Toe1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Toe1
Entrez Gene ID	68276
Full Name	target of EGR1, member 1 (nuclear)
Synonyms	4930584N22Rik,4933424D16Rik,AI413517
General protein information	Preferred Names target of EGR1, member 1 (nuclear) Names target of EGR1 protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_026654.2	NP_080930.1	target of EGR1 protein 1
XM_006503353.4	XP_006503416.1	target of EGR1 protein 1 isoform X1
XM_006503353.3	XP_006503416.1	target of EGR1 protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TOE1	XP_513124.3
Canis lupus familiaris (dog)	TOE1	XP_003639572.1
Gallus gallus (chicken)	TOE1	XP_422434.2
Rattus norvegicus (Norway rat)	Toe1	NP_001100150.1
Danio rerio (zebrafish)	toe1	NP_001243611.1
	TOE1	XP_001101649.2
Bos taurus (cattle)	TOE1	NP_001069062.1
Mus musculus (house mouse)	Toe1	NP_080930.1
Caenorhabditis elegans (roundworm)	CELE_Y57A10A.25	NP_496602.1
Xenopus tropicalis (tropical clawed frog)	toe1	NP_001120127.1
Homo sapiens (human)	TOE1	NP_079353.3

Gene Ontology
Bibliography

Related articles in PubMed

Biallelic mutations in the 3' exonuclease TOE1 cause pontocerebellar hypoplasia and uncover a role in snRNA processing.
Lardelli RM, Schaffer AE, Eggens VR, Zaki MS, Grainger S, Sathe S, Van Nostrand EL, Schlachetzki Z, Rosti B, Akizu N, Scott E, Silhavy JL, Heckman LD, Rosti RO, Dikoglu E, Gregor A, Guemez-Gamboa A, Musaev D, Mande R, Widjaja A, Shaw TL, Markmiller S, Marin-Valencia I, Davies JH, de Meirleir L, Kayserili H, Altunoglu U, Freckmann ML, Warwick L, Chitayat D, Blaser S, ?a?layan AO, Bilguvar K, Per H, Fagerberg C, Christesen HT, Kibaek M, Aldinger KA, Manchester D, Matsumoto N, Muramatsu K, Saitsu H, Shiina M, Ogata K, Foulds N, Dobyns WB, Chi NC, Traver D, Spaccini L, Bova SM, Gabriel SB, Gunel M, Valente EM, Nassogne MC, Bennett EJ, Yeo GW, Baas F, Lykke-Andersen J, Gleeson JG
Nature genetics49(3)457-464(2017 Mar)

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Guo G, Huss M, Tong GQ, Wang C, Li Sun L, Clarke ND, Robson P
Developmental cell18(4)675-85(2010 Apr)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Toe1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Toe1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02753	NM_026654.2 Latest version!	Mus musculus target of EGR1, member 1 (nuclear) (Toe1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu33779	XM_006503353.4 Latest version!	Mus musculus target of EGR1, member 1 (nuclear) (Toe1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu33779	XM_006503353.3	Mus musculus target of EGR1, member 1 (nuclear) (Toe1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Toe1 ( NM_026654.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080930.1 Mus musculus target of EGR1, member 1 (nuclear) (Toe1), mRNA.

Price & Availability↑

CloneID	OMu02753
Clone ID Related Accession (Same CDS sequence)	NM_026654.2
Accession Version	NM_026654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	target of EGR1 protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL683847.12. On Nov 17, 2006 this sequence version replaced NM_026654.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK019829.1, AK082975.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164141 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCGG ACAGTGACGA TGATGTGCCT CCAGTTCCAA CGCCCTCTGA CGGTGGTGTC 
AACAAAAACA CACAACCTGC AGAGGAGTTT GTGGTTCGGG TTCCTGTGGT AGACGTGCAG 
AGTGACAACT TCAAGGAGAT ATGGCCATCA CTCTTGCTAG CCTTAAAGAC AGCTAGCTTT 
GTGGCTGTGG ACACGGAGCT GAGTGGGCTC GGGGATAGGA AGAGTTTGCT GAACCAGTGC 
ATTGAGGAAC GTTACAAGGC CGTGTGTCAT GCTGCCAGGA CCCGTTCTGT CCTGTCCCTG 
GGCCTCGCCT GCTTCAGGCA GCAACCAGAT AAGGGTGAAA ATTCTTACTT GGCTCAAGTG 
TTCAATCTCA CCCTGCTATG CATAGAGGAG TACGTTATAG AACCGAAGTC TGTGCAGTTC 
CTGGTACAGC ATGGCTTCAA CTTCAACCGG CAGTACGCCC AGGGTATCCC CTACCATAAG 
GGCAATGACA AGGGAGATGA GAGCCAGAGC CAGTCGGTAC GGACGCTGTT CCTTGAACTG 
ATTCGGGCCC GACGGCCCCT GGTGCTACAT AATGGCCTTA TAGACTTGGT GTTCCTGTAC 
CAGAATTTCT ACGCGCACCT GCCTGAGAAC TTGGGGACCT TCACTGCTGA CCTGTGTGAG 
ATGTTCCCAG CAGGCATCTA TGACACCAAA TATGCCGCCG AGTTCCATGC CCGATTTGTA 
GCTTCCTACT TAGAATACGC TTTCCGGAAA TGTGAGCGGG AGAATGGGAA GCAGCGGGCA 
GCTGGCAGCC CACATCTTGC CCTGGAATTC TGCAGCTATC CATCTAGCAT GAGCGGGCAC 
ATTGACTACC GCTGCTGTAT GTCCCCTGGG ACCTGCCGTC GTTCTCGTAC CACTGGCATC 
TGTGACAAGT TCTCGGCCTA TGGCTGGTGT CCTCTGGGAC CACAGTGTCC TCAGTCACAC 
GATATTGACC TTATAATTGA CACTGATGAG GCTGTGGCAG AAGATAAGCG GCGGAGACGG 
CGACGTAAGG ACAAACGGAA GAGGTCTTTA CAGAGCCAAC CAGGGACACA GACCCTTGCG 
GAAGCTGAAG ATGGTCCTCC CACAAAACAG GTCTGTGAAG ATAGCCTCGA GACTGAGAAA 
ATGGAACAAA AAGTGGCAGA AGGTGAGGCT GGGGATCAGC CTGGCTCCAG GGAAGGTCAC 
ACAAGTAGCT TGGAGATGGC ACATAGGAGG ACAAGTGCTG AAACAGCTGA TGTGGCCACC 
TCAGAGCTGC TGGTGAACCA AGCCAGCACT AACCCCGTGC CTGGGGACGG ACTGCATCGT 
GCTGGTTTTG ACGCCTTTAT GACAGGCTAT GTGATGGCCT ATGTGGGCCT GAGCCAGGGA 
CTTCAACTCT GCAGCTCTGA ACCTTGGCTT CCTGAATGTC ACAACAAGGT ATATCTAAGT 
GGCAAAACTG TACCCCTCAC AGTGACCAAG AGCCAGTTTT CCCGTCCATC CAAAGCCCAC 
AACCAGAAAA TGAAGCTGGC TTGGGGCAGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080930.1
CDS	41..1576
Translation	MAADSDDDVPPVPTPSDGGVNKNTQPAEEFVVRVPVVDVQSDNFKEIWPSLLLALKTASFVAVDTELSGLGDRKSLLNQCIEERYKAVCHAARTRSVLSLGLACFRQQPDKGENSYLAQVFNLTLLCIEEYVIEPKSVQFLVQHGFNFNRQYAQGIPYHKGNDKGDESQSQSVRTLFLELIRARRPLVLHNGLIDLVFLYQNFYAHLPENLGTFTADLCEMFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLALEFCSYPSSMSGHIDYRCCMSPGTCRRSRTTGICDKFSAYGWCPLGPQCPQSHDIDLIIDTDEAVAEDKRRRRRRKDKRKRSLQSQPGTQTLAEAEDGPPTKQVCEDSLETEKMEQKVAEGEAGDQPGSREGHTSSLEMAHRRTSAETADVATSELLVNQASTNPVPGDGLHRAGFDAFMTGYVMAYVGLSQGLQLCSSEPWLPECHNKVYLSGKTVPLTVTKSQFSRPSKAHNQKMKLAWGSS

Target ORF information:

RefSeq Version	NM_026654.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus target of EGR1, member 1 (nuclear) (Toe1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026654.2

ORF Insert Sequence:

ATGGCCGCGG ACAGTGACGA TGATGTGCCT CCAGTTCCAA CGCCCTCTGA CGGTGGTGTC 
AACAAAAACA CACAACCTGC AGAGGAGTTT GTGGTTCGGG TTCCTGTGGT AGACGTGCAG 
AGTGACAACT TCAAGGAGAT ATGGCCATCA CTCTTGCTAG CCTTAAAGAC AGCTAGCTTT 
GTGGCTGTGG ACACGGAGCT GAGTGGGCTC GGGGATAGGA AGAGTTTGCT GAACCAGTGC 
ATTGAGGAAC GTTACAAGGC CGTGTGTCAT GCTGCCAGGA CCCGTTCTGT CCTGTCCCTG 
GGCCTCGCCT GCTTCAGGCA GCAACCAGAT AAGGGTGAAA ATTCTTACTT GGCTCAAGTG 
TTCAATCTCA CCCTGCTATG CATAGAGGAG TACGTTATAG AACCGAAGTC TGTGCAGTTC 
CTGGTACAGC ATGGCTTCAA CTTCAACCGG CAGTACGCCC AGGGTATCCC CTACCATAAG 
GGCAATGACA AGGGAGATGA GAGCCAGAGC CAGTCGGTAC GGACGCTGTT CCTTGAACTG 
ATTCGGGCCC GACGGCCCCT GGTGCTACAT AATGGCCTTA TAGACTTGGT GTTCCTGTAC 
CAGAATTTCT ACGCGCACCT GCCTGAGAAC TTGGGGACCT TCACTGCTGA CCTGTGTGAG 
ATGTTCCCAG CAGGCATCTA TGACACCAAA TATGCCGCCG AGTTCCATGC CCGATTTGTA 
GCTTCCTACT TAGAATACGC TTTCCGGAAA TGTGAGCGGG AGAATGGGAA GCAGCGGGCA 
GCTGGCAGCC CACATCTTGC CCTGGAATTC TGCAGCTATC CATCTAGCAT GAGCGGGCAC 
ATTGACTACC GCTGCTGTAT GTCCCCTGGG ACCTGCCGTC GTTCTCGTAC CACTGGCATC 
TGTGACAAGT TCTCGGCCTA TGGCTGGTGT CCTCTGGGAC CACAGTGTCC TCAGTCACAC 
GATATTGACC TTATAATTGA CACTGATGAG GCTGTGGCAG AAGATAAGCG GCGGAGACGG 
CGACGTAAGG ACAAACGGAA GAGGTCTTTA CAGAGCCAAC CAGGGACACA GACCCTTGCG 
GAAGCTGAAG ATGGTCCTCC CACAAAACAG GTCTGTGAAG ATAGCCTCGA GACTGAGAAA 
ATGGAACAAA AAGTGGCAGA AGGTGAGGCT GGGGATCAGC CTGGCTCCAG GGAAGGTCAC 
ACAAGTAGCT TGGAGATGGC ACATAGGAGG ACAAGTGCTG AAACAGCTGA TGTGGCCACC 
TCAGAGCTGC TGGTGAACCA AGCCAGCACT AACCCCGTGC CTGGGGACGG ACTGCATCGT 
GCTGGTTTTG ACGCCTTTAT GACAGGCTAT GTGATGGCCT ATGTGGGCCT GAGCCAGGGA 
CTTCAACTCT GCAGCTCTGA ACCTTGGCTT CCTGAATGTC ACAACAAGGT ATATCTAAGT 
GGCAAAACTG TACCCCTCAC AGTGACCAAG AGCCAGTTTT CCCGTCCATC CAAAGCCCAC 
AACCAGAAAA TGAAGCTGGC TTGGGGCAGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Toe1 ( XM_006503353.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503416.1 Mus musculus target of EGR1, member 1 (nuclear) (Toe1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33779
Clone ID Related Accession (Same CDS sequence)	XM_006503353.4 , XM_006503353.3
Accession Version	XM_006503353.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	target of EGR1 protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAG CAGGCATCTA TGACACCAAA TATGCCGCCG AGTTCCATGC CCGATTTGTA 
GCTTCCTACT TAGAATACGC TTTCCGGAAA TGTGAGCGGG AGAATGGGAA GCAGCGGGCA 
GCTGGCAGCC CACATCTTGC CCTGGAATTC TGCAGCTATC CATCTAGCAT GAGCGGGCAC 
ATTGACTACC GCTGCTGTAT GTCCCCTGGG ACCTGCCGTC GTTCTCGTAC CACTGGCATC 
TGTGACAAGT TCTCGGCCTA TGGCTGGTGT CCTCTGGGAC CACAGTGTCC TCAGTCACAC 
GATATTGACC TTATAATTGA CACTGATGAG GCTGTGGCAG AAGATAAGCG GCGGAGACGG 
CGACGTAAGG ACAAACGGAA GAGGTCTTTA CAGAGCCAAC CAGGGACACA GACCCTTGCG 
GAAGCTGAAG ATGGTCCTCC CACAAAACAG GTCTGTGAAG ATAGCCTCGA GACTGAGAAA 
ATGGAACAAA AAGTGGCAGA AGGTGAGGCT GGGGATCAGC CTGGCTCCAG GGAAGGTCAC 
ACAAGTAGCT TGGAGATGGC ACATAGGAGG ACAAGTGCTG AAACAGCTGA TGTGGCCACC 
TCAGAGCTGC TGGTGAACCA AGCCAGCACT AACCCCGTGC CTGGGGACGG ACTGCATCGT 
GCTGGTTTTG ACGCCTTTAT GACAGGCTAT GTGATGGCCT ATGTGGGCCT GAGCCAGGGA 
CTTCAACTCT GCAGCTCTGA ACCTTGGCTT CCTGAATGTC ACAACAAGGT ATATCTAAGT 
GGCAAAACTG TACCCCTCAC AGTGACCAAG AGCCAGTTTT CCCGTCCATC CAAAGCCCAC 
AACCAGAAAA TGAAGCTGGC TTGGGGCAGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503416.1
CDS	798..1673
Translation	MFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLALEFCSYPSSMSGHIDYRCCMSPGTCRRSRTTGICDKFSAYGWCPLGPQCPQSHDIDLIIDTDEAVAEDKRRRRRRKDKRKRSLQSQPGTQTLAEAEDGPPTKQVCEDSLETEKMEQKVAEGEAGDQPGSREGHTSSLEMAHRRTSAETADVATSELLVNQASTNPVPGDGLHRAGFDAFMTGYVMAYVGLSQGLQLCSSEPWLPECHNKVYLSGKTVPLTVTKSQFSRPSKAHNQKMKLAWGSS

Target ORF information:

RefSeq Version	XM_006503353.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus target of EGR1, member 1 (nuclear) (Toe1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503353.3

ORF Insert Sequence:

ATGTTCCCAG CAGGCATCTA TGACACCAAA TATGCCGCCG AGTTCCATGC CCGATTTGTA 
GCTTCCTACT TAGAATACGC TTTCCGGAAA TGTGAGCGGG AGAATGGGAA GCAGCGGGCA 
GCTGGCAGCC CACATCTTGC CCTGGAATTC TGCAGCTATC CATCTAGCAT GAGCGGGCAC 
ATTGACTACC GCTGCTGTAT GTCCCCTGGG ACCTGCCGTC GTTCTCGTAC CACTGGCATC 
TGTGACAAGT TCTCGGCCTA TGGCTGGTGT CCTCTGGGAC CACAGTGTCC TCAGTCACAC 
GATATTGACC TTATAATTGA CACTGATGAG GCTGTGGCAG AAGATAAGCG GCGGAGACGG 
CGACGTAAGG ACAAACGGAA GAGGTCTTTA CAGAGCCAAC CAGGGACACA GACCCTTGCG 
GAAGCTGAAG ATGGTCCTCC CACAAAACAG GTCTGTGAAG ATAGCCTCGA GACTGAGAAA 
ATGGAACAAA AAGTGGCAGA AGGTGAGGCT GGGGATCAGC CTGGCTCCAG GGAAGGTCAC 
ACAAGTAGCT TGGAGATGGC ACATAGGAGG ACAAGTGCTG AAACAGCTGA TGTGGCCACC 
TCAGAGCTGC TGGTGAACCA AGCCAGCACT AACCCCGTGC CTGGGGACGG ACTGCATCGT 
GCTGGTTTTG ACGCCTTTAT GACAGGCTAT GTGATGGCCT ATGTGGGCCT GAGCCAGGGA 
CTTCAACTCT GCAGCTCTGA ACCTTGGCTT CCTGAATGTC ACAACAAGGT ATATCTAAGT 
GGCAAAACTG TACCCCTCAC AGTGACCAAG AGCCAGTTTT CCCGTCCATC CAAAGCCCAC 
AACCAGAAAA TGAAGCTGGC TTGGGGCAGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Toe1 ( XM_006503353.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503416.1 Mus musculus target of EGR1, member 1 (nuclear) (Toe1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33779
Clone ID Related Accession (Same CDS sequence)	XM_006503353.4 , XM_006503353.3
Accession Version	XM_006503353.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	target of EGR1 protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006503353.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAG CAGGCATCTA TGACACCAAA TATGCCGCCG AGTTCCATGC CCGATTTGTA 
GCTTCCTACT TAGAATACGC TTTCCGGAAA TGTGAGCGGG AGAATGGGAA GCAGCGGGCA 
GCTGGCAGCC CACATCTTGC CCTGGAATTC TGCAGCTATC CATCTAGCAT GAGCGGGCAC 
ATTGACTACC GCTGCTGTAT GTCCCCTGGG ACCTGCCGTC GTTCTCGTAC CACTGGCATC 
TGTGACAAGT TCTCGGCCTA TGGCTGGTGT CCTCTGGGAC CACAGTGTCC TCAGTCACAC 
GATATTGACC TTATAATTGA CACTGATGAG GCTGTGGCAG AAGATAAGCG GCGGAGACGG 
CGACGTAAGG ACAAACGGAA GAGGTCTTTA CAGAGCCAAC CAGGGACACA GACCCTTGCG 
GAAGCTGAAG ATGGTCCTCC CACAAAACAG GTCTGTGAAG ATAGCCTCGA GACTGAGAAA 
ATGGAACAAA AAGTGGCAGA AGGTGAGGCT GGGGATCAGC CTGGCTCCAG GGAAGGTCAC 
ACAAGTAGCT TGGAGATGGC ACATAGGAGG ACAAGTGCTG AAACAGCTGA TGTGGCCACC 
TCAGAGCTGC TGGTGAACCA AGCCAGCACT AACCCCGTGC CTGGGGACGG ACTGCATCGT 
GCTGGTTTTG ACGCCTTTAT GACAGGCTAT GTGATGGCCT ATGTGGGCCT GAGCCAGGGA 
CTTCAACTCT GCAGCTCTGA ACCTTGGCTT CCTGAATGTC ACAACAAGGT ATATCTAAGT 
GGCAAAACTG TACCCCTCAC AGTGACCAAG AGCCAGTTTT CCCGTCCATC CAAAGCCCAC 
AACCAGAAAA TGAAGCTGGC TTGGGGCAGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503416.1
CDS	788..1663
Translation	MFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLALEFCSYPSSMSGHIDYRCCMSPGTCRRSRTTGICDKFSAYGWCPLGPQCPQSHDIDLIIDTDEAVAEDKRRRRRRKDKRKRSLQSQPGTQTLAEAEDGPPTKQVCEDSLETEKMEQKVAEGEAGDQPGSREGHTSSLEMAHRRTSAETADVATSELLVNQASTNPVPGDGLHRAGFDAFMTGYVMAYVGLSQGLQLCSSEPWLPECHNKVYLSGKTVPLTVTKSQFSRPSKAHNQKMKLAWGSS

Target ORF information:

RefSeq Version	XM_006503353.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus target of EGR1, member 1 (nuclear) (Toe1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503353.4

ORF Insert Sequence:

ATGTTCCCAG CAGGCATCTA TGACACCAAA TATGCCGCCG AGTTCCATGC CCGATTTGTA 
GCTTCCTACT TAGAATACGC TTTCCGGAAA TGTGAGCGGG AGAATGGGAA GCAGCGGGCA 
GCTGGCAGCC CACATCTTGC CCTGGAATTC TGCAGCTATC CATCTAGCAT GAGCGGGCAC 
ATTGACTACC GCTGCTGTAT GTCCCCTGGG ACCTGCCGTC GTTCTCGTAC CACTGGCATC 
TGTGACAAGT TCTCGGCCTA TGGCTGGTGT CCTCTGGGAC CACAGTGTCC TCAGTCACAC 
GATATTGACC TTATAATTGA CACTGATGAG GCTGTGGCAG AAGATAAGCG GCGGAGACGG 
CGACGTAAGG ACAAACGGAA GAGGTCTTTA CAGAGCCAAC CAGGGACACA GACCCTTGCG 
GAAGCTGAAG ATGGTCCTCC CACAAAACAG GTCTGTGAAG ATAGCCTCGA GACTGAGAAA 
ATGGAACAAA AAGTGGCAGA AGGTGAGGCT GGGGATCAGC CTGGCTCCAG GGAAGGTCAC 
ACAAGTAGCT TGGAGATGGC ACATAGGAGG ACAAGTGCTG AAACAGCTGA TGTGGCCACC 
TCAGAGCTGC TGGTGAACCA AGCCAGCACT AACCCCGTGC CTGGGGACGG ACTGCATCGT 
GCTGGTTTTG ACGCCTTTAT GACAGGCTAT GTGATGGCCT ATGTGGGCCT GAGCCAGGGA 
CTTCAACTCT GCAGCTCTGA ACCTTGGCTT CCTGAATGTC ACAACAAGGT ATATCTAAGT 
GGCAAAACTG TACCCCTCAC AGTGACCAAG AGCCAGTTTT CCCGTCCATC CAAAGCCCAC 
AACCAGAAAA TGAAGCTGGC TTGGGGCAGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biallelic mutations in the 3' exonuclease TOE1 cause pontocerebellar hypoplasia and uncover a role in snRNA processing.
Nature genetics49(3)457-464(2017 Mar)
Lardelli RM,Schaffer AE,Eggens VR,Zaki MS,Grainger S,Sathe S,Van Nostrand EL,Schlachetzki Z,Rosti B,Akizu N,Scott E,Silhavy JL,Heckman LD,Rosti RO,Dikoglu E,Gregor A,Guemez-Gamboa A,Musaev D,Mande R,Widjaja A,Shaw TL,Markmiller S,Marin-Valencia I,Davies JH,de Meirleir L,Kayserili H,Altunoglu U,Freckmann ML,Warwick L,Chitayat D,Blaser S,?a?layan AO,Bilguvar K,Per H,Fagerberg C,Christesen HT,Kibaek M,Aldinger KA,Manchester D,Matsumoto N,Muramatsu K,Saitsu H,Shiina M,Ogata K,Foulds N,Dobyns WB,Chi NC,Traver D,Spaccini L,Bova SM,Gabriel SB,Gunel M,Valente EM,Nassogne MC,Bennett EJ,Yeo GW,Baas F,Lykke-Andersen J,Gleeson JG

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Developmental cell18(4)675-85(2010 Apr)
Guo G,Huss M,Tong GQ,Wang C,Li Sun L,Clarke ND,Robson P

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

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Toe1 cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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