAGTGAGACC AGATGATAAA AGAATTCTGG AATTTTACAG CAAGTTAGGA 
TGTTTTGAAA TTGCAAAAAT GGAAGGATTT CCAAAGGATG TGGTTATACT TGGTCGGAGC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871074.1
CDS	1166..3571
Translation	MEQLMTLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKASVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPTTLTKEEEKKQPDEEPMDMVVEKQEETDHKNDNQILSEIVEAKMAEELKPMDTDKESIAESKSPEMSMQEDCISDIAPMQTDEQTNKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCGLVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGANDLFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGLPFQSQPDLIGDKLVGGLLSLSLDYCFVLEDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGRSL

Target ORF information:

RefSeq Version	XM_017015585.1
Organism	Homo sapiens(human)
Definition	Homo sapiens O-GlcNAcase (OGA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015585.1

ORF Insert Sequence:

ATGGAGCAAC TTATGACTCT CATCTCTGCT GCACGAGAAT ATGAGATAGA GTTCATCTAT 
GCGATCTCAC CTGGATTGGA TATCACTTTT TCTAACCCCA AGGAAGTATC CACATTGAAA 
CGTAAATTGG ACCAGGTTTC TCAGTTTGGG TGCAGATCAT TTGCTTTGCT TTTTGATGAT 
ATAGACCATA ATATGTGTGC AGCAGACAAA GAGGTATTCA GTTCTTTTGC TCATGCCCAA 
GTCTCCATCA CAAATGAAAT CTATCAGTAC CTAGGAGAGC CAGAAACTTT CCTCTTCTGT 
CCCACAGAAT ACTGTGGCAC TTTCTGTTAT CCAAATGTGT CTCAGTCTCC ATATTTAAGG 
ACTGTGGGTG AAAAGCTTCT ACCTGGAATT GAAGTGCTTT GGACAGGTCC CAAAGTTGTT 
TCTAAAGAAA TTCCAGTAGA GTCCATCGAA GAGGTTTCTA AGATTATTAA GAGAGCTCCA 
GTAATCTGGG ATAACATTCA TGCTAATGAT TATGATCAGA AGAGACTGTT TCTGGGCCCG 
TACAAAGGAA GATCCACAGA ACTCATCCCA CGGTTAAAAG GAGTCCTCAC TAATCCAAAT 
TGTGAATTTG AAGCCAACTA CGTTGCTATC CACACCCTTG CCACCTGGTA CAAATCAAAC 
ATGAATGGAG TGAGAAAAGA TGTAGTGATG ACTGACAGTG AAGATAGTAC TGTGTCCATC 
CAGATAAAAT TAGAAAATGA AGGCAGTGAT GAAGATATTG AAACTGATGT ACTCTATAGT 
CCACAGATGG CTCTAAAGCT AGCATTAACA GAATGGTTGC AAGAGTTTGG TGTGCCTCAT 
CAATACAGCA GTAGGCAAGT TGCACACAGT GGAGCTAAAG CAAGTGTAGT TGATGGGACT 
CCTTTAGTTG CAGCACCCTC TTTAAATGCC ACAACCGTAG TAACAACAGT TTATCAGGAG 
CCCATTATGA GCCAGGGAGC AGCCTTGAGT GGTGAGCCTA CTACTCTGAC CAAGGAAGAA 
GAAAAGAAAC AGCCTGATGA AGAACCCATG GACATGGTGG TGGAAAAACA AGAAGAAACG 
GACCACAAGA ATGACAATCA AATACTGAGT GAAATTGTTG AAGCGAAAAT GGCAGAGGAA 
TTGAAACCAA TGGACACTGA TAAAGAGAGC ATAGCTGAAT CAAAATCCCC AGAGATGTCC 
ATGCAAGAAG ATTGTATTAG TGACATTGCC CCCATGCAAA CTGATGAACA GACAAACAAG 
GAGCAGTTTG TGCCAGGTCC AAATGAAAAG CCTTTGTACA CTGCGGAACC AGTGACCCTG 
GAGGATTTGC AGTTACTTGC TGATCTATTC TACCTTCCTT ACGAGCATGG ACCCAAAGGA 
GCACAGATGT TACGGGAATT TCAATGGCTT CGAGCAAATA GTAGTGTTGT CAGTGTCAAT 
TGCAAAGGAA AAGACTCTGA AAAAATTGAA GAATGGCGGT CACGAGCAGC CAAGTTTGAA 
GAGATGTGTG GACTAGTGAT GGGAATGTTC ACTCGGCTCT CCAATTGTGC CAACAGGACA 
ATTCTTTATG ACATGTACTC CTATGTTTGG GATATCAAGA GTATAATGTC TATGGTGAAG 
TCTTTTGTAC AGTGGTTAGG GTGTCGTAGT CATTCTTCAG CACAATTCTT AATTGGAGAC 
CAAGAACCCT GGGCCTTTAG AGGTGGTCTA GCAGGAGAGT TCCAGCGTTT GCTGCCAATT 
GATGGGGCAA ATGATCTCTT TTTTCAGCCA CCTCCACTGA CTCCTACCTC CAAAGTTTAT 
ACTATCAGAC CTTATTTTCC TAAGGATGAG GCATCCGTGT ACAAGATTTG CAGAGAAATG 
TATGACGATG GAGTGGGTTT ACCCTTTCAA AGTCAGCCTG ATCTTATTGG AGACAAGTTA 
GTAGGAGGGC TGCTTTCCCT CAGCCTGGAT TACTGCTTTG TCCTAGAAGA TGAAGATGGC 
ATATGTGGTT ATGCCTTGGG CACTGTAGAT GTGACCCCCT TTATTAAAAA ATGTAAAATT 
TCCTGGATCC CCTTCATGCA GGAGAAGTAT ACCAAGCCAA ATGGTGACAA GGAACTCTCT 
GAGGCTGAGA AAATAATGTT GAGTTTCCAT GAAGAACAGG AAGTACTGCC AGAAACTTTC 
CTTGCTAATT TCCCTTCTCT GATAAAGATG GACATTCACA AAAAAGTAAC TGACCCAAGT 
GTGGCCAAAA GCATGATGGC TTGCCTCCTG TCTTCACTGA AGGCTAATGG CTCCCGGGGA 
GCTTTCTGTG AAGTGAGACC AGATGATAAA AGAATTCTGG AATTTTACAG CAAGTTAGGA 
TGTTTTGAAA TTGCAAAAAT GGAAGGATTT CCAAAGGATG TGGTTATACT TGGTCGGAGC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OGA ( XM_024447782.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303550.1 Homo sapiens O-GlcNAcase (OGA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu106417
Clone ID Related Accession (Same CDS sequence)	XM_024447782.1
Accession Version	XM_024447782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein O-GlcNAcase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGGAGAGTCA AGCGACGTTG GAGGAGCGGG AGAGCGAGCT CAGCTCCAAC 
CCTGCCGCCT CTGCGGGGGC ATCGCTGGAG CCGCCGGCAG CTCCGGCACC CGGAGAAGAC 
AACCCCGCCG GGGCTGGGGG AGCGGCGGTG GCCGGGGCTG CAGGAGGGGC TCGGCGGTTC 
CTCTGCGGTG TGGTGGAAGG ATTTTATGGA AGACCTTGGG TTATGGAACA GAGAAAAGAA 
CTCTTTAGAA GGCTCCAGAA ATGGGAATTA AATACATACT TGTATGCCCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AGGAAGCTGA GCAACTTATG 
ACTCTCATCT CTGCTGCACG AGAATATGAG ATAGAGTTCA TCTATGCGAT CTCACCTGGA 
TTGGATATCA CTTTTTCTAA CCCCAAGGAA GTATCCACAT TGAAACGTAA ATTGGACCAG 
GTTTCTCAGT TTGGGTGCAG ATCATTTGCT TTGCTTTTTG ATGATATAGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT ATTCAGTTCT TTTGCTCATG CCCAAGTCTC CATCACAAAT 
GAAATCTATC AGTACCTAGG AGAGCCAGAA ACTTTCCTCT TCTGTCCCAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTGTCTCAG TCTCCATATT TAAGGACTGT GGGTGAAAAG 
CTTCTACCTG GAATTGAAGT GCTTTGGACA GGTCCCAAAG TTGTTTCTAA AGAAATTCCA 
GTAGAGTCCA TCGAAGAGGT TTCTAAGATT ATTAAGAGAG CTCCAGTAAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGA CTGTTTCTGG GCCCGTACAA AGGAAGATCC 
ACAGAACTCA TCCCACGGTT AAAAGGAGTC CTCACTAATC CAAATTGTGA ATTTGAAGCC 
AACTACGTTG CTATCCACAC CCTTGCCACC TGGTACAAAT CAAACATGAA TGGAGTGAGA 
AAAGATGTAG TGATGACTGA CAGTGAAGAT AGTACTGTGT CCATCCAGAT AAAATTAGAA 
AATGAAGGCA GTGATGAAGA TATTGAAACT GATGTACTCT ATAGTCCACA GATGGCTCTA 
AAGCTAGCAT TAACAGAATG GTTGCAAGAG TTTGGTGTGC CTCATCAATA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAAGCAAGT GTAGTTGATG GGACTCCTTT AGTTGCAGCA 
CCCTCTTTAA ATGCCACAAC CGTAGTAACA ACAGTTTATC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCCT TGAGTGGTGA GCCTACTACT CTGACCAAGG AAGAAGAAAA GAAACAGCCT 
GATGAAGAAC CCATGGACAT GGTGGTGGAA AAACAAGAAG AAACGGACCA CAAGAATGAC 
AATCAAATAC TGAGTGAAAT TGTTGAAGCG AAAATGGCAG AGGAATTGAA ACCAATGGAC 
ACTGATAAAG AGAGCATAGC TGAATCAAAA TCCCCAGAGA TGTCCATGCA AGAAGATTGT 
ATTAGTGACA TTGCCCCCAT GCAAACTGAT GAACAGACAA ACAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTTT GTACACTGCG GAACCAGTGA CCCTGGAGGA TTTGCAGTTA 
CTTGCTGATC TATTCTACCT TCCTTACGAG CATGGACCCA AAGGAGCACA GATGTTACGG 
GAATTTCAAT GGCTTCGAGC AAATAGTAGT GTTGTCAGTG TCAATTGCAA AGGAAAAGAC 
TCTGAAAAAA TTGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGGACTA 
GTGATGGGAA TGTTCACTCG GCTCTCCAAT TGTGCCAACA GGACAATTCT TTATGACATG 
TACTCCTATG TTTGGGATAT CAAGAGTATA ATGTCTATGG TGAAGTCTTT TGTACAGTGG 
TTAGCGTTTG CTGCCAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303550.1
CDS	423..2423
Translation	MVQKESQATLEERESELSSNPAASAGASLEPPAAPAPGEDNPAGAGGAAVAGAAGGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKASVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPTTLTKEEEKKQPDEEPMDMVVEKQEETDHKNDNQILSEIVEAKMAEELKPMDTDKESIAESKSPEMSMQEDCISDIAPMQTDEQTNKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCGLVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLAFAAN

Target ORF information:

RefSeq Version	XM_024447782.1
Organism	Homo sapiens(human)
Definition	Homo sapiens O-GlcNAcase (OGA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447782.1

ORF Insert Sequence:

ATGGTGCAGA AGGAGAGTCA AGCGACGTTG GAGGAGCGGG AGAGCGAGCT CAGCTCCAAC 
CCTGCCGCCT CTGCGGGGGC ATCGCTGGAG CCGCCGGCAG CTCCGGCACC CGGAGAAGAC 
AACCCCGCCG GGGCTGGGGG AGCGGCGGTG GCCGGGGCTG CAGGAGGGGC TCGGCGGTTC 
CTCTGCGGTG TGGTGGAAGG ATTTTATGGA AGACCTTGGG TTATGGAACA GAGAAAAGAA 
CTCTTTAGAA GGCTCCAGAA ATGGGAATTA AATACATACT TGTATGCCCC AAAAGATGAC 
TACAAACATA GGATGTTTTG GCGAGAGATG TATTCAGTGG AGGAAGCTGA GCAACTTATG 
ACTCTCATCT CTGCTGCACG AGAATATGAG ATAGAGTTCA TCTATGCGAT CTCACCTGGA 
TTGGATATCA CTTTTTCTAA CCCCAAGGAA GTATCCACAT TGAAACGTAA ATTGGACCAG 
GTTTCTCAGT TTGGGTGCAG ATCATTTGCT TTGCTTTTTG ATGATATAGA CCATAATATG 
TGTGCAGCAG ACAAAGAGGT ATTCAGTTCT TTTGCTCATG CCCAAGTCTC CATCACAAAT 
GAAATCTATC AGTACCTAGG AGAGCCAGAA ACTTTCCTCT TCTGTCCCAC AGAATACTGT 
GGCACTTTCT GTTATCCAAA TGTGTCTCAG TCTCCATATT TAAGGACTGT GGGTGAAAAG 
CTTCTACCTG GAATTGAAGT GCTTTGGACA GGTCCCAAAG TTGTTTCTAA AGAAATTCCA 
GTAGAGTCCA TCGAAGAGGT TTCTAAGATT ATTAAGAGAG CTCCAGTAAT CTGGGATAAC 
ATTCATGCTA ATGATTATGA TCAGAAGAGA CTGTTTCTGG GCCCGTACAA AGGAAGATCC 
ACAGAACTCA TCCCACGGTT AAAAGGAGTC CTCACTAATC CAAATTGTGA ATTTGAAGCC 
AACTACGTTG CTATCCACAC CCTTGCCACC TGGTACAAAT CAAACATGAA TGGAGTGAGA 
AAAGATGTAG TGATGACTGA CAGTGAAGAT AGTACTGTGT CCATCCAGAT AAAATTAGAA 
AATGAAGGCA GTGATGAAGA TATTGAAACT GATGTACTCT ATAGTCCACA GATGGCTCTA 
AAGCTAGCAT TAACAGAATG GTTGCAAGAG TTTGGTGTGC CTCATCAATA CAGCAGTAGG 
CAAGTTGCAC ACAGTGGAGC TAAAGCAAGT GTAGTTGATG GGACTCCTTT AGTTGCAGCA 
CCCTCTTTAA ATGCCACAAC CGTAGTAACA ACAGTTTATC AGGAGCCCAT TATGAGCCAG 
GGAGCAGCCT TGAGTGGTGA GCCTACTACT CTGACCAAGG AAGAAGAAAA GAAACAGCCT 
GATGAAGAAC CCATGGACAT GGTGGTGGAA AAACAAGAAG AAACGGACCA CAAGAATGAC 
AATCAAATAC TGAGTGAAAT TGTTGAAGCG AAAATGGCAG AGGAATTGAA ACCAATGGAC 
ACTGATAAAG AGAGCATAGC TGAATCAAAA TCCCCAGAGA TGTCCATGCA AGAAGATTGT 
ATTAGTGACA TTGCCCCCAT GCAAACTGAT GAACAGACAA ACAAGGAGCA GTTTGTGCCA 
GGTCCAAATG AAAAGCCTTT GTACACTGCG GAACCAGTGA CCCTGGAGGA TTTGCAGTTA 
CTTGCTGATC TATTCTACCT TCCTTACGAG CATGGACCCA AAGGAGCACA GATGTTACGG 
GAATTTCAAT GGCTTCGAGC AAATAGTAGT GTTGTCAGTG TCAATTGCAA AGGAAAAGAC 
TCTGAAAAAA TTGAAGAATG GCGGTCACGA GCAGCCAAGT TTGAAGAGAT GTGTGGACTA 
GTGATGGGAA TGTTCACTCG GCTCTCCAAT TGTGCCAACA GGACAATTCT TTATGACATG 
TACTCCTATG TTTGGGATAT CAAGAGTATA ATGTCTATGG TGAAGTCTTT TGTACAGTGG 
TTAGCGTTTG CTGCCAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OGA ( XM_017015586.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871075.1 Homo sapiens O-GlcNAcase (OGA), transcript variant X2, mRNA.

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CloneID	OHu91033
Clone ID Related Accession (Same CDS sequence)	XM_017015586.1
Accession Version	XM_017015586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein O-GlcNAcase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAAA AGATGACTAC AAACATAGGA TGTTTTGGCG AGAGATGTAT TCAGTGGAGG 
AAGCTGGTTT CTCAGTTTGG GTGCAGATCA TTTGCTTTGC TTTTTGATGA TATAGACCAT 
AATATGTGTG CAGCAGACAA AGAGGTATTC AGTTCTTTTG CTCATGCCCA AGTCTCCATC 
ACAAATGAAA TCTATCAGTA CCTAGGAGAG CCAGAAACTT TCCTCTTCTG TCCCACAGAA 
TACTGTGGCA CTTTCTGTTA TCCAAATGTG TCTCAGTCTC CATATTTAAG GACTGTGGGT 
GAAAAGCTTC TACCTGGAAT TGAAGTGCTT TGGACAGGTC CCAAAGTTGT TTCTAAAGAA 
ATTCCAGTAG AGTCCATCGA AGAGGTTTCT AAGATTATTA AGAGAGCTCC AGTAATCTGG 
GATAACATTC ATGCTAATGA TTATGATCAG AAGAGACTGT TTCTGGGCCC GTACAAAGGA 
AGATCCACAG AACTCATCCC ACGGTTAAAA GGAGTCCTCA CTAATCCAAA TTGTGAATTT 
GAAGCCAACT ACGTTGCTAT CCACACCCTT GCCACCTGGT ACAAATCAAA CATGAATGGA 
GTGAGAAAAG ATGTAGTGAT GACTGACAGT GAAGATAGTA CTGTGTCCAT CCAGATAAAA 
TTAGAAAATG AAGGCAGTGA TGAAGATATT GAAACTGATG TACTCTATAG TCCACAGATG 
GCTCTAAAGC TAGCATTAAC AGAATGGTTG CAAGAGTTTG GTGTGCCTCA TCAATACAGC 
AGTAGGCAAG TTGCACACAG TGGAGCTAAA GCAAGTGTAG TTGATGGGAC TCCTTTAGTT 
GCAGCACCCT CTTTAAATGC CACAACCGTA GTAACAACAG TTTATCAGGA GCCCATTATG 
AGCCAGGGAG CAGCCTTGAG TGGTGAGCCT ACTACTCTGA CCAAGGAAGA AGAAAAGAAA 
CAGCCTGATG AAGAACCCAT GGACATGGTG GTGGAAAAAC AAGAAGAAAC GGACCACAAG 
AATGACAATC AAATACTGAG TGAAATTGTT GAAGCGAAAA TGGCAGAGGA ATTGAAACCA 
ATGGACACTG ATAAAGAGAG CATAGCTGAA TCAAAATCCC CAGAGATGTC CATGCAAGAA 
GATTGTATTA GTGACATTGC CCCCATGCAA ACTGATGAAC AGACAAACAA GGAGCAGTTT 
GTGCCAGGTC CAAATGAAAA GCCTTTGTAC ACTGCGGAAC CAGTGACCCT GGAGGATTTG 
CAGTTACTTG CTGATCTATT CTACCTTCCT TACGAGCATG GACCCAAAGG AGCACAGATG 
TTACGGGAAT TTCAATGGCT TCGAGCAAAT AGTAGTGTTG TCAGTGTCAA TTGCAAAGGA 
AAAGACTCTG AAAAAATTGA AGAATGGCGG TCACGAGCAG CCAAGTTTGA AGAGATGTGT 
GGACTAGTGA TGGGAATGTT CACTCGGCTC TCCAATTGTG CCAACAGGAC AATTCTTTAT 
GACATGTACT CCTATGTTTG GGATATCAAG AGTATAATGT CTATGGTGAA GTCTTTTGTA 
CAGTGGTTAG GGTGTCGTAG TCATTCTTCA GCACAATTCT TAATTGGAGA CCAAGAACCC 
TGGGCCTTTA GAGGTGGTCT AGCAGGAGAG TTCCAGCGTT TGCTGCCAAT TGATGGGGCA 
AATGATCTCT TTTTTCAGCC ACCTCCACTG ACTCCTACCT CCAAAGTTTA TACTATCAGA 
CCTTATTTTC CTAAGGATGA GGCATCCGTG TACAAGATTT GCAGAGAAAT GTATGACGAT 
GGAGTGGGTT TACCCTTTCA AAGTCAGCCT GATCTTATTG GAGACAAGTT AGTAGGAGGG 
CTGCTTTCCC TCAGCCTGGA TTACTGCTTT GTCCTAGAAG ATGAAGATGG CATATGTGGT 
TATGCCTTGG GCACTGTAGA TGTGACCCCC TTTATTAAAA AATGTAAAAT TTCCTGGATC 
CCCTTCATGC AGGAGAAGTA TACCAAGCCA AATGGTGACA AGGAACTCTC TGAGGCTGAG 
AAAATAATGT TGAGTTTCCA TGAAGAACAG GAAGTACTGC CAGAAACTTT CCTTGCTAAT 
TTCCCTTCTC TGATAAAGAT GGACATTCAC AAAAAAGTAA CTGACCCAAG TGTGGCCAAA 
AGCATGATGG CTTGCCTCCT GTCTTCACTG AAGGCTAATG GCTCCCGGGG AGCTTTCTGT 
GAAGTGAGAC CAGATGATAA AAGAATTCTG GAATTTTACA GCAAGTTAGG ATGTTTTGAA 
ATTGCAAAAA TGGAAGGATT TCCAAAGGAT GTGGTTATAC TTGGTCGGAG CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871075.1
CDS	22..2358
Translation	MPQKMTTNIGCFGERCIQWRKLVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKASVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPTTLTKEEEKKQPDEEPMDMVVEKQEETDHKNDNQILSEIVEAKMAEELKPMDTDKESIAESKSPEMSMQEDCISDIAPMQTDEQTNKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCGLVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGANDLFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGLPFQSQPDLIGDKLVGGLLSLSLDYCFVLEDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGRSL

Target ORF information:

RefSeq Version	XM_017015586.1
Organism	Homo sapiens(human)
Definition	Homo sapiens O-GlcNAcase (OGA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015586.1

ORF Insert Sequence:

ATGCCCCAAA AGATGACTAC AAACATAGGA TGTTTTGGCG AGAGATGTAT TCAGTGGAGG 
AAGCTGGTTT CTCAGTTTGG GTGCAGATCA TTTGCTTTGC TTTTTGATGA TATAGACCAT 
AATATGTGTG CAGCAGACAA AGAGGTATTC AGTTCTTTTG CTCATGCCCA AGTCTCCATC 
ACAAATGAAA TCTATCAGTA CCTAGGAGAG CCAGAAACTT TCCTCTTCTG TCCCACAGAA 
TACTGTGGCA CTTTCTGTTA TCCAAATGTG TCTCAGTCTC CATATTTAAG GACTGTGGGT 
GAAAAGCTTC TACCTGGAAT TGAAGTGCTT TGGACAGGTC CCAAAGTTGT TTCTAAAGAA 
ATTCCAGTAG AGTCCATCGA AGAGGTTTCT AAGATTATTA AGAGAGCTCC AGTAATCTGG 
GATAACATTC ATGCTAATGA TTATGATCAG AAGAGACTGT TTCTGGGCCC GTACAAAGGA 
AGATCCACAG AACTCATCCC ACGGTTAAAA GGAGTCCTCA CTAATCCAAA TTGTGAATTT 
GAAGCCAACT ACGTTGCTAT CCACACCCTT GCCACCTGGT ACAAATCAAA CATGAATGGA 
GTGAGAAAAG ATGTAGTGAT GACTGACAGT GAAGATAGTA CTGTGTCCAT CCAGATAAAA 
TTAGAAAATG AAGGCAGTGA TGAAGATATT GAAACTGATG TACTCTATAG TCCACAGATG 
GCTCTAAAGC TAGCATTAAC AGAATGGTTG CAAGAGTTTG GTGTGCCTCA TCAATACAGC 
AGTAGGCAAG TTGCACACAG TGGAGCTAAA GCAAGTGTAG TTGATGGGAC TCCTTTAGTT 
GCAGCACCCT CTTTAAATGC CACAACCGTA GTAACAACAG TTTATCAGGA GCCCATTATG 
AGCCAGGGAG CAGCCTTGAG TGGTGAGCCT ACTACTCTGA CCAAGGAAGA AGAAAAGAAA 
CAGCCTGATG AAGAACCCAT GGACATGGTG GTGGAAAAAC AAGAAGAAAC GGACCACAAG 
AATGACAATC AAATACTGAG TGAAATTGTT GAAGCGAAAA TGGCAGAGGA ATTGAAACCA 
ATGGACACTG ATAAAGAGAG CATAGCTGAA TCAAAATCCC CAGAGATGTC CATGCAAGAA 
GATTGTATTA GTGACATTGC CCCCATGCAA ACTGATGAAC AGACAAACAA GGAGCAGTTT 
GTGCCAGGTC CAAATGAAAA GCCTTTGTAC ACTGCGGAAC CAGTGACCCT GGAGGATTTG 
CAGTTACTTG CTGATCTATT CTACCTTCCT TACGAGCATG GACCCAAAGG AGCACAGATG 
TTACGGGAAT TTCAATGGCT TCGAGCAAAT AGTAGTGTTG TCAGTGTCAA TTGCAAAGGA 
AAAGACTCTG AAAAAATTGA AGAATGGCGG TCACGAGCAG CCAAGTTTGA AGAGATGTGT 
GGACTAGTGA TGGGAATGTT CACTCGGCTC TCCAATTGTG CCAACAGGAC AATTCTTTAT 
GACATGTACT CCTATGTTTG GGATATCAAG AGTATAATGT CTATGGTGAA GTCTTTTGTA 
CAGTGGTTAG GGTGTCGTAG TCATTCTTCA GCACAATTCT TAATTGGAGA CCAAGAACCC 
TGGGCCTTTA GAGGTGGTCT AGCAGGAGAG TTCCAGCGTT TGCTGCCAAT TGATGGGGCA 
AATGATCTCT TTTTTCAGCC ACCTCCACTG ACTCCTACCT CCAAAGTTTA TACTATCAGA 
CCTTATTTTC CTAAGGATGA GGCATCCGTG TACAAGATTT GCAGAGAAAT GTATGACGAT 
GGAGTGGGTT TACCCTTTCA AAGTCAGCCT GATCTTATTG GAGACAAGTT AGTAGGAGGG 
CTGCTTTCCC TCAGCCTGGA TTACTGCTTT GTCCTAGAAG ATGAAGATGG CATATGTGGT 
TATGCCTTGG GCACTGTAGA TGTGACCCCC TTTATTAAAA AATGTAAAAT TTCCTGGATC 
CCCTTCATGC AGGAGAAGTA TACCAAGCCA AATGGTGACA AGGAACTCTC TGAGGCTGAG 
AAAATAATGT TGAGTTTCCA TGAAGAACAG GAAGTACTGC CAGAAACTTT CCTTGCTAAT 
TTCCCTTCTC TGATAAAGAT GGACATTCAC AAAAAAGTAA CTGACCCAAG TGTGGCCAAA 
AGCATGATGG CTTGCCTCCT GTCTTCACTG AAGGCTAATG GCTCCCGGGG AGCTTTCTGT 
GAAGTGAGAC CAGATGATAA AAGAATTCTG GAATTTTACA GCAAGTTAGG ATGTTTTGAA 
ATTGCAAAAA TGGAAGGATT TCCAAAGGAT GTGGTTATAC TTGGTCGGAG CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural insights into the substrate binding adaptability and specificity of human O-GlcNAcase.
Nature communications8(1)666(2017 09)
Li B,Li H,Hu CW,Jiang J

Hijacking of the O-GlcNAcZYME complex by the HTLV-1 Tax oncoprotein facilitates viral transcription.
PLoS pathogens13(7)e1006518(2017 Jul)
Groussaud D,Khair M,Tollenaere AI,Waast L,Kuo MS,Mangeney M,Martella C,Fardini Y,Coste S,Souidi M,Benit L,Pique C,Issad T

The Sulfur-Linked Analogue of O-GlcNAc (S-GlcNAc) Is an Enzymatically Stable and Reasonable Structural Surrogate for O-GlcNAc at the Peptide and Protein Levels.
Biochemistry56(27)3507-3517(2017 07)
De Leon CA,Levine PM,Craven TW,Pratt MR

Structures of human O-GlcNAcase and its complexes reveal a new substrate recognition mode.
Nature structural & molecular biology24(4)362-369(2017 Apr)
Li B,Li H,Lu L,Jiang J

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OGA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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