prpf31 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	prpf31
Entrez Gene ID	393476
Full Name	PRP31 pre-mRNA processing factor 31 homolog (yeast)
Synonyms	zgc:66177
General protein information	Preferred Names PRP31 pre-mRNA processing factor 31 homolog (yeast) Names U4/U6 small nuclear ribonucleoprotein Prp31 pre-mRNA-processing factor 31
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200504.1	NP_956798.1	U4/U6 small nuclear ribonucleoprotein Prp31
NM_200504.2	NP_956798.1	U4/U6 small nuclear ribonucleoprotein Prp31

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	prp-31	NP_491527.1
Oryza sativa (rice)	Os04g0555400	NP_001053516.1
Oryza sativa (rice)	Os07g0141600	NP_001058865.1
Pan troglodytes (chimpanzee)	PRPF31	XP_001174769.1
Canis lupus familiaris (dog)	PRPF31	XP_850917.1
Danio rerio (zebrafish)	prpf31	NP_956798.1
Xenopus tropicalis (tropical clawed frog)	prpf31	NP_998859.1
Homo sapiens (human)	PRPF31	NP_056444.3
Bos taurus (cattle)	PRPF31	NP_001193214.1
Rattus norvegicus (Norway rat)	Prpf31	NP_001099689.1
Schizosaccharomyces pombe (fission yeast)	prp31	NP_595294.1
Magnaporthe oryzae (rice blast fungus)	MGG_13154	XP_003710621.1
Arabidopsis thaliana (thale cress)	emb1220	NP_564754.1
	PRPF31	XP_001116111.2
Mus musculus (house mouse)	Prpf31	NP_081604.3
Drosophila melanogaster (fruit fly)	Prp31	NP_648756.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012142	XP_320385.4
Neurospora crassa	NCU02716	XP_965101.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Knocking Down Snrnp200 Initiates Demorphogenesis of Rod Photoreceptors in Zebrafish.
Liu Y, Chen X, Qin B, Zhao K, Zhao Q, Staley JP, Zhao C
Journal of ophthalmology2015816329(2015)

PRPF4 mutations cause autosomal dominant retinitis pigmentosa.
Chen X, Liu Y, Sheng X, Tam PO, Zhao K, Chen X, Rong W, Liu Y, Liu X, Pan X, Chen LJ, Zhao Q, Vollrath D, Pang CP, Zhao C
Human molecular genetics23(11)2926-39(2014 Jun)

Mutant Prpf31 causes pre-mRNA splicing defects and rod photoreceptor cell degeneration in a zebrafish model for Retinitis pigmentosa.
Yin J, Brocher J, Fischer U, Winkler C
Molecular neurodegeneration656(2011 Jul)

Systemic splicing factor deficiency causes tissue-specific defects: a zebrafish model for retinitis pigmentosa.
Linder B, Dill H, Hirmer A, Brocher J, Lee GP, Mathavan S, Bolz HJ, Winkler C, Laggerbauer B, Fischer U
Human molecular genetics20(2)368-77(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following prpf31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prpf31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01911	NM_200504.1 Latest version!	Danio rerio PRP31 pre-mRNA processing factor 31 homolog (yeast) (prpf31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa159857	NM_200504.2 Latest version!	Danio rerio PRP31 pre-mRNA processing factor 31 homolog (yeast) (prpf31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prpf31 ( NM_200504.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956798.1 Danio rerio PRP31 pre-mRNA processing factor 31 homolog (yeast) (prpf31), mRNA.

Price & Availability↑

CloneID	ODa01911
Clone ID Related Accession (Same CDS sequence)	NM_200504.1
Accession Version	NM_200504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Danio rerio(zebrafish)
Product	U4/U6 small nuclear ribonucleoprotein Prp31
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC055531.1. ##Evidence-Data-START## Transcript exon combination :: BC055531.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCTTTGG CAGACGAGCT GCTTGCGGAT TTGGAGGAGG CGGGAGAAGA GGACGGGCTG 
TATCCCGGCG GAGAAGAGGG AGAGAGTGAT GGAGAGCCCG GAGAAAGACA AGTTGATGGA 
GGACTGGAGG ACATCCCGGA GGAGATGGAG GTGGATTACA GCAGCACGGA GAGCGTCACG 
TCCATAGCTA AACTACGACA CAGCAAACCG TTCGCTGAAA TCATGGATAA AATCAGTCAC 
TATGTTGGAA ATCAACGCAA GAATTCAGAA GTGTCTGGAC CGGTTGAAGC AGACCCAGAG 
TACAGGCTCA TTGTTGCCGC CAACAATCTG ACGGTGGAAA TAGACAACGA GCTTAATATC 
ATTCACAAGT TTGTTCGTGA CAAGTACTCC AAAAGGTTTC CTGAGCTGGA GTCTCTGGTG 
CCAAATGCAC TGGACTACAT CAGAACTGTT AAGGAGCTGG GCAACAATCT GGAAAAGTGC 
AAAAACAATG AGACCCTTCA GCAGATTCTC ACCAATGCCA CCATTATGGT GGTCAGCGTC 
ACAGCCTCTA CCACACAAGG AACGATGCTT GGAGATGATG AGCTCCAGCG ATTGGAGGAG 
GCCTGTGACA TGGCCCTTGA GCTCAACCAA TCAAAACACA GGATCTATGA ATATGTGGAA 
TCCAGAATGT CATTCATTGC TCCGAATCTT TCAATTATTG TCGGAGCTTC AACTGCTGCC 
AAGATCATGG GTGTTGCTGG TGGTCTGACT AACCTGTCCA AGATGCCCGC ATGTAATCTG 
ATGTTGCTGG GAGCGCAGAG GAGAACACTG TCTGGATTCA GCAGCACATC TCTGCTGCCC 
CACACAGGCT ACATTTACCA CTGTGATGTA GTGCAGACTC TGCCTCCAGA TCTAAGGAGG 
AAAGCAGCTC GTCTGGTTTC TGCTAAATGC ACTCTGGCTT CTCGCGTGGA CAGTTTTCAT 
GAGAGCGCAG ATGGCAAGGT TGGCTATGAT TTGAAGGAAG AGATTGAGAG GAAGTTTGAT 
AAATGGCAGG AGCCTCCACC AGTCAAGCAG GTCAAGCCTC TTCCAGCTCC TCTGGATGGA 
CAGAGGAAGA AGAGAGGAGG AAGAAGGTAT CGTAAGATGA AAGAAAGACT GGGACTGACA 
GAGATCAGGA AACACGCCAA CAGAATGACG TTTGCTGAGA TTGAGGACGA TGCCTATCAG 
GAGGATCTGG GCTTCAGTCT CGGTCAGCTG GGAAAATCTG GCAGTGGCAG AGTCAGACAA 
GCTCAGGTCA ACGACTCCAC CAAAGCCAGA ATATCCAAAT CTTTACAGAG GACACTGCAG 
AAGCAGAGCA TGACGTACGG AGGCAAGTCT ACAGTGAGAG ACCGATCGTC TGGAACCAGC 
TCCAGCGTGG CCTTCACTCC TCTACAGGGG CTGGAGATTG TGAACCCACA GGCTGCAGAG 
AAGAAGGTGG CTGAAGCCAA TCAGAAATAT TTCTCCAACA TGGCTGAGTT CCTCAAGGTC 
AAGCGTGAGA AGGAGGACAA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956798.1
CDS	75..1601
Translation	MSLADELLADLEEAGEEDGLYPGGEEGESDGEPGERQVDGGLEDIPEEMEVDYSSTESVTSIAKLRHSKPFAEIMDKISHYVGNQRKNSEVSGPVEADPEYRLIVAANNLTVEIDNELNIIHKFVRDKYSKRFPELESLVPNALDYIRTVKELGNNLEKCKNNETLQQILTNATIMVVSVTASTTQGTMLGDDELQRLEEACDMALELNQSKHRIYEYVESRMSFIAPNLSIIVGASTAAKIMGVAGGLTNLSKMPACNLMLLGAQRRTLSGFSSTSLLPHTGYIYHCDVVQTLPPDLRRKAARLVSAKCTLASRVDSFHESADGKVGYDLKEEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGGRRYRKMKERLGLTEIRKHANRMTFAEIEDDAYQEDLGFSLGQLGKSGSGRVRQAQVNDSTKARISKSLQRTLQKQSMTYGGKSTVRDRSSGTSSSVAFTPLQGLEIVNPQAAEKKVAEANQKYFSNMAEFLKVKREKEDKV

Target ORF information:

RefSeq Version	NM_200504.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio PRP31 pre-mRNA processing factor 31 homolog (yeast) (prpf31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200504.1

ORF Insert Sequence:

ATGTCTTTGG CAGACGAGCT GCTTGCGGAT TTGGAGGAGG CGGGAGAAGA GGACGGGCTG 
TATCCCGGCG GAGAAGAGGG AGAGAGTGAT GGAGAGCCCG GAGAAAGACA AGTTGATGGA 
GGACTGGAGG ACATCCCGGA GGAGATGGAG GTGGATTACA GCAGCACGGA GAGCGTCACG 
TCCATAGCTA AACTACGACA CAGCAAACCG TTCGCTGAAA TCATGGATAA AATCAGTCAC 
TATGTTGGAA ATCAACGCAA GAATTCAGAA GTGTCTGGAC CGGTTGAAGC AGACCCAGAG 
TACAGGCTCA TTGTTGCCGC CAACAATCTG ACGGTGGAAA TAGACAACGA GCTTAATATC 
ATTCACAAGT TTGTTCGTGA CAAGTACTCC AAAAGGTTTC CTGAGCTGGA GTCTCTGGTG 
CCAAATGCAC TGGACTACAT CAGAACTGTT AAGGAGCTGG GCAACAATCT GGAAAAGTGC 
AAAAACAATG AGACCCTTCA GCAGATTCTC ACCAATGCCA CCATTATGGT GGTCAGCGTC 
ACAGCCTCTA CCACACAAGG AACGATGCTT GGAGATGATG AGCTCCAGCG ATTGGAGGAG 
GCCTGTGACA TGGCCCTTGA GCTCAACCAA TCAAAACACA GGATCTATGA ATATGTGGAA 
TCCAGAATGT CATTCATTGC TCCGAATCTT TCAATTATTG TCGGAGCTTC AACTGCTGCC 
AAGATCATGG GTGTTGCTGG TGGTCTGACT AACCTGTCCA AGATGCCCGC ATGTAATCTG 
ATGTTGCTGG GAGCGCAGAG GAGAACACTG TCTGGATTCA GCAGCACATC TCTGCTGCCC 
CACACAGGCT ACATTTACCA CTGTGATGTA GTGCAGACTC TGCCTCCAGA TCTAAGGAGG 
AAAGCAGCTC GTCTGGTTTC TGCTAAATGC ACTCTGGCTT CTCGCGTGGA CAGTTTTCAT 
GAGAGCGCAG ATGGCAAGGT TGGCTATGAT TTGAAGGAAG AGATTGAGAG GAAGTTTGAT 
AAATGGCAGG AGCCTCCACC AGTCAAGCAG GTCAAGCCTC TTCCAGCTCC TCTGGATGGA 
CAGAGGAAGA AGAGAGGAGG AAGAAGGTAT CGTAAGATGA AAGAAAGACT GGGACTGACA 
GAGATCAGGA AACACGCCAA CAGAATGACG TTTGCTGAGA TTGAGGACGA TGCCTATCAG 
GAGGATCTGG GCTTCAGTCT CGGTCAGCTG GGAAAATCTG GCAGTGGCAG AGTCAGACAA 
GCTCAGGTCA ACGACTCCAC CAAAGCCAGA ATATCCAAAT CTTTACAGAG GACACTGCAG 
AAGCAGAGCA TGACGTACGG AGGCAAGTCT ACAGTGAGAG ACCGATCGTC TGGAACCAGC 
TCCAGCGTGG CCTTCACTCC TCTACAGGGG CTGGAGATTG TGAACCCACA GGCTGCAGAG 
AAGAAGGTGG CTGAAGCCAA TCAGAAATAT TTCTCCAACA TGGCTGAGTT CCTCAAGGTC 
AAGCGTGAGA AGGAGGACAA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prpf31 ( NM_200504.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956798.1 Danio rerio PRP31 pre-mRNA processing factor 31 homolog (yeast) (prpf31), mRNA.

Price & Availability↑

CloneID	ODa159857
Clone ID Related Accession (Same CDS sequence)	NM_200504.2
Accession Version	NM_200504.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Danio rerio(zebrafish)
Product	U4/U6 small nuclear ribonucleoprotein Prp31
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR854927.24. On Apr 13, 2018 this sequence version replaced NM_200504.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC052224.1, GDQQ01000956.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTTGG CAGACGAGCT GCTTGCGGAT TTGGAGGAGG CAGGAGAAGA GGACGGGCTG 
TATCCCGGCG GAGAAGAGGG AGAGAGTGAT GGAGAGCCCG GAGAGAGACA GGTTGATGGA 
GGACTGGAGG ACATCCCGGA GGAGATGGAG GTGGATTACA GCAGCACGGA GAGCGTCACG 
TCCATAGCTA AACTACGACA CAGCAAACCG TTCGCTGAAA TCATGGATAA AATCAGTCAC 
TATGTTGGAA ATCAACGCAA GAATTCAGAA GTGTCTGGAC CGGTTGAAGC AGACCCAGAG 
TACAGGCTCA TTGTTGCCGC CAACAATCTG ACGGTGGAAA TAGACAACGA GCTTAATATC 
ATTCACAAGT TTGTTCGTGA CAAGTACTCC AAAAGGTTTC CTGAGCTGGA GTCTCTGGTG 
CCAAATGCAC TGGACTACAT CAGAACTGTT AAGGAGCTGG GCAACAATCT GGAAAAGTGC 
AAAAACAATG AGACCCTTCA GCAGATTCTC ACCAATGCCA CCATTATGGT GGTCAGCGTC 
ACAGCCTCTA CCACACAAGG AACGATGCTT GGAGATGATG AGCTCCAGCG ATTGGAGGAG 
GCCTGTGACA TGGCCCTTGA GCTCAACCAA TCAAAACACA GGATCTATGA ATATGTGGAA 
TCCAGAATGT CATTCATTGC TCCGAATCTT TCAATTATTG TCGGAGCTTC AACTGCTGCA 
AAGATCATGG GTGTTGCTGG TGGTCTGACT AACCTGTCCA AGATGCCCGC ATGTAATCTG 
ATGTTGCTGG GAGCGCAGAG GAGAACACTG TCTGGATTCA GCAGCACATC TCTGCTGCCC 
CACACAGGCT ACATCTACCA CTGTGATGTA GTGCAGACTC TGCCTCCAGA TCTAAGGAGG 
AAAGCAGCTC GTCTGGTTTC TGCTAAATGC ACTCTGGCTT CTCGCGTGGA CAGTTTTCAT 
GAGAGCGCAG ATGGCAAGGT TGGCTATGAT TTGAAGGAAG AGATTGAGAG GAAGTTTGAT 
AAATGGCAGG AGCCTCCACC AGTCAAGCAG GTCAAGCCTC TTCCAGCTCC TCTGGATGGA 
CAGAGGAAGA AGAGAGGAGG AAGAAGGTAT CGTAAGATGA AAGAAAGACT GGGACTGACT 
GAGATCAGGA AACACGCCAA CAGAATGACG TTTGCTGAGA TTGAGGACGA TGCCTATCAG 
GAGGATCTGG GCTTCAGTCT CGGTCAGCTG GGAAAATCTG GCAGTGGCAG AGTCAGACAA 
GCTCAGGTCA ACGACTCCAC CAAAGCCAGA ATATCCAAAT CTTTACAGAG GACACTGCAG 
AAGCAGAGCA TGACGTACGG AGGCAAGTCT ACAGTGAGAG ACCGATCGTC TGGAACCAGC 
TCCAGCGTGG CCTTCACTCC TCTACAGGGG CTGGAGATTG TGAACCCACA GGCTGCAGAG 
AAGAAGGTGG CTGAAGCCAA TCAGAAATAT TTCTCCAACA TGGCTGAGTT CCTCAAGGTC 
AAGCGTGAGA AGGAGGACAA GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956798.1
CDS	113..1639
Translation	MSLADELLADLEEAGEEDGLYPGGEEGESDGEPGERQVDGGLEDIPEEMEVDYSSTESVTSIAKLRHSKPFAEIMDKISHYVGNQRKNSEVSGPVEADPEYRLIVAANNLTVEIDNELNIIHKFVRDKYSKRFPELESLVPNALDYIRTVKELGNNLEKCKNNETLQQILTNATIMVVSVTASTTQGTMLGDDELQRLEEACDMALELNQSKHRIYEYVESRMSFIAPNLSIIVGASTAAKIMGVAGGLTNLSKMPACNLMLLGAQRRTLSGFSSTSLLPHTGYIYHCDVVQTLPPDLRRKAARLVSAKCTLASRVDSFHESADGKVGYDLKEEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGGRRYRKMKERLGLTEIRKHANRMTFAEIEDDAYQEDLGFSLGQLGKSGSGRVRQAQVNDSTKARISKSLQRTLQKQSMTYGGKSTVRDRSSGTSSSVAFTPLQGLEIVNPQAAEKKVAEANQKYFSNMAEFLKVKREKEDKV

Target ORF information:

RefSeq Version	NM_200504.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio PRP31 pre-mRNA processing factor 31 homolog (yeast) (prpf31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200504.2

ORF Insert Sequence:

ATGTCTTTGG CAGACGAGCT GCTTGCGGAT TTGGAGGAGG CAGGAGAAGA GGACGGGCTG 
TATCCCGGCG GAGAAGAGGG AGAGAGTGAT GGAGAGCCCG GAGAGAGACA GGTTGATGGA 
GGACTGGAGG ACATCCCGGA GGAGATGGAG GTGGATTACA GCAGCACGGA GAGCGTCACG 
TCCATAGCTA AACTACGACA CAGCAAACCG TTCGCTGAAA TCATGGATAA AATCAGTCAC 
TATGTTGGAA ATCAACGCAA GAATTCAGAA GTGTCTGGAC CGGTTGAAGC AGACCCAGAG 
TACAGGCTCA TTGTTGCCGC CAACAATCTG ACGGTGGAAA TAGACAACGA GCTTAATATC 
ATTCACAAGT TTGTTCGTGA CAAGTACTCC AAAAGGTTTC CTGAGCTGGA GTCTCTGGTG 
CCAAATGCAC TGGACTACAT CAGAACTGTT AAGGAGCTGG GCAACAATCT GGAAAAGTGC 
AAAAACAATG AGACCCTTCA GCAGATTCTC ACCAATGCCA CCATTATGGT GGTCAGCGTC 
ACAGCCTCTA CCACACAAGG AACGATGCTT GGAGATGATG AGCTCCAGCG ATTGGAGGAG 
GCCTGTGACA TGGCCCTTGA GCTCAACCAA TCAAAACACA GGATCTATGA ATATGTGGAA 
TCCAGAATGT CATTCATTGC TCCGAATCTT TCAATTATTG TCGGAGCTTC AACTGCTGCA 
AAGATCATGG GTGTTGCTGG TGGTCTGACT AACCTGTCCA AGATGCCCGC ATGTAATCTG 
ATGTTGCTGG GAGCGCAGAG GAGAACACTG TCTGGATTCA GCAGCACATC TCTGCTGCCC 
CACACAGGCT ACATCTACCA CTGTGATGTA GTGCAGACTC TGCCTCCAGA TCTAAGGAGG 
AAAGCAGCTC GTCTGGTTTC TGCTAAATGC ACTCTGGCTT CTCGCGTGGA CAGTTTTCAT 
GAGAGCGCAG ATGGCAAGGT TGGCTATGAT TTGAAGGAAG AGATTGAGAG GAAGTTTGAT 
AAATGGCAGG AGCCTCCACC AGTCAAGCAG GTCAAGCCTC TTCCAGCTCC TCTGGATGGA 
CAGAGGAAGA AGAGAGGAGG AAGAAGGTAT CGTAAGATGA AAGAAAGACT GGGACTGACT 
GAGATCAGGA AACACGCCAA CAGAATGACG TTTGCTGAGA TTGAGGACGA TGCCTATCAG 
GAGGATCTGG GCTTCAGTCT CGGTCAGCTG GGAAAATCTG GCAGTGGCAG AGTCAGACAA 
GCTCAGGTCA ACGACTCCAC CAAAGCCAGA ATATCCAAAT CTTTACAGAG GACACTGCAG 
AAGCAGAGCA TGACGTACGG AGGCAAGTCT ACAGTGAGAG ACCGATCGTC TGGAACCAGC 
TCCAGCGTGG CCTTCACTCC TCTACAGGGG CTGGAGATTG TGAACCCACA GGCTGCAGAG 
AAGAAGGTGG CTGAAGCCAA TCAGAAATAT TTCTCCAACA TGGCTGAGTT CCTCAAGGTC 
AAGCGTGAGA AGGAGGACAA GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Knocking Down Snrnp200 Initiates Demorphogenesis of Rod Photoreceptors in Zebrafish.
Journal of ophthalmology2015816329(2015)
Liu Y,Chen X,Qin B,Zhao K,Zhao Q,Staley JP,Zhao C

PRPF4 mutations cause autosomal dominant retinitis pigmentosa.
Human molecular genetics23(11)2926-39(2014 Jun)
Chen X,Liu Y,Sheng X,Tam PO,Zhao K,Chen X,Rong W,Liu Y,Liu X,Pan X,Chen LJ,Zhao Q,Vollrath D,Pang CP,Zhao C

Mutant Prpf31 causes pre-mRNA splicing defects and rod photoreceptor cell degeneration in a zebrafish model for Retinitis pigmentosa.
Molecular neurodegeneration656(2011 Jul)
Yin J,Brocher J,Fischer U,Winkler C

Systemic splicing factor deficiency causes tissue-specific defects: a zebrafish model for retinitis pigmentosa.
Human molecular genetics20(2)368-77(2011 Jan)
Linder B,Dill H,Hirmer A,Brocher J,Lee GP,Mathavan S,Bolz HJ,Winkler C,Laggerbauer B,Fischer U

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prpf31 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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