ORF » Species Summary » Danio rerio » toe1 cDNA ORF clone

toe1 cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following toe1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the toe1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa34113 NM_001256682.1
Latest version!		 Danio rerio target of EGR1, exonuclease (toe1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    toe1 ( NM_001256682.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001243611.1 Danio rerio target of EGR1, exonuclease (toe1), mRNA.
    Price & Availability↑
    CloneID ODa34113
    Clone ID Related Accession (Same CDS sequence) NM_001256682.1
    Accession Version NM_001256682.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product target of EGR1 protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU571172.7 and BX649522.15. On Feb 17, 2012 this sequence version replaced XM_001923878.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01041656.1, GDQH01015637.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTCGTCCT CAATGATAAT CCCAGTGATT GATGTGCAGG GAAACAATTT TAAGGAGTTG 
    TGGGCGGCGA TGGTGATAGC CATTAAAACG TCTTCATTCA TCGCCATTGA TACGGAACTG 
    AGTGGTCTTG GTTCAAGGAA AGCTCTTCTA GCTGAGTCGA TTGAAGACAG GTACAATGCC 
    ATATGCCATG CTGCCCGCAC CCGATCTGTA CTGTCTTTGG GGTTTGCCTG TTATAAGGAG 
    CTTGAAAACA AAGCCGAGAC CACTTACCTG GTCCAGGTTT ACAATCTTAC CTTGCTTTGC 
    TCGGAGGAAT ACATCATTGA ACCGCAGTCA GTGCAGTTCC TTGTGCAACA TGGATTTGAC 
    TTCAACAAAC AGTATGCTGA AGGCATTCCT TATTATAAAG GGAATGACAA AGGAGGCGAT 
    GCCCATGGGG TAAACATACG GTCATTATTT GTCGAACTTC TCAGAGCCAA CAAGCCCCTG 
    GTGCTCCATA ACGGACTGAT AGACCTGGTC TTCTTGTATC AGTGTTTCTA TGCCCATCTG 
    CCTGATCGTC TGGGCACCTT CACTGCCGAT CTCTCCCAGA TGTTTCCCTC AGGAATCTAC 
    GACACAAAGT ATGCAACAGA GTATGAGCTG AGGTTTACCG CCTCCTACTT GGAGTATGCC 
    TATAAGAAGT GTAAGCTGGA AAATAGCAAA GCCATTGAAG CAGAAGGCAG TGGGAGTAAC 
    CTGTTCGTGG AGTTTTGTAA ATACACAGGC AACATGGAGA CCTACATAGA CTACAGGCCT 
    TGCTTGGACA GCCAGAGTCA GGATGATGGA GCCCTCAACA TATGTGTTCA GTTCTCCGCT 
    TATGGATGGT GTCCTAATGG CTCCCAGTGC CCCATGTCAC ACAACACGGA CCTTATAATC 
    CAGCAAGATG AGAAGACCAG AGAAGACAAG AGGAAGAAGC GGAAGCGCAA GAACAAAAAG 
    AAAGGCTCTC AGGGACCCCC AGAAACATGC AGTTCCCCGC AGGGCAAAAG AACACACTTT 
    GAAGAAGCCG AGCTGGACCA GGCTGTGCCC ACATCTGAAC ATCAAGAGAA GACCCTGAAT 
    GCTGAGCCAA CAGACGAACC TGGAAAAACA GCCAGCAACA ATGGAGATGA GCAATCCCAG 
    CCAGAAGCAT CTACTGAAGC TCAGGAGAAA CCAAAGGGGA AGAAAGTGGA AGGGGGTACT 
    CACAGAGCAG GGTTCGACGC TTTCATGACA GGGTATATTT TTGCGTATGC TAGAAATTTG 
    ATGAAAAACA AAGAAGAATC CAGCAGCACT CCTGTCATTT CTACATGTCT AAATAAGCTG 
    TTTCTCAGCG GCAAGTCTGT TCCTCTGCAT GTGGCCAAAA GCACCTTTTC CAAATCTTCA 
    AAGGCTCATG TGCAGAAGAT GGCCTATGTC TGGGGGAAAA GCATTGATGT AAACATGGAA 
    AAAAGTGCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243611.1
    CDS330..1781
    Translation

    Target ORF information:

    RefSeq Version NM_001256682.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio target of EGR1, exonuclease (toe1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001256682.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTCGTCCT CAATGATAAT CCCAGTGATT GATGTGCAGG GAAACAATTT TAAGGAGTTG 
    TGGGCGGCGA TGGTGATAGC CATTAAAACG TCTTCATTCA TCGCCATTGA TACGGAACTG 
    AGTGGTCTTG GTTCAAGGAA AGCTCTTCTA GCTGAGTCGA TTGAAGACAG GTACAATGCC 
    ATATGCCATG CTGCCCGCAC CCGATCTGTA CTGTCTTTGG GGTTTGCCTG TTATAAGGAG 
    CTTGAAAACA AAGCCGAGAC CACTTACCTG GTCCAGGTTT ACAATCTTAC CTTGCTTTGC 
    TCGGAGGAAT ACATCATTGA ACCGCAGTCA GTGCAGTTCC TTGTGCAACA TGGATTTGAC 
    TTCAACAAAC AGTATGCTGA AGGCATTCCT TATTATAAAG GGAATGACAA AGGAGGCGAT 
    GCCCATGGGG TAAACATACG GTCATTATTT GTCGAACTTC TCAGAGCCAA CAAGCCCCTG 
    GTGCTCCATA ACGGACTGAT AGACCTGGTC TTCTTGTATC AGTGTTTCTA TGCCCATCTG 
    CCTGATCGTC TGGGCACCTT CACTGCCGAT CTCTCCCAGA TGTTTCCCTC AGGAATCTAC 
    GACACAAAGT ATGCAACAGA GTATGAGCTG AGGTTTACCG CCTCCTACTT GGAGTATGCC 
    TATAAGAAGT GTAAGCTGGA AAATAGCAAA GCCATTGAAG CAGAAGGCAG TGGGAGTAAC 
    CTGTTCGTGG AGTTTTGTAA ATACACAGGC AACATGGAGA CCTACATAGA CTACAGGCCT 
    TGCTTGGACA GCCAGAGTCA GGATGATGGA GCCCTCAACA TATGTGTTCA GTTCTCCGCT 
    TATGGATGGT GTCCTAATGG CTCCCAGTGC CCCATGTCAC ACAACACGGA CCTTATAATC 
    CAGCAAGATG AGAAGACCAG AGAAGACAAG AGGAAGAAGC GGAAGCGCAA GAACAAAAAG 
    AAAGGCTCTC AGGGACCCCC AGAAACATGC AGTTCCCCGC AGGGCAAAAG AACACACTTT 
    GAAGAAGCCG AGCTGGACCA GGCTGTGCCC ACATCTGAAC ATCAAGAGAA GACCCTGAAT 
    GCTGAGCCAA CAGACGAACC TGGAAAAACA GCCAGCAACA ATGGAGATGA GCAATCCCAG 
    CCAGAAGCAT CTACTGAAGC TCAGGAGAAA CCAAAGGGGA AGAAAGTGGA AGGGGGTACT 
    CACAGAGCAG GGTTCGACGC TTTCATGACA GGGTATATTT TTGCGTATGC TAGAAATTTG 
    ATGAAAAACA AAGAAGAATC CAGCAGCACT CCTGTCATTT CTACATGTCT AAATAAGCTG 
    TTTCTCAGCG GCAAGTCTGT TCCTCTGCAT GTGGCCAAAA GCACCTTTTC CAAATCTTCA 
    AAGGCTCATG TGCAGAAGAT GGCCTATGTC TGGGGGAAAA GCATTGATGT AAACATGGAA 
    AAAAGTGCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Biallelic mutations in the 3' exonuclease TOE1 cause pontocerebellar hypoplasia and uncover a role in snRNA processing.
    Nature genetics49(3)457-464(2017 Mar)
    Lardelli RM,Schaffer AE,Eggens VR,Zaki MS,Grainger S,Sathe S,Van Nostrand EL,Schlachetzki Z,Rosti B,Akizu N,Scott E,Silhavy JL,Heckman LD,Rosti RO,Dikoglu E,Gregor A,Guemez-Gamboa A,Musaev D,Mande R,Widjaja A,Shaw TL,Markmiller S,Marin-Valencia I,Davies JH,de Meirleir L,Kayserili H,Altunoglu U,Freckmann ML,Warwick L,Chitayat D,Blaser S,?a?layan AO,Bilguvar K,Per H,Fagerberg C,Christesen HT,Kibaek M,Aldinger KA,Manchester D,Matsumoto N,Muramatsu K,Saitsu H,Shiina M,Ogata K,Foulds N,Dobyns WB,Chi NC,Traver D,Spaccini L,Bova SM,Gabriel SB,Gunel M,Valente EM,Nassogne MC,Bennett EJ,Yeo GW,Baas F,Lykke-Andersen J,Gleeson JG


    RFX transcription factors are essential for hearing in mice.
    Nature communications68549(2015 Oct)
    Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R


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