Gtpbp4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gtpbp4
Entrez Gene ID	69237
Full Name	GTP binding protein 4
Synonyms	2610028C09Rik,Crfg,Gtpbp3,NGB,Nog1
General protein information	Preferred Names GTP binding protein 4 Names nucleolar GTP-binding protein 1 GTP binding protein 3 GTP-binding protein NGB Nucleolar GTP-binding protein 1 (Chronic renal failure gene protein) (GTP-binding protein NGB) chronic renal failure gene protein chronic renal failure protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017315592.1	XP_017171081.1	nucleolar GTP-binding protein 1 isoform X1
NM_027000.4	NP_081276.2	nucleolar GTP-binding protein 1
XM_017315592.2	XP_017171081.1	nucleolar GTP-binding protein 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GTPBP4	XP_535203.3
Bos taurus (cattle)	GTPBP4	NP_001179530.1
Rattus norvegicus (Norway rat)	Gtpbp4	NP_446141.2
Danio rerio (zebrafish)	gtpbp4	NP_956145.1
Drosophila melanogaster (fruit fly)	CG8801	NP_610484.1
Saccharomyces cerevisiae (baker's yeast)	NOG1	NP_015232.1
Arabidopsis thaliana (thale cress)	AT1G10300	NP_172501.1
	GTPBP4	XP_002805585.1
Caenorhabditis elegans (roundworm)	T07A9.9	NP_741288.1
Kluyveromyces lactis	KLLA0E24135g	XP_455039.1
Oryza sativa (rice)	Os06g0195900	NP_001057050.1
Pan troglodytes (chimpanzee)	GTPBP4	XP_003312478.1
Magnaporthe oryzae (rice blast fungus)	MGG_07136	XP_003715331.1
Neurospora crassa	NCU05289	XP_962060.2
Oryza sativa (rice)	Os07g0110200	NP_001058730.2
Xenopus tropicalis (tropical clawed frog)	gtpbp4	XP_002935223.2
Mus musculus (house mouse)	Gtpbp4	NP_081276.2
Gallus gallus (chicken)	GTPBP4	NP_001006354.1
Arabidopsis thaliana (thale cress)	AT1G50920	NP_175505.1
Homo sapiens (human)	GTPBP4	NP_036473.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007050	XP_308713.2
Eremothecium gossypii	AGOS_AGR194W	NP_986860.1
Schizosaccharomyces pombe (fission yeast)	nog1	XP_001713127.1

Gene Ontology
Bibliography

Related articles in PubMed

Two orthogonal cleavages separate subunit RNAs in mouse ribosome biogenesis.
Wang M, Anikin L, Pestov DG
Nucleic acids research42(17)11180-91(2014)

Restricting conformational flexibility of the switch II region creates a dominant-inhibitory phenotype in Obg GTPase Nog1.
Lapik YR, Misra JM, Lau LF, Pestov DG
Molecular and cellular biology27(21)7735-44(2007 Nov)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

The heme environment of mouse neuroglobin. Evidence for the presence of two conformations of the heme pocket.
Couture M, Burmester T, Hankeln T, Rousseau DL
The Journal of biological chemistry276(39)36377-82(2001 Sep)

Identification of a novel nuclear guanosine triphosphate-binding protein differentially expressed in renal disease.
Laping NJ, Olson BA, Zhu Y
Journal of the American Society of Nephrology : JASN12(5)883-90(2001 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that conformational changes in the switch II element of Nog1 have a critical importance for the dissociation of preribosome-bound factors during intranucleolar maturation.
Title: Restricting conformational flexibility of the switch II region creates a dominant-inhibitory phenotype in Obg GTPase Nog1.

Resonance Raman spectroscopy was used to determine the structure of the heme environment in Ngb from mice.
Title: The heme environment of mouse neuroglobin. Evidence for the presence of two conformations of the heme pocket.

The following Gtpbp4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gtpbp4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03397	NM_027000.4 Latest version!	Mus musculus GTP binding protein 4 (Gtpbp4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu78514	XM_017315592.2 Latest version!	Mus musculus GTP binding protein 4 (Gtpbp4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu78514	XM_017315592.1	Mus musculus GTP binding protein 4 (Gtpbp4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gtpbp4 ( NM_027000.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081276.2 Mus musculus GTP binding protein 4 (Gtpbp4), mRNA.

Price & Availability↑

CloneID	OMu03397
Clone ID Related Accession (Same CDS sequence)	NM_027000.4
Accession Version	NM_027000.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	nucleolar GTP-binding protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC125484.4 and AC124732.5. On Apr 30, 2007 this sequence version replaced NM_027000.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC025431.1, AK166711.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCATT ACAACTTCAA GAAGATTACG GTGGTGCCGT CTGCCAAGGA CTTTATTGAC 
CTAACATTAT CCAAGACTCA ACGAAAGACT CCAACGGTTA TTCATAAACA TTATCAAATT 
CATCGGATTC GACATTTTTA TATGAGAAAA GTCAAATTTA CTCAACAGAA TTACCATGAC 
AGACTGTCAC AGATTCTGTC GGATTTCCCC AAATTGGATG ATATCCATCC GTTTTATGCT 
GACTTGATGA ATATTCTCTA TGACAAGGAT CACTACAAGT TGGCTCTAGG TCAAATAAAT 
ATTGCCAAAA ACTTGGTGGA CAATGTTGCT AAAGATTATG TGCGTCTTAT GAAATATGGT 
GATTCTCTGT ACCGATGCAA GCAGCTGAAG CGCGCGGCCC TTGGACGAAT GTGTACTATT 
ATCAAGAGAC AGAGGCAGAG CTTGGAGTAT CTGGAACAAG TACGTCAACA TTTATCCCGC 
TTGCCAACCA TTGATCCAAA TACAAGGACT CTGCTTTTGT GTGGGTATCC AAATGTTGGG 
AAATCCAGCT TCATCAACAA GGTGACAAGA GCAGATGTGG ACGTTCAACC CTATGCATTT 
ACCACCAAAT CTCTGTTTGT TGGGCACATG GATTATAAGT ATCTGCGCTG GCAGGTTGTA 
GATACCCCAG GGATTTTGGA TCATCCTTTG GAGGATCGGA ACACCATTGA GATGCAGGCC 
ATCACAGCCC TGGCCCACCT CCGAGCTGCA GTTCTGTATG TGATGGATCT GTCTGAGCAG 
TGTGGACATG GGTTAAAGGA GCAGTTAGAA CTCTTCCAGA ATATCAGACC TCTGTTTATC 
AACAAGCCTC TTATTGTTGT AGCAAACAAA TGTGATGTGA AGAGAATAGC TGAACTTTCT 
GAAGAAGATC AGAAAATATT TCTAGACTTG CAGGCTGAAG GATTCCCTGT CATCGAGACC 
AGTACCCTGA CTGAGGAAGG TGTCATTCAA GTTAAAACAG AGGCGTGTGA CAGGCTTTTG 
GCTCACCGAG TAGAAACGAA AATGAAAGGA AATAAAGTGA ATGAGGTTCT GAACAGATTG 
CATTTAGCTG TGCCCAACAA GAGAGATGAT AAGGAGAGAC CCCCTTTCAT CCCAGAAGGA 
GTGATAGCTC GAAGGAAGAG AATGGAGATT GAAGAGCCCA AGAAGAAGAG GGAGCGAGAT 
CTTGAGCTGG AAATGGGAGA TGATTACATT TTGGATCTTC AGAAATACTG GGACTTAATG 
AATTCATCTG AGAAATATGA TAAGATACCA GAAATTTGGG AAGGCCACAA TGTTGCTGAT 
TATATTGATC CTGCTATCAT GAAGAAATTA GAAGAGTTGG AAAAGGAAGA AGAGCTAAGA 
ACAGCTGCTG GGGAGTATGA CAGTGACTCT GAGAGTGAAG ACGAGGAAAT GATGGAAATC 
CGACAGCTGG CAAAGCAAAT TAGAGAAAAG AAGAAGTTGA AAATTCTACA GTCCAAAGAA 
AAGAATAAAC AGGGACCCAG GATGCCCCGA ACTGCCAAGA AGGTTCAACG GGCAGATTTA 
GAGAATGAGA TGCGTAGTCT TGGTGTGGAC ATGGATGATA AAAACAATGC CCACTATGCA 
GTCCAGGCAA GAAGGTCTCG GAGTGTCACG AGGAAAAGAA AACGAGAAGA ATCTGTTCCA 
CCCTCTTCCA CAGCGCGGAG TCGCAGTTGT TCTAGAACTC CACGTGATGT TTCTGGTCTT 
CGGGATGTCA AGATGGTGAA GAAAGCCAAG ACTATGATGA AGAAAGCTCA GAAGAAGATG 
AATCGCTTGG GGAAGAAAGG GGAAGCTGAT AGGCATGTGT TTGATATGAA GCCCAAGCAC 
TTGCTGTCTG GGAAGAGGAA AGCTGGTAAA AAGGACAGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081276.2
CDS	17..1921
Translation	MAHYNFKKITVVPSAKDFIDLTLSKTQRKTPTVIHKHYQIHRIRHFYMRKVKFTQQNYHDRLSQILSDFPKLDDIHPFYADLMNILYDKDHYKLALGQINIAKNLVDNVAKDYVRLMKYGDSLYRCKQLKRAALGRMCTIIKRQRQSLEYLEQVRQHLSRLPTIDPNTRTLLLCGYPNVGKSSFINKVTRADVDVQPYAFTTKSLFVGHMDYKYLRWQVVDTPGILDHPLEDRNTIEMQAITALAHLRAAVLYVMDLSEQCGHGLKEQLELFQNIRPLFINKPLIVVANKCDVKRIAELSEEDQKIFLDLQAEGFPVIETSTLTEEGVIQVKTEACDRLLAHRVETKMKGNKVNEVLNRLHLAVPNKRDDKERPPFIPEGVIARRKRMEIEEPKKKRERDLELEMGDDYILDLQKYWDLMNSSEKYDKIPEIWEGHNVADYIDPAIMKKLEELEKEEELRTAAGEYDSDSESEDEEMMEIRQLAKQIREKKKLKILQSKEKNKQGPRMPRTAKKVQRADLENEMRSLGVDMDDKNNAHYAVQARRSRSVTRKRKREESVPPSSTARSRSCSRTPRDVSGLRDVKMVKKAKTMMKKAQKKMNRLGKKGEADRHVFDMKPKHLLSGKRKAGKKDRR

Target ORF information:

RefSeq Version	NM_027000.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTP binding protein 4 (Gtpbp4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027000.4

ORF Insert Sequence:

ATGGCGCATT ACAACTTCAA GAAGATTACG GTGGTGCCGT CTGCCAAGGA CTTTATTGAC 
CTAACATTAT CCAAGACTCA ACGAAAGACT CCAACGGTTA TTCATAAACA TTATCAAATT 
CATCGGATTC GACATTTTTA TATGAGAAAA GTCAAATTTA CTCAACAGAA TTACCATGAC 
AGACTGTCAC AGATTCTGTC GGATTTCCCC AAATTGGATG ATATCCATCC GTTTTATGCT 
GACTTGATGA ATATTCTCTA TGACAAGGAT CACTACAAGT TGGCTCTAGG TCAAATAAAT 
ATTGCCAAAA ACTTGGTGGA CAATGTTGCT AAAGATTATG TGCGTCTTAT GAAATATGGT 
GATTCTCTGT ACCGATGCAA GCAGCTGAAG CGCGCGGCCC TTGGACGAAT GTGTACTATT 
ATCAAGAGAC AGAGGCAGAG CTTGGAGTAT CTGGAACAAG TACGTCAACA TTTATCCCGC 
TTGCCAACCA TTGATCCAAA TACAAGGACT CTGCTTTTGT GTGGGTATCC AAATGTTGGG 
AAATCCAGCT TCATCAACAA GGTGACAAGA GCAGATGTGG ACGTTCAACC CTATGCATTT 
ACCACCAAAT CTCTGTTTGT TGGGCACATG GATTATAAGT ATCTGCGCTG GCAGGTTGTA 
GATACCCCAG GGATTTTGGA TCATCCTTTG GAGGATCGGA ACACCATTGA GATGCAGGCC 
ATCACAGCCC TGGCCCACCT CCGAGCTGCA GTTCTGTATG TGATGGATCT GTCTGAGCAG 
TGTGGACATG GGTTAAAGGA GCAGTTAGAA CTCTTCCAGA ATATCAGACC TCTGTTTATC 
AACAAGCCTC TTATTGTTGT AGCAAACAAA TGTGATGTGA AGAGAATAGC TGAACTTTCT 
GAAGAAGATC AGAAAATATT TCTAGACTTG CAGGCTGAAG GATTCCCTGT CATCGAGACC 
AGTACCCTGA CTGAGGAAGG TGTCATTCAA GTTAAAACAG AGGCGTGTGA CAGGCTTTTG 
GCTCACCGAG TAGAAACGAA AATGAAAGGA AATAAAGTGA ATGAGGTTCT GAACAGATTG 
CATTTAGCTG TGCCCAACAA GAGAGATGAT AAGGAGAGAC CCCCTTTCAT CCCAGAAGGA 
GTGATAGCTC GAAGGAAGAG AATGGAGATT GAAGAGCCCA AGAAGAAGAG GGAGCGAGAT 
CTTGAGCTGG AAATGGGAGA TGATTACATT TTGGATCTTC AGAAATACTG GGACTTAATG 
AATTCATCTG AGAAATATGA TAAGATACCA GAAATTTGGG AAGGCCACAA TGTTGCTGAT 
TATATTGATC CTGCTATCAT GAAGAAATTA GAAGAGTTGG AAAAGGAAGA AGAGCTAAGA 
ACAGCTGCTG GGGAGTATGA CAGTGACTCT GAGAGTGAAG ACGAGGAAAT GATGGAAATC 
CGACAGCTGG CAAAGCAAAT TAGAGAAAAG AAGAAGTTGA AAATTCTACA GTCCAAAGAA 
AAGAATAAAC AGGGACCCAG GATGCCCCGA ACTGCCAAGA AGGTTCAACG GGCAGATTTA 
GAGAATGAGA TGCGTAGTCT TGGTGTGGAC ATGGATGATA AAAACAATGC CCACTATGCA 
GTCCAGGCAA GAAGGTCTCG GAGTGTCACG AGGAAAAGAA AACGAGAAGA ATCTGTTCCA 
CCCTCTTCCA CAGCGCGGAG TCGCAGTTGT TCTAGAACTC CACGTGATGT TTCTGGTCTT 
CGGGATGTCA AGATGGTGAA GAAAGCCAAG ACTATGATGA AGAAAGCTCA GAAGAAGATG 
AATCGCTTGG GGAAGAAAGG GGAAGCTGAT AGGCATGTGT TTGATATGAA GCCCAAGCAC 
TTGCTGTCTG GGAAGAGGAA AGCTGGTAAA AAGGACAGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gtpbp4 ( XM_017315592.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171081.1 Mus musculus GTP binding protein 4 (Gtpbp4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu78514
Clone ID Related Accession (Same CDS sequence)	XM_017315592.1 , XM_017315592.2
Accession Version	XM_017315592.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nucleolar GTP-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCATT ACAACTTCAA GAAGATTACG GTGGTGCCGT CTGCCAAGGA TATCCATCCG 
TTTTATGCTG ACTTGATGAA TATTCTCTAT GACAAGGATC ACTACAAGTT GGCTCTAGGT 
CAAATAAATA TTGCCAAAAA CTTGGTGGAC AATGTTGCTA AAGATTATGT GCGTCTTATG 
AAATATGGTG ATTCTCTGTA CCGATGCAAG CAGCTGAAGC GCGCGGCCCT TGGACGAATG 
TGTACTATTA TCAAGAGACA GAGGCAGAGC TTGGAGTATC TGGAACAAGT ACGTCAACAT 
TTATCCCGCT TGCCAACCAT TGATCCAAAT ACAAGGACTC TGCTTTTGTG TGGGTATCCA 
AATGTTGGGA AATCCAGCTT CATCAACAAG GTGACAAGAG CAGATGTGGA CGTTCAACCC 
TATGCATTTA CCACCAAATC TCTGTTTGTT GGGCACATGG ATTATAAGTA TCTGCGCTGG 
CAGGTTGTAG ATACCCCAGG GATTTTGGAT CATCCTTTGG AGGATCGGAA CACCATTGAG 
ATGCAGGCCA TCACAGCCCT GGCCCACCTC CGAGCTGCAG TTCTGTATGT GATGGATCTG 
TCTGAGCAGT GTGGACATGG GTTAAAGGAG CAGTTAGAAC TCTTCCAGAA TATCAGACCT 
CTGTTTATCA ACAAGCCTCT TATTGTTGTA GCAAACAAAT GTGATGTGAA GAGAATAGCT 
GAACTTTCTG AAGAAGATCA GAAAATATTT CTAGACTTGC AGGCTGAAGG ATTCCCTGTC 
ATCGAGACCA GTACCCTGAC TGAGGAAGGT GTCATTCAAG TTAAAACAGA GGCGTGTGAC 
AGGCTTTTGG CTCACCGAGT AGAAACGAAA ATGAAAGGAA ATAAAGTGAA TGAGGTTCTG 
AACAGATTGC ATTTAGCTGT GCCCAACAAG AGAGATGATA AGGAGAGACC CCCTTTCATC 
CCAGAAGGAG TGATAGCTCG AAGGAAGAGA ATGGAGATTG AAGAGCCCAA GAAGAAGAGG 
GAGCGAGATC TTGAGCTGGA AATGGGAGAT GATTACATTT TGGATCTTCA GAAATACTGG 
GACTTAATGA ATTCATCTGA GAAATATGAT AAGATACCAG AAATTTGGGA AGGCCACAAT 
GTTGCTGATT ATATTGATCC TGCTATCATG AAGAAATTAG AAGAGTTGGA AAAGGAAGAA 
GAGCTAAGAA CAGCTGCTGG GGAGTATGAC AGTGACTCTG AGAGTGAAGA CGAGGAAATG 
ATGGAAATCC GACAGCTGGC AAAGCAAATT AGAGAAAAGA AGAAGTTGAA AATTCTACAG 
TCCAAAGAAA AGAATAAACA GGGACCCAGG ATGCCCCGAA CTGCCAAGAA GGTTCAACGG 
GCAGATTTAG AGAATGAGAT GCGTAGTCTT GGTGTGGACA TGGATGATAA AAACAATGCC 
CACTATGCAG TCCAGGCAAG AAGGTCTCGG AGTGTCACGA GGAAAAGAAA ACGAGAAGAA 
TCTGTTCCAC CCTCTTCCAC AGCGCGGAGT CGCAGTTGTT CTAGAACTCC ACGTGATGTT 
TCTGGTCTTC GGGATGTCAA GATGGTGAAG AAAGCCAAGA CTATGATGAA GAAAGCTCAG 
AAGAAGATGA ATCGCTTGGG GAAGAAAGGG GAAGCTGATA GGCATGTGTT TGATATGAAG 
CCCAAGCACT TGCTGTCTGG GAAGAGGAAA GCTGGTAAAA AGGACAGGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171081.1
CDS	537..2270
Translation	MAHYNFKKITVVPSAKDIHPFYADLMNILYDKDHYKLALGQINIAKNLVDNVAKDYVRLMKYGDSLYRCKQLKRAALGRMCTIIKRQRQSLEYLEQVRQHLSRLPTIDPNTRTLLLCGYPNVGKSSFINKVTRADVDVQPYAFTTKSLFVGHMDYKYLRWQVVDTPGILDHPLEDRNTIEMQAITALAHLRAAVLYVMDLSEQCGHGLKEQLELFQNIRPLFINKPLIVVANKCDVKRIAELSEEDQKIFLDLQAEGFPVIETSTLTEEGVIQVKTEACDRLLAHRVETKMKGNKVNEVLNRLHLAVPNKRDDKERPPFIPEGVIARRKRMEIEEPKKKRERDLELEMGDDYILDLQKYWDLMNSSEKYDKIPEIWEGHNVADYIDPAIMKKLEELEKEEELRTAAGEYDSDSESEDEEMMEIRQLAKQIREKKKLKILQSKEKNKQGPRMPRTAKKVQRADLENEMRSLGVDMDDKNNAHYAVQARRSRSVTRKRKREESVPPSSTARSRSCSRTPRDVSGLRDVKMVKKAKTMMKKAQKKMNRLGKKGEADRHVFDMKPKHLLSGKRKAGKKDRR

Target ORF information:

RefSeq Version	XM_017315592.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTP binding protein 4 (Gtpbp4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315592.1

ORF Insert Sequence:

ATGGCGCATT ACAACTTCAA GAAGATTACG GTGGTGCCGT CTGCCAAGGA TATCCATCCG 
TTTTATGCTG ACTTGATGAA TATTCTCTAT GACAAGGATC ACTACAAGTT GGCTCTAGGT 
CAAATAAATA TTGCCAAAAA CTTGGTGGAC AATGTTGCTA AAGATTATGT GCGTCTTATG 
AAATATGGTG ATTCTCTGTA CCGATGCAAG CAGCTGAAGC GCGCGGCCCT TGGACGAATG 
TGTACTATTA TCAAGAGACA GAGGCAGAGC TTGGAGTATC TGGAACAAGT ACGTCAACAT 
TTATCCCGCT TGCCAACCAT TGATCCAAAT ACAAGGACTC TGCTTTTGTG TGGGTATCCA 
AATGTTGGGA AATCCAGCTT CATCAACAAG GTGACAAGAG CAGATGTGGA CGTTCAACCC 
TATGCATTTA CCACCAAATC TCTGTTTGTT GGGCACATGG ATTATAAGTA TCTGCGCTGG 
CAGGTTGTAG ATACCCCAGG GATTTTGGAT CATCCTTTGG AGGATCGGAA CACCATTGAG 
ATGCAGGCCA TCACAGCCCT GGCCCACCTC CGAGCTGCAG TTCTGTATGT GATGGATCTG 
TCTGAGCAGT GTGGACATGG GTTAAAGGAG CAGTTAGAAC TCTTCCAGAA TATCAGACCT 
CTGTTTATCA ACAAGCCTCT TATTGTTGTA GCAAACAAAT GTGATGTGAA GAGAATAGCT 
GAACTTTCTG AAGAAGATCA GAAAATATTT CTAGACTTGC AGGCTGAAGG ATTCCCTGTC 
ATCGAGACCA GTACCCTGAC TGAGGAAGGT GTCATTCAAG TTAAAACAGA GGCGTGTGAC 
AGGCTTTTGG CTCACCGAGT AGAAACGAAA ATGAAAGGAA ATAAAGTGAA TGAGGTTCTG 
AACAGATTGC ATTTAGCTGT GCCCAACAAG AGAGATGATA AGGAGAGACC CCCTTTCATC 
CCAGAAGGAG TGATAGCTCG AAGGAAGAGA ATGGAGATTG AAGAGCCCAA GAAGAAGAGG 
GAGCGAGATC TTGAGCTGGA AATGGGAGAT GATTACATTT TGGATCTTCA GAAATACTGG 
GACTTAATGA ATTCATCTGA GAAATATGAT AAGATACCAG AAATTTGGGA AGGCCACAAT 
GTTGCTGATT ATATTGATCC TGCTATCATG AAGAAATTAG AAGAGTTGGA AAAGGAAGAA 
GAGCTAAGAA CAGCTGCTGG GGAGTATGAC AGTGACTCTG AGAGTGAAGA CGAGGAAATG 
ATGGAAATCC GACAGCTGGC AAAGCAAATT AGAGAAAAGA AGAAGTTGAA AATTCTACAG 
TCCAAAGAAA AGAATAAACA GGGACCCAGG ATGCCCCGAA CTGCCAAGAA GGTTCAACGG 
GCAGATTTAG AGAATGAGAT GCGTAGTCTT GGTGTGGACA TGGATGATAA AAACAATGCC 
CACTATGCAG TCCAGGCAAG AAGGTCTCGG AGTGTCACGA GGAAAAGAAA ACGAGAAGAA 
TCTGTTCCAC CCTCTTCCAC AGCGCGGAGT CGCAGTTGTT CTAGAACTCC ACGTGATGTT 
TCTGGTCTTC GGGATGTCAA GATGGTGAAG AAAGCCAAGA CTATGATGAA GAAAGCTCAG 
AAGAAGATGA ATCGCTTGGG GAAGAAAGGG GAAGCTGATA GGCATGTGTT TGATATGAAG 
CCCAAGCACT TGCTGTCTGG GAAGAGGAAA GCTGGTAAAA AGGACAGGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gtpbp4 ( XM_017315592.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171081.1 Mus musculus GTP binding protein 4 (Gtpbp4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu78514
Clone ID Related Accession (Same CDS sequence)	XM_017315592.1 , XM_017315592.2
Accession Version	XM_017315592.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nucleolar GTP-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCATT ACAACTTCAA GAAGATTACG GTGGTGCCGT CTGCCAAGGA TATCCATCCG 
TTTTATGCTG ACTTGATGAA TATTCTCTAT GACAAGGATC ACTACAAGTT GGCTCTAGGT 
CAAATAAATA TTGCCAAAAA CTTGGTGGAC AATGTTGCTA AAGATTATGT GCGTCTTATG 
AAATATGGTG ATTCTCTGTA CCGATGCAAG CAGCTGAAGC GCGCGGCCCT TGGACGAATG 
TGTACTATTA TCAAGAGACA GAGGCAGAGC TTGGAGTATC TGGAACAAGT ACGTCAACAT 
TTATCCCGCT TGCCAACCAT TGATCCAAAT ACAAGGACTC TGCTTTTGTG TGGGTATCCA 
AATGTTGGGA AATCCAGCTT CATCAACAAG GTGACAAGAG CAGATGTGGA CGTTCAACCC 
TATGCATTTA CCACCAAATC TCTGTTTGTT GGGCACATGG ATTATAAGTA TCTGCGCTGG 
CAGGTTGTAG ATACCCCAGG GATTTTGGAT CATCCTTTGG AGGATCGGAA CACCATTGAG 
ATGCAGGCCA TCACAGCCCT GGCCCACCTC CGAGCTGCAG TTCTGTATGT GATGGATCTG 
TCTGAGCAGT GTGGACATGG GTTAAAGGAG CAGTTAGAAC TCTTCCAGAA TATCAGACCT 
CTGTTTATCA ACAAGCCTCT TATTGTTGTA GCAAACAAAT GTGATGTGAA GAGAATAGCT 
GAACTTTCTG AAGAAGATCA GAAAATATTT CTAGACTTGC AGGCTGAAGG ATTCCCTGTC 
ATCGAGACCA GTACCCTGAC TGAGGAAGGT GTCATTCAAG TTAAAACAGA GGCGTGTGAC 
AGGCTTTTGG CTCACCGAGT AGAAACGAAA ATGAAAGGAA ATAAAGTGAA TGAGGTTCTG 
AACAGATTGC ATTTAGCTGT GCCCAACAAG AGAGATGATA AGGAGAGACC CCCTTTCATC 
CCAGAAGGAG TGATAGCTCG AAGGAAGAGA ATGGAGATTG AAGAGCCCAA GAAGAAGAGG 
GAGCGAGATC TTGAGCTGGA AATGGGAGAT GATTACATTT TGGATCTTCA GAAATACTGG 
GACTTAATGA ATTCATCTGA GAAATATGAT AAGATACCAG AAATTTGGGA AGGCCACAAT 
GTTGCTGATT ATATTGATCC TGCTATCATG AAGAAATTAG AAGAGTTGGA AAAGGAAGAA 
GAGCTAAGAA CAGCTGCTGG GGAGTATGAC AGTGACTCTG AGAGTGAAGA CGAGGAAATG 
ATGGAAATCC GACAGCTGGC AAAGCAAATT AGAGAAAAGA AGAAGTTGAA AATTCTACAG 
TCCAAAGAAA AGAATAAACA GGGACCCAGG ATGCCCCGAA CTGCCAAGAA GGTTCAACGG 
GCAGATTTAG AGAATGAGAT GCGTAGTCTT GGTGTGGACA TGGATGATAA AAACAATGCC 
CACTATGCAG TCCAGGCAAG AAGGTCTCGG AGTGTCACGA GGAAAAGAAA ACGAGAAGAA 
TCTGTTCCAC CCTCTTCCAC AGCGCGGAGT CGCAGTTGTT CTAGAACTCC ACGTGATGTT 
TCTGGTCTTC GGGATGTCAA GATGGTGAAG AAAGCCAAGA CTATGATGAA GAAAGCTCAG 
AAGAAGATGA ATCGCTTGGG GAAGAAAGGG GAAGCTGATA GGCATGTGTT TGATATGAAG 
CCCAAGCACT TGCTGTCTGG GAAGAGGAAA GCTGGTAAAA AGGACAGGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171081.1
CDS	121..1854
Translation	MAHYNFKKITVVPSAKDIHPFYADLMNILYDKDHYKLALGQINIAKNLVDNVAKDYVRLMKYGDSLYRCKQLKRAALGRMCTIIKRQRQSLEYLEQVRQHLSRLPTIDPNTRTLLLCGYPNVGKSSFINKVTRADVDVQPYAFTTKSLFVGHMDYKYLRWQVVDTPGILDHPLEDRNTIEMQAITALAHLRAAVLYVMDLSEQCGHGLKEQLELFQNIRPLFINKPLIVVANKCDVKRIAELSEEDQKIFLDLQAEGFPVIETSTLTEEGVIQVKTEACDRLLAHRVETKMKGNKVNEVLNRLHLAVPNKRDDKERPPFIPEGVIARRKRMEIEEPKKKRERDLELEMGDDYILDLQKYWDLMNSSEKYDKIPEIWEGHNVADYIDPAIMKKLEELEKEEELRTAAGEYDSDSESEDEEMMEIRQLAKQIREKKKLKILQSKEKNKQGPRMPRTAKKVQRADLENEMRSLGVDMDDKNNAHYAVQARRSRSVTRKRKREESVPPSSTARSRSCSRTPRDVSGLRDVKMVKKAKTMMKKAQKKMNRLGKKGEADRHVFDMKPKHLLSGKRKAGKKDRR

Target ORF information:

RefSeq Version	XM_017315592.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTP binding protein 4 (Gtpbp4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315592.2

ORF Insert Sequence:

ATGGCGCATT ACAACTTCAA GAAGATTACG GTGGTGCCGT CTGCCAAGGA TATCCATCCG 
TTTTATGCTG ACTTGATGAA TATTCTCTAT GACAAGGATC ACTACAAGTT GGCTCTAGGT 
CAAATAAATA TTGCCAAAAA CTTGGTGGAC AATGTTGCTA AAGATTATGT GCGTCTTATG 
AAATATGGTG ATTCTCTGTA CCGATGCAAG CAGCTGAAGC GCGCGGCCCT TGGACGAATG 
TGTACTATTA TCAAGAGACA GAGGCAGAGC TTGGAGTATC TGGAACAAGT ACGTCAACAT 
TTATCCCGCT TGCCAACCAT TGATCCAAAT ACAAGGACTC TGCTTTTGTG TGGGTATCCA 
AATGTTGGGA AATCCAGCTT CATCAACAAG GTGACAAGAG CAGATGTGGA CGTTCAACCC 
TATGCATTTA CCACCAAATC TCTGTTTGTT GGGCACATGG ATTATAAGTA TCTGCGCTGG 
CAGGTTGTAG ATACCCCAGG GATTTTGGAT CATCCTTTGG AGGATCGGAA CACCATTGAG 
ATGCAGGCCA TCACAGCCCT GGCCCACCTC CGAGCTGCAG TTCTGTATGT GATGGATCTG 
TCTGAGCAGT GTGGACATGG GTTAAAGGAG CAGTTAGAAC TCTTCCAGAA TATCAGACCT 
CTGTTTATCA ACAAGCCTCT TATTGTTGTA GCAAACAAAT GTGATGTGAA GAGAATAGCT 
GAACTTTCTG AAGAAGATCA GAAAATATTT CTAGACTTGC AGGCTGAAGG ATTCCCTGTC 
ATCGAGACCA GTACCCTGAC TGAGGAAGGT GTCATTCAAG TTAAAACAGA GGCGTGTGAC 
AGGCTTTTGG CTCACCGAGT AGAAACGAAA ATGAAAGGAA ATAAAGTGAA TGAGGTTCTG 
AACAGATTGC ATTTAGCTGT GCCCAACAAG AGAGATGATA AGGAGAGACC CCCTTTCATC 
CCAGAAGGAG TGATAGCTCG AAGGAAGAGA ATGGAGATTG AAGAGCCCAA GAAGAAGAGG 
GAGCGAGATC TTGAGCTGGA AATGGGAGAT GATTACATTT TGGATCTTCA GAAATACTGG 
GACTTAATGA ATTCATCTGA GAAATATGAT AAGATACCAG AAATTTGGGA AGGCCACAAT 
GTTGCTGATT ATATTGATCC TGCTATCATG AAGAAATTAG AAGAGTTGGA AAAGGAAGAA 
GAGCTAAGAA CAGCTGCTGG GGAGTATGAC AGTGACTCTG AGAGTGAAGA CGAGGAAATG 
ATGGAAATCC GACAGCTGGC AAAGCAAATT AGAGAAAAGA AGAAGTTGAA AATTCTACAG 
TCCAAAGAAA AGAATAAACA GGGACCCAGG ATGCCCCGAA CTGCCAAGAA GGTTCAACGG 
GCAGATTTAG AGAATGAGAT GCGTAGTCTT GGTGTGGACA TGGATGATAA AAACAATGCC 
CACTATGCAG TCCAGGCAAG AAGGTCTCGG AGTGTCACGA GGAAAAGAAA ACGAGAAGAA 
TCTGTTCCAC CCTCTTCCAC AGCGCGGAGT CGCAGTTGTT CTAGAACTCC ACGTGATGTT 
TCTGGTCTTC GGGATGTCAA GATGGTGAAG AAAGCCAAGA CTATGATGAA GAAAGCTCAG 
AAGAAGATGA ATCGCTTGGG GAAGAAAGGG GAAGCTGATA GGCATGTGTT TGATATGAAG 
CCCAAGCACT TGCTGTCTGG GAAGAGGAAA GCTGGTAAAA AGGACAGGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Two orthogonal cleavages separate subunit RNAs in mouse ribosome biogenesis.
Nucleic acids research42(17)11180-91(2014)
Wang M,Anikin L,Pestov DG

Restricting conformational flexibility of the switch II region creates a dominant-inhibitory phenotype in Obg GTPase Nog1.
Molecular and cellular biology27(21)7735-44(2007 Nov)
Lapik YR,Misra JM,Lau LF,Pestov DG

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

The heme environment of mouse neuroglobin. Evidence for the presence of two conformations of the heme pocket.
The Journal of biological chemistry276(39)36377-82(2001 Sep)
Couture M,Burmester T,Hankeln T,Rousseau DL

Identification of a novel nuclear guanosine triphosphate-binding protein differentially expressed in renal disease.
Journal of the American Society of Nephrology : JASN12(5)883-90(2001 May)
Laping NJ,Olson BA,Zhu Y

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Gtpbp4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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