Cxxc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cxxc1
Entrez Gene ID	74322
Full Name	CXXC finger 1 (PHD domain)
Synonyms	2410002I16Rik,5830420C16Rik,AI426635,Cfp1,Cgbp,PHF18
General protein information	Preferred Names CXXC finger 1 (PHD domain) Names CXXC-type zinc finger protein 1 CpG binding protein PHD finger and CXXC domain-containing protein 1 cpG-binding protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 E2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006526302.3	XP_006526365.1	CXXC-type zinc finger protein 1 isoform X1
NM_028868.3	NP_083144.1	CXXC-type zinc finger protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CXXC1	NP_001095124.1
Mus musculus (house mouse)	Cxxc1	NP_083144.1
Rattus norvegicus (Norway rat)	Cxxc1	NP_001073166.1
Xenopus tropicalis (tropical clawed frog)	cxxc1	XP_002943289.2
Danio rerio (zebrafish)	cxxc1	NP_956627.1
Drosophila melanogaster (fruit fly)	Cfp1	NP_001188570.1
Canis lupus familiaris (dog)	CXXC1	XP_005623161.1
Bos taurus (cattle)	CXXC1	NP_001019688.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004704	XP_318129.4
Pan troglodytes (chimpanzee)	CXXC1	XP_001154076.1
	CXXC1	XP_001093242.2
Danio rerio (zebrafish)	cxxc1l	NP_956893.1

Gene Ontology
Bibliography

Related articles in PubMed

CFP1 Regulates Histone H3K4 Trimethylation and Developmental Potential in Mouse Oocytes.
Yu C, Fan X, Sha QQ, Wang HH, Li BT, Dai XX, Shen L, Liu J, Wang L, Liu K, Tang F, Fan HY
Cell reports20(5)1161-1172(2017 Aug)

PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis.
Parvanov ED, Tian H, Billings T, Saxl RL, Spruce C, Aithal R, Krejci L, Paigen K, Petkov PM
Molecular biology of the cell28(3)488-499(2017 Feb)

Efficient differentiation of murine embryonic stem cells requires the binding of CXXC finger protein 1 to DNA or methylated histone H3-Lys4.
Mahadevan J, Skalnik DG
Gene594(1)1-9(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that CXXC finger protein 1 (Cfp1) is necessary for hematopoietic stem and multi-potential progenitor cell function and for the developmental potential of differentiating hematopoietic cells.
Title: The epigenetic regulator CXXC finger protein 1 is essential for murine hematopoiesis.

NF-kappaB recruitment enhanced the occupancy of the CpG island within the 14-3-3gamma promoter by CFP1.14-3-3gamma induction in CSR is enabled by the CFP1-mediated H3K4me3 enrichment in the promoter, dependent on NF-kappaB and sustained by E2A.
Title: Induction of activation-induced cytidine deaminase-targeting adaptor 14-3-3? is mediated by NF-?B-dependent recruitment of CFP1 to the 5'-CpG-3'-rich 14-3-3? promoter and is sustained by E2A.

The results demonstrated that Cfp1 is a specificity factor that integrates multiple signals, including promoter CpG content and gene activity, to regulate genome-wide patterns of H3K4me3.
Title: Cfp1 integrates both CpG content and gene activity for accurate H3K4me3 deposition in embryonic stem cells.

a primary function of non-methylated CpG islands is to genetically influence the local chromatin modification state by interaction with Cfp1 and perhaps other CpG-binding proteins
Title: CpG islands influence chromatin structure via the CpG-binding protein Cfp1.

embryonic stem cells lacking Cfp1 (CXXC1(-/-)) are viable but show increased levels of global histone H3K4 methylation, suggesting that Cfp1 functions to inhibit or restrict the activity of the Setd1A histone H3K4 methyltransferase complex.
Title: CXXC finger protein 1 restricts the Setd1A histone H3K4 methyltransferase complex to euchromatin.

The following Cxxc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cxxc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16313	NM_028868.3 Latest version!	Mus musculus CXXC finger 1 (PHD domain) (Cxxc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00
OMu54833	XM_006526302.3 Latest version!	Mus musculus CXXC finger 1 (PHD domain) (Cxxc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cxxc1 ( NM_028868.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083144.1 Mus musculus CXXC finger 1 (PHD domain) (Cxxc1), mRNA.

Price & Availability↑

CloneID	OMu16313
Clone ID Related Accession (Same CDS sequence)	NM_028868.3
Accession Version	NM_028868.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	CXXC-type zinc finger protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC133186.4. On Apr 30, 2007 this sequence version replaced NM_028868.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146688.1, AK083655.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGGAG ATGGCTCAGA CCTGGAACCT CCGGATGCCG GGGACGACAG CAAGTCTGAG 
AATGGGGAGA ACGCTCCCAT CTACTGCATC TGTCGCAAAC CGGACATCAA TTGCTTCATG 
ATTGGATGTG ACAACTGCAA CGAGTGGTTC CATGGAGACT GCATCCGGAT CACTGAGAAG 
ATGGCCAAGG CCATCCGGGA ATGGTACTGT CGGGAGTGCC GAGAGAAGGA CCCGAAGCTG 
GAGATTCGTT ACCGCCACAA AAAGTGCCGG GAGAGGGATG GCAGTGAGCG GGCCGGCAGT 
GAGCCCCGGG ATGAGGGAGG AGGGCGCAAG AGGCCGGCTT CAGATCCAGA GCTGCAGCGC 
CGGGCAGGGT CAGGGACAGG GGTTGGGGCC ATGCTTGCTC GGGGCTCTGC TTCCCCCCAC 
AAATCTTCTC CACAGCCCTT GGTGGCCACA CCTAGCCAGC ACCACCACCA ACAGCAGCAG 
CAGCAGCAGC AGCAAATCAA ACGATCAGCT CGGATGTGTG GTGAGTGCGA GGCCTGCCGA 
CGCACTGAGG ACTGTGGCCA CTGTGACTTC TGCCGTGACA TGAAGAAGTT TGGGGGCCCC 
AACAAGATCC GGCAGAAGTG CCGGCTTCGT CAGTGTCAGC TGCGGGCACG GGAATCGTAC 
AAGTACTTCC CTTCCTCGCT CTCGCCGGTG ACACCCTCAG AGGCCCTGCC AAGGCCCCGC 
CGGCCACCAC CCACTCAACA GCAGCCACAG CAGTCCCAGA AGCTGGGGCG TATTCGTGAA 
GATGAGGGGA CAGTGTTGTC ATCAGTGGTT AAGGAGCCAC CAGAGGCTAC AGCAACACCT 
GAGCCACTTT CAGATGAGGA CCTAGCACTG GACCCCGATC TGTACCAGGA CTTCTGTGCT 
GGGGCCTTTG ATGATCACGG CCTACCCTGG ATGAGCGATG CAGAAGAGTC CCCGTTCCTG 
GATCCTGCAC TGAGGAAGCG GGCGGTGAAG GTGAAGCACG TGAAGCGCCG GGAGAAGAAG 
TCCGAGAAGA AGAAGGAGGA GAGGTACAAA CGGCATCGAC AGAAGCAGAA ACATAAAGAC 
AAATGGAAAC ACCCAGAGAG GGCTGATGCC AAGGACCCTG CATCTCTCCC GCAGTGCCTG 
GGGCCTGGCT GTGTGCGGGC TGCCCAGCCT GGGTCCAAGT ATTGTTCAGA TGACTGTGGC 
ATGAAGCTGG CAGCCAACCG AATCTATGAG ATCCTCCCCC AGCGCATCCA GCAGTGGCAG 
CAAAGCCCCT GCATCGCTGA AGAGCACGGC AAGAAGCTAC TTGAGCGGAT CCGCCGTGAG 
CAGCAGAGCG CCCGCACCCG CCTTCAGGAA ATGGAGCGCA GATTCCACGA GCTTGAGGCC 
ATCATTCTTC GCGCTAAGCA GCAAGCTGTG CGAGAGGATG AGGAGAACAA CGAGAACGAC 
AGTGATGACA CAGATCTGCA GATCTTCTGC GTCTCCTGCG GGCATCCCAT CAACCCACGA 
GTTGCCTTGC GTCACATGGA ACGTTGCTAT GCTAAGTATG AGAGCCAGAC GTCTTTTGGG 
TCCATGTACC CCACACGCAT CGAGGGGGCT ACCCGACTCT TCTGTGATGT CTACAATCCT 
CAGAGCAAGA CATATTGTAA GCGGCTCCAG GTGCTGTGTC CTGAGCACTC CCGGGACCCC 
AAAGTGCCAG CTGATGAGGT CTGTGGGTGT CCACTTGTAC GTGATGTCTT TGAGCTCACA 
GGTGACTTCT GCCGCCTGCC CAAGCGCCAG TGTAATCGCC ATTATTGCTG GGAGAAGCTT 
CGGCGTGCAG AAGTGGACTT GGAGCGCGTG CGGGTGTGGT ACAAGCTGGA CGAGCTCTTT 
GAGCAGGAAC GTAATGTACG CACAGCCATG ACCAACAGAG CAGGGCTACT TGCCCTGATG 
CTTCACCAAA CGATCCAACA CGATCCACTC ACTACCGACC TTCGCTCTAG TGCCGACCGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083144.1
CDS	269..2251
Translation	MEGDGSDLEPPDAGDDSKSENGENAPIYCICRKPDINCFMIGCDNCNEWFHGDCIRITEKMAKAIREWYCRECREKDPKLEIRYRHKKCRERDGSERAGSEPRDEGGGRKRPASDPELQRRAGSGTGVGAMLARGSASPHKSSPQPLVATPSQHHHQQQQQQQQQIKRSARMCGECEACRRTEDCGHCDFCRDMKKFGGPNKIRQKCRLRQCQLRARESYKYFPSSLSPVTPSEALPRPRRPPPTQQQPQQSQKLGRIREDEGTVLSSVVKEPPEATATPEPLSDEDLALDPDLYQDFCAGAFDDHGLPWMSDAEESPFLDPALRKRAVKVKHVKRREKKSEKKKEERYKRHRQKQKHKDKWKHPERADAKDPASLPQCLGPGCVRAAQPGSKYCSDDCGMKLAANRIYEILPQRIQQWQQSPCIAEEHGKKLLERIRREQQSARTRLQEMERRFHELEAIILRAKQQAVREDEENNENDSDDTDLQIFCVSCGHPINPRVALRHMERCYAKYESQTSFGSMYPTRIEGATRLFCDVYNPQSKTYCKRLQVLCPEHSRDPKVPADEVCGCPLVRDVFELTGDFCRLPKRQCNRHYCWEKLRRAEVDLERVRVWYKLDELFEQERNVRTAMTNRAGLLALMLHQTIQHDPLTTDLRSSADR

Target ORF information:

RefSeq Version	NM_028868.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CXXC finger 1 (PHD domain) (Cxxc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028868.3

ORF Insert Sequence:

ATGGAAGGAG ATGGCTCAGA CCTGGAACCT CCGGATGCCG GGGACGACAG CAAGTCTGAG 
AATGGGGAGA ACGCTCCCAT CTACTGCATC TGTCGCAAAC CGGACATCAA TTGCTTCATG 
ATTGGATGTG ACAACTGCAA CGAGTGGTTC CATGGAGACT GCATCCGGAT CACTGAGAAG 
ATGGCCAAGG CCATCCGGGA ATGGTACTGT CGGGAGTGCC GAGAGAAGGA CCCGAAGCTG 
GAGATTCGTT ACCGCCACAA AAAGTGCCGG GAGAGGGATG GCAGTGAGCG GGCCGGCAGT 
GAGCCCCGGG ATGAGGGAGG AGGGCGCAAG AGGCCGGCTT CAGATCCAGA GCTGCAGCGC 
CGGGCAGGGT CAGGGACAGG GGTTGGGGCC ATGCTTGCTC GGGGCTCTGC TTCCCCCCAC 
AAATCTTCTC CACAGCCCTT GGTGGCCACA CCTAGCCAGC ACCACCACCA ACAGCAGCAG 
CAGCAGCAGC AGCAAATCAA ACGATCAGCT CGGATGTGTG GTGAGTGCGA GGCCTGCCGA 
CGCACTGAGG ACTGTGGCCA CTGTGACTTC TGCCGTGACA TGAAGAAGTT TGGGGGCCCC 
AACAAGATCC GGCAGAAGTG CCGGCTTCGT CAGTGTCAGC TGCGGGCACG GGAATCGTAC 
AAGTACTTCC CTTCCTCGCT CTCGCCGGTG ACACCCTCAG AGGCCCTGCC AAGGCCCCGC 
CGGCCACCAC CCACTCAACA GCAGCCACAG CAGTCCCAGA AGCTGGGGCG TATTCGTGAA 
GATGAGGGGA CAGTGTTGTC ATCAGTGGTT AAGGAGCCAC CAGAGGCTAC AGCAACACCT 
GAGCCACTTT CAGATGAGGA CCTAGCACTG GACCCCGATC TGTACCAGGA CTTCTGTGCT 
GGGGCCTTTG ATGATCACGG CCTACCCTGG ATGAGCGATG CAGAAGAGTC CCCGTTCCTG 
GATCCTGCAC TGAGGAAGCG GGCGGTGAAG GTGAAGCACG TGAAGCGCCG GGAGAAGAAG 
TCCGAGAAGA AGAAGGAGGA GAGGTACAAA CGGCATCGAC AGAAGCAGAA ACATAAAGAC 
AAATGGAAAC ACCCAGAGAG GGCTGATGCC AAGGACCCTG CATCTCTCCC GCAGTGCCTG 
GGGCCTGGCT GTGTGCGGGC TGCCCAGCCT GGGTCCAAGT ATTGTTCAGA TGACTGTGGC 
ATGAAGCTGG CAGCCAACCG AATCTATGAG ATCCTCCCCC AGCGCATCCA GCAGTGGCAG 
CAAAGCCCCT GCATCGCTGA AGAGCACGGC AAGAAGCTAC TTGAGCGGAT CCGCCGTGAG 
CAGCAGAGCG CCCGCACCCG CCTTCAGGAA ATGGAGCGCA GATTCCACGA GCTTGAGGCC 
ATCATTCTTC GCGCTAAGCA GCAAGCTGTG CGAGAGGATG AGGAGAACAA CGAGAACGAC 
AGTGATGACA CAGATCTGCA GATCTTCTGC GTCTCCTGCG GGCATCCCAT CAACCCACGA 
GTTGCCTTGC GTCACATGGA ACGTTGCTAT GCTAAGTATG AGAGCCAGAC GTCTTTTGGG 
TCCATGTACC CCACACGCAT CGAGGGGGCT ACCCGACTCT TCTGTGATGT CTACAATCCT 
CAGAGCAAGA CATATTGTAA GCGGCTCCAG GTGCTGTGTC CTGAGCACTC CCGGGACCCC 
AAAGTGCCAG CTGATGAGGT CTGTGGGTGT CCACTTGTAC GTGATGTCTT TGAGCTCACA 
GGTGACTTCT GCCGCCTGCC CAAGCGCCAG TGTAATCGCC ATTATTGCTG GGAGAAGCTT 
CGGCGTGCAG AAGTGGACTT GGAGCGCGTG CGGGTGTGGT ACAAGCTGGA CGAGCTCTTT 
GAGCAGGAAC GTAATGTACG CACAGCCATG ACCAACAGAG CAGGGCTACT TGCCCTGATG 
CTTCACCAAA CGATCCAACA CGATCCACTC ACTACCGACC TTCGCTCTAG TGCCGACCGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cxxc1 ( XM_006526302.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526365.1 Mus musculus CXXC finger 1 (PHD domain) (Cxxc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54833
Clone ID Related Accession (Same CDS sequence)	XM_006526302.3
Accession Version	XM_006526302.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	CXXC-type zinc finger protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526302.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGAG ATGGCTCAGA CCTGGAACCT CCGGATGCCG GGGACGACAG CAAGTCTGAG 
AATGGGGAGA ACGCTCCCAT CTACTGCATC TGTCGCAAAC CGGACATCAA TTGCTTCATG 
ATTGGATGTG ACAACTGCAA CGAGTGGTTC CATGGAGACT GCATCCGGAT CACTGAGAAG 
ATGGCCAAGG CCATCCGGGA ATGGTACTGT CGGGAGTGCC GAGAGAAGGA CCCGAAGCTG 
GAGATTCGTT ACCGCCACAA AAAGTGCCGG GAGAGGGATG GCAGTGAGCG GGCCGGCAGT 
GAGCCCCGGG ATGAGGGAGG AGGGCGCAAG AGGCCGGCTT CAGATCCAGA GCTGCAGCGC 
CGGGCAGGGT CAGGGACAGG GGTTGGGGCC ATGCTTGCTC GGGGCTCTGC TTCCCCCCAC 
AAATCTTCTC CACAGCCCTT GGTGGCCACA CCTAGCCAGC ACCACCACCA ACAGCAGCAG 
CAGCAGCAGC AGCAAATCAA ACGATCAGCT CGGATGTGTG GTGAGTGCGA GGCCTGCCGA 
CGCACTGAGG ACTGTGGCCA CTGTGACTTC TGCCGTGACA TGAAGAAGTT TGGGGGCCCC 
AACAAGATCC GGCAGAAGTG CCGGCTTCGT CAGTGTCAGC TGCGGGCACG GGAATCGTAC 
AAGTACTTCC CTTCCTCGCT CTCGCCGGTG ACACCCTCAG AGGCCCTGCC AAGGCCCCGC 
CGGCCACCAC CCACTCAACA GCAGCCACAG CAGTCCCAGA AGCTGGGGCG TATTCGTGAA 
GATGAGGGGA CAGTGTTGTC ATCAGTGGTT AAGGAGCCAC CAGAGGCTAC AGCAACACCT 
GAGCCACTTT CAGATGAGGA CCTAGCACTG GACCCCGATC TGTACCAGGA CTTCTGTGCT 
GGGGCCTTTG ATGATCACGG CCTACCCTGG ATGAGCGATG CAGAAGAGTC CCCGTTCCTG 
GATCCTGCAC TGAGGAAGCG GGCGGTGAAG GTGAAGCACG TGAAGCGCCG GGAGAAGAAG 
TCCGAGAAGA AGGTGATGGA GAGGAAGGAG GAGAGGTACA AACGGCATCG ACAGAAGCAG 
AAACATAAAG ACAAATGGAA ACACCCAGAG AGGGCTGATG CCAAGGACCC TGCATCTCTC 
CCGCAGTGCC TGGGGCCTGG CTGTGTGCGG GCTGCCCAGC CTGGGTCCAA GTATTGTTCA 
GATGACTGTG GCATGAAGCT GGCAGCCAAC CGAATCTATG AGATCCTCCC CCAGCGCATC 
CAGCAGTGGC AGCAAAGCCC CTGCATCGCT GAAGAGCACG GCAAGAAGCT ACTTGAGCGG 
ATCCGCCGTG AGCAGCAGAG CGCCCGCACC CGCCTTCAGG AAATGGAGCG CAGATTCCAC 
GAGCTTGAGG CCATCATTCT TCGCGCTAAG CAGCAAGCTG TGCGAGAGGA TGAGGAGAAC 
AACGAGAACG ACAGTGATGA CACAGATCTG CAGATCTTCT GCGTCTCCTG CGGGCATCCC 
ATCAACCCAC GAGTTGCCTT GCGTCACATG GAACGTTGCT ATGCTAAGTA TGAGAGCCAG 
ACGTCTTTTG GGTCCATGTA CCCCACACGC ATCGAGGGGG CTACCCGACT CTTCTGTGAT 
GTCTACAATC CTCAGAGCAA GACATATTGT AAGCGGCTCC AGGTGCTGTG TCCTGAGCAC 
TCCCGGGACC CCAAAGTGCC AGCTGATGAG GTCTGTGGGT GTCCACTTGT ACGTGATGTC 
TTTGAGCTCA CAGGTGACTT CTGCCGCCTG CCCAAGCGCC AGTGTAATCG CCATTATTGC 
TGGGAGAAGC TTCGGCGTGC AGAAGTGGAC TTGGAGCGCG TGCGGGTGTG GTACAAGCTG 
GACGAGCTCT TTGAGCAGGA ACGTAATGTA CGCACAGCCA TGACCAACAG AGCAGGGCTA 
CTTGCCCTGA TGCTTCACCA AACGATCCAA CACGATCCAC TCACTACCGA CCTTCGCTCT 
AGTGCCGACC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526365.1
CDS	389..2383
Translation	MEGDGSDLEPPDAGDDSKSENGENAPIYCICRKPDINCFMIGCDNCNEWFHGDCIRITEKMAKAIREWYCRECREKDPKLEIRYRHKKCRERDGSERAGSEPRDEGGGRKRPASDPELQRRAGSGTGVGAMLARGSASPHKSSPQPLVATPSQHHHQQQQQQQQQIKRSARMCGECEACRRTEDCGHCDFCRDMKKFGGPNKIRQKCRLRQCQLRARESYKYFPSSLSPVTPSEALPRPRRPPPTQQQPQQSQKLGRIREDEGTVLSSVVKEPPEATATPEPLSDEDLALDPDLYQDFCAGAFDDHGLPWMSDAEESPFLDPALRKRAVKVKHVKRREKKSEKKVMERKEERYKRHRQKQKHKDKWKHPERADAKDPASLPQCLGPGCVRAAQPGSKYCSDDCGMKLAANRIYEILPQRIQQWQQSPCIAEEHGKKLLERIRREQQSARTRLQEMERRFHELEAIILRAKQQAVREDEENNENDSDDTDLQIFCVSCGHPINPRVALRHMERCYAKYESQTSFGSMYPTRIEGATRLFCDVYNPQSKTYCKRLQVLCPEHSRDPKVPADEVCGCPLVRDVFELTGDFCRLPKRQCNRHYCWEKLRRAEVDLERVRVWYKLDELFEQERNVRTAMTNRAGLLALMLHQTIQHDPLTTDLRSSADR

Target ORF information:

RefSeq Version	XM_006526302.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CXXC finger 1 (PHD domain) (Cxxc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526302.3

ORF Insert Sequence:

ATGGAAGGAG ATGGCTCAGA CCTGGAACCT CCGGATGCCG GGGACGACAG CAAGTCTGAG 
AATGGGGAGA ACGCTCCCAT CTACTGCATC TGTCGCAAAC CGGACATCAA TTGCTTCATG 
ATTGGATGTG ACAACTGCAA CGAGTGGTTC CATGGAGACT GCATCCGGAT CACTGAGAAG 
ATGGCCAAGG CCATCCGGGA ATGGTACTGT CGGGAGTGCC GAGAGAAGGA CCCGAAGCTG 
GAGATTCGTT ACCGCCACAA AAAGTGCCGG GAGAGGGATG GCAGTGAGCG GGCCGGCAGT 
GAGCCCCGGG ATGAGGGAGG AGGGCGCAAG AGGCCGGCTT CAGATCCAGA GCTGCAGCGC 
CGGGCAGGGT CAGGGACAGG GGTTGGGGCC ATGCTTGCTC GGGGCTCTGC TTCCCCCCAC 
AAATCTTCTC CACAGCCCTT GGTGGCCACA CCTAGCCAGC ACCACCACCA ACAGCAGCAG 
CAGCAGCAGC AGCAAATCAA ACGATCAGCT CGGATGTGTG GTGAGTGCGA GGCCTGCCGA 
CGCACTGAGG ACTGTGGCCA CTGTGACTTC TGCCGTGACA TGAAGAAGTT TGGGGGCCCC 
AACAAGATCC GGCAGAAGTG CCGGCTTCGT CAGTGTCAGC TGCGGGCACG GGAATCGTAC 
AAGTACTTCC CTTCCTCGCT CTCGCCGGTG ACACCCTCAG AGGCCCTGCC AAGGCCCCGC 
CGGCCACCAC CCACTCAACA GCAGCCACAG CAGTCCCAGA AGCTGGGGCG TATTCGTGAA 
GATGAGGGGA CAGTGTTGTC ATCAGTGGTT AAGGAGCCAC CAGAGGCTAC AGCAACACCT 
GAGCCACTTT CAGATGAGGA CCTAGCACTG GACCCCGATC TGTACCAGGA CTTCTGTGCT 
GGGGCCTTTG ATGATCACGG CCTACCCTGG ATGAGCGATG CAGAAGAGTC CCCGTTCCTG 
GATCCTGCAC TGAGGAAGCG GGCGGTGAAG GTGAAGCACG TGAAGCGCCG GGAGAAGAAG 
TCCGAGAAGA AGGTGATGGA GAGGAAGGAG GAGAGGTACA AACGGCATCG ACAGAAGCAG 
AAACATAAAG ACAAATGGAA ACACCCAGAG AGGGCTGATG CCAAGGACCC TGCATCTCTC 
CCGCAGTGCC TGGGGCCTGG CTGTGTGCGG GCTGCCCAGC CTGGGTCCAA GTATTGTTCA 
GATGACTGTG GCATGAAGCT GGCAGCCAAC CGAATCTATG AGATCCTCCC CCAGCGCATC 
CAGCAGTGGC AGCAAAGCCC CTGCATCGCT GAAGAGCACG GCAAGAAGCT ACTTGAGCGG 
ATCCGCCGTG AGCAGCAGAG CGCCCGCACC CGCCTTCAGG AAATGGAGCG CAGATTCCAC 
GAGCTTGAGG CCATCATTCT TCGCGCTAAG CAGCAAGCTG TGCGAGAGGA TGAGGAGAAC 
AACGAGAACG ACAGTGATGA CACAGATCTG CAGATCTTCT GCGTCTCCTG CGGGCATCCC 
ATCAACCCAC GAGTTGCCTT GCGTCACATG GAACGTTGCT ATGCTAAGTA TGAGAGCCAG 
ACGTCTTTTG GGTCCATGTA CCCCACACGC ATCGAGGGGG CTACCCGACT CTTCTGTGAT 
GTCTACAATC CTCAGAGCAA GACATATTGT AAGCGGCTCC AGGTGCTGTG TCCTGAGCAC 
TCCCGGGACC CCAAAGTGCC AGCTGATGAG GTCTGTGGGT GTCCACTTGT ACGTGATGTC 
TTTGAGCTCA CAGGTGACTT CTGCCGCCTG CCCAAGCGCC AGTGTAATCG CCATTATTGC 
TGGGAGAAGC TTCGGCGTGC AGAAGTGGAC TTGGAGCGCG TGCGGGTGTG GTACAAGCTG 
GACGAGCTCT TTGAGCAGGA ACGTAATGTA CGCACAGCCA TGACCAACAG AGCAGGGCTA 
CTTGCCCTGA TGCTTCACCA AACGATCCAA CACGATCCAC TCACTACCGA CCTTCGCTCT 
AGTGCCGACC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CFP1 Regulates Histone H3K4 Trimethylation and Developmental Potential in Mouse Oocytes.
Cell reports20(5)1161-1172(2017 Aug)
Yu C,Fan X,Sha QQ,Wang HH,Li BT,Dai XX,Shen L,Liu J,Wang L,Liu K,Tang F,Fan HY

PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis.
Molecular biology of the cell28(3)488-499(2017 Feb)
Parvanov ED,Tian H,Billings T,Saxl RL,Spruce C,Aithal R,Krejci L,Paigen K,Petkov PM

Efficient differentiation of murine embryonic stem cells requires the binding of CXXC finger protein 1 to DNA or methylated histone H3-Lys4.
Gene594(1)1-9(2016 Dec)
Mahadevan J,Skalnik DG

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Cxxc1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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