Cda cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cda
Entrez Gene ID	72269
Full Name	cytidine deaminase
Synonyms	2210401N16Rik
General protein information	Preferred Names cytidine deaminase Names cytidine deaminase cytidine aminohydrolase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_028176.1	NP_082452.1	cytidine deaminase
XM_006539196.3	XP_006539259.1	cytidine deaminase isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CDA	XP_001161389.1
Rattus norvegicus (Norway rat)	LOC100909857	XP_003750114.1
Danio rerio (zebrafish)	LOC559958	XP_001923622.2
Schizosaccharomyces pombe (fission yeast)	cdd1	NP_594321.1
	CDA	XP_001096632.1
Canis lupus familiaris (dog)	CDA	XP_544519.1
Rattus norvegicus (Norway rat)	Cda	NP_001102158.1
Gallus gallus (chicken)	CDA	NP_001139516.1
Saccharomyces cerevisiae (baker's yeast)	CDD1	NP_013346.1
Neurospora crassa	NCU08359	XP_965262.1
Bos taurus (cattle)	CDA	NP_001180039.1
Eremothecium gossypii	AGOS_AGL123W	NP_986544.1
Mus musculus (house mouse)	Cda	NP_082452.1
Kluyveromyces lactis	KLLA0D18414g	XP_453876.1
Magnaporthe oryzae (rice blast fungus)	MGG_14549	XP_003710043.1
Xenopus tropicalis (tropical clawed frog)	cda	NP_001017217.1
Homo sapiens (human)	CDA	NP_001776.1
Danio rerio (zebrafish)	cda	NP_991242.1

Gene Ontology
Bibliography

Related articles in PubMed

Combined treatment, based on lysomustine administration with mesenchymal stem cells expressing cytosine deaminase therapy, leads to pronounced murine Lewis lung carcinoma growth inhibition.
Krassikova LS, Karshieva SS, Cheglakov IB, Belyavsky AV
The journal of gene medicine18(9)220-33(2016 Sep)

Targeting activation-induced cytidine deaminase prevents colon cancer development despite persistent colonic inflammation.
Takai A, Marusawa H, Minaki Y, Watanabe T, Nakase H, Kinoshita K, Tsujimoto G, Chiba T
Oncogene31(13)1733-42(2012 Mar)

A novel mouse model of podocyte depletion.
Wang L, Tang Y, Howell DN, Ruiz P, Spurney RF
Nephron. Experimental nephrology121(1-2)e10-22(2012)

Activation-induced cytidine deaminase deficiency causes organ-specific autoimmune disease.
Hase K, Takahashi D, Ebisawa M, Kawano S, Itoh K, Ohno H
PloS one3(8)e3033(2008 Aug)

Identification of transcripts with enriched expression in the developing and adult pancreas.
Hoffman BG, Zavaglia B, Witzsche J, Ruiz de Algara T, Beach M, Hoodless PA, Jones SJ, Marra MA, Helgason CD
Genome biology9(6)R99(2008)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Activation-induced cytidine deaminase plays an important role in the containment of autoimmune diseases by negative regulation of autoreactive B cells.
Title: Activation-induced cytidine deaminase deficiency causes organ-specific autoimmune disease.

a drug-resistance gene highly suitable for myeloprotective purposes, which might best be used in combination with selectable drug-resistance genes.
Title: Gene transfer of cytidine deaminase protects myelopoiesis from cytidine analogs in an in vivo murine transplant model.

On the basis of known structures, structural analysis further reveals two subclasses of homotetrameric CDAs that can be identified according to the position of the charge-neutralizing arginine residue.
Title: The 1.48 A resolution crystal structure of the homotetrameric cytidine deaminase from mouse.

The following Cda gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cda cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu34446	XM_006539196.3 Latest version!	Mus musculus cytidine deaminase (Cda), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu05256	NM_028176.1 Latest version!	Mus musculus cytidine deaminase (Cda), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cda ( XM_006539196.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539259.1 Mus musculus cytidine deaminase (Cda), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34446
Clone ID Related Accession (Same CDS sequence)	XM_006539196.3
Accession Version	XM_006539196.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cytidine deaminase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539196.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGG AACGCCCCTC CTGTGCTGTG GAGCCCGAGC ACGTCCAGCG GCTGCTGCTG 
TCCTCTCGTG AGGCCAAGAA GTCAGCCTAC TGCCCCTACA GCCGCTTCCC TGTGGGGGCT 
GCCCTGCTCA CCGGGGACGG GAGGATCTTC TCTGCACTGG GCAAGCAATC TGCGTCTTCA 
TACCAGGCGA GCACTCTGCC ACAACCACTT TATCAAGCCT CAGCAGCATC TCCCTTCACC 
TTCACCTTCA CCTTGACCTT GACCTTAGGG ATGATGCAGC TGCTCCTCTC TGCTTCCAGG 
TTTGGGAACC AGGATGAGGC ATTAGAGAAA CAAAGACCCG AACATTCTTG GAAAATCCAC 
GCCACAGCAG GCCCCAGTGT TCTCCAGTAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539259.1
CDS	369..764
Translation	MAQERPSCAVEPEHVQRLLLSSREAKKSAYCPYSRFPVGAALLTGDGRIFSALGKQSASSYQASTLPQPLYQASAASPFTFTFTLTLTLGMMQLLLSASRFGNQDEALEKQRPEHSWKIHATAGPSVLQYF

Target ORF information:

RefSeq Version	XM_006539196.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytidine deaminase (Cda), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539196.3

ORF Insert Sequence:

ATGGCCCAGG AACGCCCCTC CTGTGCTGTG GAGCCCGAGC ACGTCCAGCG GCTGCTGCTG 
TCCTCTCGTG AGGCCAAGAA GTCAGCCTAC TGCCCCTACA GCCGCTTCCC TGTGGGGGCT 
GCCCTGCTCA CCGGGGACGG GAGGATCTTC TCTGCACTGG GCAAGCAATC TGCGTCTTCA 
TACCAGGCGA GCACTCTGCC ACAACCACTT TATCAAGCCT CAGCAGCATC TCCCTTCACC 
TTCACCTTCA CCTTGACCTT GACCTTAGGG ATGATGCAGC TGCTCCTCTC TGCTTCCAGG 
TTTGGGAACC AGGATGAGGC ATTAGAGAAA CAAAGACCCG AACATTCTTG GAAAATCCAC 
GCCACAGCAG GCCCCAGTGT TCTCCAGTAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cda ( NM_028176.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082452.1 Mus musculus cytidine deaminase (Cda), mRNA.

Price & Availability↑

CloneID	OMu05256
Clone ID Related Accession (Same CDS sequence)	NM_028176.1
Accession Version	NM_028176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	cytidine deaminase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC050114.1. On Jan 24, 2005 this sequence version replaced XM_131801.2. ##Evidence-Data-START## Transcript exon combination :: BC050114.1, AK008793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCAGG AACGCCCCTC CTGTGCTGTG GAGCCCGAGC ACGTCCAGCG GCTGCTGCTG 
TCCTCTCGTG AGGCCAAGAA GTCAGCCTAC TGCCCCTACA GCCGCTTCCC TGTGGGGGCT 
GCCCTGCTCA CCGGGGACGG GAGGATCTTC TCTGGGTGCA ACATAGAAAA CGCCTGCTAC 
CCACTAGGTG TGTGTGCCGA GCGGACTGCC ATCCAGAAGG CCATCTCCGA AGGGTACAAG 
GATTTCAGGG CTATTGCCAT CTCTAGTGAC TTGCAAGAAG AGTTCATCTC TCCCTGTGGA 
GCCTGCCGAC AAGTCATGAG AGAGTTTGGC ACCGACTGGG CCGTGTACAT GACCAAGCCG 
GATGGCACAT TCGTAGTCAG GACGGTCCAG GAGCTGCTGC CGGCCTCGTT TGGACCTGAA 
GACCTGCAGA AGATTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082452.1
CDS	13..453
Translation	MAQERPSCAVEPEHVQRLLLSSREAKKSAYCPYSRFPVGAALLTGDGRIFSGCNIENACYPLGVCAERTAIQKAISEGYKDFRAIAISSDLQEEFISPCGACRQVMREFGTDWAVYMTKPDGTFVVRTVQELLPASFGPEDLQKIQ

Target ORF information:

RefSeq Version	NM_028176.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytidine deaminase (Cda), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028176.1

ORF Insert Sequence:

ATGGCCCAGG AACGCCCCTC CTGTGCTGTG GAGCCCGAGC ACGTCCAGCG GCTGCTGCTG 
TCCTCTCGTG AGGCCAAGAA GTCAGCCTAC TGCCCCTACA GCCGCTTCCC TGTGGGGGCT 
GCCCTGCTCA CCGGGGACGG GAGGATCTTC TCTGGGTGCA ACATAGAAAA CGCCTGCTAC 
CCACTAGGTG TGTGTGCCGA GCGGACTGCC ATCCAGAAGG CCATCTCCGA AGGGTACAAG 
GATTTCAGGG CTATTGCCAT CTCTAGTGAC TTGCAAGAAG AGTTCATCTC TCCCTGTGGA 
GCCTGCCGAC AAGTCATGAG AGAGTTTGGC ACCGACTGGG CCGTGTACAT GACCAAGCCG 
GATGGCACAT TCGTAGTCAG GACGGTCCAG GAGCTGCTGC CGGCCTCGTT TGGACCTGAA 
GACCTGCAGA AGATTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Combined treatment, based on lysomustine administration with mesenchymal stem cells expressing cytosine deaminase therapy, leads to pronounced murine Lewis lung carcinoma growth inhibition.
The journal of gene medicine18(9)220-33(2016 Sep)
Krassikova LS,Karshieva SS,Cheglakov IB,Belyavsky AV

Targeting activation-induced cytidine deaminase prevents colon cancer development despite persistent colonic inflammation.
Oncogene31(13)1733-42(2012 Mar)
Takai A,Marusawa H,Minaki Y,Watanabe T,Nakase H,Kinoshita K,Tsujimoto G,Chiba T

A novel mouse model of podocyte depletion.
Nephron. Experimental nephrology121(1-2)e10-22(2012)
Wang L,Tang Y,Howell DN,Ruiz P,Spurney RF

Activation-induced cytidine deaminase deficiency causes organ-specific autoimmune disease.
PloS one3(8)e3033(2008 Aug)
Hase K,Takahashi D,Ebisawa M,Kawano S,Itoh K,Ohno H

Identification of transcripts with enriched expression in the developing and adult pancreas.
Genome biology9(6)R99(2008)
Hoffman BG,Zavaglia B,Witzsche J,Ruiz de Algara T,Beach M,Hoodless PA,Jones SJ,Marra MA,Helgason CD