Prpf31 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prpf31
Entrez Gene ID	68988
Full Name	pre-mRNA processing factor 31
Synonyms	1500019O16Rik,2810404O06Rik,AW554706,PRP31,RP11
General protein information	Preferred Names pre-mRNA processing factor 31 Names U4/U6 small nuclear ribonucleoprotein Prp31 PRP31 pre-mRNA processing factor 31 homolog U4/U6 snRNP 61 kDa protein protein 61K
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001159714.1	NP_001153186.1	U4/U6 small nuclear ribonucleoprotein Prp31 isoform 2
NM_027328.4	NP_081604.3	U4/U6 small nuclear ribonucleoprotein Prp31 isoform 1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	prp-31	NP_491527.1
Oryza sativa (rice)	Os04g0555400	NP_001053516.1
Oryza sativa (rice)	Os07g0141600	NP_001058865.1
Pan troglodytes (chimpanzee)	PRPF31	XP_001174769.1
Canis lupus familiaris (dog)	PRPF31	XP_850917.1
Danio rerio (zebrafish)	prpf31	NP_956798.1
Xenopus tropicalis (tropical clawed frog)	prpf31	NP_998859.1
Homo sapiens (human)	PRPF31	NP_056444.3
Bos taurus (cattle)	PRPF31	NP_001193214.1
Rattus norvegicus (Norway rat)	Prpf31	NP_001099689.1
Schizosaccharomyces pombe (fission yeast)	prp31	NP_595294.1
Magnaporthe oryzae (rice blast fungus)	MGG_13154	XP_003710621.1
Arabidopsis thaliana (thale cress)	emb1220	NP_564754.1
	PRPF31	XP_001116111.2
Mus musculus (house mouse)	Prpf31	NP_081604.3
Drosophila melanogaster (fruit fly)	Prp31	NP_648756.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012142	XP_320385.4
Neurospora crassa	NCU02716	XP_965101.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Mutations in pre-mRNA processing factors 3, 8, and 31 cause dysfunction of the retinal pigment epithelium.
Farkas MH, Lew DS, Sousa ME, Bujakowska K, Chatagnon J, Bhattacharya SS, Pierce EA, Nandrot EF
The American journal of pathology184(10)2641-52(2014 Oct)

CTNNBL1 is a novel nuclear localization sequence-binding protein that recognizes RNA-splicing factors CDC5L and Prp31.
Ganesh K, Adam S, Taylor B, Simpson P, Rada C, Neuberger M
The Journal of biological chemistry286(19)17091-102(2011 May)

Temporal and tissue specific regulation of RP-associated splicing factor genes PRPF3, PRPF31 and PRPC8--implications in the pathogenesis of RP.
Cao H, Wu J, Lam S, Duan R, Newnham C, Molday RS, Graziotto JJ, Pierce EA, Hu J
PloS one6(1)e15860(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The mouse retinal pigment epithelium (RPE)is the primary cell affected by mutations in the RNA splicing factors (PRPF3, PRPF8, and PRPF31), and these changes occur at an early age.
Title: Mutations in pre-mRNA processing factors 3, 8, and 31 cause dysfunction of the retinal pigment epithelium.

CTNNBL1 is a novel nuclear localization sequence-binding protein that recognizes RNA-splicing factors CDC5L and Prp31
Title: CTNNBL1 is a novel nuclear localization sequence-binding protein that recognizes RNA-splicing factors CDC5L and Prp31.

The finding of similar degenerative changes in RPE cells of all three mouse models suggests that the retinal pigment epithelium may be the primary cell type affected in the RNA splicing factor forms of retinitis pigmentosa.
Title: Three gene-targeted mouse models of RNA splicing factor RP show late-onset RPE and retinal degeneration.

found that PRP31 and PRPC8 as well as snRNAs are highly expressed in retinal cells
Title: Temporal and tissue specific regulation of RP-associated splicing factor genes PRPF3, PRPF31 and PRPC8--implications in the pathogenesis of RP.

The results imply that Prpf31 is necessary for survival, and there is no compensation mechanism in mouse for the lack of this splicing factor
Title: Study of gene-targeted mouse models of splicing factor gene Prpf31 implicated in human autosomal dominant retinitis pigmentosa (RP).

The following Prpf31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prpf31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15828	NM_001159714.1 Latest version!	Mus musculus pre-mRNA processing factor 31 (Prpf31), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu16292	NM_027328.4 Latest version!	Mus musculus pre-mRNA processing factor 31 (Prpf31), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prpf31 ( NM_001159714.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153186.1 Mus musculus pre-mRNA processing factor 31 (Prpf31), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15828
Clone ID Related Accession (Same CDS sequence)	NM_001159714.1
Accession Version	NM_001159714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	U4/U6 small nuclear ribonucleoprotein Prp31 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB839183.1, BC061461.1 and AC171680.5. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region compared to variant 1. This results in a shorter protein (isoform b) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC061461.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTGG CTGATGAGCT CTTGGCTGAC CTCGAGGAGG CAGCAGAAGA AGAGGAAGGA 
GGAAGCTATG GAGAAGAGGA GGAGGAGCCA GCAATTGAGG ATGTACAGGA GGAGACCCAG 
CTGGATCTTT CTGGAGATTC AGTCAAGAGC ATTGCCAAGC TATGGGACAG CAAGATGTTT 
GCTGAGATCA TGATGAAGAT TGAGGAGTAT ATCAGCAAGC AAGCCAACGT GTCGGAAGTG 
ATGGGACCAG TGGAGGCAGC TCCTGAGTAC CGAGTCATTG TGGATGCCAA CAACCTGACT 
GTGGAGATAG AAAATGAGCT GAACATTATC CACAAGTTCA TCCGGGATAA ATACTCAAAG 
AGATTCCCTG AGCTAGAATC CTTGGTGCCC AATGCTCTGG ATTACATCCG CACAGTCAAG 
GAGCTGGGCA ACAGCCTGGA TAAGTGCAAG AACAATGAGA ACCTCCAGCA GATCCTGACC 
AATGCTACTA TCATGGTTGT TAGTGTCACT GCCTCTACTA CCCAGGGGCA GCAGCTGTCG 
GATGAGGAGC TAGAGCGGCT AGAGGAGGCC TGTGACATGG CACTGGAGCT GAATGCCTCC 
AAACACCGCA TCTATGAGTA TGTGGAGTCC CGGATGTCCT TCATTGCACC CAACTTGTCC 
ATCATCATTG GAGCATCCAC AGCTGCCAAG ATCATGGGTG TGGCTGGAGG GCTCACCAAC 
CTCTCTAAGA TGCCTGCCTG CAACATCATG CTGCTGGGAG CCCAGCGCAA GACCCTGTCT 
GGCTTCTCTT CTACCTCAGT GTTGCCTCAT ACTGGTTATA TCTACCACAG TGACATTGTG 
CAGTCTCTGC CCCCAGATCT CCGGCGGAAG GCGGCCCGCC TAGTGGCTGC CAAGTGTACA 
CTGGCAGCTC GTGTGGACAG CTTCCATGAG AGCACAGAAG GGAAGGATGA GATTGAGCGC 
AAATTTGATA AGTGGCAGGA GCCACCACCC GTGAAGCAGG TGAAGCCCCT GCCTGCACCC 
CTTGATGGGC AGCGGAAGAA GCGAGGGGGC CGAAGGTACC GCAAGATGAA GGAACGGCTA 
GGACTGACGG AGATCCGGAA GCAGGCCAAC CGCATGAGCT TTGGAGAGAT TGAGGAGGAT 
GCCTATCAGG AAGACCTGGG CTTTAGCCTG GGCCACTTGG GCAAGTCAGG CAGTGGGCGT 
GTGCGGCAGA CCCAGGTGAA TGAGGCCACC AAGGCCAGGA TCTCCAAGAC ACTGCAGCGG 
ACCTTACAGA AACAGAGCGT GGTGTATGGT GGGAAGTCAA CAATTCGTGA CCGCTCCTCA 
GGGACTGCCT CCAGTGTGGC CTTCACTCCA CTCCAGGGTC TAGAGATTGT GAACCCACAA 
GCAGCCGAGA AGAAGGTGGC AGAGGCCAAC CAGAAGTATT TCTCTAGCAT GGCCGAGTTC 
CTCAAGGTCA AGGGTGAGAA AAGTGGCACC ATGTCTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153186.1
CDS	81..1562
Translation	MSLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSGDSVKSIAKLWDSKMFAEIMMKIEEYISKQANVSEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSDEELERLEEACDMALELNASKHRIYEYVESRMSFIAPNLSIIIGASTAAKIMGVAGGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLRRKAARLVAAKCTLAARVDSFHESTEGKDEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGGRRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRVRQTQVNEATKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEANQKYFSSMAEFLKVKGEKSGTMST

Target ORF information:

RefSeq Version	NM_001159714.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pre-mRNA processing factor 31 (Prpf31), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159714.1

ORF Insert Sequence:

ATGTCTCTGG CTGATGAGCT CTTGGCTGAC CTCGAGGAGG CAGCAGAAGA AGAGGAAGGA 
GGAAGCTATG GAGAAGAGGA GGAGGAGCCA GCAATTGAGG ATGTACAGGA GGAGACCCAG 
CTGGATCTTT CTGGAGATTC AGTCAAGAGC ATTGCCAAGC TATGGGACAG CAAGATGTTT 
GCTGAGATCA TGATGAAGAT TGAGGAGTAT ATCAGCAAGC AAGCCAACGT GTCGGAAGTG 
ATGGGACCAG TGGAGGCAGC TCCTGAGTAC CGAGTCATTG TGGATGCCAA CAACCTGACT 
GTGGAGATAG AAAATGAGCT GAACATTATC CACAAGTTCA TCCGGGATAA ATACTCAAAG 
AGATTCCCTG AGCTAGAATC CTTGGTGCCC AATGCTCTGG ATTACATCCG CACAGTCAAG 
GAGCTGGGCA ACAGCCTGGA TAAGTGCAAG AACAATGAGA ACCTCCAGCA GATCCTGACC 
AATGCTACTA TCATGGTTGT TAGTGTCACT GCCTCTACTA CCCAGGGGCA GCAGCTGTCG 
GATGAGGAGC TAGAGCGGCT AGAGGAGGCC TGTGACATGG CACTGGAGCT GAATGCCTCC 
AAACACCGCA TCTATGAGTA TGTGGAGTCC CGGATGTCCT TCATTGCACC CAACTTGTCC 
ATCATCATTG GAGCATCCAC AGCTGCCAAG ATCATGGGTG TGGCTGGAGG GCTCACCAAC 
CTCTCTAAGA TGCCTGCCTG CAACATCATG CTGCTGGGAG CCCAGCGCAA GACCCTGTCT 
GGCTTCTCTT CTACCTCAGT GTTGCCTCAT ACTGGTTATA TCTACCACAG TGACATTGTG 
CAGTCTCTGC CCCCAGATCT CCGGCGGAAG GCGGCCCGCC TAGTGGCTGC CAAGTGTACA 
CTGGCAGCTC GTGTGGACAG CTTCCATGAG AGCACAGAAG GGAAGGATGA GATTGAGCGC 
AAATTTGATA AGTGGCAGGA GCCACCACCC GTGAAGCAGG TGAAGCCCCT GCCTGCACCC 
CTTGATGGGC AGCGGAAGAA GCGAGGGGGC CGAAGGTACC GCAAGATGAA GGAACGGCTA 
GGACTGACGG AGATCCGGAA GCAGGCCAAC CGCATGAGCT TTGGAGAGAT TGAGGAGGAT 
GCCTATCAGG AAGACCTGGG CTTTAGCCTG GGCCACTTGG GCAAGTCAGG CAGTGGGCGT 
GTGCGGCAGA CCCAGGTGAA TGAGGCCACC AAGGCCAGGA TCTCCAAGAC ACTGCAGCGG 
ACCTTACAGA AACAGAGCGT GGTGTATGGT GGGAAGTCAA CAATTCGTGA CCGCTCCTCA 
GGGACTGCCT CCAGTGTGGC CTTCACTCCA CTCCAGGGTC TAGAGATTGT GAACCCACAA 
GCAGCCGAGA AGAAGGTGGC AGAGGCCAAC CAGAAGTATT TCTCTAGCAT GGCCGAGTTC 
CTCAAGGTCA AGGGTGAGAA AAGTGGCACC ATGTCTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prpf31 ( NM_027328.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081604.3 Mus musculus pre-mRNA processing factor 31 (Prpf31), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16292
Clone ID Related Accession (Same CDS sequence)	NM_027328.4
Accession Version	NM_027328.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	U4/U6 small nuclear ribonucleoprotein Prp31 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB839183.1, AK033283.1, AK051260.1 and AC171680.5. On Apr 30, 2009 this sequence version replaced NM_027328.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK051260.1, AK033190.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTGG CTGATGAGCT CTTGGCTGAC CTCGAGGAGG CAGCAGAAGA AGAGGAAGGA 
GGAAGCTATG GAGAAGAGGA GGAGGAGCCA GCAATTGAGG ATGTACAGGA GGAGACCCAG 
CTGGATCTTT CTGGAGATTC AGTCAAGAGC ATTGCCAAGC TATGGGACAG CAAGATGTTT 
GCTGAGATCA TGATGAAGAT TGAGGAGTAT ATCAGCAAGC AAGCCAACGT GTCGGAAGTG 
ATGGGACCAG TGGAGGCAGC TCCTGAGTAC CGAGTCATTG TGGATGCCAA CAACCTGACT 
GTGGAGATAG AAAATGAGCT GAACATTATC CACAAGTTCA TCCGGGATAA ATACTCAAAG 
AGATTCCCTG AGCTAGAATC CTTGGTGCCC AATGCTCTGG ATTACATCCG CACAGTCAAG 
GAGCTGGGCA ACAGCCTGGA TAAGTGCAAG AACAATGAGA ACCTCCAGCA GATCCTGACC 
AATGCTACTA TCATGGTTGT TAGTGTCACT GCCTCTACTA CCCAGGGGCA GCAGCTGTCG 
GATGAGGAGC TAGAGCGGCT AGAGGAGGCC TGTGACATGG CACTGGAGCT GAATGCCTCC 
AAACACCGCA TCTATGAGTA TGTGGAGTCC CGGATGTCCT TCATTGCACC CAACTTGTCC 
ATCATCATTG GAGCATCCAC AGCTGCCAAG ATCATGGGTG TGGCTGGAGG GCTCACCAAC 
CTCTCTAAGA TGCCTGCCTG CAACATCATG CTGCTGGGAG CCCAGCGCAA GACCCTGTCT 
GGCTTCTCTT CTACCTCAGT GTTGCCTCAT ACTGGTTATA TCTACCACAG TGACATTGTG 
CAGTCTCTGC CCCCAGATCT CCGGCGGAAG GCGGCCCGCC TAGTGGCTGC CAAGTGTACA 
CTGGCAGCTC GTGTGGACAG CTTCCATGAG AGCACAGAAG GGAAGGTGGG CTACGAACTA 
AAGGATGAGA TTGAGCGCAA ATTTGATAAG TGGCAGGAGC CACCACCCGT GAAGCAGGTG 
AAGCCCCTGC CTGCACCCCT TGATGGGCAG CGGAAGAAGC GAGGGGGCCG AAGGTACCGC 
AAGATGAAGG AACGGCTAGG ACTGACGGAG ATCCGGAAGC AGGCCAACCG CATGAGCTTT 
GGAGAGATTG AGGAGGATGC CTATCAGGAA GACCTGGGCT TTAGCCTGGG CCACTTGGGC 
AAGTCAGGCA GTGGGCGTGT GCGGCAGACC CAGGTGAATG AGGCCACCAA GGCCAGGATC 
TCCAAGACAC TGCAGCGGAC CTTACAGAAA CAGAGCGTGG TGTATGGTGG GAAGTCAACA 
ATTCGTGACC GCTCCTCAGG GACTGCCTCC AGTGTGGCCT TCACTCCACT CCAGGGTCTA 
GAGATTGTGA ACCCACAAGC AGCCGAGAAG AAGGTGGCAG AGGCCAACCA GAAGTATTTC 
TCTAGCATGG CCGAGTTCCT CAAGGTCAAG GGTGAGAAAA GTGGCACCAT GTCTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081604.3
CDS	81..1580
Translation	MSLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSGDSVKSIAKLWDSKMFAEIMMKIEEYISKQANVSEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSDEELERLEEACDMALELNASKHRIYEYVESRMSFIAPNLSIIIGASTAAKIMGVAGGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLRRKAARLVAAKCTLAARVDSFHESTEGKVGYELKDEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGGRRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRVRQTQVNEATKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEANQKYFSSMAEFLKVKGEKSGTMST

Target ORF information:

RefSeq Version	NM_027328.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus pre-mRNA processing factor 31 (Prpf31), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027328.4

ORF Insert Sequence:

ATGTCTCTGG CTGATGAGCT CTTGGCTGAC CTCGAGGAGG CAGCAGAAGA AGAGGAAGGA 
GGAAGCTATG GAGAAGAGGA GGAGGAGCCA GCAATTGAGG ATGTACAGGA GGAGACCCAG 
CTGGATCTTT CTGGAGATTC AGTCAAGAGC ATTGCCAAGC TATGGGACAG CAAGATGTTT 
GCTGAGATCA TGATGAAGAT TGAGGAGTAT ATCAGCAAGC AAGCCAACGT GTCGGAAGTG 
ATGGGACCAG TGGAGGCAGC TCCTGAGTAC CGAGTCATTG TGGATGCCAA CAACCTGACT 
GTGGAGATAG AAAATGAGCT GAACATTATC CACAAGTTCA TCCGGGATAA ATACTCAAAG 
AGATTCCCTG AGCTAGAATC CTTGGTGCCC AATGCTCTGG ATTACATCCG CACAGTCAAG 
GAGCTGGGCA ACAGCCTGGA TAAGTGCAAG AACAATGAGA ACCTCCAGCA GATCCTGACC 
AATGCTACTA TCATGGTTGT TAGTGTCACT GCCTCTACTA CCCAGGGGCA GCAGCTGTCG 
GATGAGGAGC TAGAGCGGCT AGAGGAGGCC TGTGACATGG CACTGGAGCT GAATGCCTCC 
AAACACCGCA TCTATGAGTA TGTGGAGTCC CGGATGTCCT TCATTGCACC CAACTTGTCC 
ATCATCATTG GAGCATCCAC AGCTGCCAAG ATCATGGGTG TGGCTGGAGG GCTCACCAAC 
CTCTCTAAGA TGCCTGCCTG CAACATCATG CTGCTGGGAG CCCAGCGCAA GACCCTGTCT 
GGCTTCTCTT CTACCTCAGT GTTGCCTCAT ACTGGTTATA TCTACCACAG TGACATTGTG 
CAGTCTCTGC CCCCAGATCT CCGGCGGAAG GCGGCCCGCC TAGTGGCTGC CAAGTGTACA 
CTGGCAGCTC GTGTGGACAG CTTCCATGAG AGCACAGAAG GGAAGGTGGG CTACGAACTA 
AAGGATGAGA TTGAGCGCAA ATTTGATAAG TGGCAGGAGC CACCACCCGT GAAGCAGGTG 
AAGCCCCTGC CTGCACCCCT TGATGGGCAG CGGAAGAAGC GAGGGGGCCG AAGGTACCGC 
AAGATGAAGG AACGGCTAGG ACTGACGGAG ATCCGGAAGC AGGCCAACCG CATGAGCTTT 
GGAGAGATTG AGGAGGATGC CTATCAGGAA GACCTGGGCT TTAGCCTGGG CCACTTGGGC 
AAGTCAGGCA GTGGGCGTGT GCGGCAGACC CAGGTGAATG AGGCCACCAA GGCCAGGATC 
TCCAAGACAC TGCAGCGGAC CTTACAGAAA CAGAGCGTGG TGTATGGTGG GAAGTCAACA 
ATTCGTGACC GCTCCTCAGG GACTGCCTCC AGTGTGGCCT TCACTCCACT CCAGGGTCTA 
GAGATTGTGA ACCCACAAGC AGCCGAGAAG AAGGTGGCAG AGGCCAACCA GAAGTATTTC 
TCTAGCATGG CCGAGTTCCT CAAGGTCAAG GGTGAGAAAA GTGGCACCAT GTCTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Mutations in pre-mRNA processing factors 3, 8, and 31 cause dysfunction of the retinal pigment epithelium.
The American journal of pathology184(10)2641-52(2014 Oct)
Farkas MH,Lew DS,Sousa ME,Bujakowska K,Chatagnon J,Bhattacharya SS,Pierce EA,Nandrot EF

CTNNBL1 is a novel nuclear localization sequence-binding protein that recognizes RNA-splicing factors CDC5L and Prp31.
The Journal of biological chemistry286(19)17091-102(2011 May)
Ganesh K,Adam S,Taylor B,Simpson P,Rada C,Neuberger M

Temporal and tissue specific regulation of RP-associated splicing factor genes PRPF3, PRPF31 and PRPC8--implications in the pathogenesis of RP.
PloS one6(1)e15860(2011 Jan)
Cao H,Wu J,Lam S,Duan R,Newnham C,Molday RS,Graziotto JJ,Pierce EA,Hu J

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Prpf31 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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