Pomgnt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pomgnt1
Entrez Gene ID	68273
Full Name	protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase
Synonyms	0610016I07Rik,4930467B06Rik
General protein information	Preferred Names protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase Names protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 O-mannosyl N-acetylglucosaminyltransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_029786.2	NP_084062.2	protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 isoform 2
NM_026651.2	NP_080927.1	protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 isoform 1
NM_001290658.1	NP_001277587.1	protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 isoform 3
XM_006503352.1	XP_006503415.1	protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pomgnt1	NP_080927.1
Gallus gallus (chicken)	POMGNT1	XP_426653.3
Danio rerio (zebrafish)	pomgnt1	NP_001036152.3
Bos taurus (cattle)	POMGNT1	NP_001019656.2
Rattus norvegicus (Norway rat)	Pomgnt1	NP_001007748.1
Xenopus tropicalis (tropical clawed frog)	pomgnt1	NP_001121493.1
Canis lupus familiaris (dog)	POMGNT1	XP_861245.1
Homo sapiens (human)	POMGNT1	NP_060209.3

Gene Ontology
Bibliography

Related articles in PubMed

Degree of Cajal-Retzius Cell Mislocalization Correlates with the Severity of Structural Brain Defects in Mouse Models of Dystroglycanopathy.
Booler HS, Williams JL, Hopkinson M, Brown SC
Brain pathology (Zurich, Switzerland)26(4)465-78(2016 Jul)

Neurons and glia modify receptor protein-tyrosine phosphatase ? (RPTP?)/phosphacan with cell-specific O-mannosyl glycans in the developing brain.
Dwyer CA, Katoh T, Tiemeyer M, Matthews RT
The Journal of biological chemistry290(16)10256-73(2015 Apr)

Role of glycosyltransferase PomGnT1 in glioblastoma progression.
Lan J, Guo P, Lin Y, Mao Q, Guo L, Ge J, Li X, Jiang J, Lin X, Qiu Y
Neuro-oncology17(2)211-22(2015 Feb)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

AGO61-dependent GlcNAc modification primes the formation of functional glycans on ?-dystroglycan.
Yagi H, Nakagawa N, Saito T, Kiyonari H, Abe T, Toda T, Wu SW, Khoo KH, Oka S, Kato K
Scientific reports33288(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings suggest that PomGnT1 promotes GBM progression via activation of beta-catenin and may serve as a prognostic factor for glioma patient survival as well as a novel molecular target for anticancer therapy in malignant glioma.
Title: Role of glycosyltransferase PomGnT1 in glioblastoma progression.

POMGnT1 gene knockout cause basement membrane disruptions in a mouse model of dystroglycanopathy.
Title: Biochemical and biophysical changes underlie the mechanisms of basement membrane disruptions in a mouse model of dystroglycanopathy.

classical beta1,2-elongation and beta1,6-GlcNAc branching of O-mannose glycan structures are dependent upon the POMGnT1 enzyme and O-mannosylation is not limited solely to alpha-DG in the brain
Title: Glycomic analyses of mouse models of congenital muscular dystrophy.

Results describe the effect of altered glycosylation of alpha-dystroglycan caused by inactivation of POMGnT1 in the retina.
Title: Reactive gliosis of astrocytes and M?ller glial cells in retina of POMGnT1-deficient mice.

POMGnT1 knockout mice were viable but with reduced fertility and variable lifespan
Title: Cellular and molecular characterization of abnormal brain development in protein o-mannose N-acetylglucosaminyltransferase 1 knockout mice.

The following Pomgnt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pomgnt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18923	NM_029786.2 Latest version!	Mus musculus protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$370.30 $529.00
OMu19020	NM_026651.2 Latest version!	Mus musculus protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu20547	NM_001290658.1 Latest version!	Mus musculus protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu33778	XM_006503352.1 Latest version!	Mus musculus protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pomgnt1 ( NM_029786.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084062.2 Mus musculus protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18923
Clone ID Related Accession (Same CDS sequence)	NM_029786.2
Accession Version	NM_029786.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY732188.1, AK002638.1, AB053220.1 and AI848361.1. On Mar 21, 2009 this sequence version replaced NM_029786.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK002638.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGACT GGAAGCCCAG TCCCCTCATC AAGCCCTTTG GGGCTAGGAA GAAACGGAGC 
TGGTACCTTA CCTGGAAGTA TAAACTGACA AACCAGCGGG CCCTGAGGAG ATTCTGTCAG 
ACAGGGGCTG TGCTTTTCCT GCTGGTGACC GTCATTGTCA ACATCAAGTT GATCCTGGAC 
ACTCGGAGAG CAATCAGCGA GGCCAATGAA GACCCAGAAC CAGAGCAAGA CTATGATGAG 
GCCCTAGGAC GTCTGGAGTC CCCTCGGCGC AGAGGCAGCA GTCCCCGACG GGTGCTGGAT 
GTGGAAGTAT ACTCCAGTAG AAGCAAAGTG TATGTGGCTG TGGATGGCAC CACGGTGCTC 
GAGGATGAAG CCCGGGAGCA GGGCCGAGGC ATCCATGTCA TAGTCCTCAA CCAGGCCACG 
GGCCACGTGA TGGCAAAGCG CGTGTTTGAC ACATACTCTC CTCATGAGGA TGAGGCCATG 
GTGCTGTTCC TCAACATGGT GGCCCCCGGC CGCGTGCTGA TCTGCACGGT GAAGGATGAG 
GGTTCCTTCC ACCTCAAGGA CACAGCAAAG GCTCTGCTGA GGAGCCTGGG AAGTCAGGCA 
GGCCCTGCCC TTGGCTGGAG GGACACCTGG GCCTTCGTGG GACGAAAAGG AGAGGCTGAG 
TGTCACTGGG CAGATACGGA GTTGAACCGC CGCCGCAGGC GCTTCTGCAG CAAAGTGGAG 
GGCTACGGGA GCGTGTGCAG CTGCAAGGAC CCGACGCCTA TTGAGTTCAG CCCTGACCCA 
CTTCCAGACA ATAAGGTCCT CAATGTGCCT GTGGCTGTCA TTGCAGGGAA CCGGCCCAAT 
TACCTGTACA GGATGCTGCG CTCTCTGCTC TCAGCCCAAG GGGTATCTCC ACAGATGATA 
ACGGTCTTCA TTGATGGCTA CTATGAGGAG CCAATGGATG TGGTGGCGCT GTTTGGTCTG 
AGAGGCATCC AGCATACTCC TATCAGCATC AAGAACGCCC GTGTGTCTCA GCACTACAAG 
GCCAGCCTCA CTGCCACTTT CAACCTGTTT CCGGAGGCCA AGTTTGCTGT GGTTCTGGAA 
GAGGACCTGG ACATTGCCGT GGATTTTTTC AGTTTCCTGA GCCAGTCCAT CCACCTGCTG 
GAGGAAGATG ACAGCCTGTA CTGCATCTCT GCCTGGAATG ACCAGGGGTA TGAACACACA 
GCTGAGGATC CAGCACTACT GTACCGTGTG GAGACCATGC CTGGGCTAGG ATGGGTGCTC 
AGGAAATCTT TATACAAGGA GGAGCTTGAG CCCAAGTGGC CCACACCAGA AAAGCTGTGG 
GATTGGGACA TGTGGATGCG GATGCCGGAA CAGCGCCGGG GCCGAGAGTG TATCATCCCG 
GATGTTTCCC GATCCTACCA CTTTGGCATC GTCGGCCTCA ACATGAACGG CTACTTTCAT 
GAGGCCTACT TCAAGAAGCA CAAGTTCAAC ACAGTTCCAG GTGTCCAGCT CAGGAATGTA 
GACAGTCTGA AGAAAGAAGC TTATGAAGTG GAAATTCACA GACTGCTCAG TGAGGCCGAG 
GTTTTGGACC ACAGCAAGGA CCCTTGTGAA GACTCCTTCC TGCCAGACAC GGAGGGCCAC 
ACCTATGTGG CGTTTATCCG AATGGAGACA GATGATGACT TCGCCACCTG GACTCAGCTT 
GCCAAGTGCC TCCATATTTG GGACCTGGAT GTTCGTGGCA ACCACCGGGG CCTGTGGAGA 
TTATTTCGGA AGAAGAACCA CTTTTTGGTG GTGGGGGTCC CAGCCTCCCC TTACTCAGTG 
AAGAAGCCAC CATCAGTCAC CCCTATTTTC CTGGAGCCAC CCCCAAAGGA AGAAGGAGCC 
CCAGGGGCTG CTGAACAAAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084062.2
CDS	160..2043
Translation	MDDWKPSPLIKPFGARKKRSWYLTWKYKLTNQRALRRFCQTGAVLFLLVTVIVNIKLILDTRRAISEANEDPEPEQDYDEALGRLESPRRRGSSPRRVLDVEVYSSRSKVYVAVDGTTVLEDEAREQGRGIHVIVLNQATGHVMAKRVFDTYSPHEDEAMVLFLNMVAPGRVLICTVKDEGSFHLKDTAKALLRSLGSQAGPALGWRDTWAFVGRKGEAECHWADTELNRRRRRFCSKVEGYGSVCSCKDPTPIEFSPDPLPDNKVLNVPVAVIAGNRPNYLYRMLRSLLSAQGVSPQMITVFIDGYYEEPMDVVALFGLRGIQHTPISIKNARVSQHYKASLTATFNLFPEAKFAVVLEEDLDIAVDFFSFLSQSIHLLEEDDSLYCISAWNDQGYEHTAEDPALLYRVETMPGLGWVLRKSLYKEELEPKWPTPEKLWDWDMWMRMPEQRRGRECIIPDVSRSYHFGIVGLNMNGYFHEAYFKKHKFNTVPGVQLRNVDSLKKEAYEVEIHRLLSEAEVLDHSKDPCEDSFLPDTEGHTYVAFIRMETDDDFATWTQLAKCLHIWDLDVRGNHRGLWRLFRKKNHFLVVGVPASPYSVKKPPSVTPIFLEPPPKEEGAPGAAEQT

Target ORF information:

RefSeq Version	NM_029786.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029786.2

ORF Insert Sequence:

ATGGATGACT GGAAGCCCAG TCCCCTCATC AAGCCCTTTG GGGCTAGGAA GAAACGGAGC 
TGGTACCTTA CCTGGAAGTA TAAACTGACA AACCAGCGGG CCCTGAGGAG ATTCTGTCAG 
ACAGGGGCTG TGCTTTTCCT GCTGGTGACC GTCATTGTCA ACATCAAGTT GATCCTGGAC 
ACTCGGAGAG CAATCAGCGA GGCCAATGAA GACCCAGAAC CAGAGCAAGA CTATGATGAG 
GCCCTAGGAC GTCTGGAGTC CCCTCGGCGC AGAGGCAGCA GTCCCCGACG GGTGCTGGAT 
GTGGAAGTAT ACTCCAGTAG AAGCAAAGTG TATGTGGCTG TGGATGGCAC CACGGTGCTC 
GAGGATGAAG CCCGGGAGCA GGGCCGAGGC ATCCATGTCA TAGTCCTCAA CCAGGCCACG 
GGCCACGTGA TGGCAAAGCG CGTGTTTGAC ACATACTCTC CTCATGAGGA TGAGGCCATG 
GTGCTGTTCC TCAACATGGT GGCCCCCGGC CGCGTGCTGA TCTGCACGGT GAAGGATGAG 
GGTTCCTTCC ACCTCAAGGA CACAGCAAAG GCTCTGCTGA GGAGCCTGGG AAGTCAGGCA 
GGCCCTGCCC TTGGCTGGAG GGACACCTGG GCCTTCGTGG GACGAAAAGG AGAGGCTGAG 
TGTCACTGGG CAGATACGGA GTTGAACCGC CGCCGCAGGC GCTTCTGCAG CAAAGTGGAG 
GGCTACGGGA GCGTGTGCAG CTGCAAGGAC CCGACGCCTA TTGAGTTCAG CCCTGACCCA 
CTTCCAGACA ATAAGGTCCT CAATGTGCCT GTGGCTGTCA TTGCAGGGAA CCGGCCCAAT 
TACCTGTACA GGATGCTGCG CTCTCTGCTC TCAGCCCAAG GGGTATCTCC ACAGATGATA 
ACGGTCTTCA TTGATGGCTA CTATGAGGAG CCAATGGATG TGGTGGCGCT GTTTGGTCTG 
AGAGGCATCC AGCATACTCC TATCAGCATC AAGAACGCCC GTGTGTCTCA GCACTACAAG 
GCCAGCCTCA CTGCCACTTT CAACCTGTTT CCGGAGGCCA AGTTTGCTGT GGTTCTGGAA 
GAGGACCTGG ACATTGCCGT GGATTTTTTC AGTTTCCTGA GCCAGTCCAT CCACCTGCTG 
GAGGAAGATG ACAGCCTGTA CTGCATCTCT GCCTGGAATG ACCAGGGGTA TGAACACACA 
GCTGAGGATC CAGCACTACT GTACCGTGTG GAGACCATGC CTGGGCTAGG ATGGGTGCTC 
AGGAAATCTT TATACAAGGA GGAGCTTGAG CCCAAGTGGC CCACACCAGA AAAGCTGTGG 
GATTGGGACA TGTGGATGCG GATGCCGGAA CAGCGCCGGG GCCGAGAGTG TATCATCCCG 
GATGTTTCCC GATCCTACCA CTTTGGCATC GTCGGCCTCA ACATGAACGG CTACTTTCAT 
GAGGCCTACT TCAAGAAGCA CAAGTTCAAC ACAGTTCCAG GTGTCCAGCT CAGGAATGTA 
GACAGTCTGA AGAAAGAAGC TTATGAAGTG GAAATTCACA GACTGCTCAG TGAGGCCGAG 
GTTTTGGACC ACAGCAAGGA CCCTTGTGAA GACTCCTTCC TGCCAGACAC GGAGGGCCAC 
ACCTATGTGG CGTTTATCCG AATGGAGACA GATGATGACT TCGCCACCTG GACTCAGCTT 
GCCAAGTGCC TCCATATTTG GGACCTGGAT GTTCGTGGCA ACCACCGGGG CCTGTGGAGA 
TTATTTCGGA AGAAGAACCA CTTTTTGGTG GTGGGGGTCC CAGCCTCCCC TTACTCAGTG 
AAGAAGCCAC CATCAGTCAC CCCTATTTTC CTGGAGCCAC CCCCAAAGGA AGAAGGAGCC 
CCAGGGGCTG CTGAACAAAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pomgnt1 ( NM_026651.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080927.1 Mus musculus protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19020
Clone ID Related Accession (Same CDS sequence)	NM_026651.2
Accession Version	NM_026651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY732188.1, AB053220.1 and AI848361.1. On Mar 21, 2009 this sequence version replaced NM_026651.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB053220.1, SRR1660817.5367.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGACT GGAAGCCCAG TCCCCTCATC AAGCCCTTTG GGGCTAGGAA GAAACGGAGC 
TGGTACCTTA CCTGGAAGTA TAAACTGACA AACCAGCGGG CCCTGAGGAG ATTCTGTCAG 
ACAGGGGCTG TGCTTTTCCT GCTGGTGACC GTCATTGTCA ACATCAAGTT GATCCTGGAC 
ACTCGGAGAG CAATCAGCGA GGCCAATGAA GACCCAGAAC CAGAGCAAGA CTATGATGAG 
GCCCTAGGAC GTCTGGAGTC CCCTCGGCGC AGAGGCAGCA GTCCCCGACG GGTGCTGGAT 
GTGGAAGTAT ACTCCAGTAG AAGCAAAGTG TATGTGGCTG TGGATGGCAC CACGGTGCTC 
GAGGATGAAG CCCGGGAGCA GGGCCGAGGC ATCCATGTCA TAGTCCTCAA CCAGGCCACG 
GGCCACGTGA TGGCAAAGCG CGTGTTTGAC ACATACTCTC CTCATGAGGA TGAGGCCATG 
GTGCTGTTCC TCAACATGGT GGCCCCCGGC CGCGTGCTGA TCTGCACGGT GAAGGATGAG 
GGTTCCTTCC ACCTCAAGGA CACAGCAAAG GCTCTGCTGA GGAGCCTGGG AAGTCAGGCA 
GGCCCTGCCC TTGGCTGGAG GGACACCTGG GCCTTCGTGG GACGAAAAGG AGGTCCTGTA 
CTTGGCGAGA AACATTCTAA ATCTCCTGCC CTCTCCTCCT GGGGTGACCC AGTCCTGCTG 
AAGACAGATG TGCCACTGAG CTCAGCTGAA GAGGCTGAGT GTCACTGGGC AGATACGGAG 
TTGAACCGCC GCCGCAGGCG CTTCTGCAGC AAAGTGGAGG GCTACGGGAG CGTGTGCAGC 
TGCAAGGACC CGACGCCTAT TGAGTTCAGC CCTGACCCAC TTCCAGACAA TAAGGTCCTC 
AATGTGCCTG TGGCTGTCAT TGCAGGGAAC CGGCCCAATT ACCTGTACAG GATGCTGCGC 
TCTCTGCTCT CAGCCCAAGG GGTATCTCCA CAGATGATAA CGGTCTTCAT TGATGGCTAC 
TATGAGGAGC CAATGGATGT GGTGGCGCTG TTTGGTCTGA GAGGCATCCA GCATACTCCT 
ATCAGCATCA AGAACGCCCG TGTGTCTCAG CACTACAAGG CCAGCCTCAC TGCCACTTTC 
AACCTGTTTC CGGAGGCCAA GTTTGCTGTG GTTCTGGAAG AGGACCTGGA CATTGCCGTG 
GATTTTTTCA GTTTCCTGAG CCAGTCCATC CACCTGCTGG AGGAAGATGA CAGCCTGTAC 
TGCATCTCTG CCTGGAATGA CCAGGGGTAT GAACACACAG CTGAGGATCC AGCACTACTG 
TACCGTGTGG AGACCATGCC TGGGCTAGGA TGGGTGCTCA GGAAATCTTT ATACAAGGAG 
GAGCTTGAGC CCAAGTGGCC CACACCAGAA AAGCTGTGGG ATTGGGACAT GTGGATGCGG 
ATGCCGGAAC AGCGCCGGGG CCGAGAGTGT ATCATCCCGG ATGTTTCCCG ATCCTACCAC 
TTTGGCATCG TCGGCCTCAA CATGAACGGC TACTTTCATG AGGCCTACTT CAAGAAGCAC 
AAGTTCAACA CAGTTCCAGG TGTCCAGCTC AGGAATGTAG ACAGTCTGAA GAAAGAAGCT 
TATGAAGTGG AAATTCACAG ACTGCTCAGT GAGGCCGAGG TTTTGGACCA CAGCAAGGAC 
CCTTGTGAAG ACTCCTTCCT GCCAGACACG GAGGGCCACA CCTATGTGGC GTTTATCCGA 
ATGGAGACAG ATGATGACTT CGCCACCTGG ACTCAGCTTG CCAAGTGCCT CCATATTTGG 
GACCTGGATG TTCGTGGCAA CCACCGGGGC CTGTGGAGAT TATTTCGGAA GAAGAACCAC 
TTTTTGGTGG TGGGGGTCCC AGCCTCCCCT TACTCAGTGA AGAAGCCACC ATCAGTCACC 
CCTATTTTCC TGGAGCCACC CCCAAAGGAA GAAGGAGCCC CAGGGGCTGC TGAACAAACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080927.1
CDS	160..2142
Translation	MDDWKPSPLIKPFGARKKRSWYLTWKYKLTNQRALRRFCQTGAVLFLLVTVIVNIKLILDTRRAISEANEDPEPEQDYDEALGRLESPRRRGSSPRRVLDVEVYSSRSKVYVAVDGTTVLEDEAREQGRGIHVIVLNQATGHVMAKRVFDTYSPHEDEAMVLFLNMVAPGRVLICTVKDEGSFHLKDTAKALLRSLGSQAGPALGWRDTWAFVGRKGGPVLGEKHSKSPALSSWGDPVLLKTDVPLSSAEEAECHWADTELNRRRRRFCSKVEGYGSVCSCKDPTPIEFSPDPLPDNKVLNVPVAVIAGNRPNYLYRMLRSLLSAQGVSPQMITVFIDGYYEEPMDVVALFGLRGIQHTPISIKNARVSQHYKASLTATFNLFPEAKFAVVLEEDLDIAVDFFSFLSQSIHLLEEDDSLYCISAWNDQGYEHTAEDPALLYRVETMPGLGWVLRKSLYKEELEPKWPTPEKLWDWDMWMRMPEQRRGRECIIPDVSRSYHFGIVGLNMNGYFHEAYFKKHKFNTVPGVQLRNVDSLKKEAYEVEIHRLLSEAEVLDHSKDPCEDSFLPDTEGHTYVAFIRMETDDDFATWTQLAKCLHIWDLDVRGNHRGLWRLFRKKNHFLVVGVPASPYSVKKPPSVTPIFLEPPPKEEGAPGAAEQT

Target ORF information:

RefSeq Version	NM_026651.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026651.2

ORF Insert Sequence:

ATGGATGACT GGAAGCCCAG TCCCCTCATC AAGCCCTTTG GGGCTAGGAA GAAACGGAGC 
TGGTACCTTA CCTGGAAGTA TAAACTGACA AACCAGCGGG CCCTGAGGAG ATTCTGTCAG 
ACAGGGGCTG TGCTTTTCCT GCTGGTGACC GTCATTGTCA ACATCAAGTT GATCCTGGAC 
ACTCGGAGAG CAATCAGCGA GGCCAATGAA GACCCAGAAC CAGAGCAAGA CTATGATGAG 
GCCCTAGGAC GTCTGGAGTC CCCTCGGCGC AGAGGCAGCA GTCCCCGACG GGTGCTGGAT 
GTGGAAGTAT ACTCCAGTAG AAGCAAAGTG TATGTGGCTG TGGATGGCAC CACGGTGCTC 
GAGGATGAAG CCCGGGAGCA GGGCCGAGGC ATCCATGTCA TAGTCCTCAA CCAGGCCACG 
GGCCACGTGA TGGCAAAGCG CGTGTTTGAC ACATACTCTC CTCATGAGGA TGAGGCCATG 
GTGCTGTTCC TCAACATGGT GGCCCCCGGC CGCGTGCTGA TCTGCACGGT GAAGGATGAG 
GGTTCCTTCC ACCTCAAGGA CACAGCAAAG GCTCTGCTGA GGAGCCTGGG AAGTCAGGCA 
GGCCCTGCCC TTGGCTGGAG GGACACCTGG GCCTTCGTGG GACGAAAAGG AGGTCCTGTA 
CTTGGCGAGA AACATTCTAA ATCTCCTGCC CTCTCCTCCT GGGGTGACCC AGTCCTGCTG 
AAGACAGATG TGCCACTGAG CTCAGCTGAA GAGGCTGAGT GTCACTGGGC AGATACGGAG 
TTGAACCGCC GCCGCAGGCG CTTCTGCAGC AAAGTGGAGG GCTACGGGAG CGTGTGCAGC 
TGCAAGGACC CGACGCCTAT TGAGTTCAGC CCTGACCCAC TTCCAGACAA TAAGGTCCTC 
AATGTGCCTG TGGCTGTCAT TGCAGGGAAC CGGCCCAATT ACCTGTACAG GATGCTGCGC 
TCTCTGCTCT CAGCCCAAGG GGTATCTCCA CAGATGATAA CGGTCTTCAT TGATGGCTAC 
TATGAGGAGC CAATGGATGT GGTGGCGCTG TTTGGTCTGA GAGGCATCCA GCATACTCCT 
ATCAGCATCA AGAACGCCCG TGTGTCTCAG CACTACAAGG CCAGCCTCAC TGCCACTTTC 
AACCTGTTTC CGGAGGCCAA GTTTGCTGTG GTTCTGGAAG AGGACCTGGA CATTGCCGTG 
GATTTTTTCA GTTTCCTGAG CCAGTCCATC CACCTGCTGG AGGAAGATGA CAGCCTGTAC 
TGCATCTCTG CCTGGAATGA CCAGGGGTAT GAACACACAG CTGAGGATCC AGCACTACTG 
TACCGTGTGG AGACCATGCC TGGGCTAGGA TGGGTGCTCA GGAAATCTTT ATACAAGGAG 
GAGCTTGAGC CCAAGTGGCC CACACCAGAA AAGCTGTGGG ATTGGGACAT GTGGATGCGG 
ATGCCGGAAC AGCGCCGGGG CCGAGAGTGT ATCATCCCGG ATGTTTCCCG ATCCTACCAC 
TTTGGCATCG TCGGCCTCAA CATGAACGGC TACTTTCATG AGGCCTACTT CAAGAAGCAC 
AAGTTCAACA CAGTTCCAGG TGTCCAGCTC AGGAATGTAG ACAGTCTGAA GAAAGAAGCT 
TATGAAGTGG AAATTCACAG ACTGCTCAGT GAGGCCGAGG TTTTGGACCA CAGCAAGGAC 
CCTTGTGAAG ACTCCTTCCT GCCAGACACG GAGGGCCACA CCTATGTGGC GTTTATCCGA 
ATGGAGACAG ATGATGACTT CGCCACCTGG ACTCAGCTTG CCAAGTGCCT CCATATTTGG 
GACCTGGATG TTCGTGGCAA CCACCGGGGC CTGTGGAGAT TATTTCGGAA GAAGAACCAC 
TTTTTGGTGG TGGGGGTCCC AGCCTCCCCT TACTCAGTGA AGAAGCCACC ATCAGTCACC 
CCTATTTTCC TGGAGCCACC CCCAAAGGAA GAAGGAGCCC CAGGGGCTGC TGAACAAACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pomgnt1 ( NM_001290658.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277587.1 Mus musculus protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant 3, mRNA.

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CloneID	OMu20547
Clone ID Related Accession (Same CDS sequence)	NM_001290658.1
Accession Version	NM_001290658.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK015478.1, AK019640.1 and AI848361.1. On Mar 15, 2014 this sequence version replaced XM_006503351.1. Transcript Variant: This variant (3) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 3, which has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK019640.1, AK015478.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGGA CCAAGGAAGT CAAGGACTCA AATGGGAAAA TACAAGACCA CGGGACAGGG 
GCTGTGCTTT TCCTGCTGGT GACCGTCATT GTCAACATCA AGTTGATCCT GGACACTCGG 
AGAGCAATCA GCGAGGCCAA TGAAGACCCA GAACCAGAGC AAGACTATGA TGAGGCCCTA 
GGACGTCTGG AGTCCCCTCG GCGCAGAGGC AGCAGTCCCC GACGGGTGCT GGATGTGGAA 
GTATACTCCA GTAGAAGCAA AGTGTATGTG GCTGTGGATG GCACCACGGT GCTCGAGGAT 
GAAGCCCGGG AGCAGGGCCG AGGCATCCAT GTCATAGTCC TCAACCAGGC CACGGGCCAC 
GTGATGGCAA AGCGCGTGTT TGACACATAC TCTCCTCATG AGGATGAGGC CATGGTGCTG 
TTCCTCAACA TGGTGGCCCC CGGCCGCGTG CTGATCTGCA CGGTGAAGGA TGAGGGTTCC 
TTCCACCTCA AGGACACAGC AAAGGCTCTG CTGAGGAGCC TGGGAAGTCA GGCAGGCCCT 
GCCCTTGGCT GGAGGGACAC CTGGGCCTTC GTGGGACGAA AAGGAGGTCC TGTACTTGGC 
GAGAAACATT CTAAATCTCC TGCCCTCTCC TCCTGGGGTG ACCCAGTCCT GCTGAAGACA 
GATGTGCCAC TGAGCTCAGC TGAAGAGGCT GAGTGTCACT GGGCAGATAC GGAGTTGAAC 
CGCCGCCGCA GGCGCTTCTG CAGCAAAGTG GAGGGCTACG GGAGCGTGTG CAGCTGCAAG 
GACCCGACGC CTATTGAGTT CAGCCCTGAC CCACTTCCAG ACAATAAGGT CCTCAATGTG 
CCTGTGGCTG TCATTGCAGG GAACCGGCCC AATTACCTGT ACAGGATGCT GCGCTCTCTG 
CTCTCAGCCC AAGGGGTATC TCCACAGATG ATAACGGTCT TCATTGATGG CTACTATGAG 
GAGCCAATGG ATGTGGTGGC GCTGTTTGGT CTGAGAGGCA TCCAGCATAC TCCTATCAGC 
ATCAAGAACG CCCGTGTGTC TCAGCACTAC AAGGCCAGCC TCACTGCCAC TTTCAACCTG 
TTTCCGGAGG CCAAGTTTGC TGTGGTTCTG GAAGAGGACC TGGACATTGC CGTGGATTTT 
TTCAGTTTCC TGAGCCAGTC CATCCACCTG CTGGAGGAAG ATGACAGCCT GTACTGCATC 
TCTGCCTGGA ATGACCAGGG GTATGAACAC ACAGCTGAGG ATCCAGCACT ACTGTACCGT 
GTGGAGACCA TGCCTGGGCT AGGATGGGTG CTCAGGAAAT CTTTATACAA GGAGGAGCTT 
GAGCCCAAGT GGCCCACACC AGAAAAGCTG TGGGATTGGG ACATGTGGAT GCGGATGCCG 
GAACAGCGCC GGGGCCGAGA GTGTATCATC CCGGATGTTT CCCGATCCTA CCACTTTGGC 
ATCGTCGGCC TCAACATGAA CGGCTACTTT CATGAGGCCT ACTTCAAGAA GCACAAGTTC 
AACACAGTTC CAGGTGTCCA GCTCAGGAAT GTAGACAGTC TGAAGAAAGA AGCTTATGAA 
GTGGAAATTC ACAGACTGCT CAGTGAGGCC GAGGTTTTGG ACCACAGCAA GGACCCTTGT 
GAAGACTCCT TCCTGCCAGA CACGGAGGGC CACACCTATG TGGCGTTTAT CCGAATGGAG 
ACAGATGATG ACTTCGCCAC CTGGACTCAG CTTGCCAAGT GCCTCCATAT TTGGGACCTG 
GATGTTCGTG GCAACCACCG GGGCCTGTGG AGATTATTTC GGAAGAAGAA CCACTTTTTG 
GTGGTGGGGG TCCCAGCCTC CCCTTACTCA GTGAAGAAGC CACCATCAGT CACCCCTATT 
TTCCTGGAGC CACCCCCAAA GGAAGAAGGA GCCCCAGGGG CTGCTGAACA AACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277587.1
CDS	140..2056
Translation	MEGTKEVKDSNGKIQDHGTGAVLFLLVTVIVNIKLILDTRRAISEANEDPEPEQDYDEALGRLESPRRRGSSPRRVLDVEVYSSRSKVYVAVDGTTVLEDEAREQGRGIHVIVLNQATGHVMAKRVFDTYSPHEDEAMVLFLNMVAPGRVLICTVKDEGSFHLKDTAKALLRSLGSQAGPALGWRDTWAFVGRKGGPVLGEKHSKSPALSSWGDPVLLKTDVPLSSAEEAECHWADTELNRRRRRFCSKVEGYGSVCSCKDPTPIEFSPDPLPDNKVLNVPVAVIAGNRPNYLYRMLRSLLSAQGVSPQMITVFIDGYYEEPMDVVALFGLRGIQHTPISIKNARVSQHYKASLTATFNLFPEAKFAVVLEEDLDIAVDFFSFLSQSIHLLEEDDSLYCISAWNDQGYEHTAEDPALLYRVETMPGLGWVLRKSLYKEELEPKWPTPEKLWDWDMWMRMPEQRRGRECIIPDVSRSYHFGIVGLNMNGYFHEAYFKKHKFNTVPGVQLRNVDSLKKEAYEVEIHRLLSEAEVLDHSKDPCEDSFLPDTEGHTYVAFIRMETDDDFATWTQLAKCLHIWDLDVRGNHRGLWRLFRKKNHFLVVGVPASPYSVKKPPSVTPIFLEPPPKEEGAPGAAEQT

Target ORF information:

RefSeq Version	NM_001290658.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290658.1

ORF Insert Sequence:

ATGGAGGGGA CCAAGGAAGT CAAGGACTCA AATGGGAAAA TACAAGACCA CGGGACAGGG 
GCTGTGCTTT TCCTGCTGGT GACCGTCATT GTCAACATCA AGTTGATCCT GGACACTCGG 
AGAGCAATCA GCGAGGCCAA TGAAGACCCA GAACCAGAGC AAGACTATGA TGAGGCCCTA 
GGACGTCTGG AGTCCCCTCG GCGCAGAGGC AGCAGTCCCC GACGGGTGCT GGATGTGGAA 
GTATACTCCA GTAGAAGCAA AGTGTATGTG GCTGTGGATG GCACCACGGT GCTCGAGGAT 
GAAGCCCGGG AGCAGGGCCG AGGCATCCAT GTCATAGTCC TCAACCAGGC CACGGGCCAC 
GTGATGGCAA AGCGCGTGTT TGACACATAC TCTCCTCATG AGGATGAGGC CATGGTGCTG 
TTCCTCAACA TGGTGGCCCC CGGCCGCGTG CTGATCTGCA CGGTGAAGGA TGAGGGTTCC 
TTCCACCTCA AGGACACAGC AAAGGCTCTG CTGAGGAGCC TGGGAAGTCA GGCAGGCCCT 
GCCCTTGGCT GGAGGGACAC CTGGGCCTTC GTGGGACGAA AAGGAGGTCC TGTACTTGGC 
GAGAAACATT CTAAATCTCC TGCCCTCTCC TCCTGGGGTG ACCCAGTCCT GCTGAAGACA 
GATGTGCCAC TGAGCTCAGC TGAAGAGGCT GAGTGTCACT GGGCAGATAC GGAGTTGAAC 
CGCCGCCGCA GGCGCTTCTG CAGCAAAGTG GAGGGCTACG GGAGCGTGTG CAGCTGCAAG 
GACCCGACGC CTATTGAGTT CAGCCCTGAC CCACTTCCAG ACAATAAGGT CCTCAATGTG 
CCTGTGGCTG TCATTGCAGG GAACCGGCCC AATTACCTGT ACAGGATGCT GCGCTCTCTG 
CTCTCAGCCC AAGGGGTATC TCCACAGATG ATAACGGTCT TCATTGATGG CTACTATGAG 
GAGCCAATGG ATGTGGTGGC GCTGTTTGGT CTGAGAGGCA TCCAGCATAC TCCTATCAGC 
ATCAAGAACG CCCGTGTGTC TCAGCACTAC AAGGCCAGCC TCACTGCCAC TTTCAACCTG 
TTTCCGGAGG CCAAGTTTGC TGTGGTTCTG GAAGAGGACC TGGACATTGC CGTGGATTTT 
TTCAGTTTCC TGAGCCAGTC CATCCACCTG CTGGAGGAAG ATGACAGCCT GTACTGCATC 
TCTGCCTGGA ATGACCAGGG GTATGAACAC ACAGCTGAGG ATCCAGCACT ACTGTACCGT 
GTGGAGACCA TGCCTGGGCT AGGATGGGTG CTCAGGAAAT CTTTATACAA GGAGGAGCTT 
GAGCCCAAGT GGCCCACACC AGAAAAGCTG TGGGATTGGG ACATGTGGAT GCGGATGCCG 
GAACAGCGCC GGGGCCGAGA GTGTATCATC CCGGATGTTT CCCGATCCTA CCACTTTGGC 
ATCGTCGGCC TCAACATGAA CGGCTACTTT CATGAGGCCT ACTTCAAGAA GCACAAGTTC 
AACACAGTTC CAGGTGTCCA GCTCAGGAAT GTAGACAGTC TGAAGAAAGA AGCTTATGAA 
GTGGAAATTC ACAGACTGCT CAGTGAGGCC GAGGTTTTGG ACCACAGCAA GGACCCTTGT 
GAAGACTCCT TCCTGCCAGA CACGGAGGGC CACACCTATG TGGCGTTTAT CCGAATGGAG 
ACAGATGATG ACTTCGCCAC CTGGACTCAG CTTGCCAAGT GCCTCCATAT TTGGGACCTG 
GATGTTCGTG GCAACCACCG GGGCCTGTGG AGATTATTTC GGAAGAAGAA CCACTTTTTG 
GTGGTGGGGG TCCCAGCCTC CCCTTACTCA GTGAAGAAGC CACCATCAGT CACCCCTATT 
TTCCTGGAGC CACCCCCAAA GGAAGAAGGA GCCCCAGGGG CTGCTGAACA AACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pomgnt1 ( XM_006503352.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503415.1 Mus musculus protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu33778
Clone ID Related Accession (Same CDS sequence)	XM_006503352.1
Accession Version	XM_006503352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACT GGAAGCCCAG TCCCCTCATC AAGCCCTTTG GGGCTAGGAA GAAACGGAGC 
TGGTACCTTA CCTGGAAGTA TAAACTGACA AACCAGCGGG CCCTGAGGAG ATTCTGTCAG 
ACAGGGGCTG TGCTTTTCCT GCTGGTGACC GTCATTGTCA ACATCAAGTT GATCCTGGAC 
ACTCGGAGAG CAATCAGCGA GGCCAATGAA GACCCAGAAC CAGAGCAAGA CTATGATGAG 
GCCCTAGGAC GTCTGGAGTC CCCTCGGCGC AGAGGCAGCA GTCCCCGACG GGTGCTGGAT 
GTGGAAGTAT ACTCCAGTAG AAGCAAAGTG TATGTGGCTG TGGATGGCAC CACGGTGCTC 
GAGGATGAAG CCCGGGAGCA GGGCCGAGGC ATCCATGTCA TAGTCCTCAA CCAGGCCACG 
GGCCACGTGA TGGCAAAGCG CGTGTTTGAC ACATACTCTC CTCATGAGGA TGAGGCCATG 
GTGCTGTTCC TCAACATGGT GGCCCCCGGC CGCGTGCTGA TCTGCACGGT GAAGGATGAG 
GGTTCCTTCC ACCTCAAGGA CACAGCAAAG GCTCTGCTGA GGAGCCTGGG AAGTCAGGCA 
GGCCCTGCCC TTGGCTGGAG GGACACCTGG GCCTTCGTGG GACGAAAAGG AGGTCCTGTA 
CTTGGCGAGA AACATTCTAA ATCTCCTGCC CTCTCCTCCT GGGGTGACCC AGTCCTGCTG 
AAGACAGATG TGCCACTGAG CTCAGCTGAA GAGGCTGAGT GTCACTGGGC AGATACGGAG 
TTGAACCGCC GCCGCAGGCG CTTCTGCAGC AAAGTGGAGG GCTACGGGAG CGTGTGCAGC 
TGCAAGGACC CGACGCCTAT TGAGTTCAGC CCTGACCCAC TTCCAGACAA TAAGGTCCTC 
AATGTGCCTG TGGCTGTCAT TGCAGGGAAC CGGCCCAATT ACCTGTACAG GATGCTGCGC 
TCTCTGCTCT CAGCCCAAGG GGTATCTCCA CAGATGATAA CGGTCTTCAT TGATGGCTAC 
TATGAGGAGC CAATGGATGT GGTGGCGCTG TTTGGTCTGA GAGGCATCCA GCATACTCCT 
ATCAGCATCA AGAACGCCCG TGTGTCTCAG CACTACAAGG CCAGCCTCAC TGCCACTTTC 
AACCTGTTTC CGGCTAGGGT GGGTAGTGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503415.1
CDS	180..1352
Translation	MDDWKPSPLIKPFGARKKRSWYLTWKYKLTNQRALRRFCQTGAVLFLLVTVIVNIKLILDTRRAISEANEDPEPEQDYDEALGRLESPRRRGSSPRRVLDVEVYSSRSKVYVAVDGTTVLEDEAREQGRGIHVIVLNQATGHVMAKRVFDTYSPHEDEAMVLFLNMVAPGRVLICTVKDEGSFHLKDTAKALLRSLGSQAGPALGWRDTWAFVGRKGGPVLGEKHSKSPALSSWGDPVLLKTDVPLSSAEEAECHWADTELNRRRRRFCSKVEGYGSVCSCKDPTPIEFSPDPLPDNKVLNVPVAVIAGNRPNYLYRMLRSLLSAQGVSPQMITVFIDGYYEEPMDVVALFGLRGIQHTPISIKNARVSQHYKASLTATFNLFPARVGSA

Target ORF information:

RefSeq Version	XM_006503352.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503352.1

ORF Insert Sequence:

ATGGATGACT GGAAGCCCAG TCCCCTCATC AAGCCCTTTG GGGCTAGGAA GAAACGGAGC 
TGGTACCTTA CCTGGAAGTA TAAACTGACA AACCAGCGGG CCCTGAGGAG ATTCTGTCAG 
ACAGGGGCTG TGCTTTTCCT GCTGGTGACC GTCATTGTCA ACATCAAGTT GATCCTGGAC 
ACTCGGAGAG CAATCAGCGA GGCCAATGAA GACCCAGAAC CAGAGCAAGA CTATGATGAG 
GCCCTAGGAC GTCTGGAGTC CCCTCGGCGC AGAGGCAGCA GTCCCCGACG GGTGCTGGAT 
GTGGAAGTAT ACTCCAGTAG AAGCAAAGTG TATGTGGCTG TGGATGGCAC CACGGTGCTC 
GAGGATGAAG CCCGGGAGCA GGGCCGAGGC ATCCATGTCA TAGTCCTCAA CCAGGCCACG 
GGCCACGTGA TGGCAAAGCG CGTGTTTGAC ACATACTCTC CTCATGAGGA TGAGGCCATG 
GTGCTGTTCC TCAACATGGT GGCCCCCGGC CGCGTGCTGA TCTGCACGGT GAAGGATGAG 
GGTTCCTTCC ACCTCAAGGA CACAGCAAAG GCTCTGCTGA GGAGCCTGGG AAGTCAGGCA 
GGCCCTGCCC TTGGCTGGAG GGACACCTGG GCCTTCGTGG GACGAAAAGG AGGTCCTGTA 
CTTGGCGAGA AACATTCTAA ATCTCCTGCC CTCTCCTCCT GGGGTGACCC AGTCCTGCTG 
AAGACAGATG TGCCACTGAG CTCAGCTGAA GAGGCTGAGT GTCACTGGGC AGATACGGAG 
TTGAACCGCC GCCGCAGGCG CTTCTGCAGC AAAGTGGAGG GCTACGGGAG CGTGTGCAGC 
TGCAAGGACC CGACGCCTAT TGAGTTCAGC CCTGACCCAC TTCCAGACAA TAAGGTCCTC 
AATGTGCCTG TGGCTGTCAT TGCAGGGAAC CGGCCCAATT ACCTGTACAG GATGCTGCGC 
TCTCTGCTCT CAGCCCAAGG GGTATCTCCA CAGATGATAA CGGTCTTCAT TGATGGCTAC 
TATGAGGAGC CAATGGATGT GGTGGCGCTG TTTGGTCTGA GAGGCATCCA GCATACTCCT 
ATCAGCATCA AGAACGCCCG TGTGTCTCAG CACTACAAGG CCAGCCTCAC TGCCACTTTC 
AACCTGTTTC CGGCTAGGGT GGGTAGTGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Degree of Cajal-Retzius Cell Mislocalization Correlates with the Severity of Structural Brain Defects in Mouse Models of Dystroglycanopathy.
Brain pathology (Zurich, Switzerland)26(4)465-78(2016 Jul)
Booler HS,Williams JL,Hopkinson M,Brown SC

Neurons and glia modify receptor protein-tyrosine phosphatase ? (RPTP?)/phosphacan with cell-specific O-mannosyl glycans in the developing brain.
The Journal of biological chemistry290(16)10256-73(2015 Apr)
Dwyer CA,Katoh T,Tiemeyer M,Matthews RT

Role of glycosyltransferase PomGnT1 in glioblastoma progression.
Neuro-oncology17(2)211-22(2015 Feb)
Lan J,Guo P,Lin Y,Mao Q,Guo L,Ge J,Li X,Jiang J,Lin X,Qiu Y

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

AGO61-dependent GlcNAc modification primes the formation of functional glycans on ?-dystroglycan.
Scientific reports33288(2013 Nov)
Yagi H,Nakagawa N,Saito T,Kiyonari H,Abe T,Toda T,Wu SW,Khoo KH,Oka S,Kato K

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Pomgnt1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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FAX

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